Genetic diversity of the HLA system in human populations from the Sierra (Andean), Oriente (Amazonian) and Costa (Coastal) regions of Ecuador.
A, Krause J, Grijalva M Abstract We studied HLA class I (HLA-A, -B) and class II (HLA-DRB1, -DQB1) alleles by PCR-SSP based typing in a total of 1101 Ecuadorian individuals from three regions of the country, the Coastal region, the Andean region, and the Amazonian region, to obtain information regarding allelic and haplotypic frequencies and their linkage disequilibrium. We find that the most frequent HLA haplotypes with significant linkage disequilibrium in those populations are HLA-A∗24∼B∗35∼DRB1∗04∼DQB1∗03:02, A∗02∼B∗35∼DRB1∗04∼DQB1&lowast...
Source: Human Immunology - June 13, 2018 Category: Allergy & Immunology Authors: Galarza JM, Barquera R, Álvarez AMT, Hernández Zaragoza DI, Sevilla GP, Tamayo A, Pérez M, Dávila D, Birnberg L, Alonzo VA, Krause J, Grijalva M Tags: Hum Immunol Source Type: research

Next Generation Sequencing in HLA Haplotype distribution among Telugu Speaking Population from Andhra Pradesh, India.
Abstract HLA A: B:C:DRB1:DQB1 allele and haplotype frequencies were determined among India, Andhra Pradesh, Telugu speaking population from South India by Next Generation Sequencing. 180 bone marrow registry donors and 6 cord blood units from Jeevan Stem Cell Foundation (part of Be The Cure Registry), Chennai, Tamilnadu state were included in the study. PMID: 29890180 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - June 8, 2018 Category: Allergy & Immunology Authors: Seshasubramanian V, Manisekar NK, Sathishkannan AD, Naganathan C, Narayan S Tags: Hum Immunol Source Type: research

Prevalence and risk factors of antibodies to human leukocyte antigens in haploidentical stem cell transplantation candidates: A multi-center study.
Abstract We investigated the prevalence of and risk factors for antibodies to HLA in 1,663 haploidentical transplant candidates. Among these cases, 349 (21.0%) showed positive panel-reactive antibody (PRA) either for class I or class II HLA. Multivariate analysis showed the following: i) risk factors associated with the prevalence of PRA either for class I or class II HLA were female gender (P=0.018), prior transfusions (P
Source: Human Immunology - June 8, 2018 Category: Allergy & Immunology Authors: Huo MR, Xu YJ, Zhai SZ, Lv M, Wang Y, Cao LQ, Xu LP, Zhang XH, Chen H, Chen YH, Wang FR, Han W, Sun YQ, Yan CH, Tang FF, Mo XD, Zhao MF, Liu KY, Huang XJ, Chang YJ Tags: Hum Immunol Source Type: research

IL-1 β induces expression of costimulatory molecules and cytokines but not immune feedback regulators in dendritic cells.
IL-1β induces expression of costimulatory molecules and cytokines but not immune feedback regulators in dendritic cells. Hum Immunol. 2018 Jun 07;: Authors: Michelini S, Sarajlic M, Duschl A, Horejs-Hoeck J Abstract Dendritic cells play an important role in the initiation of immune reactions. Due to their high capacity to prime T-cell responses, the activation of dendritic cells must be tightly controlled. Because Interleukin-1β (IL-1β) is a key player in autoinflammatory diseases, we compared the ability of IL-1β to activate human dendritic cells and induce immune-regulatory molec...
Source: Human Immunology - June 7, 2018 Category: Allergy & Immunology Authors: Michelini S, Sarajlic M, Duschl A, Horejs-Hoeck J Tags: Hum Immunol Source Type: research

Patterns of non-ARD variation in more than 300 full-length HLA-DPB1 alleles.
ml;fl G Abstract Our understanding of sequence variation in the HLA-DPB1 gene is largely restricted to the hypervariable antigen recognition domain (ARD) encoded by exon 2. Here, we employed a redundant sequencing strategy combining long-read and short-read data to accurately phase and characterise in full length the majority of common and well-documented (CWD) DPB1 alleles as well as alleles with an observed frequency of at least 0.0006% in our predominantly European sample set. We generated 664 DPB1 sequences, comprising 279 distinct allelic variants. This allows us to present the, to date, most comprehensive an...
Source: Human Immunology - June 4, 2018 Category: Allergy & Immunology Authors: Klasberg S, Lang K, Günther M, Schober G, Massalski C, Schmidt AH, Lange V, Schöfl G Tags: Hum Immunol Source Type: research

IGHG, IGKC, and FCGR Genes and Endogenous Antibody Responses to GARP in Patients with Breast Cancer and Matched Controls.
Abstract Glycoprotein-A repetitions predominant (GARP) is a transmembrane protein that is highly expressed in breast cancer. Its overexpression correlates with worse survival, and antibodies to GARP appear to play a protective role in a mouse model. No large-scale studies of immunity to GARP in humans have yet been undertaken. In this investigation, using a large multiethnic cohort (1738 subjects), we aimed to determine whether the magnitude of anti-GARP antibody responsiveness was significantly different in patients with breast cancer from that in matched healthy controls. We also investigated whether the allelic...
Source: Human Immunology - June 4, 2018 Category: Allergy & Immunology Authors: Pandey JP, Namboodiri AM, Armeson KE, Iwasaki M, Kasuga Y, Hamada GS, Tsugane S Tags: Hum Immunol Source Type: research

