Topical administration of hydroethanolic extract of Trifolium pratense (red clover) accelerates wound healing by apoptosis and re-epithelialization.
Abstract We investigated the wound healing effects of an ointment prepared from a hydroethanolic extract of Trifolium pratense (red clover) in Wistar rats. An animal model was prepared by making 314 mm2 full thickness skin excisions in all animals. Rats were divided into four experimental groups. Group 1 was a non-intervention control. Groups 2, 3 and 4 were treated topically with ointments containing 1.5, 3.0 and 6% (w/v) T. pretense extract, respectively. We evaluated the effects of these ointments by histological analysis of collagen production, epidermis thickness, fibroblast distribution and wound contraction...
Source: Biotechnic and Histochemistry - July 27, 2020 Category: Research Authors: Manzoureh R, Farahpour MR Tags: Biotech Histochem Source Type: research

Distribution of spleen connective tissue fibers in diabetic and vitamin C treated diabetic rats.
Ouml;zer Ç Abstract We investigated the distribution of connective tissue fibers in diabetic and vitamin C treated diabetic rat spleen. Rats were divided into three groups: group A, control; group B, diabetic; group C, vitamin C treated diabetic. Diabetes was induced by streptozotocin. Vitamin C was administered intragastrically for 21 days. Spleen tissues were examined by light microscopy after staining with Masson's trichrome, Gomori silver impregnation and van Gieson. In group B, we found accumulation of collagen fibers in the trabeculae, in the capsule and around the central artery and splenic sinu...
Source: Biotechnic and Histochemistry - July 22, 2020 Category: Research Authors: Özerkan D, Özsoy N, Cebesoy S, Özer Ç Tags: Biotech Histochem Source Type: research

Correction.
Authors: PMID: 32689811 [PubMed - as supplied by publisher] (Source: Biotechnic and Histochemistry)
Source: Biotechnic and Histochemistry - July 21, 2020 Category: Research Tags: Biotech Histochem Source Type: research

The hydroalcoholic extract of Zingiber officinale diminishes diazinon-induced hepatotoxicity by suppressing oxidative stress and apoptosis in rats.
Abstract Diazinon (DZN) is an organophosphate insecticide that affects the liver adversely. Ginger exhibits antioxidant properties. We investigated the hepatoprotective effects of an ethanolic extract of ginger root on DZN induced hepatotoxicity. We measured total phenolics and flavonoids in the hydroalcoholic extract. We used Wistar rats divided into four groups: control, 100 mg/kg/day ginger by gavage, 10 mg/kg/day DZN intraperitoneally, and ginger + DZN group treated with ginger 1 h before DZN. All treatments were for 30 consecutive days. One day after the last treatment, we evaluated oxidative stress para...
Source: Biotechnic and Histochemistry - July 16, 2020 Category: Research Authors: Yaghubi Beklar S, Hamzeh M, Karimpour Malekshah A, Talebpour Amiri F Tags: Biotech Histochem Source Type: research

Dynamics of breast tumor incidence, tumor volume and serum metabolic hormones in calorie restricted rats.
Abstract Reports of the effects of calorie restriction on tumors after the tumor has developed are uncommon and limited to a few tumor types. We investigated the effects of calorie restriction on tumor growth in breast cancer after tumor growth had progressed. We used the N-nitroso-N-methylurea (NMU) induced breast cancer model in rats. In addition to a healthy control group (C), rats with 10 - 12 mm tumors were divided into three groups: cancer control group (CC), alternate day feeding group (ADF) and calorie restriction group (CR-50%). At the end of the experimental period the volume of tumors was decr...
Source: Biotechnic and Histochemistry - July 16, 2020 Category: Research Authors: Koc Yildirim E, Balkaya M Tags: Biotech Histochem Source Type: research

Bavachinin mitigates DMH induced colon cancer in rats by altering p53/Bcl2/BAX signaling associated with apoptosis.
Abstract Bavachinin is a flavanone obtained from the Chinese herb, Fructus Psoraleae. Flavonoids and flavanones are recognized as cancer preventive agents. We investigated the anticancer properties of bavachinin using a model of dimethylhydrazine (DMH and dextran sodium sulfate (DSS) induced rat colon cancer. We investigated aberrant crypt foci (ACF), hyperplastic lesions, catalase (CAT), superoxide dismutase (SOD) and glutathione (GST) levels in Wistar rats. Expression of cancer biomarkers including IL-6, p53, Bcl2 and BAX was investigated. We found that bavachinin administered to rats re-established the colonic ...
Source: Biotechnic and Histochemistry - July 15, 2020 Category: Research Authors: Zhao C, Ghosh B, Chakraborty T, Roy S Tags: Biotech Histochem Source Type: research

