Immune complexome analysis reveals the presence of immune complexes and identifies disease-specific immune complex antigens in saliva samples from patients with Sj ögren's syndrome.
Immune complexome analysis reveals the presence of immune complexes and identifies disease-specific immune complex antigens in saliva samples from patients with Sjögren's syndrome. Clin Exp Immunol. 2021 Jan 12;: Authors: Yamane K, Nakamura H, Hamasaki M, Minei Y, Aibara N, Shimizu T, Kawakami A, Nakashima M, Kuroda N, Ohyama K Abstract Sjögren's syndrome (SS) is a chronic autoimmune disease that mainly damages the salivary and lacrimal glands. Immune complex (IC) formation triggers local inflammation through IC deposition and decreased antigen function. Some ICs can leak from the lesion and...
Source: Clinical and Developmental Immunology - January 12, 2021 Category: Allergy & Immunology Authors: Yamane K, Nakamura H, Hamasaki M, Minei Y, Aibara N, Shimizu T, Kawakami A, Nakashima M, Kuroda N, Ohyama K Tags: Clin Exp Immunol Source Type: research

Adoptive Transfer of Immunomodulatory M2 Macrophages Suppresses Experimental Autoimmune Encephalomyelitis in C57BL/6 Mice via Blockading NF- κB Pathway.
In this study we elucidated the roles of M1 and M2 macrophages in the pathogenesis of EAE and the effects of treatment with M2 macrophages that target certain pro-inflammatory cytokines and with immunomodulatory preparations that beneficially influence the disease course. We found macrophages increased at the onset of clinical signs in the EAE group, consistent with an increased proportion of M1 macrophages and low numbers of M2 macrophages. As the disease progressed and the symptoms worsened, M1 macrophages were decreased and M2 macrophages were gradually increased till the peak. In the recovery stage, M2 macrophages were...
Source: Clinical and Developmental Immunology - January 11, 2021 Category: Allergy & Immunology Authors: Chu F, Shi M, Lang Y, Chao Z, Jin T, Cui L, Zhu J Tags: Clin Exp Immunol Source Type: research

IRF5 regulates airway macrophage metabolic responses.
Abstract Interferon regulatory factor 5 (IRF5) is a master regulator of macrophage phenotype and a key transcription factor involved in expression of pro-inflammatory cytokine responses to microbial and viral infection. Here, we show that IRF5 controls cellular and metabolic responses. By integrating ChIP-seq and ATAC-seq datasets, we uncovered that IRF5 directly regulates metabolic genes such as hexokinase-2 (Hk2). The interaction of IRF5 and metabolic genes had a functional consequence, since Irf5-/- AMs but not bone marrow derived macrophages (BMDMs) were characterized by a quiescent metabolic phenotype at base...
Source: Clinical and Developmental Immunology - January 10, 2021 Category: Allergy & Immunology Authors: Albers GJ, Iwasaki J, McErlean P, Ogger PP, Ghai P, Khoyratty TE, Udalova IA, Lloyd CM, Byrne AJ Tags: Clin Exp Immunol Source Type: research

Beh çet disease, New insights in disease associations and manifestations. A next generation sequencing study.
Behçet disease, New insights in disease associations and manifestations. A next generation sequencing study. Clin Exp Immunol. 2021 Jan 09;: Authors: Elfishawi M, Mossallam G, Augusto D, Montero-Martin G, de Bruin H, Van de Pasch L, Norman PJ, Rozemuller E, Fernandez-Vina M, Abrudescu A, Hollenbach JA, Zaky K, Elfishawi S Abstract Behçet disease is a multisystem disease associated with Human Leucocyte Antigen (HLA) class I polymorphism. High-resolution Next Generation Sequencing (NGS) with haplotype analysis has not been performed previously for this disease. Sixty Egyptian patients diag...
Source: Clinical and Developmental Immunology - January 9, 2021 Category: Allergy & Immunology Authors: Elfishawi M, Mossallam G, Augusto D, Montero-Martin G, de Bruin H, Van de Pasch L, Norman PJ, Rozemuller E, Fernandez-Vina M, Abrudescu A, Hollenbach JA, Zaky K, Elfishawi S Tags: Clin Exp Immunol Source Type: research

Clinical & Experimental Immunology: Highlights of 2020.
Clinical & Experimental Immunology: Highlights of 2020. Clin Exp Immunol. 2021 Jan;203(1):1-2 Authors: Taams LS, Taylor RS PMID: 33330972 [PubMed - in process] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - December 18, 2020 Category: Allergy & Immunology Authors: Taams LS, Taylor RS Tags: Clin Exp Immunol Source Type: research

Corrigendum.
Authors: PMID: 33330973 [PubMed] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - December 18, 2020 Category: Allergy & Immunology Tags: Clin Exp Immunol Source Type: research

Immunological basis of early clearance of Mycobacterium tuberculosis infection: the role of natural killer cells.
Abstract Tuberculosis kills more people than any other single infectious disease globally. Despite decades of research, there is no vaccine to prevent TB transmission. Bacille Calmette-Guerin (BCG) vaccine developed a century ago, is effective against childhood (disseminated and miliary) TB. However, its protective efficacy against pulmonary TB varies from 0-80% in different populations. One of the main reasons for lack of an effective vaccine against TB is lack of complete understanding about correlates of protective immunity on which to base vaccine design and development. However, some household contacts who ar...
Source: Clinical and Developmental Immunology - December 14, 2020 Category: Allergy & Immunology Authors: Abebe F Tags: Clin Exp Immunol Source Type: research

