Circulating regulatory T cells in adult-onset Still's disease: focusing on their plasticity and stability
Clin Exp Immunol. 2021 Jul 28. doi: 10.1111/cei.13648. Online ahead of print.ABSTRACTWe investigated the characteristics of regulatory T cells in adult-onset Still's disease (AOSD), with a focus on their plasticity, stability, and relationship with disease severity. The proportion of circulating CD4+CD25+FoxP3+ cells (Tregs) and intracellular expression of effector cytokines, including interferon (IFN)-γ, interleukin (IL)-17, and IL-4, were analysed in 27 untreated patients with AOSD (acute AOSD), 11 of the 27 patients after remission, and 16 healthy controls (HC) using flow cytometry. The suppressive ability of Treg...
Source: Clinical and Developmental Immunology - July 28, 2021 Category: Allergy & Immunology Authors: Yasuhiro Shimojima Takanori Ichikawa Dai Kishida Ryota Takamatsu Yoshiki Sekijima Source Type: research

Follicular Helper and Follicular Regulatory T Cell Subsets Imbalance is Associated with higher activated B cells and Abnormal Auto-antibody Production in Primary Anti-phospholipid Syndrome Patients
Clin Exp Immunol. 2021 Jul 26. doi: 10.1111/cei.13647. Online ahead of print.ABSTRACTPrimary anti-phospholipid antibody syndrome (pAPS) is a multi-organs autoimmune disease, and autoantibodies were involved in its pathogenesis. Follicular helper T cells (Tfh) and follicular regulatory T cells (Tfr) are critical for B cells maturation and antibodies production, but their roles in pAPS remain unknown. We enrolled Thirty-two pAPS patients and twenty-three healthy controls (HCs) and comprehensively analyzed circulating Tfh and Tfr, as well as their subsets, using flow cytometry. Clinical data including autoantibody levels were...
Source: Clinical and Developmental Immunology - July 26, 2021 Category: Allergy & Immunology Authors: Yan Long Wenyi Li Jinghong Feng Yingting Ma Yuanyuan Sun Lijuan Xu Ying Song Chen Liu Source Type: research

CD4 < sup > + < /sup > LAG-3 < sup > + < /sup > T Cells are Decreased in Active Psoriatic Arthritis Patients and Their Restoration In Vitro is Mediated by TNF Inhibitors
Clin Exp Immunol. 2021 Jul 26. doi: 10.1111/cei.13646. Online ahead of print.ABSTRACTPsoriatic arthritis (PsA) is a chronic inflammatory disease associated with T cell dysregulation. The lymphocyte-activation gene (LAG)-3 is one of the regulatory receptors expressed on T cells and in a soluble form. LAG-3 expression on T cells was analyzed in vitro in PsA patients with minimal disease activity (MDA), active disease (non-MDA), and healthy controls. In cultured in vitro peripheral blood mononuclear cells (PBMCs), LAG-3 expression on CD4+ T cells was similar in both MDA PsA patients (7.5 ± 0.9) (n=14) and healthy contr...
Source: Clinical and Developmental Immunology - July 26, 2021 Category: Allergy & Immunology Authors: Smadar Gertel Ari Polachek Victoria Furer David Levartovsky Ori Elkayam Source Type: research

Clinical and laboratory findings in patients with leukocyte adhesion deficiency type I: A multicenter study in turkey
We present clinical and immunological features of 15 patients with leukocyte adhesion deficiency type I (LAD-I). Targeted next-generation sequencing was performed with either a primary immunodeficiency gene panel comprising 266 genes or a small LAD-panel consisting of five genes for genetic analysis. To measure the expression level of integrins on the leukocyte surface, flow cytometry analysis was performed. The median age of the patients at diagnosis was 3 (1-48) months. Eleven (73%) of the 15 patients had LAD-I diagnosis in their first 6 months, and fourteen (93%) patients had consanguineous parents. Delayed separation o...
Source: Clinical and Developmental Immunology - July 26, 2021 Category: Allergy & Immunology Authors: Ismail Yaz Begum Ozbek Hacer Neslihan Bildik Cagman Tan Sevil Oskay Halacli Elif Soyak Aytekin Saliha Esenboga Sukru Cekic Sebnem Sara Kilic Ozlem Keskin Karin van Leeuwen Dirk Roos Deniz Cagdas Ilhan Tezcan Source Type: research

