DNA Methylation is Involved in Sex Determination in Spinach
Biochem Genet. 2023 Nov 11. doi: 10.1007/s10528-023-10524-4. Online ahead of print.ABSTRACTDNA methylation plays a critical role in the modulation of gene expression. The role of DNA methylation in sex determination was investigated in spinach. The differentiated cytosine CpG methylation profiles of CCGG motifs were assessed with methylation sensitivity amplification polymorphism (MSAP) in spinach. Among 442 DNA fragments from four plants, 134 methylated fragments were found. Relative proportions of methylation sites were 28.8% in male plants and 31.8% in female plants. At the same time, cytosine methylation levels were hi...
Source: Biochemical Genetics - November 11, 2023 Category: Genetics & Stem Cells Authors: Keli Jia Jiaming Duan Guangqian Cheng Heng Li Shufen Li Miao Hu Source Type: research

DNA Methylation is Involved in Sex Determination in Spinach
Biochem Genet. 2023 Nov 11. doi: 10.1007/s10528-023-10524-4. Online ahead of print.ABSTRACTDNA methylation plays a critical role in the modulation of gene expression. The role of DNA methylation in sex determination was investigated in spinach. The differentiated cytosine CpG methylation profiles of CCGG motifs were assessed with methylation sensitivity amplification polymorphism (MSAP) in spinach. Among 442 DNA fragments from four plants, 134 methylated fragments were found. Relative proportions of methylation sites were 28.8% in male plants and 31.8% in female plants. At the same time, cytosine methylation levels were hi...
Source: Biochemical Genetics - November 11, 2023 Category: Genetics & Stem Cells Authors: Keli Jia Jiaming Duan Guangqian Cheng Heng Li Shufen Li Miao Hu Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research

Flagella-mediated adhesion of Escherichia coli O157:H7 to surface of stainless steel, glass and fresh produces during sublethal injury and recovery
Food Microbiol. 2024 Feb;117:104383. doi: 10.1016/j.fm.2023.104383. Epub 2023 Sep 12.ABSTRACTE. coli O157:H7 can be induced into sublethally injured (SI) state by lactic acid (LA) and regain activity in nutrient environments. This research clarified the role of flagella-related genes (fliD, fliS, cheA and motA) in adhesion of E. coli O157:H7 onto stainless steel, glass, lettuce, spinach, red cabbage and cucumber during LA-induced SI and recovery by plate counting. Results of adhesion showed improper flagellar rotation caused by the deletion of motA resulting in the decreased adhesion. Motility of wildtype determined by dia...
Source: Food Microbiology - November 2, 2023 Category: Food Science Authors: Yujun Zhai Weina Tian Kewei Chen Linshu Lan Jianquan Kan Hui Shi Source Type: research