Utilizing nanopore sequencing technology for the rapid and comprehensive characterization of eleven HLA loci; addressing the need for deceased donor expedited HLA typing.
This report demonstrates the benefits of nanopore sequencing introduced by Oxford Nanopore Technologies (ONT) for HLA genotyping. Samples (n = 120) previously characterized at high-resolution three-field (HR-3F) for 11 loci were assessed using ONT sequencing paired to a single-plex PCR protocol (Holotype) and to two multiplex protocols OmniType (Omixon) and NGSgo®-MX6-1 (GenDx). The results demonstrate the potential of nanopore sequencing for delivering accurate HR-3F typing with a simple, rapid, and cost-effective protocol. The protocol is applicable to time-sensitive applications, such as deceased donor typ...
Source: Human Immunology - June 25, 2020 Category: Allergy & Immunology Authors: Mosbruger TL, Dinou A, Duke JL, Ferriola D, Mehler H, Pagkrati I, Damianos G, Mbunwe E, Sarmady M, Lyratzakis I, Tishkoff SA, Dinh A, Monos DS Tags: Hum Immunol Source Type: research

HLA-A, -B, and -DRB1 genotyping and haplotype frequencies among Filipinos living in the National Capital Region of the Philippines.
In this report, HLA polymorphisms (A, B, and DRB1 loci) were determined in 51 unrelated Filipinos. Molecular genotyping was carried out by sequence-based typing (Sanger sequencing). Data were analyzed by HLA-net GENE[RATE] tools. HLA-A, -B and -DRB1 genotype frequencies were at Hardy-Weinberg equilibrium. A*11, B*15, and DRB1*15 were the most frequent allele groups, while A*11-B*15-DRB1*15 was the most frequent HLA-A-B-DRB1 haplotype. HLA data are available in the Allele Frequencies Net Database (AFND: 3690) under the population name "Philippines National Capital Region". PMID: 32571529 [PubMed - as supplied...
Source: Human Immunology - June 19, 2020 Category: Allergy & Immunology Authors: Villanueva EQ, Li CC, Lin PY, Yang KL Tags: Hum Immunol Source Type: research

Concordance between predicted HLA type using next generation sequencing data generated for non-HLA purposes and clinical HLA type.
Abstract We explored the feasibility of obtaining accurate HLA type using pre-existing NGS data not generated for HLA purposes. 83 exomes and 500 targeted NGS pharmacogenomic panels were analyzed using Omixon HLA Explore, OptiType, and/or HLA-Genotyper software. Results were compared against clinical HLA genotyping. 765 (94.2%) Omixon and 769 (94.7%) HLA-Genotyper of 812 germline allele calls across class I/II loci and 402 (99.5%) of 404 OptiType class I calls were concordant to the second field (i.e. HLA-A*02:01). An additional 19 (2.3%) Omixon, 39 (4.8%) HLA-Genotyper, and 2 (0.5%) OptiType allele calls were fir...
Source: Human Immunology - June 13, 2020 Category: Allergy & Immunology Authors: Moyer AM, Dukek B, Duellman P, Schneider B, Wakefield L, Skierka JM, Avula R, Bhagwate AV, Kalari KR, Kreuter JD, Goetz MP, Boughey JC, Black JL, Gandhi MJ Tags: Hum Immunol Source Type: research

Genetics of Mexico Jamiltepec Oaxaca Mixtec Amerindians according to HLA genes.
Abstract Mexican Mixtec population from coastal Jamiltepec (Oaxaca) Amerindians was studied for its HLA profile. They show genetic characteristics close to Pacific Islanders and other Mexican Isthmus Amerindians (Mazatecans, Zapotecans and Mayas). Interestingly, this coastal Oaxaca Mixtec population is genetically closer to Mazatecans than to Oaxaca Mixtec from mountains according to HLA genes. Mixtec HLA frequent extended haplotype A*24:02-B*35:14-DRB1*16:02 has been also found in Jaidukama North Colombia forest Amerindians and in Guatemala Mayas; A*24:02, DRB1*04:03, DRB1*04:04 and DRB2*16:02 are frequent allele...
Source: Human Immunology - June 11, 2020 Category: Allergy & Immunology Authors: Arnaiz-Villena A, Suarez-Trujillo F, Lopez-Nares A, Crespo-Yuste E, Callado A, Juarez I Tags: Hum Immunol Source Type: research

T follicular helper cell-mediated IL-21 production suppresses FOXP3 expression of T follicular regulatory-like cells in diffuse large B cell lymphoma patients.
In this study, we investigated the transcription factors and regulatory elements associated with Tfr-like cells in DLBCL patients. Both circulating and tumor-infiltrating Tfr-like cells presented slightly higher Blimp-1 expression and significantly higher Foxp3 expression than the CD25- Tfh subset. As the IL-2 receptor, CD25 could be moderately upregulated in stimulated CD25- Tfh cells. However, stimulated CD25- Tfh cells could not upregulate Foxp3, indicating that the distinction between Foxp3-low CD25-CXCR5+CD4+ T cells and Foxp3-high CD25+CXCR5+CD4+ T cells was not due to differences in stimulation status. Regarding cyt...
Source: Human Immunology - June 10, 2020 Category: Allergy & Immunology Authors: Ma X, Zha J, He J, Chen L, Huang J, Wu W, Tian P, Qian BH, Yu L, Jiang Y, Xu B Tags: Hum Immunol Source Type: research

