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Therapy: Gene Therapy

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Total 490 results found since Jan 2013.

Co-delivery of siRNA and hypericin into cancer cells by hyaluronic acid modified PLGA-PEI nanoparticles.
CONCLUSION: Results demonstrated that Hy-HPP NPs could significantly enhance the tranfection efficiency of siRNA, suggesting Hy-encapsulated nanoparticle as an efficient gene carrier. The co-delivery of HIF-1α siRNA (siHIF-1α) and Hy could efficiently decrease the level of HIF-1α and increase the affinity toward necrotic tissues. Hence, this is a promising strategy for further application in radiotherapy. PMID: 26472259 [PubMed - as supplied by publisher]
Source: Drug Development and Industrial Pharmacy - February 16, 2016 Category: Drugs & Pharmacology Tags: Drug Dev Ind Pharm Source Type: research

CD25 siRNA induces Treg/Th1 cytokine expression in rat corneal transplantation models.
In conclusion, CD25 siRNA gene therapy played a protective role in corneal graft rejection via up-regulation of Treg cytokine expression and down-regulation of Th1 cytokine expression. PMID: 27567557 [PubMed - as supplied by publisher]
Source: Experimental Eye Research - August 23, 2016 Category: Opthalmology Authors: Qin Q, Luo D, Shi Y, Zhao Q, Chen Y, Wu J, Zhao M Tags: Exp Eye Res Source Type: research

siRNA delivery system based on magnetic nanovectors: Characterization and stability evaluation.
Abstract Gene therapy and particularly small interfering RNA (siRNA) is a promising therapeutic method for treatment of various human diseases, especially cancer. However the lack of an ideal delivery system limits its clinical applications. Effective anticancer drug development represents the key for translation of research advances into medicines. Previously we reported, the optimization of magnetic siRNA nanovectors (MSN) formulation based on superparamagnetic iron oxide nanoparticles (SPION) and chitosan for systemic administration. This work aimed at using rational design to further optimize and develop MSN. ...
Source: European Journal of Pharmaceutical Sciences - June 1, 2017 Category: Drugs & Pharmacology Authors: Abdelrahman M, Eyrolles LD, Alkarib SY, Hervé-Aubert K, Djemaa SB, Marchais H, Chourpa I, David S Tags: Eur J Pharm Sci Source Type: research

Ruthenium dendrimers as carriers for anticancer siRNA.
This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into...
Source: Journal of Inorganic Biochemistry - January 12, 2018 Category: Biochemistry Authors: Michlewska S, Ionov M, Maroto-Díaz M, Szwed A, Ihnatsyeu-Kachan A, Loznikova S, Shcharbin D, Maly M, Ramirez RG, de la Mata FJ, Bryszewska M Tags: J Inorg Biochem Source Type: research

Potential of siRNA-albumin complex against cancer
Publication date: Available online 27 April 2018Source: Chemico-Biological InteractionsAuthor(s): Na Liu, Yan-Hua Qi, Chuan-Tao Cheng, Wen Bin Yang, Anshoo Malhotra, Qi ZhouAbstractRNA interference is a highly specific as well as efficient technology for gene therapy application in molecular oncology. The present study was planned to develop an efficient and stable tumor selective delivery mechanism for siRNA gene therapy for the purpose of both diagnosis as well as therapy. We have used 20 Male wistar rats for the formation of colon cancer model and utilized albumin as carrier molecule for the delivery of siRNA against va...
Source: Chemico Biological Interactions - July 5, 2018 Category: Biochemistry Source Type: research

Methotrexate induced cell death mechanisms in MCF-7 adenocarcinoma breast cancer cells: Enhanced cytotoxicity following dff45-siRNA pre-treatment
Publication date: Available online 30 August 2018Source: SynergyAuthor(s): Fatemeh Kiani, Negin Rasouli, Tahereh Kashkoolinejad, Shahrokh Safarian, Seyed Jalal Zargar, Nader SheibaniAbstractThere are many efforts to diminish risks associated with the use of cancer chemotherapeutic agents through design and utilization of new strategies including gene therapy. We previously showed knockdown of dff45 or overexpression of dff40, the important apoptosis regulators, resulted in enhanced sensitization of cancer cells to chemotherapeutic agents. Here we show that pre-treatment with dff45-siRNA additively increased the overall cyt...
Source: Synergy - August 31, 2018 Category: Molecular Biology Source Type: research

Anti-HB-EGF Antibody-Mediated Delivery of siRNA to Atherosclerotic Lesions in Mice.
Authors: Tsuchida S, Matsuzaki T, Yamato M, Okuda K, Fu HY, Araki R, Sanada S, Asanuma H, Asano Y, Asakura M, Hao H, Takashima S, Kitakaze M, Sakata Y, Mekada E, Minamino T Abstract For atherosclerotic cardiovascular diseases (ACD), gene therapy may be a potential therapeutic strategy; however, lack of effective and safe methods for gene delivery to atherosclerotic plaques have limited its potential therapeutic applications. To overcome this limitation, we developed a novel antibody-based gene delivery system (anti-HB-EGF/NA vector) by chemically crosslinking antibodies against human heparin-binding epidermal growt...
Source: International Heart Journal - November 7, 2018 Category: Cardiology Tags: Int Heart J Source Type: research

