Filtered By:
Infectious Disease: Adenoviruses

This page shows you your search results in order of relevance. This is page number 2.

Order by Relevance | Date

Total 599 results found since Jan 2013.

N-ethylmaleimide‑sensitive factor siRNA inhibits the release of Weibel-Palade bodies in endothelial cells.
Authors: Zhou Y, Yang SX, Yue YN, Wei XF, Liu Y Abstract The aim of the present study was to examine the effect of small interfering RNA (siRNA) methods on the expression of N‑ethylmaleimide sensitive factor (NSF) and Weibel‑Palade body (WPB) release in endothelial cells. A small hairpin RNA (shRNA), mediated with an adenovirus vector, was designed to target the N‑terminal functional area of NSF. Subsequently, viruses were transfected into human aortic endothelial cells. The mRNA and protein expression levels of NSF were detected using reverse transcription‑quantitative polymerase chain reaction and Western...
Source: Molecular Medicine Reports - June 11, 2016 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

Adenovirus-mediated delivery of siRNA targeting TM4SF4 attenuated liver cancer cell growth in vitro and in vivo.
In this study, we developed an adenoviral vector harboring an effective siRNA targeting TM4SF4 (AdSiTM4SF4) and identified its function in suppression of tumor cell growth. It was confirmed that TM4SF4 was overexpressed in HCC tissues compared with its paired non-tumor tissues by western blot analysis and immunohistochemistry. Remarkably, it was more abundant on the cell surface of HCC cells. The signals of ectopically expressed TM4SF4 in four cell lines dramatically localized in the plasma membrane, slightly in the cytoplasm, and absent in the nucleus, demonstrating that TM4SF4 is a membrane protein. Targeting TM4SF4 by A...
Source: Acta Biochimica et Biophysica Sinica - January 7, 2013 Category: Biochemistry Authors: Wang L, Feng J, Da L, Li Y, Li Z, Zhao M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

07 The approach to generate oncolytic adenovirus by sirna mediated e1b silencing
Conclusion In addition to knockout of the E1B coding cassette, siRNA mediated E1B also serves as an approach to prepare oncolytic adenovirus. Acknowledgements Supported by grants from UICC (ICRETT Fellowship), Guangdong Commission of Health and Family Plan (Grant No. 2014A276).
Source: Journal of Investigative Medicine - November 24, 2017 Category: Research Authors: Zhang, X., Matskova, L., Zheng, B., Ernberg, I., He, Z., Huang, Z. Tags: Abstracts Source Type: research

Bioinformatics analysis reveals four major hexon variants of human adenovirus type-3 (HAdV-3) as the potential strains for development of vaccine and siRNA-based therapeutics against HAdV-3 respiratory infections.
This study was designed to evaluate HAdV-3 hexon variants for the selection of potential vaccine candidates and the use of hexon gene as a target for designing siRNA that can be used as a therapy. Based on the data of worldwide distribution, duration of circulation, co-circulation and their percentage among all the variants, 3Hv-1 to 3Hv-4 were categorized as the major hexon variants. Phylogenetic analysis and the percentage of homology in the hypervariable regions followed by multi-sequence alignment, zPicture analysis and restriction enzyme analysis were carried out. In the phylogram, the variants were arranged in differ...
Source: Infection, Genetics and Evolution - June 21, 2020 Category: Genetics & Stem Cells Authors: Panda S, Banik U, Adhikary AK Tags: Infect Genet Evol Source Type: research

