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Total 30 results found since Jan 2013.
L-theanine induces skeletal muscle fiber type transformation by activation of prox1/CaN signaling pathway in C2C12 myotubes
Biol Chem. 2022 Jul 18. doi: 10.1515/hsz-2022-0165. Online ahead of print.ABSTRACTThe aim of this study was to investigate the effect and mechanism of L-theanine (LT) on muscle fiber type transformation in C2C12 myotubes. Our data showed that LT exhibited significantly higher slow oxidative muscle fiber expression and lower glycolytic fibers expression. In addition, LT significantly increased the activities of malate dehydrogenase (MDH) and succinic dehydrogenase (SDH), and decreased lactate dehydrogenase (LDH) activity, the calcineurin (CaN) activity and the protein expressions of nuclear factor of activated T cell 1 (NFA...
Source: Biological Chemistry - July 19, 2022 Category: Chemistry Authors: Xiaoling Chen Man Zhang Gang Jia Hua Zhao Guangmang Liu Zhiqing Huang Source Type: research
Interaction of α-synuclein and Parkin in iron toxicity on SH-SY5Y cells: implications in the pathogenesis of Parkinson's disease.
This study has demonstrated that iron in varying concentrations (up to 400 µM) causes an increase in α-synuclein content in SH-SY5Y cells associated with mitochondrial depolarization, decreased cellular ATP content and loss of cell viability during incubation up to 96 h. Knocking-down α-synuclein expression prevents cytotoxic actions of iron, which can also be prevented by cyclosporine A (a blocker of mitochondrial permeability transition pore). These results indicate that iron cytotoxicity is mediated by α-synuclein acting on mitochondria. Likewise siRNA mediated knock-down of Parkin causes an accumulation of α-s...
Source: The Biochemical Journal - February 27, 2020 Category: Biochemistry Authors: Ganguly U, Banerjee A, Chakrabarti SS, Kaur U, Sen O, Cappai R, Chakrabarti S Tags: Biochem J Source Type: research
NF κB and Kidney Injury
Conclusion
As a critical regulator of inflammation and cell survival, the NFκB pathway is a promising target for diagnosing and treating kidney diseases. For modulation of the NFκB pathway in the clinic, a number of molecules can effectively inhibit NFκB signaling by targeting the receptors, associated adaptors, IKKs, IκBs and transcriptional regulators (144). There is further clinical evidence on small-molecule inhibitors of IKKα and NIK from recent trials on anti-cancer therapies (145). These clinical trials showed that the cancer-selective pharmacodynamic response of DTP3, the co...
Source: Frontiers in Immunology - April 15, 2019 Category: Allergy & Immunology Source Type: research
Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1
ConclusionsHydrogen peroxide upregulated the expression of FUNDC1 through the activation of ERK1/2 signal to trigger a mitophagic response, giving laryngeal cancer cells a befit for survival. These findings suggested that FUNDC1 might be a potential target for the treatment of laryngeal cancer accompanied with high lipid peroxidation status.
Source: Clinical and Translational Oncology - October 3, 2018 Category: Cancer & Oncology Source Type: research
EBV transformation induces overexpression of hMSH2/3/6 on B lymphocytes and enhances γδT cell-mediated cytotoxicity via TCR and NKG2D.
This article is protected by copyright. All rights reserved.
PMID: 29512904 [PubMed - as supplied by publisher]
Source: Immunology - March 7, 2018 Category: Allergy & Immunology Authors: Yu-Mei D, Liu HY, Liu YF, Zhang Y, He W Tags: Immunology Source Type: research
Suppression of Nrf2 confers chemosensitizing effect through enhanced oxidant-mediated mitochondrial dysfunction.
In this study, we investigated the mechanism of this chemosensitizing effect.
MAIN METHODS: KKU-100 cells were used in the study. Nrf2 expression was knocked down by siRNA and expression was validated by reverse transcription and polymerase chain reaction. Cytotoxicity was assessed by sulforhodamine B method. Intracellular reactive oxygen species (ROS) was examined by fluorescent dye, dichlorofluorescin diacetate method and mitochondrial transmembrane potential was assessed by JC1 dye assay.
KEY FINDINGS: Cytotoxicity of cisplatin (Cis) in KKU-100 cells was enhanced by knockdown of Nrf2 expression. The enhanced c...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - March 5, 2018 Category: Drugs & Pharmacology Authors: Sompakdee V, Prawan A, Senggunprai L, Kukongviriyapan U, Samathiwat P, Wandee J, Kukongviriyapan V Tags: Biomed Pharmacother Source Type: research
Klotho ameliorates cyclosporine A-induced nephropathy via PDLIM2/NF-kB p65 signaling pathway.
Abstract
Klotho, an antiaging protein, can extend the lifespan and modulate cellular responses to inflammation and oxidative stress which can ameliorate chronic kidney diseases (CKD). To investigate the molecular mechanism of Klotho on inflammation in cyclosporine A (CsA) induced nephropathy, the mice were transfected with adenovirus mediated Klotho gene and treated with cyclosporine A (CsA; 30 mg/kg/day) for 4 weeks. Also, primary human renal proximal tubule epithelial cells (RPTECs) were treated with soluble Klotho protein and LPS. The results showed that Ad-klotho significantly reduced serum creatinine (Scr) a...
