Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells
Biotech Histochem. 2021 Apr 29:1-7. doi: 10.1080/10520295.2021.1916837. Online ahead of print.ABSTRACTHuman leukocyte antigen (HLA-G) participates in immunosuppression and is useful for prenatal diagnostics. Isolation of fetal cells positive for HLA-G by HLA-G antibody conjugated nanoparticles from the cervix of pregnant women is the basis for non-invasive prenatal testing. Endocervical specimens are fixed in transport medium before isolation using antibody conjugated nanoparticles. Staining of HLA-G using MEM-G/9 antibody, however, is restricted to unfixed cells. We investigated the effect of several fixatives on the inte...
Source: Biotechnic and Histochemistry - April 29, 2021 Category: Research Authors: Elaheh Emadi Arezoo Khoradmehr Ali Shams Seyed Mehdi Kalantar Source Type: research

Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells
Biotech Histochem. 2021 Apr 29:1-7. doi: 10.1080/10520295.2021.1916837. Online ahead of print.ABSTRACTHuman leukocyte antigen (HLA-G) participates in immunosuppression and is useful for prenatal diagnostics. Isolation of fetal cells positive for HLA-G by HLA-G antibody conjugated nanoparticles from the cervix of pregnant women is the basis for non-invasive prenatal testing. Endocervical specimens are fixed in transport medium before isolation using antibody conjugated nanoparticles. Staining of HLA-G using MEM-G/9 antibody, however, is restricted to unfixed cells. We investigated the effect of several fixatives on the inte...
Source: Biotechnic and Histochemistry - April 29, 2021 Category: Research Authors: Elaheh Emadi Arezoo Khoradmehr Ali Shams Seyed Mehdi Kalantar Source Type: research

Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells
Biotech Histochem. 2021 Apr 29:1-7. doi: 10.1080/10520295.2021.1916837. Online ahead of print.ABSTRACTHuman leukocyte antigen (HLA-G) participates in immunosuppression and is useful for prenatal diagnostics. Isolation of fetal cells positive for HLA-G by HLA-G antibody conjugated nanoparticles from the cervix of pregnant women is the basis for non-invasive prenatal testing. Endocervical specimens are fixed in transport medium before isolation using antibody conjugated nanoparticles. Staining of HLA-G using MEM-G/9 antibody, however, is restricted to unfixed cells. We investigated the effect of several fixatives on the inte...
Source: Biotechnic and Histochemistry - April 29, 2021 Category: Research Authors: Elaheh Emadi Arezoo Khoradmehr Ali Shams Seyed Mehdi Kalantar Source Type: research

Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells
Biotech Histochem. 2021 Apr 29:1-7. doi: 10.1080/10520295.2021.1916837. Online ahead of print.ABSTRACTHuman leukocyte antigen (HLA-G) participates in immunosuppression and is useful for prenatal diagnostics. Isolation of fetal cells positive for HLA-G by HLA-G antibody conjugated nanoparticles from the cervix of pregnant women is the basis for non-invasive prenatal testing. Endocervical specimens are fixed in transport medium before isolation using antibody conjugated nanoparticles. Staining of HLA-G using MEM-G/9 antibody, however, is restricted to unfixed cells. We investigated the effect of several fixatives on the inte...
Source: Biotechnic and Histochemistry - April 29, 2021 Category: Research Authors: Elaheh Emadi Arezoo Khoradmehr Ali Shams Seyed Mehdi Kalantar Source Type: research

Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells
Biotech Histochem. 2021 Apr 29:1-7. doi: 10.1080/10520295.2021.1916837. Online ahead of print.ABSTRACTHuman leukocyte antigen (HLA-G) participates in immunosuppression and is useful for prenatal diagnostics. Isolation of fetal cells positive for HLA-G by HLA-G antibody conjugated nanoparticles from the cervix of pregnant women is the basis for non-invasive prenatal testing. Endocervical specimens are fixed in transport medium before isolation using antibody conjugated nanoparticles. Staining of HLA-G using MEM-G/9 antibody, however, is restricted to unfixed cells. We investigated the effect of several fixatives on the inte...
Source: Biotechnic and Histochemistry - April 29, 2021 Category: Research Authors: Elaheh Emadi Arezoo Khoradmehr Ali Shams Seyed Mehdi Kalantar Source Type: research

