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Cancer: Prostate Cancer

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Total 879 results found since Jan 2013.

Silencing of hepsin and inosine 5-monophosphate dehydrogenase 2 by siRNA reduces prostate cancer cells proliferation
This study aimed to determine the transcript level of PCa-related genes, HPN and IMPDH2, in archived tissues. Their functional roles were further determined using an in vitro model of PCa. Total RNA extraction was done from formalin-fixed paraffin-embedded PCa tissues, and benign prostatic hyperplasia (BPH) tissues acted as the control. Quantitative real-time polymerase chain reaction (qPCR) was performed to measure HPN and IMPDH2 expression. The functional assay was performed in a prostate cancer cell line (DU145) on these two genes by silencing their RNA. We discovered a significantly higher expression of IMPDH2 in PCa s...
Source: Malaysian Journal of Pathology - April 29, 2022 Category: Pathology Authors: N A Wahab H D Dardar R Yunus Z M Zainudin N M Mokhtar Source Type: research

IGT mediated Nanog siRNA delivery in prostate cancer cells improves chemosensitization of Epirubicin in vitro
In this report, we have successfully delivered siRNA against Nanog with the help of pentafluorobenzyl modified Internal Oligo-guanidinium transporter (IGT) that has previously shown promising results in peptide and antisense morpholino delivery. Nanog downregulation in prostate cancer cell line DU145 in serum containing media led to suppression of associated proteins such as KLF4, FAK and cMyc and also enhanced the chemosensitivity of Epirubicin, an anthracycline based drug, in DU145 cells by associated MDR-1 downregulation in vitro. These results show that IGT is a promising candidate for siRNA delivery and its conjugatio...
Source: Bioorganic and Medicinal Chemistry Letters - October 9, 2022 Category: Chemistry Authors: Shalini Gupta Ujjal Das Surajit Sinha Source Type: research

Enhanced therapeutic effect of cisplatin on the prostate cancer in tumor-bearing mice by transfecting the attenuated Salmonella carrying a plasmid co-expressing p53 gene and mdm2 siRNA
Highlights: Abstract: Prostate cancer urgently needs an efficient therapy. Here we demonstrated that cisplatin combined with gene therapy by transfecting the attenuated Salmonella that carry a plasmid containing p53 gene and MDM2 siRNA provided a super-synergistic effect on the inhibition of prostate cancer growth in vivo. This synergistic therapy was associated with the induction of apoptotic cell death with a decreased Bcl2 to Bax expression ratio and increased expression of cleaved caspase 3 and caspase 9 in the prostate cancer xenograft. These results indicate that cisplatin-chemotherapy in combination with targeting t...
Source: Cancer Letters - June 21, 2013 Category: Cancer & Oncology Authors: Tao Jiang, Changdong Zhou, Junlian Gu, Yanan Liu, Lijing Zhao, Wei Li, Guanjun Wang, Yang Li, Lu Cai Tags: Research Articles Source Type: research

Mp46-15 lipid nanoparticle sirna potently silences clusterin and delays progression when combined with androgen receptor co-targeting in enzalutamide resistant prostate cancer
Suppression of androgen receptor (AR) signaling remains a therapeutic goal for castration resistant prostate cancer (CRPC) and despite newer potent AR pathway inhibitors, resistance frequently occurs. While co-targeting the AR with adaptive survival pathways is a rational goal, many biologically relevant genes including clusterin (CLU) are not druggable with small molecule inhibitors. While small interfering RNA (siRNA) offers the promise for very potent and specific gene silencing, in vivo delivery remains a major barrier.
Source: The Journal of Urology - April 1, 2015 Category: Urology & Nephrology Authors: Yoshiaki Yamamoto, Paulo Lin, Fan Zhang, Eliana Beraldi, Yoshihisa Kawai, Jeffrey Leong, Hideyasu Matsuyama, Pieter Cullis, Martin Gleave Tags: Prostate Cancer: Basic Research II Source Type: research

Cyclodextrin mediated delivery of NF-κB and SRF siRNA reduces the invasion potential of prostate cancer cells in vitro
Cyclodextrin mediated delivery of NF-κB and SRF siRNA reduces the invasion potential of prostate cancer cells in vitro Gene Therapy advance online publication, June 18 2015. doi:10.1038/gt.2015.50 Authors: J C Evans, J McCarthy, C Torres-Fuentes, J F Cryan, J Ogier, R Darcy, R W Watson & C M O’Driscoll
Source: Gene Therapy - June 18, 2015 Category: Genetics & Stem Cells Authors: J C EvansJ McCarthyC Torres-FuentesJ F CryanJ OgierR DarcyR W WatsonC M O’Driscoll Source Type: research

Folate-targeted Amphiphilic Cyclodextrin.siRNA nanoparticles for prostate cancer therapy exhibit PSMA mediated uptake, therapeutic gene silencing in vitro and prolonged circulation in vivo
In this study, a folate targeted cyclodextrin (CD) nanoparticle was prepared by co-formulating CD.siRNA complexes with DSPE-PEG5000-folate to target the prostate specific membrane antigen (PSMA). Targeted formulations showed increased uptake, relative to untargeted controls, in two prostate cancer cell lines expressing PSMA (VCaP and LNCaP). Competitive uptake studies, using excess folate, significantly reduced uptake of targeted nanoparticles in PSMA positive cell lines (p
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - July 3, 2016 Category: Nanotechnology Authors: James C. Evans, Meenakshi Malhotra, Jianfeng Guo, Joseph P. O'Shea, Karen Hanrahan, Amanda O'Neill, William Landry, Brendan T. Griffin, Raphael Darcy, R. William Watson, Caitriona M. O'Driscoll Source Type: research

