Bibliometric analysis of nicotinic acetylcholine receptors channel research (2000-2020)
Channels (Austin). 2021 Dec;15(1):298-309. doi: 10.1080/19336950.2021.1882113.ABSTRACTTo explore the research status, hotspots, and trends in research on nicotinic acetylcholine receptor (nAChR) channel. The Web of Science core collection database from 2000 to 2020 was used as the data source. The visual analysis software VOSviewer1.6.16 and Citespace5.7 R3 were used to visualize the studies of the nAChR channel. The national/institutional distribution, journal distribution, authors, and related research were discussed. A total of 5,794 articles were obtained. The USA and the Utah System of Higher Education were the most p...
Source: Channels - February 22, 2021 Category: Molecular Biology Authors: Xueping Zhu Yan Zhou Guozhen Yuan Jingjing Shi Shuai Shi Limei Zhang Ruoning Chai Yihang Du Chenglin Duan Yuanhui Hu Source Type: research

Spinal AMPA receptors: Amenable players in central sensitization for chronic pain therapy?
Authors: Kopach O, Voitenko N Abstract The activity-dependent trafficking of AMPA receptors (AMPAR) mediates synaptic strength and plasticity, while the perturbed trafficking of the receptors of different subunit compositions has been linked to memory impairment and to causing neuropathology. In the spinal cord, nociceptive-induced changes in AMPAR trafficking determine the central sensitization of the dorsal horn (DH): changes in AMPAR subunit composition compromise the balance between synaptic excitation and inhibition, rendering interneurons hyperexcitable to afferent inputs, and promoting Ca2+ influx into the D...
Source: Channels - February 11, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking.
This study was designed to identify the specific PKC isoform involved in the long-term regulation of I Ks current. The I Ks current was recorded using whole-cell patch-clamp technique in human embryonic kidney (HEK) 293B cell co-transfected with human KCNQ1/KCNE1 genes. The results revealed that both chronic activation of Ang II and PMA reduced the I Ks current in a long-term regulation (about 24 hours). Further evidence showed that PKCε knockdown by siRNA antagonized the AngII-induced chronic inhibition on the I Ks current, whereas knockdown of cPKC (PKCα and PKCβ) attenuated the inhibition effect ...
Source: Channels - February 6, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Expression defect of the rare variant/Brugada mutation R1512W depends upon the SCN5A splice variant background and can be rescued by mexiletine and the common polymorphism H558R.
Conclusion : These findings provide further evidence that the splice variant affects the molecular phenotype with implications for the clinical phenotype, and they provide insight into the expression defect mechanisms and potential treatment in BrS. PMID: 33535892 [PubMed - in process] (Source: Channels)
Source: Channels - February 6, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Efficient expression of a cnidarian peptide-gated ion channel in mammalian cells.
Authors: Bachmann M, Ortega-Ramírez A, Leisle L, Gründer S Abstract Hydra Na+ channels (HyNaCs) are peptide-gated ion channels of the DEG/ENaC gene family that are directly activated by neuropeptides of the Hydra nervous system. They have previously been successfully characterized in Xenopus oocytes. To establish their expression in mammalian cells, we transiently expressed heteromeric HyNaC2/3/5 in human HEK 293 and monkey COS-7 cells. We found that expression of HyNaC2/3/5 using native cDNAs was inefficient and that codon optimization strongly increased protein expression and current amplitude in patc...
Source: Channels - February 3, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Differential expression of GluN2 NMDA receptor subunits in the dorsal horn of male and female rats.
Authors: Temi S, Rudyk C, Armstrong J, Landrigan JA, Dedek C, Salmaso N, Hildebrand ME Abstract N-methyl-D-aspartate receptors (NMDARs) are excitatory ionotropic glutamate receptors expressed throughout the CNS, including in the spinal dorsal horn. The GluN2 subtypes of NMDAR subunit, which include GluN2A, GluN2B, and GluN2D in the dorsal horn, confer NMDARs with structural and functional variability, enabling heterogeneity in synaptic transmission and plasticity. Despite essential roles for NMDARs in physiological and pathological pain processing, the distribution and function of these specific GluN2 isoforms acro...