Follicular Helper and Follicular Regulatory T Cell Subsets Imbalance is Associated with higher activated B cells and Abnormal Auto-antibody Production in Primary Anti-phospholipid Syndrome Patients
Clin Exp Immunol. 2021 Jul 26. doi: 10.1111/cei.13647. Online ahead of print.ABSTRACTPrimary anti-phospholipid antibody syndrome (pAPS) is a multi-organs autoimmune disease, and autoantibodies were involved in its pathogenesis. Follicular helper T cells (Tfh) and follicular regulatory T cells (Tfr) are critical for B cells maturation and antibodies production, but their roles in pAPS remain unknown. We enrolled Thirty-two pAPS patients and twenty-three healthy controls (HCs) and comprehensively analyzed circulating Tfh and Tfr, as well as their subsets, using flow cytometry. Clinical data including autoantibody levels were...
Source: Clinical and Developmental Immunology - July 26, 2021 Category: Allergy & Immunology Authors: Yan Long Wenyi Li Jinghong Feng Yingting Ma Yuanyuan Sun Lijuan Xu Ying Song Chen Liu Source Type: research

CD4 < sup > + < /sup > LAG-3 < sup > + < /sup > T Cells are Decreased in Active Psoriatic Arthritis Patients and Their Restoration In Vitro is Mediated by TNF Inhibitors
Clin Exp Immunol. 2021 Jul 26. doi: 10.1111/cei.13646. Online ahead of print.ABSTRACTPsoriatic arthritis (PsA) is a chronic inflammatory disease associated with T cell dysregulation. The lymphocyte-activation gene (LAG)-3 is one of the regulatory receptors expressed on T cells and in a soluble form. LAG-3 expression on T cells was analyzed in vitro in PsA patients with minimal disease activity (MDA), active disease (non-MDA), and healthy controls. In cultured in vitro peripheral blood mononuclear cells (PBMCs), LAG-3 expression on CD4+ T cells was similar in both MDA PsA patients (7.5 ± 0.9) (n=14) and healthy contr...
Source: Clinical and Developmental Immunology - July 26, 2021 Category: Allergy & Immunology Authors: Smadar Gertel Ari Polachek Victoria Furer David Levartovsky Ori Elkayam Source Type: research

Clinical and laboratory findings in patients with leukocyte adhesion deficiency type I: A multicenter study in turkey
We present clinical and immunological features of 15 patients with leukocyte adhesion deficiency type I (LAD-I). Targeted next-generation sequencing was performed with either a primary immunodeficiency gene panel comprising 266 genes or a small LAD-panel consisting of five genes for genetic analysis. To measure the expression level of integrins on the leukocyte surface, flow cytometry analysis was performed. The median age of the patients at diagnosis was 3 (1-48) months. Eleven (73%) of the 15 patients had LAD-I diagnosis in their first 6 months, and fourteen (93%) patients had consanguineous parents. Delayed separation o...
Source: Clinical and Developmental Immunology - July 26, 2021 Category: Allergy & Immunology Authors: Ismail Yaz Begum Ozbek Hacer Neslihan Bildik Cagman Tan Sevil Oskay Halacli Elif Soyak Aytekin Saliha Esenboga Sukru Cekic Sebnem Sara Kilic Ozlem Keskin Karin van Leeuwen Dirk Roos Deniz Cagdas Ilhan Tezcan Source Type: research

Differences in cytokine and chemokine profiles in cerebrospinal fluid caused by the etiology of cryptococcal meningitis and tuberculous meningitis in HIV patients
Clin Exp Immunol. 2021 Jul 21. doi: 10.1111/cei.13644. Online ahead of print.ABSTRACTThe roles of cytokines and chemokines in HIV-associated cryptococcal meningitis (HCM) and HIV-associated tuberculous meningitis (HTBM) are debatable. In sum, 34 HIV-infected patients without meningitis, 44 HCM patients and 27 HTBM patients were enrolled for study. The concentrations of 22 cytokines/chemokines in cerebrospinal fluid (CSF) were assayed in at admission. Principal component analysis (PCA), Pearson and logistic regression analyses were used to assess the role of cytokines/chemokines in HCM and HTBM. We found the levels of Th17,...
Source: Clinical and Developmental Immunology - July 21, 2021 Category: Allergy & Immunology Authors: Lijun Xu Yufan Xu Yanghao Zheng Xiuming Peng Zongxing Yang Qing Cao Dairong Xiang Handan Zhao Source Type: research

