Recent Developments in the Treatment of Wet Age-related Macular Degeneration
This article focuses on the latest treatment approaches to neovascular AMD. An extensive literature review was performed in order to illustrate the effectiveness of current and future anti-VEGF agents as well as the landmark clinical studies that have been carried out to establish these drugs as a gold standard in the therapy of wet AMD. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - September 27, 2020 Category: Research Source Type: research

Identification of Hub Genes and Key Pathways Associated with Peripheral T-cell Lymphoma
In this study, we chose the gene expression profile of GSE6338 from the Gene Expression Omnibus (GEO) database to identify hub genes and key pathways and explore possible molecular pathogenesis of PTCL by bioinformatic analysis. Differentially expressed genes (DEGs) between PTCL and normal T cells were selected using GEO2R tool. Gene ontology (GO) analysis and Kyoto Encyclopedia of Gene and Genome (KEGG) pathway analysis were performed using Database for Annotation, Visualization and Integrated Discovery (DAVID). Moreover, the Search Tool for the Retrieval of Interacting Genes (STRING) and Molecular Complex Detection (MCOD...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - September 27, 2020 Category: Research Source Type: research

Shenkang VII Recipe Attenuates Unilateral Ureteral Obstruction-induced Renal Fibrosis via TGF- β/Smad, NF-κB and SHH Signaling Pathway
This study aimed to explore the protective effects of the traditional Chinese Medicine formula Shenkang VII recipe (SK-7) on renal fibrosis and the mechanisms. Renal fibrosis was induced by unilateral ureteral obstruction (UUO) in rats. The rats were then divided into 5 groups: control group (Sham operation), UUO model group, UUO model plus low to high doses of SK-7 (0.5, 1.0, or 2.0 g/kg/day, for 14 days) groups. The animals were sacrificed on the 7th or 14th day. Kidney tissues were collected for histopathological examinations (hematoxylin and eosin and Masson ’s trichrome staining). Immunohistochemistry was used t...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - September 27, 2020 Category: Research Source Type: research

Emergency Biosafety Management Practice in Laboratory of Shelter Hospital
SummaryAt the end of 2019, the novel coronavirus infection outbroke in Wuhan, Hubei Province. On Feb. 2, 2020, Wuhan, as the worst-hit region, began to build “shelter hospital” rapidly to treat patients with mild illness. The shelter hospital has multiple functions such as emergency treatment, surgical treatment and clinical test, which can adapt to emergency medical rescue tasks. Based on the characteristics that shelter hospital only treats patient s with mild illness, tests of shelter laboratory, including coronavirus nucleic acid detection, IgM/IgG antibody serology detection, monitoring and auxiliary diagn...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - September 27, 2020 Category: Research Source Type: research

High-quality Cleavage Embryo versus Low-quality Blastocyst in Frozen-thawed Cycles: Comparison of Clinical Outcomes
This study compared the clinical outcomes of the frozen-thawed cycles of high-quality cleavage embryos with low-quality blastocysts to provide a reference for the choice of frozen-thawed embryo transfer schemes and to improve clinical pregnancy rates. A retrospective analysis was performed on the clinical data of patients undergoing frozen-thawed embryo transfer at the Reproductive Medicine Center of Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology from 2016 to 2017. In total, 845 cases were divided into a high-quality cleavage embryo group (group A) and a low-quality blastocyst gr...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - September 27, 2020 Category: Research Source Type: research

Bone Microthrombus Promotes Bone Loss in Iron Accumulation Rats
SummaryIn the present study, we investigated the changes of the coagulation state, bone microthrombus, microvascular bed and bone density levels in iron accumulation rats. Meanwhile, the effect of anticoagulation therapy on bone mineral density was further investigated. We established two groups: a control (Ctrl) group and an iron intervention (FAC) group. Changes in coagulation function, peripheral blood cell counts, bone microthrombus, bone vessels and bone mineral density were compared between the two groups. We designed the non-treatment group and treatment group to study the changes of bone mineral density by preventi...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - September 27, 2020 Category: Research Source Type: research

