Activation of Uterine Smad3 Pathway Is Crucial for Embryo Implantation
In this study, we measured the expre ssion of TGF-β1, TGF-β receptor type I (TβRI), Smad3 and p-Smad3 in the endometrium of mice and observed their elevation on day 4, 5 and 6 of pseudopregnancy. Then we administrated a specific Smad3 inhibitor (Sis3) into the uterine cavity of mice on day 3 of pregnancy. The results showed a reduct ion in insulin-like growth factor-1 (IGFBP-1) expression and the decreased number of implanted embryo after the administration. In addition, Sis3 was found to reduce the IGFBP-1 secretion in decidualized endometrial stromal cells. Taken all together, our findings demonstrated tha...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Influence of Acupuncture on Autonomic Balance in Adult Tinnitus Patients: An Exploratory Study
SummaryAcupuncture is an alternative therapy for tinnitus in clinical practice. The mechanism by which acupuncture can alleviate tinnitus is still unknown. Autonomic nervous system was reported to be responsible for tinnitus. The aim of this study was to explore the effect of acupuncture on autonomic balance in adult tinnitus patients. Thirty patients were randomly assigned into either the deep acupuncture (DA) group or the shallow acupuncture (SA) group. Each patient received 6 acupuncture sessions (a –f phase) over three weeks. Measures of heart rate variability and Tinnitus Handicap Inventory (THI) were obtained a...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Dectin-1 rs3901533 and rs7309123 Polymorphisms Increase Susceptibility to Pulmonary Invasive Fungal Disease in Patients with Acute Myeloid Leukemia from a Chinese Han Population
In conclusion, twoDectin-1 SNPs (rs3901533 and rs7309123) are associated with increased susceptibility to pulmonary IFD in AML patients in a Chinese Han population. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Risk Factors and Management of Postoperative Pancreatic Fistula Following Pancreaticoduodenectomy: Single-center Experience
In conclusion, soft pancreatic texture is an independent risk factor for PF. Surgeons should be well aware of this risk factor when performing a PD. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Adverse Effects of Exposure to Fine Particulate Matters and Ozone on Gestational Hypertension
SummaryGestational hypertension (GH) is a common complication during pregnancy. GH is regarded as a potential public health challenge for pregnant women and infants. Limited evidence has linked ambient air pollution to an increased GH risk. However, most of the studies were conducted in developed countries, with inconsistent results obtained. The present study was performed to explore whether exposure to particulate matters with an aerodynamic diameter< 2.5 (PM2.5) and ozone (O3) was related to elevated odds of GH in a Chinese population. This population-based cohort study involved 38 115 pregnant women in Wuhan, Ch...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Expression of Stanniocalcin 2 in Breast Cancer and Its Clinical Significance
In conclusion, the abnormal overexpression of STC2 gene may play a role in the development and progression of breast cancer, and it can be used as an independent metastasis and prognostic factor of breast cancer. In addition, STC2 gene probably promotes the development and metastasis of breast cancer by interacting with estrogen and ER, and it may become a new direction for breast cancer endocrine therapy. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Protective Effects of α-Lipoic Acid on Vascular Oxidative Stress in Rats with Hyperuricemia
SummaryThe aim of the present study was to observe the protective effects of α-lipoic acid (ALA) on vascular injury in rats with hyperuricemia (HUA). The ALA treatment groups (10, 30 and 90 mg/kg, respectively) were administered with ALA via gavage for 2 weeks. Subsequently, the levels of blood urea nitrogen (BUN), creatinine (CREA), uric acid (UA), total cholesterol (TC), high density lipoprotein-C (HDL-C) and low density lipoprotein-C (LDL-C) were measured; the activities of glutathione peroxidase (GSH-Px), catalase (CAT), malonaldehyde (MDA), superoxide dismutase (SOD) and xanthine oxidase (XOD) were also determin...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Voltage-gated Sodium Channels and Blockers: An Overview and Where Will They Go?