Corrigendum to "A new set of reagents and related software used for NGS based classical and non-classical HLA typing showing evidence for a greater HLA haplotype diversity" [Hum. Immunol. 81 (2020) 202-205].
Corrigendum to "A new set of reagents and related software used for NGS based classical and non-classical HLA typing showing evidence for a greater HLA haplotype diversity" [Hum. Immunol. 81 (2020) 202-205]. Hum Immunol. 2020 Jun 07;: Authors: Alizadeh M, Picard C, Frassati C, Walencik A, Gauthier AC, Bennasar F, Verite F, Semana G PMID: 32522359 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - June 7, 2020 Category: Allergy & Immunology Authors: Alizadeh M, Picard C, Frassati C, Walencik A, Gauthier AC, Bennasar F, Verite F, Semana G Tags: Hum Immunol Source Type: research

Clinical utility of next generation sequencing based HLA typing for disease association and pharmacogenetic testing.
r-Molnár E Abstract HLA associations have been linked to many diseases and are important for risk assessment of drug hypersensitivity reactions. The increasing number of HLA alleles discovered generated a list of ambiguities that cannot be resolved with the current clinical assays, which commonly include sequence-specific oligonucleotide probe (SSOP) genotyping, and real-time PCR with melting curve analysis. HLA typing by next-generation sequencing (NGS) has recently been adopted by clinical laboratories for transplantation testing, as it provides unambiguous and cost-effective HLA typing. The goal of this ...
Source: Human Immunology - June 1, 2020 Category: Allergy & Immunology Authors: Profaizer T, Pole A, Monds C, Delgado JC, Lázár-Molnár E Tags: Hum Immunol Source Type: research

Distribution of CD36 deficiency in different Chinese ethnic groups.
CONCLUSIONS: Through an analysis of a large cohort, we determined the frequencies of CD36 deficiency among different Chinese ethnic groups. A high frequency of type-I deficiency was found in certain minorities living in southern China, which is known to be vulnerable to malaria epidemics. These findings may help us understand the phenotypic consequences of CD36-deficient alleles associated with malaria. PMID: 32487483 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - May 30, 2020 Category: Allergy & Immunology Authors: Liu J, Shao Y, Ding H, Deng J, Xu X, Wang J, Xia W, Santoso S, Ye X, Fu Y Tags: Hum Immunol Source Type: research

Non-donor specific anti-human leukocyte antigen (HLA) antibodies are not associated with poor outcome in hematopoietic stem cell transplant recipients.
We present the results of a retrospective cohort analysis of 157 patients who received HSCT at the University of Rochester over a period of four years. We identified 45 patients (28.7%) who had detectable anti-HLA antibodies, while only one patient (0.6%) had donor-specific anti-HLA antibodies. Patients with prior pregnancies and multiple transfusions were at increased risk to develop antibodies. In our cohort, the presence of non-donor specific anti-HLA antibodies did not significantly impact overall survival, progression free survival, graft failure, or transplant-related mortality. PMID: 32471661 [PubMed - as suppl...
Source: Human Immunology - May 26, 2020 Category: Allergy & Immunology Authors: Andolina JR, Walia R, Oliva J, Baran A, Liesveld J, Becker MW, Busacco A, Coppage M Tags: Hum Immunol Source Type: research

CD1 gene polymorphism and susceptibility to celiac disease: Association of CD1E*02/02 in Moroccans.
Abstract CD1 glycoproteins are a class of antigen presenting molecules that bind and present non-peptidic antigens (lipids and glycolipids) for immune recognition. CD1 polymorphisms, although limited, could have a critical role in antimicrobial, anticancer, and autoimmune responses and disease susceptibility. Ethnic differences and interactions between genetic and environmental factors make it attractive the study of these molecules in autoimmune inflammatory disorders, such as celiac disease (CD), in which a strong genetic predisposition (HLA-DQ2/DQ8) and pressure of environmental factors have a central role. CD1...
Source: Human Immunology - May 25, 2020 Category: Allergy & Immunology Authors: Aureli A, Aboulaghras S, Oumhani K, Del Beato T, Sebastiani P, Colanardi A, El Aouad R, Ben El Barhdadi I, Piancatelli D Tags: Hum Immunol Source Type: research

HLA study in Bolivian Quechua Amerindians from Titikaka Lake Area.
Abstract Quechua Amerindians established Inca Empire and chose Cuzco as their capital. Their language is closely related to that of Aymara ethnic group and both of them were originated from Titikaka Lake Altiplano area. In the present study we have analyzed Bolivian Quechua HLA profile and found that it has common characters with other Andean and Pacific Amerindians (Uros, Aymaras, Lamas, Mapuches, Athabascan), and Pacific Islanders, including Easter Islanders: relatively high frequency of HLA-A*24 (:02), class II haplotypes DRB1*08:02-DQB1*04:02, and DRB1*04:03-DQB1* 03:02. Titikaka Lake area prehistoric populati...
Source: Human Immunology - May 14, 2020 Category: Allergy & Immunology Authors: Arnaiz-Villena A, Juarez I, Lopez-Nares A, Crespo-Yuste E, Callado A, Suarez-Trujillo F Tags: Hum Immunol Source Type: research

