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Total 1197 results found since Jan 2013.

The NADPH oxidase NOX4 promotes the directed migration of endothelial cells by stabilizing vascular endothelial growth factor receptor 2 protein Protein Synthesis and Degradation
Directed migration of endothelial cells (ECs) is an important process during both physiological and pathological angiogenesis. The binding of vascular endothelial growth factor (VEGF) to VEGF receptor-2 (VEGFR-2) on the EC surface is necessary for directed migration of these cells. Here, we used TAXIScan, an optically accessible real-time horizontal cell dynamics assay approach, and demonstrate that reactive oxygen species (ROS)-producing NADPH oxidase 4 (NOX4), which is abundantly expressed in ECs, mediates VEGF/VEGFR-2-dependent directed migration. We noted that a continuous supply of endoplasmic reticulum (ER)-retained ...
Source: Journal of Biological Chemistry - August 13, 2020 Category: Chemistry Authors: Kei Miyano, Shuichiro Okamoto, Akira Yamauchi, Chikage Kawai, Mizuho Kajikawa, Takuya Kiyohara, Minoru Tamura, Masahiko Taura, Futoshi Kuribayashi Tags: Protein Synthesis and Degradation Source Type: research

Exosome-Mimetic Supramolecular Vesicles with Reversible and Controllable Fusion and Fission.
Abstract The fusion and fission behaviors of exosomes are essential for the cell-to-cell communication. Developing exosome-mimetic vesicles with such behaviors is of vital importance, but still remains a big challenge. Herein, we present an artificial supramolecular vesicle that exhibits redox-modulated reversible fusion-fission functions. These vesicles tend to fuse together and form large-sized vesicles upon oxidation, while undergo a fission process and return to small-sized vesicles through reduction. Noteworthy, the aggregation-induced emission (AIE) characteristics of the supramolecular building blocks enabl...
Source: Angewandte Chemie - August 9, 2020 Category: Chemistry Authors: Li J, Peng K, Li Y, Wang J, Huang J, Yan Y, Wang D, Tang BZ Tags: Angew Chem Int Ed Engl Source Type: research

Tumor Triggered Disassembly of Multiple-Agent-Therapy Probe for Efficient Cellular Internalization.
Abstract Integration of multiple agent therapy (MAT) into one probe has been considered as a promising approach to improve therapeutic efficiency for cancer treatment. However, MAT probe, if entering the cell as a whole, might not be optimal for each individual therapeutic agent (with different physicochemical properties), to achieve their best performance, which hinders the strategy optimization. Here, we report a peptide-conjugated-AIEgen (FC-PyTPA): upon loading with siRNA, it can be self-assembled into FC siRNA -PyTPA. When approaching the region near targeting tumor cells, FC siRNA -PyTPA responds to extracel...
Source: Angewandte Chemie - July 27, 2020 Category: Chemistry Authors: Yang J, Dai J, Wang Q, Cheng Y, Guo J, Zhao Z, Hong Y, Lou X, Xia F Tags: Angew Chem Int Ed Engl Source Type: research

Correction: A novel CXCR3-B chemokine receptor-induced growth-inhibitory signal in cancer cells is mediated through the regulation of Bach-1 protein and Nrf2 protein nuclear translocation. Additions and Corrections
VOLUME 289 (2014) PAGES 3126–3137There was an error in Fig 6C. The dot plot in panel 1 (Control siRNA + Vector) was mistakenly duplicated with the dot plot in panel 4 (Bach-1 siRNA + CXCR3-B). However, the numbers of the % apoptotic cells were correct. This error happened inadvertently during the preparation of the figure panels. This error has now been corrected and does not affect the results or conclusions of the work reported in this study. The authors apologize for this error.jbc;295/30/10509/F6F1F6Figure 6.
Source: Journal of Biological Chemistry - July 23, 2020 Category: Chemistry Authors: Murugabaskar Balan, Soumitro Pal Tags: Additions and Corrections Source Type: research

Molecules, Vol. 25, Pages 3344: Synthesis of Nucleobase-Modified RNA Oligonucleotides by Post-Synthetic Approach
szczynska The chemical synthesis of modified oligoribonucleotides represents a powerful approach to study the structure, stability, and biological activity of RNAs. Selected RNA modifications have been proven to enhance the drug-like properties of RNA oligomers providing the oligonucleotide-based therapeutic agents in the antisense and siRNA technologies. The important sites of RNA modification/functionalization are the nucleobase residues. Standard phosphoramidite RNA chemistry allows the site-specific incorporation of a large number of functional groups to the nucleobase structure if the building blocks are synthetic...
Source: Molecules - July 22, 2020 Category: Chemistry Authors: Karolina Bartosik Katarzyna Debiec Anna Czarnecka Elzbieta Sochacka Grazyna Leszczynska Tags: Review Source Type: research