SummaryVoltage-gated sodium (Nav) channels are critical players in the generation and propagation of action potentials by triggering membrane depolarization. Mutations in Nav channels are associated with a variety of channelopathies, which makes them relevant targets for pharmaceutical intervention. So far, the cryoelectron microscopic structure of the human Nav1.2, Nav1.4, and Nav1.7 has been reported, which sheds light on the molecular basis of functional mechanism of Nav channels and provides a path toward structure-based drug discovery. In this review, we focus on the recent advances in the structure, molecular mechani...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Licorice Extracts Attenuate Nephrotoxicity Induced by Brucine Through Suppression of Mitochondria Apoptotic Pathway and STAT3 Activation
This study aimed to evaluate the protective effect of licorice on brucine-induced nephrotoxicity. Sprague Dawley rats were administered with brucine intraperitoneally for 7 consecutive days with or without treatment with licorice. The content of blood urea nitrogen and creatinine in serum, the activities of superoxide dismutase and content of glutathione, malonaldehyde in kidney tissue were detected. Hematoxylin-eosin staining was employed to observe the histopathological changes of kidney. The expression and phosphorylation levels of protein were evaluated by Western blotting and immunohistochemical analysis. The results ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Short-lived AIM2 Inflammasome Activation Relates to Chronic MCMV Infection in BALB/c Mice
In conclusion, the activation of AIM2 inflammasome in BALB/c mice was short-lived, which is quite possibly related to the chronicity of MCMV infection. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Gut Microbiota Reconstruction Following Host Infection with Blood-stage Plasmodium berghei ANKA Strain in a Murine Model
SummaryMalaria remains a global health problem. The relationship betweenPlasmodium spp. and the gut microbiota as well as the impact ofPlasmodium spp. on the gut microbiota in vertebrate hosts is unclear. The aim of the current study was to evaluate the effect of blood-stagePlasmodium parasites on the gut microbiota of mice. The gut microbiota was analyzed by 16S rRNA sequencing and bioinformatic analyses at three stages. The gut microbiota changed during the three phases: the healthy stage, the infection stage, and the cure stage (on the 9th day after malarial elimination). Moreover, the gut microbiota of these infected a...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Experimental Study and Preliminary Clinical Application of Mini-invasive Percutaneous Internal Screw Fixation for Scaphoid Fracture under the Guidance of a 3D-printed Guide Plate
This study explored the feasibility of employing computer-aided design (CAD) and 3 dimensional (3D)-printed personalized guide plate for the mini-invasive percutaneous internal screw fixation of fractured scaphoid. The study consisted of two parts: (1) experimentation on upper limbs from corpses and (2) preliminary clinical application. Corpse experiments involved upper limbs of 6 adult corpses. The specimens of upper limbs were subjected to plain CT scan. Then the CT data were input into computer to conduct 3D reconstruction of wrist region. The direction and depth of the guide wire and screw were designed on the basis of...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Glycerin Monostearate Aggravates Male Reproductive Toxicity Caused by Di(2-ethylhexyl) Phthalate in Rats
SummaryHuman beings are increasingly exposed to phthalates, which are a group of chemicals used to make plastics more flexible and harder to break, and simultaneously ingesting abundant food emulsifiers via daily diet. The purpose of this study was to investigate the effect of the food emulsifier glycerin monostearate (GMS) on male reproductive toxicity caused by di(2-ethylhexyl) phthalate (DEHP, one of the phthalates) and explore the underlying mechanism. Thirty male Sprague-Dawley rats were randomly divided into control group, DEHP group and DEHP+GMS group. Rats in the DEHP group and DEHP+GMS group were orally administer...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

The Role of Endolymphatic Hydrops in Patients with Pantonal Idiopathic Sudden Sensorineural Hearing Loss: A Cause or Secondary Reaction
