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Condition: Chronic Pain
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Total 159 results found since Jan 2013.
Duloxetine suppresses BMP-4-induced release of osteoprotegerin via inhibition of the SMAD signaling pathway in osteoblasts
Biochem Cell Biol. 2021 Mar 1. doi: 10.1139/bcb-2020-0347. Online ahead of print.ABSTRACTDuloxetine, a selective serotonin-norepinephrine reuptake inhibitor, is currently recommended to use in the treatment of chronic painful disorders such as fibromyalgia, chronic musculoskeletal pain and diabetic peripheral neuropathy. We previously demonstrated that bone morphogenetic protein-4 (BMP-4) stimulates osteoprotegerin (OPG) production in osteoblast-like MC3T3-E1 cells, and that p70 S6 kinase positively regulates the OPG synthesis. The present study is aimed to investigate the effect of duloxetine on BMP-4-stimulated OPG synth...
Source: Biochemistry and Cell Biology - March 1, 2021 Category: Biochemistry Authors: Junko Tachi Takashi Onuma Shinobu Yamaguchi Woo Kim Tomoyuki Hioki Rie Matsushima-Nishiwaki Kumiko Tanabe Haruhiko Tokuda Osamu Kozawa Hiroki Iida Source Type: research
Role of calcitonin gene-related peptide in pain regulation in the parabrachial nucleus of naive rats and rats with neuropathic pain.
This study investigated the effects and mechanisms of CGRP and CGRP receptors in pain regulation in the PBN of naive and neuropathic pain rats. Chronic sciatic nerve ligation was used to model neuropathic pain, CGRP and CGRP 8-37 were injected into the PBN of the rats, and calcitonin receptor-like receptor (CLR), a main structure of CGRP receptor, was knocked down by lentivirus-coated CLR siRNA. The hot plate test (HPT) and the Randall Selitto Test (RST) was used to determine the latency of the rat hindpaw response. The expression of CLR was detected with RT-PCR and western blotting. We found that intra-PBN injecting of CG...
Source: Toxicology and Applied Pharmacology - January 29, 2021 Category: Toxicology Authors: Wang LL, Wang HB, Fu FH, Yu LC Tags: Toxicol Appl Pharmacol Source Type: research
A thermosensitive, reactive oxygen species-responsive, MR409-encapsulated hydrogel ameliorates disc degeneration in rats by inhibiting the secretory autophagy pathway
Conclusion: Secretory autophagy and associated IL-1β secretion contribute to the pathogenesis of disc degeneration, and MR409 can effectively inhibit this pathway. The ROS-responsive thermosensitive hydrogel encapsulated with MR409 is a potentially efficacious treatment for disc degeneration.
Source: Theranostics - January 15, 2021 Category: Molecular Biology Authors: Qiangqiang Zheng, Haotian Shen, Zongrui Tong, Linxiang Cheng, Yuzi Xu, Zhiyun Feng, Shiyao Liao, Xiaojian Hu, Zongyou Pan, Zhengwei Mao, Yue Wang Tags: Research Paper Source Type: research
miR ‑142‑3p targets AC9 to regulate sciatic nerve injury‑induced neuropathic pain by regulating the cAMP/AMPK signalling pathway.
miR‑142‑3p targets AC9 to regulate sciatic nerve injury‑induced neuropathic pain by regulating the cAMP/AMPK signalling pathway.
Int J Mol Med. 2020 Dec 16;:
Authors: Li X, Wang S, Yang X, Chu H
Abstract
The aim of the present study was to investigate the effects of microRNA (miR)‑142‑3p on neuropathic pain caused by sciatic nerve injury in chronic compression injury (CCI) rats, and further investigate its mechanism. Rat experiments were divided into four parts in the study. In the first part, the rats were divided into the Sham and CCI groups. The expression of miR‑142‑3p, AC9 and cAMP...