Corrigendum: Systemic Delivery of Bc12-Targeting siRNA by DNA Nanoparticles Suppresses Cancer Cell Growth.
PMID: 32691956 [PubMed - in process]
Source: Angewandte Chemie - July 22, 2020 Category: Chemistry Authors: Rahman MA, Wang P, Zhao Z, Wang D, Nannapaneni S, Zhang C, Chen Z, Griffith CC, Hurwitz SJ, Chen ZG, Ke Y, Shin DM Tags: Angew Chem Int Ed Engl Source Type: research

Photoregulation of Gene Expression with Amantadine-modified Caged siRNAs Through Host/Guest Interaction.
Abstract RNA interference (RNAi) is an essential and powerful tool for targeting and verifying specific gene functions. Conditional control of siRNA activity, especially using light activation, is a potential method to regulate target gene expression and functions. Here, a series of photolabile siRNAs with amantadine modification were rationally designed and developed through host/guest interaction of amantadine and β-cyclodextrin derivatives to enhance the blocking effect of siRNA binding and/or processing. These caged siRNAs with amantadine modification at the 5'end of antisense-strand RNA were efficiently inac...
Source: Chemistry - July 16, 2020 Category: Chemistry Authors: Zhang J, Jing N, Fan X, Tang X Tags: Chemistry Source Type: research

Histone deacetylase 1 regulates the malignancy of oral cancer cells via miR-154-5p/PCNA axis.
Authors: Lv Y, Lu J, Liu X, Miao S, Li B, Pei R, Xiang C Abstract Histone deacetylases (HDACs) can regulate the progression of various cancers, while their roles in oral cancer cells are not well known. Our present study found that the HDAC1 was over expressed in oral squamous cell carcinoma (OSCC) cells and tissues. Targeted inhibition of HDAC1 via its specific inhibitor PCI24781 or siRNA can inhibit the proliferation of OSCC cells and increase their sensitivity to the chemo-sensitivity such as doxorubicin (Dox) treatment. HDAC1 can regulate the expression of proliferating cell nuclear antigen (PCNA) via decreasin...
Source: Biological Chemistry - June 20, 2020 Category: Chemistry Tags: Biol Chem Source Type: research

Optical Control of Gene Silencing by Azobenzene-Modified siRNA
Synfacts 2020; 16: 0852- DOI: 10.1055/s-0040-1707867 © Georg Thieme Verlag Stuttgart · New YorkArticle in Thieme eJournals: Table of contents  |  Full text
Source: Synfacts - June 16, 2020 Category: Chemistry Tags: Chemistry in Medicine and Biology Source Type: research

Molecules, Vol. 25, Pages 2714: Conjugation of Doxorubicin to siRNA Through Disulfide-based Self-immolative Linkers
Debart Co-delivery systems of siRNA and chemotherapeutic drugs have been developed as an attractive strategy to optimize the efficacy of chemotherapy towards cancer cells with multidrug resistance. In these typical systems, siRNAs are usually associated to drugs within a carrier but without covalent interactions with the risk of a premature release and degradation of the drugs inside the cells. To address this issue, we propose a covalent approach to co-deliver a siRNA-drug conjugate with a redox-responsive self-immolative linker prone to intracellular glutathione-mediated disulfide cleavage. Herein, we report the use...
Source: Molecules - June 10, 2020 Category: Chemistry Authors: Florian Gauthier Jean-R émi Bertrand Jean-Jacques Vasseur Christelle Dupouy Fran çoise Debart Tags: Article Source Type: research

Molecules, Vol. 25, Pages 2692: Lipid and Polymer-Based Nanoparticle siRNA Delivery Systems for Cancer Therapy
les RNA interference (RNAi) uses small interfering RNAs (siRNAs) to mediate gene-silencing in cells and represents an emerging strategy for cancer therapy. Successful RNAi-mediated gene silencing requires overcoming multiple physiological barriers to achieve efficient delivery of siRNAs into cells in vivo, including into tumor and/or host cells in the tumor micro-environment (TME). Consequently, lipid and polymer-based nanoparticle siRNA delivery systems have been developed to surmount these physiological barriers. In this article, we review the strategies that have been developed to facilitate siRNA survival in the ci...
Source: Molecules - June 9, 2020 Category: Chemistry Authors: Francesco Mainini Michael R. Eccles Tags: Review Source Type: research