Effects of diabetes on nitric oxide synthase in rat uterus.
Abstract Diabetes mellitus (DM) is a common metabolic disorder with complications including nephropathy, cardiomyopathy, neuropathy and infertility. We investigated nitric oxide synthase (NOS) expression in uterine tissue of diabetic rats. The rats were assigned randomly to four groups of ten: group C, control; group 1, diabetic for 1 month; group 2, diabetic for 2 months; group 3, diabetic for 3 months. DM was induced by streptozotocin injection. nNOS, iNOS, eNOS expressions and TUNEL staining were assessed in uterine tissue and luteinizing hormone (LH), follicle stimulating hormone (FSH) and estro...
Source: Biotechnic and Histochemistry - July 13, 2020 Category: Research Authors: Karabulut D, Sonmez MF Tags: Biotech Histochem Source Type: research

Metformin alleviates experimental colitis in mice by up-regulating TGF- β signaling.
Metformin alleviates experimental colitis in mice by up-regulating TGF-β signaling. Biotech Histochem. 2020 Jul 13;:1-7 Authors: Liu X, Sun Z, Wang H Abstract Ulcerative colitis is an inflammatory condition of the colon. Metformin, an antidiabetic drug, can suppress the inflammatory cascade. We investigated the effects of metformin on experimental colitis. Experimental colitis was induced in mice using dextran sulfate sodium. We used three groups of mice: untreated control, saline treated and metformin treated. We measured weight, disease activity index, colon length, levels of inflammatory cytok...
Source: Biotechnic and Histochemistry - July 13, 2020 Category: Research Authors: Liu X, Sun Z, Wang H Tags: Biotech Histochem Source Type: research

Hematoxylin in the 21st century.
Abstract Hematoxylin continues to be a popular substance in the 21st century. I present here some recent developments including the use and misuse of the terms, hematoxylin and hemalum, in biological staining. The medical use of hematoxylin and its application as a dye for hair or textiles continues to be of interest. Unusual applications including its use as a biosensor and for study of vibrational properties of wood are included. PMID: 32648784 [PubMed - as supplied by publisher] (Source: Biotechnic and Histochemistry)
Source: Biotechnic and Histochemistry - July 10, 2020 Category: Research Authors: Cooksey CJ Tags: Biotech Histochem Source Type: research

Effects of carbon dioxide pneumoperitoneum on exocrine and endocrine functions, and oxidative state of rat pancreas.
Abstract We investigated the effects of increased intra-abdominal pressure during laparoscopy on the endocrine and exocrine functions, oxidative stress and histopathology of the pancreas in rats. We established three experimental groups of eight animals. Group 1 was the untreated control. Forth other two groups, pneumoperitoneum with CO2 was established for 60 min at 6 mm Hg for group 2 and 12 mm Hg for group 3; groups 2 and 3 animals were allowed to re-perfuse for 30 min. Amylase, glucagon and insulin levels were analyzed in blood samples and insulin:glucagon ratio was calculated. Histopathology and mal...
Source: Biotechnic and Histochemistry - July 9, 2020 Category: Research Authors: Sag S, Imamoglu M, Sarihan H, Yulug E, Alver A, Geze Saatci S, Cay A Tags: Biotech Histochem Source Type: research

A histopathological and stereological study of the effects of acetylsalicylic acid on doxorubicin-induced hepatotoxicity in mice.
Yılmaz B Abstract Doxorubicin (Dox) is an anthracycline antibiotic with antineoplastic activity. Acetylsalicylic acid (Asa) is recommended for use as a prophylactic for thromboembolism during treatment of cancers. We investigated liver toxicity due to combined use of Dox and Asa in chemotherapy regimens. We used 140 Swiss albino mice divided into four main groups: control, Dox, Asa, and Dox + Asa. Each group was subdivided into seven subgroups based on time of sacrifice, i.e., 6, 12, 24, 48 h and 7, 14, 21 days. Quantitative and histopathological changes in liver were assessed by light microscopy and st...
Source: Biotechnic and Histochemistry - July 9, 2020 Category: Research Authors: Gökçe AB, Eren B, Sağır D, Demirel Yılmaz B Tags: Biotech Histochem Source Type: research

