Bioremediation potential of Brevibacillus parabrevis AMB ‐CD‐2 in spinach under lead toxicity
AbstractThe present study was conducted to evaluate the impact of lead toxicity on the growth parameters of spinach and the performance of lead-resistant bacterial isolates under lead stress conditions. Out of four bacterial isolates selected for this study, only two isolates AMB-CD-2 and AMB-CD-4 were selected based on their lead tolerance ability. A polybag experiment was conducted with six treatments and four replications in spinach. The treatments included T1 (RDF + control), T2 (lead acetate), T3 (AMB-CD-2 + lead), T4 (AMB-CD-4 + lead), T5 (AMB-CD-2), and T6 (AMB-CD-4). Results showed that lead contamin...
Source: Remediation Journal - July 16, 2023 Category: Environmental Health Authors: Safina Ismail,
Ajit Kumar Mannade,
Koshlendra Tedia,
Anup Kumar Singh,
Tapas Chowdhury,
Ravindra Soni Tags: RESEARCH ARTICLE Source Type: research
Rolling circle transcription amplification-directed construction of tandem spinach-based fluorescent light-up biosensor for label-free sensing of β-glucosyltransferase activity
Biosens Bioelectron. 2023 Jul 4;237:115513. doi: 10.1016/j.bios.2023.115513. Online ahead of print.ABSTRACTβ-glucosyltransferase (β-GT) can specifically catalyze the conversion of 5-hydroxymethylcytosine (5-hmC) to 5-glucosylhydroxy methylcytosine (5-ghmC), and it is associated with the control of phage-specific gene expression by affecting transcription process in vivo and in vitro. The current strategies for β-GT assay usually involve expensive equipment, laborious treatment, radioactive hazard, and poor sensitivity. Here, we report a Spinach-based fluorescent light-up biosensor for label-free measurement of β-GT act...
Source: Biosensors and Bioelectronics - July 7, 2023 Category: Biotechnology Authors: Su Jiang Huanhuan Shi Qian Zhang Zi-Yue Wang Yan Zhang Chun-Yang Zhang Source Type: research
Rolling circle transcription amplification-directed construction of tandem spinach-based fluorescent light-up biosensor for label-free sensing of β-glucosyltransferase activity
Biosens Bioelectron. 2023 Jul 4;237:115513. doi: 10.1016/j.bios.2023.115513. Online ahead of print.ABSTRACTβ-glucosyltransferase (β-GT) can specifically catalyze the conversion of 5-hydroxymethylcytosine (5-hmC) to 5-glucosylhydroxy methylcytosine (5-ghmC), and it is associated with the control of phage-specific gene expression by affecting transcription process in vivo and in vitro. The current strategies for β-GT assay usually involve expensive equipment, laborious treatment, radioactive hazard, and poor sensitivity. Here, we report a Spinach-based fluorescent light-up biosensor for label-free measurement of β-GT act...
Source: Biosensors and Bioelectronics - July 7, 2023 Category: Biotechnology Authors: Su Jiang Huanhuan Shi Qian Zhang Zi-Yue Wang Yan Zhang Chun-Yang Zhang Source Type: research
Increased health risk assessment in different vegetables grown under untreated sewerage irrigation regime due to higher heavy metals accumulation
Environ Sci Pollut Res Int. 2023 Jul 4. doi: 10.1007/s11356-023-28413-z. Online ahead of print.ABSTRACTHeavy metals are environmental pollutants and carcinogenic for human health if ingested. In developing countries, including Pakistan, untreated sewerage water is one of the major sources of irrigation for vegetable production in the vicinities of urban areas which might be toxic to human health due to heavy metals contamination. The present study was conducted to investigate the uptake of heavy metals by sewage water application and its impact on human health. The experiment consisted of five vegetable crops (Raphanus sat...
Source: Environmental Science and Pollution Research International - July 4, 2023 Category: Environmental Health Authors: Zeshan Hassan Ammara Hassan Muhaiman Riaz Azhar Abbas Khan Sami Ul-Allah Umbreen Shehzad Muhammad Khurshid Ali Bakhsh Jawad Munawar Shah Zahid Manzoor Source Type: research
Increased health risk assessment in different vegetables grown under untreated sewerage irrigation regime due to higher heavy metals accumulation
Environ Sci Pollut Res Int. 2023 Jul 4. doi: 10.1007/s11356-023-28413-z. Online ahead of print.ABSTRACTHeavy metals are environmental pollutants and carcinogenic for human health if ingested. In developing countries, including Pakistan, untreated sewerage water is one of the major sources of irrigation for vegetable production in the vicinities of urban areas which might be toxic to human health due to heavy metals contamination. The present study was conducted to investigate the uptake of heavy metals by sewage water application and its impact on human health. The experiment consisted of five vegetable crops (Raphanus sat...
