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Source: Molecular Medicine Reports
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Total 308 results found since Jan 2013.
Downregulation of fatty acid synthase complex suppresses cell migration by targeting phospho‑AKT in bladder cancer.
Authors: Zheng SS, Gao JG, Liu ZJ, Zhang XH, Wu S, Weng BW, Wang YL, Hou SC, Jiang B
Abstract
The aim of the present study was to investigate the effect of fatty acid synthase complex (FASN) on the migration capacity of bladder transitional cell carcinoma (BTCC) cells and the involvement of matrix metalloproteinase‑9 (MMP‑9) via targeting of phospho‑AKT (p‑AKT). FASN‑specific small‑interfering RNA (FASN‑siRNA) was used to inhibit FASN gene expression in the 5637 and 253J BTCC cell lines. The knockdown efficiency of FAM‑conjugated FASN‑siRNA was confirmed by fluorescence microscopy. The migratory a...
Source: Molecular Medicine Reports - January 16, 2016 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5Â spinal nerve ligation.
Authors: Ji Q, Di Y, He X, Liu Q, Liu J, Li W, Zhang L
Abstract
Phosphodiesterase 4 (PDE4) is an adenosine cyclic 3,5-monophosphate-specific degradative enzyme, which is closely associated with the inflammatory response. Among its four subtypes (A‑D), it remains unclear which one exerts suppressive effects on inflammation and reduces neuropathic pain. The present study aimed to examine the modulation of neuroinflammation by PDE4 subtypes in the spinal cord of a rat model of L5 spinal nerve ligation (SNL)‑induced neuropathic pain. The expression levels of PDE4A‑D were measured in the lumbar spinal cords of naÃ...
Source: Molecular Medicine Reports - December 29, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Anticancer effect of eupatilin on glioma cells through inhibition of the Notch-1 signaling pathway.
In conclusion, eupatilin had an inhibitory effect on proliferation, invasion and migration, and promoted apoptosis of glioma cells through suppression of the Notch‑1 signaling pathway. Therefore, eupatilin may have potential as an effective agent for the treatment of glioma.
PMID: 26676446 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - December 20, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
CRMP‑5 interacts with actin to regulate neurite outgrowth.
Authors: Gong X, Tan M, Gao Y, Chen K, Guo G
Abstract
CRMP family proteins (CRMPs) are abundantly expressed in the developing nervous system mediating growth cone guidance, neuronal polarity and axon elongation. CRMP‑5 has been indicated to serve a critical role in neurite outgrowth. However, the detailed mechanisms of how CRMP‑5 regulates neurite outgrowth remain unclear. In the current study, co‑immunoprecipitation was used to identify the fact that CRMP‑5 interacted with the actin and tubulin cytoskeleton networks in the growth cones of developing hippocampal neurons. CRMP‑5 exhibited increased affinit...
Source: Molecular Medicine Reports - December 20, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Downregulation of caveolin‑1 upregulates the expression of growth factors and regulators in co‑culture of fibroblasts with cancer cells.
Authors: Shi XY, Xiong LX, Xiao L, Meng C, Qi GY, Li WL
Abstract
Reduced expression levels of caveolin‑1 (Cav‑1) in tumor stromal fibroblasts influences the occurrence and progression of tumors, particularly in breast cancer, but the relevant molecular mechanism is unclear. The present study aimed to clarify the potential mechanism underlying the promotion of tumor growth by reduced Cav‑1 expression levels, by investigating Cav‑1‑targeted molecules in fibroblasts and breast cancer cells. The expression of growth factors in the ESF fibroblast cell line transfected with Cav‑1 small interfering RNA (siRNA...
Source: Molecular Medicine Reports - December 11, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Survivin is expressed in degenerated nucleus pulposus cells and is involved in proliferation and the prevention of apoptosis in vitro.
Authors: Lin Y, Yue B, Xiang H, Liu Y, Ma X, Chen B
Abstract
Survivin is a unique inhibitor of apoptosis, which is frequently present within degenerated human nucleus pulposus (NP) cells. Survivin has been extensively investigated using proliferation and apoptosis assays in tumor cells; however, studies conducted on survivin in degenerative NP cells remain limited to date. The aim of the present study was to investigate survivin expression and its effects on the proliferation and apoptosis of degenerated NP cells in vitro. The expression levels of survivin in the NP cells of patients (>45 years) with lumbar di...
Source: Molecular Medicine Reports - December 11, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Connexin43 knockdown in bone marrow‑derived dendritic cells by small interfering RNA leads to a diminished T-cell stimulation.
In conclusion, the present study demonstrated that antigens present on BM‑DCs can be suppressed by connexin43 knockdown in BM‑DCs. The present study therefore presented an effective method to modulate the immunology of BM‑DCs.
PMID: 26648560 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - December 11, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Effect of vascular endothelial growth factor siRNA and wild‑type p53 co‑expressing plasmid in MDA‑MB‑231 cells.
Authors: Guo H, Li Y, Gu J, Wang Y, Liu L, Zhang P, Liu Y
Abstract
Breast cancer urgently requires improved therapeutic strategies. In the current study, a Pvp53 plasmid that co‑expressed p53 and short‑interfering RNA against vascular endothelial growth factor (si‑VEGF) was developed to replace single plasmid transfections. Whether Pvp53 exhibited improved anti‑tumor effects in breast cancer MDA‑MB‑231 cells was investigated in the present study. Pvp53 significantly reduced the Bcl‑2/Bax ratio and increased the expression of cleaved caspase‑3 and 8. Compared with p53 and si‑VEGF single transfect...