In conclusion, there is EH in the inner ear of patients with pantonal ISSNHL; EH is not related to vertigo, a concomitant symptom of ISSNHL, and the prognosis of this condition. The presence of EH may be a secondary reaction following the impairment of the inner ears with pantonal ISSNHL. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Knockout of MicroRNA-155 Ameliorates the Th17/Th9 Immune Response and Promotes Wound Healing
In this study, qPCR analyses were performed to determine which miRNAs play an important role in wound healing. Next, an experiment in a model of wound healing was performed, and histology, mRNA expression and T-cell subpopulations in wound tissue were analyzed. The accelerated experiments were performed by local injection of either rIL-17A and/or rIL-9 after wound healing.In vitro, the differentiation of Th17/Th9 in miR-155+/+ or miR-155−/− mice was investigated, and the target genes of miR155 were analyzed. From our findings, miR-155−/− in mice promoted wound healing and weakened T cell-mediated in...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Shenqi Fuzheng Injection Ameliorates Radiation-induced Brain Injury
This study examined the brain-protective effect of SFI on patients after cranial radiation. Lung cancer patients with brain metastasis were randomly assigned to two groups. The SFI group received cranial radiation in combination with SFI. The control group received cranial radiation alone. The changes in cognitive function were evaluated pre- and post-radiation against the Mini-Mental State Exam (MMSE), Montreal Cognitive Assessement (MoCA), Zung Self-Rating Depression Scale (SDS) and Zung Self-Rating Anxiety Scale (SAS). The changes in inflammatory factors, such as TGF- β1, TNF-α and IL-10, were also detected b...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Diwu Yanggan Modulates the Wnt/ β-catenin Pathway and Inhibits Liver Carcinogenesis Signaling in 2-AAF/PH Model Rats
SummaryThe activation of the Wnt/ β-catenin signaling pathway in oval cells after liver injury is implicated in hepatocarcinogenesis. Diwu Yanggan capsule is a Chinese herbal medicine that has been used for treating liver disorder. The present study aimed to examine the mechanism by which Diwu Yanggan inhibits liver carcinogenesis, and the involvement of the Wnt/β-catenin signaling pathway. Diwu Yanggan capsule was administered to 2-acetaminofluorene/partial hepatectomy (2-AAF/PH) rats, a murine model of liver injury. The biomarkers of oval cells and key proteins in the Wnt/β-catenin signaling pathway were a...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Hydrogen Sulfide Attenuates High Glucose-induced Myocardial Injury in Rat Cardiomyocytes by Suppressing Wnt/beta-catenin Pathway
SummaryDiabetic cardiomyopathy (DCM) is one of the major heart complications of diabetic patients. Hydrogen sulfide (H2S) is now recognized as an important signaling molecule and has been shown to attenuate the development of diabetic cardiomyopathy. However, the underlying mechanisms linking H2S and the development of DCM have not been fully elucidated. In the present study, we therefore sought to explore the role and mechanism of H2S in the pathogenesis of DCM by establishing high glucose-induced injury model in neonatal rat cardiomyocytes (NRCMs) and H9c2 cells. Using cystathionine gamma-lyase (CSE) overexpression and C...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Patellofemoral MRI Alterations Following Single Bundle ACL Reconstruction with Hamstring Autografts Are Associated with Quadriceps Femoris Atrophy
This study was designed to define the relationship between the unstability of patellofemoral joint and quadriceps femoris atrophy. Twenty patients underwent MRI scan before ACL reconstruction and every two weeks after surgery, until 12 weeks. The merchant ’s patellar congruence angle, lateral inclination angle, and quadriceps femoris muscle cross-sectional area were measured and the relationship between the changes of angles and the ratio of quadriceps femoris atrophy was studied by multiple regression analysis. Significant quadriceps femoris atrop hy was observed after ACL reconstruction during the follow-up period ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

CAR T Cell Therapy for Hematological Malignancies
SummaryAs a rapidly progressing field in oncology, the adoptive transfer of T cells that have been genetically modified with chimeric antigen receptors (CARs) has shown striking efficacy in the management of hematological malignancies and has been reported in a number of clinical trials. Of note, CAR T cell therapy has shown extraordinary potential, especially in relapsed/refractory patients. However, there are still challenges regarding the further development of this strategy, spanning from engineering and manufacturing issues, to limited applications, to accompanying toxicities. In this review, we will summarize the gen...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - December 1, 2019 Category: Research Source Type: research