Optimization of chitosan nanoparticles as an anti-HIV siRNA delivery vehicle.
Abstract Chitosan has emerged as a promising polysaccharide for gene/siRNA delivery. However, additional works will be required to modify chitosan nanoparticles. In the present study, chitosan nanoparticles were well modified to introduce anti-HIV siRNA into two mammalian cell lines, macrophage RAW 264.7 and HEK293. We first generated two stable cell lines expressing HIV-1 Tat, and then designed and generated an efficient anti-tat siRNA. The nanoparticles were prepared by using different concentrations of chitosan, polyethylenimine (PEI) and carboxymethyl dextran (CMD) in various formulations and then their physic...
Source: International Journal of Biological Macromolecules - February 6, 2019 Category: Biochemistry Authors: Mobarakeh VI, Modarressi MH, Rahimi P, Bolhassani A, Arefian E, Atyabi F, Vahabpour R Tags: Int J Biol Macromol Source Type: research

Engineered targeting tLyp-1 exosomes as gene therapy vectors for efficient delivery of siRNA into lung cancer cells
In conclusion, the targeting tLyp-1 exosomes are successfully engineered, and can be used for gene therapy with a high transfection efficiency. Therefore, the engineered targeting tLyp-1 exosomes offer a promising gene delivery platform for future cancer therapy.Graphical AbstractThe tLyp-1-lamp2b plasmid transfected HEK293T cells can secreted tumor targeting tLyp-1 exosomes. By electroporation technology, targeting tLyp-1 exosomes were loaded with siRNA. When targeting tLyp-1 exosome ruptured in cytoplasm, siRNA was loaded into the RNA-induced silencing complex (RISC). The sense (passenger) strand was degraded while the a...
Source: Asian Journal of Pharmaceutical Sciences - May 8, 2019 Category: Drugs & Pharmacology Source Type: research

Treatment of glioblastoma multiforme using combination of siRNA targeting EGFR and β‐catenin
ConclusionsSimultaneous inhibition of EGFR and β‐catenin expression could represent an effective therapy for human GBM, and warrants further study in vivo. Copyright © 2013 John Wiley & Sons, Ltd.
Source: The Journal of Gene Medicine - January 14, 2013 Category: Genetics & Stem Cells Authors: Kui Wang, James O. Park, Miqin Zhang Tags: Research Article Source Type: research

Combinational delivery of c-myc siRNA and nucleoside analogs in a single, synthetic nanocarrier for targeted cancer therapy.
In this study, we developed a Lipid/Calcium/Phosphate (LCP) nanoparticle that combines chemotherapy with gene therapy. By encapsulating a chemodrug, gemcitabine monophosphate (GMP), and siRNA specific to the undruggable cMyc oncogene (cMyc siRNA) into a single nano-sized vesicle and systemically administering them to nude mice, we achieved potent anti-tumor activity in both subcutaneous and orthotopic models of NSCLC. The improvements in therapeutic response over either cMyc siRNA or GMP therapy alone, were demonstrated by the ability to effectively induce the apoptosis of tumor cells and the significant reduction of proli...
Source: Biomaterials - August 8, 2013 Category: Materials Science Authors: Zhang Y, Peng L, Mumper RJ, Huang L Tags: Biomaterials Source Type: research

Self-assembling gelling formulation based on a crystalline-phase liquid as a non-viral vector for siRNA delivery.
Abstract Liquid crystalline systems (LCSs) form interesting drug delivery systems. These include in situ gelling delivery systems, which present several advantages for use as self-assembling systems for local drug delivery. The aim of this study was to develop and characterize in situ gelling delivery systems for local siRNA delivery. The influence of the components that form the systems was investigated, and the systems were characterized by polarized light microscopy, small angle X-ray scattering (SAXS), swelling studies, assays of their ability to form a complex with genes and of the stability of the genes in t...
Source: European Journal of Pharmaceutical Sciences - April 8, 2014 Category: Drugs & Pharmacology Authors: Borgheti-Cardoso LN, Depieri LV, Diniz H, Calzzani RA, Fantini MC, Iyomasa MM, Vicentini FT, Bentley MV Tags: Eur J Pharm Sci Source Type: research

Dual Receptor-Specific Peptides Modified Liposomes as VEGF siRNA Vector for Tumor-Targeting Therapy.
Abstract Tumor angiogenesis involves multiple signaling pathways that provide potential therapeutic targets to inhibit tumor growth and metastasis. Regarding the significant role of vascular endothelial growth factor (VEGF) in angiogenesis and tumor progression, VEGF sequence-specific small interfering RNA (siRNA) for antiangiogenic tumor therapy are under development. In the present study, a dual-modified liposomes (At-Lp) was designed by attaching two receptor-specific peptides, i.e. low-density lipoprotein receptor-related protein receptor (Angiopep) for brain tumor targeting and neuropilin-1 receptor (tLyP-1) ...
Source: Current Gene Therapy - June 12, 2014 Category: Genetics & Stem Cells Authors: Yang Z, Xiang B, Dong D, Wang Z, Jingquan L, Xianrong Q Tags: Curr Gene Ther Source Type: research

Lentivirus vectors construction of SiRNA targeting interference GPC3 gene and its biological effects on liver cancer cell lines Huh-7
Conclusions The targeted GPC3 siRNA can effectively inhibit the expression of GPC3.
Source: Asian Pacific Journal of Tropical Medicine - October 12, 2014 Category: Tropical Medicine Source Type: research