Late diagnosis and advances in genetics of chronic granulomatous disease.
PMID: 33314034 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - December 13, 2020 Category: Allergy & Immunology Authors: Di Matteo G, Finocchi A Tags: Clin Exp Immunol Source Type: research

Pre-existing influenza specific nasal IgA or nasal viral infection does not affect live attenuated influenza vaccine immunogenicity in children.
In this study, we analysed banked samples from a study performed during the 2017/18 influenza season to investigate the role of pre-existing influenza-specific nasal IgA in children aged 6-14 years. Nasopharyngeal swabs were collected prior to LAIV immunisation, to measure pre-existing IgA levels and test for concurrent upper respiratory tract viral infections (URTI). Oral fluid samples were taken at baseline and 21-28 days after LAIV to measure IgG as a surrogate of immunogenicity. Antibody levels at baseline were compared with a pre-existing dataset of LAIV shedding from the same individuals, measured by RT-PCR. There wa...
Source: Clinical and Developmental Immunology - December 13, 2020 Category: Allergy & Immunology Authors: Cole ME, Kundu R, Abdulla AF, Andrews N, Hoschler K, Southern J, Jackson D, Miller E, Zambon M, Turner PJ, Tregoning JS Tags: Clin Exp Immunol Source Type: research

Vogt Koyanagi Harada patients show higher frequencies of circulating NKG2Dpos NK and NKT cells.
Abstract Vogt Koyanagi Harada (VKH) is an autoimmune disease characterized by inflammation in tissues that contain melanocytes. We aimed to increase the knowledge on immunological pathways deregulated in VKH disease. We compared the percentages of circulating NK, NKT and T cells expressing the activatory markers: CD16, CD69, NKG2D, Nkp30, Nkp46, and the inhibitory marker: NKG2A in 10 active VKH patients, 20 control subjects (CTR) and 7 patients with Behçet disease (BD) by flow cytometry. Cytotoxic potential of NK cells was determined through the degranulation marker CD107a expression after contact with K562...
Source: Clinical and Developmental Immunology - December 12, 2020 Category: Allergy & Immunology Authors: Bonacini M, Cimino L, De Simone L, Bolletta E, Gozzi F, Soriano A, Muratore F, Zerbini A, Fontana L, Salvarani C, Croci S Tags: Clin Exp Immunol Source Type: research

Influence of HLA-C environment on the spontaneous clearance of hepatitis C in European HIV-HCV co-infected individuals.
gne K Abstract Natural Killer (NK) cell functions are regulated by diverse inhibitory and activating receptors including Killer cell Immunoglobulin-like receptors (KIR) which interact with HLA class I molecules. Some KIR/HLA genetic combinations were reported associated with spontaneous clearance (SC) of hepatitis C virus (HCV) but with discordant results, possibly reflecting KIR and/or HLA gene polymorphism according to populations. KIR/HLA genetic combinations associated with both an exhaustive NK and T cell repertoire were investigated in a cohort of HIV-HCV co-infected individuals with either SC (n=68) or chro...
Source: Clinical and Developmental Immunology - December 12, 2020 Category: Allergy & Immunology Authors: Legrand N, David G, Rodallec A, Gaultier A, Salmon D, Cesbron A, Wittkop L, Raffi F, Gendzekhadze K, Retière C, Allavena C, Gagne K Tags: Clin Exp Immunol Source Type: research

Immune Checkpoint Expression on Peripheral Cytotoxic Lymphocytes in Cervical Cancer Patients: moving beyond the PD-1/PD-L1 axis.
We report expression of TIGIT, Tim-3, and PD-1 on subsets of peripheral blood NK (CD56dim/neg CD16bright/dim/neg and CD56bright CD16dim/neg ) and T cells. The percentages of these cells were increased in women with cervical cancer and premalignant lesions. PD-1+ NK and T cells were likely to co-express TIGIT and/or Tim-3. These cells, with an apparently "exhausted" phenotype, were augmented in patients. A subset of cells were also NKG2D and DNAM-1 positive. PD-1int and PD-1high T cells were notably increased in cervical cancer. Soluble PD-L1 was higher in cancer patient blood versus healthy donors and we observed...
Source: Clinical and Developmental Immunology - December 11, 2020 Category: Allergy & Immunology Authors: Solorzano-Ibarra F, Alejandre-Gonzalez AG, Ortiz-Lazareno PC, Bastidas-Ramirez BE, Zepeda-Moreno A, Tellez-Bañuelos MC, Banu N, Carrillo-Garibaldi OJ, Chavira-Alvarado A, Bueno-Topete MR, Del Toro-Arreola S, Haramati J Tags: Clin Exp Immunol Source Type: research