Source: International Journal of Molecular Medicine - December 16, 2020 Category: Molecular Biology Authors: Li X, Wang S, Yang X, Chu H Tags: Int J Mol Med Source Type: research
The CUL3/neddylation inhibitor MLN4924 reduces ethanol-induced locomotor sensitization and inflammatory pain allodynia in mice.
Abstract
Heterologous sensitization of adenylyl cyclase (AC) is defined by an enhanced cAMP response following persistent activation of Gαi/o-coupled receptors. This phenomenon was first observed in cellular models, and later reported in animal models of inflammatory pain or following chronic exposure to drugs of abuse including opioids and cocaine. Recently, we used genome-wide siRNA screening to identify Cullin3 signaling as a mediator of AC sensitization in cellular models. We also showed that pharmacological inhibition of Cullin3 with the neddylation inhibitor, MLN4924, abolished heterologous sensitization of...
Source: Behavioural Brain Research - December 3, 2020 Category: Neurology Authors: Ding Z, Knipp GT, van Rijn RM, Chester JA, Watts VJ Tags: Behav Brain Res Source Type: research
Neurexin-2 is a potential regulator of inflammatory pain in the spinal dorsal horn of rats.
Abstract
Chronic pain is one of the serious conditions that affect human health and remains cure still remains a serious challenge as the molecular mechanism remains largely unclear. Here, we used label-free proteomics to identify potential target proteins that regulate peripheral inflammatory pain and reveal its mechanism of action. Inflammation in peripheral tissue was induced by injecting complete Freund's adjuvant (CFA) into rat hind paw. A proteomic method was adopted to compare the spinal dorsal horn (SDH) in peripheral inflammatory pain (PIP) model rats with controls. Differential proteins were identified i...
Source: J Cell Mol Med - November 8, 2020 Category: Molecular Biology Authors: Xu L, Feng Q, Deng H, Zhang X, Ni H, Yao M Tags: J Cell Mol Med Source Type: research
Porphyric Neuropathy: Pathophysiology, Diagnosis, and Updated Management
AbstractPurpose of ReviewTo review the peripheral neurological complications of the acute hepatic porphyrias, as well as the latest advances in their pathophysiology and management.Recent FindingsThe diagnosis of porphyric neuropathy remains challenging as varying neuropathic patterns are encountered depending on disease stage, including a non-length-dependent distribution pattern. The major pathophysiologic mechanism is δ-aminolevulinic acid (ALA)–induced neurotoxicity. The less restrictive blood-nerve barrier in the autonomic ganglia and myenteric plexus may explain the frequency of dysautonomic manifestations. Recent...
Source: Current Neurology and Neuroscience Reports - October 6, 2020 Category: Neuroscience Source Type: research
Epigenetic Restoration of Voltage ‐gated Potassium Channel Kv1.2 Alleviates Nerve Injury‐induced Neuropathic Pain
AbstractVoltage ‐gated potassium channels (Kv) are important regulators of neuronal excitability for its role of regulating resting membrane potential and repolarization. Recent studies show that Kv channels participate in neuropathic pain, but the detailed underlying mechanisms are far from being clear. In the c urrent study, we used siRNA, miR‐137 agomir and antagomir to regulate the expression of Kv1.2 in spinal cord and dorsal root ganglia (DRG) of naïve and chronic constriction injury (CCI) rats. Kv currents and neuron excitability in DRG neurons were examined by patch‐clamp whole‐cell recording to verify the...
Source: Journal of Neurochemistry - July 2, 2020 Category: Neuroscience Authors: Jingjing Zhang,
Lina Rong,
Jinping Shao,
Yidan Zhang,
Yaping Liu,
Sen Zhao,
Lei Li,
Wenli Yu,
Mengya Zhang,
Xiuhua Ren,
Qingzan Zhao,
Changlian Zhu,
Huan Luo,
Weidong Zang,
Jing Cao Tags: ORIGINAL ARTICLE Source Type: research
Evaluation of the Effect of (S)-3,4-Dicarboxyphenylglycine as a Metabotropic Glutamate Receptors Subtype 8 Agonist on Thermal Nociception Following Central Neuropathic Pain.
