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Specialty: Allergy & Immunology

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Total 1296 results found since Jan 2013.

siRNA containing a unique 5-nucleotide motif acts as a quencher of IFI16-mediated innate immune response
Publication date: October 2019Source: Molecular Immunology, Volume 114Author(s): Hongyan Sui, Jun Yang, Xiaojun Hu, Qian Chen, Tomozumi ImamichiAbstractWe previously reported that some small interfering RNA (siRNA) enhances DNA or DNA virus mediated-interferon (IFN)-λ1(a type III IFN) induction through the crosstalk between retinoic acid-inducible gene I (RIG-I) and interferon gamma-inducible protein 16 (IFI16) signalling pathway. Here we provide further evidence of a new role for siRNA. siRNA containing a 5-nucleotide (nt) motif sequence suppresses DNA-mediated not only type III IFNs, but also type I IFNs and inflammator...
Source: Molecular Immunology - August 21, 2019 Category: Allergy & Immunology Source Type: research

A review of PD-1/PD-L1 siRNA delivery systems in immune T cells and cancer cells
CONCLUSION: The findings of this study indicated that several strategies for siRNA transfection to immune and cancer cells have been evaluated in recent decades, some of which effectively transfect siRNA to target cells, and defined PD-1 siRNA as a promising strategy for cancer treatment.PMID:35987146 | DOI:10.1016/j.intimp.2022.109022
Source: International Immunopharmacology - August 20, 2022 Category: Allergy & Immunology Authors: Mehdi Barati Farshad Mirzavi Mahdi Atabaki Bahram Bibak Mojgan Mohammadi Mahmoud Reza Jaafari Source Type: research

The siRNA cocktail targeting IL-10 receptor and TGF-β receptor on dendritic cells potentiates tumor antigen-specific CD8(+) T cell immunity.
This article is protected by copyright. All rights reserved. PMID: 25753156 [PubMed - as supplied by publisher]
Source: Clinical and Developmental Immunology - March 8, 2015 Category: Allergy & Immunology Authors: Ahn YH, Hong SO, Kim JH, Noh KH, Song KH, Lee YH, Jeon JH, Kim DW, Seo JH, Kim TW Tags: Clin Exp Immunol Source Type: research

Chitosan nanoparticles as a dual drug/siRNA delivery system for treatment of colorectal cancer.
In conclusion, ChNPs encapsulating DOX and snail siRNA can be considered as an effective anti-cancer drugs delivery system for the treatment of colorectal cancer. PMID: 27916629 [PubMed - as supplied by publisher]
Source: Immunology Letters - November 30, 2016 Category: Allergy & Immunology Authors: Sadreddini S, Safaralizadeh R, Baradaran B, Aghebati-Maleki L, Hosseinpour-Feizi MA, Shanehbandi D, Jadidi-Niaragh F, Sadreddini S, Kafil HS, Younesi V, Yousefi M Tags: Immunol Lett Source Type: research

Activation of Cell Membrane-localized Toll-like Receptor 3 by siRNA.
Abstract Small interfering RNA molecules (siRNA) are short dsRNAs that are used for different therapeutic applications. On the other hand, dsRNAs can bind to and activate cell RNA sensors and consequently trigger inflammatory response. Here we show that siRNA activates primary human endothelial cells and human lymphatic endothelial cells and that this response is inhibited by antibodies against TLR3. In contrast, the activation of human lymphatic endothelial cells by poly(I:C) was inhibited by bafilomycin but not by anti-TLR3 antibodies. Bafilomycin also inhibited poly(I:C) but not siRNA cell stimulation in TLR3-t...
Source: Immunology Letters - April 6, 2017 Category: Allergy & Immunology Authors: Pirher N, Pohar J, Manček-Keber M, Benčina M, Jerala R Tags: Immunol Lett Source Type: research