The First Free Africans in America: HLA Study in San Basilio de Palenque (Colombia).
era C Abstract Original San Basilio de Palenque population (North Colombia) fled from Spanish traders that carried them as slaves and they funded in nearby Maria mountains a fortified town (Palenque). They started helping new Africans brought as slaves to flee and join them. Most of them spoke a Bantu-Congo language and nowadays they speak the only one extant Bantu-Spanish Creole language. Spanish Crown were forced to issue a decree declaring them free (1691 AD), more than 100 years before than Haiti Republic existed. HLA-A, -B, -DRB1 and -DQB1 alleles were studied and further computer procedures were performed wi...
Source: Human Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Arnaiz-Villena A, Juarez I, Palacio-Gruber J, Muñiz E, Campos C, Martinez-Laso J, Nieto J, Lopez-Nares A, Martin-Villa JM, Silvera C Tags: Hum Immunol Source Type: research

Improving the performance of virtual crossmatch results by correlating with nationally-performed physical crossmatches: obtaining additional value from proficiency testing activities.
CONCLUSION: Review of locally-generated MFI results as part of participating in proficiency testing allow for predictability of crossmatch results against other laboratories, providing a replicable model for other participating centers. PMID: 29864460 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - June 1, 2018 Category: Allergy & Immunology Authors: Wrenn SM, Marroquin CE, Hain DS, Harm S, Pineda J, Hammond PB, Shah DH, Hillyard SE, Fung M Tags: Hum Immunol Source Type: research

A Sarcoidosis Clinician's Perspective of MHC Functional Elements Outside the Antigen Binding Site.
Abstract Sarcoidosis is a multisystem granulomatous disease of unknown cause. Evidence supports an integral role for interactions at the MHC binding site in the development of sarcoidosis. However, despite this evidence, there are clinical data that suggest that additional mechanisms are involved in the immunopathogenesis of this disease. This manuscript provides a brief clinical description of sarcoidosis, and a clinician's perspective of the immunopathogenesis of sarcoidosis in terms of the MHC binding site, MHC functional elements beyond the binding site, and other possible alternative mechanisms. Input from cl...
Source: Human Immunology - May 30, 2018 Category: Allergy & Immunology Authors: Judson MA Tags: Hum Immunol Source Type: research

10-Year Experience with HLA-G in Heart Transplantation.
Abstract The Human Leukocyte Antigen-G (HLA-G) is a MHC-class Ib molecule with robust immunomodulatory properties; in transplant, it inhibits cytotoxic activity of immune cells and thus has a pivotal role in protecting the allograft from immune attack. The present review details a 10-year experience investigating the influence of HLA-G on heart transplantation, allograft rejection and cardiac allograft vasculopathy development. Exploration of HLA-G in transplantation began with the initial findings of its increased expression in allograft hearts. Since then, HLA-G has been recognized as an important factor in tran...
Source: Human Immunology - May 30, 2018 Category: Allergy & Immunology Authors: Lazarte J, Adamson MB, Tumiati LC, Delgado DH Tags: Hum Immunol Source Type: research

Analysis of programmed death-ligand 1 expression in primary normal human dermal fibroblasts after DNA damage.
Abstract Programmed cell death-1 (PD-1) and its ligand (programmed death-ligand 1, PD-L1) are key factors that regulate a cytotoxic immune reaction. Anti-PD-1 therapy provides significant clinical benefits for patients with cancer, even those with advanced-stage cancer. We have recently demonstrated that DNA damage signaling from DNA double-strand breaks (DSBs) promotes PD-L1 upregulation in cancer cells. In the present study, we aimed to investigate PD-L1 expression in primary normal human dermal fibroblasts (NHDFs) in response to DSBs. We demonstrated that PD-L1 expression in NHDFs is not upregulated after ioniz...
Source: Human Immunology - May 30, 2018 Category: Allergy & Immunology Authors: Hagiwara Y, Sato H, Permata TBM, Niimi A, Yamauchi M, Oike T, Nakano T, Shibata A Tags: Hum Immunol Source Type: research

CD8dim but not CD8bright cells positive to CD56 dominantly express KIR and are cytotoxic during visceral leishmaniasis.
This study reports a structural and functional heterogeneity of CD8+CD56+NKT cells, which usually decrease quantitatively during visceral leishmaniasis. Based on fluorescence intensity of CD8 receptors on CD56+NKT cells, two populations of CD8+CD56+NKT cells have been identified. These cells were recognized as CD8dimCD56+NKT and CD8brightCD56+NKT cells. We further analyzed the functional nature of CD8dim and CD8bright positive CD56+NKT cells. In comparison to CD8brightCD56+NKT cells, a significantly higher percentage of CD8dimCD56+NKT cells expressed KIR during VL. The percentage of CD8dimCD56+NKT cells expressing KIR was ...
Source: Human Immunology - May 26, 2018 Category: Allergy & Immunology Authors: Kumari S, Shivam P, Hansa J, Jamal F, Kumar Singh M, Bimal S, Narayan S, Pandey K, Ravi Das VN, Das P, Singh SK Tags: Hum Immunol Source Type: research