E3 Ubiquitin Ligase Cbl-b and its potential therapeutic implications for immunotherapy.
Abstract The distinction of self from non-self is crucial to prevent autoreactivity and assure protection from infectious agents and tumors. Maintaining the balance between immunity and tolerance of immune cells is strongly controlled by several sophisticated regulatory mechanisms of the immune system. Among them, the E3 ligase ubiquitin casitas B cell lymphoma-b (Cbl-b) is a newly identified component in the ubiquitin-dependent protein degradation system, which is thought to be an important negative regulator of immune cells. An update on the current knowledge and new concepts of the relevant immune homeostasis p...
Source: Clinical and Developmental Immunology - December 11, 2020 Category: Allergy & Immunology Authors: Jafari D, Mousavi MJ, Keshavarz Shahbaz S, Jafarzadeh L, Tahmasebi S, Spoor J, Esmaeilzadeh A Tags: Clin Exp Immunol Source Type: research

Cervical cytomegalovirus reactivation, cytokines, and spontaneous preterm birth in Kenyan women.
man D, Slyker J Abstract Genital cytomegalovirus (CMV) reactivation is common during the third trimester of pregnancy. We hypothesized cervical CMV shedding may increase risk of spontaneous preterm birth (sPTB) through the release of inflammatory cytokines in the cervix. We conducted a nested case-control analysis to determine the relationship between CMV shedding and sPTB using data and samples from a prospective cohort study in western Kenya. Women who delivered between 28+0 and 33+6 weeks gestation were matched by gestational age at sample collection to controls who delivered ≥37+0 weeks. Levels of CMV DNA a...
Source: Clinical and Developmental Immunology - December 3, 2020 Category: Allergy & Immunology Authors: Begnel ER, Drake AL, Kinuthia J, Matemo D, Huang ML, Ásbjörnsdóttir KH, Chohan V, Beima-Sofie K, John-Stewart G, Lehman D, Slyker J Tags: Clin Exp Immunol Source Type: research

Citrullinated inter-alpha-trypsin inhibitor heavy chain 4 in arthritic joints and its potential effect in the neutrophil migration.
CONCLUSION: Cit-ITIH4 is generated mainly in inflamed joints by neutrophils via PAD4. Citrullination of ITIH4 may change its function to upregulate neutrophilic migration by activating the complement cascade, exacerbating arthritis. PMID: 33238047 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - November 25, 2020 Category: Allergy & Immunology Authors: Osada A, Matsumoto I, Mikami N, Ohyama A, Kurata I, Kondo Y, Tsuboi H, Ishigami A, Sano Y, Arai T, Ise N, Sumida T Tags: Clin Exp Immunol Source Type: research

FAM96A knockout promotes alternative macrophage polarisation and protects mice against sepsis.
Abstract Sepsis is an intractable clinical syndrome characterised by organ dysfunction when the body over-responds to an infection. Sepsis has a high fatality rate and lacks effective treatment. Family with sequence similarity 96 member A (FAM96A) is an evolutionarily conserved protein with high expression in the immune system and is related to cytosolic iron-assembly and tumour suppression; however, research has been rarely conducted on its immune functions. Our study found that Fam96a-/- mice significantly resisted lesions during sepsis simulated by caecal ligation and puncture (CLP) or endotoxicosis models. Aft...
Source: Clinical and Developmental Immunology - November 24, 2020 Category: Allergy & Immunology Authors: Yin A, Chen W, Cao L, Li Q, Zhu X, Wang L Tags: Clin Exp Immunol Source Type: research

The Tellurium-based Immunomodulator, AS101 Ameliorates Adjuvant-Induced Arthritis in Rats.
Abstract BACKGROUND: Despite undeniable improvement in the management of rheumatoid arthritis (RA), the discovery of more effective, less toxic and, ideally, less immune suppressive drugs are much needed. In the current study, we set to explore the potential anti-rheumatic activity of the non-toxic, tellurium-based immunomodulator, AS101 in an experimental animal model of RA. METHODS: The effect of AS101 was assessed on Adjuvant-induced arthritis (AIA) rats. Clinical signs of arthritis were assessed. Histopathological examination was used to assess inflammation, synovial changes and tissue lesions. Very late ...
Source: Clinical and Developmental Immunology - November 18, 2020 Category: Allergy & Immunology Authors: Halpert G, Halperin Sheinfeld M, Monteran L, Sharif K, Volkov A, Nadler R, Schlesinger A, Barshak I, Kalechman Y, Blank M, Shoenfeld Y, Amital H Tags: Clin Exp Immunol Source Type: research

Expansion of Immature, Nucleated Red Blood Cells by Transient Low-Dose Methotrexate Immune Tolerance Induction in Mice.
This study demonstrated that TLD-MTX induced a transient reduction of mature RBCs in the blood and immature RBCs in the bone marrow followed by significant enrichment of immature, nucleated RBCs in the spleen and blood during the time of immune tolerance induction, which suggested modulation of erythropoiesis may be associated with the induction of immune tolerance to rhGAA. PMID: 33205401 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - November 18, 2020 Category: Allergy & Immunology Authors: Tran JQ, Grover D, Zhang M, Stapels M, Brennan R, Bangari DS, Piepenhagen PA, Roberts E, Oliva P, Zubair F, Vela JL, Richards SM, Joseph AM Tags: Clin Exp Immunol Source Type: research

