Complement and its environmental determinants in the progression of human rheumatoid arthritis
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Elizabeth A. Bemis, Jill M. Norris, Jennifer Seifert, Ashley Frazer-Abel, Yuko Okamoto, Marie L. Feser, M. Kristen Demoruelle, Kevin D. Deane, Nirmal K. Banda, V. Michael HolersAbstractRheumatoid arthritis (RA) is a complex autoimmune disease with an etiology that is not yet well understood, disproportionally affects women and also varies in incidence and prevalence by population. The presence of anti-citrullinated protein antibodies (ACPA) is a highly specific biomarker for the diagnosis of clinically apparent RA. ACPA are also present in the ...
Source: Molecular Immunology - June 15, 2019 Category: Allergy & Immunology Source Type: research

TLR1/TLR2 signaling blocks the suppression of monocytic myeloid-derived suppressor cell by promoting its differentiation into M1-type macrophage
In this study, we found that TLR1/TLR2 expression predicted favorable prognosis of lung cancer patients. In the related mice tumor model, TLR1/TLR2 activation by synthetic bacterial lipoprotein (BLP), a TLR1/2 agonist, greatly inhibited tumor growth and selectively decreased monocytic MDSCs (M-MDSCs). Furthermore, BLP treatment redirected M-MDSC differentiation towards M1 macrophage through JNK pathway, and thus blocked the suppressive activity of M-MDSCs in a TLR2-dependent manner. Therefore, our data demonstrated that TLR2 could be a promising biomarker and a potential immunotherapeutic target for lung cancer. (Source: Molecular Immunology)
Source: Molecular Immunology - June 15, 2019 Category: Allergy & Immunology Source Type: research

Nimbolide ameliorates unilateral ureteral obstruction-induced renal fibrosis by inhibition of TGF-β and EMT/Slug signalling
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Shivaraju Annaldas, Mohd Aslam Saifi, Amit Khurana, Chandraiah GoduguAbstractChronic kidney disease (CKD) involves interstitial fibrosis as an underlying pathological process associated with compromised renal function irrespective of etiological cause of the injury. The transforming growth factor-β (TGF-β) plays a pivotal role in progression of renal fibrosis. TGF-β transduces its downstream signalling by phosphorylation of smad2/3 and also regulates epithelial-mesenchymal-transition (EMT), a program centrally involved in activat...
Source: Molecular Immunology - June 13, 2019 Category: Allergy & Immunology Source Type: research

The role of complement in antibody mediated transplant rejection
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Joshua M. Thurman, Sarah E. Panzer, Moglie Le QuintrecAbstractAntibody mediated transplant rejection (AMR) is a major cause of long-term allograft failure, and currently available treatments are of limited efficacy for treating the disease. AMR is caused by donor specific antibodies (DSA) that bind to antigens within the transplanted organ. DSA usually activate the classical pathway of complement within the allograft, and complement activation is believed to be an important cause of tissue injury in AMR. Several new clinical assays may improve ...
Source: Molecular Immunology - June 11, 2019 Category: Allergy & Immunology Source Type: research

Molecular genetic characterization and haplotype diversity of swine leukocyte antigen in Chinese Rongshui miniature pigs
In this study, we identified 42 alleles at eight polymorphic SLA loci (SLA-1, SLA-3, SLA-2, SLA-6, DRA, DRB1, DQA, and DQB1) representing seven class I and six class II haplotypes using reverse transcription-polymerase chain reaction (RT-PCR) sequence-based typing and PCR-sequence specific primers in Rongshui miniature pigs. The official names were designated by the SLA Nomenclature Committee of the International Society for Animal Genetics. Seven class I haplotypes, Hp-5b.0, 86.0, 87.0, 88.0, 89.0, 90.0 and 91.0, and four class II haplotypes, Hp-0.18b, 0.19c, 0.41 and 0.47, had not previously been reported in other pig br...
Source: Molecular Immunology - June 7, 2019 Category: Allergy & Immunology Source Type: research