Source: Channels - January 31, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Assessing the impact of pain-linked Nav1.7 variants: An example of two variants with no biophysical effect.
Authors: Le Cann K, Meents JE, Sudha Bhagavath Eswaran V, Dohrn MF, Bott R, Maier A, Bialer M, Hautvast P, Erickson A, Rolke R, Rothermel M, Körner J, Kurth I, Lampert A Abstract Mutations in the voltage-gated sodium channel Nav1.7 are linked to human pain. The Nav1.7/N1245S variant was described before in several patients suffering from primary erythromelalgia and/or olfactory hypersensitivity. We have identified this variant in a pain patient and a patient suffering from severe and life-threatening orthostatic hypotension. In addition, we report a female patient suffering from muscle pain and carrying the Na...
Source: Channels - January 27, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

TRPM2 in ischemic stroke: Structure, molecular mechanisms, and drug intervention.
Authors: Wang Q, Liu N, Ni YS, Yang JM, Ma L, Lan XB, Wu J, Niu JG, Yu JQ Abstract Ischemic stroke has a high lethality rate worldwide, and novel treatments are limited. Calcium overload is considered to be one of the mechanisms of cerebral ischemia. Transient receptor potential melastatin 2 (TRPM2) is a reactive oxygen species (ROS)-sensitive calcium channel. Cerebral ischemia-induced TRPM2 activation triggers abnormal intracellular Ca2+ accumulation and cell death, which in turn causes irreversible brain damage. Thus, TRPM2 has emerged as a new therapeutic target for ischemic stroke. This review provides data on ...
Source: Channels - January 21, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The focal adhesion protein Testin modulates KCNE2 potassium channel β subunit activity.
The focal adhesion protein Testin modulates KCNE2 potassium channel β subunit activity. Channels (Austin). 2021 Dec;15(1):229-238 Authors: Papanikolaou M, Crump SM, Abbott GW Abstract Coronary Artery Disease (CAD) typically kills more people globally each year than any other single cause of death. A better understanding of genetic predisposition to CAD and the underlying mechanisms will help to identify those most at risk and contribute to improved therapeutic approaches. KCNE2 is a functionally versatile, ubiquitously expressed potassium channel β subunit associated with CAD and cardiac arrh...
Source: Channels - January 21, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The resting membrane potential of hSC-CM in a syncytium is more hyperpolarised than that of isolated cells.
Authors: Van de Sande DV, Kopljar I, Maaike A, Teisman A, Gallacher DJ, Bart L, Snyders DJ, Leybaert L, Lu HR, Labro AJ Abstract Human-induced pluripotent stem cell (hiPSC) and stem cell (hSC) derived cardiomyocytes (CM) are gaining popularity as in vitro model for cardiology and pharmacology studies. A remaining flaw of these cells, as shown by single-cell electrophysiological characterization, is a more depolarized resting membrane potential (RMP) compared to native CM. Most reports attribute this to a lower expression of the Kir2.1 potassium channel that generates the IK1 current. However, most RMP recordings ar...
Source: Channels - January 21, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Changes in peripheral HCN2 channels during persistent inflammation.
Authors: Jansen LR, Forster LA, Smith XL, Rubaharan M, Murphy AZ, Baro DJ Abstract Nociceptor sensitization following nerve injury or inflammation leads to chronic pain. An increase in the nociceptor hyperpolarization-activated current, Ih, is observed in many models of pathological pain. Pharmacological blockade of Ih prevents the mechanical and thermal hypersensitivity that occurs during pathological pain. Alterations in the Hyperpolarization-activated Cyclic Nucleotide-gated ion channel 2 (HCN2) mediate Ih-dependent thermal and mechanical hyperalgesia. Limited knowledge exists regarding the nature of these chang...
Source: Channels - January 12, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Selective Targeting of Nav1.7 with Engineered Spider Venom-Based Peptides.
