Filtered By:
Nutrition: Potassium
This page shows you your search results in order of date. This is page number 3.
Order by Relevance | Date
Total 74 results found since Jan 2013.
KCNK13 potassium channels in the ventral tegmental area of rats are important for excitation of VTA neurons by ethanol
CONCLUSIONS: KCNK13 plays an important role in ethanol stimulation of rat VTA neurons, and KCNK13 is dynamically regulated by cell damage, by ethanol exposure, and during withdrawal. KCNK13 is a novel alcohol-sensitive protein and further investigation of this channel may offer new avenues for the development of agents useful in altering the rewarding effect of alcohol.PMID:33960499 | DOI:10.1111/acer.14630
Source: Alcoholism, Clinical and Experimental Research - May 7, 2021 Category: Addiction Authors: Chang You Bertha J Vandegrift Mark S Brodie Source Type: research
Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking.
This study was designed to identify the specific PKC isoform involved in the long-term regulation of I Ks current. The I Ks current was recorded using whole-cell patch-clamp technique in human embryonic kidney (HEK) 293B cell co-transfected with human KCNQ1/KCNE1 genes. The results revealed that both chronic activation of Ang II and PMA reduced the I Ks current in a long-term regulation (about 24 hours). Further evidence showed that PKCε knockdown by siRNA antagonized the AngII-induced chronic inhibition on the I Ks current, whereas knockdown of cPKC (PKCα and PKCβ) attenuated the inhibition effect of PMA on the curren...
Source: Channels - February 6, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research
The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration
BMB Rep. 2021 Jan 7:5184. Online ahead of print.ABSTRACTVoltage-gated potassium (Kv) channels are involved in many important cellular functions and play pivotal roles in cancer progression. The expression level of Kv2.1 was observed to be higher in the highly metastatic prostate cancer cells (PC-3), specifically in their membrane, than in immortalized prostate cells (WPMY-1 cells) and comparatively less metastatic prostate cancer cells (LNCaP and DU145 cells). However, Kv2.1 expression was significantly decreased when the cells were treated with anti-oxidants, such as N-acetylcysteine or ascorbic acid, implying that the hi...
Source: BMB Reports - January 7, 2021 Category: Biochemistry Authors: Hyun Woo Park Min Seok Song Hun Ju Sim Pan Dong Ryu So Yeong Lee Source Type: research
The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration.
Abstract
Voltage-gated potassium (Kv) channels are involved in many important cellular functions and play pivotal roles in cancer progression. The expression level of Kv2.1 was observed to be higher in the highly metastatic prostate cancer cells (PC-3), specifically in their membrane, than in immortalized prostate cells (WPMY-1 cells) and comparatively less metastatic prostate cancer cells (LNCaP and DU145 cells). However, Kv2.1 expression was significantly decreased when the cells were treated with anti-oxidants, such as N-acetylcysteine or ascorbic acid, implying that the highly expressed Kv2.1 could detect reac...
Source: BMB Reports - January 7, 2021 Category: Biochemistry Authors: Park HW, Song MS, Sim HJ, Ryu PD, Lee SY Tags: BMB Rep Source Type: research
AMPK upregulates KCa2.3 channels and ameliorates endothelial dysfunction in diet-induced obese mice.
Abstract
The opening of endothelial small-conductance calcium-activated potassium channels (KCa2.3) is essential for endothelium-dependent hyperpolarization (EDH), which predominantly occurs in small resistance arteries. Adenosine monophosphate-activated protein kinase (AMPK), an important metabolic regulator, has been implicated in regulating endothelial nitric oxide synthase activity. However, it was unclear whether AMPK regulated endothelial KCa2.3-mediated EDH-type vasodilation. Using bioinformatics analysis and myograph system, we investigated the regulation by AMPK of KCa2.3 in human umbilical vein endotheli...
Source: Biochemical Pharmacology - November 10, 2020 Category: Drugs & Pharmacology Authors: Pang ZD, Wang Y, Song Z, She G, Ma XZ, Sun X, Wu W, Lai BC, Zhang J, Zhang Y, Du XJ, Shyy JYJ, Deng XL Tags: Biochem Pharmacol Source Type: research
The antidiabetic drug glibenclamide exerts direct retinal neuroprotection.