Differences in cytokine and chemokine profiles in cerebrospinal fluid caused by the etiology of cryptococcal meningitis and tuberculous meningitis in HIV patients
Clin Exp Immunol. 2021 Jul 21. doi: 10.1111/cei.13644. Online ahead of print.ABSTRACTThe roles of cytokines and chemokines in HIV-associated cryptococcal meningitis (HCM) and HIV-associated tuberculous meningitis (HTBM) are debatable. In sum, 34 HIV-infected patients without meningitis, 44 HCM patients and 27 HTBM patients were enrolled for study. The concentrations of 22 cytokines/chemokines in cerebrospinal fluid (CSF) were assayed in at admission. Principal component analysis (PCA), Pearson and logistic regression analyses were used to assess the role of cytokines/chemokines in HCM and HTBM. We found the levels of Th17,...
Source: Clinical and Developmental Immunology - July 21, 2021 Category: Allergy & Immunology Authors: Lijun Xu Yufan Xu Yanghao Zheng Xiuming Peng Zongxing Yang Qing Cao Dairong Xiang Handan Zhao Source Type: research

Differences in cytokine and chemokine profiles in cerebrospinal fluid caused by the etiology of cryptococcal meningitis and tuberculous meningitis in HIV patients
Clin Exp Immunol. 2021 Jul 21. doi: 10.1111/cei.13644. Online ahead of print.ABSTRACTThe roles of cytokines and chemokines in HIV-associated cryptococcal meningitis (HCM) and HIV-associated tuberculous meningitis (HTBM) are debatable. In sum, 34 HIV-infected patients without meningitis, 44 HCM patients and 27 HTBM patients were enrolled for study. The concentrations of 22 cytokines/chemokines in cerebrospinal fluid (CSF) were assayed in at admission. Principal component analysis (PCA), Pearson and logistic regression analyses were used to assess the role of cytokines/chemokines in HCM and HTBM. We found the levels of Th17,...
Source: Clinical and Developmental Immunology - July 21, 2021 Category: Allergy & Immunology Authors: Lijun Xu Yufan Xu Yanghao Zheng Xiuming Peng Zongxing Yang Qing Cao Dairong Xiang Handan Zhao Source Type: research

Differences in cytokine and chemokine profiles in cerebrospinal fluid caused by the etiology of cryptococcal meningitis and tuberculous meningitis in HIV patients
Clin Exp Immunol. 2021 Jul 21. doi: 10.1111/cei.13644. Online ahead of print.ABSTRACTThe roles of cytokines and chemokines in HIV-associated cryptococcal meningitis (HCM) and HIV-associated tuberculous meningitis (HTBM) are debatable. In sum, 34 HIV-infected patients without meningitis, 44 HCM patients and 27 HTBM patients were enrolled for study. The concentrations of 22 cytokines/chemokines in cerebrospinal fluid (CSF) were assayed in at admission. Principal component analysis (PCA), Pearson and logistic regression analyses were used to assess the role of cytokines/chemokines in HCM and HTBM. We found the levels of Th17,...
Source: Clinical and Developmental Immunology - July 21, 2021 Category: Allergy & Immunology Authors: Lijun Xu Yufan Xu Yanghao Zheng Xiuming Peng Zongxing Yang Qing Cao Dairong Xiang Handan Zhao Source Type: research

Differences in cytokine and chemokine profiles in cerebrospinal fluid caused by the etiology of cryptococcal meningitis and tuberculous meningitis in HIV patients
Clin Exp Immunol. 2021 Jul 21. doi: 10.1111/cei.13644. Online ahead of print.ABSTRACTThe roles of cytokines and chemokines in HIV-associated cryptococcal meningitis (HCM) and HIV-associated tuberculous meningitis (HTBM) are debatable. In sum, 34 HIV-infected patients without meningitis, 44 HCM patients and 27 HTBM patients were enrolled for study. The concentrations of 22 cytokines/chemokines in cerebrospinal fluid (CSF) were assayed in at admission. Principal component analysis (PCA), Pearson and logistic regression analyses were used to assess the role of cytokines/chemokines in HCM and HTBM. We found the levels of Th17,...
Source: Clinical and Developmental Immunology - July 21, 2021 Category: Allergy & Immunology Authors: Lijun Xu Yufan Xu Yanghao Zheng Xiuming Peng Zongxing Yang Qing Cao Dairong Xiang Handan Zhao Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Impaired function of PD-1+ follicular regulatory T cells in systemic lupus erythematosus
Clin Exp Immunol. 2021 Jul 9. doi: 10.1111/cei.13643. Online ahead of print.ABSTRACTAberrant autoantibody production is characteristic of systemic lupus erythematosus (SLE), but follicular regulatory T (TFR) cells potentially can suppress this abnormality. We investigate functional changes in TFR cells from SLE patients. Circulating TFR cells were collected from 19 SLE patients and 14 healthy controls (HC) to compare molecular expression and in vitro suppressive capacity of follicular helper T (TFH) cell proliferation. To reveal the stability of Foxp3 in TFR, pyrosequencing of conserved non-coding sequence (CNS) 2 at the F...
Source: Clinical and Developmental Immunology - July 9, 2021 Category: Allergy & Immunology Authors: Izumi Kurata Natsuko Mikami Ayako Ohyama Atsumu Osada Yuya Kondo Hiroto Tsuboi Takayuki Sumida Isao Matsumoto Source Type: research