An overview of lipodystrophy and the role of the complement system
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): F. Corvillo, B. AkinciAbstractThe complement system is a major component of innate immunity playing essential roles in the destruction of pathogens, the clearance of apoptotic cells and immune complexes, the enhancement of phagocytosis, inflammation, and the modulation of adaptive immune responses. During the last decades, numerous studies have shown that the complement system has key functions in the biology of certain tissues. For example, complement contributes to normal brain and embryonic development and to the homeostasis of lipid metabol...
Source: Molecular Immunology - June 7, 2019 Category: Allergy & Immunology Source Type: research

Decreased number of CD19+CD24hiCD38hi regulatory B cells in Diabetic nephropathy
This study aimed to examine the putative contribution of regulatory B cells to the pathogenesis of DN. The number of circulating CD19+CD24hiCD38hi B cells, CD19+CD24hiCD38hiCD5+ B cells, and CD19+CD24hiCD38hiIL-10+ B cells were significantly lower in DN patients (p 
Source: Molecular Immunology - June 7, 2019 Category: Allergy & Immunology Source Type: research

AID preferentially targets the top strand in nucleosome sequences
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Amit Kumar Singh, Ankit Jaiswal, Prashant KodgireAbstractAID initiates both somatic hypermutation (SHM) and class switch recombination (CSR) in Ig genes. AID-induced mutations are linked with transcription initiation and elongation. Transcription occurs in the context of chromatin and thus RNA PolII and AID need to deal with nucleosomes. Both nucleosome stability and positioning significantly influence the accessibility of AID to Ig genes and the SHM pattern. Interestingly, in the nucleosome, SHM process seems to have a preference for the top s...
Source: Molecular Immunology - June 7, 2019 Category: Allergy & Immunology Source Type: research

Inducible overexpression of zebrafish microRNA-722 suppresses chemotaxis of human neutrophil like cells
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Alan Y. Hsu, Sheng Liu, Ramizah Syahirah, Kent A. Brasseale, Jun Wan, Qing DengAbstractNeutrophil migration is essential for battling against infections but also drives chronic inflammation. Since primary neutrophils are terminally differentiated and not genetically tractable, leukemia cells such as HL-60 are differentiated into neutrophil-like cells to study mechanisms underlying neutrophil migration. However, constitutive overexpression or inhibition in this cell line does not allow the characterization of the genes that affect the differenti...
Source: Molecular Immunology - June 7, 2019 Category: Allergy & Immunology Source Type: research

Editorial Board
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): (Source: Molecular Immunology)
Source: Molecular Immunology - June 5, 2019 Category: Allergy & Immunology Source Type: research

Corrigendum to: “CD8+iTregs attenuate glomerular endothelial cell injury in lupus-prone mice through blocking the activation of p38 MAPK and NF-Κb” [Molecular Immunology Volume 103 (2018) Pages 133-143]
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Ya Liu, Weijuan Deng, Qiaoyun Meng, Xiaonan Qiu, Dong Sun, Chun Dai (Source: Molecular Immunology)
Source: Molecular Immunology - June 5, 2019 Category: Allergy & Immunology Source Type: research

Emodin ameliorates rheumatoid arthritis by promoting neutrophil apoptosis and inhibiting neutrophil extracellular trap formation
In this study, we evaluated the effect of emodin on a murine adjuvant-induced arthritis (AA) model of RA in vivo and on neutrophil apoptosis and NETosis in vitro. Our results show that emodin alleviated AA by reducing neutrophil infiltration and proinflammatory cytokine (interleukin-6, interferon-gamma and tumor necrosis factor-α) release. Emodin promoted apoptosis and inhibited autophagy and NETosis in neutrophils. These findings indicate that emodin represents a potential therapeutic agent for RA. (Source: Molecular Immunology)
Source: Molecular Immunology - June 5, 2019 Category: Allergy & Immunology Source Type: research

Dissociation kinetics of TAPBPR-MHC class I complex
Publication date: Available online 4 June 2019Source: Molecular ImmunologyAuthor(s): Steve Cho, Jonghoon Kang (Source: Molecular Immunology)
Source: Molecular Immunology - June 4, 2019 Category: Allergy & Immunology Source Type: research