Immunohistochemical profile of the anti-apoptosis, apoptosis and proliferation markers Bcl-2, caspase-3, p53, and Ki-67 in botryoid odontogenic cysts compared to lateral periodontal cysts and gingival cysts of the adult.
Abstract We investigated the differences in growth and rates of recurrence of the botryoid odontogenic cyst (BOC) and the less aggressive lateral periodontal cyst (LPC) and gingival cyst of the adult (GCA). We compared the immunohistochemical expression of selected biomarkers of apoptosis and proliferation and of regulators of their activity. Sections from archival paraffin blocks of 15 BOCs, six GCAs, six LPCs, and three odontogenic keratocysts (OKCs) were processed for immunohistochemical localization of Bcl-2, caspase-3, p53 and Ki-67. Labeled and unlabeled epithelial cells were counted and differences in the m...
Source: Biotechnic and Histochemistry - July 9, 2020 Category: Research Authors: Basile JR, Castle JT, Redman RS Tags: Biotech Histochem Source Type: research

Silencing Stat3 inhibits viability and induces apoptosis in BGC-823 human gastric cancer cell line.
Abstract Gastric cancer (GC) is characterized by infrequent early diagnosis, poor prognosis and high mortality. Signal transducer and activator of transcription 3 (Stat3) and signal transducer and activator of transcription 5b (Stat5b) play multiple roles in the development and progression of many human cancers. We investigated the effects of silencing Stat3 and Stat5b on the viability and apoptosis of the human gastric cancer cell line, BGC-823. We found that Stat3 and Stat5b were expressed in both the nucleus and cytoplasm of BGC-823 cells. Silencing of Stat3 caused significantly decreased viability and increase...
Source: Biotechnic and Histochemistry - July 3, 2020 Category: Research Authors: Xu Y, Feng S, Niu B Tags: Biotech Histochem Source Type: research

Physiochemical properties of copper doped calcium sulfate in vitro and angiogenesis in vivo.
We examined physical and physicochemical properties of the copper doped calcium sulfates and the effects of the cements on angiogenesis in vivo. Rod shaped calcium sulfate crystals were visible by scanning electron microscopy in the cement that contained no copper sulfate, whereas plate-like crystals covered the surface of the cement with high copper content. After immersion of the cements in simulated body fluid (SBF) for 1 day, X-ray diffractometric analysis showed that gypsum precipitates had formed in the copper doped calcium sulfate. The compressive strength of the cements increased from 3.3 MPa for pure calcium sulfa...
Source: Biotechnic and Histochemistry - July 2, 2020 Category: Research Authors: Huang L, Xie YH, Xiang HB, Hou YL, Yu B Tags: Biotech Histochem Source Type: research

Micro- and macroanatomical features of the uropygial gland of duck (Anas platyrhynchos) and pigeon (Columba livia).
We describe the morphological, histological and histochemical characteristics of the uropygial gland of the duck (Anas platyrhynchos) and pigeon (Columba livia). The weight and dimensions of the glands were recorded. The gland openings onto the papilla in ducks were surrounded by double tufts of downy feathers; the tufts were held together by an oily secretion. In the pigeon, the gland opening was onto naked skin. Samples for light and transmission electron microscopy were obtained from adult birds. Gland morphometry of the duck showed greater dimensions and relative gland weight than the pigeon. In both species the gland ...
Source: Biotechnic and Histochemistry - June 30, 2020 Category: Research Authors: Hassanin A, Shoeib M, Massoud D Tags: Biotech Histochem Source Type: research

Early morphological and apoptotic responses of bird erythrocytes to thermal stress.
Abstract Little is known about early indicators of heat stress in bird erythrocytes. We investigated the effects of elevated temperatures on the morphology and cellular responses of avian erythrocytes. Hen red blood cells were subjected to 22-45 °C temperatures for 1 h and 4 h, then stained and examined by light microscopy to assess morphological alterations. Cell viability, cytotoxicity and caspases 3 and 7 activity also were investigated. We found that short-term exposure of hen blood to 43-45 °C caused morphological alterations and increased the activity of pro-apoptotic caspases 3 a...
Source: Biotechnic and Histochemistry - June 29, 2020 Category: Research Authors: Szabelak A, Bownik A, Knaga S, Kasperek K Tags: Biotech Histochem Source Type: research