Appropriate fixative for MEM-G/9 staining of cultured human HLA-G-positive JEG-3 trophoblast tumor cells
Biotech Histochem. 2021 Apr 29:1-7. doi: 10.1080/10520295.2021.1916837. Online ahead of print.ABSTRACTHuman leukocyte antigen (HLA-G) participates in immunosuppression and is useful for prenatal diagnostics. Isolation of fetal cells positive for HLA-G by HLA-G antibody conjugated nanoparticles from the cervix of pregnant women is the basis for non-invasive prenatal testing. Endocervical specimens are fixed in transport medium before isolation using antibody conjugated nanoparticles. Staining of HLA-G using MEM-G/9 antibody, however, is restricted to unfixed cells. We investigated the effect of several fixatives on the inte...
Source: Biotechnic and Histochemistry - April 29, 2021 Category: Research Authors: Elaheh Emadi Arezoo Khoradmehr Ali Shams Seyed Mehdi Kalantar Source Type: research

Cardioprotective potential of melatonin, quercetin and resveratrol in an experimental model of diabetes
Biotech Histochem. 2021 Apr 28:1-6. doi: 10.1080/10520295.2021.1918766. Online ahead of print.ABSTRACTOxygen radicals participate in the pathogenesis of heart damage. Diabetes accelerates the formation of reactive oxygen species (ROS). We investigated the effects of the antioxidants, melatonin, quercetin and resveratrol, on cardiomyopathy and apoptosis in rats with streptozotocin (STZ) induced diabetes mellitus (DM). Rats were divided into five groups of seven: control, DM, DM + melatonin, DM + quercetin and DM + resveratrol. All treatments were begun with a single dose of STZ to induce diabetes and experimental treatments...
Source: Biotechnic and Histochemistry - April 28, 2021 Category: Research Authors: N Bostanc ıeri H Elbe M E şrefoğlu N Vard ı Source Type: research

Cytoplasmic phosphorylated ERK1/2 expression in patients with melanoma is associated with tumor stage and metastasis
We examined 34 surgically resected melanomas and investigated their clinicopathologic characteristics. We found immunostaining of phosphorylated ERK1/2 in all melanomas and faint staining in benign nevi. We found expression of cytoplasmic phosphorylated ERK1/2 in most melanomas; however, nuclear phosphorylated ERK1/2 expression was found in only five melanomas. Expression of cytoplasmic phosphorylated ERK1/2 was related to the tumor stage in melanoma. Nine of 10 cases of distant metastasis were positive for cytoplasmic phosphorylated ERK1/2. Our findings suggest that phosphorylated ERK1/2 expression is relevant to clinical...
Source: Biotechnic and Histochemistry - April 27, 2021 Category: Research Authors: Yen-Shuo Tseng Pei-Ru Wu Jeng-Wei Lu Yu-Fen Wang Kun-Tu Yeh Shu-Hui Lin Source Type: research

Cytoplasmic phosphorylated ERK1/2 expression in patients with melanoma is associated with tumor stage and metastasis
We examined 34 surgically resected melanomas and investigated their clinicopathologic characteristics. We found immunostaining of phosphorylated ERK1/2 in all melanomas and faint staining in benign nevi. We found expression of cytoplasmic phosphorylated ERK1/2 in most melanomas; however, nuclear phosphorylated ERK1/2 expression was found in only five melanomas. Expression of cytoplasmic phosphorylated ERK1/2 was related to the tumor stage in melanoma. Nine of 10 cases of distant metastasis were positive for cytoplasmic phosphorylated ERK1/2. Our findings suggest that phosphorylated ERK1/2 expression is relevant to clinical...
Source: Biotechnic and Histochemistry - April 27, 2021 Category: Research Authors: Yen-Shuo Tseng Pei-Ru Wu Jeng-Wei Lu Yu-Fen Wang Kun-Tu Yeh Shu-Hui Lin Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

The effects of hypothyroidism and hyperthyroidism on placental Hofbauer cells of pregnant rats
Biotech Histochem. 2021 Apr 13:1-10. doi: 10.1080/10520295.2021.1912828. Online ahead of print.ABSTRACTWe investigated the effects of maternal thyroid disorders on Hofbauer cells of both the placenta and the fetus in pregnant rats. We divided 21 rats into three groups: control group, induced hypothyroidism (hypo) group and induced hyperthyroidism (hyper) group. Hypothyroidism was induced using propylthiouracil and hyperthyroidism was induced using L-thyroxine. We measured maternal weight, maternal free thyroxine, fetal weight, fetal viability and placental morphology. At the end of the experiment, fetuses of the hypo and h...
Source: Biotechnic and Histochemistry - April 13, 2021 Category: Research Authors: G ökçen Bilici Cemre Ural Uygar Sa çik Zahide Çavdar G üven Erbil Source Type: research