The siRNA cocktail targeting IL‐10 receptor and TGF‐β receptor on dendritic cells potentiates tumor antigen‐specific CD8+ T cell immunity
This article is protected by copyright. All rights reserved.
Source: Clinical and Experimental Immunology - March 8, 2015 Category: Allergy & Immunology Authors: Ye‐Hyeon Ahn, Soon‐Oh Hong, Jin Hee Kim, Kyung Hee Noh, Kwon‐Ho Song, Young‐Ho Lee, Ju‐Hong Jeon, Dong‐Wan Kim, Jae Hong Seo, Tae Woo Kim Tags: Original Article Source Type: research

The siRNA cocktail targeting interleukin 10 receptor and transforming growth factor‐β receptor on dendritic cells potentiates tumour antigen‐specific CD8+ T cell immunity
In this study, we used small interfering RNA (siRNA) to silence the expression of endogenous molecules in DCs, which can sense immunosuppressive factors. Among the siRNAs targeting various immunosuppressive molecules, we observed that DCs transfected with siRNA targeting IL‐10 receptor alpha (siIL‐10RA) initiated the strongest antigen‐specific CD8+ T cell immune responses. The potency of siIL‐10RA was enhanced further by combining it with siRNA targeting TGF‐β receptor (siTGF‐βR), which was the next best option during the screening of this study, or the previously selected immunoadjuvant siRNA targeting phosp...
Source: Clinical and Experimental Immunology - May 17, 2015 Category: Allergy & Immunology Authors: Y.‐H. Ahn, S.‐O. Hong, J. H. Kim, K. H. Noh, K.‐H. Song, Y.‐H. Lee, J.‐H. Jeon, D.‐W. Kim, J. H. Seo, T. W. Kim Tags: Original Article Source Type: research

Complement C2 siRNA mediated therapy of myasthenia gravis in mice.
In this study, we show that inhibition of complement cascade activation by suppressing the expression of a critical low-abundant protein, C2, in the classical complement pathway, significantly improved clinical and immunopathological manifestations of EAMG. Two weeks after a second booster immunization with acetylcholine receptor, when mice exhibit muscle weakness, i.p. injection of C2 siRNA significantly suppressed C2 mRNA in the blood cells and liver of EAMG mice. Treatment of EAMG mice with C2 siRNA, once a week for 5 weeks, significantly improved muscle strength, which was further evidenced by functional AChR preservat...
Source: Journal of Autoimmunity - February 11, 2013 Category: Allergy & Immunology Authors: Huda R, Tüzün E, Christadoss P Tags: J Autoimmun Source Type: research

A procedure for efficient non-viral siRNA transfection of primary human monocytes using nucleofection.
Abstract Monocytes are an important constituent of the innate immune system. Therefore, manipulating gene expression of primary human monocytes is a crucial mean to study and characterize the functions of targeted proteins in monocytes. Gene silencing by transfection of cells with small interfering RNA (siRNA) leading to degradation of the corresponding mRNA and thus to reduced target protein levels is an important tool to investigate gene and protein function of interest. However, non-viral transfection of primary monocytes is challenging because siRNA uptake by these suspended cells is tricky, the individual cel...
Source: Journal of Immunological Methods - April 16, 2015 Category: Allergy & Immunology Authors: Scherer O, Maeß MB, Lindner S, Garscha U, Weinigel C, Rummler S, Werz O, Lorkowski S Tags: J Immunol Methods Source Type: research

Blimp-1 siRNA inhibits B cell differentiation and prevents the development of lupus in mice.
Abstract Cumulative evidence suggest that B-lymphocytes play a role in the development of systemic lupus erythematosus (SLE). Thus, the therapeutic approach targeting specific B cells provides a promising way to treat SLE. Blimp-1 (B lymphocyte induced maturation protein), a transcriptional factor, controls the terminal differentiation of mature B cells to plasma cells. To explore the potential of Blimp-1 in the SLE development, we constructed the adenovirus encoding Blimp-1 siRNA, and injected it into BWF1 lupus mice. The results demonstrated that Blimp-1 siRNA decreased the Blimp-1 expression of B cells by regul...
Source: Human Immunology - December 7, 2012 Category: Allergy & Immunology Authors: Zhou Z, Ren Y, Hu Z Tags: Hum Immunol Source Type: research