Authors: Neff RA, Wickenden AD Abstract A fundamental mechanism that drives the propagation of electrical signals in the nervous system is the activation of voltage-gated sodium channels. The sodium channel subtype Nav1.7 is critical for the transmission of pain-related signaling, with gain-of-function mutations in Nav1.7 resulting in various painful pathologies. Loss-of-function mutations cause complete insensitivity to pain and anosmia in humans that otherwise have normal nervous system function, rendering Nav1.7 an attractive target for the treatment of pain. Despite this, no Nav1.7 selective therapeutic has bee...
Source: Channels - January 12, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Comparison of quinazoline and benzoylpyrazoline chemotypes targeting the CaV α-β interaction as antagonists of the N-type CaV2.2 channel.
Comparison of quinazoline and benzoylpyrazoline chemotypes targeting the CaVα-β interaction as antagonists of the N-type CaV2.2 channel. Channels (Austin). 2021 Dec;15(1):128-135 Authors: Ran D, Gomez K, Moutal A, Patek M, Perez-Miller S, Khanna R Abstract Structural studies with an α subunit fragment of voltage-gated calcium (CaV) channels in complex with the CaVβ subunits revealed a high homology between the various CaVα-β subunits, predicting that targeting of this interface would result in nonselective compounds. Despite this likelihood, my laboratory initiated a ra...
Source: Channels - January 10, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Stabilization of negative activation voltages of Cav1.3 L-Type Ca2+-channels by alternative splicing.
Authors: Hofer NT, Pinggera A, Nikonishyna YV, Tuluc P, Fritz EM, Obermair GJ, Striessnig J Abstract -->Low voltage-activated Cav1.3 L-type Ca2+-channels are key regulators of neuronal excitability controlling neuronal development and different types of learning and memory. Their physiological functions are enabled by their negative activation voltage-range, which allows Cav1.3 to be active at subthreshold voltages. Alternative splicing in the C-terminus of their pore-forming α1-subunits gives rise to C-terminal long (Cav1.3L) and short (Cav1.3S) splice variants allowing Cav1.3S to activate at even mo...
Source: Channels - January 1, 2021 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Immunomagnetic separation is a suitable method for electrophysiology and ion channel pharmacology studies on T cells.
Authors: Tajti G, Szanto TG, Csoti A, Racz G, Evaristo C, Hajdu P, Panyi G Abstract Ion channels play pivotal role in the physiological and pathological function of immune cells. As immune cells represent a functionally diverse population, subtype-specific functional studies, such as single-cell electrophysiology require proper subset identification and separation. Magnetic-activated cell sorting (MACS) techniques provide an alternative to fluorescence-activated cell sorting (FACS), however, the potential impact of MACS-related beads on the biophysical and pharmacological properties of the ion channels were not stu...
Source: Channels - December 29, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Kv1.3 inhibition attenuates neuroinflammation through disruption of microglial calcium signaling.
Authors: Fomina AF, Nguyen HM, Wulff H Abstract In the last 5 years inhibitors of the potassium channel KV1.3 have been shown to reduce neuroinflammation in rodent models of ischemic stroke, Alzheimer's disease, Parkinson's disease and traumatic brain injury. At the systemic level these beneficial actions are mediated by a reduction in microglia activation and a suppression of pro-inflammatory cytokine and nitric oxide production. However, the molecular mechanisms for the suppressive action of KV1.3 blockers on pro-inflammatory microglia functions was not known until our group recently demonstrated that KV1.3 ...
Source: Channels - December 29, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The clinical and genetic heterogeneity analysis of five families with primary periodic paralysis.
Authors: Wang Q, Zhao Z, Shen H, Bing Q, Li N, Hu J Abstract To explore the clinical and genetic characteristics of five families with primary periodic paralysis (PPP). We reviewed clinical manifestations, laboratory results, electrocardiogram, electromyography, muscle biopsy, and genetic analysis from five families with PPP. Five families with PPP included: hypokalemic periodic paralysis type 1 (HypoPP1, CACNA1S, 1/5), hypokalemic periodic paralysis type 2 (HypoPP2, SCN4A, 2/5), normokalemic periodic paralysis (NormoPP, SCN4A, 1/5), and Andersen-Tawil syndrome (ATS, KCNJ2, 1/5). The basic clinical manifestations o...