Respiratory Support in Severely or Critically Ill ICU Patients With COVID-19 in Wuhan, China
SummaryThis case series aimed to describe the clinical characteristics of severely or critically ill patients with COVID-19 and compare the clinical characteristics of patients who received invasive respiratory support with those of patients who received noninvasive respiratory support. We included all confirmed severe or critical illness cases of COVID-19 admitted to the Intensive Care Unit (ICU) of Zhongnan Hospital of Wuhan University, a COVID-19-designated hospital, from January 8 to March 12, 2020. Cases were analyzed for epidemiological, demographic, clinical, APACHE II, SOFA, radiological features and laboratory dat...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Accumulated Clinical Experiences from Successful Treatment of 1377 Severe and Critically Ill COVID-19 Cases
SummaryIn late December 2019, COVID-19 was firstly recognized in Wuhan, China and spread rapidly to all of the provinces of China. The West Campus of Wuhan Union Hospital, the designated hospital to admit and treat the severe and critically ill COVID-19 cases, has treated a large number of such patients with great success and obtained lots of valuable experiences based on the Chinese guideline (V7.0). To standardize and share the treatment procedures of severe and critically ill cases, Wuhan Union Hospital has established a working group and formulated an operational recommendation, including the monitoring, early warning ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Management and Reconfiguration of a Radiology Department under the Threat of Coronavirus Disease 2019: Experience from Wuhan
This article included the rearrangement of CT examination room in fever clinic, the rearrangement of human resources in radiology department, and the drafting of new operating procedures for radiologists who carry out CT examination on COVID-19 patients. This article also introduced the emergency management procedures of the department of radiology during the outbreak, and the experience of infection prevention for the staff of the department of radiology. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Deficiency of Tfh Cells and Germinal Center in Deceased COVID-19 Patients
In this study, we examined the immune cell compositions in the lung tissues and hilar lymph nodes using immunohistochemistry on 6 deceased COVID-19 patients and 4 focal organizing pneumonia (FOP) patients who underwent lung surgery and served as controls. We found a dominant presence of macrophages and a general deficiency of T cells and B cells in the lung tissues from deceased COVID-19 patients. In contrast to the FOP patients, Tfh cells and germinal center formation were largely absent in the draining hilar lymph nodes in the deceased COVID-19 patients. This was correlated with reduced IgM and IgG levels compared to con...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Prevalence and Influencing Factors on Fatigue of First-line Nurses Combating with COVID-19 in China: A Descriptive Cross-Sectional Study
SummaryNurses ’ work-related fatigue has been recognized as a threat to nurse health and patient safety. The aim of this study was to assess the prevalence of fatigue among first-line nurses combating with COVID-19 in Wuhan, China, and to analyze its influencing factors on fatigue. A multi-center, descriptive, cross-sectional design with a convenience sample was used. The statistical population consisted of the first-line nurses in 7 tertiary general hospitals from March 3, 2020 to March 10, 2020 in Wuhan of China. A total of 2667 samples from 2768 contacted participants completed the investgation, with a response ra...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Screening of SARS-CoV-2 in 299 Hospitalized Children with Hemato-oncological Diseases: A Multicenter Survey in Hubei, China
SummaryThe SARS-CoV-2 infection status of hospitalized children was surveyed in the department of pediatric hematology and oncology in three different hospitals of epidemic areas in Hubei, China. A cross-sectional study was performed to investigate the clinical characteristics, lung CT scan, SARS-CoV-2 nucleic acid test and serum antibodies of hospitalized children with hemato-oncological diseases from January 23 to April 24, 2020. 299 children were enrolled in this study, including 176 males (58.9%) and 123 females (41.1%), aged from 2 months to 16 years. 255 cases (85.3%) received chemotherapy or other immunosuppressive ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Expert Consensus on Nurses ’ Human Caring for COVID-19 Patients in Different Sites
This study aims to develop the expert consensus on nurse ’s human caring for Corona Virus Disease 2019 (COVID-19) patients in different sites, and thus provide a guideline on providing whole process and systematic caring for COVID-19 patients. Based on the frontline experiences of human caring for COVID-19 patients and the review of literature, the init ial draft of consensus was made and finalized after online meeting and revisions. The experts reached consensus on the following parts: terms and definitions, principles of human caring for COVID-19 patients, and human caring measures for COVID-19 patients in differen...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Effects of Different Temperature and Time Durations of Virus Inactivation on Results of Real-time Fluorescence PCR Testing of COVID-19 Viruses
This study aims to investigate the effects of different temperature and time durations of virus inactivation on the results of PCR testing for SARS-CoV-2. Twelve patients at the Renmin Hospital of Wuhan University suspected of being infected with SARS-CoV-2 were selected on February 13, 2020 and throat swabs were taken. The swabs were stored at room temperature (20 –25°C), then divided into aliquots and subjected to different temperature for different periods in order to inactivate the viruses (56°C for 30, 45, 60 min; 65, 70, 80°C for 10, 15, 20 min). Control aliquots were stored at room temperature for ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 7, 2020 Category: Research Source Type: research

