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Total 964 results found since Jan 2013.

Dexamethasone-conjugated polyethylenimine/MIF siRNA complex regulation of particulate matter-induced airway inflammation.
Abstract Inhalation of airborne particulate matter (PM), such as silicon dioxide (SiO2) and titanium dioxide (TiO2), induces acute lung inflammation. siRNA therapy has been proposed as a method to repair acute lung inflammation. To determine whether DEXA-PEI/MIF siRNA contributes to SiO2-induced acute lung inflammation repair, we administered Dexa-PEI/MIF siRNA in SiO2-treated Beas-2b cells and instilled DEXA-PEI-MIF siRNA intratracheally in mice with SiO2-induced acute lung inflammation. Using genetic (MIF mRNA RT-PCR), histological (H&E and PAS) and immunohistochemical (MIF and Muc5ac) analyses, we estimated...
Source: Biomaterials - July 4, 2013 Category: Materials Science Authors: Choi M, Lee M, Rhim T Tags: Biomaterials Source Type: research

Abstract B42: Silencing of DNA repair proteins with ECO/siRNA nanoparticles for the enhancement of radiation response in glioblastoma
In this study we investigate the use of these nanoparticles to deliver siRNA to inhibit ATM and DNApk activity and enhance radiation response in both glioma and glioma stem cell lines.Established glioma (U251) and glioma stem cell (NSC11) lines were used to evaluate the effectiveness of ECO nanoparticle delivery of siRNA in vitro . Cellular uptake of ECO nanoparticles loaded with fluorescent siRNA was assessed using flow cytometry and fluorescent microscopy, demonstrating the rapid uptake of ECO/siRNA nanoparticles in comparison to commercially available transfection agents. Protein and mRNA analyses revealed the kinetics ...
Source: Cancer Research - January 15, 2017 Category: Cancer & Oncology Authors: Jennifer A. Lee, Nadia Ayat, Anita Tandle, Zheng-Rong Lu, Kevin Camphausen Tags: Drug Delivery and Nanomedicine Source Type: research

Characterization and comparison of two novel nanosystems associated with siRNA for cellular therapy
In this study we developed and characterized two different siRNA NP. On one hand, sorbitan monooleate (Span®80) based NP incorporating the cationic components poly-l-arginine or cationized pullulan, thus allowing the association of siRNA were designed. These NP presented a small size (205nm) and a negative surface charge (−38mV). On the other hand, lipid nanocapsules (LNC) associating polymers with lipids and allowing encapsulation of siRNA complexed with lipoplexes were also developed. Their size was of 82nm with a positive surface charge of +7mV. Both NP could be frozen with appropriate cryoprotectors. Cytotoxicity an...
Source: International Journal of Pharmaceutics - December 13, 2015 Category: Drugs & Pharmacology Source Type: research

Small Players Ruling the Hard Game: siRNA in Bone Regeneration
This article is protected by copyright. All rights reserved
Source: Journal of Bone and Mineral Research - February 1, 2016 Category: Orthopaedics Authors: S Ghadakzadeh, M Mekhail, A Aoude, R Hamdy, M Tabrizian Tags: Review Source Type: research

Sustained delivery of siRNA poly- and lipopolyplexes from porous macromer-crosslinked gelatin gels
In this study, we systematically investigated cross-linked gelatin based hydrogel formulations (cGEL) as degradable controlled release matrices for siRNA. Aiming at the definition of correlations between cGEL composition, siRNA nanoparticle formulation, release kinetics of complexed siRNA and transfection efficiency, we combined five different cGEL formulations and three transfection systems, i.e. polyplexes with polyethyleneimine (PEI), PEI in combination with liposomes (lipopolyplexes) and polyplexes based on tyrosin-modified PEI (P10Y). It was found that the distribution of these poly-/lipopolyplexes, when applied onto ...
Source: International Journal of Pharmaceutics - May 9, 2017 Category: Drugs & Pharmacology Source Type: research