A hyperacute immune map of ischaemic stroke patients reveals alterations to circulating innate and adaptive cells.
Abstract Systemic immune changes following ischaemic stroke are associated with increased susceptibility to infection and poor patient outcome due to their role in exacerbating the ischaemic injury and long-term disability. Alterations to the abundance or function of almost all components of the immune system post-stroke have been identified, including lymphocytes, monocytes and granulocytes. However, subsequent infections have often confounded the identification of stroke-specific effects. Global understanding of very early changes to systemic immunity is critical to identify immune targets to improve clinical ou...
Source: Clinical and Developmental Immunology - November 18, 2020 Category: Allergy & Immunology Authors: Krishnan S, O'Boyle C, Smith CJ, Hulme S, Allan SM, Grainger JR, Lawrence CB Tags: Clin Exp Immunol Source Type: research

Cytokine levels in children and adults with wheezing and asthma show specific patterns of variability over time.
oup Abstract Levels of cytokines are used for in-depth characterization of patients with asthma; however, the variability over time might be a critical confounder. To analyze the course of serum cytokines in children, adolescents and adults with asthma and in healthy controls and to propose statistical methods to control for seasonal effects. Out of n= 532 screened subjects, n= 514 (91.5%) were included in the All Age Asthma Cohort (ALLIANCE). The cohort included n= 279 children with either recurrent wheezing bronchitis (> 3 episodes) or doctor-diagnosed asthma, n= 75 healthy controls, n= 150 adult asthmatics a...
Source: Clinical and Developmental Immunology - November 17, 2020 Category: Allergy & Immunology Authors: Weckmann M, Thiele D, Liboschik L, Bahmer T, Pech M, Dittrich AM, Fuchs O, Happle C, Schaub B, Ricklefs I, Rabe K, Mutius EV, Hansen G, König IR, Kopp MV, ALLIANCE- study group Tags: Clin Exp Immunol Source Type: research

Does the Bacillus Calmette-Guerin vaccine have different protective effects on various strains of tuberculosis?
This study aimed to compare the effect of BCG vaccination history in the prevention of the occurrence of Mtb- Beijing and non-Beijing strains. METHODS: In this cross-sectional study, 64 patients with pulmonary tuberculosis (TB) were harvested from the Iranian border provinces (north-west and west). Isolates were subjected to restriction fragment length polymorphism (RFLP) analysis, using the Insertion Sequence IS6110 as a probe (IS6110 RFLP), and drug susceptibility testing by the proportion method. Samples were analyzed with Gel Compare II 6.6 and SPSS-18. RESULTS: The mean age (SD) of the patients was 54.4 (17....
Source: Clinical and Developmental Immunology - November 14, 2020 Category: Allergy & Immunology Authors: Kousha A, Farajnia S, Ansarin K, Khalili M, Shariat M, Sahebi L Tags: Clin Exp Immunol Source Type: research

Roles of cytokines and T cells in the pathogenesis of myasthenia gravis.
Abstract Myasthenia gravis (MG) is characterized by muscle weakness and fatigability caused by the presence of autoantibodies against the acetylcholine receptor (AChR) or the muscle-specific tyrosine kinase (MuSK). Activated T cells, B cells, and plasma cells, as well as cytokines, play important roles in the production of pathogenic autoantibodies and the induction of inflammation at the neuromuscular junction in MG. Many studies have focused on the role of cytokines and lymphocytes in anti-AChR antibody-positive MG. Chronic inflammation mediated by T helper 17 (Th17) cells, the promotion of autoantibody producti...
Source: Clinical and Developmental Immunology - November 13, 2020 Category: Allergy & Immunology Authors: Uzawa A, Kuwabara S, Suzuki S, Imai T, Murai H, Ozawa Y, Yasuda M, Nagane Y, Utsugisawa K Tags: Clin Exp Immunol Source Type: research

Lymphocyte subsets early predict mortality in a large series of hospitalized COVID-19 patients in Spain.
P, Gil-Herrera J Abstract BACKGROUND: The role of lymphocytes and their main subsets as prognostic factors of death in SARS-CoV-2-infected patients remains unclear, with no information obtained from patients outside China. We aimed to assess whether measuring lymphocyte subpopulations added clinical value to the total lymphocyte counting regarding mortality, when they were simultaneously tested at hospital admission. METHODS: Peripheral blood was analysed in 701 PCR-confirmed consecutive patients by lysed-no washed flow cytometry. Demographic and clinical features were registered in electronic medical record...
Source: Clinical and Developmental Immunology - November 13, 2020 Category: Allergy & Immunology Authors: Cantenys-Molina S, Fernández-Cruz E, Francos P, Lopez Bernaldo de Quirós JC, Muñoz P, Gil-Herrera J Tags: Clin Exp Immunol Source Type: research

Diagnostic characterization of respiratory allergies by means of a multiplex immunoassay.
Abstract Allergic sensitization is commonly assessed in patients by performing the skin prick test (SPT) or determining specific IgE levels in blood samples with the ImmunoCAPTM assay, which measures each allergen and sample separately. This paper explores the possibility to investigate respiratory allergies with a high throughput method, the Meso Scale Discovery (MSD) multiplex immunoassay, measuring IgE levels in low volumes of blood. The MSD multiplex immunoassay, developed and optimized with standards and allergens from Radim Diagnostics, was validated against the SPT and the ImmunoCAPTM assay. For 18 adults (...
Source: Clinical and Developmental Immunology - November 11, 2020 Category: Allergy & Immunology Authors: Millen JLM, Willems I, Slingers G, Raes M, Koppen G, Langie SAS Tags: Clin Exp Immunol Source Type: research