Oncological and Reproductive Outcomes of Fertility-sparing Surgery in Women with Early-stage Epithelial Ovarian Carcinoma: A Multicenter Retrospective Study
This study also provides some insight into the attitudes and social factors regarding fertility in EOC patients. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Effect of Periodontal Pathogens on Total Bone Volume Fraction: A Phenotypic Study
This study aimed to further explore the effect of periodontal pathogens on bone volume fraction like bone tissue and mass, and explain the relationship between PD and osteoporosis. Sprague Dawley rats (female, 16 weeks old) were divided into the wild-type (WT) control group (n=9) and PD group (n=9). After eight weeks, periodontal tissues and ligatures, the fourth lumbar vertebra, the femur, the tibia, and blood were extracted and analyzed by micro-computed tomography (micro-CT), hematoxylin and eosin (H&E) staining, tartrate-resistant acid phosphatase (TRAP) staining, polymerase chain reaction (PCR), and enzyme-linked ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Targeted Antagonism of Vascular Endothelial Growth Factor Reduces Mortality of Mice with Acute Respiratory Distress Syndrome
This study attempts to study the targeted drug delivery for ARDS. In this study, we used soluble fms-like tyrosine kinase-1 (sFlt)-targeted ultrasound microbubbles to antagonize the effect of VEGF on lung tissue. Ninety male BALB/c mice were randomly assigned to 6 groups: phosphate buffer saline (PBS) group (PBS+PBS); blank group (PBS+empty microbubbles); lipopolysaccharide (LPS) group (LPS+PBS); ARDS group (LPS+empty microbubbles); control group (PBS+sFlt microbubbles); and treatment group (LPS+sFlt microbubbles). After administration of LPS or PBS in the corresponding groups, the sFlt-targeted microbubbles or empty micro...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice
In conclusion, high-fat diet can lead to IL17A upregulation, VICs myofibroblastic transition and inflammatory cells infiltration in the aortic value of ApoE−/− mice. Blocking IL17A with IL17A mAb can alleviate aortic valve inflammatory states. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Nucleolin Mediates LPS-induced Expression of Inflammatory Mediators and Activation of Signaling Pathways
In this study, we investigated the effects of nucleolin on lipopolysaccharide (LPS)-induced activation of MAPK and NF-KappaB (NF- κB) signaling pathways and secretion of TNF-α, IL-1β and HMGB1 in THP-1 monocytes. Immunofluorescence assay and Western blotting were used to identify the nucleolin expression in cell membrane, cytoplasm and nucleus of THP-1 monocytes. Inactivation of nucleolin was induced by neutralizing antibod y against nucleolin. THP-1 monocytes were pretreated with anti-nucleolin antibody for 1 h prior to LPS challenge. The irrelevant IgG group was used as control. Secretion of inflammatory...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

