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Condition: Chronic Pain

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Total 159 results found since Jan 2013.

Role of Nectin-1/c-Src Signaling in the Analgesic Effect of GDNF on a Rat Model of Chronic Constrictive Injury
In this study, we aimed to examine whether the adhesion protein nectin-1 and its downstream protein c-Src are involved in neuropathic pain. We found that nectin-1 was expressed in the superficial dorsal horn of the spinal cord and that it was increased after chronic constrictive injury (CCI). Intrathecal administration of nectin-1 siRNA attenuated neuropathic pain induced by CCI via interference of the expression of nectin-1. Furthermore, we found that GDNF can downregulate the phosphorylation level of nectin-1-associated c-Src without changing the expression level of nectin-1. In summary, these data suggest that nectin-1 ...
Source: Journal of Molecular Neuroscience - July 8, 2016 Category: Neuroscience Source Type: research

Functional inhibition of chemokine receptor CCR2 by dicer-substrate-siRNA prevents pain development
Conclusion Altogether, these results validate CCR2 as a an appropriate molecular target for pain control and demonstrate that RNAi-based gene therapy represent an highly specific alternative to classical pharmacological approaches to treat central pathologies such as chronic pain.
Source: Molecular Pain - June 14, 2016 Category: Molecular Biology Authors: Begin-Lavallee, V., Midavaine, E., Dansereau, M.-A., Tetreault, P., Longpre, J.-M., Jacobi, A. M., Rose, S. D., Behlke, M. A., Beaudet, N., Sarret, P. Tags: Research Article Source Type: research

Effect of Epac1 on pERK and VEGF Activation in Postoperative Persistent Pain in Rats
This study suggests that activation of the Epac1 signal might induce local postoperative recovery process, which could be an important mechanism by which to control postoperative chronic pain. Our data suggest that therapy targeted at decreasing Epac1 levels provides promise for the prevention and treatment of chronic postoperative pain.
Source: Journal of Molecular Neuroscience - June 9, 2016 Category: Neuroscience Source Type: research

Overexpression of chloride channel-3 is associated with the increased migration and invasion ability of ectopic endometrial cells from patients with endometriosis
STUDY QUESTION Is chloride channel-3 (ClC-3) involved in regulating the biological behavior of endometrial stromal cells (ESCs)? SUMMARY ANSWER ClC-3 promotes endometriotic cell migration and invasion. WHAT IS KNOWN ALREADY ClC-3 plays a significant role in the migration and invasion of various kinds of cells. STUDY DESIGN, SIZE, DURATION An in vitro investigation of the effect of ClC-3 on the migration and invasion of ectopic ESCs from patients with endometriosis. PARTICIPANTS/MATERIALS, SETTING, METHODS The ectopic and eutopic endometrial samples from 43 female patients with endometriosis and the endometrial samples ...
Source: Human Reproduction - April 20, 2016 Category: Reproduction Medicine Authors: Guan, Y.-t., Huang, Y.-q., Wu, J.-b., Deng, Z.-q., Wang, Y., Lai, Z.-y., Wang, H.-b., Sun, X.-x., Zhu, Y.-l., Du, M.-m., Zhu, L.-y., Chen, L.-x., Wang, L.-w. Tags: Gynaecology Source Type: research

Opioid Receptor Expression in Neuropathic Pain Neurobiology
In this study, we determined the role of G9a in diminished MOR expression and opioid analgesic effects in animal models of neuropathic pain. We found that nerve injury in rats induced a long-lasting reduction in the expression level of MORs in the DRG but not in the spinal cord. Nerve injury consistently increased the enrichment of the G9a product histone 3 at lysine 9 dimethylation in the promoter of Oprm1 in the DRG. G9a inhibition or siRNA knockdown fully reversed MOR expression in the injured DRG and potentiated the morphine effect on pain hypersensitivity induced by nerve injury. In mice lacking Ehmt2 in DRG neurons, ...
Source: Journal of Biological Chemistry - April 14, 2016 Category: Chemistry Authors: Zhang, Y., Chen, S.-R., Laumet, G., Chen, H., Pan, H.-L. Tags: Molecular Bases of Disease Source Type: research

