Multi-laboratory validation study of a real-time PCR method for detection of Salmonella in baby spinach
Food Microbiol. 2023 Sep;114:104299. doi: 10.1016/j.fm.2023.104299. Epub 2023 May 5.ABSTRACTThe FDA Bacteriological Analytical Manual (BAM) Salmonella culture method takes at least 3 days for a presumptive positive result. The FDA developed a quantitative PCR (qPCR) method to detect Salmonella from 24-h preenriched cultures, using ABI 7500 PCR system. The qPCR method has been evaluated as a rapid screening method for a broad range of foods by single laboratory validation (SLV) studies. The present multi-laboratory validation (MLV) study was aimed to measure the reproducibility of this qPCR method and compare its performanc...
Source: Food Microbiology - June 8, 2023 Category: Food Science Authors: Kaiping Deng Shizhen Steven Wang Shannon Kiener Emily Smith Kai-Shun Chen Ruiqing Pamboukian Anna Laasri Catalina Pelaez Jodie Ulaszek Matthew Kmet Antonio De Jesus Thomas Hammack Ravinder Reddy Hua Wang Source Type: research
Multi-laboratory validation study of a real-time PCR method for detection of Salmonella in baby spinach
Food Microbiol. 2023 Sep;114:104299. doi: 10.1016/j.fm.2023.104299. Epub 2023 May 5.ABSTRACTThe FDA Bacteriological Analytical Manual (BAM) Salmonella culture method takes at least 3 days for a presumptive positive result. The FDA developed a quantitative PCR (qPCR) method to detect Salmonella from 24-h preenriched cultures, using ABI 7500 PCR system. The qPCR method has been evaluated as a rapid screening method for a broad range of foods by single laboratory validation (SLV) studies. The present multi-laboratory validation (MLV) study was aimed to measure the reproducibility of this qPCR method and compare its performanc...
Source: Food Microbiology - June 8, 2023 Category: Food Science Authors: Kaiping Deng Shizhen Steven Wang Shannon Kiener Emily Smith Kai-Shun Chen Ruiqing Pamboukian Anna Laasri Catalina Pelaez Jodie Ulaszek Matthew Kmet Antonio De Jesus Thomas Hammack Ravinder Reddy Hua Wang Source Type: research
Multi-laboratory validation study of a real-time PCR method for detection of Salmonella in baby spinach
Food Microbiol. 2023 Sep;114:104299. doi: 10.1016/j.fm.2023.104299. Epub 2023 May 5.ABSTRACTThe FDA Bacteriological Analytical Manual (BAM) Salmonella culture method takes at least 3 days for a presumptive positive result. The FDA developed a quantitative PCR (qPCR) method to detect Salmonella from 24-h preenriched cultures, using ABI 7500 PCR system. The qPCR method has been evaluated as a rapid screening method for a broad range of foods by single laboratory validation (SLV) studies. The present multi-laboratory validation (MLV) study was aimed to measure the reproducibility of this qPCR method and compare its performanc...
Source: Food Microbiology - June 8, 2023 Category: Food Science Authors: Kaiping Deng Shizhen Steven Wang Shannon Kiener Emily Smith Kai-Shun Chen Ruiqing Pamboukian Anna Laasri Catalina Pelaez Jodie Ulaszek Matthew Kmet Antonio De Jesus Thomas Hammack Ravinder Reddy Hua Wang Source Type: research
Multi-laboratory validation study of a real-time PCR method for detection of Salmonella in baby spinach
Food Microbiol. 2023 Sep;114:104299. doi: 10.1016/j.fm.2023.104299. Epub 2023 May 5.ABSTRACTThe FDA Bacteriological Analytical Manual (BAM) Salmonella culture method takes at least 3 days for a presumptive positive result. The FDA developed a quantitative PCR (qPCR) method to detect Salmonella from 24-h preenriched cultures, using ABI 7500 PCR system. The qPCR method has been evaluated as a rapid screening method for a broad range of foods by single laboratory validation (SLV) studies. The present multi-laboratory validation (MLV) study was aimed to measure the reproducibility of this qPCR method and compare its performanc...
Source: Food Microbiology - June 8, 2023 Category: Food Science Authors: Kaiping Deng Shizhen Steven Wang Shannon Kiener Emily Smith Kai-Shun Chen Ruiqing Pamboukian Anna Laasri Catalina Pelaez Jodie Ulaszek Matthew Kmet Antonio De Jesus Thomas Hammack Ravinder Reddy Hua Wang Source Type: research
Multi-laboratory validation study of a real-time PCR method for detection of Salmonella in baby spinach
Food Microbiol. 2023 Sep;114:104299. doi: 10.1016/j.fm.2023.104299. Epub 2023 May 5.ABSTRACTThe FDA Bacteriological Analytical Manual (BAM) Salmonella culture method takes at least 3 days for a presumptive positive result. The FDA developed a quantitative PCR (qPCR) method to detect Salmonella from 24-h preenriched cultures, using ABI 7500 PCR system. The qPCR method has been evaluated as a rapid screening method for a broad range of foods by single laboratory validation (SLV) studies. The present multi-laboratory validation (MLV) study was aimed to measure the reproducibility of this qPCR method and compare its performanc...
Source: Food Microbiology - June 8, 2023 Category: Food Science Authors: Kaiping Deng Shizhen Steven Wang Shannon Kiener Emily Smith Kai-Shun Chen Ruiqing Pamboukian Anna Laasri Catalina Pelaez Jodie Ulaszek Matthew Kmet Antonio De Jesus Thomas Hammack Ravinder Reddy Hua Wang Source Type: research