Heightened expression of HLA-DQB1 and HLA-DQB2 in pre-implantation biopsies predicts poor late kidney graft function.
ima M Abstract BACKGROUND: Accurate pre-transplant prediction of late graft function remains an unmet need in kidney transplantation. The aim of this study was to evaluate HLA genes expression levels in pre-implantation biopsies (PIB) of deceased donor kidneys as markers for long-term graft outcome. METHODS: HLA genes expression analysis was initially performed using microarray data of 53 PIB, previously generated by our laboratory. The validation analysis was performed by real-time PCR in 116 PIB from an independent cohort. RESULTS: The microarray data showed association between high expression levels o...
Source: Human Immunology - May 22, 2018 Category: Allergy & Immunology Authors: Mine KL, Tedesco-Silva H, Mourão TB, Campos EF, Salzedas LA, Aguiar B, Felipe CR, Medina-Pestana JO, Gerbase-DeLima M Tags: Hum Immunol Source Type: research

Function of T regulatory type 1 cells is down-regulated and is associated with the clinical presentation of coronary artery disease.
Abstract T regulatory type 1 (Tr1) cells can promote tolerance and suppress inflammation. Atherosclerosis may be induced by the proinflammatory activation of cells in the vasculature and the immune system. Hence, we wondered whether defects in Tr1 function were a contributing factor to coronary artery disease (CAD). Data showed that the frequency of IL-10+ Tr1 cells was significantly lower in CAD patients than in controls. Compared to healthy controls, Tr1 cells from CAD patients presented lower CTLA-4 but higher PD-1 expression, in addition to lower IL-10 secretion. When co-incubated with Tconv cells, the CD4+CD4...
Source: Human Immunology - May 3, 2018 Category: Allergy & Immunology Authors: Zhu Z, Ye J, Ma Y, Hua P, Huang Y, Fu X, Li D, Yuan M, Xia Z Tags: Hum Immunol Source Type: research

CCR5 Δ32 - a piece of protection in the inflammatory puzzle of multiple sclerosis susceptibility.
CONCLUSION: The CCR5Δ32 deletion is a protective factor against the development and progression of MS in European-derived Brazilian patients. PMID: 29729320 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - May 2, 2018 Category: Allergy & Immunology Authors: Troncoso LL, Pontillo A, de Oliveira EML, Finkelszteijn A, Schneider S, Chies JAB Tags: Hum Immunol Source Type: research

Study of Colombia North Wiwa El Encanto Amerindians HLA- Genes: Pacific Islanders Relatedness.
in-Villa M, Silvera C Abstract We have studied Wiwa/Sanja Amerindians HLA-A,-B,-C.-DRB1 and DQB1 allele frequencies and extended haplotypes in 52 unrelated individuals from "El Encanto" town at Guanachacariverside.Highfrequency alleles were in general present in other Amerindian populetions.Also,three extended haplotypes and eight ones were respectively both "new found" and already described in Amerindians from North,Central and South America,including Lakota-Sioux,Mayas,Teeneks,Quechua and Aymaras.Analyses of HLA-A∗24:02 and -C∗01:02 Wiwa high frequency alleles suggested a specif...
Source: Human Immunology - May 2, 2018 Category: Allergy & Immunology Authors: Arnaiz-Villena A, Palacio-Grüber J, Muñiz E, Hernández E, Bayona B, Campos C, Nuñez J, AdrianLopez-Nares, Martin-Villa M, Silvera C Tags: Hum Immunol Source Type: research

B cell MHC Class II Signaling: a Story of Life and Death.
Abstract MHC class II regulates B cell activation, proliferation, and differentiation during cognate B cell -T cell interaction. This is, in part, due to the MHC class II signaling in B cells. Activation of MHC Class II in human B cells or "primed" murine B cells leads to tyrosine phosphorylation, calcium mobilization, AKT, ERK, JNK activation. In addition, crosslinking MHC class II with monoclonal Abs kill malignant human B cells. Several humanized anti-HLA-DR/MHC class II monoclonal Abs entered clinical trials for lymphoma/leukemia and MHC class II-expressing melanomas. Mechanistically, MHC class II is...
Source: Human Immunology - April 28, 2018 Category: Allergy & Immunology Authors: Katikaneni DS, Jin L Tags: Hum Immunol Source Type: research

Genetic screening of male patients with primary hypogammaglobulinemia can guide diagnosis and clinical management.
In conclusion, our results advocate to include SH2D1A and BTK in newly developed targeted NGS genetic testing, to contribute to providing the most appropriate medical treatment and genetic counselling. PMID: 29709555 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 27, 2018 Category: Allergy & Immunology Authors: Vince N, Mouillot G, Malphettes M, Limou S, Boutboul D, Guignet A, Bertrand V, Pellet P, Gourraud PA, Debré P, Oksenhendler E, Théodorou I, Fieschi C, DEFI Study Group Tags: Hum Immunol Source Type: research