Morin treatment for acute ethanol exposure in rats.
Abstract Ethanol intoxication increases oxidative stress and pro-inflammatory proteins, which cause neurodegeneration. Morin is a natural flavonoid obtained from plants of the Moraceae family that exhibits antioxidant, anti-inflammatory, hepatoprotective, anticancer and cardioprotective properties. We investigated the neuroprotective effect of morin on ethanol intoxicated rats. Rats exposed to ethanol exhibit increased cholesterol, triglycerides, phospholipids, free fatty acids, and lipid oxidative byproducts, and decreased the activity of antioxidant enzymes and membrane ATPase. We found that ethanol increased ac...
Source: Biotechnic and Histochemistry - June 29, 2020 Category: Research Authors: Singaravelu A, Venkatachalam K, Jayaraj RL, Jayabalan P, Nadanam S Tags: Biotech Histochem Source Type: research

Investigation of EGFR/pi3k/Akt signaling pathway in seminomas.
ro LA Abstract Activation of the receptor for epidermal growth factor (EGFR) in some testicular tumors activates several signaling pathways. Some components of these pathways are phosphorylated or mutated in testicular germ tumors (TCGT), including EGFR, Kirstein ras oncogen (KRAS) and cell surface protein of the germ cell (KIT). The latter two activate RAF ⁄MEK⁄ERK and PI3 K⁄AKT, and interconnect with the EGFR/pI3 k/Akt pathway. We investigated the expression of EGFR/pI3 k/Akt pathway proteins in seminomas and in their precursor lesion, germinal cell neoplasia in situ (GCNIS) and ...
Source: Biotechnic and Histochemistry - June 29, 2020 Category: Research Authors: Guerra F, Quintana S, Giustina S, Mendeluk G, Jufe L, Avagnina MA, Díaz LB, Palaoro LA Tags: Biotech Histochem Source Type: research

Effects of karanjin on dimethylhydrazine induced colon carcinoma and aberrant crypt foci are facilitated by alteration of the p53/Bcl2/BAX pathway for apoptosis.
Abstract We investigated the effects of karanjin on dimethylhydrazine (DMH) induced colon cancer in rats. Male Wistar rats were injected with DMH followed by dextran sodium sulfate in drinking water for 7 days. Karanjin at doses of 50,100 and 200 mg/kg was administered orally for 18 weeks. Colon tissues were investigated using TUNEL analysis of apoptosis; histopathological assessment including number of aberrant crypt foci (ACF); immunohistochemical staining for Bcl-2-associated X protein (BAX), B-cell lymphoma 2 (Bcl2), p53 and proliferating cell nuclear antigen (PCNA); and antioxidant assay in vivo. We...
Source: Biotechnic and Histochemistry - June 25, 2020 Category: Research Authors: Zhang J, Xie Y, Fan Q, Wang C Tags: Biotech Histochem Source Type: research

Effects of carnosine on apoptosis, transient receptor potential melastatin 2, and betatrophin in rats exposed to formaldehyde.
Abstract We investigated the effects of exposure to formaldehyde on transient receptor potential melastatin 2, betatrophin, total oxidant status and total antioxidant status in rat liver and kidney tissues. We also investigated the effects of carnosine on formaldehyde treated animals. We used 28 male rats divided ramdomly into four groups of seven: untreated control group, carnosine treated group, formaldehyde treated group and formaldehyde + carnosine group. The experiment lasted for four weeks. Betatrophin levels in samples were measured uing the enzyme-linked immunosorbent assay, and total oxidant status and to...
Source: Biotechnic and Histochemistry - June 25, 2020 Category: Research Authors: Akkoc RF, Ogeturk M, Aydin S, Kuloglu T, Aydin S Tags: Biotech Histochem Source Type: research

An immunohistochemical study of the effects of orexin receptor blockade on phospholipase C- β3 level in rat hippocampal dentate gyrus neurons.
An immunohistochemical study of the effects of orexin receptor blockade on phospholipase C-β3 level in rat hippocampal dentate gyrus neurons. Biotech Histochem. 2020 Jun 25;:1-6 Authors: Mousavi Z, Kourosh-Arami M, Mohsenzadegan M, Komaki A Abstract Orexin-A (hypocretin-1) is a neuropeptide that is produced in the lateral hypothalamic area (LHA) and promotes widespread cortical activation. We investigated the effect of SB-334867, a selective orexin receptor 1 (OXR1) antagonist, on phospholipase C-β3 (PLCβ3) level using slices of rat hippocampus preparations and immunohistochemistry. We ...
Source: Biotechnic and Histochemistry - June 25, 2020 Category: Research Authors: Mousavi Z, Kourosh-Arami M, Mohsenzadegan M, Komaki A Tags: Biotech Histochem Source Type: research