Identification of Factors Influencing Out-of-county Hospitalizations in the New Cooperative Medical Scheme
SummaryThroughout the duration of the New Cooperative Medical Scheme (NCMS), it was found that an increasing number of rural patients were seeking out-of-county medical treatment, which posed a great burden on the NCMS fund. Our study was conducted to examine the prevalence of out-of-county hospitalizations and its related factors, and to provide a scientific basis for follow-up health insurance policies. A total of 215 counties in central and western China from 2008 to 2016 were selected. The total out-of-county hospitalization rate in nine years was 16.95%, which increased from 12.37% in 2008 to 19.21% in 2016 with an av...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Association between KITLG Gene Polymorphisms and Testicular Germ Cell Tumors: A Systematic Review and Meta-analysis
SummaryIt has been reported that c-KIT ligand (KITLG) gene polymorphisms may be associated with testicular germ cell tumors (TGCT). Owing to mixed and inconclusive results, we conducted a systematic review and meta-analysis to summarize and clarify this association. A systematic search of studies on the association between KITLG gene polymorphisms and TGCT susceptibility was conducted in databases. Odds ratios and 95% confidence intervals were used to pool the effect size. Six articles were included in our systematic review and meta-analysis. Compared with adenine (A), KITLG rs995030 guanine (G) might be associated with in...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Intense Pulsed Light Therapy for Patients with Meibomian Gland Dysfunction and Ocular Demodex Infestation
SummaryTo observe the clinical changes of meibomian gland dysfunctipn (MGD) and ocularDemodex infestation after intense pulsed light (IPL) treatment to further examine the mechanism of IPL treating patients with MGD and ocularDemodex infestation. The medical records of 25 patients (49 eyes) with MGD treated with IPL, were retrospectively examined to determine outcomes. Associated ocular-surface parameters (ocular surface disease index, OSDI; lipid layer thickness, LLT; noninvasive first breakup time, NIF-BUT; noninvasive average breakup time, NIAvg-BUT; tear film breakup area, TBUA; Schirmer I Test, SIT; corneal fluorescei...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Visit-to-visit Systolic Blood Pressure Variability and Stroke Risk: A Systematic Review and Meta-analysis
SummaryVisit-to-visit variability in systolic blood pressure (SBP) may have an important additional role in increasing the risk of vascular complications, including stroke. We conducted a meta-analysis to assess the relationship between visit-to-visit SBP variability (SBPV) and stroke risk. PubMed, EMBASE, and the Cochrane library databases were searched for cohort studies with data on visit-to-visit SBPV and stroke risk. Studies that reported adjusted relative risks (RRs) with 95% CIs of stroke associated with SBPV were included. Fourteen cohort studies met the inclusion criteria and were included in our meta-analysis. Af...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Coronary CT Angiography Using Low Iodine Delivery Rate and Tube Voltage Determined by Body Mass Index: Superiority in Clinical Practice
SummaryTo explore the feasibility and superiority of iodine delivery rate (IDR) and tube voltage determined by patients ’ body mass index (BMI) in coronary CT angiography (CCTA), a total of 1567 patients undertaking CCTA during Feb. and Dec. 2016 were enrolled and divided into two groups. In the control group, the IDR and tube voltage were fixed, while in the experimental group, the IDR and tube voltage were determ ined by patients’ BMI. The volume of iodinated contrast media (ICM), extravasation rate, extravasation volume, extravasation recovery interval, incidence rate of adverse reactions, effective dose (ED...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Double-edge Role of B Cells in Tumor Immunity: Potential Molecular Mechanism