Source: Environmental Science and Pollution Research International - July 4, 2023 Category: Environmental Health Authors: Zeshan Hassan Ammara Hassan Muhaiman Riaz Azhar Abbas Khan Sami Ul-Allah Umbreen Shehzad Muhammad Khurshid Ali Bakhsh Jawad Munawar Shah Zahid Manzoor Source Type: research
Use of a taxon-specific reference database for accurate metagenomics-based pathogen detection of Listeria monocytogenes in turkey deli meat and spinach
The reliability of culture-independent pathogen detection in foods using metagenomics is contingent on the quality and composition of the reference database. The inclusion of microbial sequences from a diverse... (Source: BMC Genomics)
Source: BMC Genomics - June 27, 2023 Category: Genetics & Stem Cells Authors: Jillian Rumore, Matthew Walker, Franco Pagotto, Jessica D. Forbes, Christy-Lynn Peterson, Andrea D. Tyler, Morag Graham, Gary Van Domselaar, Celine Nadon, Aleisha Reimer and Natalie Knox Tags: Research Source Type: research
Disinfection of Clostridioides difficile on spinach with epigallocatechin-based antimicrobial solutions and sodium hypochlorite
Int J Food Microbiol. 2023 Jun 22;402:110301. doi: 10.1016/j.ijfoodmicro.2023.110301. Online ahead of print.ABSTRACTThe removal of C. difficile inoculated on fresh spinach leaves washed with antimicrobial solutions was investigated. In addition, the effect of washing solutions on the total aerobic mesophilic bacteria (TAMB) and Enterobacteriaceae in the fresh spinach was examined. The fresh spinach was washed through immersion in different concentrations (MIC, 2xMIC, and 4xMIC) of the natural disinfectant solution (NDS) consisting of EDTA, borax, and epigallocatechin gallate (EGCG) content developed in our laboratory and g...
Source: International Journal of Food Microbiology - June 26, 2023 Category: Food Science Authors: Melike Nur Tosun Gizem Taylan Yalc ın Gizem Korkmazer Murat Zorba Cengiz Caner N ükhet Nilüfer Demirel Zorba Source Type: research
Disinfection of Clostridioides difficile on spinach with epigallocatechin-based antimicrobial solutions and sodium hypochlorite
Int J Food Microbiol. 2023 Jun 22;402:110301. doi: 10.1016/j.ijfoodmicro.2023.110301. Online ahead of print.ABSTRACTThe removal of C. difficile inoculated on fresh spinach leaves washed with antimicrobial solutions was investigated. In addition, the effect of washing solutions on the total aerobic mesophilic bacteria (TAMB) and Enterobacteriaceae in the fresh spinach was examined. The fresh spinach was washed through immersion in different concentrations (MIC, 2xMIC, and 4xMIC) of the natural disinfectant solution (NDS) consisting of EDTA, borax, and epigallocatechin gallate (EGCG) content developed in our laboratory and g...
Source: International Journal of Food Microbiology - June 26, 2023 Category: Food Science Authors: Melike Nur Tosun Gizem Taylan Yalc ın Gizem Korkmazer Murat Zorba Cengiz Caner N ükhet Nilüfer Demirel Zorba Source Type: research
Far-red photosynthesis: Two charge separation pathways exist in plant Photosystem II reaction center
Biochim Biophys Acta Bioenerg. 2023 Jun 22:148994. doi: 10.1016/j.bbabio.2023.148994. Online ahead of print.ABSTRACTExistence of the alternative charge separation pathway in Photosystem II under the far-red light was induced photochemistry in Photosystem II was proposed by us on the basis of induced electron transfer reactions at cryogenic temperature 5 K. Here we extend these studies to the higher temperature range of 77-295 K with help of electron paramagnetic resonance spectroscopy. Induction of the S2 state multiline signal, oxidation of cytochrome b559 and chlorophyllZ was studied in Photosystem II membrane preparatio...
Source: Biochimica et Biophysica Acta - June 24, 2023 Category: Biochemistry Authors: Andrea Pavlou Fredrik Mokvist Stenbj örn Styring Fikret Mamedov Source Type: research
Far-red photosynthesis: Two charge separation pathways exist in plant Photosystem II reaction center
Biochim Biophys Acta Bioenerg. 2023 Jun 22:148994. doi: 10.1016/j.bbabio.2023.148994. Online ahead of print.ABSTRACTExistence of the alternative charge separation pathway in Photosystem II under the far-red light was induced photochemistry in Photosystem II was proposed by us on the basis of induced electron transfer reactions at cryogenic temperature 5 K. Here we extend these studies to the higher temperature range of 77-295 K with help of electron paramagnetic resonance spectroscopy. Induction of the S2 state multiline signal, oxidation of cytochrome b559 and chlorophyllZ was studied in Photosystem II membrane preparatio...