Pain: FKBP51 inhibition reduces chronic pain
Nature Reviews Drug Discovery 15, 234 (2016). doi:10.1038/nrd.2016.55 Author: Sarah Crunkhorn Polymorphisms in the gene encoding FK506 binding protein 51 (FKBP51) — a regulator of steroid hormone signalling — influence the severity of musculoskeletal pain symptoms after trauma. Here, using Fkbp51-knockout mice and siRNA-induced silencing of Fkbp51 in the mouse spinal cord, Maiarù et
Source: Nature Reviews Drug Discovery - March 31, 2016 Category: Drugs & Pharmacology Authors: Sarah Crunkhorn Tags: Research Highlight Source Type: research

Epigenetics and Pain Research
i-Fect Used to Study ImpactsOur i-Fect siRNA, miRNA and shRNA Trasfection Kit was recently used to study the impact of G9a-specific siRNA (AGUAACGGGCAUCAAUGC) on Mu Opioid Receptors: Yuhao Zhang, Shao-Rui Chen, Geoffroy Laumet, Hong Chen and Hui-Lin Pan. Nerve Injury Diminishes Opioid Analgesia through Lysine Methyltransferase-Mediated Transcriptional Repression of µ-Opioid Receptors in Primary Sensory Neurons. First Published on February 25, 2016, doi: 10.1074/jbc.M115.711812... In some SNL rats, G9a-specific siRNA (4 µg) or the negative control siRNA was administered intrathecally. G9a-specific siRNA(AGUAACGGGCAUC...
Source: Neuromics - March 24, 2016 Category: Neuroscience Tags: DOR Dorsal Root Ganglia Ga9 MOR inhibitor i-Fect Morphine Mu Opioid Receptor Neuropathic Pain Source Type: news

siRNA-mediated downregulation of GluN2B in the rostral anterior cingulate cortex attenuates mechanical allodynia and thermal hyperalgesia in a rat model of pain associated with bone cancer.
Authors: Xu Y, Wang G, Zou X, Yang Z, Wang Q, Feng H, Zhang M Abstract It has previously been suggested that the upregulation of GluN2B-containing N-methyl D-aspartate receptors (GluN2B) within the rostral anterior cingulate cortex (rACC) may contribute to the development of chronic pain. The present study used a rat model of bone cancer pain in order to investigate whether lentiviral-mediated delivery of small interfering RNAs targeting GluN2B (LV-GluN2B) could attenuate pain associated with bone cancer, by selectively decreasing GluN2B expression within the rACC. Sprague Dawley rats were inoculated with osteosarc...
Source: Experimental and Therapeutic Medicine - February 20, 2016 Category: Journals (General) Tags: Exp Ther Med Source Type: research

Downregulation of the spinal dorsal horn clock gene Per1 expression leads to mechanical hypersensitivity via c-jun N-terminal kinase and CCL2 production in mice
Publication date: Available online 23 January 2016 Source:Molecular and Cellular Neuroscience Author(s): Norimitsu Morioka, Munenori Saeki, Tatsuhiko Sugimoto, Takuya Higuchi, Fang Fang Zhang, Yoki Nakamura, Kazue Hisaoka-Nakashima, Yoshihiro Nakata Disturbances of circadian rhythm and dysregulation of clock gene expression are involved in the induction of various neurological disorder states, including chronic pain. However, the relationship between the CNS circadian-clock gene system and nociception remains poorly defined. Significant circadian oscillations of Period (Per1, Per2), Bmal1 and Cryptochrome 1 (Cry1...
Source: Molecular and Cellular Neuroscience - January 25, 2016 Category: Neuroscience Source Type: research