Newborn screening for severe combined immunodeficiency-Coming to a region near you soon
Clin Exp Immunol. 2021 Jul 8. doi: 10.1111/cei.13642. Online ahead of print.ABSTRACTThe most profound of primary immunodeficiencies, severe combined immunodeficiency (SCID), presents in infancy. Infants appear healthy at birth, but they are unable to clear pathogens, particularly viruses, and present with recurrent infection, progressive pnueumonitis and failure to thrive due to enteric viral infection, often associated with persistent vaccine-strain rotavirus. The administration of live vaccines is contraindicated in these infants, but most who are eligible receive bacillus Calmette-Guérin vaccination and the live ...
Source: Clinical and Developmental Immunology - July 8, 2021 Category: Allergy & Immunology Authors: David A C Elliman Andrew R Gennery Source Type: research

Newborn screening for severe combined immunodeficiency-Coming to a region near you soon
Clin Exp Immunol. 2021 Jul 8. doi: 10.1111/cei.13642. Online ahead of print.ABSTRACTThe most profound of primary immunodeficiencies, severe combined immunodeficiency (SCID), presents in infancy. Infants appear healthy at birth, but they are unable to clear pathogens, particularly viruses, and present with recurrent infection, progressive pnueumonitis and failure to thrive due to enteric viral infection, often associated with persistent vaccine-strain rotavirus. The administration of live vaccines is contraindicated in these infants, but most who are eligible receive bacillus Calmette-Guérin vaccination and the live ...
Source: Clinical and Developmental Immunology - July 8, 2021 Category: Allergy & Immunology Authors: David A C Elliman Andrew R Gennery Source Type: research

Prognostic implications of tumor infiltrating lymphocytes for recurrence in epithelial ovarian cancer
Clin Exp Immunol. 2021 Jun 30. doi: 10.1111/cei.13639. Online ahead of print.ABSTRACTThe recurrence of patients with epithelial ovarian cancer (EOC) is largely attributed to tumour cells escaping from the surveillance of immune cells. However, to date there are a lack of studies that have systematically evaluated the associations between the infiltration fraction of immune cells and the recurrence risk of EOC. Based on the microRNA expression profiles of 441 EOC patients, we constructed a microRNA-based panel with recurrence prediction potential using nonnegative matrix factorization consensus clustering. Then, we evaluate...
Source: Clinical and Developmental Immunology - July 1, 2021 Category: Allergy & Immunology Authors: Yuan Wu Yue Gao Lingxi Chen Xin Jin Pingbo Chen Qingqing Mo Source Type: research

Prognostic implications of tumor infiltrating lymphocytes for recurrence in epithelial ovarian cancer
Clin Exp Immunol. 2021 Jun 30. doi: 10.1111/cei.13639. Online ahead of print.ABSTRACTThe recurrence of patients with epithelial ovarian cancer (EOC) is largely attributed to tumour cells escaping from the surveillance of immune cells. However, to date there are a lack of studies that have systematically evaluated the associations between the infiltration fraction of immune cells and the recurrence risk of EOC. Based on the microRNA expression profiles of 441 EOC patients, we constructed a microRNA-based panel with recurrence prediction potential using nonnegative matrix factorization consensus clustering. Then, we evaluate...
Source: Clinical and Developmental Immunology - July 1, 2021 Category: Allergy & Immunology Authors: Yuan Wu Yue Gao Lingxi Chen Xin Jin Pingbo Chen Qingqing Mo Source Type: research

Prognostic implications of tumor infiltrating lymphocytes for recurrence in epithelial ovarian cancer
Clin Exp Immunol. 2021 Jun 30. doi: 10.1111/cei.13639. Online ahead of print.ABSTRACTThe recurrence of patients with epithelial ovarian cancer (EOC) is largely attributed to tumour cells escaping from the surveillance of immune cells. However, to date there are a lack of studies that have systematically evaluated the associations between the infiltration fraction of immune cells and the recurrence risk of EOC. Based on the microRNA expression profiles of 441 EOC patients, we constructed a microRNA-based panel with recurrence prediction potential using nonnegative matrix factorization consensus clustering. Then, we evaluate...
Source: Clinical and Developmental Immunology - July 1, 2021 Category: Allergy & Immunology Authors: Yuan Wu Yue Gao Lingxi Chen Xin Jin Pingbo Chen Qingqing Mo Source Type: research