Effect of geldanamycin on the expression of the matrix molecules and angiogenetic factors in a gastric cancer cell line.
We examined cancer cells on laminin and collagen I coated surfaces to determine their response to the angiogenic effect of these matrix molecules. We also evaluated the expression levels of VEGF, MMP-9, ES and TSP-1 using ELISA. We found that application of 17-AAG to the gastric cancer cell line on culture dish plastic decreased VEGF, TSP-1, ES and MMP-9 expression, whereas of all of these proteins were increased by laminin and collagen coating. 17-AAG currently is in clinical trial phase 2 and may be a promising drug for treatment of gastric cancer. PMID: 32573272 [PubMed - as supplied by publisher] (Source: Biotechn...
Source: Biotechnic and Histochemistry - June 23, 2020 Category: Research Authors: Gürpınar T, Kosova F, Kurt FO, Cambaz SU, Yücel AT, Umur N, Tuğlu MI Tags: Biotech Histochem Source Type: research

Rapid, practical and safe isolation of adipose derived stem cells.
E, Erdost H Abstract We compared migration, proliferation, growth curve, confluency and differentiation into adipogenic, osteogenic and chondrogenic cell lineages of mesenchymal stem cells derived from adipose tissue cultured in scratched and nonscratched cell culture flasks. Mesenchymal stem cells were isolated from rat adipose tissue using a nonenzymatic method. We investigated two groups. For the control group, minced adipose tissue was implanted conventionally onto the surface of standard plastic cell culture flasks. For the experimental group, the tissues were cultured in flasks with a scratched surface. We f...
Source: Biotechnic and Histochemistry - June 23, 2020 Category: Research Authors: Çerçi E, Erdost H Tags: Biotech Histochem Source Type: research

Copy number variation, gene expression and histological localization of human beta-defensin 2 in patients with adeno-tonsillar hypertrophy.
Abstract Both bacterial infections and innate oral immunity response participate in development of adeno-tonsillar hypertrophy (ATH). ATH can lead to obstructive sleep apnea. We investigated the beta-defensin 2 (hBD-2) encoding gene, DEFB4, by analyzing the copy number variations (CNVs) of the defensin gene cluster in patients with ATH and by correlating CNV with DEFB4 gene expression. We enrolled 79 patients with ATH, 21 of whom presented with only adenoid hypertrophy, while 58 exhibited hypertrophy of both adenoid and tonsil. CNVs of the defensin gene cluster, DEFB4 mRNA, and hBD-2 protein expression were assess...
Source: Biotechnic and Histochemistry - June 18, 2020 Category: Research Authors: Celsi F, Zupin L, Athanasakis E, Orzan E, Grasso DL, Crovella S Tags: Biotech Histochem Source Type: research

Efficacy of modified cell block cytology compared to fine needle aspiration cytology for diagnostic oral cytopathology.
Abstract Cell blocks and fine needle aspirations can be used for cytopathological diagnosis. Conventional fine needle aspiration smears provide limited material for diagnosis. The cell block technique provides more tissue, which improves diagnostic accuracy. We compared a modified cell block cytology to fine needle aspiration for providing optimal preservation of histochemical and immunocytochemical properties. We used 30 fine needle aspirates from oral lesions in two groups: group 1, fine needle aspiration cytology; group 2, cell block cytology. Smears of fine needle aspirates were stained with Papanicolaou. For ...
Source: Biotechnic and Histochemistry - June 18, 2020 Category: Research Authors: Sale MS, Kulkarni VV, Kulkarni PV, Patil CA Tags: Biotech Histochem Source Type: research

Fast acetone tissue processing of human organs provides tissue characteristics equal to conventional processing.
J, Velázquez-Gauna SE, Soto-Domínguez A Abstract Routine preparation of paraffin embedded tissue for histopathological diagnosis, here termed conventional histological technique (CT), whether performed manually or using an automated system, requires approximately 12 h. We developed earlier a rapid acetone dehydration technique (AT) for processing biopsies of nervous tissue that meets requirements for preserving tissue morphology and staining properties, and reduces processing time to 3.3 h. We compared the morphology and staining properties of human organ biopsies including adrenal gland, l...
Source: Biotechnic and Histochemistry - June 12, 2020 Category: Research Authors: García-Garza R, Salas-Treviño D, Soto-Patiño FA, González-Murillo EA, Castelán-Maldonado EE, Saucedo-Cárdenas O, Romero-Díaz VJ, Velázquez-Gauna SE, Soto-Domínguez A Tags: Biotech Histochem Source Type: research

