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Condition: Pheochromocytoma

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Total 31 results found since Jan 2013.

GNB2 is a mediator of lidocaine-induced apoptosis in rat pheochromocytoma PC12 cells.
Abstract Lidocaine has been recognized to induce neurotoxicity. However, the molecular mechanism underlying this effect, especially the critical molecules in cells that mediated the lidocaine-induced apoptosis were unclear. In the present study, PC12 cells were administrated with lidocaine for 48h. Using MTT assay and flow cytometry, we found lidocaine significantly decreased the cell proliferation and S phases in PC12 cells with treatment concentrations, and significantly enhanced cell apoptosis with treatment concentrations. Two-dimensional gel electrophoresis (2-DE) analysis and LC-MS/MS were used to identifica...
Source: Neurotoxicology - March 22, 2016 Category: Neurology Authors: Tan Y, Wang Q, Zhao B, She Y, Bi X Tags: Neurotoxicology Source Type: research

Ganglioside GQ1b induces dopamine release through the activation of Pyk2
In conclusion, low concentration of GQ1b is able to enhance asynchronous DA release through Pyk2/ERK/Synapsin I/actin pathway. Our findings provide new insights into the role of GQ1b in neuronal communication, and implicate the potential application of GQ1b in neurological disorders.
Source: Molecular and Cellular Neuroscience - December 18, 2015 Category: Neuroscience Source Type: research

Loss of SDHB Elevates Catecholamine Synthesis and Secretion Depending on ROS Production and HIF Stabilization.
Abstract Germline mutations in genes encoding succinate dehydrogenase subunits are associated with the development of familial pheochromocytomas and paragangliomas [hereditary paraganglioma/pheochromocytoma syndrome (HPPS)]. In particular, a mutation in succinate dehydrogenase subunit B (SDHB) is highly associated with abdominal paraganglioma and subsequent distant metastasis (malignant paraganglioma), indicating the importance of SDHB genetic testing. The discovery of HPPS suggests an association among genetic mitochondrial defects, tumor development, and catecholamine oversecretion. To investigate this associati...
Source: Neurochemical Research - November 30, 2015 Category: Neuroscience Authors: Saito Y, Ishii KA, Aita Y, Ikeda T, Kawakami Y, Shimano H, Hara H, Takekoshi K Tags: Neurochem Res Source Type: research

HIF-1α Activation by Intermittent Hypoxia Requires NADPH Oxidase Stimulation by Xanthine Oxidase
by Jayasri Nanduri, Damodara Reddy Vaddi, Shakil A. Khan, Ning Wang, Vladislav Makarenko, Gregg L. Semenza, Nanduri R. Prabhakar Hypoxia-inducible factor 1 (HIF-1) mediates many of the systemic and cellular responses to intermittent hypoxia (IH), which is an experimental model that simulates O2 saturation profiles occurring with recurrent apnea. IH-evoked HIF-1α synthesis and stability are due to increased reactive oxygen species (ROS) generated by NADPH oxidases, especially Nox2. However, the mechanisms by which IH activates Nox2 are not known. We recently reported that IH activates xanthine oxidase (XO) and the resulti...
Source: PLoS One - March 9, 2015 Category: Biomedical Science Authors: Jayasri Nanduri et al. Source Type: research

Targeting of mTORC2 may have advantages over selective targeting of mTORC1 in the treatment of malignant pheochromocytoma
Abstract Recent studies have found that mammalian target of rapamycin complex 2 (mTORC2) is emerging as a potential therapeutic target in the treatment of many human cancers. However, the effects of targeting of mTORC2 on malignant pheochromocytomas (PCC) and paragangliomas (PGL) have not been reported. The aim of the study was to investigate the effects of targeting of mTORC2 on malignant PCC/PGL by comparing the inhibitory effects of targeting of mTORC2 with mTORC1 on pheochromocytoma PC12 cell in vitro and vivo. The expressions of regulatory-associated protein of mTOR (raptor) and rapamycin-insensitive companio...
Source: Tumor Biology - February 10, 2015 Category: Cancer & Oncology Source Type: research