18 β-Glycyrrhetinic Acid Improves Cardiac Diastolic Function by Attenuating Intracellular Calcium Overload
This study aimed to determine whether 18β-GA can improve the diastolic function and to explore the underlying mechanisms. Eighty male Sprague Dawle y (SD) rats of Langendorff model were randomly divided into the following groups: group A, normal cardiac perfusion group; group B, ischemia-reperfusion group; group C, ischemia-reperfusion with anemoniasulcata toxin II (ATX-II); group D, ranolazine group; and group E, 18β-GA group with four differ ent concentrations. Furthermore, a pressure-overloaded rat model induced by trans-aortic constriction (TAC) was established. Echocardiography and hemodynamics were used to ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Co-expression Network Analysis Identifies Fourteen Hub Genes Associated with Prognosis in Clear Cell Renal Cell Carcinoma
SummaryRenal cancer is a common genitourinary malignance, of which clear cell renal cell carcinoma (ccRCC) has high aggressiveness and leads to most cancer-related deaths. Identification of sensitive and reliable biomarkers for predicting tumorigenesis and progression has great significance in guiding the diagnosis and treatment of ccRCC. Here, we identified 2397 common differentially expressed genes (DEGs) using paired normal and tumor ccRCC tissues from GSE53757 and The Cancer Genome Atlas (TCGA). Then, we performed weighted gene co-expression network analysis and protein-protein interaction network analysis, 17 candidat...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Down-regulation and Clinical Implication of Galectin-9 Levels in Patients with Acute Coronary Syndrome and Chronic Kidney Disease
This study aims to measure the Gal-9 levels in serum and peripheral blood mononuclear cells (PBMCs) in patients with ACS plus CKD and examine their clinical implication. The serum levels of Gal-9 were determined by enzyme-linked immunosorbent assay (ELISA), the expression levels of Gal-9, Tim-3, and Foxp3 mRNA in PBMCs were detected by real-time reverse transcription-polymerase chain reaction (RT-PCR), and the expression of Gal-9 on the surface of PBMCs and in PBMCs was analyzed by flow cytometry. Furthermore, the correlation of serum Gal-9 levels with anthropometric and biochemical variables in patients with ACS plus CKD ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Neuroprotective Effects of Dexmedetomidine Preconditioning on Oxygen-glucose Deprivation-reoxygenation Injury in PC12 Cells via Regulation of Ca 2+ -STIM1/Orai1 Signaling
In conclusion, these results suggested that DEX exerts neuroprotective effects against oxidative stress, autophagy, and neuronal apoptosis after OGD/R injury via modulation of Ca2+-STIM1/Orai1 signaling. Our results offer insights into the molecular mechanisms of DEX in protecting against neuronal ischemia-reperfusion injury. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Cancer-testis Antigen OY-TES-1 Expression and Immunogenicity in Hepatocellular Carcinoma
This study was to evaluate OY-TES-1 expression and immunogenicity in hepatocelluar carcinoma (HCC). OY-TES-1 mRNA expression was detected in 56 HCC tissues and 5 normal liver tissues by reverse transcriptase PCR (RT-PCR). Of the 56 cases of HCC tissues tested, 37 cases had tumor and matched adjacent non-cancer tissues and were subjected to both RT-PCR and quantitative real-time PCR. OY-TES-1 protein was subsequently observed on a panel of tissue microarrays. Sera from patients were tested for OY-TES-1 antibody by ELISA. To identify OY-TES-1 capable of inducing cellular immune response, OY-TES-1 protein was used to sensitiz...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Treatment of Infertile Women with Unilateral Tubal Occlusion Diagnosed by Hysterosalpingography: The Role of Intrauterine Insemination
In conclusion, the clinical outcomes of IUI were worse in patients with unilateral tubal occlusion than in those with unexplained infertility. This might be primarily caused by the worse outcome of patients with unilateral mid-distal tubal occlusion instead of proximal occlusion. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Use of Muscle Feeding Arteries as Recipient Vessels for Soft Tissue Reconstruction in Lower Extremities
SummaryMicrosurgical free tissue transfer is still playing an important role in lower extremity reconstruction. Finding a suitable recipient artery for anastomosis is critical in the microsurgical procedure, especially in an extensive wound, or in a complex trauma combined with vascular injury. From April 2014 to March 2018, we retrospectively reviewed patients with traumatic/post-traumatic, oncologic, and electrical wounds in the lower extremity. Those treated with muscle feeding artery as recipient vessels were included. The latissimus dorsi (LD) muscle free flap, anterior lateral thigh (ALT) perforator free flap, and de...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Nutrition Support Practices of Hematopoietic Stem Cell Transplantation Centers in Mainland China
SummaryIt has been identified that malnutrition can influence the immune system and time of engraftment, and it ’s also associated with increased incidence of complications, prolonged length of hospital stays, and transplant mortality and morbidity in patients undergoing hematopoietic stem cell transplantation (HSCT), so dynamic nutrition care is highly important. The aim of this study was to better underst and the differences between clinical nutrition practices and international recommendations as well as possible barriers to the use of nutrition support in HSCT patients. An evidence-based nutrition support pathway...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Oleic Acid (OA), A Potential Dual Contrast Agent for Postmortem MR Angiography (PMMRA): A Pilot Study
The objective of this research is to compare MR images of oleic acid (OA) with paraffin oil (PO)in vitro and inex situ animal hearts, in order to evaluate the feasibility to use OA as a novel contrast agent for PMMRA.In vitro, OA, PO and water (control) were introduced into three tubes separately and T1weighted-spin echo (T1w-SE) and T2w-SE images were acquired on a 1.5T MR scanner. In the second experiment, OA and PO were injected into left coronary artery (LCA) and left ventricle (LV) ofex situ bovine hearts and their T1w-SE, T2w-SE, T1w-multipoint Dixon (T1w-mDixon) and 3DT2w-mDixon images were acquired. The overall res...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - August 1, 2020 Category: Research Source Type: research