A long road/read to rapid high-resolution HLA typing: The nanopore perspective.
Abstract Next-generation sequencing (NGS) has been widely adopted for clinical HLA typing and advanced immunogenetics researches. Current methodologies still face challenges in resolving cis-trans ambiguity involving distant variant positions, and the turnaround time is affected by testing volume and batching. Nanopore sequencing may become a promising addition to the existing options for HLA typing. The technology delivered by the MinION sequencer of Oxford Nanopore Technologies (ONT) can record the ionic current changes during the translocation of DNA/RNA strands through transmembrane pores and translate the sig...
Source: Human Immunology - May 1, 2020 Category: Allergy & Immunology Authors: Liu C Tags: Hum Immunol Source Type: research

Distribution of HLA-A, HLA-B, HLA-C, and HLA-DRB1 alleles and haplotypes in Jingpo minority in Yunnan province of China.
Abstract HLA-A, HLA-B, HLA-C, and HLA-DRB1 allele frequencies and estimated haplotype frequencies from 105 unrelated healthy Jingpo ethnic subjects who living in Dehong Dai-Jingpo Autonomous Prefectures, Yunnan Province, southwest China has been reported. HLA genes were genotyped by SSOP typing method using Luminex Multi-Analyte Profiling system. Jingpo ethnic nationality belongs to southern group of East Asians, but with its specific HLA alleles and haplotypes characteristic. PMID: 32359785 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 29, 2020 Category: Allergy & Immunology Authors: Tao Y, Shi L, Liu S, Yao Y, Shi L Tags: Hum Immunol Source Type: research

Detecting mismatched donor HLA types from allograft biopsies - An easily applicable tool for improved individualized risk assessment.
sen P Abstract Short-term allograft survival has improved among solid organ transplant (SOT) patients. An increasing number of SOT patients are prepared for re-transplantation because of chronic allograft failure. Lack of HLA typing or incomplete HLA typing of previous donors complicates pretransplant risk assessment, as repeated HLA mismatches may be missed. In addition, a complete HLA type of the donor is essential in the diagnosis of antibody-mediated rejection. We aimed to determine donor HLA types from allograft biopsies from kidney, heart and liver grafts. Graft biopsies were obtained from 13 kidney, heart a...
Source: Human Immunology - April 28, 2020 Category: Allergy & Immunology Authors: Berg R, Nørgaard M, Bruun MT, Christiansen M, Koefoed-Nielsen P Tags: Hum Immunol Source Type: research

Novel immunological approach to asses donor reactivity of transplant recipients using a humanized mouse model.
Abstract In organ transplantation, a reproducible and robust immune-monitoring assay has not been established to determine individually tailored immunosuppressants (IS). We applied humanized mice reconstituted with human (hu-) peripheral blood mononuclear cells (PBMCs) obtained from living donor liver transplant recipients to evaluate their immune status. Engraftment of 2.5 × 106 hu-PBMCs from healthy volunteers and recipients in the NSG mice was achieved successfully. The reconstituted lymphocytes consisted mainly of hu-CD3+ lymphocytes with predominant CD45RA-CD62Llo TEM and CCR6-CXCR3+CD4+ Th1 c...
Source: Human Immunology - April 25, 2020 Category: Allergy & Immunology Authors: Fukasaku Y, Goto R, Ganchiku Y, Emoto S, Zaitsu M, Watanabe M, Kawamura N, Fukai M, Shimamura T, Taketomi A Tags: Hum Immunol Source Type: research

Development of de novo HLA donor specific antibodies (HLA-DSA), HLA antibodies (HLA-Ab) and allograft rejection post blood transfusion in kidney transplant recipients.
CONCLUSION: Blood transfusion under strong immunosuppressive cover within a one-week post-operative period is safe with no significant association with the development of de novo HLA-DSA, HLA-Ab or clinical rejection. PMID: 32327243 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 20, 2020 Category: Allergy & Immunology Authors: Jalalonmuhali M, Carroll RP, Tsiopelas E, Clayton P, Coates PT Tags: Hum Immunol Source Type: research

HLA study in Amerindian Bolivia La Paz Aymaras.
Abstract Aymara people has been a relatively homogeneous group since Spanish Conquest by 1,532 CE, even if previously represented a group of various cultural defined populations who gave rise to them. They were and are established in Andean Altiplano around Titikaka Lake (Bolivia, Peru), Argentina and Chile neighborhood, speak Aymara language and have been maintained after Europeans arrival at a lower social status than Quechua (Inca) speaking people. However, both Aymara and Quechua populations acknowledge Titikaka Lake as center of their origins; both languages are also related. Specific high frequencies of HLA-...
Source: Human Immunology - April 20, 2020 Category: Allergy & Immunology Authors: Arnaiz-Villena A, Juarez I, Lopez-Nares A, Crespo-Yuste E, Callado A, Suarez-Trujillo F Tags: Hum Immunol Source Type: research

HLA-A, -B, -C, -DRB1 and -DQB1 alleles and haplotypes in 271 Southeast Asia Indians from Peninsular Malaysia.
In this report, HLA-B and HLA-DQB1 was in Hardy-Weinberg proportions (HWEP) (p > 0.05). We observed significant deviation from the HWEP for HLA-A (p 
Source: Human Immunology - April 17, 2020 Category: Allergy & Immunology Authors: Nurul-Aain AF, Tan LK, Heselynn H, Nor-Shuhaila S, Eashwary M, Wahinuddin S, Lau IS, Gun SC, Mohd-Shahrir MS, Ainon MM, Azmillah R, Muhaini O, Shahnaz M, Too CL Tags: Hum Immunol Source Type: research