Conclusions: The results revealed that activation of mGluR8 in PAG is not capable of improving the thermal hyperalgesia threshold. Based on the decreased expression of mGluR8 after SCI induced by clip compression injury and its significant increase after treatment of siRNA against mGluR8, this method might still hold promise as an effective treatment of neuropathic pain. It can be concluded that increased expression of mGluR8 is due to the fact that DCPG prevents the death of neurons that express these receptors.
PMID: 32460469 [PubMed - as supplied by publisher]
Source: Asian Spine Journal - May 29, 2020 Category: Orthopaedics Tags: Asian Spine J Source Type: research
PARP-1-regulated TNF- α expression in the dorsal root ganglia and spinal dorsal horn contributes to the pathogenesis of neuropathic pain in rats.
PARP-1-regulated TNF-α expression in the dorsal root ganglia and spinal dorsal horn contributes to the pathogenesis of neuropathic pain in rats.
Brain Behav Immun. 2020 Apr 10;:
Authors: Gao Y, Bai L, Zhou W, Yang Y, Zhang J, Li L, Jiang M, Mi Y, Li TT, Zhang X, Zhang W, Xu JT
Abstract
Emerging evidence has implicated poly-(ADP-ribose) polymerase 1 (PARP-1), a transcriptional coregulator, in a variety of inflammatory diseases. In the current study, the role of PARP-1 in neuropathic pain and the underlying mechanisms were investigated. Neuropathic pain was determined by assessing the paw withdrawal th...
Source: Brain, Behavior, and Immunity - April 9, 2020 Category: Neurology Authors: Gao Y, Bai L, Zhou W, Yang Y, Zhang J, Li L, Jiang M, Mi Y, Li TT, Zhang X, Zhang W, Xu JT Tags: Brain Behav Immun Source Type: research
The inhibiting role of periaqueductal gray metabotropic glutamate receptor subtype 8 in a rat model of central neuropathic pain.
In this study, we tried to assess the modulatory effect of (S)-3,4-Dicarboxyphenylglycine (DCPG), a metabotropic glutamate receptor subtype 8 (mGluR8) agonist, in a model of chronic central neuropathic pain in male rats. We used a spinal cord contusion method (T6-T8) for the induction of chronic central neuropathic pain.Male Wistar rats were randomly assigned to 5 equal groups (n = 10 per group). Clips compression injury model was used to induce chronic central neuropathic pain. Three weeks after spinal cord injury DCPG, siRNA and normal saline were administered intra-ventrolaterally to the periaqueductal gray (PAG) regi...
Source: Neurological Research - April 6, 2020 Category: Neurology Tags: Neurol Res Source Type: research
Upregulation of lncRNA-NONRATT021203.2 in the dorsal root ganglion contributes to cancer-induced pain via CXCL9 in rats.
In this study, we found that lncRNA-NONRATT021203.2 was increased in the CIP rats and that lncRNA-NONRATT021203.2-siRNA could relieve hyperalgesia in these rats. For elucidation of the underlying mechanism, we showed that lncRNA-NONRATT021203.2 could target C-X-C motif chemokine ligand 9 (CXCL9), which was increased in the CIP rats, and that CXCL9-siRNA could relieve hyperalgesia. At the same time, silencing lncRNA-NONRATT021203.2 expression decreased the mRNA and protein levels of CXCL9. Immunofluorescence analysis showed that CXCL9 was mainly expressed in the CGRP-positive and IB4-positive DRG neurons. Further research s...
Source: Biochemical and Biophysical Research communications - February 11, 2020 Category: Biochemistry Authors: Sun RM, Wei J, Wang SS, Xu GY, Jiang GQ Tags: Biochem Biophys Res Commun Source Type: research