Changes of Serum Insulin-like Growth Factor-2 Response to Negative Symptom Improvements in Schizophrenia Patients Treated with Atypical Antipsychotics
SummaryAccumulating evidence suggests that a disruption of early brain development, in which insulin-like growth factor-2 (IGF-2) has a crucial role, may underlie the pathophysiology of schizophrenia. Our previous study has shown that decreased serum IGF-2 was correlated with the severity of psychopathology in patients with schizophrenia. Here we conducted a prospective observation trial to investigate the effects of atypical antipsychotics on serum IGF-2 level and its relationship with clinical improvements in schizophrenia patients. Thirty-one schizophrenia patients with acute exacerbation and 30 healthy individuals were...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

N 6 -methyladenosine as a Novel Regulator of Brain Physiology and Diseases
SummaryN6-methyladenosine (m6A) is identified as the most widespread and abundant internal chemical modification of RNA in eukaryotes. A series of proteins including methyltransferases (also known as “writers”), demethylases (also known as “erasers”), and m6A-binding proteins (also known as “readers”) were indicated to participate in the m6A methylation. m6A has emerged as a regulator of various cellular, developmental, and disease processes. Notably, there is highest abundance of m6A methylation in brain than in other organs, which indicates that m6A plays an essential role in brain fun...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Households Hunger: The Key Attribute to Anthropometric Failures of Children in West Oromia (Ethiopia)
SummaryPoor nutrition is the underlying cause of child death. However, comprehensive data showing the relationships between dietary-practices, food security, and nutritional status are scant. The present study aimed to examine the association of inappropriate feeding practices and household-hunger with anthropometric measures in children aged 6 –23 months. A cross-sectional survey was conducted on randomly selected 525-households. Semi-structured interviewer-administered questionnaires were used to gather data on socio-demographic, child health, dietary-practices and household-hunger. Weight and height/length of the ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

