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Total 1545 results found since Jan 2013.

Conjugation of Palmitic Acid Improves Potency and Longevity of siRNA Delivered via Endosomolytic Polymer Nanoparticles.
This article is protected by copyright. All rights reserved. PMID: 25641816 [PubMed - as supplied by publisher]
Source: Biomed Res - January 31, 2015 Category: Research Authors: Sarett SM, Kilchrist KV, Miteva M, Duvall CL Tags: J Biomed Mater Res A Source Type: research

Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells
Conclusions The combination of BCL11A siRNA and VCR increases apoptosis in SUDHL6 cells. Our study implies that BCL11A siRNA in combination with VCR may be a useful approach for improving effective treatment for B cell lymphoma.
Source: European Journal of Medical Research - June 24, 2014 Category: Research Source Type: research

siRNA enhances DNA-mediated interferon lambda-1 response through crosstalk between RIG-I and IFI16 signalling pathway
In addition to silencing specific genes, small interfering RNA (siRNA) transfection is also associated with the non-specific induction of inflammatory cytokines and type I interferon. Those so-called "off-target" effects have considerable implications for the interpretation of in vitro studies and clinical application of siRNA. The present study attempted to develop a better understanding of the mechanism involved in these off target effects. Synthesized siRNA significantly enhances DNA-mediated interferon lambda-1 response (IFN-1/IL-29), a newly characterized antiviral interferon in non-immune or primary immune cells. Thi...
Source: Nucleic Acids Research - December 28, 2013 Category: Research Authors: Sui, H., Zhou, M., Chen, Q., Lane, H. C., Imamichi, T. Tags: RNA Source Type: research

Role of TRIF Small Interference RNA (siRNA) in Chronic Experimental Allergic Encephalomyelitis (EAE).
CONCLUSIONS The expression of TRIF can be down-regulated by siRNA, thereby alleviating the severity of EAE via its inhibition of interleukin and cytokine release. This may provide new insights for future treatment of MS. PMID: 26324415 [PubMed - in process]
Source: Medical Science Monitor - September 4, 2015 Category: Research Tags: Med Sci Monit Source Type: research

Inhibition of HBV replication by delivering the dual-gene expression vector pHsa-miR16-siRNA in HepG2.2.15 cells
This study aimed to construct the dual-gene expression vector pHsa-miR16-siRNA which can express human miR-16 and HBV X siRNA, and examine its regulatory effect on HBV gene expression in the HepG2.2.15 cell line. The expression vectors siR-1583 and pHsa-miR16-siRNA were designed and constructed. HepG2.2.15 cells were transfected with the empty vector, siR-1583, pmiR-16 and pHsa-miR16-siRNA, respectively. ELISA was performed to measure the expression of HBsAg and HBeAg in the culture supernatant 48 and72 h post transfection. Fluorescence quantitative PCR was used to measure the HBV mRNA degradation efficiency and HBV DNA co...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2017 Category: Research Source Type: research

A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress
We reported that non-targeting siRNA (NT-siRNA) stress induces non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) expression to cooperate with exoribonuclease XRN2 for releasing the stress [Wei,P.C., Lo,W.T., Su,M.I., Shew,J.Y. and Lee,W.H. (2011) Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nucleic Acids Res., 40, 323–332]. However, how NT-siRNA stress inducing NPGPx expression remains elusive. In this communication, we showed that the proximal promoter of NPGPx contained a mixed G-quadruplex (G4) structure, and dis...
Source: Nucleic Acids Research - February 1, 2013 Category: Research Authors: Wei, P.-C., Wang, Z.-F., Lo, W.-T., Su, M.-I., Shew, J.-Y., Chang, T.-C., Lee, W.-H. Tags: Gene Regulation, Chromatin and Epigenetics Source Type: research

Recent Developments in Nanoparticle-Based siRNA Delivery for Cancer Therapy.
Abstract RNA interference (RNAi) is a gene regulation mechanism initiated by RNA molecules that enables sequence-specific gene silencing by promoting degradation of specific mRNAs. Molecular therapy using small interfering RNA (siRNA) has shown great therapeutic potential for diseases caused by abnormal gene overexpression or mutation. The major challenges to application of siRNA therapeutics include the stability and effective delivery of siRNA in vivo. Important progress in nanotechnology has led to the development of efficient siRNA delivery systems. In this review, the authors discuss recent advances in nanopa...
Source: Biomed Res - July 16, 2013 Category: Research Authors: Lee JM, Yoon TJ, Cho YS Tags: Biomed Res Int Source Type: research

Innate immunity activation involved in unprotected porcine auto-transplant kidneys preserved by naked caspase-3 siRNA
Conclusions: The activation of innate immunity with amplified inflammatory responses in the caspase-3 siRNA preserved auto-transplant kidneys are associated with increased TLR3, TLR7 and PKR, which might be due to complementary systemic feedback, although persistent actions initiated by short-acting caspase-3 siRNA cannot be completely ruled out. These results provided valuable evidence to guide future siRNA design and pre-clinic studies.
Source: Journal of Translational Medicine - September 13, 2013 Category: Research Authors: Cheng YangLong LiYinjia XueZitong ZhaoTian ZhaoYichen JiaRuiming RongMing XuMichael NicholsonTongyu ZhuBin Yang Source Type: research