MTB driven B cells producing IL-35 and secreting high level of IL-10 in the patients with active pulmonary tuberculosis
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): You-Chao Dai, Wan-Dang Wang, Jun-Ai Zhang, Chen Chen, Hou-Long Luo, Huan Xu, Ying Peng, Hong Luo, Xu-Ran Yang, Xinchun Chen, Xian-Jin Wu, Guang-Hui Chen, Zheng W. Chen, Jun-Fa XuAbstractRegulatory B cells (Bregs) have critical roles as a negative regulator of immunity, mainly due to the fact that it secrets high a level of interleukin 10 (IL-10). Recently, a new subset of Bregs was identified as a key source of IL-35, which is an immunosuppressive cytokine and conventionally thought to be secreted by regulatory T cells (Tregs). Our previous stu...
Source: Molecular Immunology - June 4, 2019 Category: Allergy & Immunology Source Type: research

Sequencing of VDJ genes in Lepus americanus confirms a correlation between VHn expression and the leporid species continent of origin
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Ana Pinheiro, Patricia de Sousa-Pereira, Tereza Almeida, Catarina C. Ferreira, Josée-Anne Otis, Melanie R. Boudreau, Jacob L. Seguin, Dennis K. Lanning, Pedro J. EstevesAbstractLeporid VH genes used in the generation of their primary antibody repertoire exhibit highly divergent lineages. For the European rabbit (Oryctolagus cuniculus) four VHa lineages have been described, the a1, a2, a3 and a4. Hares (Lepus spp.) and cottontail (Sylvilagus floridanus) express one VHa lineage each, the a2L and the a5, respectively, along with a more anci...
Source: Molecular Immunology - June 4, 2019 Category: Allergy & Immunology Source Type: research

Carbon monoxide releasing molecule-2 protects against particulate matter-induced lung inflammation by inhibiting TLR2 and 4/ROS/NLRP3 inflammasome activation
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Chiang-Wen Lee, Miao-Ching Chi, Lee-Fen Hsu, Chuen-Mao Yang, Tsui-Hua Hsu, Chu-Chun Chuang, Wei-Ning Lin, Pei-Ming Chu, I-Ta LeeAbstractExposure to airborne particulate matter (PM) not only causes lung inflammation and chronic respiratory diseases, but also increases the incidence and mortality of cardiopulmonary diseases. The nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) inflammasome activation has been shown to play a critical role in the formation of many chronic disorders. On the other hand, carbon monoxide (CO) has be...
Source: Molecular Immunology - May 30, 2019 Category: Allergy & Immunology Source Type: research

Structural and immunological characterization of a new nucleotidyltransferase-like antigen from Paracoccidioides brasiliensis
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Juliana B. Coitinho, Mariana A.F. Costa, Eliza M. Melo, Elis A. Morais, Lorena G.A. de Andrade, Aline M. da Rocha, Mariana T.Q. de Magalhães, Denize C. Favaro, Lucas Bleicher, Enio R.P. Pedroso, Alfredo M. Goes, Ronaldo A.P. NagemAbstractPb27 antigen is an interesting alternative to immunological diagnosis of Paracoccidioidomycosis (PCM) and has demonstrated to be protective in experimental PCM. Its tertiary structure and possible function remained unknown till now. To study Pb27 at the atomic level, the recombinant protein was expressed...
Source: Molecular Immunology - May 18, 2019 Category: Allergy & Immunology Source Type: research

Fish-derived low molecular weight components modify bronchial epithelial barrier properties and release of pro-inflammatory cytokines
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Tanja Kalic, Isabella Ellinger, Sandip D. Kamath, Chiara Palladino, Vanessa Mayr, Angelika Tscheppe, Thimo Ruethers, Eva E. Waltl, Verena Niederberger, Nina Lengger, Christian Radauer, Christine Hafner, Andreas L. Lopata, Merima Bublin, Heimo BreitenederAbstractThe prevalence of fish allergy among fish-processing workers is higher than in the general population, possibly due to sensitization via inhalation and higher exposure. However, the response of the bronchial epithelium to fish allergens has never been explored. Parvalbumins (PVs) from bo...
Source: Molecular Immunology - May 18, 2019 Category: Allergy & Immunology Source Type: research

Phagocytosis of live and dead Escherichia coli and Staphylococcus aureus in human whole blood is markedly reduced by combined inhibition of C5aR1 and CD14
ConclusionC5aR1 inhibited phagocytosis-induced inflammation by live and dead E. coli and S. aureus. CD14 blockade potentiated the effect of C5aR1 blockade, thus attenuating inflammation. (Source: Molecular Immunology)
Source: Molecular Immunology - May 17, 2019 Category: Allergy & Immunology Source Type: research