Isotype selection for antibody-based cancer therapy.
Abstract The clinical application of monoclonal antibodies (mAbs) revolutionised the field of cancer therapy as it enabled the successful treatment of previously untreatable types of cancer. Different mechanisms play a role in the anti-tumour effect of mAbs. These include blocking of tumour-specific growth factor receptors or of immune modulatory molecules as well as complement and cell-mediated tumour cell lysis. Thus, for many mAbs Fc-mediated effector functions critically contribute to the efficacy of treatment. As Ig isotypes differ in their ability to bind to FcRs on immune cells as well as in their ability t...
Source: Clinical and Developmental Immunology - November 5, 2020 Category: Allergy & Immunology Authors: Vukovic N, van Elsas A, Verbeek JS, Zaiss DMW Tags: Clin Exp Immunol Source Type: research

A year in our understanding of COVID-19.
PMID: 33125171 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - November 1, 2020 Category: Allergy & Immunology Authors: Thwaites RS Tags: Clin Exp Immunol Source Type: research

Revisiting B cell tolerance and autoantibodies in seropositive and seronegative autoimmune rheumatic disease (AIRD).
We describe how autoantibodies can directly contribute to AIRD pathogenesis through innate and adaptive immune mechanisms, eventually culminating in systemic inflammation and localized tissue damage. We conclude by discussing recent insights that suggest distinct AIRD have incorrectly been denominated seronegative. PMID: 33090496 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 22, 2020 Category: Allergy & Immunology Authors: Pouw JNJ, Leijten EFAE, van Laar JMJ, Boes MM Tags: Clin Exp Immunol Source Type: research

Lipid antigens in bile from patients with chronic liver diseases activate natural killer T cells.
Abstract Natural killer T (NKT) cells are an abundant subset of liver lymphocytes activated by lipid antigens presented on CD1d molecules that are expressed by cholangiocytes. We aimed to determine if bile from patients with chronic liver diseases contains antigenic lipids that can activate NKT cells. Using murine invariant (24.7, 24.8 and DN32.D3) and non-invariant (14S.6, 14S.7 and 14S.10) NKT hybridomas we investigated the presence of lipid antigens in bile collected from the gallbladder of patients undergoing liver transplantation due to end stage liver disease. Biliary microbiota profiles were generated using...
Source: Clinical and Developmental Immunology - October 21, 2020 Category: Allergy & Immunology Authors: Valestrand L, Lie Berntsen N, Zheng F, Schrumpf E, Hyll Hansen S, Hemming Karlsen T, Steven Blumberg R, Roksund Hov J, Jiang X, Melum E Tags: Clin Exp Immunol Source Type: research

Kinetic studies of galectin-10 release from eosinophils exposed to proliferating T cells.
CONCLUSION: Extracellular trap formation has mainly been associated with anti-bacterial defense, but we show a new putative function of these cellular formations, as mediators of T cell suppression by enabling the release of galectin-10 from eosinophils. PMID: 33080067 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 20, 2020 Category: Allergy & Immunology Authors: Lingblom C, Andersson K, Wennerås C Tags: Clin Exp Immunol Source Type: research

CD11c+ dendritic cells mediate antigen-specific suppression in extracorporeal photopheresis.
Abstract Extracorporeal photopheresis (ECP) represents one of the most widespread and effective cell therapies for graft-versus-host disease and other T cell-mediated disorders. However, the key factors affecting the therapeutic efficacy of ECP remain unclear. We hypothesized that therapeutic effects are mediated by ECP-treated antigen presenting dendritic cells (DC). To test this hypothesis, we used the experimental model of contact hypersensitivity (CHS). The ECP's therapeutic activity improved when the total cell dose of the ECP-treated cells was increased. We used different haptens during sensitization to demo...
Source: Clinical and Developmental Immunology - October 18, 2020 Category: Allergy & Immunology Authors: Hackstein H, Kalina A, Dorn B, Sofia Keil I, Baal N, Michel G, Brendel C, Neubauer A, Jakob T, Bein G Tags: Clin Exp Immunol Source Type: research

CD40L-stimulated B cells for  ex vivo expansion of polyspecific nonhuman primate regulatory T cells for translational studies.
CONCLUSIONS: We developed an approach for the expansion of highly suppressive MCM polyspecific Tregs through the use of a combination of CD40L-engeniered B cells with the potential to be translated to clinical studies. To our knowledge, this is the first report that uses a pool of MHC-mismatched CD40L-sBc to create polyspecific Tregs suitable for use in deceased-donor transplants. PMID: 33058141 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 15, 2020 Category: Allergy & Immunology Authors: Alonso-Guallart P, Llore N, Lopes E, Kofman S, Ho SH, Stern J, Pierre G, Bruestle K, Tang Q, Sykes M, Griesemer A Tags: Clin Exp Immunol Source Type: research