Naming HLA diversity: A review of HLA nomenclature.
Abstract The development of a standardized HLA nomenclature has been critical in our understanding of the HLA system and in facilitating the clinical applications of HLA. The Nomenclature Committee for Factors of the HLA System, established in 1968, has overseen the development and usage of nomenclature based on serologic specificities, cellular responses, and DNA sequences. Their decisions have been guided by community consensus reached through 17 international workshops beginning in 1964 and continuing today. Two websites provide a curated database of the sequences of over 26,000 HLA alleles and a reference site...
Source: Human Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Hurley CK Tags: Hum Immunol Source Type: research

HLA-DQ mismatches stimulate de novo donor specific antibodies in heart transplant recipients.
Abstract The development of donor specific antibody is associated with graft rejection and increased mortality in solid organ transplant recipients. The majority of de novo donor specific antibodies (dnDSA) are against HLA-DQ antigens, but it has not been investigated if this is caused by more mismatches in the HLA-DQ locus between the recipient and donor. Here we examined the impact of HLA mismatches in eight HLA loci on the development of dnDSA and on rejection in a large cohort of heart transplant recipients. We evaluated HLA mismatches at the antigen level, the eplet level using HLAMatchmaker, and the epitope ...
Source: Human Immunology - April 16, 2020 Category: Allergy & Immunology Authors: Zhang X, Kransdorf E, Levine R, Patel JK, Kobashigawa JA Tags: Hum Immunol Source Type: research

Next generation sequencing of 11 HLA loci characterises a diverse UK cord blood bank.
In this study, DNA samples from 714 National Health Service (NHS) Cord Blood Bank donors were typed for HLA-A, -B, -C, -DRB1, -DRB345, -DQA1, -DQB1, -DPA1 and -DPB1 by NGS. Analysis of the ethnic diversity showed a high level of diversity, with the cohort comprising of 62.3% European and 37.7% of either multi-ethnic or non-European donors, of which 12.3% were multi-ethnic. The HLA diversity was further confirmed using PyPop analysis, 405 distinct alleles were observed in the overall NHS-CBB cohort, of which 37 alleles are non-CWD, including A*31:14N, B*35:68:02, C*14:23 and DQA1*05:10. Furthermore, HLA-DQA1 and HLA-DPA1 an...
Source: Human Immunology - April 15, 2020 Category: Allergy & Immunology Authors: Guerra SG, Hamilton-Jones S, Brown CJ, Navarrete CV, Chong W Tags: Hum Immunol Source Type: research

Luminex screening first vs. direct single antigen bead assays: Different strategies for HLA antibody monitoring after kidney transplantation.
CONCLUSIONS: Luminex screening failed to identify clinically relevant HLA antibodies, hampering DSA detection in patients with possible ABMR. Direct SAB testing should be the chosen strategy for post-transplantation monitoring, albeit direct SAB from the two existing manufacturers may diverge in as much as 50% of cases. PMID: 32279925 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - April 9, 2020 Category: Allergy & Immunology Authors: Burballa C, Pérez-Saéz MJ, Redondo-Pachón D, García C, Mir M, Arias-Cabrales C, Valenzuela NM, Reed EF, Pascual J, Crespo M Tags: Hum Immunol Source Type: research

Overrepresentation of highly functional T regulatory cells in patients with nonfunctioning pituitary adenoma.
Abstract Nonfunctioning pituitary adenoma is a common intracranial tumor. Though benign in the majority of cases, complications can be excruciating to the affected individual, and recurrences after tumor removal may happen with more aggressive clinical features. T regulatory (Treg) cells are generally considered a tumor-promoting immune cell type in malignant cancers with currently unclear roles in pituitary adenoma patients. Therefore, we investigated the frequency and functional characteristics of Treg cells in nonfunctioning pituitary adenoma patients before and after tumor removal. Compared to healthy controls...
Source: Human Immunology - April 9, 2020 Category: Allergy & Immunology Authors: Han S, Ma E, Jiang W, Lu Y, Sun X, Feng S Tags: Hum Immunol Source Type: research

Expression analysis of immune-regulatory molecules HLA-G, HLA-E and IDO in endometrial cancer.
Abstract HLA-G has been widely implicated in advanced cancers through different pathways of immunosuppression allowing tumor escape. Contrarily, HLA-E has a controversial role in the tumor escape from the immune system. IDO catabolic enzyme is known to be up-regulated in many tumors types allowing their immune escape. Based on these considerations, we investigated the expression of HLA-G, HLA-E and IDO molecules in endometrial cancer (EC) and their association with prognostic clinicopathologic parameters. Their expression were checked in tumoral and adjacent endometrial tissues. Both HLA-G and IDO immunostaining w...
Source: Human Immunology - April 6, 2020 Category: Allergy & Immunology Authors: Ben Yahia H, Boujelbene N, Babay W, Ben Safta I, Dhouioui S, Zemni I, Ali Ayadi M, Charfi L, Ouzari HI, Rebmann V, Rizzo R, Mrad K, Driss M, Zidi I Tags: Hum Immunol Source Type: research

HLA-A, -B, -DRB1 and -DQB1 polymorphisms among Iraqi Arabs.
In this report, HLA polymorphisms (A, B, DRB1 and DQB1 loci) were determined in 149 unrelated Iraqi Arab potential bone marrow and kidney donors. Molecular genotyping was carried out by polymerase chain reaction followed by specific oligonucleotide probe hybridizations. Data were analyzed by Arlequin software. HLA-A, -B and -DRB1 genotype frequencies were significantly deviated from Hardy-Weinberg equilibrium, while HLA-DQB1 frequencies showed no deviation. A*03, B*35, DRB1*11 and DQB1*02 were the most frequent allele groups, while A*02-B*07-DRB1*04-DQB1*03 was the most frequent haplotype. HLA data are available in the All...
Source: Human Immunology - March 26, 2020 Category: Allergy & Immunology Authors: Ad'hiah AH, Al-Rikabi AH, Ahmed ZA, Kamil LA Tags: Hum Immunol Source Type: research