HLA -A, -C, -B, -DRB1, -DQB1 and -DPB1 allele and haplotype frequencies in 4514 healthy Norwegians.
We report HLA-A, -C, -B, -DRB1, -DQB1 and -DPB1 allele frequencies and estimated haplotype frequencies from 4514 healthy Norwegians who volunteered to participate in the Norwegian Bone Marrow Donor Registry. HLA genotyping was conducted on a Next Generation Sequencing platform. Data were analyzed using Arlequin and Pypop software. No significant deviations from Hardy-Weinberg Equilibrium were noted at any of the loci studied. We discuss the representability for the Norwegian population and argue that the presented HLA data could serve as a Norwegian reference panel. PMID: 29684411 [PubMed - as supplied by publisher] (...
Source: Human Immunology - April 20, 2018 Category: Allergy & Immunology Authors: Lande A, Andersen I, Egeland T, Lie BA, Viken MK Tags: Hum Immunol Source Type: research

No Association of Il2, Il4, Il6, Tnf, and Ifng Gene Polymorphisms was Found with Taenia Solium Human Infection or Neurocysticercosis Severity In a Family-Based Study.
This study evaluated the association of cytokine single nucleotide polymorphisms (SNPs) with susceptibility to infection and disease severity in NC patients. Blood samples from 92 NC cases and their parents (trios) were genotyped for SNPs in five cytokines relevant for the immune response: IL4 (-589C/T), IL6 (-174C/G), IFNG (+874T/A), TNF (-238G/A), and IL2 (-330G/T). Specific DNA fragments were amplified by the polymerase chain reaction, using the 5'-nuclease Taqman assay on a 7500 platform, allowing the detection of the polymorphism genotypes. No association between the polymorphisms evaluated neither with susceptibility...
Source: Human Immunology - April 20, 2018 Category: Allergy & Immunology Authors: Fleury A, Alaez C, Dessein A, Rosetti M, Saenz B, Hernández M, Bobes RJ, Aquino R, Sciutto E, Gorodezky C, Fragoso G Tags: Hum Immunol Source Type: research

Continue to Focus Clinical Decision Making on the Antigen Recognition Domain for the Present.
Abstract Next generation DNA sequencing has facilitated the routine characterization of complete HLA gene sequences. These data complement structural and functional studies of HLA elements encoded outside of the exons specifying the antigen recognition domain. This commentary is focused on evaluating whether the interpretation of HLA clinical typing results should expand the region of the HLA gene considered in the assignment from the exon(s) encoding the antigen recognition domain to the full gene sequence. Our recommendation is that, at present, there is insufficient data to support considering variation in the ...
Source: Human Immunology - April 20, 2018 Category: Allergy & Immunology Authors: Hurley CK, Ng J Tags: Hum Immunol Source Type: research

Limited impact of pre-existing donor specific HLA-antibodies (DSA) on long term allograft survival after first adult liver transplantation.
Abstract The relevance of pre-existing HLA-antibodies (HLA-Ab) in liver transplantation (LTx) is controversial. While livers are allocated without HLA match or preoperative crossmatch testing, several studies point to an impact of donor specific antibodies (DSA) for acute rejection, bile duct complications or even graft loss. To investigate the role of DSA in long term liver allograft survival we analysed 177 pre transplant sera of first LTx patients with luminex single antigen technology defining a MFI of>1500 as positive. The analyses were done retrospectively, and the DSA results had no impact on graft accep...
Source: Human Immunology - April 15, 2018 Category: Allergy & Immunology Authors: Koch M, Marget M, Sterneck M, Fischer L, Thude H, Nashan B Tags: Hum Immunol Source Type: research

Nomenclature for factors of the HLA system, update February 2018.
PMID: 29660448 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 13, 2018 Category: Allergy & Immunology Authors: Marsh SGE, WHO Nomenclature Committee for Factors of the HLA System. Tags: Hum Immunol Source Type: research

Nomenclature for factors of the HLA system, update January 2018.
PMID: 29660449 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 13, 2018 Category: Allergy & Immunology Authors: Marsh SGE, WHO Nomenclature Committee for Factors of the HLA System. Tags: Hum Immunol Source Type: research

Nomenclature for factors of the HLA system, update March 2018.
PMID: 29660450 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 13, 2018 Category: Allergy & Immunology Authors: Marsh SGE, WHO Nomenclature Committee for Factors of the HLA System. Tags: Hum Immunol Source Type: research

Does Matching for SNPs in the MHC Gamma Block in 10/10 HLA-Matched Unrelated Donor-Recipient Pairs Undergoing Allogeneic Stem Cell Transplant Improve Outcomes?
CONCLUSIONS: In our single institution study, gamma-type matching status was not associated with outcomes of adult ASCT recipients. PMID: 29660451 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 13, 2018 Category: Allergy & Immunology Authors: Moyer AM, Hashmi SK, Kroning C, De Goey SR, Patnaik M, Litzow M, Gastineau DA, Hogan WJ, Jacob EK, Kreuter JD, Wakefield LL, Gandhi MJ Tags: Hum Immunol Source Type: research