S-methyl-L-cysteine Protects against Antimycin A-induced Mitochondrial Dysfunction in Neural Cells via Mimicking Endogenous Methionine-centered Redox Cycle
In this study, we observed that the natural L-Met acted as a good scavenger for antimycin A-induced mitochondrial superoxide overproduction in PC12 cells. This antioxidation was largely dependent on the Met oxidase activity of MsrA. S-methyl-L-cysteine (SMLC), a natural analogue of Met that is abundantly found in garlic and cabbage, could activate the Met oxidase activity of MsrA to scavenge free radicals. Furthermore, SMLC protected against antimycin A-induced mitochondrial membrane depolarization and alleviated 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity. Thus, our data highlighted the possibility for SMLC s...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Yu Gan Long Ameliorates Hepatic Fibrosis by Inhibiting PI3K/AKT, Ras/ERK and JAK1/STAT3 Signaling Pathways in CCl 4 -induced Liver Fibrosis Rats
In this study, we further investigated the mechanism of YGL reducing carbon tetrachloride (CCl4)-induced liver fibrosis in rats. Our results showed that YGL suppressed CCl4-induced upregulation of collagen IV (Col IV), type HI precollagen (PCHI), hyaluronuc acid (HA) and laminin (LN), which are implicated in liver fibrosis. Also, YGL reduced the α-smooth muscle actin (α-SMA) expression, which acts as the indicator of liver fibrosis. Furthermore, YGL decreased the serum levels of hepatic stellate cell (HSC) mitogen PDGF-BB and inflammation cytokines, including TNF-α, IL-1β, IL-6. Markers involved in l...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Increased Preoperative Glucose Variability Is Associated with Adverse Perioperative Outcomes Following Orthopedic Surgery in Patients with Type 2 Diabetes Mellitus
SummaryThe association between glucose variability (GV) and adverse perioperative outcomes in type 2 diabetes mellitus (T2DM) patients undergoing orthopedic surgery was investigated. A retrospective cohort study was performed by analyzing data on T2DM patients receiving continuous blood glucose (BG) monitoring and continuous subcutaneous insulin infusion treatment due to poorly controlled preoperative BG prior to orthopedic surgery. GV was assessed with coefficient of variation (CV). Postoperative and perioperative CV, hypoglycemia cases, and other perioperative outcomes (diabetes preparation time [DPT], length of stay [LO...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Light Cupula: To Be Or Not to Be?
In this study, we looked at the clinical features of light cuplula and discussed the possible mechanisms and therapeutic strategies of the condition. The notion o f light cupula is a helpful addition to the theory of peripheral positional vertigo and nystagmus. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

The Role of Ankaferd Blood Stopper and Oxytocin as Potential Therapeutic Agents in Endometriosis: A Rat Model
SummaryTo evaluate the potential effect of Ankaferd Blood Stopper (ABS) and oxytocin (OT) in an experimental endometriosis model, 18 female Sprague Dawley rats were used in this study. The animals were divided randomly into three groups after surgical induction of endometriosis: group 1: control group (isotonic NaCl, 1 mL/kg/day, intramuscular,n=6); group 2: OT group (OT, 80 U/kg/day, intramuscular,n=6); group 3: ABS group (ABS, 1.5 mL/kg/day, intraperitoneal,n=6). Each group was treated for four weeks (two times per week). Volumes of endometriotic explants were measured in biopsy samples for histopathological analysis. Va...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Microglia: A Central Player in Depression
SummaryMicroglia are the major immune cells in the central nervous system and play a key role in the normal function of the brain. Microglia exhibit functional diversity, and they control the inflammation in central nervous system through releasing inflammatory cytokine, clearing apoptotic cells via phagocytosis, regulating synaptic plasticity and the formation of neural network by synapse pruning. Recent studies have strongly indicated that the microglial dysfunction is associated with a variety of neuropsychiatric diseases such as depression, which have been termed as “microgliopathy”. The emergency of advanc...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Mechanism of Cross-modal Information Influencing Taste
SummaryStudies on the integration of cross-modal information with taste perception has been mostly limited to uni-modal level. The cross-modal sensory interaction and the neural network of information processing and its control were not fully explored and the mechanisms remain poorly understood. This mini review investigated the impact of uni-modal and multi-modal information on the taste perception, from the perspective of cognitive status, such as emotion, expectation and attention, and discussed the hypothesis that the cognitive status is the key step for visual sense to exert influence on taste. This work may help rese...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Expression of Dickkopf-related Protein 1 in Patients with Temporomandibular Osteoarthritis after Treatment with Hyaluronic Acid
In this study, we assessed the expression of DKK1 in the synovial fluid of TMD-OA patients before and after treatment with HA via enzyme-linked immunosorbent assay. We also investigated the role of DKK1 in TMD-OA via immunohistochemical staining. The relationship between the expression of DKK1 and the clinicopathological characteristics was determined by Pearson analysis. The results showed that the expression of DKK1 was significantly decreased after treatment with HA. Correlation analyses indicated that the expression of DKK1 in the TMD-OA samples was closely correlated with mouth opening and pain. These findings suggest...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