Does melamine exposure during infancy cause rhabdomyolysis?
Abstract We investigated the possible toxic effects of melamine on muscle tissue in rats using biochemical, hematological and histopathological methods. We used three groups of female albino Wistar rats. The first group was given 0.1 ml saline. The second and third groups were given 50 and 75 mg/kg melamine dissolved in 0.1 ml saline, respectively, daily for 21 days. On day 45, all rats were sacrificed, and whole blood and plasma were analyzed for hematologic and biochemical characteristics. Muscle samples were stained with hematoxylin and eosin for histopathological investigation. Other sections we...
Source: Biotechnic and Histochemistry - June 12, 2020 Category: Research Authors: Erisgin Z, Usta M Tags: Biotech Histochem Source Type: research

Use of roselle extracted from Hibiscus sabdariffa for histological staining: a critical review and rational stain formulation.
Abstract Roselle is the common name for a mixture of anthocyanin dyes derived from the plant, Hibiscus sabdariffa. During the past two decades, a sizable, but conflicting, body of literature has supported the use of roselle as a biological stain, and more specifically as a substitute for hematoxylin for staining nuclei selectively. We review the literature and suggest a rational explanation for divergent findings. When used without oxidation and mordanting, roselle is an indiscriminate oversight stain. With appropriate oxidation and mordanting, roselle can be an effective nuclear stain. We propose here use of a st...
Source: Biotechnic and Histochemistry - June 1, 2020 Category: Research Authors: Alshamar HA, Dapson RW Tags: Biotech Histochem Source Type: research

Effects of Juglans regia L. leaf extract supplementation on testicular functions in diabetic rats.
Abstract Testicular dysfunction is a complication of diabetes mellitus (DM). Juglans regia L. (JRL) leaf extract is a source of phenolic compounds that exhibits hypoglycemic and antioxidative properties. We investigated whether JRL leaf extract could inhibit the adverse effects of DM on oxidative stress, testis histology and testosterone hormone production. We used four groups of male rats: control group (non-diabetic) given saline, diabetic group, diabetic + JRL group that received JRL leaf extract, and JRL group (nondiabetic) that received JRL leaf extract only. To evaluate the effects of JRL leaf extract on tes...
Source: Biotechnic and Histochemistry - June 1, 2020 Category: Research Authors: Nasiry D, Khalatbary AR, Ahmadvand H, Talebpour Amiri FB Tags: Biotech Histochem Source Type: research

Isolation and characterization of endothelial progenitor cells from canine bone marrow.
Abstract Endothelial progenitor cells (EPC) are located predominantly in the bone marrow. These cells are useful for treating human vascular diseases; they also are a possible target for restricting blood vessel growth for tumors. Little is known about canine EPC. We investigated a bone marrow EPC isolation method that combines the whole bone marrow culture method and the differential adherent speed method using stillborn canines. MTT proliferation, flow cytometry detection, Dil-ac-LDL uptake, FITC-UEA-1 binding and matrigel assays were used to identify and characterize EPC. We isolated two types of EPC: early EPC...
Source: Biotechnic and Histochemistry - June 1, 2020 Category: Research Authors: Chen C, Dai P, Nan L, Lu R, Wang X, Tian Y, Zhang X, Gao Y, Zheng S, Zhang Y Tags: Biotech Histochem Source Type: research

Micronucleus assay for predicting side effects of radiotherapy for cervical cancer.
Abstract Radiotherapy (RT) is an important treatment for cervical cancer. The quality of life of patients undergoing RT may be compromised during and following treatment by nausea, diarrhea, vomiting, burns, erythema and fistula. Cytokinesis-block micronucleus (CBMN) assays may be useful for predicting adverse effects of RT for cancer. The CBMN test is easy to perform and is reproducible for screening subjects exposed to ionizing radiation. We investigated the use of the frequency of micronuclei (MN) from peripheral blood samples, irradiated in vitro, as a possible biomarker to predict the side effects of RT in pa...
Source: Biotechnic and Histochemistry - May 21, 2020 Category: Research Authors: Borges da Silva E, Brayner Cavalcanti M, Ferreira Da Silva CS, de Salazar E Fernandes T, Azevedo Melo J, Lucena L, Maciel Netto A, Amaral A Tags: Biotech Histochem Source Type: research