Simulation of non-inherited maternal antigens acceptable HLA mismatches to increase the chance of matched cord blood units: Hong Kong's experience.
Abstract In Cord blood transplantation (CBT), the non-inherited maternal antigen (NIMA) virtual six HLA matched CB is found to have similar outcomes to six HLA inherited matched CB. Such virtual HLA matched CB units can be generated by substituting the inherited alleles with one to three NIMAs. In Hong Kong Cord Blood Bank, CB units have no NIMA defined. 100 CB samples were collected with NIMA defined. Retrospective searches of Hong Kong patients (n = 520) were matched against the inherited and virtual HLA phenotypes of NIMA CB file. One to three NIMA matches was analyzed, virtual six HLA matches were identified f...
Source: Human Immunology - April 13, 2018 Category: Allergy & Immunology Authors: Kwok J, Guo M, Yang W, Ip P, Lee CK, Lu L, Chan GCF, Middleton D, van der Zanden HGM Tags: Hum Immunol Source Type: research

Circulating Histocompatibility Antigen (HLA) Gene Products May Help Differentiate Benign from Malignant Indeterminate Pulmonary Lesions.
CONCLUSIONS: Soluble HLA molecules may have diagnostic value for early-stage NSCLC. Validation studies are currently underway using sera from a lung cancer screening cohort. PMID: 29656111 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 12, 2018 Category: Allergy & Immunology Authors: Kanangat S, Seder CW, Pergande MR, Lobato GC, Fhied CL, Raouf MF, Liptay MJ, Borgia JA Tags: Hum Immunol Source Type: research

Influence of immunosuppressive drugs on the CD30 molecule in kidney transplanted patients.
CONCLUSIONS: Overall, sCD30 levels are lower in SRL-treated patients, but the association between increased sCD30 levels and IF/TA at month-24 post-transplantation is stronger in SRL than in TAC-treated patients. PMID: 29656112 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 12, 2018 Category: Allergy & Immunology Authors: Grenzi PC, Campos ÉF, Tedesco-Silva H, Felipe CR, Soares MF, Medina-Pestana J, Hansen HP, Gerbase-DeLima M Tags: Hum Immunol Source Type: research

Donor Cell Chimerism Evaluation in Cerebrospinal Fluid by Short Tandem Repeat Analysis Following Allogeneic Hematopoietic Stem Cell Transplantation.
Abstract Cerebrospinal fluid (CSF) cell count, morphology and flow cytometric evaluations are used to investigate central nervous system (CNS) involvement in leukemia/lymphoma. We performed CSF short tandem repeat (STR) analysis to monitor CSF chimerism status and evaluate for disease involvement in 11 asymptomatic pediatric allogeneic hematopoietic stem cell transplantation (HSCT) recipients with hemato-lymphoid neoplasms and high risk for or history of CNS involvement. Eighteen (64%) of the samples with median CSF cell count of 1/mm3 with 90% lymphocytes gave conclusive STR results, suggesting that this DNA-base...
Source: Human Immunology - April 11, 2018 Category: Allergy & Immunology Authors: Spruit JL, Fagoaga OR, Savaşan S Tags: Hum Immunol Source Type: research

Peripheral blood immune response-related gene analysis for evaluating the potential risk of chronic antibody-mediated rejection.
This study aimed to elucidate the clinical relevance of immune-related gene expression in peripheral blood of kidney transplant recipients. The expression levels of fourteen key molecules (Foxp3, CTLA-4, CCR7, TGF-β, IGLL-1, IL-10, ITCH, CBLB, Bcl-6, CXCR5, granzyme B, CIITA, Baff, TOAG-1/TCAIM) related to regulatory/cytotoxic function of immune cells were compared in 93 patients, which were divided into Groups A (clinical cAMR with dn DSA, n=16), B (subclinical cAMR with dn DSA, n=17), C (negative cAMR with dn DSA, n=21) and D (stable function without dn DSA, n=39). CIITA mRNA expression levels in groups B and C were...
Source: Human Immunology - March 31, 2018 Category: Allergy & Immunology Authors: Yamamoto T, Iwasaki K, Murotani K, Takeda A, Futamura K, Okada M, Tsujita M, Hiramitsu T, Goto N, Narumi S, Watarai Y, Morozumi K, Uchida K, Kobayashi T Tags: Hum Immunol Source Type: research

Characterization of peripheral blood TCR repertoire in patients with ankylosing spondylitis by high-throughput sequencing.
Abstract Ankylosing spondylitis (AS) is a chronic and progressive autoimmune disease affecting the invasion of the spine, sacroiliac joints and peripheral joints. T cells play a vital role in the underlying pathogenesis of AS, which mediated autoimmune and inflammatory responses via specific recognition of autoantigen peptides presented by susceptibility HLA. Antigen-specific T cells triggered by HLA/antigen complexes will undergo a massive expansion that forming an uneven T cell repertoire. To enhance our understanding of T-cell-mediated autoimmune in AS, we applied TCR β chains high-throughput sequencing to...
Source: Human Immunology - March 31, 2018 Category: Allergy & Immunology Authors: Cui JH, Jin YB, Lin KR, Xiao P, Chen XP, Pan YM, Lin W, Wu ZC, Guo DM, Mao XF, Zhang CL, Lian WL, Luo W Tags: Hum Immunol Source Type: research