Source: Biochemical and Biophysical Research communications - March 14, 2017 Category: Biochemistry Authors: Jin M, Lv P, Chen G, Wang P, Zuo Z, Ren L, Bi J, Yang CW, Mei X, Han D Tags: Biochem Biophys Res Commun Source Type: research
BK Polyomavirus Replication in Renal Tubular Epithelial Cells Is Inhibited by Sirolimus, but Activated by Tacrolimus Through a Pathway Involving FKBP‐12
BK polyomavirus (BKPyV) replication causes nephropathy and premature kidney transplant failure. Insufficient BKPyV‐specific T cell control is regarded as a key mechanism, but direct effects of immunosuppressive drugs on BKPyV replication might play an additional role. We compared the effects of mammalian target of rapamycin (mTOR)‐ and calcineurin‐inhibitors on BKPyV replication in primary human renal tubular epithelial cells. Sirolimus impaired BKPyV replication with a 90% inhibitory concentration of 4 ng/mL by interfering with mTOR–SP6‐kinase activation. Sirolimus inhibition was rapid and effective up to 24...
Source: American Journal of Transplantation - December 7, 2015 Category: Transplant Surgery Authors: H. H. Hirsch, K. Yakhontova, M. Lu, J. Manzetti Tags: Original Article Source Type: research
Inhibition of ovarian cancer cell growth by a novel TAK1 inhibitor LYTAK1
Conclusions
These data reveal the therapeutic potential of LYTAK1 as an agent targeting the pro-oncogenic TAK1 in ovarian cancer.
Source: Cancer Chemotherapy and Pharmacology - August 18, 2015 Category: Cancer & Oncology Source Type: research
P‐glycoprotein is expressed and causes resistance to chemotherapy in EBV‐positive T‐cell lymphoproliferative diseases
We examined P‐gp expression in EBV‐positive cells by immunohistochemistry staining in three patients of EBV‐T‐LPDs and the expression was detected in all patients. We also examined mdr1 mRNA expression by reverse‐transcriptase polymerase‐chain reaction (RT‐PCR) in EBV‐positive tumor cells from these patients and additional three patients. The expression was detected in all examined patients. In five EBV‐T‐LPDs patients, P‐gp function was detected by Rhodamine‐123 efflux assay in these cells. The efflux was inhibited by treatment with a P‐gp inhibitor, cyclosporine A (CsA). We also examined and det...
Source: Cancer Medicine - June 1, 2015 Category: Cancer & Oncology Authors: Mayumi Yoshimori, Honami Takada, Ken‐Ichi Imadome, Morito Kurata, Kouhei Yamamoto, Takatoshi Koyama, Norio Shimizu, Shigeyoshi Fujiwara, Osamu Miura, Ayako Arai Tags: Original Research Source Type: research
Cyclosporine A and tacrolimus inhibit bladder cancer growth through down-regulation of NFATc1.
In this study, we assessed biological significance of NFAT in human bladder cancer. Immunohistochemistry detected nuclear/cytoplasmic NFATc1 signals in 14 (21.5%)/34 (52.3%), respectively, of 65 muscle-invasive bladder carcinomas and showed that patients with nuclear NFATc1-positive tumor had a significantly higher risk of disease progression (P = 0.006). In bladder cancer cell lines, cyclosporine A (CsA) and tacrolimus (FK506), immunosuppressant drugs/non-selective NFAT inhibitors, attenuated NFATc1 expression and its nuclear translocation, NFAT transcriptional activity, and the expression of cyclooxygenase-2 and c-myc, d...
Source: Oncotarget - February 6, 2015 Category: Cancer & Oncology Tags: Oncotarget Source Type: research
The role of NFATc1 in prostate cancer progression: Cyclosporine A and tacrolimus inhibit cell proliferation, migration, and invasion
CONCLUSIONSOur results suggest that NFATc1 plays an important role in prostate cancer outgrowth. Thus, NFATc1 inactivation, especially using CsA and FK506, has the potential of being a therapeutic approach for not only hormone‐naïve but also castration‐resistant prostate cancers. Prostate © 2015 Wiley Periodicals, Inc.
Source: The Prostate - January 29, 2015 Category: Urology & Nephrology Authors: Takashi Kawahara, Eiji Kashiwagi, Hiroki Ide, Yi Li, Yichun Zheng, Hitoshi Ishiguro, Hiroshi Miyamoto Tags: Original Article Source Type: research
The roles of phosphate and the phosphate carrier in the mitochondrial permeability transition pore
Publication date: January 2012 Source:Mitochondrion, Volume 12, Issue 1 Author(s): Pinadda Varanyuwatana , Andrew P. Halestrap Phosphate activation of the mitochondrial permeability transition pore (MPTP) opening is well-documented and could involve the phosphate carrier (PiC) that we have proposed is the pore's cyclophilin-D binding component. However, others have reported that following CyP-D ablation Pi inhibits MPTP opening while cyclosporine-A (CsA) inhibits MPTP opening only when Pi is present. Here we demonstrate that Pi activates MPTP opening under all energised and de-energised conditions tested while CsA inhibi...
Source: Mitochondrion - November 6, 2014 Category: Biochemistry Source Type: research
Cyclosporine A protects podocytes via stabilization of cofilin-1 expression in the unphosphorylated state
In conclusion, the antiproteinuric effect of CsA is derived from the stabilization of the podocyte actin cytoskeleton by upregulating expression of cofilin-1, which was independent of its effect on synaptopodin.
Source: Experimental Biology and Medicine - July 28, 2014 Category: Research Authors: Li, X., Zhang, X., Li, X., Wang, X., Wang, S., Ding, J. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