Splicing stimulates siRNA formation at < i > Drosophila < /i > DNA double-strand breaks
by Karin Merk, Marco Breinig, Romy B öttcher, Stefan Krebs, Helmut Blum, Michael Boutros, Klaus Förstemann DNA double-strand breaks trigger the production of locus-derived siRNAs in fruit flies, human cells and plants. At least in flies, their biogenesis depends on active transcription running towards the break. Since siRNAs derive from a double-stranded RNA precursor, a major question is how broken DN A ends can generate matching sense and antisense transcripts. We performed a genome-wide RNAi-screen in culturedDrosophila cells, which revealed that in addition to DNA repair factors, many spliceosome components are requ...
Source: PLoS Genetics - June 19, 2017 Category: Genetics & Stem Cells Authors: Karin Merk Source Type: research

Cancers, Vol. 12, Pages 3260: Improving Radiation Response in Glioblastoma Using ECO/siRNA Nanoparticles Targeting DNA Damage Repair
Camphausen Radiation therapy is a mainstay in the standard of care for glioblastoma (GBM), thus inhibiting the DNA damage response (DDR) is a major strategy to improve radiation response and therapeutic outcomes. Small interfering RNA (siRNA) therapy holds immeasurable potential for the treatment of GBM, however delivery of the siRNA payload remains the largest obstacle for clinical implementation. Here we demonstrate the effectiveness of the novel nanomaterial, ECO (1-aminoethylimino[bis(N-oleoylcysteinylaminoethyl) propionamide]), to deliver siRNA targeting DDR proteins ataxia telangiectasia mutated and DNA-dependen...
Source: Cancers - November 4, 2020 Category: Cancer & Oncology Authors: Jennifer A. Lee Nadia Ayat Zhanhu Sun Philip J. Tofilon Zheng-Rong Lu Kevin Camphausen Tags: Article Source Type: research

Development of modified siRNA molecules incorporating 5-fluoro-2'-deoxyuridine residues to enhance cytotoxicity
Therapeutic small interfering RNAs (siRNAs) are composed of chemically modified nucleotides, which enhance RNA stability and increase affinity in Watson–Crick base pairing. However, the precise fate of such modified nucleotides once the siRNA is degraded within the cell is unknown. Previously, we demonstrated that deoxythymidine release from degraded siRNAs reversed the cytotoxicity of thymidylate synthase (TS)-targeted siRNAs and other TS inhibitor compounds. We hypothesized that siRNAs could be designed with specific nucleoside analogues that, once released, would enhance siRNA cytotoxicity. TS-targeted siRNAs were...
Source: Nucleic Acids Research - April 22, 2013 Category: Research Authors: Wu, S.-y., Chen, T.-m., Gmeiner, W. H., Chu, E., Schmitz, J. C. Tags: RNA Source Type: research

Blockage of Src by Specific siRNA as a Novel Therapeutic Strategy to Prevent Destructive Repair in Steroid‐Associated Osteonecrosis in Rabbits
This study was designed to prove our hypothesis that blocking VEGF‐Src signaling pathway by specific Src siRNA was able to prevent destructive repair in a SAON rabbit model. Destructive repair in SAON was induced in rabbits. At 2, 4, 6 weeks after SAON induction, VEGF, anti‐VEGF, Src siRNA, Src siRNA + VEGF, control siRNA and saline were intramedullary injected into proximal femora for each group, respectively. Vascularization and permeability were quantified by dynamic contrast‐enhanced (DCE) MRI. At week 6 after SAON induction, proximal femora were dissected for micro‐CT‐based trabecular architecture with f...
Source: Journal of Bone and Mineral Research - April 27, 2015 Category: Orthopaedics Authors: Li‐zhen Zheng, Hui‐juan Cao, Shi‐hui Chen, Tao Tang, Wei‐min Fu, Le Huang, Dick Ho Kiu Chow, Yi‐xiang Wang, James Francis Griffith, Wei He, Hong Zhou, De‐wei Zhao, Ge Zhang, Xin‐luan Wang, Ling Qin Tags: Original Article Source Type: research