HLA-inferred extended haplotype disparity level is more relevant then the level of HLA mismatch alone for the patients survival and GvHD in T cell-replate hematopoietic stem cell transplantation from unrelated donor.
achowiak J, Czyz A, Gil L, Lojko-Dankowska A, Komarnicki M, Bieniaszewska M, Kucharska A, Hellmann A, Gronkowska A, Jedrzejczak WW, Markiewicz M, Koclega A, Kyrcz-Krzemien S, Mielcarek M, Kalwak K, Styczynski J, Wysocki M, Drabko K, Wójcik B, Kowalczyk J, Gozdzik J, Pawliczak D, Gwozdowicz S, Dziopa J, Szlendak U, Witkowska A, Zubala M, Gawron A, Warzocha K, Lange A Abstract Serious risks in unrelated hematopoietic stem cell transplantation (HSCT) including graft versus host disease (GvHD) and mortality are associated with HLA disparity between donor and recipient. The increased risks might be dependent on ...
Source: Human Immunology - March 29, 2018 Category: Allergy & Immunology Authors: Nowak J, Nestorowicz K, Graczyk-Pol E, Mika-Witkowska R, Rogatko-Koros M, Jaskula E, Koscinska K, Madej S, Tomaszewska A, Nasilowska-Adamska B, Szczepinski A, Halaburda K, Dybko J, Kuliczkowski K, Czerw T, Giebel S, Holowiecki J, Baranska M, Pieczonka A, Tags: Hum Immunol Source Type: research

HLA-G mediated immune regulation is impaired by a single amino acid exchange in the alpha 2 domain.
g C Abstract The trade-off from HLA class I expression to HLA-G expression support the immune evasion of malignant cells. The essential role of the virtually invariant HLA-G in immune tolerance, tumor immunology and its expression frequency in immune privileged tissues is known; however the specific importance of allelic subtypes in immune responses is still not well understood. HLA-G∗01:01, ∗01:03 and ∗01:04 are the most prevalent allelic variants differing at residues 31 and 110, respectively. In cytotoxicity assays applying K562 cells transduced with the HLA-G variants as targets and NK cel...
Source: Human Immunology - March 29, 2018 Category: Allergy & Immunology Authors: Celik AA, Simper GS, Huyton T, Blasczyk R, Bade-Döding C Tags: Hum Immunol Source Type: research

Lack of evidence of association between ifng and il28b polymorphisms and quantiferon-cmv test results in seropositive transplant patients.
LACK OF EVIDENCE OF ASSOCIATION BETWEEN IFNG AND IL28B POLYMORPHISMS AND QUANTIFERON-CMV TEST RESULTS IN SEROPOSITIVE TRANSPLANT PATIENTS. Hum Immunol. 2018 Mar 29;: Authors: Aguado R, Páez-Vega A, Agüera ML, Montejo M, Guirado L, Fortún J, Suárez-Benjumea A, Len O, Fariñas MC, de Gracia C, Hernández D, Cobos-Ceballos MJ, Torre-Cisneros J, Cantisán S, Spanish Network for Research in Infectious Diseases, Spanish Renal Disease Network. Spanish Transplantation Infection Study Group (GESITRA) of the Spanish Society of Infectious Diseases, Clinical Microbiology (SEIMC)...
Source: Human Immunology - March 29, 2018 Category: Allergy & Immunology Authors: Aguado R, Páez-Vega A, Agüera ML, Montejo M, Guirado L, Fortún J, Suárez-Benjumea A, Len O, Fariñas MC, de Gracia C, Hernández D, Cobos-Ceballos MJ, Torre-Cisneros J, Cantisán S, Spanish Network for Research in Infectious Diseases, Spanish Renal Di Tags: Hum Immunol Source Type: research

Gene variants in the NF-KB pathway (NFKB1, NFKBIA, NFKBIZ) and their association with type 2 diabetes and impaired renal function.
to-Segura P Abstract The NF-kappaB pathway might play a role in the pathogenesis of renal disease and type 2 diabetes (T2DM). Our aim was to determine whether common polymorphisms in NF-kappaB genes were associated with impaired renal function and T2DM in a cohort of healthy elderly individuals. We studied 487 individuals, all Caucasian and aged 65-85 years. A total of 104 (21%) had impaired renal function (estimated glomerular filtration rate, eGFR
Source: Human Immunology - March 27, 2018 Category: Allergy & Immunology Authors: Coto E, Corte CD, Tranche S, Gómez J, Alonso B, Iglesias S, Reguero JR, López-Larrea C, Coto-Segura P Tags: Hum Immunol Source Type: research

HLA-G is expressed in intestinal samples of ulcerative colitis and Crohn's disease patients and HLA-G5 expression is differentially correlated with TNF and IL-10 cytokine expression.
Abstract HLA-G is an immunomodulatory molecule that can be produced by epithelial cells. Considering that TNF and IL-10 participate in bowel inflammatory disorders and that both cytokines modulate HLA-G, we evaluated HLA-G, TNF and IL-10 mRNA expression by qPCR and HLA-G protein levels by immunohistochemistry in two intestinal samples exhibiting different degree of inflammation within a patient suffering from Crohn's disease (CD) or ulcerative colitis (UC). Tissue HLA-G5 (P
Source: Human Immunology - March 24, 2018 Category: Allergy & Immunology Authors: Gomes RG, de Brito CAA, Martinelli VF, Dos Santos RN, Dos Santos Gomes FO, Peixoto CA, Crispim JO, Diniz GTN, Donadi EA, Lucena-Silva N Tags: Hum Immunol Source Type: research