Adenovirus vector-mediated Gli1 siRNA induces growth inhibition and apoptosis in human pancreatic cancer with Smo-dependent or Smo-independent Hh pathway activation in vitro and in vivo
This study was to examine the effect of siRNA targeting Gli1, the downstream component of Hh pathway, on PC cells and to provide some insight into the underlying mechanisms. A Gli1siRNA-expressing adenovirus (Ad-U6-Gli1siRNA) was constructed, and its effect on PC cells was investigated in vitro and in vivo. Gli1 was expressed in 83.3% (20/24) PC tissues, whereas no expression was found in normal pancreatic ductal epithelium. Gli1 was expressed in SW1990 and CFPAC cells in which Smo was completely absent, as well as in PaTu8988, Panc-1 and BxPC-3 cells in which Smo was concomitantly present. Ad-U6-Gli1siRNA induced cell gro...
Source: Cancer Letters - July 18, 2013 Category: Cancer & Oncology Authors: Jiefang Guo, Jun Gao, Zhaoshen Li, Yanfang Gong, Xiaohua Man, Jing Jin, Hongyu Wu Tags: Research Articles Source Type: research

Adenovirus-mediated siRNA targeting TNF-α and overexpression of bone morphogenetic protein-2 promotes early osteoblast differentiation on a cell model of Ti particle-induced inflammatory response in vitro.
In this study, recombination adenovirus (Ad) vectors carrying both target genes [TNF-α small interfering RNA (TNF-α-siRNA) and bone morphogenetic protein 2 (BMP-2)] were synthesized and transfected into RAW264.7 macrophages and pro-osteoblastic MC3T3-E1 cells, respectively. The target gene BMP-2, expressed on pro-osteoblastic MC3T3-E1 cells and silenced by the TNF-α gene on cells, was treated with titanium (Ti) particles that were assessed by real-time PCR and Western blot. We showed that recombinant adenovirus (Ad-siTNFα-BMP-2) can induce osteoblast differentiation when treated with conditioned medium (CM) containing ...
Source: Braz J Med Biol Res - October 12, 2013 Category: Research Authors: Guo HH, Yu CC, Sun SX, Ma XJ, Yang XC, Sun KN, Jin QH Tags: Braz J Med Biol Res Source Type: research

Adenovirus-mediated siRNA targeting CXCR2 attenuates titanium particle-induced osteolysis by suppressing osteoclast formation.
CONCLUSIONS CXCR2 plays a critical role in particle-induced osteolysis, and siCXCR2 may be a novel treatment for aseptic loosening. PMID: 26939934 [PubMed - in process]
Source: Medical Science Monitor - March 6, 2016 Category: Research Tags: Med Sci Monit Source Type: research

Enhancement or inhibition of PLC γ2 expression in rat hepatocytes by recombinant adenoviral vectors that contain full-length gene or siRNA.
Enhancement or inhibition of PLCγ2 expression in rat hepatocytes by recombinant adenoviral vectors that contain full-length gene or siRNA. Biotech Histochem. 2017 Aug 24;:1-9 Authors: Chen XG, Liu YM, Lv QX, Ma J Abstract We investigated the effects of recombinant adenovirus vectors that overexpress or silence PLCγ2 on the expression of this gene during hepatocyte proliferation. Hepatocytes were isolated, identified by immunofluorescent cytochemical staining and infected by previously constructed Ad-PLCγ2 and Ad-PLCγ2 siRNA1, siRNA2 and siRNA3. Green fluorescent protein (GFP) expression was observ...
Source: Biotechnic and Histochemistry - August 24, 2017 Category: Research Authors: Chen XG, Liu YM, Lv QX, Ma J Tags: Biotech Histochem Source Type: research

Whole-genome siRNA screening for AAV permissivity Cell Biology
Viral vectors based on the adeno-associated virus (AAV) hold great promise for in vivo gene transfer; several unknowns, however, still limit the vectors’ broader and more efficient application. Here, we report the results of a high-throughput, whole-genome siRNA screening aimed at identifying cellular factors regulating AAV transduction. We identified 1,483...
Source: Proceedings of the National Academy of Sciences - September 8, 2015 Category: Science Authors: Mano, M., Ippodrino, R., Zentilin, L., Zacchigna, S., Giacca, M. Tags: Biological Sciences Source Type: research