SummaryB cells are a heterogeneous population, which have distinct functions of antigen presentation, activating T cells, and secreting antibodies, cytokines as well as protease. It is supposed that the balance among these B cells subpopulation (resting B cells, activated B cells, Bregs, and other differentiated B cells) will determine the ultimate role of B cells in tumor immunity. There has been increasing evidence supporting opposite roles of B cells in tumor immunity, though there are no general acceptable phenotypes for them. Recent years, a new designated subset of B cells identified as Bregs has emerged from immunos...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Short-term Outcomes of Laparoscopic vs. Open Hepatectomy for Primary Hepatocellular Carcinoma: A Prospective Comparative Study
This study retrospectively analyzed the demographic data and short-term outcomes of 81 patients who underwent LH or OH for the primary treatment of PHC between Oct. 2017 and May 2018 at Union Hospital, Tongji Medical College, Huazhong University of Science and Technology (China). A total of 81 PHC patients who received major liver resection were enrolled. There were 38 (47%) patients in the LH group and 43 (53%) patients in the OH group. The operative time was significantly longer (373.53 ±173.38vs. 225.43 ±55.08,P
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Superinfective Hepatitis E Virus Infection Aggravates Hepatocytes Injury in Chronic Hepatitis B
SummaryHepatitis E virus (HEV) infection is a major cause of morbidity in endemic areas. Its consequences among chronic hepatitis B (CHB) patients have been under-reported. The aim of this study was to assess the impact of superinfective HEV infection (acute and past) on virological and clinical features of patients with CHB infection. Clinical, biochemical, virological and immunological data of 153 CHB patients including 98 with hepatitis B virus (HBV) monoinfection and 55 with HBV-HEV superinfection with both HEV and HBV infection was retrospectively investigated and analyzed in this study conducted in Wuhan, China. An o...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Clinical Features and Microvascular Complications Risk Factors of Early-onset Type 2 Diabetes Mellitus
SummaryThe aim of this research was to study the clinical features and microvascular complications risk factors of early-onset type 2 diabetes mellitus (T2DM). We analyzed the clinical data from 1421 T2DM inpatients at Wuhan Union Hospital. Subjects were divided into early-onset T2DM group (diagnostic age40 years). All subjects underwent a standardized assessment of microvascular complications. Data were compared with independent-samplest test or Chi-square test. Multiple logistic regression was used to determine the risk factors of microvascular complications. Patients with early-onset T2DM were more inclined to have a lo...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Role of Mutant TBP in Regulation of Myogenesis on Muscle Satellite Cells
SummaryIn polyglutamine (PolyQ) diseases, mutant proteins cause not only neurological problems but also peripheral tissue abnormalities. Among all systemic damages, skeletal muscle dystrophy is the severest. Previously by studying knock-in (KI) mouse models of spinal cerebellar ataxia 17 (SCA17), it was found that mutant TATA box binding protein (TBP) decreases its interaction with myogenic differentiation antigen, thus reducing the expression of skeletal muscle structural proteins and resulting in muscle degeneration. In this paper, the role of mutant TBP in myogenesis was investigated. Single myofibers were isolated from...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Analysis of Ornidazole Injection in Clinical Use at Post-marketing Stage by Centralized Hospital Monitoring System
This study aims to analyze the clinical use of ornidazole injection at the post-marketing stage by centralized hospital monitoring system method, and investigate its widespread use in patients, in order to regulate and guide the rational drug use, improve the drug specificity and provide a basis for drug therapy. The study adopts a prospective, multi-center, large sample size, centralized hospital monitoring system. We selected five leading hospitals in Hubei province, and observed the inpatients who received the ornidazole injection from July 1, 2015 to October 31, 2015. The basic information of patients was recorded, as ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Long Noncoding RNA UCA1 Overexpression Is Associated with Poor Prognosis in Digestive System Malignancies: A Meta-analysis