Prognostic implications of tumor infiltrating lymphocytes for recurrence in epithelial ovarian cancer
Clin Exp Immunol. 2021 Jun 30. doi: 10.1111/cei.13639. Online ahead of print.ABSTRACTThe recurrence of patients with epithelial ovarian cancer (EOC) is largely attributed to tumour cells escaping from the surveillance of immune cells. However, to date there are a lack of studies that have systematically evaluated the associations between the infiltration fraction of immune cells and the recurrence risk of EOC. Based on the microRNA expression profiles of 441 EOC patients, we constructed a microRNA-based panel with recurrence prediction potential using nonnegative matrix factorization consensus clustering. Then, we evaluate...
Source: Clinical and Developmental Immunology - July 1, 2021 Category: Allergy & Immunology Authors: Yuan Wu Yue Gao Lingxi Chen Xin Jin Pingbo Chen Qingqing Mo Source Type: research

Prognostic implications of tumor infiltrating lymphocytes for recurrence in epithelial ovarian cancer
Clin Exp Immunol. 2021 Jun 30. doi: 10.1111/cei.13639. Online ahead of print.ABSTRACTThe recurrence of patients with epithelial ovarian cancer (EOC) is largely attributed to tumour cells escaping from the surveillance of immune cells. However, to date there are a lack of studies that have systematically evaluated the associations between the infiltration fraction of immune cells and the recurrence risk of EOC. Based on the microRNA expression profiles of 441 EOC patients, we constructed a microRNA-based panel with recurrence prediction potential using nonnegative matrix factorization consensus clustering. Then, we evaluate...
Source: Clinical and Developmental Immunology - July 1, 2021 Category: Allergy & Immunology Authors: Yuan Wu Yue Gao Lingxi Chen Xin Jin Pingbo Chen Qingqing Mo Source Type: research

Prognostic implications of tumor infiltrating lymphocytes for recurrence in epithelial ovarian cancer
Clin Exp Immunol. 2021 Jun 30. doi: 10.1111/cei.13639. Online ahead of print.ABSTRACTThe recurrence of patients with epithelial ovarian cancer (EOC) is largely attributed to tumour cells escaping from the surveillance of immune cells. However, to date there are a lack of studies that have systematically evaluated the associations between the infiltration fraction of immune cells and the recurrence risk of EOC. Based on the microRNA expression profiles of 441 EOC patients, we constructed a microRNA-based panel with recurrence prediction potential using nonnegative matrix factorization consensus clustering. Then, we evaluate...
Source: Clinical and Developmental Immunology - July 1, 2021 Category: Allergy & Immunology Authors: Yuan Wu Yue Gao Lingxi Chen Xin Jin Pingbo Chen Qingqing Mo Source Type: research

Corrigendum
Clin Exp Immunol. 2021 Jun 28. doi: 10.1111/cei.13621. Online ahead of print.NO ABSTRACTPMID:34184255 | DOI:10.1111/cei.13621 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 29, 2021 Category: Allergy & Immunology Source Type: research

Corrigendum
Clin Exp Immunol. 2021 Jun 28. doi: 10.1111/cei.13621. Online ahead of print.NO ABSTRACTPMID:34184255 | DOI:10.1111/cei.13621 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 29, 2021 Category: Allergy & Immunology Source Type: research

Clinical and Neuroimaging Findings in MOGAD - MRI and OCT
We describe how these imaging techniques may be used to study this group of disorders and discuss how image analysis methods have led to recent insights for consideration in future studies.PMID:34152000 | DOI:10.1111/cei.13641 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 21, 2021 Category: Allergy & Immunology Authors: F Bartels A Lu F C Oertel C Finke F Paul C Chien Source Type: research

Clinical and Neuroimaging Findings in MOGAD - MRI and OCT
We describe how these imaging techniques may be used to study this group of disorders and discuss how image analysis methods have led to recent insights for consideration in future studies.PMID:34152000 | DOI:10.1111/cei.13641 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 21, 2021 Category: Allergy & Immunology Authors: F Bartels A Lu F C Oertel C Finke F Paul C Chien Source Type: research

Clinical and Neuroimaging Findings in MOGAD - MRI and OCT
We describe how these imaging techniques may be used to study this group of disorders and discuss how image analysis methods have led to recent insights for consideration in future studies.PMID:34152000 | DOI:10.1111/cei.13641 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 21, 2021 Category: Allergy & Immunology Authors: F Bartels A Lu F C Oertel C Finke F Paul C Chien Source Type: research