Source: Channels - December 22, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Epigenetic control of ion channel expression and cell-specific splicing in nociceptors: Chronic pain mechanisms and potential therapeutic targets.
Authors: Lipscombe D, Lopez-Soto EJ Abstract Ion channels underlie all forms for electrical signaling including the transmission of information about harmful events. Voltage-gated calcium ion channels have dual function, they support electrical signaling as well as intracellular calcium signaling through excitation-dependent calcium entry across the plasma membrane. Mechanisms that regulate ion channel forms and actions are essential for myriad cell functions and these are targeted by drugs and therapeutics. When disrupted, the cellular mechanisms that control ion channel activity can contribute to disease pathophy...
Source: Channels - December 18, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Functional Modulation of the Human Voltage-Gated Sodium Channel NaV1.8 by Auxiliary β Subunits.
Functional Modulation of the Human Voltage-Gated Sodium Channel NaV1.8 by Auxiliary β Subunits. Channels (Austin). 2020 Dec 14;: Authors: Nevin ST, Lawrence N, Nicke A, Lewis RJ, Adams DJ Abstract The voltage-gated sodium channel Nav1.8 mediates the tetrodotoxin-resistant (TTX-R) Na+ current in nociceptive primary sensory neurons, which has an important role in the transmission of painful stimuli. Here, we describe the functional modulation of the human Nav1.8 α-subunit in Xenopus oocytes by auxiliary β subunits. We found that the β3 subunit down-regulated the maximal Na+ current a...
Source: Channels - December 16, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Trigeminal neuropathic pain is alleviated by inhibition of Cav3.3 T-type calcium channels in mice.
Authors: Montera M, Goins A, Cmarko L, Weiss N, Westlund High KN, Alles SRA Abstract In this brief report, we demonstrate that the Cav3.3 T-type voltage-gated calcium channel subtype is involved in our FRICT-ION model of chronic trigeminal neuropathic pain. We first showed that the Cacna1i gene encoding Cav3.3 is significantly upregulated in whole trigeminal ganglia of FRICT-ION mice compared to controls at week 10 post-injury. We confirmed protein upregulation of Cav3.3 compared to controls using Western blot analysis of whole trigeminal ganglia tissues. Finally, we demonstrated that intraperitoneal injection of a...
Source: Channels - December 9, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Late sodium current and calcium homeostasis in arrhythmogenesis.
Authors: Kistamás K, Hézső T, Horváth B, Nánási PP Abstract The cardiac late sodium current (INa,late) is the small sustained component of the sodium current active during the plateau phase of the action potential. Several studies demonstrated that augmentation of the current can lead to cardiac arrhythmias, therefore INa,late is considered as a promising antiarrhythmic target. Fundamentally, enlarged INa,late increases Na+ influx into the cell, which, in turn, is converted to elevated intracellular Ca2+ concentration through the Na+/Ca2+ exchanger. The excessive Ca2+ load is kno...
Source: Channels - December 2, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Acid-Sensing Ion Channel 3: An Analgesic Target.
Authors: Dulai JS, Smith ESJ, Rahman T Abstract Acid-sensing ion channel 3 (ASIC3) belongs to the epithelial sodium channel/degenerin (ENaC/DEG) superfamily. It consists of one of 7 different ASIC subunits encoded by 5 different genes. Most ASIC subunits form trimeric ion channels that upon activation by extracellular protons mediate a transient inward current inducing cellular excitability. ASIC subunits exhibit differential tissue expression and biophysical properties, and the ability of subunits to form homo- and heteromeric trimers further increases the complexity of currents measured and their pharmacological ...
Source: Channels - December 2, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The cool things to know about TRPM8!