HLA genes in Amerindians from Mexico San Vicente Tancuayalab Teenek/Huastecos.
Abstract Huastecos or Teenek Amerindians are presently living at North East Mexico (San Luis Potosi State). They have probably one of the most ancient culture of Mexico and Central America together with Mayas and Olmec groups with which also show close relationships. Proximity to Atlantic Ocean/Mexican Gulf originated that Spaniards had very early contact with them at about 1519 CE or before. In the present paper we have aimed to study HLA gene profile which may be useful for HLA and disease epidemiology and transplant programs in Teeneks. HLA-DRB1*04:07, -DRB1*14:06 and -DRB1*04:11 have been found in high fr...
Source: Human Immunology - March 21, 2020 Category: Allergy & Immunology Authors: Arnaiz-Villena A, Juarez I, Suarez-Trujillo F, Crespo-Yuste E, Lopez-Nares A, Callado A, Vaquero C, Vargas-Alarcon G Tags: Hum Immunol Source Type: research

Impact of donor KIRs and recipient KIR/HLA class I combinations on GVHD in patients with acute leukemia after HLA-matched sibling HSCT.
Abstract In addition to T cells, NK cells can also participate in the outcome of hematopoietic stem cell transplantation (HSCT) mainly through the interaction between donor killer cell immunoglobulin-like receptors (KIRs) and recipient human leukocyte antigen (HLA) class I molecules. There is a risk of GVHD other than leukemia relapse after allogeneic HSCT that activation of donor NK cells in the absence of appropriate inhibitory ligands will be one of the reasons. To investigate the impact of donor KIRs and recipient KIR/HLA class I combinations on GVHD and leukemia relapse in patients with acute leukemia after H...
Source: Human Immunology - March 18, 2020 Category: Allergy & Immunology Authors: Mansouri M, Villard J, Ramzi M, Alavianmehr A, Farjadian S Tags: Hum Immunol Source Type: research

NGS and HLA: The long road ahead.
Abstract This manuscript is a continuation of this laboratory's journey to identifying novel HLA alleles while performing routine clinical HLA laboratory testing. Since our last paper, we have identified an additional 28 novel HLA alleles that are identified and described herein. One novel allele was found in two unrelated patients that were HLA typed for different reasons at two different times, suggesting that novel alleles may be much more frequent than previously expected. If the rate of identification is hindered by bioinformatics challenges, there is a great potential for our patients to suffer needlessly fr...
Source: Human Immunology - March 16, 2020 Category: Allergy & Immunology Authors: Ingram KJ, O'Shields EF, Kiger DF, Gautreaux MD Tags: Hum Immunol Source Type: research

New genetic predictors for abacavir tolerance in HLA-B*57:01 positive individuals.
Abstract Abacavir hypersensitivity syndrome (ABC HSS) is strongly associated with carriage of human leukocyte antigen (HLA)-B*57:01, which has a 100% negative predictive value for the development of ABC HSS. However, 45% of individuals who carry HLA-B*57:01 can tolerate ABC. We investigated immune and non-immune related genes in ABC HSS (n = 95) and ABC tolerant (n = 43) HLA-B*57:01 + patients to determine other factors required for the development of ABC HSS. Assignment of phenotype showed that ABC HSS subjects were significantly less likely than tolerants to carry only ERAP1 hypoact...
Source: Human Immunology - March 12, 2020 Category: Allergy & Immunology Authors: Pavlos R, Deshpande P, Chopra A, Leary S, Strautins K, Nolan D, Thorborn D, Shaefer M, Rauch A, Dunn D, Montaner J, Rachlis A, Almeida CA, Choo L, James I, Redwood AJ, Li Y, Gaudieri S, Mallal SA, Phillips EJ Tags: Hum Immunol Source Type: research

Study of HLA genes in Mexico Mayo/Yoremes Amerindians: Further support of gene exchange with Pacific Islanders.
This study also supports that Americas peopling was not only carried out through Bering Strait but also through Pacific and Atlantic Oceans in an earlier time than proposed. PMID: 32164964 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - March 9, 2020 Category: Allergy & Immunology Authors: Arnaiz-Villena A, Juarez I, Crespo-Yuste E, Lopez-Nares A, Callado A, Vargas-Alarcon G, Vaquero C, Suarez-Trujillo F Tags: Hum Immunol Source Type: research

The pro-Inflammatory cytokines effects on mobilization, self-renewal and differentiation of hematopoietic stem cells.
Abstract Different mature immune cells are being produced during infection by hematopoietic stem cells in order to compensate the required cells battling against pathogens. A wide variety of pro-inflammatory cytokines like G-CSF, TNFα, IFNα, IL-1, IL-6, etc. affect HSCs and provoke different responses including maintenance, survival, activation, proliferation, differentiation, and immune responses. The exact mechanisms of translating pathogen sensing into cytokine signals for regulating HSCs are not fully understood yet. Moreover, the interaction between these cytokines modulating different responses h...
Source: Human Immunology - March 2, 2020 Category: Allergy & Immunology Authors: Jahandideh B, Derakhshani M, Abbaszadeh H, Akbar Movassaghpour A, Mehdizadeh A, Talebi M, Yousefi M Tags: Hum Immunol Source Type: research