SummaryLong noncoding RNA (lncRNA) urothelial carcinoma associated 1 (UCA1) has been reported to be highly expressed in many kinds of cancers. This meta-analysis summarized its potential prognostic value in digestive system malignancies. A meta-analysis was performed through a comprehensive search in PubMed, EMBASE, the Cochrane Library, Web of Science and Chinese National Knowledge Infrastructure (CNKI) for suitable articles on the prognostic impact of UCA1 in digestive system malignancies from inception to June 27, 2019. Pooled hazard ratios (HRs) with 95% confidence interval (95%CI) were calculated to summarize the effe...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Evaluation of Ultrasound-guided Genitofemoral Nerve Block Combined with Ilioinguinal/iliohypogastric Nerve Block during Inguinal Hernia Repair in the Elderly
SummaryTo evaluate the anesthetic effect of ultrasound-guided (USG) ilioinguinal/iliohypogastric nerve (II/IHN) block combined with genital branch of genitofemoral nerve (GFN) block in the elderly undergoing inguinal hernia repair, 54 old patients (aged 60 –96years, ASA I–III) with indirect hernia were enrolled and scheduled for unilateral tension-free herniorrhaphy. Patients were grouped randomly to receive either USG II/IHN plus GFN block (Group G) or USG II/IHN block alone (Group I). The intraoperative visual analogue scale (VAS) scores were re corded at skin incision, at spermatic cord/round ligament tracti...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Establishment of Rabbit Liver VX2 Tumor Model Using Percutaneous Puncture Inoculation of Tumor Fragment Guided and Evaluated by Ultrasonography
SummaryThe aim of the present study is to evaluate a method of establishing model of rabbit liver VX2 tumor using percutaneous puncture inoculation of tumor fragment guided by ultrasonography. VX2 tumor fragments were implanted into the liver of 13 New Zealand white rabbits flushed by 1 mL normal saline through percutaneous puncture needle guided by ultrasonography. Conventional ultrasonography and contrast-enhanced ultrasonography (CEUS) were performed 14 days after inoculation, and then the rabbits were sacrificed and pathologically examined. The success rate of inoculation was 100%. The average size of liver VX2 tumor w...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

A Comprehensive Understanding of Dietary Effects on C. elegans Physiology
SummaryDiet has been shown to play an important role in human physiology. It is a predominant exogenous factor regulating the composition of gut microbiota, and dietary intervention holds promise for treatment of diseases such as obesity, type 2 diabetes, and malnutrition. Furthermore, it was reported that diet has significant effects on physiological processes ofC. elegans, including reproduction, fat storage, and aging. To reveal novel signaling pathways responsive to different diets,C. elegans and its bacterial diet were used as an interspecies model system to mimic the interaction between host and gut microbiota. Most ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Curcumin Recovers Intracellular Lipid Droplet Formation Through Increasing Perilipin 5 Gene Expression in Activated Hepatic Stellate Cells In Vitro
This study aims at evaluating the effect of curcumin on lipid accumulation in HSCs and hepatocytes, and further elucidating the underlying mechanisms. Now we showed that curcumin increased LD formation in activated HSCs and stimulated the expression of sterol regulatory element-binding protein and fatty acid synthase, and reduced the expression of adipose triglyceride lipase. Exogenous perilin5 expression in primary HSCs promoted LD formation. Perilipin 5 siRNA eliminated curcumin-induced LD formation in HSCs. The se results suggest that curcumin recovers LD formation and lipid accumulation in activated HSCs by increasing ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Body Weight Misperception and Weight Disorders among Chinese Children and Adolescents: A Latent Class Analysis
SummaryBody misperception plays an important role in the development of weight and dietary disorders among children and adolescents. A school-based health promotion program (2014 –2015) was conducted to promote the school health education and improve the teenagers’ physical health among Chinese children and adolescents. Based on this program, we intended to examine weight status and weight misperception among Chinese children and adolescents and to explore the relationsh ip between weight misperception and lifestyle behaviors. A total of 10 708 Chinese children and adolescents in 3rd and 7th grade from Shandong...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Tamsulosin Monotherapy Is Effective in Reducing Ureteral Stent-related Symptoms: A Meta-analysis of Randomized Controlled Studies