Authors: Iftinca M, Altier C Abstract Transient receptor potential melastatin 8 (TRPM8) channels play a central role in the detection of environmental cold temperatures in the somatosensory system. TRPM8 is found in a subset of unmyelinated (C-type) afferents located in the dorsal root (DRG) and trigeminal ganglion (TG). Cold hypersensitivity is a common symptom of neuropathic pain conditions caused by cancer therapy, spinal cord injury, viral infection, multiple sclerosis, diabetes, or withdrawal symptoms associated with chronic morphine treatment. Therefore, TRPM8 has received great attention as a therapeutic tar...
Source: Channels - November 6, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Bibliometric analysis of chloride channel research (2004-2019).
Authors: Shi J, Shi S, Yuan G, Jia Q, Shi S, Zhu X, Zhou Y, Chen T, Hu Y PMID: 33103563 [PubMed - in process] (Source: Channels)
Source: Channels - October 27, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Similarities between the effect of SARS-CoV-2 and HCV on the cellular level, and the possible role of ion channels in COVID19 progression: a review of potential targets for diagnosis and treatment.
Authors: Alothaid H, Aldughaim MSK, El Bakkouri K, AlMashhadi S, Al-Qahtani AA Abstract The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has prompted an urgent need to identify effective medicines for the prevention and treatment of the disease. A comparative analysis between SARS-CoV-2 and Hepatitis C Virus (HCV) can expand the available knowledge regarding the virology and potential drug targets against these viruses. Interestingly, comparing HCV with SARS-CoV-2 reveals major similarities between them, ranging from the ion channels that are utilized, to the symptoms t...
Source: Channels - October 25, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Cav2.3 channel function and Zn2+-induced modulation: potential mechanisms and (patho)physiological relevance.
Authors: Neumaier F, Schneider T, Albanna W Abstract Voltage-gated calcium channels (VGCCs) are critical for Ca2+ influx into all types of excitable cells, but their exact function is still poorly understood. Recent reconstruction of homology models for all human VGCCs at atomic resolution provides the opportunity for a structure-based discussion of VGCC function and novel insights into the mechanisms underlying Ca2+ selective flux through these channels. In the present review, we use these data as a basis to examine the structure, function, and Zn2+-induced modulation of Cav2.3 VGCCs, which mediate native R-type c...
Source: Channels - October 22, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Experimental induction and mathematical modeling of Ca 2+ dynamics in rat round spermatids.
Authors: Saavedra J, Reyes JG, Salinas DG Abstract Cytosolic Ca2+ concentration ([Ca2+ ]) has an important role in spermatozoa and hence it regulates fertilization. In male germinal cells, there are indirect evidences that this ion could regulate physiological processes in spermatogenesis. Since little is known about Ca 2+ homeostasis in spermatogenic cells, in this work we propose a mathematical model that accounts for experimental [Ca2+ ] dynamics triggered by blockade of the SERCA transport ATPase with thapsigargin in round rat spermatids, without external Ca2+ and with different extracellular lactate concentrat...
Source: Channels - October 10, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Analysis of Arabidopsis TPK2 and KCO3 reveals structural properties required for K+ channel function.
Authors: Uehara C, Takeda K, Ibuki T, Furuta T, Hoshi N, Tanudjaja E, Uozumi N Abstract Arabidopsis thaliana contains five tandem-pore domain potassium channels, TPK1-TPK5 and the related one-pore domain potassium channel, KCO3. Although KCO3 is unlikely to be an active channel, it still has a physiological role in plant cells. TPK2 is most similar to KCO3 and both are localized to the tonoplast. However, their function remains poorly understood. Here, taking advantage of the similarities between TPK2 and KCO3, we evaluated Ca2+ binding to the EF hands in TPK2, and the elements of KCO3 required for K+ channel activ...
Source: Channels - October 7, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Modulation of voltage-gated CaV2.2 Ca2+ channels by newly identified interaction partners.
