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Therapy: Gene Therapy
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Total 490 results found since Jan 2013.
Silencing of Glucose Transporter Protein-1 by RNA Interference Inhibits Human Osteosarcoma Mg63 Cells Growth in vivo.
Abstract
While knock-down of glucose transporter protein 1 (GLUT-1) inhibited various human cancer cell growth in vitro and in vivo, including osteosarcoma cell growth in vitro, there has been no report on whether knock-down of GLUT-1 by siRNA may inhibit osteosarcoma cell growth in vivo. We hypothesized that siRNA may inhibit osteosarcoma cell growth in vivo. We introduced siRNA-GLUT-1 by lentivirus into MG63 osteosarcoma cells which were xenograted into nude mice. Immunohistochemical staining, Western blot and reverse transcriptase quantitative (RT-qPCR) were used to determine GLUT-1 protein and mRNA expression ...
Source: Technology in Cancer Research and Treatment - February 3, 2014 Category: Cancer & Oncology Authors: Jian F, Yuan F, Jiong M, Zhu XZ, Yu GR, Lu DD Tags: Technol Cancer Res Treat Source Type: research
Knockdown of ribosomal protein L39 by RNA interference inhibits the growth of human pancreatic cancer cells in vitro and in vivo.
Abstract
Pancreatic cancer remains a major unsolved health problem lacking a potent therapeutic option. Our previous studies showed that the ribosomal protein L39 (RPL39) gene was up-regulated after long-term silencing of oncogenic KRAS in pancreatic cancer PANC-1 cells, which indicated that RPL39 may be important for pancreatic cancer development and survival. In the current study, small interfering RNA (siRNA) targeting of the RPL39 gene was performed to determine the effects of the RPL39 gene on growth of pancreatic cancer PANC-1 and BxPC-3 cells in vitro and in vivo. Results from in vitro experiments showed th...
Source: Biotechnology Journal - May 1, 2014 Category: Biotechnology Authors: Li CO, Chen D, Luo M, Ge M, Zhu J Tags: Biotechnol J Source Type: research
Construction and identification of an RNA interference lentiviral vector targeting the mouse TNF-α gene.
In conclusion, RNAi lentiviral vector particles targeting the mouse TNF-α gene were successfully obtained in the present study. This method may be used to produce lentiviral vector for the in vivo study of RNAi gene therapy targeting TNF-α.
PMID: 26668629 [PubMed - as supplied by publisher]
Source: Experimental and Therapeutic Medicine - December 19, 2015 Category: Journals (General) Tags: Exp Ther Med Source Type: research
Slug inhibition increases radiosensitivity of oral squamous cell carcinoma cells by upregulating PUMA.
This study aims to investigate whether the modulation of Slug expression by siRNA affects oral squamous cell carcinoma sensitivity to X-ray irradiation through upregulating PUMA. Two oral squamous cell carcinoma cell lines (HSC3 and HSC6) were transfected with small interfering RNA (siRNA) targeting Slug and subjected to radiotherapy in vitro. After transfection with Slug siRNA, both HSC3 and HSC6 cells showed relatively lower expression of Slug and higher expression of PUMA. The Slug siRNA transfected cells showed decreased survival and proliferation rates, an increased apoptosis rate and enhanced radiosensitivity to X-r...
Source: International Journal of Oncology - June 7, 2016 Category: Cancer & Oncology Authors: Jiang F, Zhou L, Wei C, Zhao W, Yu D Tags: Int J Oncol Source Type: research
Amphiphilic small peptides for delivery of plasmid DNAs and siRNAs
This article is protected by copyright. All rights reserved.
As novel nucleic acid carriers, linear and branched amphiphilic peptides were prepared. Each peptide was compared in terms of SNALP size, stability, and delivery activities for plasmid DNA (pDNA) and siRNA. Among the five peptides, a linear peptide H‐RCL was an effective delivery carrier for both siRNA and pDNA. Introduction of a benzoyl group to H‐RCL significantly enhanced siRNA delivery, but greatly reduced pDNA delivery. These demonstrated that a small structural modification of the peptides can modulate their delivery properties.