THE action of TH17 cells on blood brain barrier in multiple sclerosis and experimental autoimmune encephalomyelitis.
In conclusion, Th17 cells can efficiently cross the BBB using pathways distinct from those used by Th1 cells, leading to BBB disruption, the activation of other inflammatory cells and neurodegeneration in MS patients. PMID: 32122685 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - February 28, 2020 Category: Allergy & Immunology Authors: Balasa R, Barcutean L, Balasa A, Motataianu A, Roman-Filip C, Manu D Tags: Hum Immunol Source Type: research

A new set of reagents and related software used for NGS based classical and non-classical HLA typing showing evidence for a greater HLA haplotype diversity.
Abstract To evaluate the HLA typing performance of a new Long-Range PCR NGS set of reagents and its dedicated software, a panel of 41 reference homozygous cell lines from the International Histocompatibility Working Group (IHWG) and a panel of 376 volunteer bone marrow donors were analyzed for classical and non-classical HLA class I and class II genes. All results, except HLA-DPB1, were obtained without any ambiguities at the 3rd field level. Based on the high resolution performance of the reagents, a number of new alleles have been described not only for classical but also for non-classical HLA class I genes, lea...
Source: Human Immunology - February 28, 2020 Category: Allergy & Immunology Authors: Alizadeh M, Picard C, Frassati C, Walencik A, Gauthier AC, Bennasar F, Verite F, Semana G Tags: Hum Immunol Source Type: research

The B7-H4 gene induces immune escape partly via upregulating the PD-1/Stat3 pathway in non-small cell lung cancer.
Abstract Non-small cell lung cancer (NSCLC) is associated with high mortality rates worldwide. The costimulatory molecule, B7-H4, a member of the B7 family, plays an important role in immune regulation, mainly by inhibiting the proliferation of T cells to achieve a negative regulatory T cell immune response. The mechanism of action of B7-H4 in non-small cell lung cancer is unknown at present. Tumor tissues from 71 patients subjected to radical pneumonectomy were examined, along with NSCLC cells and BALB/c mice. Among the 71 NSCLC cases, overall and recurrence-free survival rates were significantly lower in those d...
Source: Human Immunology - February 26, 2020 Category: Allergy & Immunology Authors: Yuan L, Ye J, Fan D Tags: Hum Immunol Source Type: research

Assessment of a poly-epitope candidate vaccine against Hepatitis B, C, and poliovirus in interaction with monocyte-derived dendritic cells: An ex-vivo study.
Abstract Design and application of epitope-based polyvalent vaccines have recently garnered attention as an efficient alternative for conventional vaccines. We previously have reported the in silico design of HHP antigen which encompasses the immune-dominant epitopes of Hepatitis B surface antigen (HBsAg), Hepatitis C core protein (HCVcp) and Poliovirus viral proteins (VPs). It has been shown that the HHP has desirable conformation to expose the epitopes, high antigenicity and other desired physicochemical and immunological properties. To confirm the accuracy of these predictions, the ex-vivo immunogenicity of the...
Source: Human Immunology - February 26, 2020 Category: Allergy & Immunology Authors: Bahrami AA, Bandehpour M, Kazemi B, Bozorgmehr M, Mosaffa N, Chegeni R Tags: Hum Immunol Source Type: research

Circulating IFN- γ producing CD4+ T cells and IL-17A producing CD4+ T cells, HLA-shared epitope and ACPA may characterize the clinical response to therapy in rheumatoid arthritis patients.
This study analyzed the association between peripheral distributions of helper T cell subsets, HLA shared-epitope (SE), anti-cyclic citrullinated peptide antibody (ACPA) and clinical response to therapy in rheumatoid arthritis (RA) patients. Frequencies of IFN-γ-producing CD4+T (Th1) and IL-17A-producing CD4+T (Th17) cells were determined by flow cytometry in 167 patients (114 cases with good-response (GR) and 53 poor-response (PR) based on DAS28). HLA-DRB1 alleles for patients and 150 healthy controls were determined by PCR-SSP. We observed that 65.2% of RA patients were SE+, 63.4%ACPA+, 43.7%SE+ACPA+ and 14.9% were...
Source: Human Immunology - February 24, 2020 Category: Allergy & Immunology Authors: Alvandpur N, Tabatabaei R, Tahamoli-Roudsari A, Basiri Z, Behzad M, Rezaeepoor M, Roshanaei G, Hajilooi M, Solgi G Tags: Hum Immunol Source Type: research

MICA and KLRK1 genes and their impact in cervical intraepithelial neoplasia development in the southern Brazilian population.
o MDG Abstract Cervical carcinoma and cervical intraepithelial neoplasia (CIN) are associated with persistent infection by oncogenic subtypes of HPV (Human Papillomavirus). Factors linked to immunity, genetics and others like oral contraceptive use, sexual behavior, coinfections with other microorganisms and smoking seem to influence the mechanisms that determine regression or progression to CIN and cervical cancer. We investigated the effect of the MHC class I chain-related gene A (MICA) and Killer Cell Lectin Like receptor K1 (KLRK1) genes on cervical cancer and CIN lesions susceptibility in a group of 195 patie...
Source: Human Immunology - February 24, 2020 Category: Allergy & Immunology Authors: von Linsingen R, Pinho de França P, de Carvalho NS, Bicalho MDG Tags: Hum Immunol Source Type: research