Characterizing Alleles with Large Deletions Using Region Specific Extraction.
Abstract Two novel HLA class II alleles, DRB4∗03:01N and DQB1∗03:276N, containing large deletions were identified during routine typing. Extraction of DNA encompassing the deletions was carried out with a panel of capture oligonucleotides followed by whole genome amplification. Next generation DNA sequencing was then used to characterize the sequences. DRB4∗03:01N has a 16 kilobase pair deletion stretching upstream from intron 2 toward centromeric DRB8. DQB1∗03:276N has two deletions separated by 844 nucleotides. The first deletion (3.7 kilobase pairs) is upstream of intron 1 and the se...
Source: Human Immunology - March 23, 2018 Category: Allergy & Immunology Authors: Steiner NK, Hou L, Hurley CK Tags: Hum Immunol Source Type: research

Correlations Between Donor-Specific Antibodies and Non-Adherence with Chronic Active Antibody-Mediated Rejection Phenotypes and Their Impact On Kidney Graft Survival.
ita A Abstract Chronic-active antibody-mediated rejection (CAABMR) is associated with poor kidney graft survival and has no clear effective treatment. Forty-one cases of CAABMR were detected in indication graft biopsies and evaluated according to current Banff classification. We investigated the impact of concurrent donor-specific antibodies (DSA) and their characteristics, together with non-adherence regarding immunosuppression on CAABMR histopathological phenotypes and prognosis. Twenty-four (59%) patients had detectable DSA at biopsy, with 15 of them being considered non-adherent. Graft function at biopsy was s...
Source: Human Immunology - March 22, 2018 Category: Allergy & Immunology Authors: Malheiro J, Santos S, Tafulo S, Dias L, Salete Martins, Fonseca I, Almeida M, Pedroso S, Beirão I, Castro-Henriques A, Cabrita A Tags: Hum Immunol Source Type: research

HLA-G and vertical mother-to-child transmission of human papillomavirus infection.
l;nen S Abstract Role of host factors in transmission of human papillomavirus (HPV)-infection from mother to her offspring is not known. Our aim was to study whether human leukocyte antigen (HLA)-G allele concordance among the mother-child pairs could facilitate vertical transmission of HPV, because HLA-G may contribute to immune tolerance in pregnancy. Altogether, 310 mother-child pairs were included from the Finnish Family HPV study. Overall, nine different HLA-G alleles were identified. The HLA-G genotype concordance of G∗01:01:01/01:04:01 increased the risk of high risk (HR)-HPV genotype positivity in c...
Source: Human Immunology - March 12, 2018 Category: Allergy & Immunology Authors: Louvanto K, Roger M, Faucher MC, Syrjänen K, Grenman S, Syrjänen S Tags: Hum Immunol Source Type: research

CD8+CXCR5+ T cells in tumor-draining lymph nodes are highly activated and predict better prognosis in colorectal cancer.
Abstract Tumor-draining lymph nodes (TDLNs) are the primary sites of tumor antigen presentation, as well as the origin of metastasis in most cases. Hence, the type and function of immune cells in TDLNs are critical to the microenvironment and potentially affect the clinical outcome of the malignancy. CD8+CXCR5+ T cells are recently described to present high effector functions in infectious diseases, but their role in colorectal cancer (CRC) remains unclear. In forty-four Stage III CRC patients, we examined the CD8+CXCR5+ T cells in blood, tumor, and TDLN. CD8+CXCR5+ T cells represented lass than 2% of CD3+ T cells...
Source: Human Immunology - March 12, 2018 Category: Allergy & Immunology Authors: E J, Yan F, Kang Z, Zhu L, Xing J, Yu E Tags: Hum Immunol Source Type: research

Impact of persistent and cleared preformed HLA DSA on kidney transplant outcomes.
P, Marta C Abstract Preformed HLA donor-specific antibodies (DSA) only detected with Luminex have been associated with increased risk of antibody-mediated rejection (ABMR) and graft failure after kidney transplantation (KT). Their evolution after KT may modify this risk. We analyzed postransplant evolution of preformed DSA identified retrospectively and their impact on outcomes of 370 KT performed 2006-2014. Antibodies were monitored prospectively at 1-3-5 years after KT and if any dysfunction. Early acute ABMR was more frequent among patients with preformed DSA class-I or I+II than isolated class-II (29.4% vs 4....
Source: Human Immunology - March 7, 2018 Category: Allergy & Immunology Authors: Dolores RP, José PM, Marisa M, Javier G, Laura L, Carmen G, Juan José H, Jose Y, Julio P, Marta C Tags: Hum Immunol Source Type: research