Folate-targeted amphiphilic cyclodextrin.siRNA nanoparticles for prostate cancer therapy exhibit PSMA mediated uptake, therapeutic gene silencing in vitro and prolonged circulation in vivo
In this study, a folate targeted cyclodextrin (CD) nanoparticle was prepared by co-formulating CD.siRNA complexes with DSPE-PEG5000-folate to target the prostate specific membrane antigen (PSMA). Targeted formulations showed increased uptake, relative to untargeted controls, in two prostate cancer cell lines expressing PSMA (VCaP and LNCaP). Competitive uptake studies, using excess folate, significantly reduced uptake of targeted nanoparticles in PSMA positive cell lines (P
Source: Nanomedicine : Nanotechnology, Biology, and Medicine - July 3, 2016 Category: Nanotechnology Authors: James C. Evans, Meenakshi Malhotra, Jianfeng Guo, Joseph P. O'Shea, Karen Hanrahan, Amanda O'Neill, William Landry, Brendan T. Griffin, Raphael Darcy, R. William Watson, Caitriona M. O'Driscoll Source Type: research

SREBP1 siRNA enhance the docetaxel effect based on a bone-cancer dual-targeting biomimetic nanosystem against bone metastatic castration-resistant prostate cancer
Conclusion: This study presented a bone-cancer dual-targeting biomimetic nanodelivery system for bone metastatic CRPC.
Source: Theranostics - July 3, 2020 Category: Molecular Biology Authors: Jiyuan Chen, Zhenjie Wu, Weihong Ding, Chengwu Xiao, Yu Zhang, Shen Gao, Yuan Gao, Weimin Cai Tags: Research Paper Source Type: research

Mesoporous silica nanoparticles combined with AKR1C3 siRNA inhibited the growth of castration-resistant prostate cancer by suppressing androgen synthesis in  vitro and in vivo.
In this study, mesoporous silica nanoparticles (MSNs) were employed to deliver small interfering RNA targeting AKR1C3 (siAKR1C3) to downregulate AKR1C3 expression in CPRC cells. The optimal weight ratio of MSNs/siAKR1C3 was determined by a gel retardation assay. Prostate cancer cells such as VCaP cells, which intracrinally express AKR1C3, and LNCaP-AKR1C3 cells stably transfected with AKR1C3 were used to investigate the antitumour effect of MSNs-siAKR1C3. Fluorescence detection and Western blot analyses were applied to confirm the entrance of MSNs-siAKR1C3 into the cells. A SRB (Sulforhodamine B) assay was employed to asse...
Source: Biochemical and Biophysical Research communications - January 12, 2021 Category: Biochemistry Authors: Chen J, Yang Y, Xu D, Li J, Wu S, Jiang Y, Wang C, Yang Z, Zhao L Tags: Biochem Biophys Res Commun Source Type: research

GSE172094 siRNA-mediated E2F1 and E2F2 silencing in growth-stimulated prostate cancer cells
Contributors : Salma Ben-Salem ; Eduardo Cortes ; Qiang Hu ; Song Liu ; Hannelore HeemersSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo study the effect of siRNA-mediated E2F1 and E2F2 silencing in growth-stimulated prostate cancer cells.
Source: GEO: Gene Expression Omnibus - October 14, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Non-canonical Notch Signaling Regulates Actin Remodeling in Cell Migration by Activating PI3K/AKT/Cdc42 Pathway
In conclusion, our research results indicate that DAPT activates PI3K/AKT/Cdc42 signaling by non-canonical Notch pathway, and the activated Cdc42 promotes the filopodia formation and inhibits lamellipodia assembly, resulting in reduced migration of breast cancer cells. The results imply that non-canonical Notch signaling may play a very important role in the rapid response of cells to the extracellular signals. Author Contributions LG, JD, and LL designed the study and wrote and revised the manuscript. LL and LZ performed most of the experiments and data analysis. SZ, X-YZ, P-XM, Y-DM, Y-YW, YC, S-JT, and Y-JZ assisted i...
Source: Frontiers in Pharmacology - April 15, 2019 Category: Drugs & Pharmacology Source Type: research

EZH2 promotes cell proliferation by regulating the expression of RUNX3 in laryngeal carcinoma.
Abstract Enhancer of zeste homolog 2 (EZH2) is a highly conserved histone methyltransferase, which is overexpressed in different types of cancers such as breast and prostate cancer. It is reported that EZH2 can directly down-regulate RUNX3 by increasing histone H3 methylation. However, the role of EZH2 in the development and progression of laryngeal carcinoma has not yet been investigated, and the relationship between EZH2 and RUNX3 in laryngeal carcinoma is rarely reported. The current study aims to determine the role of EZH2 in the progression of laryngeal carcinoma, and investigate the interaction between EZH2 ...
Source: Molecular and Cellular Biochemistry - August 9, 2017 Category: Biochemistry Authors: Lian R, Ma H, Wu Z, Zhang G, Jiao L, Miao W, Jin Q, Li R, Chen P, Shi H, Yu W Tags: Mol Cell Biochem Source Type: research