Abstract 1428: Targeting RRM2 by siRNA inhibits cellular invasion and represents a rational approach for inhibition of metastasis of head and neck and lung cancers
Background: Ribonucleotide reductase subunit M2 (RRM2) has been frequently observed to be aberrantly overexpressed in various tumors. RRM2 is a key enzyme, and essential regulator of balanced deoxyribonucleotides (dNTPs). It is critical for DNA replication and repair, and hence cell survival. In addition to DNA synthesis, it has been reported to modulate cellular invasiveness. However, the mechanisms through which RRM2 affects the invasive phenotype have not been elucidated.Methods: We evaluated the expression of RRM2 protein in metastatic (n = 40) and non-metastatic (n = 40) head and neck squamous cell carcinoma (HNSCC) t...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Rahman, M. A., Amin, A. R. M. R., Zhang, J., Nannapaneni, S., Saba, N. F., Chen, Z. G., Shin, D. M. Tags: Tumor Biology Source Type: research

An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome ‑protamine-DNA-siRNA complex with cell permeable peptides.
An enhanced RRM2 siRNA delivery to rheumatoid arthritis fibroblast-like synoviocytes through a liposome‑protamine-DNA-siRNA complex with cell permeable peptides. Int J Mol Med. 2018 Aug 08;: Authors: Wang X, Wang X, Sun J, Fu S Abstract Rheumatoid arthritis (RA) is considered to be a systemic autoimmune disease that induces systemic complications and progressive disability. It affects a large number of people. RA fibroblast‑like synoviocytes (RA‑FLS) promote the progression of RA through the secretion of proinflammatory cytokines and increasing invasiveness into the extracellular matrix. Therefo...
Source: International Journal of Molecular Medicine - August 8, 2018 Category: Molecular Biology Authors: Wang X, Wang X, Sun J, Fu S Tags: Int J Mol Med Source Type: research

Effect of siRNA-mediated silencing of p53R2 gene on sensitivity of T-ALL cellsto Daunorubicin
CONCLUSION: The results of the present study showed that silencing of p53R2 using siRNA can significantly increase the antitumor effects of Daunorubicin on T-ALL cells. Therefore, p53R2 siRNA has the potential to be used as an adjuvant therapy in combination with Daunorubicin in T-ALL.PMID:37419428 | DOI:10.1016/j.gene.2023.147622
Source: Gene - July 7, 2023 Category: Genetics & Stem Cells Authors: Omid KianiGhalesardi Farhad Zaker Abbas Ghotaslou Hassan Boustani Mohammad Reza Rezvani Jafar Kiani Minoo Shahidi Source Type: research

Preclinical evaluation of an O(6)-methylguanine-DNA methyltransferase-siRNA/liposome complex administered by convection-enhanced delivery to rat and porcine brains.
In this study, we aim to evaluate the safety of MGMT-siRNA/cationic liposome complexes and determine whether the convection-enhanced delivery of these complexes is suitable for clinical use by undertaking preclinical testing in laboratory animals. No significant adverse events were observed in rats receiving infusions of MGMT-siRNA/cationic liposome complex directly into the brain with or without TMZ administration. A pig which received the complex administered by CED also showed no evidence of neurological dysfunction or histological abnormalities. However, the complex did not appear to achieve effective distribution by C...
Source: American Journal of Translational Research - November 14, 2014 Category: Research Tags: Am J Transl Res Source Type: research

Influence on radiosensitivity of lung glandular cancer cells when ERCC1 gene silenced by targeted siRNA
Conclusions The radiation sensitivity of lung glandular tumor could be improved after the ERCC1 gene was silenced by siRNA.
Source: Asian Pacific Journal of Tropical Medicine - May 28, 2016 Category: Tropical Medicine Source Type: research