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Total 51 results found since Jan 2013.
STAT3 signaling mediates peritoneal fibrosis by activating hyperglycolysis
CONCLUSIONS: si-STAT3 attenuated the HG-induced EMT and hyperglycolysis, and the overexpression of STAT3 could induce EMT in HMrSV5 cells. 3PO could markedly attenuate HG-induced EMT by decreasing PFKEB3 in HMrSV5 cells. In addition, we demonstrated that inhibiting STAT3 signaling or peritoneal hyperglycolysis could attenuate peritoneal fibrosis and angiogenesis in vivo. Our findings linked the STAT3/PFKFB3 signaling to the development of PF. HG/STAT3/PFKFB3 might promote the progression of PF through regulating profibrosis and angiogenesis.PMID:36398234 | PMC:PMC9641446
Source: American Journal of Translational Research - November 18, 2022 Category: Research Authors: Jiao Fu Nan Li Mengmeng He Dake Huang Pei Zhang Source Type: research
Aptamer-conjugated nanoliposomes containing COL1A1 siRNA sensitize CRC cells to conventional chemotherapeutic drugs
In this study, As1411 aptamer-conjugated liposomes were used for targeted siRNA delivery against the COL1A1 gene in colorectal cancer (CRC) cells. Cationic liposomes were synthesized and siRNA loading and conjugation of aptamer were confirmed by gel shift assay and spectrophotometry method. Release of siRNA from liposomes was assessed using dialysis method. Binding and uptake of aptamer-conjugated liposomes to and into cancer cells was assessed by fluorescence microscopy and flowcytometry. Gene expression was evaluated using qRT-PCR. Cell viability, chemosensitivity and apoptosis were determined by MTT assay and Annexin/PI...
Source: Colloids and Surfaces - July 29, 2022 Category: Biotechnology Authors: Hamed Manoochehri Akram Jalali Hamid Tanzadehpanah Amir Taherkhani Rezvan Najafi Source Type: research
Evaluation of liver specific ionizable lipid nanocarrierin the delivery of siRNA
Chem Phys Lipids. 2022 May 24:105207. doi: 10.1016/j.chemphyslip.2022.105207. Online ahead of print.ABSTRACTHepcidin, a key regulator of iron homeostasis, has been implicated in the pathogenesis of various iron-related diseases. Although small interfering RNA (siRNA) are potent to modulate the expression of hepcidin, their bioavailability remains a major issue. The β-galactopyranoside-conjugated liposomes (GAL-liposome) targeting liver synthesized hepcidin were prepared by thin lipid film hydration method to encapsulate siRNA and the conjugation of β-galactopyranoside to the lipid nanocarrier was achieved by covalent che...
Source: Chemistry and Physics of Lipids - May 27, 2022 Category: Lipidology Authors: Shilpa Rana Archana Bhatnagar Suman Singh Nirmal Prabhakar Source Type: research
Astragalus Total Saponins Ameliorate Peritoneal Fibrosis by Promoting Mitochondrial Synthesis and Inhibiting Apoptosis
In conclusion, ATS treatment may be an effective therapeutic agent to inhibit high glucose-induced in peritoneal fibrosis through PGC-1[Formula: see text]-mediated apoptosis.PMID:34983328 | DOI:10.1142/S0192415X22500094
Source: The American Journal of Chinese Medicine - January 5, 2022 Category: Complementary Medicine Authors: Zheng-Hong Li Rong Xu Jun Shi Man-Shu Yu Yu Zhong Wei-Ming He Li-Ping Cao Mei-Xiao Sheng Source Type: research
Ischemia-Reperfusion Injury Reduces Kidney Folate Transporter Expression and Plasma Folate Levels
In conclusion, ischemia-reperfusion injury can downregulate Sp1 mediated-folate transporter expression in tubular cells, which may reduce folate reabsorption and lead to low folate status.
Source: Frontiers in Immunology - June 4, 2021 Category: Allergy & Immunology Source Type: research
Activation of RAS contributes to peritoneal fibrosis via dysregulation of low-density lipoprotein receptor.
In conclusion, increased intracellular RAS activity impaired lipid homeostasis and induced ECM accumulation in HPMCs by disrupting the LDLr pathway, which contributed to PF.
PMID: 33427062 [PubMed - as supplied by publisher]
Source: Am J Physiol Renal P... - January 11, 2021 Category: Urology & Nephrology Authors: Liu J, Feng Y, Li N, Shao QY, Zhang Q, Sun C, Xu P, Jiang CM Tags: Am J Physiol Renal Physiol Source Type: research
Elevated expression of HDAC6 in clinical peritoneal dialysis patients and its pathogenic role on peritoneal angiogenesis.
In this study, we analyzed the expression of HDAC6 in the peritoneum from patients with non-PD and PD-related peritonitis and dialysis effluent from stable PD patients. Our study revealed that HDAC6 expressed highly in the peritoneum with peritonitis and co-stained with α-smooth muscle actin (α-SMA), a biomarker of the myofibroblast. And the level of HDAC6 in the dialysate increased with time and positively correlated with transforming growth factor-β1 (TGF-β1), interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF), and negatively with cancer antigen 125 (CA125). In vitro, blockading HDAC6 with a selective...