Abstract
Sulfonylureas, widely used as hypoglycaemic agents in adults with type 2 diabetes, have neuroprotective effects in preclinical models of central nervous system injury, and in children with neuropsychomotor impairments linked to neonatal diabetes secondary to ATP-sensitive potassium channel mutations. In the human and rodent retina, we show that the glibenclamide activated channel SUR1 is expressed in the retina and enriched in the macula; we also show that it co-localizes with the potassium channel Kir6.2, and with the cation channel transporter TRPM4. Glibenclamide (glyburide), administered at doses that...
Source: Translational Research : the journal of laboratory and clinical medicine - October 17, 2020 Category: Laboratory Medicine Authors: Berdugo M, Delaunay K, Naud MC, Guegan J, Moulin A, Savoldelli M, Picard E, Radet L, Jonet L, Djerada Z, Gozalo C, Daruich A, Beltrand J, Jeanny JC, Kermorvant-Duchemin E, Crisanti P, Polak M, Behar-Cohen F Tags: Transl Res Source Type: research
Predisposition of Neonatal Maternal Separation to Visceral Hypersensitivity via Downregulation of Small-Conductance Calcium-Activated Potassium Channel Subtype 2 (SK2) in Mice.
Conclusions: ELS-induced visceral pain and visceral hypersensitivity are associated with the underfunction of SK2 channels in the spinal DH.
PMID: 33029124 [PubMed - in process]
Source: Neural Plasticity - October 10, 2020 Category: Neurology Authors: Wu K, Gao JH, Hua R, Peng XH, Wang H, Zhang YM Tags: Neural Plast Source Type: research
Characterization of a dual-action adulticidal and larvicidal interfering RNA pesticide targeting the < i > Shaker < /i > gene of multiple disease vector mosquitoes
by Keshava Mysore, Limb K. Hapairai, Longhua Sun, Ping Li, Chien-Wei Wang, Nicholas D. Scheel, Alexandra Lesnik, Jessica Igiede, Max P. Scheel, Na Wei, David W. Severson, Molly Duman-Scheel
The existing mosquito pesticide repertoire faces great challenges to sustainability, and new classes of pesticides are vitally needed to address established and emerging mosquito-borne infectious diseases. RNA interference- (RNAi-) based pesticides are emerging as a promising new biorational mosqu ito control strategy. In this investigation, we describe characterization of an interfering RNA pesticide (IRP) corresponding to the mosquit...
Source: PLoS Neglected Tropical Diseases - July 19, 2020 Category: Tropical Medicine Authors: Keshava Mysore Source Type: research
Epigenetic Restoration of Voltage ‐gated Potassium Channel Kv1.2 Alleviates Nerve Injury‐induced Neuropathic Pain
AbstractVoltage ‐gated potassium channels (Kv) are important regulators of neuronal excitability for its role of regulating resting membrane potential and repolarization. Recent studies show that Kv channels participate in neuropathic pain, but the detailed underlying mechanisms are far from being clear. In the c urrent study, we used siRNA, miR‐137 agomir and antagomir to regulate the expression of Kv1.2 in spinal cord and dorsal root ganglia (DRG) of naïve and chronic constriction injury (CCI) rats. Kv currents and neuron excitability in DRG neurons were examined by patch‐clamp whole‐cell recording to verify the...
Source: Journal of Neurochemistry - July 2, 2020 Category: Neuroscience Authors: Jingjing Zhang,
Lina Rong,
Jinping Shao,
Yidan Zhang,
Yaping Liu,
Sen Zhao,
Lei Li,
Wenli Yu,
Mengya Zhang,
Xiuhua Ren,
Qingzan Zhao,
Changlian Zhu,
Huan Luo,
Weidong Zang,
Jing Cao Tags: ORIGINAL ARTICLE Source Type: research
Stimulatory Role of SPAK Signaling in the Regulation of Large Conductance Ca2+-Activated Potassium (BK) Channel Protein Expression in Kidney
In this study, we investigated the effect of SPAK on renal BK protein expression in both HEK293 cells and mouse kidney. In HEK293 cells, siRNA-mediated knockdown of SPAK expression significantly reduced BK protein expression and increased ERK1/2 phosphorylation, whereas overexpression of SPAK significantly enhanced BK expression and decreased ERK1/2 phosphorylation in a dose-dependent manner. Knockdown of ERK1/2 prevented SPAK siRNA-mediated inhibition of BK expression. Similarly, pretreatment of HEK293 cells with either the lysosomal inhibitor bafilomycin A1 or the proteasomal inhibitor MG132 reversed the inhibitory effec...