Whole Exome Sequencing of T-B+ Severe Combined Immunodeficiency Egyptian infants, JAK3 predominance and novel variants.
CONCLUSION: T-B+NK- SCID accounted for around 90% of the Egyptian patients with T-B+SCID. Of these T-B+NK- SCID cases, 60% were autosomal recessive syndromes caused by JAK3 mutations and 30% were X-linked syndromes. It might be useful to sequence the JAK3 gene (i.e. targeted Sanger sequencing) in all T-B+ SCID patients, especially after X-linked SCID has been ruled out. Hence, no more than 10% of T-B+ SCID patients might require next-generation for a molecular diagnosis. PMID: 33040328 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 10, 2020 Category: Allergy & Immunology Authors: El Hawary R, Meshaal S, Mauracher A, Opitz L, Abd Elaziz D, Lotfy S, Eldash A, Boutros J, Galal N, Pachlopnik Schmid J, Elmarsafy A Tags: Clin Exp Immunol Source Type: research

Interleukin-35 has a Tumor-promoting Role in Hepatocellular Carcinoma.
CONCLUSIONS: Elevated IL-35 had an immune suppressive role in HCC tumor microenvironments through affecting inhibitor receptor expression and cytokine secretion of CD4+ and CD8+ T cells. Dissection of the precise targets and underlying molecular mechanisms would mean alternative treatments for HCC patients. (250 words). PMID: 33030251 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 8, 2020 Category: Allergy & Immunology Authors: Liu X, Ren H, Guo H, Wang W, Zhao N Tags: Clin Exp Immunol Source Type: research

Identification of peripheral CD154+ T-cells and HLA-DRB1 as biomarkers of acute cellular rejection in adult liver transplant recipients.
This study aimed to analyse different surface antigens on T-cells in a cohort of adult liver patients undergoing LT to determine the influence on ACR using multiparametric flow cytometry functional assay. Thirty patients were monitored at baseline and during 1-year post-transplant. Two groups were established, with (ACR) and without (NACR) acute cellular rejection. Leukocyte, total lymphocyte, percentages of CD4+CD154+ and CD8+CD154+ T-cells, HLA mismatch between recipient-donor and their relation with ACR as well as the acute rejection frequencies were analysed. T-cells were stimulated with concanavalin A (Con-A) and surf...
Source: Clinical and Developmental Immunology - October 6, 2020 Category: Allergy & Immunology Authors: Boix F, Legaz I, Minhas A, Alfaro R, Jiménez-Coll V, Mrowiec A, Martínez-Banaclocha H, Galián JA, Botella C, Moya-Quiles MR, Sanchez-Bueno F, Robles R, de la Peña-Moral J, Ramirez P, Pons JA, Minguela A, Muro M Tags: Clin Exp Immunol Source Type: research

IVIG ameliorate inflammation in collagen induced arthritis- projection for IVIG therapy in rheumatoid arthritis.
Abstract Rheumatoid arthritis (RA) is a systemic inflammatory autoimmune disease that leads to joint destruction and disability. Despite a significant progress in administration of biological agents for RA patients, there is still a need for improved therapy. Intravenous-immunoglobulins (IVIG) , a pooled polyspecific immunoglobulin-G (IgG) extracted from 5,000-20,000 healthy subjects, showed beneficial therapeutic effect in patients with immune-deficiency, sepsis, and autoimmune diseases. The current study aim to investigate the beneficial effect of treatment with IVIG in established collagen induced arthritis in ...
Source: Clinical and Developmental Immunology - October 5, 2020 Category: Allergy & Immunology Authors: Halpert G, Katz I, Shovman O, Tarasov S, Ganina KK, Petrova N, Tocut M, Volkov A, Barshack I, Blank M, Amital H Tags: Clin Exp Immunol Source Type: research

Relapse rate and renal prognosis in ANCA-associated vasculitis according to long-term ANCA patterns.
CONCLUSION: Recurrent or persistent ANCA patterns are associated with a higher risk of clinical relapse. A recurrent ANCA pattern was associated with worsening renal function in anti-MPO-associated vasculitis. PMID: 33020895 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 4, 2020 Category: Allergy & Immunology Authors: Oristrell J, Loureiro-Amigo J, Solans R, Valenzuela MP, Monsálvez V, Segarra A, Amengual MJ, Marín A, Feijoo C, Tolosa C Tags: Clin Exp Immunol Source Type: research

Different Subsets of Haematopoietic Cells and Immune Cells in Bone Marrow Between Young and Old Donors.
Abstract Young donors are reported to be associated with better transplant outcomes than old donors in allogeneic hematopoietic stem cell transplantation (allo-HSCT), but the mechanism is still unclear. The current study compared the different subsets of haematopoietic stem cells (HSCs) and their progenitors as well as immune cells in bone marrow (BM) between young and old donors. The frequencies of HSCs, multipotent progenitors (MPPs) and myeloid progenitors, including common myeloid progenitors (CMPs) and megakaryocyte-erythroid progenitors (MEPs), were decreased, whereas those of lymphoid progenitors, including...
Source: Clinical and Developmental Immunology - October 4, 2020 Category: Allergy & Immunology Authors: Yao WL, Wen Q, Zhao HY, Tang SQ, Zhang YY, Wang Y, Xu LP, Zhang XH, Huang XJ, Kong Y Tags: Clin Exp Immunol Source Type: research