The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells.
In this study we demonstrated that in PC12 cells the TrkA receptor interacts with the deubiquitinating enzyme USP8/UBPy in a NGF-dependent manner, and that it is deubiquitinated in vivo and in vitro by USP8. USP8 overexpression blocked NGF-induced neurites outgrowth while the overexpression of the catalytically inactive mutant USP8/UBPy(C748A) caused a marked increase of cell differentiation. Localization and biochemical experiments have point out that USP8 and TrkA partially co-localize in endosomes after NGF stimulation. Finally we have studied the role played by USP8 on TrkA turnover; using specific siRNA for USP8 we fo...
Source: Experimental Cell Research - February 4, 2015 Category: Cytology Authors: Ceriani M, Amigoni L, D'Aloia A, Berruti G, Martegani E Tags: Exp Cell Res Source Type: research

Abstract 4059: The bone morphogenic protein 7 (Bmp7) plays a pro-tumorigenic role in pheochromocytoma
Conclusions In conclusion, we demonstrated for the first time that Bmp7 promotes the migration and invasion of PCC cells. We found that endogenous Bmp7 levels are influenced by the amount of p27. Integrin beta1 seems to mediate the ability of Bmp7 signaling to promote migration and invasion of PCC cells. Bmp7 represents a novel target for therapy of PCC since the knock-down in vitro shows promising impairment of the tumorigenic phenotype. Citation Format: Ines Leinhäuser, Ines Höfig, Natasa Anastasov, Felix Beuschlein, Massimo Mannelli, Michael J. Atkinson, Natalia S. Pellegata. The bone morphogenic protein 7 (Bmp7) play...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Leinhauser, I., Hofig, I., Anastasov, N., Beuschlein, F., Mannelli, M., Atkinson, M. J., Pellegata, N. S. Tags: Tumor Biology Source Type: research

Equol is neuroprotective during focal cerebral ischemia and reperfusion that involves p-Src and gp91phox.
Abstract Both of gp91(phox) (an isoform of nicotinamide adenine dinucleotide phosphate reduced oxidases) and Src (a non-receptor protein tyrosine kinase) are abundantly expressed in the brain and play a prominent role in mediating ischemic alteration in neurons. The inhibitory strategy of them is believed to be the promising treatment of stroke. The present study was designed to investigate the effect of equol (0.625-2.5 mg•kg(-1), i.g. for 3 days), a predominant active metabolite of daidzein, on neuroprotection against cerebral ischemia/reperfusion injury in rats and the underlying mechanisms. We found that equ...
Source: Current Neurovascular Research - September 7, 2014 Category: Neurology Authors: Wei YU, Wang Y, Zhou DX, Zhao LM, Li GR, Deng XL Tags: Curr Neurovasc Res Source Type: research

Overexpression of miR-210 is associated with SDH-related pheochromocytomas, paragangliomas, and gastrointestinal stromal tumours
miR-210 is a key regulator of response to hypoxia. Pheochromocytomas (PCs) and paragangliomas (PGLs) with germline SDHx or VHL mutations have pseudohypoxic gene expression signatures. We hypothesised that PC/PGLs containing SDHx or VHL mutations, and succinate dehydrogenase (SDH)-deficient gastrointestinal stromal tumours (GISTs), would overexpress miR-210 relative to non-SDH or -VHL-mutated counterparts. miR-210 was analysed by quantitative PCR in i) 39 PC/PGLs, according to genotype (one SDHA, five SDHB, seven VHL, three NF1, seven RET, 15 sporadic, one unknown) and pathology (18 benign, eight atypical, 11 malignant, two...
Source: Endocrine-Related Cancer - May 6, 2014 Category: Endocrinology Authors: Tsang, V. H. M., Dwight, T., Benn, D. E., Meyer-Rochow, G. Y., Gill, A. J., Sywak, M., Sidhu, S., Veivers, D., Sue, C. M., Robinson, B. G., Clifton-Bligh, R. J., Parker, N. R. Tags: Research Source Type: research

Molecular mechanism of 2-APB-induced Ca(2+) influx in external acidification in PC12.
In this study, to identify the possible target for the action of 2-APB we examined the pharmacological and molecular properties of [Ca(2+)]i and secretory responses to 2-APB under extracellular low pH conditions. 2-APB dose-dependently induced a [Ca(2+)]i increase and dopamine release, which were greatly enhanced by the external acidification (pH 6.5). [Ca(2+)]i and secretory responses to 2-APB at pH 6.5 were inhibited by the removal of extracellular Ca(2+) and SOC channel blockers such as SK&F96365, La(3+) and Gd(3+). PC12 expressed all SOC channel molecules, Orai 1, Orai 2 and Orai 3. When we used an siRNA system, do...
Source: Experimental Cell Research - March 11, 2014 Category: Cytology Authors: Takahashi K, Yokota M, Ohta T Tags: Exp Cell Res Source Type: research

