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Source: Molecular Medicine Reports

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Total 308 results found since Jan 2013.

Effects of PEG anchors in PEGylated siRNA lipoplexes on in  vitro gene‑silencing effects and siRNA biodistribution in mice.
Effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene‑silencing effects and siRNA biodistribution in mice. Mol Med Rep. 2020 Nov;22(5):4183-4196 Authors: Hattori Y, Tamaki K, Sakasai S, Ozaki KI, Onishi H Abstract Polyethylene glycol (PEG)‑modifications (PEGylations) of cationic liposome/small interfering RNA complexes (siRNA lipoplexes) can enhance their systemic stability. The present study determined the effects of PEG anchors in PEGylated siRNA lipoplexes on in vitro gene‑silencing effects and siRNA biodistribution after intravenous injection. Three types of dialkyl or trial...
Source: Molecular Medicine Reports - October 3, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation.
Authors: Ji Q, Di Y, He X, Liu Q, Liu J, Li W, Zhang L Abstract Phosphodiesterase 4 (PDE4) is an adenosine cyclic 3,5-monophosphate-specific degradative enzyme, which is closely associated with the inflammatory response. Among its four subtypes (A‑D), it remains unclear which one exerts suppressive effects on inflammation and reduces neuropathic pain. The present study aimed to examine the modulation of neuroinflammation by PDE4 subtypes in the spinal cord of a rat model of L5 spinal nerve ligation (SNL)‑induced neuropathic pain. The expression levels of PDE4A‑D were measured in the lumbar spinal cords of naÃ...
Source: Molecular Medicine Reports - December 29, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effect of pre ‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection.
Effect of pre‑freezing and saccharide types in freeze‑drying of siRNA lipoplexes on gene‑silencing effects in the cells by reverse transfection. Mol Med Rep. 2020 Aug 05;: Authors: Tang M, Hu S, Hattori Y Abstract Our previous study reported that reverse (Rev)‑transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) freeze‑dried in trehalose or sucrose solution resulted in high gene‑silencing activity in cells. The current study investigated whether pre‑freezing or saccharide types present during the freeze‑drying of siRNA lipoplexes affected geneâ€...
Source: Molecular Medicine Reports - September 20, 2020 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effect of the combination of cationic lipid and phospholipid on gene-knockdown using siRNA lipoplexes in breast tumor cells and mouse lungs
Mol Med Rep. 2023 Oct;28(4):180. doi: 10.3892/mmr.2023.13067. Epub 2023 Aug 18.ABSTRACTPreviously, using three types of cationic lipids, the effect of phospholipids in liposomal formulations on gene-knockdown efficacy was determined after in vitro and in vivo transfection with small interfering RNA (siRNA)/cationic liposome complexes (siRNA lipoplexes) containing various cationic lipids and phospholipids. In the present study, six other types of cationic lipids, namely N,N-dimethyl-N-tetradecyltetradecan-1-aminium bromide, N-hexadecyl-N,N-dimethylhexadecan-1-aminium bromide (DC-1-16), 2-[bis{2-(tetradecanoyloxy)ethyl}amino...
Source: Molecular Medicine Reports - August 18, 2023 Category: Molecular Biology Authors: Yoshiyuki Hattori Min Tang Aya Aoki Momoka Ezaki Hana Sakai Kei-Ichi Ozaki Source Type: research

Potential application of injectable chitosan hydrogel treated with siRNA in chronic rhinosinusitis therapy.
In conclusion, the results of the present study suggested that injectable chitosan based hydrogel, treated with siRNA targeting VEGF, may be used as a convenient therapeutic option for chronic rhinosinusitis. PMID: 26299569 [PubMed - as supplied by publisher]
Source: Molecular Medicine Reports - August 25, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Novel hydroxybutyl chitosan nanoparticles for siRNA delivery targeting tissue factor inhibits proliferation and induces apoptosis in human vascular smooth muscle cells.
Authors: Wan K, Li J, Li D, Ge J, Wang Y, Li X, Guo Y, Guo J, Leng M, Wang P, An Y Abstract Chitosan, a polysaccharide isolated from shrimp and other crustacean shells, has been widely investigated for DNA and siRNA delivery. Despite substantial effort having been made to improve chitosan as a non‑viral gene delivery vector, the application is severely limited by its poor solubility under physiological conditions. Hydroxybutyl chitosan (HBC), a modified chitosan, is soluble under neutral conditions. Tissue factor (TF) is involved in the pathogenesis of cardiovascular diseases by promoting thrombus formation and i...
Source: Molecular Medicine Reports - October 28, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

SiRNA directed against NF ‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro.
SiRNA directed against NF‑κB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro. Mol Med Rep. 2017 Oct 04;: Authors: Wu C, Zhao J, Zhu G, Huang Y, Jin L Abstract Acute lung injury (ALI) is a condition of acute respiratory failure, characterized by diffuse pulmonary infiltrates and severe hypoxemia. During ALI, the acute phase of inflammation induces the recruitment of activated inflammatory cells, including macrophages and lymphocytes, to the damaged lesions. Nuclear factor (NF)‑κB is a key protein in many signal transduction pathways, over‑activation of which i...
Source: Molecular Medicine Reports - October 11, 2017 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine ‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells.
Antifibrotic effects of specific siRNA targeting connective tissue growth factor delivered by polyethyleneimine‑functionalized magnetic iron oxide nanoparticles on LX‑2 cells. Mol Med Rep. 2019 Nov 20;: Authors: Yu Q, Xiong X, Zhao L, Xu T, Wang Q Abstract Connective tissue growth factor (CTGF) is a possible key determinant of progressive fibrosis. Nanotechnology has been considered as a potential tool for developing novel drug delivery systems for various diseases, including liver fibrosis. The present study aimed to investigate the potential antifibrotic activity of CTGF small interfering RNA (si...
Source: Molecular Medicine Reports - November 22, 2019 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Effects of sterol derivatives in cationic liposomes on  biodistribution and gene-knockdown in the lungs of mice systemically injected with siRNA lipoplexes
Mol Med Rep. 2021 Aug;24(2):598. doi: 10.3892/mmr.2021.12237. Epub 2021 Jun 24.ABSTRACTCationic liposomes can be intravenously injected to deliver short interfering (si)RNAs into the lungs. The present study investigated the effects of sterol derivatives in systemically injected siRNA/cationic liposome complexes (siRNA lipoplexes) on gene‑knockdown in the lungs of mice. Cationic liposomes composed of 1,2‑dioleoyl‑3‑trimethylammonium‑propane or dimethyldioctadecylammonium bromide (DDAB) were prepared as a cationic lipid, with sterol derivatives such as cholesterol (Chol), β‑sitosterol, ergosterol (Ergo) or stig...
Source: Molecular Medicine Reports - June 24, 2021 Category: Molecular Biology Authors: Yoshiyuki Hattori Hiromu Saito Teruaki Oku Kei-Ichi Ozaki Source Type: research