Source: Frontiers in Physiology - July 1, 2020 Category: Physiology Source Type: research
NEK7 mediated assembly and activation of NLRP3 inflammasome downstream of potassium efflux in ventilator-induced lung injury.
In this study, we constructed an in vitro cyclic stretch (CS)-stimulated mouse lung epithelial (MLE-12) cell model that was transfected with NIMA-related kinase 7 (NEK7) small interfering RNA (siRNA) or scramble siRNA (sc siRNA) and pretreated with or without glibenclamide (glb). We also established a VILI mouse model, which was pretreated with glibenclamide or oridonin (Ori). Our goal was to investigate the regulatory effects of NEK7 on NLRP3 inflammasome activation and the anti-inflammatory effects of glibenclamide and oridonin on VILI. Mechanical stretch exaggerated the interaction between NEK7 and NLRP3, leading to ass...
Source: Biochemical Pharmacology - April 26, 2020 Category: Drugs & Pharmacology Authors: Liu H, Gu C, Liu M, Liu G, Wang Y Tags: Biochem Pharmacol Source Type: research
β-Arrestin2 regulates the rapid component of delayed rectifier K+ currents and cardiac action potential of guinea pig cardiomyocytes after adrenergic stimulation.
This study investigated the role of β-arrestin2 in the regulation of cardiac hERG/IKr potassium channel and AP during chronic adrenergic stimulation. Single left ventricular myocytes were isolated from guinea pig heart, and were transfected with adenovirus encoding β-arrestin2, or β-arrestin2 siRNA or an empty adenovirus. Cell cultures containing 10 nM isoproterenol, 1 nM phenylephrine or vehicle alone (control medium) were electro-physiologically examined after 48 h of incubation. Action potential duration at 50 and 90 % of repolarization (APD50 and APD90) were measured using whole-cell patch-clamp recording. Sustained...
Source: Cellular and Molecular Biology - December 29, 2019 Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research
LncRNA KCNQ1OT1 contributes to oxygen-glucose-deprivation/reoxygenation-induced injury via sponging miR-9 in cultured neurons to regulate MMP8.
Abstract
Our study proposed to investigate the function of potassium voltage-gated channel sub-family Q member 1 opposite strand 1 (KCNQ1OT1) in cerebral ischemia-reperfusion (I/R) injury and the underlying mechanism. We constructed an oxygen-glucose-deprivation/reoxygenation (OGD/R) model using the primary cortical neurons to mimic the cerebral I/R injury in vitro. Small inference RNA (siRNA) was used to silencing KCNQ1OT1. Dual luciferase assay was conducted to verify the interaction between KCNQ1OT1 and miR-9 and interaction between miR-9 and MMP8. CCK8 assay and flow cytometry analysis were applied for determi...
Source: Experimental and Molecular Pathology - December 10, 2019 Category: Pathology Authors: Ren Y, Gao XP, Liang H, Zhang H, Hu CY Tags: Exp Mol Pathol Source Type: research
MicroRNA 362-3p Reduces hERG-related Current and Inhibits Breast Cancer Cells Proliferation
Conclusion: miR-362-3p mediates the transcriptional regulation of hERG and is associated with survival in breast cancer. The potential for miR-362-3p to serve as a biomarker and inform therapeutic strategies warrants further investigation.
Source: Cancer Genomics and Proteomics - October 27, 2019 Category: Cancer & Oncology Authors: ASSIRI, A. A., MOURAD, N., SHAO, M., KIEL, P., LIU, W., SKAAR, T. C., OVERHOLSER, B. R. Tags: Article Source Type: research
AGEs impair Kv channel-mediated vasodilation of coronary arteries by activating the NF-κB signaling pathway in ZDF rats
Publication date: December 2019Source: Biomedicine & Pharmacotherapy, Volume 120Author(s): Qingbo Liu, Bing Hua, Wen Su, Beibing Di, Shandong Yu, Side Gao, Huirong Liu, Xueqiao Zhao, Weiping Li, Hongwei LiAbstractExcessive formation of advanced glycation end products (AGEs) impairs voltage-gated potassium (Kv) channels in rat coronary artery smooth muscle cells (CSMCs), resulting in weakened Kv-mediated coronary vasodilation. We hypothesized that induction of the nuclear factor-κB (NF-κB) signaling pathway by AGEs plays a significant role in the regulation of Kv channel-mediated vasodilation in Zucker diabetic fatty (ZDF...
Source: Biomedicine and Pharmacotherapy - October 18, 2019 Category: Drugs & Pharmacology Source Type: research