Spatiotemporal expression of KHSRP modulates Schwann cells and neuronal differentiation after sciatic nerve injury.
In conclusion, we speculated that KHSRP was involved in SCs and neuronal differentiation by inducing β-catenin mRNA decay. PMID: 24368152 [PubMed - as supplied by publisher]
Source: The International Journal of Biochemistry and Cell Biology - December 21, 2013 Category: Biochemistry Authors: Zhu X, Yao L, Yang X, Sun H, Guo A, Li A, Yang H Tags: Int J Biochem Cell Biol Source Type: research

Cathepsin X promotes 6-hydroxydopamine-induced apoptosis of PC12 and SH-SY5Y cells.
Abstract The cysteine carboxypeptidase cathepsin X is an important player in degenerative processes under normal aging and pathological conditions. In the present study, we investigated the potential role of cathepsin X in 6-hydroxydopamine (6-OHDA)-induced toxicity in the pheochromocytoma cell line PC12 and neuroblastoma cell line SH-SY5Y. Cells exposed to 6-OHDA demonstrated alterations in the protein level of cathepsin X and activity of cathepsin X. Downregulation of cathepsin X expression by siRNA attenuated the neuronal death caused by 6-OHDA. Treatment with specific cathepsin X inhibitor AMS36 protected cell...
Source: Neuropharmacology - August 16, 2013 Category: Drugs & Pharmacology Authors: Pišlar AH, Zidar N, Kikelj D, Kos J Tags: Neuropharmacology Source Type: research

Ser779 Phosphorylation in FGFR Regulates Differentiation Cell Biology
The FGF receptors (FGFRs) control a multitude of cellular processes both during development and in the adult through the initiation of signaling cascades that regulate proliferation, survival, and differentiation. Although FGFR tyrosine phosphorylation and the recruitment of Src homology 2 domain proteins have been widely described, we have previously shown that FGFR is also phosphorylated on Ser779 in response to ligand and binds the 14-3-3 family of phosphoserine/threonine-binding adaptor/scaffold proteins. However, whether this receptor phosphoserine mode of signaling is able to regulate specific signaling pathways and ...
Source: Journal of Biological Chemistry - May 24, 2013 Category: Chemistry Authors: Lonic, A., Powell, J. A., Kong, Y., Thomas, D., Holien, J. K., Truong, N., Parker, M. W., Guthridge, M. A. Tags: Signal Transduction Source Type: research

Ephedrine induced Thioredoxin-1 expression through β-adrenergic receptors/Cyclic AMP/protein kinase A/dopamine- and Cyclic AMP-regulated phosphoprotein signaling pathway.
In this study, we found that Eph induced Trx-1 expression, which was inhibited by propranolol (β-adrenergic receptors inhibitor), but not by phenoxybenzamine (α-adrenergic receptors inhibitor) in rat pheochromocytoma PC12 cells. Moreover, the increase of Trx-1 expression was inhibited by SQ22536 (adenylyl cyclase inhibitor) and H-89 (protein kinase A inhibitor). Interestingly, the effect of Eph on dopamine- and Cyclic AMP-regulated phosphoprotein (DARPP-32) was similar to Trx-1. Thus, the relationship between Trx-1 and DARPP-32 was further studied. The DARPP-32 siRNA significantly reduced Trx-1 expression, but Trx-1 siRN...
Source: Cellular Signalling - February 14, 2013 Category: Cytology Authors: Jia JJ, Zeng XS, Li Y, Ma S, Bai J Tags: Cell Signal Source Type: research

Thioredoxin-1 was Required for CREB Activity by Methamphetamine in Rat Pheochromocytoma Cells.
In this study, we found that Trx-1 was induced by METH in rat pheochromocytoma PC12 cells. Furthermore, PI3K/Akt pathway was involved in METH-induced increase of Trx-1 expression. An increase in phosphorylated cAMP response element-binding protein (CREB) was also observed after exposure of PC12 cells to METH, which was inhibited by a PI3K inhibitor, LY294002. In addition, the siRNA targeted toTrx-1 reduced the level of phosphorylated CREB by METH, suggesting Trx-1 is necessary for increased activity of CREB by METH. The results obtained in this study showed that Trx-1 might play a role in the actions of METH. PMID: 23...
Source: Cellular and Molecular Neurobiology - December 13, 2012 Category: Cytology Authors: Lv T, Wang SD, Bai J Tags: Cell Mol Neurobiol Source Type: research