Activation of cannabinoid 2 receptors by JWH-133 protects against ovarian ischemia-reperfusion injury in rats
Biotech Histochem. 2021 Apr 12:1-7. doi: 10.1080/10520295.2021.1905183. Online ahead of print.ABSTRACTWe investigated the efficacy of activated cannabinoid 2 receptors for alleviating ovarian ischemia-reperfusion injury in rats. Female Wistar albino rats were divided randomly into six groups: ischemia-reperfusion (IRG); ischemia-reperfusion + 0.2 mg/kg JWH-133 (JIRG1), ischemia-reperfusion + 1 mg/kg JWH-133 (JIRG2); ischemia-reperfusion + 5 mg/kg JWH-133 (JIRG3); solvent control, and sham control. Ovarian ischemia was established for 3 h followed by reperfusion for 3 h. Ovarian tissue was investigated using histology, immu...
Source: Biotechnic and Histochemistry - April 12, 2021 Category: Research Authors: Taylan Onat Melike Demir Caltekin Z üleyha Doğanyigit Can Turkler Emin Kaymak Mustafa Kara Ethem Serdar Yalvac Source Type: research

Impaired spermatogenesis caused by busulfan is partially ameliorated by treatment with conditioned medium of adipose tissue derived mesenchymal stem cells
Biotech Histochem. 2021 Apr 11:1-11. doi: 10.1080/10520295.2021.1905182. Online ahead of print.ABSTRACTBusulfan (BSU) is a chemotherapeutic drug that can cause subfertility or sterility in males. We investigated the effects of adipose tissue-derived mesenchymal stem cells (AT-MSC) conditioned medium (CM) (AT-MSC-CM) on histopathological and molecular characteristics of mouse testes exposed to BSU using stereology. We used adult male mice divided randomly into five groups: control, Dulbecco's modified Eagle's medium (DMEM), dimethyl sulfoxide (DMSO), BSU, and BSU + CM. Thirty-five days following BSU injection, sperm and tes...
Source: Biotechnic and Histochemistry - April 12, 2021 Category: Research Authors: Mohammad-Amin Abdollahifar Nahid Azad Maryam Faraji Sani Amir Raoofi Shabnam Abdi Abbas Aliaghaei Hojjat-Allah Abbaszadeh Vahid Ebrahimi Fatemeh Fadaei Fathabadi Faezeh Ghanimat Maryam Movahedi Source Type: research

Activation of cannabinoid 2 receptors by JWH-133 protects against ovarian ischemia-reperfusion injury in rats
Biotech Histochem. 2021 Apr 12:1-7. doi: 10.1080/10520295.2021.1905183. Online ahead of print.ABSTRACTWe investigated the efficacy of activated cannabinoid 2 receptors for alleviating ovarian ischemia-reperfusion injury in rats. Female Wistar albino rats were divided randomly into six groups: ischemia-reperfusion (IRG); ischemia-reperfusion + 0.2 mg/kg JWH-133 (JIRG1), ischemia-reperfusion + 1 mg/kg JWH-133 (JIRG2); ischemia-reperfusion + 5 mg/kg JWH-133 (JIRG3); solvent control, and sham control. Ovarian ischemia was established for 3 h followed by reperfusion for 3 h. Ovarian tissue was investigated using histology, immu...
Source: Biotechnic and Histochemistry - April 12, 2021 Category: Research Authors: Taylan Onat Melike Demir Caltekin Z üleyha Doğanyigit Can Turkler Emin Kaymak Mustafa Kara Ethem Serdar Yalvac Source Type: research

Tazarotene gel promotes healing of deep tissue injury in mice
Biotech Histochem. 2021 Apr 8:1-8. doi: 10.1080/10520295.2021.1905181. Online ahead of print.ABSTRACTWe investigated the efficacy and molecular mechanisms of tazarotene gel for healing deep tissue injury (DTI). We used male C57BL/6J mice to establish a DTI model. Animals were divided randomly into control, tazarotene gel and purilon gel groups. We injected 100 ul tazarotene gel, purilon gel or saline every 48 h for 20 days. Hematoxylin and eosin staining was used to observe pathological changes on days 14 and 21. The mRNA and protein expression of VEGF-α, TGF-β1 and HIF-1α were detected by qRT-PCR and west...
Source: Biotechnic and Histochemistry - April 8, 2021 Category: Research Authors: P P Liu R J Si X Yang Z R Zhang J Han A M Wang J Zhang Source Type: research