Chronic treatment with the complex I inhibitor MPP+ depletes endogenous PTEN-induced kinase 1 (PINK1) via up-regulation of Bcl-2-associated athanogene 6 (BAG6) Neurobiology
Mitochondrial dysfunction is implicated in sporadic and familial Parkinson's disease (PD). However, the mechanisms that impair homeostatic responses to mitochondrial dysfunction remain unclear. Previously, we found that chronic, low-dose administration of the mitochondrial complex I inhibitor 1-methyl-4-phenylpyridinium (MPP+) dysregulates mitochondrial fission–fusion, mitophagy, and mitochondrial biogenesis. Given that PTEN-induced kinase 1 (PINK1) regulates mitochondrial function, dynamics, and turnover, we hypothesized that alterations in endogenous PINK1 levels contribute to depletion of mitochondria during chronic c...
Source: Journal of Biological Chemistry - June 4, 2020 Category: Chemistry Authors: Manish Verma, Jianhui Zhu, Kent Z. Q. Wang, Charleen T. Chu Tags: Molecular Bases of Disease Source Type: research

Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle Cell Biology
Chikungunya fever is a re-emerging zoonotic disease caused by chikungunya virus (CHIKV), a member of the Alphavirus genus in the Togaviridae family. Only a few studies have reported on the host factors required for intracellular CHIKV trafficking. Here, we conducted an imaging-based siRNA screen to identify human host factors for intracellular trafficking that are involved in CHIKV infection, examined their interactions with CHIKV proteins, and investigated the contributions of these proteins to CHIKV infection. The results of the siRNA screen revealed that host endosomal sorting complexes required for transport (ESCRT) pr...
Source: Journal of Biological Chemistry - June 4, 2020 Category: Chemistry Authors: Shiho Torii, Yasuko Orba, Michihito Sasaki, Koshiro Tabata, Yuji Wada, Michael Carr, Jody Hobson-Peters, Roy A. Hall, Ayato Takada, Takasuke Fukuhara, Yoshiharu Matsuura, William W. Hall, Hirofumi Sawa Tags: Microbiology Source Type: research

Dual-targeting RNA nanoparticles for efficient delivery of polymeric siRNA to cancer cells
Chem. Commun., 2020, Advance Article DOI: 10.1039/D0CC01848A, CommunicationTaehyung Kim, Ha Na Hyun, Roun Heo, Keonwook Nam, Kyungjik Yang, Young Min Kim, Yoon Suk Lee, Jae Yoon An, Jae Hyung Park, Ki Young Choi, Young Hoon Roh A new dual-targeting polymeric siRNA nanoparticle (Dual-PSNP) was developed. The Dual-PSNP showed significantly enhanced cancer-specific intracellular delivery, gene regulation efficacy, and apoptosis-mediated cytotoxicity through additive receptor-mediated interactions of the two ligands. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this ...
Source: RSC - Chem. Commun. latest articles - May 29, 2020 Category: Chemistry Authors: Taehyung Kim Source Type: research

The mRNA encoding the JUND tumor suppressor detains nuclear RNA-binding proteins to assemble polysomes that are unaffected by mTOR Protein Synthesis and Degradation
We present evidence from reciprocal immunoprecipitation experiments indicating that NCBP1 and RHA both are components of messenger ribonucleoproteins in several cell types. Moreover, tandem affinity and RT–quantitative PCR results revealed that JUND mRNA is a component of a previously unknown ribonucleoprotein complex. Results from the tandem IP indicated that another component of the JUND-containing ribonucleoprotein complex is NCBP3, a recently identified ortholog of NCBP2/CBP20. We also found that NCBP1, NCBP3, and RHA, but not NCBP2, are components of JUND-containing polysomes. Mutational analysis uncovered two dsRNA...
Source: Journal of Biological Chemistry - May 28, 2020 Category: Chemistry Authors: Gatikrushna Singh, Sarah E. Fritz, Bradley Seufzer, Kathleen Boris-Lawrie Tags: Cell Biology Source Type: research