The Recent Advances of Fluorescent Sensors Based on Molecularly Imprinted Fluorescent Nanoparticles for Pharmaceutical Analysis
SummaryFluorescent nanoparticles have good chemical stability and photostability, controllable optical properties and larger stokes shift. In light of their designability and functionability, the fluorescent nanoparticles are widely used as the fluorescent probes for diverse applications. To enhance the sensitivity and selectivity, the combination of the fluorescent nanoparticles with the molecularly imprinted polymer, i.e. molecularly imprinted fluorescent nanoparticles (MIFN), was an effective way. The sensor based on MIFN (the MIFN sensor) could be more compatible with the complex sample matrix, which was especially wid...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Berberine Inhibits Gluconeogenesis in Skeletal Muscles and Adipose Tissues in Streptozotocin-induced Diabetic Rats via LKB1-AMPK-TORC2 Signaling Pathway
SummaryThe effect and potential molecular mechanisms of berberine on gluconeogenesis in skeletal muscles and adipose tissues were investigated. After adaptive feeding for one week, 8 rats were randomly selected as the normal group and fed on a standard diet. The remaining 32 rats were fed on a high-fat diet and given an intravenous injection of streptozotocin (STZ) for 2 weeks to induce the diabetic models. The diabetic rat models were confirmed by oral glucose tolerance test (OGTT) and randomly divided into 4 groups (n=8 each), which were all fed on a high-fat diet. Berberine (3 g/kg per day) or metformin (183 mg/kg per d...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