Correlations of the expression of γδ T cells and their costimulatory molecules TIGIT, PD-1, ICOS, and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with URSA.
Correlations of the expression of γδ T cells and their costimulatory molecules TIGIT, PD-1, ICOS, and BTLA with PR and PIBF in the peripheral blood and decidual tissues of women with URSA. Clin Exp Immunol. 2020 Oct 05;: Authors: Liang Q, Tong L, Xiang L, Shen S, Pan C, Liu C, Zhang H Abstract Semi-allogeneic embryos are not rejected by the maternal immune system due to maternal-fetal immune tolerance. Progesterone (P) receptor (PR)-expressing γδ T cells are present in healthy pregnant women. In the presence of P, these cells secrete an immunomodulatory protein called progester...
Source: Clinical and Developmental Immunology - October 4, 2020 Category: Allergy & Immunology Authors: Liang Q, Tong L, Xiang L, Shen S, Pan C, Liu C, Zhang H Tags: Clin Exp Immunol Source Type: research

Increased expression of CXCL2 in ACPA-positive rheumatoid arthritis and its role in osteoclastogenesis.
In conclusion, CXCL2 was highly expressed in ACPA+ RA than in ACPA- RA. CXCL2 promoted osteoclastogenesis and was related to bone erosion in RA, which suggest that the blockade of CXCL2 might be a novel strategy for the treatment of RA. PMID: 33010041 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 2, 2020 Category: Allergy & Immunology Authors: Wang X, Sun L, He N, An Z, Yu R, Li C, Li Y, Li Y, Liu X, Fang X, Zhao J Tags: Clin Exp Immunol Source Type: research

LILRA3 is increased in IBD patients and functions as an anti-inflammatory modulator.
We report for the first time that LILRA3 is related to IBD and functions as an anti-inflammatory modulator in U937 cells. PMID: 33006756 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - October 1, 2020 Category: Allergy & Immunology Authors: Lan X, Liu F, Ma J, Chang Y, Lan X, Xiang L, Shen X, Zhou F, Zhao Q Tags: Clin Exp Immunol Source Type: research

Inactivation of TMEM106A promotes lipopolysaccharide-induced inflammation via the MAPK and NF- κB signaling pathways in macrophages.
Inactivation of TMEM106A promotes lipopolysaccharide-induced inflammation via the MAPK and NF-κB signaling pathways in macrophages. Clin Exp Immunol. 2020 Oct 02;: Authors: Zhang X, Feng T, Zhou X, Sullivan PM, Hu F, Lou Y, Yu J, Feng J, Liu H, Chen Y Abstract Pattern recognition receptors, such as Toll-like receptors (TLRs) play an important role in host defense against invading microbial pathogens. Their activation must be precisely regulated, as inappropriate activation or overactivation of TLR signaling pathways may result in inflammatory disorders, such as septic shock or autoimmune disease...
Source: Clinical and Developmental Immunology - October 1, 2020 Category: Allergy & Immunology Authors: Zhang X, Feng T, Zhou X, Sullivan PM, Hu F, Lou Y, Yu J, Feng J, Liu H, Chen Y Tags: Clin Exp Immunol Source Type: research

Analysis of Scoring Systems for Primary Immunodeficiency Diagnosis in Adult Immunology Clinics.
CONCLUSION: A modified IDR score with seven additional criteria validated in adults referred to immunology with suspicion of a PID could be used clinically to aid PID diagnosis, although further validation in different patient cohorts is required before it is used in other contexts. PMID: 32990325 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - September 28, 2020 Category: Allergy & Immunology Authors: Toms K, Gkrania-Klotsas E, Kumararatne D Tags: Clin Exp Immunol Source Type: research

Type I Interferon regulates cytokine-delayed neutrophil apoptosis, reactive oxygen species production and chemokine expression.
Abstract Interferons (IFNs) are key regulators of a number of inflammatory conditions in which neutrophils play an important role in pathology, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE), where Type I IFNs are implicated in disease pathology. However, IFNs are usually generated in vivo together with other cytokines that also have immunoregulatory functions but such interactions are poorly-defined experimentally. We measured the effects of Type-I (IFNα) IFN, elevated in both RA and SLE, on the functions of healthy neutrophils incubated in vitro in the absence and presence of pro-...
Source: Clinical and Developmental Immunology - September 28, 2020 Category: Allergy & Immunology Authors: Glennon-Alty L, Moots RJ, Edwards SW, Wright HL Tags: Clin Exp Immunol Source Type: research

Innate immunity during SARS-CoV-2: evasion strategies and activation trigger hypoxia and vascular damage.
ker D Abstract Innate immune sensing of viral molecular patterns is essential for development of antiviral responses. Like many viruses, SARS-CoV-2 has evolved strategies to circumvent innate immune detection including low CpG levels in the genome, glycosylation to shield essential elements including the receptor binding domain, RNA shielding and generation of viral proteins that actively impede anti-viral interferon responses. Together these strategies allow widespread infection and increased viral load. Despite the efforts of immune subversion, SARS-CoV-2 infection activates innate immune pathways inducing ...
Source: Clinical and Developmental Immunology - September 25, 2020 Category: Allergy & Immunology Authors: Amor S, Fernández Blanco L, Baker D Tags: Clin Exp Immunol Source Type: research