Use of selenium to ameliorate doxorubicin induced hepatotoxicity by targeting pro-inflammatory cytokines.
Abstract Doxorubicin (DOX) is a widely used drug for the treatment of cancer,but its clinical use is limited by its liver toxicity. Administering DOX with an antioxidant has become a strategy for preventing the side effects of DOX. Although selenium (Se) is an important trace mineral, data concerning the effect of Se on DOX induced liver tissue are lacking. We investigated the mechanism of DOX hepatotoxicity and the protective effect of different doses of Se on Dox induced liver damage. Female Wistar albino rats were divided into eight equal groups. Se was injected intraperitoneally (i.p.) to rats at doses of 0.5,...
Source: Biotechnic and Histochemistry - May 13, 2020 Category: Research Authors: Cengiz O, Baran M, Balcioglu E, Suna PA, Bilgici P, Goktepe O, Onder GO, Goc R, Yay A Tags: Biotech Histochem Source Type: research

Reduction of hepatorenal and pancreatic damage by Ferula elaeochytris extract in STZ induced diabetic rats.
Abstract The therapeutic potential and antioxidant capacity of Ferula elaeochytris extract (FE) in the liver, kidney and pancreas of rats with diabetes induced by streptozotocin (STZ) was assessed using biochemistry, histopathology and immunohistochemistry. Forty adult Wistar albino male rats were divided randomly into five groups of eight rats each. The normal control (NC) group was untreated. The diabetes control (DC) group was treated with STZ to induce diabetes. The diabetes + acarbose group (DAC) was treated with STZ, then with acarbose daily for 28 days. The diabetes + FE (DFE) group was treated with ST...
Source: Biotechnic and Histochemistry - May 12, 2020 Category: Research Authors: Uyar A, Yaman T, Keles OF, Alkan EE, Demir A, Celik I, Yener Z Tags: Biotech Histochem Source Type: research

Effects of peroxiredoxin 1 on nicotine induced apoptosis in mouse tongue.
Abstract Local action of nicotine on oral mucosa contributes to the pathogenesis of precancerous and cancerous lesions. Nicotine participation in the mechanism of apoptosis in normal mucosa has not been established. Peroxiredoxin 1 (Prx1) is a cellular antioxidant that participates in regulating apoptosis. We investigated expression of Prx1 and proteins in apoptosis-related downstream signaling by mitogen-activated protein kinases (MAPKs) in nicotine-treated tongue tissues of wild-type and Prx1 knockout (Prx1±) mice; we also investigated these processes in mouse embryonic fibroblast (MEF) cells in vitro. Ni...
Source: Biotechnic and Histochemistry - May 4, 2020 Category: Research Authors: Zhang M, Wang C, Chen H, Wang M, Tang X Tags: Biotech Histochem Source Type: research

Antiproliferative and apoptosis inducing effects of the methanolic extract of Centaurea hermannii in human cervical cancer cell line.
z A Abstract We investigated the antiproliferative and apoptosis inducing effects of a methanolic extract of Centaurea hermannii in Vero and HeLa cell lines. Both cell lines also were treated with doxorubicin. Antiproliferative effects were assessed by MTT assay and cell index parameters were determined using the xCELLigence real time cell analysis system (RTCA). The IC50, the half maximal inhibitory concentration, and selectivity index (SI) values for doxorubicin and the C. hermannii extract were determined for both cell lines. At 48 h culture, the C. hermannii extract exhibited a potent cytotoxic effect in ...
Source: Biotechnic and Histochemistry - May 4, 2020 Category: Research Authors: Artun FT, Karagöz A Tags: Biotech Histochem Source Type: research

HNK1 and Sox10 are present during repair of mandibular bone defects.
Abstract Neural crest cells possess characteristics of stem cells including plasticity and ability to differentiate into various cell types. HNK1 and Sox10 are markers of neural crest cell progenitors that have been demonstrated in osteoblasts during osteogenesis of the maxilla and mandible. We investigated the presence of Sox10 and HNK1 during regeneration of mandibular bone defects. Defects were created in mandibles of rats. Samples of these defects were collected at 7, 14 and 28 days post-surgery; bone regeneration was observed during this period. Immunohistochemical analysis revealed expression of HNK1 an...
Source: Biotechnic and Histochemistry - May 4, 2020 Category: Research Authors: Stuepp RT, Modolo F, Trentin AG, Garcez RC, Biz MT Tags: Biotech Histochem Source Type: research