Clinical and Neuroimaging Findings in MOGAD - MRI and OCT
We describe how these imaging techniques may be used to study this group of disorders and discuss how image analysis methods have led to recent insights for consideration in future studies.PMID:34152000 | DOI:10.1111/cei.13641 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 21, 2021 Category: Allergy & Immunology Authors: F Bartels A Lu F C Oertel C Finke F Paul C Chien Source Type: research

Clinical and Neuroimaging Findings in MOGAD - MRI and OCT
We describe how these imaging techniques may be used to study this group of disorders and discuss how image analysis methods have led to recent insights for consideration in future studies.PMID:34152000 | DOI:10.1111/cei.13641 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 21, 2021 Category: Allergy & Immunology Authors: F Bartels A Lu F C Oertel C Finke F Paul C Chien Source Type: research

Clinical and Neuroimaging Findings in MOGAD - MRI and OCT
We describe how these imaging techniques may be used to study this group of disorders and discuss how image analysis methods have led to recent insights for consideration in future studies.PMID:34152000 | DOI:10.1111/cei.13641 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 21, 2021 Category: Allergy & Immunology Authors: F Bartels A Lu F C Oertel C Finke F Paul C Chien Source Type: research

Clinical and Neuroimaging Findings in MOGAD - MRI and OCT
We describe how these imaging techniques may be used to study this group of disorders and discuss how image analysis methods have led to recent insights for consideration in future studies.PMID:34152000 | DOI:10.1111/cei.13641 (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - June 21, 2021 Category: Allergy & Immunology Authors: F Bartels A Lu F C Oertel C Finke F Paul C Chien Source Type: research

Cytometric analysis of T cell phenotype using cytokine profiling for improved manufacturing of an EBV-specific T cell therapy
We describe improved GMP-compliant culture and analysis processes for conventional lymphoblastoid cell line (LCL)-driven EBV-specific T cell manufacture, and describe an improved phenotyping approach for analysing T cell products. We optimised the current LCL-mediated clinical manufacture of EBV-specific T cells to establish an improved process using xenoprotein-free GMP-compliant reagents throughout, and compared resulting products with our previous banked T cell clinical therapy. We assessed effects of changes to LCL: T cell ratio in T cell expansion, and developed a robust flow cytometric marker panel covering T cell me...
Source: Clinical and Developmental Immunology - June 19, 2021 Category: Allergy & Immunology Authors: Rachel S Cooper Aleksandra Kowalczuk Gwen Wilkie Mark A Vickers Marc L Turner John D M Campbell Alasdair R Fraser Source Type: research

Cytometric analysis of T cell phenotype using cytokine profiling for improved manufacturing of an EBV-specific T cell therapy
We describe improved GMP-compliant culture and analysis processes for conventional lymphoblastoid cell line (LCL)-driven EBV-specific T cell manufacture, and describe an improved phenotyping approach for analysing T cell products. We optimised the current LCL-mediated clinical manufacture of EBV-specific T cells to establish an improved process using xenoprotein-free GMP-compliant reagents throughout, and compared resulting products with our previous banked T cell clinical therapy. We assessed effects of changes to LCL: T cell ratio in T cell expansion, and developed a robust flow cytometric marker panel covering T cell me...
Source: Clinical and Developmental Immunology - June 19, 2021 Category: Allergy & Immunology Authors: Rachel S Cooper Aleksandra Kowalczuk Gwen Wilkie Mark A Vickers Marc L Turner John D M Campbell Alasdair R Fraser Source Type: research

Assessment of bacterial exposure on phagocytic capability and surface marker expression of sputum macrophages and neutrophils in COPD patients
Clin Exp Immunol. 2021 Jun 18. doi: 10.1111/cei.13638. Online ahead of print.ABSTRACTDefective phagocytosis has been shown in COPD bronchoalveolar lavage and blood monocyte derived macrophages. Phagocytic capabilities of sputum macrophages and neutrophils in COPD are unknown. We investigated phagocytosis in these cells from COPD patients and controls. Phagocytosis of Streptococcus pneumoniae or fluorescently labelled non-typeable Haemophilus influenzae (NTHi) by sputum macrophages and neutrophils was determined by gentamycin protection assay (COPD; n=5) or flow cytometry in 14 COPD patients, 8 healthy smokers (HS) and 9 he...
Source: Clinical and Developmental Immunology - June 18, 2021 Category: Allergy & Immunology Authors: Simon Lea Rosemary Gaskell Simon Hall Barbara Maschera Edith Hessel Dave Singh Source Type: research