Source: Chemical Biology and Drug Design - October 20, 2017 Category: Biology Authors: Eun ‐Kyoung Bang, Hanna Cho, Sean S‐H Jeon, Na Ly Tran, Dong‐Kwon Lim, Wooyoung Hur, Taebo Sim Tags: Research Article Source Type: research
Caged siRNAs with single cRGD modification for photo-regulation of exogenous and endogenous gene expression in cells and mice.
This study also suggested that photomodulation of target gene expression using single cRGD caged siRNA at 5' end of antisense strand RNA inhibited siRNA activity probably due to three factors: 1) trapping of cRGD modified siRNA in endosome and lysosome, 2) the steric hindrance of cRGD, 3) the binding of cRGD to its corresponding receptor.
PMID: 29727579 [PubMed - as supplied by publisher]
Source: Biomacromolecules - May 4, 2018 Category: Biochemistry Authors: Yu L, Liang D, Chen C, Tang X Tags: Biomacromolecules Source Type: research
Solid Phase Synthesis and Self ‐Assembly of Higher‐Order siRNAs and their Bioconjugates
This article is protected by copyright. All rights reserved.
Source: Chemical Biology and Drug Design - November 27, 2018 Category: Biology Authors: Christopher N. Cultrara,
Sunil Shah,
Stephen D. Kozuch,
Mayurbhai R. Patel,
David Sabatino Tags: Special Issue Article Source Type: research
Tumor Chemosensitization through Oncogene Knockdown Mediated by Unique α-Tocopherylated Cationic Geminis.
Abstract
Herein, siRNA transfection efficiency of a unique set of α-tocopherylated gemini lipids has been established in vitro and in vivo. High efficacy of oncogene silencing achieved using the biomacromolecular assembly, formed from siRNA complexes of co-liposomes containing an α-tocopherylated gemini lipid, has been utilized for tumor regression via chemosensitization. Delivery studies with the gemini bearing hydroxyethyl headgroup with octamethylene spacer (TH8S) pointed to a higher siRNA transfection efficacy than its analog without hydroxyethyl group (T8S). Owing to p53 upregulation, transfected cells show...
Source: Biomacromolecules - March 24, 2019 Category: Biochemistry Authors: Kamra M, Maiti B, Dixit A, Karande AA, Bhattacharya S Tags: Biomacromolecules Source Type: research
Lauroylated Histidine-Enriched S4 13 -PV Peptide as an Efficient Gene Silencing Mediator in Cancer Cells
ConclusionsThese encouraging results pave the way for a potential application of the C12-H5-S413-PV peptide as a promising tool in cancer gene therapy.
Source: Pharmaceutical Research - September 3, 2020 Category: Drugs & Pharmacology Source Type: research
Carbon Dots with Guanidinium and Amino Acid Functional Groups for Targeted Small Interfering RNA Delivery toward Tumor Gene Therapy
Large amino acid mimicking carbon dots with guanidinium functionalization (LAAM GUA-CDs) can be prepared to carry small interfering RNA by multiple hydrogen bonds and target to tumor tissues specifically through LAT1 receptor. After loading siBcl-2 as a therapeutic agent, LAAM GUA-CDs/siBcl-2 bring about superior antitumor effects without side effects during the gene therapy. AbstractSmall interfering RNA (siRNA)-based gene therapy represents a promising strategy for tumor treatment. Novel gene vectors that can achieve targeted delivery of siRNA to the tumor cells without causing any side effects are urgently needed. To th...
Source: Small - February 25, 2023 Category: Nanotechnology Authors: Huimin Xu,
Jianqiao Chang,
Hao Wu,
Haoyu Wang,
Wenjing Xie,
Yunchao Li,
Xiaohong Li,
Yang Zhang,
Louzhen Fan Tags: Research Article Source Type: research
Leukemia cell-targeted STAT3 silencing and TLR9 triggering generate systemic antitumor immunity
Signal transducer and activator of transcription 3 (STAT3) is an oncogene and immune checkpoint commonly activated in cancer cells and in tumor-associated immune cells. We previously developed an immunostimulatory strategy based on targeted Stat3 silencing in Toll-like receptor 9 (TLR9)-positive hematopoietic cells using CpG-small interfering RNA (siRNA) conjugates. Here, we assessed the therapeutic effect of systemic STAT3 blocking/TLR9 triggering in disseminated acute myeloid leukemia (AML). We used mouse Cbfb-MYH11/Mpl-induced leukemia model, which mimics human inv(16) AML. Our results demonstrate that intravenously del...