Changes in fluid composition and expression of ion channels in rat cervix during different phases of the estrus cycle
Biotech Histochem. 2021 Apr 8:1-14. doi: 10.1080/10520295.2021.1899285. Online ahead of print.ABSTRACTWe investigated changes in the composition of cervical fluid at different phases of the female rat reproductive cycle. Fluid was collected from the cervix of rats by direct cervical flushing and analyzed for changes in Na+ and Cl- content and osmolarity. Following sacrifice, the cervix was harvested and expressions of mRNA and protein for ENaCs, CFTR and AQPs were measured using qPCR and immunohistochemistry, respectively. Cervical fluid Na+ and Cl- content was high during estrus, but osmolarity was high during metestrus a...
Source: Biotechnic and Histochemistry - April 8, 2021 Category: Research Authors: Naguib Salleh Nurain Ismail Giribabu Nelli Kyaimon Myint Si Lay Khaing Source Type: research

Tazarotene gel promotes healing of deep tissue injury in mice
Biotech Histochem. 2021 Apr 8:1-8. doi: 10.1080/10520295.2021.1905181. Online ahead of print.ABSTRACTWe investigated the efficacy and molecular mechanisms of tazarotene gel for healing deep tissue injury (DTI). We used male C57BL/6J mice to establish a DTI model. Animals were divided randomly into control, tazarotene gel and purilon gel groups. We injected 100 ul tazarotene gel, purilon gel or saline every 48 h for 20 days. Hematoxylin and eosin staining was used to observe pathological changes on days 14 and 21. The mRNA and protein expression of VEGF-α, TGF-β1 and HIF-1α were detected by qRT-PCR and west...
Source: Biotechnic and Histochemistry - April 8, 2021 Category: Research Authors: P P Liu R J Si X Yang Z R Zhang J Han A M Wang J Zhang Source Type: research

Changes in fluid composition and expression of ion channels in rat cervix during different phases of the estrus cycle
Biotech Histochem. 2021 Apr 8:1-14. doi: 10.1080/10520295.2021.1899285. Online ahead of print.ABSTRACTWe investigated changes in the composition of cervical fluid at different phases of the female rat reproductive cycle. Fluid was collected from the cervix of rats by direct cervical flushing and analyzed for changes in Na+ and Cl- content and osmolarity. Following sacrifice, the cervix was harvested and expressions of mRNA and protein for ENaCs, CFTR and AQPs were measured using qPCR and immunohistochemistry, respectively. Cervical fluid Na+ and Cl- content was high during estrus, but osmolarity was high during metestrus a...
Source: Biotechnic and Histochemistry - April 8, 2021 Category: Research Authors: Naguib Salleh Nurain Ismail Giribabu Nelli Kyaimon Myint Si Lay Khaing Source Type: research

Tazarotene gel promotes healing of deep tissue injury in mice
Biotech Histochem. 2021 Apr 8:1-8. doi: 10.1080/10520295.2021.1905181. Online ahead of print.ABSTRACTWe investigated the efficacy and molecular mechanisms of tazarotene gel for healing deep tissue injury (DTI). We used male C57BL/6J mice to establish a DTI model. Animals were divided randomly into control, tazarotene gel and purilon gel groups. We injected 100 ul tazarotene gel, purilon gel or saline every 48 h for 20 days. Hematoxylin and eosin staining was used to observe pathological changes on days 14 and 21. The mRNA and protein expression of VEGF-α, TGF-β1 and HIF-1α were detected by qRT-PCR and west...
Source: Biotechnic and Histochemistry - April 8, 2021 Category: Research Authors: P P Liu R J Si X Yang Z R Zhang J Han A M Wang J Zhang Source Type: research