Source: Renal Failure - September 1, 2020 Category: Urology & Nephrology Tags: Ren Fail Source Type: research
Co-culture of human fibroblasts, smooth muscle and endothelial cells promotes osteopontin induction in hypoxia.
This study aimed to determine if osteopontin (OPN) was induced in hypoxia and if OPN could be responsible for driving AVF failure. Identification of new factors that participate in remodelling of AVFs is a challenge. Three cell lines representing the cells of the three layers of the walls of arteries and veins, fibroblasts, smooth muscle cells and endothelial cells, were tested in mono- and co-culture in vitro for OPN expression and secretion in normoxia compared to hypoxia after silencing the hypoxia-inducible factors (HIF-1α, HIF-2α and HIF-1/2α) with siRNA or after treatment with an inhibitor of NF-kB. None of the ce...
Source: J Cell Mol Med - February 6, 2020 Category: Molecular Biology Authors: Sadaghianloo N, Contenti J, Dufies M, Parola J, Rouleau M, Lee S, Peyron JF, Fabbri L, Hassen-Khodja R, Pouysségur J, Bost F, Jean-Baptiste E, Dardik A, Mazure NM Tags: J Cell Mol Med Source Type: research
DDR1 and MT1-MMP Expression Levels Are Determinant for Triggering BIK-Mediated Apoptosis by 3D Type I Collagen Matrix in Invasive Basal-Like Breast Carcinoma Cells
In conclusion, and in agreement with the other studies (Ford et al., 2007; Koh et al., 2015; Toy et al., 2015; Takai et al., 2018), our data suggest that during the acquisition of mesenchymal features, the level of full-length DDR1 expression should be considered, in addition to the other markers, as an important biomarker in the prognosis of aggressive breast carcinomas. As summarized in Figure 9, the regulation of cell proliferation and apoptosis by the collagen/DDR1 axis involves a differential activation of DDR1 signaling pathway and thus BIK expression. In the case of the basal-like bre...
Source: Frontiers in Pharmacology - May 2, 2019 Category: Drugs & Pharmacology Source Type: research
Dimethylaminomicheliolide ameliorates peritoneal fibrosis through the activation of autophagy
This study aimed to investigate the protective effect of DMAMCL against PD-related PF and the mechanisms involved. In this study, we found that DMAMCL significantly decreased PD-induced extracellular matrix (ECM) deposition in a mouse model of PD, and that delayed DMAMCL administration halted the progression of PF in an established PD model. In addition, rapamycin administration induced autophagy and significantly ameliorated PF. The protective effect of DMAMCL against PF was weakened when co-administered with DMAMCL and 3-methyladenine. Inducing autophagy by rapamycin decreased transforming growth factor- β1–induced EC...
Source: Journal of Molecular Medicine - March 10, 2019 Category: Molecular Biology Source Type: research
Dimethylaminomicheliolide ameliorates peritoneal fibrosis through the activation of autophagy.
This study aimed to investigate the protective effect of DMAMCL against PD-related PF and the mechanisms involved. In this study, we found that DMAMCL significantly decreased PD-induced extracellular matrix (ECM) deposition in a mouse model of PD, and that delayed DMAMCL administration halted the progression of PF in an established PD model. In addition, rapamycin administration induced autophagy and significantly ameliorated PF. The protective effect of DMAMCL against PF was weakened when co-administered with DMAMCL and 3-methyladenine. Inducing autophagy by rapamycin decreased transforming growth factor-β1-induced ECM a...
Source: Molecular Medicine - March 10, 2019 Category: Molecular Biology Authors: Li S, Peng F, Gong W, Wu J, Wang Y, Xu Z, Liu W, Li H, Yin B, Zhang Y, Chen S, Luo C, Li P, Chen Y, Huang Q, Zhou W, Long H Tags: J Mol Med (Berl) Source Type: research
First identification of PODXL nonsense mutations in autosomal dominant focal segmental glomerulosclerosis
In this study, we report the identification of a heterozygous nonsense PODXL mutations (Arg326X) in a Chinese pedigree featured by proteinuria and renal insufficiency with AD inheritance by whole exome sequencing (WES). Total mRNA and PODXL protein abundance were decreased in available peripheral blood cell samples of two affected patients undergoing hemodialysis, compared to those in healthy controls and hemodialysis controls without PODXL mutation. We identified another novel PODXL heterozygous nonsense mutation (c.C1133G; p.Ser378X) in a British-Indian pedigree of AD-FSGS by WES. In vitro study showed that, human embryo...
Source: Clinical Science - December 6, 2018 Category: Biomedical Science Authors: Lin, F.-J., Yao, L., Hu, X.-Q., Bian, F., Ji, G., Jiang, G.-R., Gale, D. P., Ren, H.-Q. Tags: PublishAheadOfPrint Source Type: research
