Photoregulation of Gene Expression with Amantadine-modified Caged siRNAs Through Host/Guest Interaction.

Photoregulation of Gene Expression with Amantadine-modified Caged siRNAs Through Host/Guest Interaction. Chemistry. 2020 Jul 17;: Authors: Zhang J, Jing N, Fan X, Tang X Abstract RNA interference (RNAi) is an essential and powerful tool for targeting and verifying specific gene functions. Conditional control of siRNA activity, especially using light activation, is a potential method to regulate target gene expression and functions. Here, a series of photolabile siRNAs with amantadine modification were rationally designed and developed through host/guest interaction of amantadine and β-cyclodextrin derivatives to enhance the blocking effect of siRNA binding and/or processing. These caged siRNAs with amantadine modification at the 5'end of antisense-strand RNA were efficiently inactivated due to the host/guest interaction of amantadine and β-cyclodextrin. Photomodulation of gene silencing activity of these amantadine-modified caged siRNAs targeting both exogenous and endogenous genes was successfully achieved with low leaking activity, which indicated that host/guest interaction could be a new strategy for developing new caged siRNAs for gene photoregulation. PMID: 32678486 [PubMed - as supplied by publisher]
Source: Chemistry - Category: Chemistry Authors: Tags: Chemistry Source Type: research