Source: Biochimica et Biophysica Acta - June 24, 2023 Category: Biochemistry Authors: Andrea Pavlou Fredrik Mokvist Stenbj örn Styring Fikret Mamedov Source Type: research
Heavy metals contamination in vegetables irrigated with wastewater: a case study of underdeveloping regions of Pakistan
This study contributes to the evidence-based conclusion that cultivation of vegetable with untreated wastewater should be prohi bited and required treatment of wastewater is needed. (Source: Environmental Geochemistry and Health)
Source: Environmental Geochemistry and Health - June 24, 2023 Category: Environmental Health Source Type: research
Effective nutrient removal using nutrient film technique hydroponics unit under varying nitrate nitrogen concentrations
Fabrication of hydroponic test bed AbstractSurface and groundwater are both susceptible to nitrate nitrogen contamination that has emerged as an issue around the globe in past few years. The main goal of this research was to treat nitrate-contaminated water through phytoremediation in a hydroponic system with reduced cost and also assess the root and shoot growth of plants cultivated in NFT hydroponic systems. Four different nitrate-nitrogen concentrations (30, 60, 90, and 120 mg/L) were given to hydroponic treatment unit. The results revealed that shoot growth, root growth, leaf number, and shoot numbers were increased ...
Source: Environmental Quality Management - June 20, 2023 Category: Environmental Health Authors: Manimozhi Rajalakshmi,
Valsa Remony Manoj Tags: RESEARCH ARTICLE Source Type: research
Molecules, Vol. 28, Pages 4824: Betaine as a Functional Ingredient: Metabolism, Health-Promoting Attributes, Food Sources, Applications and Analysis Methods
ovana Kojić
Betaine is a non-essential amino acid with proven functional properties and underutilized potential. The most common dietary sources of betaine are beets, spinach, and whole grains. Whole grains—such as quinoa, wheat and oat brans, brown rice, barley, etc.—are generally considered rich sources of betaine. This valuable compound has gained popularity as an ingredient in novel and functional foods due to the demonstrated health benefits that it may provide. This review study will provide an overview of the various natural sources of betaine, including different types of food produ...
Source: Molecules - June 17, 2023 Category: Chemistry Authors: Dejan Dobrijevi ć Kristian Pastor Nata ša Nastić Fatih Özogul Jelena Krulj Bojana Koki ć Elena Bartkiene Jo ão Miguel Rocha Jovana Koji ć Tags: Review Source Type: research
Molecules, Vol. 28, Pages 4780: Variation in the Main Health-Promoting Compounds and Antioxidant Capacity of Three Leafy Vegetables in Southwest China
u Li
Fen Zhang
Bo Sun
Malabar spinach (Basella alba), amaranth (Amaranthus tricolor), and sweet potato (Ipomoea batatas) are leafy vegetables found in Southwest China. The variation of chlorophyll, carotenoids, ascorbic acid, total flavonoids, phenolic compounds, and antioxidant capacity was studied in the leaves and stems of the three vegetables. The content of main health-promoting compounds and the antioxidant capacity in the leaves were higher than that in the stems, indicating that the leaves of the three vegetables possess greater nutritional value. The trend of total flavonoids in all three vegetables was si...
Source: Molecules - June 15, 2023 Category: Chemistry Authors: Yi Zhang Wenli Huang Chenlu Zhang Huanhuan Huang Shihan Yang Yiqing Wang Zhi Huang Yi Tang Xiaomei Li Huashan Lian Huanxiu Li Fen Zhang Bo Sun Tags: Article Source Type: research
Effect of organic photovoltaic and red-foil transmittance on yield, growth and photosynthesis of two spinach genotypes under field and greenhouse conditions
Photosynth Res. 2023 Jun 14. doi: 10.1007/s11120-023-01028-8. Online ahead of print.ABSTRACTThe galloping rise in global population in recent years and the accompanying increase in food and energy demands has created land use crisis between food and energy production, and eventual loss of agricultural lands to the more lucrative photovoltaics (PV) energy production. This experiment was carried out to investigate the effect of organic photovoltaics (OPV) and red-foil (RF) transmittance on growth, yield, photosynthesis and SPAD value of spinach under greenhouse and field conditions. Three OPV levels (P0: control; P1: transmi...
Source: Cell Research - June 14, 2023 Category: Cytology Authors: Uchenna Noble Ukwu Joy Udoka Agbo Onno Muller Silvia Schrey Ladislav Nedbal Yuxi Niu Matthias Meier-Gr üll Michael Uguru Source Type: research