Live cell imaging of duplex siRNA intracellular trafficking
Intracellular distribution of siRNA after in vitro transfection typically depends on lipopolyplexes, which must release the siRNA into the cytosol. Here, the fate of siRNAs was monitored by FRET-based live cell imaging. Subsequent to in situ observation of uptake and release processes, this approach allowed the observation of a number of hitherto uncharacterized intracellular distribution and degradation processes, commencing with a burst of endosomal releases, followed, in some cases, by fast siRNA influx into the nucleus. The continued observation of intact siRNA against a background of free fluorophores resulting from a...
Source: Nucleic Acids Research - May 19, 2015 Category: Research Authors: Hirsch, M., Helm, M. Tags: RNA Source Type: research

Effect of Inducible Co-Stimulatory Molecule siRNA in Cerebral Infarction Rat Models.
CONCLUSIONS ICOS siRNA can protect brain tissues from ischemia injuries after cerebral infarction, improve limb movement and coordination, lower the mortality rate of rats, and inhibit T cell-induced cytokines. These results collectively suggest the potential treatment efficacy of ICOS siRNA against cerebral infarction. PMID: 26436531 [PubMed - as supplied by publisher]
Source: Medical Science Monitor - October 6, 2015 Category: Research Tags: Med Sci Monit Source Type: research

Cholesterol Grafted Cationic Lipopolymers: Potential siRNA Carriers for Selective Chronic Myeloid Leukemia (CML) Therapy.
This article is protected by copyright. All rights reserved. PMID: 31714657 [PubMed - as supplied by publisher]
Source: Biomed Res - November 11, 2019 Category: Research Authors: Remant KC, Thapa B, Valencia-Serna J, Domun SS, Dimitroff C, Jiang X, Uludağ H Tags: J Biomed Mater Res A Source Type: research

Effects of siRNA-Mediated Knockdown of HDAC1 on the Biological Behavior of Esophageal Carcinoma Cell Lines.
CONCLUSIONS The siRNA-mediated HDAC1 knockdown significantly inhibited the proliferation, migration and invasion of TE-1 cells probably by regulating the expression of cell cycle- and EMT-related proteins. PMID: 27086779 [PubMed - in process]
Source: Medical Science Monitor - April 20, 2016 Category: Research Tags: Med Sci Monit Source Type: research

Molecular basis for improved gene silencing by Dicer substrate interfering RNA compared with other siRNA variants
The canonical exogenous trigger of RNA interference (RNAi) in mammals is small interfering RNA (siRNA). One promising application of RNAi is siRNA-based therapeutics, and therefore the optimization of siRNA efficacy is an important consideration. To reduce unfavorable properties of canonical 21mer siRNAs, structural and chemical variations to canonical siRNA have been reported. Several of these siRNA variants demonstrate increased potency in downstream readout-based assays, but the molecular mechanism underlying the increased potency is not clear. Here, we tested the performance of canonical siRNAs and several sequence-mat...
Source: Nucleic Acids Research - June 26, 2013 Category: Research Authors: Snead, N. M., Wu, X., Li, A., Cui, Q., Sakurai, K., Burnett, J. C., Rossi, J. J. Tags: RNA Source Type: research

siPools: highly complex but accurately defined siRNA pools eliminate off-target effects
Short interfering RNAs (siRNAs) are widely used as tool for gene inactivation in basic research and therapeutic applications. One of the major shortcomings of siRNA experiments are sequence-specific off-target effects. Such effects are largely unpredictable because siRNAs can affect partially complementary sequences and function like microRNAs (miRNAs), which inhibit gene expression on mRNA stability or translational levels. Here we demonstrate that novel, enzymatically generated siRNA pools—referred to as siPools—containing up to 60 accurately defined siRNAs eliminate off-target effects. This is achieved by th...
Source: Nucleic Acids Research - July 3, 2014 Category: Research Authors: Hannus, M., Beitzinger, M., Engelmann, J. C., Weickert, M.-T., Spang, R., Hannus, S., Meister, G. Tags: RNA Source Type: research

Systematic evaluation of antibody-mediated siRNA delivery using an industrial platform of THIOMAB-siRNA conjugates
We report that such coupling generates monomeric antibody–siRNA conjugates (ARCs) that retain antibody and siRNA activities. To broadly assess this technology, we generated a battery of THIOMABs against seven targets that use multiple internalization routes, enabling systematic manipulation of multiple parameters that impact delivery. We identify ARCs that induce targeted silencing in vitro and extend tests to target prostate carcinoma cells following systemic administration in mouse models. However, optimal silencing was restricted to specific conditions and only observed using a subset of ARCs. Trafficking studies ...
Source: Nucleic Acids Research - January 23, 2015 Category: Research Authors: Cuellar, T. L., Barnes, D., Nelson, C., Tanguay, J., Yu, S.-F., Wen, X., Scales, S. J., Gesch, J., Davis, D., van Brabant Smith, A., Leake, D., Vandlen, R., Siebel, C. W. Tags: RNA Source Type: research