Delivery of siRNA, miRNA, shRNA and Plasmids Guaranteed
Potent and Frequently Published Transfection Solutions We have proven and frequently published transfection solutions. Powerful punch and versatility are now needed more than ever with the hyper growth in gene manipulation technologies like Sleeping BeautyTM and CRISPR-Cas9.We have published examples of the use of our solutions for delivering siRNA, miRNA, shRNA and plasmids both in vitro and in vivo. Here are some of your options. i-Fect ™ -A novel cationic lipid formulation specifically designed for efficient delivery of 27mer DsiRNAs(dicer substrate small Interfering RNAs)& 21mer siRNAs (small interfering RNA...
Source: siRNA and DsiRNA Transfection Efficiency - January 20, 2016 Category: Neuroscience Tags: DNA transfection i-Fect miRNA Delivery Methods p-fect Plasmid Transfection plasmids pn-Fect shRNA siRNA Source Type: news

JAB1 is Involved in Neuropathic Pain by Regulating JNK and NF-κB Activation After Chronic Constriction Injury.
This study aimed to investigate the possible involvement of JAB1. Here, employing a neuropathic pain model induced by chronic constriction injury (CCI) on rats, we reported the role of JAB1 in the maintenance of neuropathic pain. By western blot, we found that CCI markedly up-regulated JAB1 expression in the dorsal root ganglion (DRG) and spinal cord. Immunofluorescent assay demonstrated that JAB1 was extensively localized in IB4-, CGRP- and NF200-positive neurons in the injured L5 DRG, and mainly co-localized with NeuN in spinal cord. In addition, we showed that CCI induced phosphorylation of p65 and JNK in vivo. Intrathe...
Source: Neurochemical Research - December 23, 2015 Category: Neuroscience Authors: Chen Y, Chen X, Yu J, Xu X, Wei X, Gu X, Liu C, Zhang D, Xu Z Tags: Neurochem Res Source Type: research

Kv Channels and Pain Transmissiom
i-Fect TMis a Proven Tool for Gene Manipulation in Studying All Types of PainI previously posted on use of our i-Fect Transfection Kit to silence Kv Channels Receptors. This has enabled researchers to study the role of these receptors in vitro and in vivo (see:i-Fect™ Delivers Your siRNA Payload).Sample DataFigure: Figures. siRNA-mediated knockdown of Kv1.1 expression in thoracic DRG significantly increased gastric sensitivity in naive adult rats. (A) Western blots showed a significant decrease in Kv1.1 protein in thoracic DRG (T8–T12) after intrathecal treatment with Kv1.1 siRNA but not with control siRNA. s...
Source: Neuromics - December 9, 2015 Category: Neuroscience Tags: i-Fect KV receptors. i-Fect siRNA delivery in-vivo Source Type: news

Kv Channels and Acute to Chronic Pain Transistion
i-Fect TMis a Proven Tool for Gene Manipulation in Studying All Types of PainI previously posted on use of our i-Fect Transfection Kit to silence Kv Channels Receptors. This has enabled researchers to study the role of these receptors in vitro and in vivo (see:i-Fect™ Delivers Your siRNA Payload).Sample DataFigure: Figures. siRNA-mediated knockdown of Kv1.1 expression in thoracic DRG significantly increased gastric sensitivity in naive adult rats. (A) Western blots showed a significant decrease in Kv1.1 protein in thoracic DRG (T8–T12) after intrathecal treatment with Kv1.1 siRNA but not with control siRNA. s...
Source: siRNA and DsiRNA Transfection Efficiency - November 16, 2015 Category: Neuroscience Tags: chronic pain Gene Expression Analysis Gene Silencing i-Fect KV Channels KV Receptors kv1.1 Potassium Channel Subunit pain transmission Source Type: news

Brain natriuretic peptide constitutively downregulates P2X3 receptors by controlling their phosphorylation state and membrane localization
Conclusions: We demonstrated that in mouse trigeminal neurons endogenous BNP acts on NPR-A receptors to determine constitutive depression of P2X3 receptor function. Tonic inhibition of P2X3 receptor activity by BNP/NPR-A/PKG pathways occurs via two distinct mechanisms: P2X3 serine phosphorylation and receptor redistribution to non-raft membrane compartments. This novel mechanism of receptor control might be a target for future studies aiming at decreasing dysregulated P2X3 receptor activity in chronic pain.
Source: Molecular Pain - November 14, 2015 Category: Molecular Biology Authors: Anna MarchenkovaSandra VilottiElsa FabbrettiAndrea Nistri Source Type: research