Authors: Lacinova L, Mallmann RT, Jurkovičová-Tarabová B, Klugbauer N Abstract Voltage-gated Ca2+ channels are typically integrated in a complex network of protein-protein-interactions, also referred to as Ca2+ channel nanodomains. Amongst the neuronal CaV2 channel family, CaV2.2 is of particular importance due to its general role for signal transmission from the periphery to the central nervous system, but also due to its significance for pain perception. Thus, CaV2.2 is an ideal target candidate to search for pharmacological inhibitors but also for novel modulatory interactors. In this review we su...
Source: Channels - October 4, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Modulation of Ca2+-induced Ca2+ release by ubiquitin protein ligase E3 component n-recognin UBR3 and 6 in cardiac myocytes.
Authors: Ma XE, Liu B, Zhao CX Abstract Ca2+-induced Ca2+ release (CICR) from sarcoplasmic reticulum is a finely tuned process responsible for cardiac excitation and contraction. The ubiquitin-proteasome system (UPS) as a major degradative system plays a crucial role in the maintenance of Ca2+ homeostasis. The E3 component N-recognin (UBR) subfamily is a part of the UPS; however, the role of UBR in regulating cardiac CICR is unknown. In the present study, we found that among the UBR family, single knockdown of UBR3 or UBR6 significantly elevated the amplitude of sarcoplasmic reticulum Ca2+ release without affecting...
Source: Channels - September 30, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Charged pore-lining residues are required for normal channel kinetics in the eukaryotic mechanosensitive ion channel MSL1.
Authors: Schlegel AM, Haswell ES Abstract Mechanosensitive (MS) ion channels are widespread mechanisms for cellular mechanosensation that can be directly activated by increasing membrane tension. The well-studied MscS family of MS ion channels is found in bacteria, archaea, and plants. MscS-Like (MSL)1 is localized to the inner mitochondrial membrane of Arabidopsis thaliana, where it is required for normal mitochondrial responses to oxidative stress. Like Escherichia coli MscS, MSL1 has a pore-lining helix that is kinked. However, in MSL1 this kink is comprised of two charged pore-lining residues, R326 and D327. Us...
Source: Channels - September 30, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Gating and regulation of KCNH (ERG, EAG, and ELK) channels by intracellular domains.
Authors: Codding SJ, Johnson AA, Trudeau MC Abstract The KCNH family comprises the ERG, EAG, and ELK voltage-activated, potassium-selective channels. Distinct from other K channels, KCNH channels contain unique structural domains, including a PAS (Per-Arnt-Sim) domain in the N-terminal region and a CNBHD (cyclic nucleotide-binding homology domain) in the C-terminal region. The intracellular PAS domains and CNBHDs interact directly and regulate some of the characteristic gating properties of each type of KCNH channel. The PAS-CNBHD interaction regulates slow closing (deactivation) of hERG channels, the kinetics of a...
Source: Channels - September 16, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Calmodulin binds to the N-terminal domain of the cardiac sodium channel Nav1.5.
In conclusion, we describe the new natural BrS Nav1.5 variant Y87C and present first evidence that calmodulin binds to the Nav1.5 NTD, which seems to be a determinant for the DNE. PMID: 32815768 [PubMed - in process] (Source: Channels)
Source: Channels - August 22, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Loss of Cav1.2 channels impairs hippocampal theta burst stimulation-induced long-term potentiation.
Authors: Sridharan PS, Lu Y, Rice RC, Pieper AA, Rajadhyaksha AM Abstract CACNA1 C, which codes for the Cav1.2 isoform of L-type Ca2+ channels (LTCCs), is a prominent risk gene in neuropsychiatric and neurodegenerative conditions. A role forLTCCs, and Cav1.2 in particular, in transcription-dependent late long-term potentiation (LTP) has long been known. Here, we report that elimination of Cav1.2 channels in glutamatergic neurons also impairs theta burst stimulation (TBS)-induced LTP in the hippocampus, known to be transcription-independent and dependent on N-methyl D-aspartate receptors (NMDARs) and local prot...
Source: Channels - August 18, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Polymodal roles of TRPC3 channel in the kidney.