The role of the light chain in the structure and binding activity of two cattle antibodies that neutralize bovine respiratory syncytial virus
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Jingshan Ren, Joanne E. Nettleship, Gemma Harris, William Mwangi, Nahid Rhaman, Clare Grant, Abhay Kotecha, Elizabeth Fry, Bryan Charleston, David I. Stuart, John Hammond, Raymond J. OwensAbstractCattle antibodies have unusually long CDR3 loops in their heavy chains (HCs), and limited light chain (LC) diversity, raising the question of whether these mask the effect of LC variation on antigen recognition. We have investigated the role of the LC in the structure and activity of two neutralizing cattle antibodies (B4 and B13) that bind the F prote...
Source: Molecular Immunology - May 15, 2019 Category: Allergy & Immunology Source Type: research

The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling
In this report, we demonstrate that Israa gene product regulates the expression of a pool of genes involved in T-cell activation and signaling. Real time PCR and GFP knock-in expression analysis showed that Israa is transcribed and expressed in the spleen mainly by CD3+CD8+ cells as well as in the thymus by CD3+ (DP and DN), CD4+SP and CD8+SP cells at different developmental stages. We also showed that Israa is downregulated in T-cells following activation of T-cell receptor. Using yeast two-hybrid analysis, we identified ELF1, a transcription factor involved in T-cell regulation, as an ISRAA-binding partner. Transcriptomi...
Source: Molecular Immunology - May 14, 2019 Category: Allergy & Immunology Source Type: research

Anti-inflammatory activity of intravenous immunoglobulin through scavenging of heme
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Marie Wiatr, Nicolas S. Merle, Idris Boudhabhay, Victoria Poillerat, Sofia Rossini, Maxime Lecerf, Srini V. Kaveri, Sébastian Lacroix-Desmazes, Lubka T. Roumenina, Jordan D. DimitrovAbstractTherapeutic intravenous immunoglobulin preparations (IVIg) are used for treatment of wide range of autoimmune and inflammatory diseases. Versatile mechanisms have been reported to contribute to the immunomodulatory effects of IVIg. Here we demonstrate that IVIg has a strong potential to inhibit pro-inflammatory effect of extracellular heme. Indeed, the ...
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

Identification of unique key genes and miRNAs in latent tuberculosis infection by network analysis
In this study, a systems biology approach was utilized to obtain numerous microarray data sets for mRNAs and microRNAs (miRNAs) expressed in the peripheral blood mononuclear cells (PBMCs) of TB patients and individuals with LTBI. Within these data sets, we identified the differentially expressed mRNAs and miRNAs and further investigated which differentially expressed genes and miRNAs were uniquely expressed during LTBI. The Database for Annotation, Visualization and Integrated Discovery (DAVID) was employed to analyze the functional annotations and pathway classifications of the identified genes. To further understand the ...
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

Persistent stimulation with Mycobacterium tuberculosis antigen impairs the proliferation and transcriptional program of hematopoietic cells in bone marrow
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Fei Li, Xun Liu, Hongxia Niu, Wei Lv, Xue Han, Yifan Zhang, Bingdong ZhuAbstractMycobacterium tuberculosis (M. tuberculosis) persistent infection might cause the dysfunction of hematopoiesis. To investigate whether M. tuberculosis persistent antigen stimulation impairs the proliferation and differentiation of hematopoietic stem and progenitor cells characterized as lineage− c-Kit+ (LK cells), C57BL/6 mice were primed with Mycobacterium bovis Bacillus Calmette-Guérin (BCG) and boosted with a cocktail of M. tuberculosis antigens ESAT...
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

Immunogenicity and protective efficacy of mucosal delivery of recombinant hcp of Campylobacter jejuni Type VI secretion system (T6SS) in chickens
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Ankita Singh, Khairun Nisaa, Sudipta Bhattacharyya, Amirul Islam MallickAbstractThe type VI secretion system (T6SS) has recently emerged as a new pattern of protein secretions in Campylobacter jejuni (C. jejuni). Within the T6SS cluster, hemolysin co-regulated protein (hcp) is considered as a hallmark of functional T6SS and holds key role in bacterial virulence. As poultry is the primary reservoir of C. jejuni and the major sources for human infection, we evaluated the capacity of recombinant hcp (rhcp) immunization in blocking C. jejuni coloniza...
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

