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Total 1545 results found since Jan 2013.

NADPH oxidase p47phox siRNA attenuates adventitial fibroblasts proliferation and migration in apoE(-/-) mouse
Conclusion: p47phox plays a critical role in the regulation of adventitial fibroblast proliferation and migration and may be a new therapeutic target for neointimal hyperplasia.
Source: Journal of Translational Medicine - January 28, 2015 Category: Research Authors: Fang XuYing LiuLei ShiWei LiuLi ZhangHongjing CaiJie QiYong CuiWeichen WangYejia Hu Source Type: research

Attenuated Salmonella as a Delivery System for siRNA-Based Tumor Therapy
This technology comprises live, attenuated Salmonella strains as a delivery system for small interfering double-stranded RNA (siRNA)-based tumor therapy. The inventors' data provide the first convincing evidence that Salmonella can be used for delivering plasmid-based siRNAs into tumors growing in vivo. Claimed in the related patent application are methods of inhibiting the growth or reducing the volume of solid cancer tumors using the si-RNA constructs directed against genes that promote tumor survival and cancer cell growth. The Stat3-siRNAs carried by an attenuated S. typhimurium described in the application exhibit tum...
Source: NIH OTT Licensing Opportunities - January 9, 2009 Category: Research Authors: admin Source Type: research

Primary and secondary siRNA synthesis triggered by RNAs from food bacteria in the ciliate Paramecium tetraurelia
In various organisms, an efficient RNAi response can be triggered by feeding cells with bacteria producing double-stranded RNA (dsRNA) against an endogenous gene. However, the detailed mechanisms and natural functions of this pathway are not well understood in most cases. Here, we studied siRNA biogenesis from exogenous RNA and its genetic overlap with endogenous RNAi in the ciliate Paramecium tetraurelia by high-throughput sequencing. Using wild-type and mutant strains deficient for dsRNA feeding we found that high levels of primary siRNAs of both strands are processed from the ingested dsRNA trigger by the Dicer Dcr1, th...
Source: Nucleic Acids Research - February 16, 2015 Category: Research Authors: Carradec, Q., Gotz, U., Arnaiz, O., Pouch, J., Simon, M., Meyer, E., Marker, S. Tags: RNA Source Type: research

Attenuated Salmonella as a Delivery System for siRNA-Based Tumor Therapy
This technology comprises live, attenuated Salmonella strains as a delivery system for small interfering double-stranded RNA (siRNA)-based tumor therapy. The inventors' data provide the first convincing evidence that Salmonella can be used for delivering plasmid-based siRNAs into tumors growing in vivo. Claimed in the related patent application are methods of inhibiting the growth or reducing the volume of solid cancer tumors using the si-RNA constructs directed against genes that promote tumor survival and cancer cell growth. The Stat3-siRNAs carried by an attenuated S. typhimurium described in the application exhibit tum...
Source: NIH OTT Licensing Opportunities - January 9, 2009 Category: Research Authors: admin Source Type: research

Nanomaterials as Non-viral siRNA Delivery Agents for Cancer Therapy.
Authors: Singh S Abstract Gene therapy has been recently shown as a promising tool for cancer treatment as nanotechnology-based safe and effective delivery methods are developed. Generally, genes are wrapped up in extremely tiny nanoparticles which could be taken up easily by cancer cells, not to their healthy neighboring cells. Several nanoparticle systems have been investigated primarily to address the problems involved in other methods of gene delivery and observed improved anticancer efficacy suggesting that nanomedicine provides novel opportunities to safely deliver genes, thus treat cancer. In this review, va...
Source: BioImpacts - December 13, 2015 Category: Research Tags: Bioimpacts Source Type: research

SKI2 mediates degradation of RISC 5'-cleavage fragments and prevents secondary siRNA production from miRNA targets in Arabidopsis
Small regulatory RNAs are fundamental in eukaryotic and prokaryotic gene regulation. In plants, an important element of post-transcriptional control is effected by 20–24 nt microRNAs (miRNAs) and short interfering RNAs (siRNAs) bound to the ARGONAUTE1 (AGO1) protein in an RNA induced silencing complex (RISC). AGO1 may cleave target mRNAs with small RNA complementarity, but the fate of the resulting cleavage fragments remains incompletely understood. Here, we show that SKI2, SKI3 and SKI8, subunits of a cytoplasmic cofactor of the RNA exosome, are required for degradation of RISC 5', but not 3'-cleavage fragments in A...
Source: Nucleic Acids Research - December 15, 2015 Category: Research Authors: Branscheid, A., Marchais, A., Schott, G., Lange, H., Gagliardi, D., Andersen, S. U., Voinnet, O., Brodersen, P. Tags: RNA Source Type: research

Visualization of self-delivering hydrophobically modified siRNA cellular internalization
siRNAs are a new class of therapeutic modalities with promising clinical efficacy that requires modification or formulation for delivery to the tissue and cell of interest. Conjugation of siRNAs to lipophilic groups supports efficient cellular uptake by a mechanism that is not well characterized. Here we study the mechanism of internalization of asymmetric, chemically stabilized, cholesterol-modified siRNAs (sd-rxRNAs®) that efficiently enter cells and tissues without the need for formulation. We demonstrate that uptake is rapid with significant membrane association within minutes of exposure followed by the formation ...
Source: Nucleic Acids Research - January 8, 2017 Category: Research Authors: Ly, S., Navaroli, D. M., Didiot, M.-C., Cardia, J., Pandarinathan, L., Alterman, J. F., Fogarty, K., Standley, C., Lifshitz, L. M., Bellve, K. D., Prot, M., Echeverria, D., Corvera, S., Khvorova, A. Tags: Chemical Biology and Nucleic Acid Chemistry Source Type: research