Exosomes from iPSCs Delivering siRNA Attenuate Intracellular Adhesion Molecule-1 Expression and Neutrophils Adhesion in Pulmonary Microvascular Endothelial Cells
In this study, we reprogrammed urine exfoliated renal epithelial cells into human induced pluripotent stem cells (huiPSCs) and purified the exosomes (Exo) from huiPSCs as RNAi delivery system. Through choosing the episomal system to deliver transcription factors, we obtained a non-integrating huiPSCs. Experiments in bothvitro andvivo demonstrated that these huiPSCs possess the pluripotent properties. The exosomes of huiPSCs isolated by differential centrifugation were visualized by transmission electron microscopy (TEM) showing a typical exosomal appearance with an average diameter of 122  nm. Immunoblotting confirmed the...
Source: Inflammation - December 20, 2016 Category: Allergy & Immunology Source Type: research

Culture medium used during small interfering RNA (siRNA) transfection determines the maturation status of dendritic cells.
Abstract Gene silencing using small interfering ribonucleic acids (siRNA) is a powerful method to interfere with gene expression, allowing for the functional exploration of specific genes. siRNA interference can be applied in both cell lines, as well as in primary, non-dividing cell types like dendritic cells. However, the efficacy in different cell types is variable and requires optimization. Here, we showed that the type of culture medium used during lipid-based siRNA-mediated transfection acts as a critical factor, affecting dendritic cell activation. Transfection of immature monocyte-derived dendritic cells in...
Source: Journal of Immunological Methods - January 16, 2020 Category: Allergy & Immunology Authors: van Essen MF, Schlagwein N, van Gijlswijk-Janssen DJ, Anholts JDH, Eikmans M, Ruben JM, van Kooten C, COMBAT consortium Tags: J Immunol Methods Source Type: research

Nanoparticle-facilitated delivery of BAFF-R siRNA for B cell intervention and rheumatoid arthritis therapy.
Abstract The present study was designed to explore the effects of B-cell activating factor receptor (BAFF-R) siRNA encapsulated nanoparticles on collagen-induced arthritis (CIA). BAFF-R siRNA encapsulated nanoparticles (NP-siBAFF-R) were constructed using a double emulsion method and was characterized by dynamic light scattering and transmission electron microscopy. Cellular uptake of nanoparticles was determined using flow cytometry. The CIA mouse model was established and the mice were intravenously injected with nanoparticles. NP-siBAFF-R effectively decreased the expression of BAFF-R in B cells and facilitated...
Source: International Immunopharmacology - August 27, 2020 Category: Allergy & Immunology Authors: Wu H, Su S, Wu Y, Wu Y, Zhang Z, Chen Q Tags: Int Immunopharmacol Source Type: research

The Two Hit Hypothesis: An Improved Method for siRNA-Mediated Gene Silencing in Stimulated Primary Human T Cells.
In this study we found that the recommended programs for nucleofection of stimulated primary human T cells with siRNAs inhibited cellular proliferation and were associated with a significant loss of cell viability. Furthermore, viable cells that survived the nucleofection procedure were perturbed in their ability to polarise in response to chemokine stimulation in comparison to mock nucleofections. We therefore evaluated other nucleofection programs and highlight one that resulted in significant silencing at the protein level following nucleofection with siRNAs, while maintaining cell viability and responsiveness to chemok...
Source: Journal of Immunological Methods - August 26, 2013 Category: Allergy & Immunology Authors: Freeley M, Long A Tags: J Immunol Methods Source Type: research