Cellular immune response induced by surface immunogenic protein with AbISCO-100 adjuvant vaccination decreases group B Streptococcus vaginal colonization
In this study, we show that a vaccine based on a mixture of rSIP and AbISCO-100 adjuvant reduces GBS vaginal colonization in mice and induces antibodies with opsonophagocytic activities. Moreover, the passive transfer of sera and total T-cells from mice immunized with rSIP mixed with AbISCO-100 to unvaccinated mice decreases vaginal GBS colonization in an infected mouse. This is the first report of cellular immunity associated with rSIP-based vaccine testing in a mouse model of GBS infection. (Source: Molecular Immunology)
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

Long non-coding RNA: Classification, biogenesis and functions in blood cells
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Swati Dahariya, Indira Paddibhatla, Santosh Kumar, Sanjeev Raghuwanshi, Adithya Pallepati, Ravi Kumar GuttiAbstractWhile there exist some long non-coding RNAs (lncRNAs) that are structurally similar to mRNAs (capped, spliced, poly a tail), not all of the lncRNAs exhibit these features. Structurally, lncRNAs are classified under the regulatory non-coding RNAs category these lncRNA molecules operate as signals, decoys, guides, and scaffolds. In eukaryotes, lncRNAs are transcribed by RNA Polymerase II and RNA Polymerase III at several loci of the ...
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

S100A9 maintains myeloid-derived suppressor cells in chronic sepsis by inducing miR-21 and miR-181b
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Tuqa Alkhateeb, Ajinkya Kumbhare, Isatou Bah, Dima Youssef, Zhi Q. Yao, Charles E. McCall, Mohamed El GazzarAbstractMyeloid-derived suppressor cells (MDSC) expand during sepsis, suppress both innate and adaptive immunity, and promote chronic immunosuppression, which characterizes the late/chronic phase of sepsis. We previously reported that the transcription factors Stat3 and C/EBPβ synergize to induces the expression of microRNA (miR)-21 and miR-181b to promote MDSC expansion in a mouse model of polymicrobial sepsis that progresses from a...
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

In silico analysis of transmembrane protein 31 (TMEM31) antigen to design novel multiepitope peptide and DNA cancer vaccines against melanoma
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Ashkan Safavi, Amirhosein Kefayat, Ardavan Abiri, Elham Mahdevar, Amir Hossein Behnia, Fatemeh GhahremaniAbstractMultiepitope cancer vaccines are announcing themselves as the future of melanoma treatment. Herein, high immunogenic regions of transmembrane protein 31 (TMEM31) antigen were selected according to cytotoxic T lymphocytes’ (CTL) epitopes and major histocompatibility complex (MHC) binding affinity through in silico analyses. The 32–62, 77–105, and 125–165 residues of the TMEM31 were selected as the immunodominan...
Source: Molecular Immunology - May 11, 2019 Category: Allergy & Immunology Source Type: research

HLAIb worldwide genetic diversity: New HLA-H alleles and haplotype structure description
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Julien Paganini, Laurent Abi-Rached, Philippe Gouret, Pierre Pontarotti, Jacques Chiaroni, Julie Di CristofaroAbstractThe classical HLA class I genes (HLA Ia) were extensively studied because of their implication in clinical fields and anthropology. Less is known about worldwide genetic diversity and linkage disequilibrium for non-classical HLA class I genes (HLA Ib) and HLA pseudogenes. Notably, HLA-H, which is deleted in a fraction of the population, remains scarcely explored. The aims of this study were 1/ to get further insight into HLA-H g...
Source: Molecular Immunology - May 9, 2019 Category: Allergy & Immunology Source Type: research