Source: Blood - January 2, 2014 Category: Hematology Authors: Hossain, D. M. S., Dos Santos, C., Zhang, Q., Kozlowska, A., Liu, H., Gao, C., Moreira, D., Swiderski, P., Jozwiak, A., Kline, J., Forman, S., Bhatia, R., Kuo, Y.-H., Kortylewski, M. Tags: Plenary Papers, Myeloid Neoplasia, Gene Therapy Source Type: research
Abstract 1453: MicroRNA replacement and RNAi-mediated silencing of ALK as combined targeted therapies for neuroblastoma
The effective treatment of advanced Neuroblastoma (NB) is still a challenge in pediatric oncology, because the clinical use of most therapeutics is limited by insufficient drug delivery to the tumor and high systemic toxicity. The discovery of the RNAi has great promise for anti-cancer therapeutics but, as in high-grade solid tumors a single ‘oncogene addiction’ is rare, multi-'gene' target combinations are required and a targeted delivery system is mandatory to successfully translate RNAi-based therapeutics into the clinics.
It is now ascertained the master role of ALK and related genes such as PHOX2B, able to promote...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Perri, P., Paolo, D. D., Priddy, L., Fiore, A. D., Brignole, C., Pastorino, F., Brown, D., Ponzoni, M. Tags: Molecular and Cellular Biology Source Type: research
Theranostical nanosystem‐mediated identification of an oncogene and highly effective therapy in hepatocellular carcinoma
In conclusion, the theranostic siRNA nanomedicine examined here possesses great theranostic potential for combined gene therapy and MRI diagnosis of HCC. This article is protected by copyright. All rights reserved.
Source: Hepatology - December 1, 2015 Category: Internal Medicine Authors: Yu Guo, Jing Wang, Lu Zhang, Shunli Shen, Ruomi Guo, Yang Yang, Wenjie Chen, Yiru Wang, Guihua Chen, Xintao Shuai Tags: Hepatobiliary Malignancies Source Type: research
Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy.
Authors: Huang W, Liu Z, Zhou G, Tian A, Sun N
Abstract
Bcl-2-associated athanogene 1 (Bag-1) is a positive regulator of Bcl-2 which is an anti-apoptotic gene. Bag-1 was very slightly expressed in normal tissues, but often highly expressed in many tumor tissues, particularly in colon cancer, which can promote metastasis, poor prognosis and anti-apoptotic function of colon cancer. We prepared and evaluated magnetic gold nanoparticle/Bag-1 siRNA recombinant plasmid complex, a gene therapy system, which can transfect cells efficiently, for both therapeutic effect and safety in vitro mainly by electrophoretic mobilit...
Source: Oncology Reports - January 16, 2016 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
Downregulation of Renal G Protein-Coupled Receptor Kinase Type 4 Expression via Ultrasound-Targeted Microbubble Destruction Lowers Blood Pressure in Spontaneously Hypertensive Rats Hypertension
ConclusionsTaken together, these study results indicate that UTMD‐targeted GRK4 siRNA delivery to the kidney effectively reduces D1R phosphorylation by inhibiting renal GRK4 expression, improving D1R‐mediated natriuresis and diuresis, and lowering BP, which may provide a promising novel strategy for gene therapy for hypertension.
Source: JAHA:Journal of the American Heart Association - October 5, 2016 Category: Cardiology Authors: Huang, H., Li, X., Zheng, S., Chen, Y., Chen, C., Wang, J., Tong, H., Zhou, L., Yang, J., Zeng, C. Tags: Nephrology and Kidney, High Blood Pressure, Hypertension Original Research Source Type: research