Changes in fluid composition and expression of ion channels in rat cervix during different phases of the estrus cycle
Biotech Histochem. 2021 Apr 8:1-14. doi: 10.1080/10520295.2021.1899285. Online ahead of print.ABSTRACTWe investigated changes in the composition of cervical fluid at different phases of the female rat reproductive cycle. Fluid was collected from the cervix of rats by direct cervical flushing and analyzed for changes in Na+ and Cl- content and osmolarity. Following sacrifice, the cervix was harvested and expressions of mRNA and protein for ENaCs, CFTR and AQPs were measured using qPCR and immunohistochemistry, respectively. Cervical fluid Na+ and Cl- content was high during estrus, but osmolarity was high during metestrus a...
Source: Biotechnic and Histochemistry - April 8, 2021 Category: Research Authors: Naguib Salleh Nurain Ismail Giribabu Nelli Kyaimon Myint Si Lay Khaing Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Abbreviations for dyes, stains and fluorescent probes used in biology and medicine in the 21st century. A bright future or the last gasp?
Biotech Histochem. 2021 Mar 22:1-7. doi: 10.1080/10520295.2021.1897677. Online ahead of print.ABSTRACTI summarize here the history of the use of abbreviations, mostly in subject areas related to dyes, stains and fluorescent probes used in biology and medicine. The dozen most popular abbreviations in these fields are identified and their salient characteristics noted. The pros and cons of each abbreviation are discussed with relevant citations. Certain abbreviations that are not in the list, e.g., AZAN and LN, are mentioned because they have an unusual origin; while others, i.e., INEPT and INADEQUATE are presented because t...
Source: Biotechnic and Histochemistry - March 22, 2021 Category: Research Authors: C J Cooksey Source Type: research

Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones
Biotech Histochem. 2021 Mar 16:1-9. doi: 10.1080/10520295.2021.1896780. Online ahead of print.ABSTRACTGastric outlet obstruction (GOO) is caused mainly by pyloric or duodenal blockage; gastric surgery and vagotomy are effective treatments. We investigated the short term effects of experimental GOO and truncal vagotomy (TV) on gut hormone levels. We used 8-week-old male Wistar rats divided randomly into four groups: control, GOO, TV, and GOO + TV. At the end of the experiment, blood and tissue samples of the pylorus and fundus were obtained for biochemical and immunohistochemical analysis. Gastric motility decreased in the ...
Source: Biotechnic and Histochemistry - March 16, 2021 Category: Research Authors: Aliye Sagkan Ozturk Mehmet Aydin Yesim Akayd ın Bozkurt Altug Ku çukgul Atakan Ozturk Source Type: research

Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones
Biotech Histochem. 2021 Mar 16:1-9. doi: 10.1080/10520295.2021.1896780. Online ahead of print.ABSTRACTGastric outlet obstruction (GOO) is caused mainly by pyloric or duodenal blockage; gastric surgery and vagotomy are effective treatments. We investigated the short term effects of experimental GOO and truncal vagotomy (TV) on gut hormone levels. We used 8-week-old male Wistar rats divided randomly into four groups: control, GOO, TV, and GOO + TV. At the end of the experiment, blood and tissue samples of the pylorus and fundus were obtained for biochemical and immunohistochemical analysis. Gastric motility decreased in the ...
Source: Biotechnic and Histochemistry - March 16, 2021 Category: Research Authors: Aliye Sagkan Ozturk Mehmet Aydin Yesim Akayd ın Bozkurt Altug Ku çukgul Atakan Ozturk Source Type: research

Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones
Biotech Histochem. 2021 Mar 16:1-9. doi: 10.1080/10520295.2021.1896780. Online ahead of print.ABSTRACTGastric outlet obstruction (GOO) is caused mainly by pyloric or duodenal blockage; gastric surgery and vagotomy are effective treatments. We investigated the short term effects of experimental GOO and truncal vagotomy (TV) on gut hormone levels. We used 8-week-old male Wistar rats divided randomly into four groups: control, GOO, TV, and GOO + TV. At the end of the experiment, blood and tissue samples of the pylorus and fundus were obtained for biochemical and immunohistochemical analysis. Gastric motility decreased in the ...
Source: Biotechnic and Histochemistry - March 16, 2021 Category: Research Authors: Aliye Sagkan Ozturk Mehmet Aydin Yesim Akayd ın Bozkurt Altug Ku çukgul Atakan Ozturk Source Type: research

Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones
Biotech Histochem. 2021 Mar 16:1-9. doi: 10.1080/10520295.2021.1896780. Online ahead of print.ABSTRACTGastric outlet obstruction (GOO) is caused mainly by pyloric or duodenal blockage; gastric surgery and vagotomy are effective treatments. We investigated the short term effects of experimental GOO and truncal vagotomy (TV) on gut hormone levels. We used 8-week-old male Wistar rats divided randomly into four groups: control, GOO, TV, and GOO + TV. At the end of the experiment, blood and tissue samples of the pylorus and fundus were obtained for biochemical and immunohistochemical analysis. Gastric motility decreased in the ...
Source: Biotechnic and Histochemistry - March 16, 2021 Category: Research Authors: Aliye Sagkan Ozturk Mehmet Aydin Yesim Akayd ın Bozkurt Altug Ku çukgul Atakan Ozturk Source Type: research

Short term effects of experimental gastric outlet obstruction and truncal vagotomy on gut hormones
Biotech Histochem. 2021 Mar 16:1-9. doi: 10.1080/10520295.2021.1896780. Online ahead of print.ABSTRACTGastric outlet obstruction (GOO) is caused mainly by pyloric or duodenal blockage; gastric surgery and vagotomy are effective treatments. We investigated the short term effects of experimental GOO and truncal vagotomy (TV) on gut hormone levels. We used 8-week-old male Wistar rats divided randomly into four groups: control, GOO, TV, and GOO + TV. At the end of the experiment, blood and tissue samples of the pylorus and fundus were obtained for biochemical and immunohistochemical analysis. Gastric motility decreased in the ...
Source: Biotechnic and Histochemistry - March 16, 2021 Category: Research Authors: Aliye Sagkan Ozturk Mehmet Aydin Yesim Akayd ın Bozkurt Altug Ku çukgul Atakan Ozturk Source Type: research

Effects of metformin on lipopolysaccharide induced inflammation by activating fibroblast growth factor 21
Biotech Histochem. 2021 Mar 5:1-9. doi: 10.1080/10520295.2021.1894353. Online ahead of print.ABSTRACTLipopolysaccharide (LPS) is a component of the cell wall of Gram-negative bacteria that produces endotoxemia, which may cause septic shock. Metformin (MET) is a widely used hypoglycemic drug that exhibits anti-inflammatory properties. Fibroblast growth factor 21 (FGF21) is an endocrine polypeptide that affects glucose and lipid metabolism, and also possesses anti-inflammatory properties. We investigated the effects of MET and FGF21 on inflammation due to LPS induced endotoxemia in male rats. Animals were divided into five g...
Source: Biotechnic and Histochemistry - March 5, 2021 Category: Research Authors: Ezgi Kar Özkan Alataş Varol Şahıntürk Semih Öz Source Type: research

Effects of metformin on lipopolysaccharide induced inflammation by activating fibroblast growth factor 21
Biotech Histochem. 2021 Mar 5:1-9. doi: 10.1080/10520295.2021.1894353. Online ahead of print.ABSTRACTLipopolysaccharide (LPS) is a component of the cell wall of Gram-negative bacteria that produces endotoxemia, which may cause septic shock. Metformin (MET) is a widely used hypoglycemic drug that exhibits anti-inflammatory properties. Fibroblast growth factor 21 (FGF21) is an endocrine polypeptide that affects glucose and lipid metabolism, and also possesses anti-inflammatory properties. We investigated the effects of MET and FGF21 on inflammation due to LPS induced endotoxemia in male rats. Animals were divided into five g...
Source: Biotechnic and Histochemistry - March 5, 2021 Category: Research Authors: Ezgi Kar Özkan Alataş Varol Şahıntürk Semih Öz Source Type: research

Effects of metformin on lipopolysaccharide induced inflammation by activating fibroblast growth factor 21
Biotech Histochem. 2021 Mar 5:1-9. doi: 10.1080/10520295.2021.1894353. Online ahead of print.ABSTRACTLipopolysaccharide (LPS) is a component of the cell wall of Gram-negative bacteria that produces endotoxemia, which may cause septic shock. Metformin (MET) is a widely used hypoglycemic drug that exhibits anti-inflammatory properties. Fibroblast growth factor 21 (FGF21) is an endocrine polypeptide that affects glucose and lipid metabolism, and also possesses anti-inflammatory properties. We investigated the effects of MET and FGF21 on inflammation due to LPS induced endotoxemia in male rats. Animals were divided into five g...
Source: Biotechnic and Histochemistry - March 5, 2021 Category: Research Authors: Ezgi Kar Özkan Alataş Varol Şahıntürk Semih Öz Source Type: research