UFL1 modulates NLRP3 inflammasome activation and protects against pyroptosis in LPS-stimulated bovine mammary epithelial cells
In this study, we observed an elevating NLRP3, Caspase-1 activation and IL-1β secretion in mammary tissue of cows with mastitis and LPS-stimulated BMECs, and the experimental results here demonstrated that UFL1 depletion aggravated the LPS-induced NLRP3, Caspase-1 and IL-1β expression. Overexpression of UFL1 significantly suppressed the expression of NLRP3, Caspase-1 and IL-1β in BMECs. In addition, the suppression of NLRP3 inflammasome activation by UFL1 was partly mediated through the regulation of NF-κB signaling and ROS production. Furthermore, UFL1 overexpression could alleviate NLRP3 inflammasome...
Source: Molecular Immunology - May 9, 2019 Category: Allergy & Immunology Source Type: research

LncRNA MALAT1 cessation antagonizes hypoxia/reoxygenation injury in hepatocytes by inhibiting apoptosis and inflammation via the HMGB1-TLR4 axis
In this study, elevation of MALAT1 expression was corroborated in human hepatocytes under hypoxia/reoxygenation (H/R)H/R condition. Of interest, depression of MALAT1 blunted H/R-inhibited cell viability, and counteracted lactate dehydrogenase (LDH) and malondialdehyde release. Additionally, MALAT1 cessation antagonized H/R-evoked cell apoptosis and caspase-3 activity. Simultaneously, the increased inflammatory reaction triggered by H/R stimulation was also abrogated following MALAT1 suppression by reducing pro-inflammatory cytokine transcripts and productions including IL-1β and TNF-α. Mechanistically, H/R expos...
Source: Molecular Immunology - May 9, 2019 Category: Allergy & Immunology Source Type: research

BpV(pic) confers neuroprotection by inhibiting M1 microglial polarization and MCP-1 expression in rat traumatic brain injury
In this study we aimed to investigate the neuroprotective role of bpV(pic) in rat TBI model. We found that injection of bpV(pic) significantly reduces brain edema and neurological dysfunction after TBI and this is mediated by AKT pathway. TBI is known to promote the M1 pro-inflammatory phenotype of microglial polarization and this effect is inhibited by bpV(pic) treatment which, instead promotes M2 microglial polarization in vivo and in vitro. We also found evidence of bpV(pic)-regulated neuroinflammation mediated by AKT activation and NF-κB p65 inhibition. BpV(pic) treatment also suppressed microglia in the peri-TBI...
Source: Molecular Immunology - May 9, 2019 Category: Allergy & Immunology Source Type: research

Chronic and low-level particulate matter exposure can sustainably mediate lung damage and alter CD4 T cells during acute lung injury
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Yusheng Li, Tiancao Dong, Xiaoping Jiang, Chunmei Wang, Ying Zhang, Yinzhen Li, Guizhen Zheng, Xiuhua Li, Jianwen Bai, Hongqiang LiAbstractParticulate matter (PM)2.5 is a common air pollutant known to induce damages in the respiratory, cardiovascular, and nervous systems. Previous study has shown that acute and high-level PM insult could significantly aggravate the severity of LPS-induced acute lung injury (ALI). However, humans typically experience more chronic and low-level PM, of which the effect on ALI is yet unclear. Here, we varied the co...
Source: Molecular Immunology - May 9, 2019 Category: Allergy & Immunology Source Type: research

Molecular structure, expression, and bioactivity of B-cell–activating factor of the TNF family (BAFF) and its receptor BAFF-R in cats (Felis catus)
In this study, the full-length cDNAs of cat BAFF (cBAFF) and BAFF-R (cBAFF-R) were amplified from the spleen by reverse transcription PCR. The open reading frame of cBAFF cDNA encodes a protein of 285 amino acids containing a predicted transmembrane domain and a furin protease cleavage site, similar to mammalian, avian, and reptile BAFFs. The cBAFF-R gene encodes a 189 amino acid protein. Real-time quantitative PCR analyses revealed that the two genes are predominantly expressed in the spleen. csBAFF, EGFP/csBAFF, and cBAFF-R were efficiently expressed in Escherichia coli BL21 (DE3), as confirmed by sodium dodecyl sulfate-...
Source: Molecular Immunology - May 9, 2019 Category: Allergy & Immunology Source Type: research