HLA-G and humanized mouse models as a novel therapeutic approach in transplantation.
Abstract HLA-G is a nonclassical MHC-Class I molecule whose expression, along the feto-maternal barrier contributes towards tolerance of the semiallogeneic fetus during pregnancy. In light of its inhibitory properties, recent research has established HLA-G involvement in mechanisms responsible for directing allogeneic immune responses towards tolerance during allogeneic situations such as organ transplantation. Here, we critically review the data supporting the tolerogenic role of HLA-G in organ transplantation, the various factors influencing its expression, and the introduction of novel humanized mouse models th...
Source: Human Immunology - February 21, 2020 Category: Allergy & Immunology Authors: Ajith A, Portik-Dobos V, Horuzsko DD, Kapoor R, Mulloy LL, Horuzsko A Tags: Hum Immunol Source Type: research

Implication of IL-17 producing ɑβT and γδT cells in patients with ovarian cancer.
Implication of IL-17 producing ɑβT and γδT cells in patients with ovarian cancer. Hum Immunol. 2020 Feb 21;: Authors: Chen X, Zhang X, Xu R, Shang W, Ming W, Wang F, Wang J Abstract OBJECTIVE: To investigate the expression and cellular source of IL-17A in human ovarian cancer (OC), benign ovarian tumor (BOT) and borderline ovarian tumor (OBT). METHODS: RT-PCR and immunohistochemistry were used to measure the expression level of IL-17A in human OC tissues. Find concrete source of the elevated IL-17A levels in OC tissues by flow cytometry. RESULTS: We found that IL-17A is exp...
Source: Human Immunology - February 21, 2020 Category: Allergy & Immunology Authors: Chen X, Zhang X, Xu R, Shang W, Ming W, Wang F, Wang J Tags: Hum Immunol Source Type: research

Cytometry-based analysis of HLA-G functions according to ILT2 expression.
Abstract HLA-G was described as a molecule inhibiting NK and T cells functions through its receptor, ILT2. However, most functional studies of HLA-G were so far performed on heterogeneous immune populations and regardless of ILT2 expression. This may lead to an underestimation of the effect of HLA-G. Thus, considering the immune subpopulations sensitive to HLA-G remained an important issue in the field. Here we present a new cytometry assay to evaluate HLA-G effects on both NK and CD8+ T cell cytotoxic functions. Using flow cytometry allows for the comparison of HLA-G function on multiple subsets and multiple func...
Source: Human Immunology - February 18, 2020 Category: Allergy & Immunology Authors: Jacquier A, Dumont C, Carosella ED, Rouas-Freiss N, LeMaoult J Tags: Hum Immunol Source Type: research

Diagnosis and treatment of immunological platelet refractoriness by histocompatibility.
Abstract Immunological platelet refractoriness occurs when polytransfused patients develop antibodies against donors' HLA class I antigens, HPA (human platelet antigens) and few cases against both systems. Flow cytometry crossmatch with the patient serum against platelets from several donors can determine whether the refractoriness is or is not of immunological origin. Patients with moderate sensitization will be given transfusions from donors with a negative platelets crossmatch; those who are hypersensitized will need to have antibodies assessed against a reactivity panel (RP) for HLA class I and HPA. The patien...
Source: Human Immunology - February 15, 2020 Category: Allergy & Immunology Authors: Fagundes IS, Franz JM, Jobim MS, Arend A, Merzoni J, Cardone JM, Gil B, Sekine L, Jobim LF Tags: Hum Immunol Source Type: research

Corrigendum to "Th9 cytokines curb cervical cancer progression and immune evasion" [80 (2019) 1020-1025].
Corrigendum to "Th9 cytokines curb cervical cancer progression and immune evasion" [80 (2019) 1020-1025]. Hum Immunol. 2020 Feb 12;: Authors: Chauhan SR, Singhal PG, Sharma U, Bandil K, Chakraborty K, Bharadwaj M PMID: 32061380 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - February 12, 2020 Category: Allergy & Immunology Authors: Chauhan SR, Singhal PG, Sharma U, Bandil K, Chakraborty K, Bharadwaj M Tags: Hum Immunol Source Type: research

Extracellular allograft inflammatory factor-1 (AIF-1) potentiates Th1 cell differentiation and inhibits Treg response in human peripheral blood mononuclear cells from normal subjects.
edo-Lobo M, Guijarro LG Abstract We identified the presence of AIF-1 (allograft inflammatory factor-1) in human peripheral blood mononuclear cells (PBMCs) from normal subjects by immunocytological methods. After isolation of different types of mononuclear cells by FACS (Fluorescence-activated cell sorting) with>95% purity, we studied the transcript levels of AIF-1 using qPCR. We observed the following order of AIF-1 mRNA expression in mononuclear cells: T-lymphocytes ˃ Monocytes ˃ B-lymphocytes ˃ NK. After T cell expansion of isolated PBMCs using anti-CD3-CD28 magnetic beads (Dy...
Source: Human Immunology - February 11, 2020 Category: Allergy & Immunology Authors: Cano-Martínez D, Monserrat J, Hernández-Breijo B, Sanmartín Salinas P, Álvarez-Mon M, Val Toledo-Lobo M, Guijarro LG Tags: Hum Immunol Source Type: research