This study aimed to evaluate the effectiveness of tamsulosin monotherapy for the treatment of ureteral stent-related symptoms (SRSs) and compare it with that of solifenacin monotherapy and combined therapy of tamsulosin and silifenacin. Randomized controlled trials (RCTs), which evaluated the effectiveness of tamsulosin for the treatment of SRSs, were searched from the databases PubMed, EMBASE and the Cochrane Library published up to November 2018. Eight RCTs involving 1087 participants were finally included in this meta-analysis. The results showed that tamsulosin monotherapy could significantly decrease the urinary sympt...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Influence of Collective Esophageal Speech Training on Self-efficacy in Chinese Laryngectomees: A Pretest-posttest Group Study
In this study, 28 patients and 30 family members were included. The participants received information about training via telephone or a WeChat group. Collective esophageal speech training was used to educate laryngectomees on esophageal speech. Before and after collective esophageal speech training, all participants completed the General Self-Efficacy Scale (GSES) to assess their perceptions on self-efficacy. Through the training, laryngectomees recovered their speech. After the training, the self-efficacy scores of laryngectomees were higher than those before the training, with significant differences noted (T
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF- β1/Smads Signaling Pathway
SummaryAlthough several studies confirmed that berberine may attenuate airway inflammation in mice with chronic obstructive pulmonary disease (COPD), its underlying mechanisms were not clear until now. We aimed to establish an experiment mouse model for COPD and to investigate the effects of berberine on airway inflammation and its possible mechanism in COPD model mice induced by cigarette smoke extract (CSE). Twenty SPF C57BL/6 mice were randomly divided into PBS control group, COPD model group, low-dose berberine group and high-dose berberine group, 5 mice in each group. The neutrophils and macrophages were examined by W...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - October 1, 2019 Category: Research Source Type: research

Implications of Necroptosis for Cardiovascular Diseases
SummaryNecroptosis is a non-apoptotic programmed cell death pathway, which causes necrosis-like morphologic changes and triggers inflammation in the surrounding tissues. Accumulating evidence has demonstrated that necroptosis is involved in a number of pathological processes that lead to cardiovascular diseases. However, the exact molecular pathways linking them remain unknown. Herein, this review summarizes the necroptosis-related pathways involved in the development of various cardiovascular diseases, including atherosclerosis, cardiac ischemia-reperfusion injury, cardiac hypertrophy, dilated cardiomyopathy and myocardia...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Development and Validation of a Simplified Chinese Version of the Assessment Tool for Students ’ Perceptions of Medical Professionalism
This study aimed to validate an effective assessment tool for the measurement of medical students’ perceptions of medical professionalism in mainland China. The cross-sectional survey was co nducted in three medical colleges in Guangdong, China. Of the 2103 eligible medical students, 1976 responded, and 1856 questionnaires were deemed valid. Students from clinical medicine in these three medical colleges were randomly selected by cluster sampling. First, a Simplified Chinese Version que stionnaire to measure Student’s Perception of Medical Professionalism (SCV-SPMP) was constructed. Second, questionnaires from ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Bcl6 Suppresses Cardiac Fibroblast Activation and Function via Directly Binding to Smad4
In conclusion, Bcl6 may negatively regulate cardiac fibroblast activation and function by directly binding to smad4. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

A Heterologous Fibrin Glue Enhances the Closure Effect of Surgical Suture on the Repair of Annulus Fibrous Defect in a Sheep Model