Assessment of bacterial exposure on phagocytic capability and surface marker expression of sputum macrophages and neutrophils in COPD patients
Clin Exp Immunol. 2021 Jun 18. doi: 10.1111/cei.13638. Online ahead of print.ABSTRACTDefective phagocytosis has been shown in COPD bronchoalveolar lavage and blood monocyte derived macrophages. Phagocytic capabilities of sputum macrophages and neutrophils in COPD are unknown. We investigated phagocytosis in these cells from COPD patients and controls. Phagocytosis of Streptococcus pneumoniae or fluorescently labelled non-typeable Haemophilus influenzae (NTHi) by sputum macrophages and neutrophils was determined by gentamycin protection assay (COPD; n=5) or flow cytometry in 14 COPD patients, 8 healthy smokers (HS) and 9 he...
Source: Clinical and Developmental Immunology - June 18, 2021 Category: Allergy & Immunology Authors: Simon Lea Rosemary Gaskell Simon Hall Barbara Maschera Edith Hessel Dave Singh Source Type: research

NF- κB inhibitor suppresses experimental autoimmune neuritis in mice via declining macrophages polarization to M1 type
Clin Exp Immunol. 2021 Jun 12. doi: 10.1111/cei.13637. Online ahead of print.ABSTRACTGuillain-Barre' syndrome (GBS) is an acute inflammatory and immune-mediated demyelinating disease of peripheral nervous system (PNS). Macrophages play a central role in its animal model, experimental autoimmune neuritis (EAN), which has been well-accepted. Additionally, NF-κB inhibitors has been used to treat cancers and showed beneficial effects. Here we investigated the therapeutic effect of M2 macrophage and NF-κB pathway's correlation with macrophages activation in EAN in C57BL/6 mice. We demonstrated that M2 macrophage tra...
Source: Clinical and Developmental Immunology - June 12, 2021 Category: Allergy & Immunology Authors: Donghui Shen Fengna Chu Yue Lang Chao Zheng Chunrong Li Kangding Liu Jie Zhu Source Type: research

NF- κB inhibitor suppresses experimental autoimmune neuritis in mice via declining macrophages polarization to M1 type
Clin Exp Immunol. 2021 Jun 12. doi: 10.1111/cei.13637. Online ahead of print.ABSTRACTGuillain-Barre' syndrome (GBS) is an acute inflammatory and immune-mediated demyelinating disease of peripheral nervous system (PNS). Macrophages play a central role in its animal model, experimental autoimmune neuritis (EAN), which has been well-accepted. Additionally, NF-κB inhibitors has been used to treat cancers and showed beneficial effects. Here we investigated the therapeutic effect of M2 macrophage and NF-κB pathway's correlation with macrophages activation in EAN in C57BL/6 mice. We demonstrated that M2 macrophage tra...
Source: Clinical and Developmental Immunology - June 12, 2021 Category: Allergy & Immunology Authors: Donghui Shen Fengna Chu Yue Lang Chao Zheng Chunrong Li Kangding Liu Jie Zhu Source Type: research

NF- κB inhibitor suppresses experimental autoimmune neuritis in mice via declining macrophages polarization to M1 type
Clin Exp Immunol. 2021 Jun 12. doi: 10.1111/cei.13637. Online ahead of print.ABSTRACTGuillain-Barre' syndrome (GBS) is an acute inflammatory and immune-mediated demyelinating disease of peripheral nervous system (PNS). Macrophages play a central role in its animal model, experimental autoimmune neuritis (EAN), which has been well-accepted. Additionally, NF-κB inhibitors has been used to treat cancers and showed beneficial effects. Here we investigated the therapeutic effect of M2 macrophage and NF-κB pathway's correlation with macrophages activation in EAN in C57BL/6 mice. We demonstrated that M2 macrophage tra...
Source: Clinical and Developmental Immunology - June 12, 2021 Category: Allergy & Immunology Authors: Donghui Shen Fengna Chu Yue Lang Chao Zheng Chunrong Li Kangding Liu Jie Zhu Source Type: research

NF- κB inhibitor suppresses experimental autoimmune neuritis in mice via declining macrophages polarization to M1 type
Clin Exp Immunol. 2021 Jun 12. doi: 10.1111/cei.13637. Online ahead of print.ABSTRACTGuillain-Barre' syndrome (GBS) is an acute inflammatory and immune-mediated demyelinating disease of peripheral nervous system (PNS). Macrophages play a central role in its animal model, experimental autoimmune neuritis (EAN), which has been well-accepted. Additionally, NF-κB inhibitors has been used to treat cancers and showed beneficial effects. Here we investigated the therapeutic effect of M2 macrophage and NF-κB pathway's correlation with macrophages activation in EAN in C57BL/6 mice. We demonstrated that M2 macrophage tra...
Source: Clinical and Developmental Immunology - June 12, 2021 Category: Allergy & Immunology Authors: Donghui Shen Fengna Chu Yue Lang Chao Zheng Chunrong Li Kangding Liu Jie Zhu Source Type: research