Identification and characterization of ecto-nucleoside triphosphate diphosphohydrolase 1 (CD39) involved in regulating extracellular ATP-mediated innate immune responses in Japanese flounder (Paralichthys olivaceus)
Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): Shuo Li, Xiaoli Chen, Nan Wang, Jiafang Li, Yu Feng, Jinsheng SunAbstractExtracellular adenosine triphosphate (eATP), released following inflammatory stimulation or infection, is a potent signaling molecule in activating innate immune responses in fish. However, the regulation of eATP-mediated innate immunity in fish remains unknown. Ecto-nucleoside triphosphate diphosphohydrolase 1 (CD39) is a critical molecular switch for controlling the ATP levels in the extracellular space. CD39 plays a key role in regulating eATP-activated innate immune re...
Source: Molecular Immunology - May 9, 2019 Category: Allergy & Immunology Source Type: research

The Schistosoma mansoni cyclophilin A epitope 107-121 induces a protective immune response against schistosomiasis
In this study, we characterized the cyclophilin A (SmCyp), initially described as Smp17.7, analyzed its allergenic potential using in vitro functional assays, and evaluated its ability to induce protection in mice when administered as an antigen using different vaccine formulations and strategies. Results indicated that SmCyp could be successfully expressed by mammalian cells and bacteria. The recombinant protein did not promote IgE-reporter system activation in vitro, demonstrating its probable safety for use in vaccine formulations. T and B-cell epitopes were predicted in the SmCyp sequence, with two of them located with...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Bcells and their regulatory functions during Tuberculosis: Latency and active disease
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Andre G. LoxtonAbstractTuberculosis (TB) is a global epidemic with devastating consequences. Emerging evidence suggests that B-cells have the ability to modulate the immune response and understanding these roles during Mycobacterium tuberculosis (M.tb) infection can help to find new strategies to treat TB. The immune system of individuals with pulmonary TB form granulomas in the lung which controls the infection by inhibiting the M.tb growth and acts as a physical barrier. Thereafter, surviving M.tb become dormant and in most cases the host&rsquo...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Regulation of CD11b by HIF-1α and the STAT3 signaling pathway contributes to the immunosuppressive function of B cells in inflammatory bowel disease
In conclusion, our study demonstrated the key function of the hypoxia-associated transcription factor HIF-1α together with p-STAT3 in driving CD11b transcription in B cells and controlling B cell’s protective activity in experimental inflammatory bowel disease (IBD). (Source: Molecular Immunology)
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Pollen food allergy syndrome to tomato in mountain cedar pollen hypersensitivity
ConclusionThis is the first report of a PFAS in patients with mountain cedar pollinosis. Sensitivity to tomato, banana, and apple should be considered in cedar-sensitive patients. (Source: Molecular Immunology)
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Changes in apoptosis, proliferation and T lymphocyte subtype on thymic cells of SPF chickens infected with reticuloendotheliosis virus
In this study, a hundred and twenty one-day old SPF chickens were randomly divided into control groups (group C) and a REV infection groups (group I). The chickens of group I received intraperitoneal injections of REV with 104.62/0.1 ml TCID50. On day 14, 21, 28 and 35 post-inoculation, the chickens of C group and I group were sacrificed by cardiac puncture blood collection, and the thymic lymphocytes was sterile collected. The proliferation ability of lymphocytes was tested by Cell Counting Kit-8. Flow cytometry was performed to detect apoptosis, cell cycle stage and the change in T cell subtype. The RNA genome cop...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Involvement of multiple transcription factors in regulation of IL-β-induced MCP-1 expression in alveolar type II epithelial cells
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Chunguang Yan, Bingyu Li, Xiufang Liu, Chunming Deng, Rentian Cai, Yanfei Shen, Huifang TangAbstractDuring acute lung injury, a large number of monocytes are recruited into the pulmonary tissue, which is mainly mediated by local production of monocyte chemotactic protein 1 (MCP-1). As an essential component of the lung tissues, alveolar type II epithelial cells are one of the major sources of MCP-1. Therefore, uncovering the mechanism whereby MCP-1 production is regulated in the alveolar type II cells will provide a pivotal theoretical basis for ...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