Source: Molecular Medicine Reports - November 20, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Transient transfection of macrophage migration inhibitory factor small interfering RNA disrupts the biological behavior of oral squamous carcinoma cells.
Authors: Zeng J, Quan J, Xia X
Abstract
Macrophage migration inhibitory factor (MIF) is closely associated with tumorigenesis. The present study aimed to investigate the effects of MIF on the proliferation, migration and colony formation of oral squamous cell carcinoma (OSCC), and to quantify the protein expression levels of MIF in OSCC tissue samples. Firstly, small interfering (si)RNA was used to knock down the gene expression of MIF in Tca8113, HN5 and SCC25 OSCC cells. Secondly, proliferation, migration and colony formation of the OSCC cells were determined by MTT, transmigration and colony formation assays, re...
Source: Molecular Medicine Reports - November 10, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Inhibition of the expression of aquaporin‑1 by RNA interference in pulmonary epithelial cells and its effects on water transport.
Authors: Zhang Q, Fu J, Xue X
Abstract
In the present study, the effect of aquaporin‑1 (AQP1) on fluid transportation in pulmonary epithelial cells, and the role of AQP1 in alveolar fluid clearance were investigated to provide an experimental foundation to elucidate the pathogenesis of hyperoxic lung edema. An siRNA transfection technique was used to silence AQP1 in the A549 cell line. The transfected cells were randomized into a hyperoxia exposure and an air control group, with a negative control group set for each group. Cell volume was determined using flow cytometry, and Pf values were used to determine osmo...
Source: Molecular Medicine Reports - November 10, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Adipose differentiation-related protein is not involved in hypoxia inducible factor-1-induced lipid accumulation under hypoxia.
Authors: Shen G, Ning N, Zhao X, Liu X, Wang G, Wang T, Zhao R, Yang C, Wang D, Gong P, Shen Y, Sun Y, Zhao X, Jin Y, Yang W, He Y, Zhang L, Jin X, Li X
Abstract
Increasing evidence has showed that hypoxia inducible factor-1 (HIF1) has an important role in hypoxia-induced lipid accumulation, a common feature of solid tumors; however, its role remains to be fully elucidated. Adipose differentiation‑related protein (ADRP), a structural protein of lipid droplets, is found to be upregulated under hypoxic conditions. In the present study, an MCF7 breast cancer cell line was used to study the role of ADRP in hypoxia‑...
Source: Molecular Medicine Reports - October 28, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Novel hydroxybutyl chitosan nanoparticles for siRNA delivery targeting tissue factor inhibits proliferation and induces apoptosis in human vascular smooth muscle cells.
Authors: Wan K, Li J, Li D, Ge J, Wang Y, Li X, Guo Y, Guo J, Leng M, Wang P, An Y
Abstract
Chitosan, a polysaccharide isolated from shrimp and other crustacean shells, has been widely investigated for DNA and siRNA delivery. Despite substantial effort having been made to improve chitosan as a non‑viral gene delivery vector, the application is severely limited by its poor solubility under physiological conditions. Hydroxybutyl chitosan (HBC), a modified chitosan, is soluble under neutral conditions. Tissue factor (TF) is involved in the pathogenesis of cardiovascular diseases by promoting thrombus formation and i...
Source: Molecular Medicine Reports - October 28, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
STAT3‑regulated long non‑coding RNAs lnc‑7SK and lnc‑IGF2‑AS promote hepatitis C virus replication.
Authors: Xiong Y, Jia M, Yuan J, Zhang C, Zhu Y, Kuang X, Lan L, Wang X
Abstract
Long non‑coding RNAs (lncRNAs) are a class of RNAs that do not code protein but are important in diverse biological processes. In previous years, with the application of high‑throughput sequencing, a large number of lncRNAs associated with virus infections have been identified and intensively investigated, however, there are few studies examining the association between lncRNAs and HCV replication. Previous studies have demonstrated that signal transducer and activator of transcription 3 (STAT3) is activated by the hepatitis C vi...
Source: Molecular Medicine Reports - September 4, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Potential application of injectable chitosan hydrogel treated with siRNA in chronic rhinosinusitis therapy.
In conclusion, the results of the present study suggested that injectable chitosan based hydrogel, treated with siRNA targeting VEGF, may be used as a convenient therapeutic option for chronic rhinosinusitis.
PMID: 26299569 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - August 25, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Dickkopf‑related protein 1 induces angiogenesis by upregulating vascular endothelial growth factor in the synovial fibroblasts of patients with temporomandibular joint disorders.
Authors: Jiang SJ, Li W, Li YJ, Fang W, Long X
Abstract
Angiogenesis has an important role in the progression of temporomandibular joint disorders (TMD). The aim of the present study was to explore the association between dickkopf‑related protein 1 (DKK‑1) and angiogenesis in TMD. The expression levels of DKK‑1 and vascular endothelial growth factor (VEGF) were quantified by an ELISA assay of the synovial fluid from patients with TMD. The correlation between DKK‑1 and VEGF was analyzed by Pearson correlation test. Synovial fibroblasts were isolated from patients with TMD and were subsequently treated with r...
Source: Molecular Medicine Reports - August 6, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