A blueprint for electronic utilization of ambiguous molecular HLA typing data in organ allocation systems and virtual crossmatch.
Abstract Virtual crossmatch (VXM) compares a transplant candidate's unacceptable antigens to the HLA typing of the donor before an organ offer is accepted and, in selected cases, supplant a prospective physical crossmatch. However, deceased donor typing can be ambiguous, leading to uncertainty in compatibility prediction. We have developed a prototype web application that utilizes ambiguous HLA molecular typing data to predict which unacceptable antigens are present in the donor HLA genotype as donor-specific antibodies (DSA). The application compares a candidate's listed unacceptable antigens to computed probabil...
Source: Human Immunology - February 10, 2020 Category: Allergy & Immunology Authors: Kaur N, Pinelli D, Kransdorf EP, Pando MJ, Smith G, Murphey CL, Kamoun M, Bray RA, Tambur A, Gragert L Tags: Hum Immunol Source Type: research

Preface: 17th International HLA and Immunogenetics Workshop.
;a MA PMID: 32051104 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - February 9, 2020 Category: Allergy & Immunology Authors: Vayntrub TA, Mack SJ, Fernandez-Viña MA Tags: Hum Immunol Source Type: research

Siglec-10 expression is up-regulated in activated human CD4+ T cells.
Abstract Most sialic acid-binding immunoglobulin-like lectins (Siglecs) suppress immune cell function but are expressed at low levels on human T cells. We found that soluble CD52 inhibited T cell signalling by ligating Siglec-10, but the presence of Siglec-10 on human T cells has been questioned. To address this concern, we examined the expression of Siglec-10 at the RNA and protein level in human CD4+ T cells. Analysis by RNAseq, qPCR and flow cytometry demonstrated that, in contrast to other Siglecs, after activation of CD4+ T cells Siglec-10 was selectively upregulated in a subset of cells also high for CD52 ex...
Source: Human Immunology - February 8, 2020 Category: Allergy & Immunology Authors: Bandala-Sanchez E, Bediaga NG, Naselli G, Neale AM, Harrison LC Tags: Hum Immunol Source Type: research

Is the TAP2 single nucleotide polymorphism rs241447 truly associated with psoriasis in Poles?
Abstract Psoriasis vulgaris (PsV) is strongly associated with HLA-C*06:02. HLA class I molecules present antigenic peptides to CD8+ T lymphocytes. Peptide transport from cytosol to the endoplasmic reticulum is mediated by a transporter associated with antigen processing (TAP) composed of TAP1 and TAP2 polymorphic proteins. Here, we compared the distribution of three coding single nucleotide polymorphisms (SNPs), rs1057141 in TAP1 and rs1800454 and rs241447 in TAP2 as well as the HLA-C*06:02 allele in 438 patients diagnosed with PsV and 493 control individuals. In patients and controls non-stratified by HLA-C*...
Source: Human Immunology - January 30, 2020 Category: Allergy & Immunology Authors: Wiśniewski A, Wilczyńska K, Wagner M, Jasek M, Niepiekło-Miniewska W, Nowak I, Matusiak Ł, Szczerkowska-Dobosz A, Kuśnierczyk P Tags: Hum Immunol Source Type: research

Celiac disease risk stratification based on HLA-DQ heterodimer (HLA-DQA1  ~ DQB1) typing in a large cohort of adults with suspected celiac disease.
DISCUSSION: This large national reference laboratory cohort study demonstrated that HLA-DQ2.2 heterozygous is not associated with positive tTG-IgA serology, suggesting the reclassification of this haplotype as non-permissive for CeD. PMID: 32005535 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - January 28, 2020 Category: Allergy & Immunology Authors: Choung RS, Mills JR, Snyder MR, Murray JA, Gandhi MJ Tags: Hum Immunol Source Type: research

Retraction notice to "Aberrant frequency of IL-10-producing b cells and its association with Treg and MDSC cells in Non Small Cell Lung Carcinoma patients" [Hum. Immunol. 77 (1) (2016) 84-89].
Retraction notice to "Aberrant frequency of IL-10-producing b cells and its association with Treg and MDSC cells in Non Small Cell Lung Carcinoma patients" [Hum. Immunol. 77 (1) (2016) 84-89]. Hum Immunol. 2020 Jan 27;: Authors: Liu J, Yu Q, Zheng S, Jiang Y, Liu Y, Yuan G, Qiu L PMID: 32001032 [PubMed - as supplied by publisher] (Source: Human Immunology)
Source: Human Immunology - January 27, 2020 Category: Allergy & Immunology Authors: Liu J, Yu Q, Zheng S, Jiang Y, Liu Y, Yuan G, Qiu L Tags: Hum Immunol Source Type: research

The HLA-G 14-bp insertion/deletion polymorphism is associated with chronic hepatitis B in Southern Brazil: A case-control study.
Abstract There is growing evidence that the non-classical HLA-G has a role in the process of the immune response against pathogens, including HBV and HIV. Previous studies demonstrated that a 14-bp insertion/deletion (indel) polymorphism at 3'-untranslated region of HLA-G gene interferes in the mRNA stability and expression. The present study aimed to evaluate the association of the 14-bp indel polymorphism (rs371194629) with HBV infection in chronic hepatitis B (CHB) mono-infected and HBV/HIV co-infected patients from Southern Brazil. A total of 817 individuals were analyzed, including 357 CHB patients, 134 HBV/H...
Source: Human Immunology - January 16, 2020 Category: Allergy & Immunology Authors: Michel Wolf J, Zingalli Bueno Pereira VR, Zanetti Ballardin Roncato PA, Castagna Wortmann A, Stumm GZ, Oliveira da Silva F, Lunge VR, Simon D Tags: Hum Immunol Source Type: research