Regulation on Toll-like Receptor 4 and Cell Barrier Function by Rab26 siRNA-loaded DNA Nanovector in Pulmonary Microvascular Endothelial Cells
In this study, we thoroughly investigated the role and novel mechanism of Rab26 in permeability and apoptosis of HPMVECs using a self-assembled Rab26 siRNA loaded DNA Y-motif nanoparticle (siRab26-DYM) and Rab26 adenovirus. We found that siRab26-DYM could be efficiently transfected into HPMVECs in a time- and dose-dependent manner. Importantly, the siRab26-DYM nanovector markedly aggravated the LPS-induced apoptosis and hyper-permeability of HPMVECs by promoting the nuclear translocation of Foxo1, and subsequent activation of Toll-like receptor 4 (TLR4) signal pathway. Overexpression of Rab26 by Rab26 adenoviruses partiall...
Source: Theranostics - September 12, 2017 Category: Molecular Biology Authors: Hongli Li, Binfeng He, Xueping Liu, Jingtong Li, Qian Liu, Weijie Dong, Zhi Xu, Guisheng Qian, Hua Zuo, Changhua Hu, Hang Qian, Chengde Mao, Guansong Wang Tags: Research Paper Source Type: research

TonEBP Suppresses the HO-1 Gene by Blocking Recruitment of Nrf2 to Its Promoter
Discussion Dynamic changes in the functional phenotype of macrophages are associated with pathogenesis of inflammatory diseases (5–7). TonEBP primes macrophages toward an M1 phenotype, which has pro-inflammatory properties. TonEBP does this by promoting expression of pro-inflammatory genes via interaction with NF-κB (36) and by binding directly to the promoter (37, 64). In addition, TonEBP suppresses expression of the anti-inflammatory cytokine IL-10 by limiting chromatin access to the promoter (37). The pro-inflammatory function of TonEBP suggests that inhibiting its expression or activation could suppres...
Source: Frontiers in Immunology - April 17, 2019 Category: Allergy & Immunology Source Type: research

High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection
Discussion The study of viral infections in vitro has provided innumerable advances to the field of virology. However, the lack of rapid and efficient screening tools has hindered research progress for some viruses, like JCPyV (Houff et al., 1983; Zu Rhein, 1983; Assetta and Atwood, 2017). To overcome this challenge, the development of high-throughput analyses is needed to help aid in the production of large data sets and generation of multiple lines of inquiry. Current methodologies for analyzing JCPyV infectivity predominantly rely on manual quantitation of infection by indirect immunodetection of viral proteins by epif...
Source: Frontiers in Microbiology - April 16, 2019 Category: Microbiology Source Type: research

Resveratrol Promotes Diabetic Wound Healing via SIRT1-FOXO1-c-Myc Signaling Pathway-Mediated Angiogenesis
Conclusion: Our findings indicate that the positive role of RES in diabetic wound healing via its SIRT1-dependent endothelial protection and pro-angiogenic effects involves the inhibition of FOXO1 and the de-repression of c-Myc expression. Introduction Diabetes mellitus is a metabolic disease with an increasing incidence worldwide (Zimmet et al., 2014). The disease often leads to the development of serious complications such as microangiopathy, mainly including retinopathy, nephropathy, neuropathy, and diabetic non-healing skin ulcers (Zheng et al., 2018). Diabetic non-healing skin ulcers such as foot ulcers are ca...
Source: Frontiers in Pharmacology - April 23, 2019 Category: Drugs & Pharmacology Source Type: research

Adeno-associated virus 9-mediated small RNA interference of TLR4 alleviates myocardial ischemia and reperfusion injury by inhibition of the NF- κB and MAPK signaling pathways in rats.
Conclusion AAV9-TLR4 siRNA has a positive effect on myocardial I/R injury by inhibiting the MAPK and NF-κB signaling pathways and can be used as a potential therapeutic method for myocardial I/R injury. PMID: 30854968 [PubMed - as supplied by publisher]
Source: Current Molecular Medicine - March 10, 2019 Category: Molecular Biology Authors: Xu T, Zhang K, Kan F, Li F, Yu B, Du W, Nie H Tags: Curr Mol Med Source Type: research