Peptide carriers to the rescue: Overcoming the barriers to siRNA delivery for cancer treatment
Cancer is a significant health concern worldwide and its clinical treatment presents many challenges. Consequently, much research effort has focused on the development of new anticancer drugs to combat this disease. One area of exploration, in particular, has been in the therapeutic application of RNA interference (RNAi). Although RNAi appears to be an attractive therapeutic tool for the treatment of cancer, one of the primary obstacles towards its pervasive use in the clinic has been cell/tissue type-specific cytosolic delivery of therapeutic small interfering RNA (siRNA) molecules.
Source: Translational Research - July 28, 2019 Category: Research Authors: James C. Cummings, Haiwen Zhang, Andrew Jakymiw Tags: Review Article Source Type: research

Microencapsulated Multifunctionalized Graphene Oxide Equipped with Chloroquine for Efficient and Sustained siRNA Delivery
Biomed Res Int. 2022 Apr 6;2022:5866361. doi: 10.1155/2022/5866361. eCollection 2022.ABSTRACTA multifunctionalized graphene oxide (GO)-based carrier with conjugation of aminated-polyethylene glycol (PEG-diamine), octaarginine (R8), and folic acid (FA), which also contains chloroquine (CQ), a lysosomotropic agent, is introduced. The cellular uptake mechanisms and intracellular targeting of FA-functionalized nanocarriers are examined. The localized releases of CQ and siRNA intracellular delivery are evaluated. Microencapsulation of the nanocarrier complexed with genes in layer-by-layer coating of alginate microbeads is also ...
Source: Biomed Res - April 26, 2022 Category: Research Authors: Rana Imani Satya Prakash Hojatollah Vali John F Presley Shahab Faghihi Source Type: research

HDAC inhibition activates the apoptosome via Apaf1 upregulation in hepatocellular carcinoma
Conclusions The present data show that siRNA-knockdown of HDAC1–3 plays a major role in mediating apoptotic response to HDAC inhibitors through regulation of Apaf1.
Source: European Journal of Medical Research - June 23, 2016 Category: Research Source Type: research

Effects of RNA interference combined with ultrasonic irradiation and SonoVue microbubbles on expression of STAT3 gene in keratinocytes of psoriatic lesions
SummaryThe most effective sequence of small interfering RNA (siRNA) silencing STAT3 of psoriatic keratinocytes (KCs) was screened out, and the effects of the most effective siRNA combined with ultrasonic irradiation and SonoVue microbubbles on the expression of STAT3 of KCs and the dose- and time-response were investigated. Three chemically-synthetic siRNAs targeting STAT3 carried by Lipofectamine 3000 were transfected into KCs, and the effects on STAT3 expression were detected, then the most effective siRNA was selected for the subsequent experiments. The negative controls of siRNA (siRNA-NC) labeled with Cy3 carried by L...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - April 1, 2017 Category: Research Source Type: research

Improved asymmetry prediction for short interfering RNAs
In the development of RNA interference therapeutics, merely selecting short interfering RNA (siRNA) sequences that are complementary to the mRNA target does not guarantee target silencing. Current algorithms for selecting siRNAs rely on many parameters, one of which is asymmetry, often predicted through calculation of the relative thermodynamic stabilities of the two ends of the siRNA. However, we have previously shown that highly active siRNA sequences are likely to have particular nucleotides at each 5′‐end, independently of their thermodynamic asymmetry. Here, we describe an algorithm for predicting highly active si...
Source: FEBS Journal - January 2, 2014 Category: Research Authors: Amanda P. Malefyt, Ming Wu, Daniel B. Vocelle, Sean J. Kappes, Stephen D. Lindeman, Christina Chan, S. Patrick Walton Tags: Original Article Source Type: research

Biodistribution and Toxicity Assessment of Superparamagnetic Iron Oxide Nanoparticles In Vitro and In Vivo
This study aimed to examine the toxicity and biodistribution of SPION coated with polyethylenimine (PEI) (SPION-PEI) designed for small interfering RNA (siRNA) delivery bothin vitro andin vivo. SPION-PEI/siRNA complexes were prepared at different weight ratios. Cytotoxic effects of SPION-PEI/siRNA on HSC-T6 cell viability were determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). Rats were divided into three groups: a control group, a normal-saline group and a SPION-PEI/siRNA group. After a single intravenous injection,in vivo nanoparticle biodistribution and accumulation were evaluated ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2018 Category: Research Source Type: research

Effect of N/P ratios on physicochemical stability, cellular association, and gene silencing efficiency for trimethyl chitosan/small interfering RNA complexes
In this study, the influence of the N/P ratio on TMC/siRNA complexes was systematically investigated and TMC was found to be an effective vector for siRNA delivery using optimized formulations.
Source: Journal of Bioactive and Compatible Polymers - November 7, 2013 Category: Research Authors: Liu, X., Ma, L., Qin, W., Gao, C. Tags: Original Articles Source Type: research