Authors: Khayyat NH, Tomilin VN, Zaika O, Pochynyuk O Abstract TRPC3 is a Ca2+-permeable cation channel commonly activated by the G-protein coupled receptors (GPCR) and mechanical distortion of the plasma membrane. TRPC3-mediated Ca2+ influx has been implicated in a variety of signaling processes in both excitable and non-excitable cells. Kidneys play a commanding role in maintaining whole-body homeostasis and setting blood pressure. TRPC3 is expressed abundantly in the renal vasculature and in epithelial cells, where it is well positioned to mediate signaling and transport functions in response to GPCR-dependent e...
Source: Channels - August 15, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Gap junctions amplify TRPV4 activation-initiated cell injury via modification of intracellular Ca2+ and Ca2+-dependent regulation of TXNIP.
Authors: Zhang X, Mao Z, Huang Y, Zhang Z, Yao J Abstract The elevated intracellular Ca2+ and oxidative stress are well-reported mechanisms behind renal tubular epithelial injury initiated by various insults. Given that TRPV4 and connexin43 (Cx43) channels are activated by a wide range of stimuli and regulate both intracellular Ca2+ and redox status, we speculated an involvement of these channels in renal tubular cell injury. Here, we tested this possibility and explored the potential underlying mechanisms. Our results demonstrated that exposure of renal tubular epithelial cells to aminoglycoside G418 led to cell d...
Source: Channels - August 12, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

A low voltage activated Ca2+ current found in a subset of human ventricular myocytes.
In conclusion, ICa-LVA was detected in some HLVMs of a small portion of human hearts that happened to be nonischemic failing hearts. PMID: 32684070 [PubMed - in process] (Source: Channels)
Source: Channels - July 22, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Bibliometric analysis of calcium channel research (2010-2019).
Authors: Shi J, Wang H, Shi S, Yuan G, Jia Q, Shi S, Zhang X, Hu Y Abstract Calcium channels are involved in pathologies across all the major therapeutic areas involving the cardiac, neurological, metabolic, and respiratory systems. Although calcium channels have been the hotspot of multidisciplinary research for decades, the hotspots and frontier trends of calcium channel research have not been comprehensively analyzed by bibliometrics. Here, we collected scientific publications on calcium channel research in the past decade to explore the hotspots and frontier directions of calcium channel research by bibliometri...
Source: Channels - July 15, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

A bibliometric analysis and review of recent researches on TRPM7.
In this report, a total of 860 publications related to TRPM7 were analyzed. Biophysical Journal ranked top for publishing 31 papers. The United States of America had the largest number of publications (320) with a high citation frequency (11,298) and H-index (58). Chubanov V (38 publications) and Gudermann T (38 citations), who from Ludwig Maximilian University of Munich, were the most productive authors and had the greatest co-citation counts. Our study also combined the bibliometric study with a systematic review on TRPM7, highlighting the four research frontiers of TRPM7. This is the first study that demonstrated the tr...
Source: Channels - July 11, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

Sphingosine Kinase 2 Inhibitor ABC294640 Suppresses Neuronal Excitability and Inhibits Multiple Endogenously and Exogenously Expressed Voltage-Gated Ion Channels in Cultured Cells.
Authors: Zhang F, Hu W, Qu L, Cang C Abstract Sphingolipidsregulatemultiplecellularprocesses, including proliferation,autophagy, andapoptosis.Sphingosinekinases,thekeyenzymesinthemetabolismofsphingolipids,areoverexpressedinmanycancers,makingthemimportanttargetsforthedevelopmentof antitumordrugs.ABC294640isaselectivesphingosinekinase2(SK2)inhibitorthatshowsgoodantitumoractivityinvitro. One phaseIclinicalstudyofABC294640reportedthatABC294640causedavarietyofneurologicaldisorders. The mechanismofthesephenomena, however, remainsunclear.Inthepresentstudy,weusedinvitrocellexperimentstotesttheeffectsofABC294640onthenervous...
Source: Channels - July 4, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The Slo3/Lrrc52 complex is sensitive to phosphoinositides.