TfR1 Extensively Regulates the Expression of Genes Associated with Ion Transport and Immunity
SummaryTransferrin receptor 1 (TfR1), encoded by theTFRC gene, is the gatekeeper of cellular iron uptake for cells. A variety of molecular mechanisms are at work to tightly regulate TfR1 expression, and abnormal TfR1 expression has been associated with various diseases. In the current study, to determine the regulation pattern of TfR1, we cloned and overexpressed the humanTFRC gene in HeLa cells. RNA-sequencing (RNA-seq) was used to analyze the global transcript levels in overexpressed (OE) and normal control (NC) samples. A total of 1669 differentially expressed genes (DEGs) were identified between OE and NC. Gene ontolog...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Potential of Arbidol for Post-exposure Prophylaxis of COVID-19 Transmission: A Preliminary Report of a Retrospective Cohort Study
SummaryThe efficient transmission of severe acute respiratory syndrome-2 coronavirus (SARS-CoV-2) from patients to health care workers or family members has been a worrisome and prominent feature of the ongoing outbreak. On the basis of clinical practice andin-vitro studies, we postulated that post-exposure prophylaxis (PEP) using Arbidol is associated with decreased infection among individuals exposed to confirmed cases of COVID-19 infection. We conducted a retrospective cohort study on family members and health care workers who were exposed to patients confirmed to have SARS-CoV-2 infection by real-time RT-PCR and chest ...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Cerebellar Degeneration-related Antigen 1 Is Ubiquitously Expressed in Human Epidermis and Dermis
SummaryCerebellar degeneration-related antigen 1 (CDR1) was described to be expressed in the nervous system and in different types of cancer tissues. In the present study, we demonstrate that CDR1 is in addition ubiquitously expressed in human epidermis, dermis and isolated skin cells. Both CDR1 mRNA and protein were detected in human skin-derived mast cells, melanocytes, fibroblasts and keratinocytes, suggesting that CDR1 does not have a neuron-specific function. (Source: Journal of Huazhong University of Science and Technology -- Medical Sciences --)
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Erratum to: Kinetic Characterization of Tyrosinase-catalyzed Oxidation of Four Polyphenols
The article “Kinetic Characterization of Tyrosinase-catalyzed Oxidation of Four Polyphenols”, written by Wan-yu LIU, Congming ZOU, Jian-hua HU, Zi-jun XU, Lu-qin SI, Jun-jun LIU, Jian-geng HUANG, was originally published electronically on the publisher’s internet portal on May 2020 without open access. Wi th the author(s)’ decision to opt for Open Choice, the copyright of the article is changed to © The Author(s) 2020 and the article is forthwith distributed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits use, sharin...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Erratum to: Polysubstituted Phenyl Glucosides Produced by the Fungus Metarrhizium anisopliae
The article “Polysubstituted Phenyl Glucosides Produced by the FungusMetarrhizium anisopliae”, written by Wen-jing WANG, Chong DAI, Jian-ping WANG, Hu-cheng ZHU, Chun-mei CHEN, Yong-hui ZHANG, was originally published electronically on the publisher’s internet portal on May 2020 without open access. With the author(s)’ decision to opt for Open Choice, the copyright of the article is changed to © The Author(s) 2020 and the article is forthwith distributed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, ada...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Erratum to: The Role of CARD9 in Metabolic Diseases
The article “The Role of CARD9 in Metabolic Diseases”, written by Cheng TIAN, Ya-li TUO, Yi LU, Chuan-rui XU, Ming XIANG, was originally published electronically on the publisher’s internet portal on May 2020 without open access. With the author(s)’ decision to opt for Open Choice, the copyright of the article is changed to © The Author(s) 2020 and the article is forthwith distributed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - June 1, 2020 Category: Research Source Type: research

Adjustment Disorder in Female Breast Cancer Patients: Prevalence and Its Accessory Symptoms
SummaryWomen diagnosed with breast cancer may have serious psychological problems and will suffer from adjustment disorder (AjD). We investigated the prevalence of AjD in female breast cancer patients who were diagnosed within 1 year and examined the severe life events they experienced, and the most common symptoms of AjD. 342 newly diagnosed (
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - May 30, 2020 Category: Research Source Type: research

Relationship between Methylation of FHIT and CDH13 Gene Promoter Region and Liver Cancer
SummaryIn order to demonstrate the relationship between methylation of fragile histidine triad (FHIT) and T-cadherin/H-cadherin (CDH13) genes and liver cancer, the methylation status of FHIT and CDH13 was detected in healthy individuals and in Mongolian and Han patients with liver cancer. The phenol-chloroform method was used to extract genomic DNA. The methylation specific polymerase chain reaction method was applied to detect the methylation status of FHIT and CDH13. The relationship between smoking and alcohol consumption and gene (FHIT and CDH13) methylation was analyzed. There was significant difference in methylation...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - May 30, 2020 Category: Research Source Type: research

Progressively Enlarging Goiter: Case Reports of Primary Thyroid Lymphoma and Literature Review
We report three typical cases of PTL referred to our hospital. All three cases had long history of Hashimoto ’s thyroiditis, and presented with progressively enlarging neck mass. The first two cases were confirmed by surgical biopsy to be diffuse large B cell lymphoma, and received radiotherapy combined with chemotherapy, or received only chemotherapy. The third case was confirmed by core needle biopsy t o be mucosa-associated lymphoid tissue lymphoma, and received radiotherapy. In summary, confirmation of PTL diagnosis is essential for further clinical decisions. Core biopsy should be one of the most important metho...
Source: Journal of Huazhong University of Science and Technology -- Medical Sciences -- - May 30, 2020 Category: Research Source Type: research