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Total 107 results found since Jan 2013.
Effects and regulatory pathway of proopinmelanocortin on feeding habit domestication in mandarin fish
Gene. 2023 Jun 17:147581. doi: 10.1016/j.gene.2023.147581. Online ahead of print.ABSTRACTProopiomelanocortin (POMC) is a hormone precursor, and has been reported to participate in domestication. However, its effects on feeding habit domestication in fish are poorly understood. Mandarin fish (Siniperca chuatsi) feeds solely on live prey fish since first-feeding. In the present study, the high expression of pomc in mandarin fish, both the pomc siRNA and MC4R inhibitor treatments increased the success rate of domestication from live prey fish to dead prey fish and food intake of dead prey fish, suggesting the role of pomc on ...
Source: Gene - June 19, 2023 Category: Genetics & Stem Cells Authors: Hai-Lin Lu Ling Li Yun-Liang Miao Hui Liang Jia-Ming Zou Jun-Jie You Xu-Fang Liang Shan He Source Type: research
Molecular Characterization of U6 Promoters from Orange-Spotted Grouper (Epinephelus coioides) and Its Application in DNA Vector-Based RNAi Technology
Mar Biotechnol (NY). 2023 May 8. doi: 10.1007/s10126-023-10212-9. Online ahead of print.ABSTRACTThe U6 promoter, a typical RNA polymerase III promoter, is widely used to transcribe small RNAs in vector-based siRNA systems. The RNAi efficiency is mainly dependent on the transcriptional activity of the U6 promoter. However, studies have found that U6 promoters isolated from some fishes do not work well in distantly related species. To isolate a U6 promoter with high transcriptional efficiency from fish, in this study, we cloned five U6 promoters in orange-spotted grouper, of which only the grouper U6-1 (GU6-1) promoter conta...
Source: Marine Biotechnology - May 8, 2023 Category: Biotechnology Authors: Fengying Yan Xinxun Xiao Chen Long Lin Tang Chongwei Wang Mingqing Zhang Jin Zhang Haoran Lin Hai Huang Yong Zhang Shuisheng Li Source Type: research
Toll-interacting protein negatively regulated innate immune response via NF- κB signal pathway in blunt snout bream, Megalobrama amblycephala
Dev Comp Immunol. 2022 Nov 22:104595. doi: 10.1016/j.dci.2022.104595. Online ahead of print.ABSTRACTToll-interacting protein (Tollip) is an important negative regulator of Toll-like receptor-mediated innate immunity by preventing excessive proinflammatory responses. The structure and function of Tollip have been well identified in mammals, but the piscine Tollip remains poorly understood. In the present study, a homologue of Tollip was identified and characterized from blunt snout bream (named MaTollip), which was composed of an 831 bp open reading frame encoding a protein of 276 amino acids. Phylogenetic analysis indicate...
Source: Developmental and Comparative Immunology - November 25, 2022 Category: Allergy & Immunology Authors: Wenjun Wang Yang Liu Ying Mao Yandong Xu Zuzhen Wang Ru Zhang Bing Liu Kuanyu Xia Moci Yang Jinpeng Yan Source Type: research
Functions of Membrane Progesterone Receptors (mPRs, PAQRs) in Nonreproductive Tissues
Endocrinology. 2022 Aug 30:bqac147. doi: 10.1210/endocr/bqac147. Online ahead of print.ABSTRACTGender differences in a wide variety of physiological parameters have implicated the ovarian hormones, estrogens and progesterone, in the regulation of numerous nonreproductive tissue functions. Rapid, nongenomic (nonclassical) progesterone actions mediated by membrane progesterone receptors (mPRs), which belong to the progestin and AdipoQ receptor (PAQR) family, have been extensively investigated in reproductive and nonreproductive tissues since their discovery in fish ovaries 20 years ago. The five mPR subtypes (α, β, γ, δ,...
Source: Endocrinology - August 30, 2022 Category: Endocrinology Authors: Peter Thomas Yefei Pang Maria Andrea Camilletti Luca F Castelnovo Source Type: research
GSE161899 The dependency factor RRM2 at the nexus of a copy number driven regulatory network and a target for synthetic lethal interactions with replication stress checkpoint addiction in high-risk neuroblastoma siRNA_RRM2
In this study, we identified and functionally evaluated the ‘ribonucleotide reductase regulatory subunit 2’ (RRM2) as a top-ranked 2p putative co-driver and therapeutic target in high-risk neuroblastoma enforcing replicative stress resistance. RRM2 dependency was shown in vitro through knockdown in neuroblastoma cells, with transcriptome analysis reveali ng downregulation of pediatric tumor markers. Transgenic zebrafish lines expressing RRM2 in immature sympathoblasts were crossed with a dh-MYCN transgenic zebrafish line, with RRM2 overexpression dramatically increasing tumor penetrance from 20% to nearly 100%, acce...
Source: GEO: Gene Expression Omnibus - August 3, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Identification and characterization of DEAD-box RNA helicase DDX3 in rainbow trout (Oncorhynchus mykiss) and its relationship with infectious hematopoietic necrosis virus infection
This study suggests that DDX3 has an important role in host defense against IHNV infection and these results may provide new insights into IHNV pathogenesis and antiviral drug research.PMID:35840014 | DOI:10.1016/j.dci.2022.104493
Source: Developmental and Comparative Immunology - July 15, 2022 Category: Allergy & Immunology Authors: Jing-Zhuang Zhao Li-Ming Xu Guang-Ming Ren Yi-Zhi Shao Tong-Yan Lu Source Type: research