SummaryImproving the closure effect of surgical suture for repair of annulus fibrosus defects remains an unsolved problem. A new type of porcine fibrin glue was reported for the repair of annulus fibrous defects in sheep models in this study. Continuous axial loading test showed that this glue could effectively improve the closure effect of surgical suture for annulus fibrous defect. Magnetic resonance imaging (MRI) of the lumbar spine confirmed that, compared with non-fibrin glue treated intervertebral discs, it contributed to preservation of the nucleus pulposus and maintained the physiological hydration of the intervert...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Prevalence and Determinants of High-risk HPV Infection among 11549 Women from an Opportunistic Screening in Hubei Province
This study confirms that hrHPV infection was associated with age, marital status, sympt oms of intercourse bleeding, history of sexually transmitted infections, and sex-related behaviors. Above all, this study provides a baseline database prior to obtaining vaccinations for dynamic tracking of the changes in hrHPV prevalence. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Thyroid Nodules in Type 2 Diabetes Mellitus
SummaryThe present analysis aims to investigate the prevalence of thyroid nodules in type 2 diabetes mellitus (T2DM) population. We searched PubMed, EMBASE, and Web of Science from inception to the March 1, 2018. The studies were selected to estimate the prevalence of thyroid nodules in T2DM subjects and to compare the prevalence of thyroid nodules in different glucose tolerance status. The random effects model was used, and the outcome was presented as a pooled prevalence proportion with 95% confidence interval (95% CI) or a summary odds ratio (OR) with 95% CI. In the end, 9 studies met the inclusion criteria and were inc...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Congenital Cystic Adenomatoid Malformation Volume Ratio in Prenatal Assessment of Prognosis of Fetal Pulmonary Sequestrations
This study aimed to evaluate the prognosis of pulmonary sequestration (PS) by measuring congenital cystic adenomatoid malformation volume ratio (CVR) value in fetal congenital PS. The fetal CVR in 49 cases of fetal PS diagnosed by prenatal ultrasound in Xiangyang No. 1 People ’s Hospital from March 2010 to June 2017 were measured, and the clinical outcomes were observed. According to the prenatal ultrasound CVR value, 49 fetuses diagnosed with PS were divided into 2 groups: group 1 with CVR≥1.26, and group 2 with CVR
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Treatment of Post-traumatic Pediatric Ankle Varus Deformity with Physeal Bar Resection and Hemi-Epiphysiodesis
This study evaluates the treatment of post-traumatic pediatric ankle varus deformity using physeal bar resection and hemi-epiphysiodesis with the assistance of two operative methods. Forty-five patients presenting with a distal tibial medial physeal bridge as well as ankle varus deformity following traumatic ankle physeal injury between 2009 and 2017 were followed. These patients were treated with physeal bar resection and hemi-epiphysiodesis, with the assistance of either fluoroscopy (10 cases) or intraoperative three-dimensional navigation (35 cases). Of the 45 cases, the median age was 9.0 years (range: 3 –14 year...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway
SummaryActivation of macrophages is a key event for the pathogenesis of various inflammatory diseases. Notch signaling pathway recently has been found to be a critical pathway in the activation of proinflammatory macrophages. Salidroside (Sal), one of main bioactive components inRhodiola crenulata (Hook. F. et Thoms)H. ohba, reportedly possesses anti-inflammatory activity and ameliorates inflammation in alcohol-induced hepatic injury. However, whether Sal regulates the activation of proinflammatory macrophages through Notch signaling pathway remains unknown. The present study investigated the effects of Sal on macrophage a...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research

Dynamic Variation of RAS on Silicotic Fibrosis Pathogenesis in Rats
SummaryThe dynamic variation of renin-angiotensin system (RAS) in silicosis remains unclear. Seventy Wistar rats were divided into 7 groups including control group, silicosis groups (inhaling SiO2 for 2, 4, 8, 16 and 24 weeks, respectively) and Captopril (Cap) group. Rat lung primary fibroblasts were divided into control group, SiO2-stimulated group (0, 0.5, 1, 3, 6, 12, 24 and 48 h) and Cap group. The silicotic nodules were formed and collagens were deposited gradually in silicosis group observed by haematoxylin and eosin (HE) staining and Van Gieson (VG) staining. Cap relieved the lung fibrosis and collagen deposition. I...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - July 25, 2019 Category: Research Source Type: research