Vitamin D3 inhibits the proliferation of T helper cells, downregulate CD4+ T cell cytokines and upregulate inhibitory markers.
Abstract 1 α, 25-dihydroxyvitamin D3 (VitD3) has been suggested to have strong modulatory properties in the immune system. Researchers in the present study primarily aimed to understand the effect of VitD3 on human CD4+ T cell proliferation in antigen presenting cells (APCs) free condition in vitro. The effect of VitD3 on intracellular cytokine responses trend to Th1, Th2, Th17 and Th22 was evaluated using the flow cytometry. Moreover the effect of VitD3 on the expression of inhibitory markers such as PD1, PD-L1, and CTLA4 which are induced upon polyclonal T cell receptor (TCR) activation on CD4+ T cells, wa...
Source: Human Immunology - March 6, 2018 Category: Allergy & Immunology Authors: Sheikh V, Kasapoglu P, Zamani A, Basiri Z, Tahamoli-Roudsari A, Alahgholi-Hajibehzad M Tags: Hum Immunol Source Type: research

Clinicopathologic significance of HLA-G and HLA-E molecules in Tunisian patients with ovarian carcinoma.
CONCLUSION: HLA-G and HLA-E are highly represented in ovarian carcinoma suggesting a potential association with progressive disease mechanism. HLA-G and HLA-E molecules might be new candidates' markers for ovarian carcinoma progression. PMID: 29499226 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - February 27, 2018 Category: Allergy & Immunology Authors: Babay W, Yahia HB, Boujelbene N, Zidi N, Laaribi AB, Kacem D, Ghorbel RB, Boudabous A, Ouzari HI, Rizzo R, Rebmann V, Mrad K, Zidi I Tags: Hum Immunol Source Type: research

Clinical Transplant Tolerance: Coming of Age.
PMID: 29496488 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - February 26, 2018 Category: Allergy & Immunology Authors: Mathew JM, Leventhal JR Tags: Hum Immunol Source Type: research

Transcriptomic studies in Tolerance: Lessons learned and the path forward.
The objective of this article is to first, discuss the progress made on genomic biomarkers of immunological tolerance and the future avenues for the development of such biomarkers specifically in kidney transplantation. Secondly, we provide a set of guiding principles and identify the pitfalls, advantages, and drawbacks of studies that generate genomic data aimed at understanding transplant tolerance that is applicable to all solid transplants. PMID: 29481826 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - February 23, 2018 Category: Allergy & Immunology Authors: Kurian SM, Whisenant TC, Mathew JM, Miller J, Leventhal JR Tags: Hum Immunol Source Type: research

Biomarkers of immune tolerance in liver transplantation.
eyo A Abstract The liver exhibits intrinsic immune tolerogenic properties that contribute to a unique propensity toward spontaneous acceptance when transplanted, both in animal models and in humans. Thus, in contrast to what happens after transplantation of other solid organs, several years following liver transplantation a significant subset of patients are capable of maintaining normal allograft function with histological integrity in the absence of immunosuppressive drug treatment. Significant efforts have been put into identifying sensitive and specific biomarkers of tolerance in order to stratify liver transp...
Source: Human Immunology - February 17, 2018 Category: Allergy & Immunology Authors: Vionnet J, Sánchez-Fueyo A Tags: Hum Immunol Source Type: research

B cells in operational tolerance.
Abstract Transplantation is currently the therapy of choice for endstage organ failure even though it requires long-term immunossuppresive therapy, with its numerous side effects, for acceptance of the transplanted organ. In rare cases however, patients develop operational tolerance, that is, graft survival without immunosuppression. Studies conducted on these patients reveal genetic, phenotypic, and functional signatures. They provide a better understanding of the immunological mechanisms involved in operational tolerance and define biomarkers that could be used to adapt immunosuppressive treatment to the individ...
Source: Human Immunology - February 16, 2018 Category: Allergy & Immunology Authors: Chesneau M, Danger R, Soulillou JP, Brouard S Tags: Hum Immunol Source Type: research

IL12B, IL23A, IL23R and HLA-C ∗06 genetic variants in psoriasis susceptibility and response to treatment.
IL12B, IL23A, IL23R and HLA-C∗06 genetic variants in psoriasis susceptibility and response to treatment. Hum Immunol. 2018 Feb 15;: Authors: Bojko A, Ostasz R, Białecka M, Klimowicz A, Malinowski D, Budawski R, Bojko P, Droździk M, Kurzawski M Abstract The role of interleukin-23 is crucial in the pathogenesis of psoriasis, and IL23A, IL12B and IL23R genetic variants have been associated with the disease in genome-wide association studies. In the current paper we have conducted a confirmation study of the abovementioned genetic factors in a case-control analysis of 507 psoriatic patients and 3...
Source: Human Immunology - February 15, 2018 Category: Allergy & Immunology Authors: Bojko A, Ostasz R, Białecka M, Klimowicz A, Malinowski D, Budawski R, Bojko P, Droździk M, Kurzawski M Tags: Hum Immunol Source Type: research

Association of polymorphic variants of IL-1 β and IL-1RN genes in the development of Graves' disease in Kashmiri population (North India).
CONCLUSION: Our findings suggest that both promoter and exon polymorphisms of IL-1β gene have a significant role in the risk of developing GD, whereas IL-1RNVNTR has no association with GD. PMID: 29454070 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - February 14, 2018 Category: Allergy & Immunology Authors: Shehjar F, Afroze D, Misgar RA, Malik SA, Laway BA Tags: Hum Immunol Source Type: research