A new strategy for enhancing antitumor immune response using dendritic cells loaded with chemo-resistant cancer stem-like cells in experimental mice model
This study opens the field for combining CSC-targeted immunotherapy with repeated low doses chemotherapy as an effective strategy to improve anticancer immune responses. (Source: Molecular Immunology)
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Phenothiazine antipsychotics exhibit dual properties in pseudo-allergic reactions: Activating MRGPRX2 and inhibiting the H1 receptor
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Yajing Hou, Delu Che, Di Wei, Cheng Wang, Yitong Xie, Kaining Zhang, Jiao Cao, Jia Fu, Nan Zhou, Huaizhen HeAbstractPhenothiazines are a class of antipsychotics that share the same tricyclic structure and are widely used in clinical settings. Adverse reactions from these drugs, however, have been regularly reported, with allergic skin reactions noted in some cases. Nevertheless, the mechanisms underlying anaphylaxis by these drugs have not been described. In the present study, we found that phenothiazine antipsychotics increased calcium mobilizat...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

A critical challenge in the development of antibody: Selecting the appropriate fragment of the target protein as an antigen based on various epitopes or similar structure
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Mahboubeh Razzaqi, Mohammad Javad Rasaee, Maliheh PaknejadAbstractThe main challenge in the development of antibody is to select the appropriate antigen particularly when a truncated protein is used for immunization or as vaccine antigen. In previous studies, fragment selection was mainly based on epitopes and less often on the structure. Fewer studies have paid attention to the prediction of the truncated protein 3D structure and retained its similarity in the native and truncated proteins. Here we used in silico analysis to select two fragments...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Modulation of cathelicidins, IFNβ and TNFα by bovine alpha-herpesviruses is dependent on the stage of the infectious cycle
This study provides an initial pilot analysis of factors that might be implicated in alpha-herpesvirus infection of the bovine respiratory system. (Source: Molecular Immunology)
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

A non-pathogenic Leishmania tarentolae vector based- HCV polytope DNA vaccine elicits potent and long lasting Th1 and CTL responses in BALB/c mice model
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Samimi-Rad KatayounAbstractDespite successful anti-viral (DAAs) treatment of Hepatitis C virus (HCV) infection, recent data indicated the need for an effective vaccine. Preexisting anti-vector immunity is an obstacle for application of live vectors for antigen delivery and development of effective T-cell based HCV vaccines. Herein, we report construction of recombinant Leishmania tarentolae, a lizard (non-human) parasite, expressing an HCV polytope DNA, PT-NT(gp96), encoding for several immunogenic HCV epitopes and evaluation of its immunogenicit...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

The role of microtubules in the immune system and as potential targets for gut-based immunotherapy
Publication date: July 2019Source: Molecular Immunology, Volume 111Author(s): Tahel Ilan-Ber, Yaron IlanAbstractMicrotubules (MTs) are tubular polymers of tubulin that are highly dynamic and found throughout the cytoplasm. MTs are involved in maintaining cell structure and, together with microfilaments and intermediate filaments, form the cytoskeleton. Recent findings on MT structure and function contributed to the understanding of their potential role as players in the innate and adaptive immune systems. Additionally, studies suggest an essential role for these cellular structures in the gut. Here, we review recent data o...
Source: Molecular Immunology - May 8, 2019 Category: Allergy & Immunology Source Type: research

Adaptive immune stimuli altered the cargo proteins of exosomes released by supraneural myeloid body cells in Lampetra japonica
In this study, by means of ultrafiltration followed by size exclusion chromatography, exosomes are purified from conditioned growth medium of the primary supraneural myeloid body cells (SMB cells) in LPS-immunized and control Lampetra japonica (Japanese lamprey). The proteomic profiling and qualitative comparison are performed between protein components enriched in exosomes released by SMB cells under quiescent and activated conditions. Our results demonstrate that SMB cells can release exosomes with well-defined characteristics comparable to their mammalian counterparts on their size, morphology and protein markers, which...
Source: Molecular Immunology - April 23, 2019 Category: Allergy & Immunology Source Type: research

Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages
In conclusion, rhHsp70 provoked pro-inflammatory effects and could also modulate inflammatory response to CSE on protein and gene expression levels in THP-1 cells and MDMs, which suggests that eHsp70 might be implicated in systemic inflammation induced by cigarette smoke. (Source: Molecular Immunology)
Source: Molecular Immunology - April 13, 2019 Category: Allergy & Immunology Source Type: research