Authors: Kawai T, Okamura Y Abstract The voltage-sensing phosphatase (VSP) is a unique protein that shows voltage-dependent phosphatase activity toward phosphoinositides. Recently, we reported that VSP is activated and generates polarized PtdIns(4,5)P2 distribution in sperm flagellum. Interestingly, such specialized PtdIns(4,5)P2 distribution appears to contribute to the activity of Slo3, a sperm-specific K+ channels. It has been already reported that Slo3 activity is upregulated by PtdIns(4,5)P2 using a heterologous expression system. However, PtdIns(4,5)P2-dependence of Slo3 activity has not been studied in heter...
Source: Channels - June 24, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The effect of MD1 on potassium and L-type calcium current of cardiomyocytes from high-fat diet mice.
Authors: Shuai W, Kong B, Fu H, Jiang X, Huang H Abstract Myeloid differentiation protein 1 (MD1) is exerted an anti-arrhythmic effect in obese mice. Therefore, we sought to clarify whether MD1 can alter the electrophysiological remodeling of cardiac myocytes from obese mice by regulating voltage-gated potassium current and calcium current. MD1 knock-out (KO) and wild type (WT) mice were given a high-fat diet (HFD) for 20 weeks, starting at the age of 6 weeks. The potential electrophysiological mechanisms were estimated by whole-cell patch-clamp and molecular analysis. After 20-week HFD feeding, action po...
Source: Channels - June 5, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

A de novo cacna1d missense mutation in a patient with congenital hyperinsulinism, primary hyperaldosteronism and hypotonia.
A DE NOVO CACNA1D MISSENSE MUTATION IN A PATIENT WITH CONGENITAL HYPERINSULINISM, PRIMARY HYPERALDOSTERONISM AND HYPOTONIA. Channels (Austin). 2020 Apr 27;: Authors: De Mingo Alemany MC, Mifsud Grau L, Moreno Macián F, Ferrer Lorente B, León Cariñena S Abstract Congenital hyperinsulinemic hypoglycemia is the most frequent cause of persistent and recurrent hypoglycemia in the first years of life and in many patients rare genetic variants can be identified. Recently a case of congenital hyperinsulinemic hypoglycemia and a severe neurodevelopmental syndrome due to a mutation in the vo...
Source: Channels - April 29, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The CaMKII phosphorylation site Thr1604 in the CaV1.2 channel is involved in pathological myocardial hypertrophy in rats.
Authors: Li J, Wang S, Zhang J, Liu Y, Zheng X, Ding F, Sun X, Zhao M, Hao L Abstract Residue Thr1604 in the CaV1.2 channel is a Ca2+/calmodulin dependent protein kinase II (CaMKII) phosphorylation site, and its phosphorylation status maintains the basic activity of the channel. However, the role of CaV1.2 phosphorylation at Thr1604 in myocardial hypertrophy is incompletely understood. Isoproterenol (ISO) was used to induce cardiomyocyte hypertrophy, and autocamtide-2-related inhibitory peptide (AIP) was added as a treatment. Rats in a myocardial hypertrophy development model were subcutaneously injected with ISO f...
Source: Channels - April 16, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research

The mutation L69P in the PAS domain of the hERG potassium channel results in LQTS by trafficking deficiency.
Authors: Jenewein T, Kanner SA, Bauer D, Hertel B, Colecraft HM, Moroni A, Thiel G, Kauferstein S Abstract The congenital long QT syndrome (LQTS) is a cardiac disorder characterized by a prolonged QT interval on the electrocardiogram and an increased susceptibility to ventricular arrhythmias and sudden cardiac death. A frequent cause for LQTS are mutations in the KCNH2 gene (also known as the human ether-a-go-go-related gene or hERG), which reduce or modulate the potassium current IKr and hence alter cardiac repolarization. In a patient with a clinically diagnosed LQTS we identified the mutation L69P in the N-termi...
Source: Channels - April 9, 2020 Category: Molecular Biology Tags: Channels (Austin) Source Type: research