NF- κB inhibitor suppresses experimental autoimmune neuritis in mice via declining macrophages polarization to M1 type
Clin Exp Immunol. 2021 Jun 12. doi: 10.1111/cei.13637. Online ahead of print.ABSTRACTGuillain-Barre' syndrome (GBS) is an acute inflammatory and immune-mediated demyelinating disease of peripheral nervous system (PNS). Macrophages play a central role in its animal model, experimental autoimmune neuritis (EAN), which has been well-accepted. Additionally, NF-κB inhibitors has been used to treat cancers and showed beneficial effects. Here we investigated the therapeutic effect of M2 macrophage and NF-κB pathway's correlation with macrophages activation in EAN in C57BL/6 mice. We demonstrated that M2 macrophage tra...
Source: Clinical and Developmental Immunology - June 12, 2021 Category: Allergy & Immunology Authors: Donghui Shen Fengna Chu Yue Lang Chao Zheng Chunrong Li Kangding Liu Jie Zhu Source Type: research

Chronic Demodicosis in Patients with Immune Dysregulation: An Unexpected Infectious Manifestation of Signal Transducer and Activator of Transcription (STAT)1 Gain-of-Function
Clin Exp Immunol. 2021 Jun 11. doi: 10.1111/cei.13636. Online ahead of print.ABSTRACTSignal transducer and activator of transcription (STAT)1 heterozygous gain-of-function (GOF) mutations are known to induce immune dysregulation and chronic mucocutaneous candidiasis (CMCC). Previous reports suggest an association between demodicosis and STAT1 GOF. However, immune characterization of these patients is lacking. Here, we present a retrospective analysis of patients with immune dysregulation and STAT1 GOF who presented with facial and ocular demodicosis. In-depth immune phenotyping and functional studies were used to character...
Source: Clinical and Developmental Immunology - June 11, 2021 Category: Allergy & Immunology Authors: Oded Shamriz Atar Lev Amos J Simon Ortal Barel Elisheva Javasky Sigal Matza-Porges Adir Shaulov Zev Davidovics Ori Toker Raz Somech Abraham Zlotogorski Vered Molho-Pessach Yuval Tal Source Type: research

Chronic Demodicosis in Patients with Immune Dysregulation: An Unexpected Infectious Manifestation of Signal Transducer and Activator of Transcription (STAT)1 Gain-of-Function
Clin Exp Immunol. 2021 Jun 11. doi: 10.1111/cei.13636. Online ahead of print.ABSTRACTSignal transducer and activator of transcription (STAT)1 heterozygous gain-of-function (GOF) mutations are known to induce immune dysregulation and chronic mucocutaneous candidiasis (CMCC). Previous reports suggest an association between demodicosis and STAT1 GOF. However, immune characterization of these patients is lacking. Here, we present a retrospective analysis of patients with immune dysregulation and STAT1 GOF who presented with facial and ocular demodicosis. In-depth immune phenotyping and functional studies were used to character...
Source: Clinical and Developmental Immunology - June 11, 2021 Category: Allergy & Immunology Authors: Oded Shamriz Atar Lev Amos J Simon Ortal Barel Elisheva Javasky Sigal Matza-Porges Adir Shaulov Zev Davidovics Ori Toker Raz Somech Abraham Zlotogorski Vered Molho-Pessach Yuval Tal Source Type: research

A myriad of roles of dendritic cells in atherosclerosis
Clin Exp Immunol. 2021 Jun 10. doi: 10.1111/cei.13634. Online ahead of print.ABSTRACTAtherosclerosis is an inflammatory disease with break-down of homeostatic immune regulation of vascular tissues. As critical initiator of host immunity, dendritic cells (DCs) have been identified in the aorta of healthy individuals and atherosclerotic patients as well, whose roles in regulating the arterial inflammation aroused great interests. Accumulating evidences have now pointed to the fundamental roles for DCs in every developmental stages of atherosclerosis due to their myriads of functions in immunity and tolerance induction rangin...
Source: Clinical and Developmental Immunology - June 10, 2021 Category: Allergy & Immunology Authors: Yanfang Zhao Jing Zhang Wenjie Zhang Yuekang Xu Source Type: research