Islet autoantibody profiles associated with higher diabetes risk in Lithuanian compared with English schoolchildren.
Abstract Over a 15 year period, the incidence of type 1 diabetes has doubled in Lithuania, whilst increasing by a third in England, however England still has the higher incidence. Analysis of sera collected from non-diabetic schoolchildren from Lithuania and England more than 20 years ago showed a similar number of multiple autoantibody positive schoolchildren between the populations, but a higher prevalence of islet antigen-2 autoantibodies (IA-2A) in English schoolchildren. We aimed to use recently developed, more specific islet autoantibody tests, to characterise differences in humoral autoimmunity between thes...
Source: Clinical and Developmental Immunology - September 25, 2020 Category: Allergy & Immunology Authors: Long AE, Caygill CH, Gillespie KM, Marčiulionytė D, Williams AJ Tags: Clin Exp Immunol Source Type: research

Relationship between natural and infection -induced antibodies in systemic autoimmune diseases (SAD); SLE, SSc and RA.
ák L, Németh P, Berki T Abstract BACKGROUND: Infection or vaccine-induced T cell-dependent immune response and the subsequent high affinity neutralizing antibody production have been extensively studied, while the connection between natural autoantibodies (nAAbs) and disease-specific antibodies has not been thoroughly investigated. Our goal was to find relationship between IgM and IgG isotype nAAbs and infection or vaccine-induced and disease-related autoantibody levels in systemic autoimmune diseases (SAD). METHODS: A previously described indirect ELISA test was used for detection of IgM/IgG nA...
Source: Clinical and Developmental Immunology - September 21, 2020 Category: Allergy & Immunology Authors: Böröcz K, Simon D, Erdő-Bonyár S, Kovács KT, Tuba É, Czirják L, Németh P, Berki T Tags: Clin Exp Immunol Source Type: research

"Defective Bcl-2 expression in memory B cells from CVID patients".
"Defective Bcl-2 expression in memory B cells from CVID patients". Clin Exp Immunol. 2020 Sep 22;: Authors: Del Pino Molina L, Torres Canizales JM, Pernía O, Rodríguez Pena R, Ibanez de Caceres I, López Granados E Abstract Common Variable Immunodeficiency (CVID) is a primary immunodeficiency characterized by hypogammaglobulinemia and different degrees of B-cell compartment alteration. Memory B-cell differentiation requires the orchestrated activation of several intracellular signalling pathways that lead to the activation of a number of factors, such as NFκB, which...
Source: Clinical and Developmental Immunology - September 21, 2020 Category: Allergy & Immunology Authors: Del Pino Molina L, Torres Canizales JM, Pernía O, Rodríguez Pena R, Ibanez de Caceres I, López Granados E Tags: Clin Exp Immunol Source Type: research

Clinical, functional and genetic characterization of Sixteen Patients Suffering from Chronic Granulomatous Disease variants - Identification of Eleven Novel Mutations in CYBB.
M, Gérard Michel, Bertrand Y, Plantaz D, Kelecic J, Traberg R, Kainulainen L, Fauré J, Fieschi F, Stasia MJ Abstract Chronic Granulomatous Disease (CGD) is a rare inherited disorder in which phagocytes lack NADPH oxidase activity. The most common form is the X-linked CGD (X91-CGD), caused by mutations in the CYBB gene. Clinical, functional and genetic characterizations of 16 CGD cases of male patients and their relatives were done. We classified them as suffering from different variants of CGD (X910 , X91- or X91+ ) according to NOX2 expression and NADPH oxidase activity in neutrophils. Eleven mutati...
Source: Clinical and Developmental Immunology - September 19, 2020 Category: Allergy & Immunology Authors: Mollin M, Beaumel S, Vigne B, Brault J, Roux-Buisson N, Rendu J, Barlogis V, Catho G, Dumeril C, Fouyssac F, Monnier D, Gandemer V, Revest M, Brion JP, Bost-Bru C, Jeziorski E, Laurence Eitenschenck, Jarrasse C, Haus SD, Houachée-Chardin M, Hancart M, G Tags: Clin Exp Immunol Source Type: research

Protease inhibitors elicit anti-inflammatory effects in CF mice with Pseudomonas aeruginosa acute lung infection.
CONCLUSIONS: Our results show that inhibition of protease activity elicits anti-inflammatory effects in CF mice with acute P. aeruginosa lung infection. Thus, Marimastat and Ilomastat represent candidate molecules for the treatment of CF patients, encouraging further studies on protease inhibitors and their application in inflammatory diseases. PMID: 32946591 [PubMed - as supplied by publisher] (Source: Clinical and Developmental Immunology)
Source: Clinical and Developmental Immunology - September 17, 2020 Category: Allergy & Immunology Authors: Sandri A, Lleo MM, Signoretto C, Boaretti M, Boschi F Tags: Clin Exp Immunol Source Type: research

The rise of complement in ANCA-associated vasculitis: from marginal player to target of modern therapy.
Abstract The complement system plays a central role in autoimmune diseases, including ANCA-associated vasculitis (AAV). Although complement deposition is scarce in AAV pathological samples, complement activation is required for development of necrotizing crescentic glomerulonephritis (NCGN) in mouse models of AAV and occurs via the alternative pathway. The anaphylatoxin C5a, produced by the final complement pathway, is determinant to drive the disease in animal models. C5a primes human neutrophils and enhances their activation induced by ANCA; activated neutrophils in turn release factors that lead to C5a generati...
Source: Clinical and Developmental Immunology - September 17, 2020 Category: Allergy & Immunology Authors: Trivioli G, Vaglio A Tags: Clin Exp Immunol Source Type: research