Silibinin and ellagic acid increase the expression of insulin receptor substrate 1 protein in ultraviolet irradiated rat skin.
Abstract Daily exposure to ultraviolet (UV) light induces inflammation and tumorigenesis in the skin. Silibinin and ellagic acid are natural products that exhibit anti-inflammatory and anti-tumorigenic properties. Insulin receptor substrate protein 1 (IRS1) is important for skin homeostasis and physiology, but its activity following UV radiation remains unclear. We investigated the effects of ellagic acid and silibinin on IRS1 expression in ultraviolet A (UVA) and ultraviolet B (UVB) irradiated rat skin. Forty-two female Wistar rats were divided randomly into six groups of seven animals. The dorsal skin of rats wa...
Source: Biotechnic and Histochemistry - April 29, 2020 Category: Research Authors: Gorgisen G, Ozkol H, Tuluce Y, Arslan A, Ecer Y, Keskin S, Kaya Z, Ragbetli MC Tags: Biotech Histochem Source Type: research

Vitamin E effects on developmental disorders in fetuses and cognitive dysfunction in adults following acrylamide treatment during pregnancy.
Abstract We investigated the effects of acrylamide (AA) and vitamin E treatment during pregnancy on brain tissues of fetuses and on adult rats. Pregnant rats were divided into five groups: control, corn oil, vitamin E, AA, vitamin E +AA. The rats administered AA received10 mg/kg/day and those administered vitamin E received 100 mg/kg/day both by via oral gavage for 20 days. On day 20 of pregnancy, half of the pregnant rats were removed by cesarean section in each group. Morphological development parameters were measured in each fetus and histopathological, biochemical and genetic analyses were conducted on th...
Source: Biotechnic and Histochemistry - April 29, 2020 Category: Research Authors: Erdemli Z, Erdemli ME, Turkoz Y, Yigitcan B, Aladag MA, Cigremis Y, Cırık RH, Altinoz E, Bag HG Tags: Biotech Histochem Source Type: research

Quirks of dye nomenclature. 15. Geranine - a simple name, with a less than straight forward identity.
Abstract Geranines were manufactured initially as textile dyes; they were made by coupling diazotized aromatic amines with sulfonated 1-naphthols. Most commonly encountered was geranine G, which for more than fifty years was thought to be derived from 1-naphthol monosulfonic acid, but later was considered to be derived from a 1-naphthol disulfonic acid. Currently, geranine G is thought to be a mixture of two isomers derived from 1-naphthol disulfonic acids. This species and others are described here by chemical structure and by other reference names and numbers where available. The occasional uses of geranines as ...
Source: Biotechnic and Histochemistry - April 28, 2020 Category: Research Authors: Cooksey CJ Tags: Biotech Histochem Source Type: research

Certification procedures used by the Biological Stain Commission for eriochrome cyanine R (C.I. 43820, Mordant blue 3).
Abstract Eriochrome cyanine R (C.I. 43820, Mordant blue 3), also known as chromoxane cyanine R and solochrome cyanine R, has been used as a biological stain since 1957. In conjunction with ferric ions, it provides selective blue coloration of the nuclei of cells in methods procedurally similar to commonly used progressive or regressive hemalum (aluminum-hematoxylin) stains. Eriochrome cyanine R also is used to stain the myelin sheaths of axons in nerve tissue; the results are visually similar to those in sections stained with luxol fast blue MBS (C.I. 74180, solvent blue 38) with selective blue coloration of myeli...
Source: Biotechnic and Histochemistry - April 23, 2020 Category: Research Authors: Fagan C, Kiernan JA Tags: Biotech Histochem Source Type: research

Panaxynol from Saposhnikovia diviaricata exhibits a hepatoprotective effect against lipopolysaccharide + D-Gal N induced acute liver injury by inhibiting Nf- κB/IκB-α and activating Nrf2/HO-1 signaling pathways.
Panaxynol from Saposhnikovia diviaricata exhibits a hepatoprotective effect against lipopolysaccharide + D-Gal N induced acute liver injury by inhibiting Nf-κB/IκB-α and activating Nrf2/HO-1 signaling pathways. Biotech Histochem. 2020 Apr 16;:1-9 Authors: Sun X, Zhang T, Zhao Y, Cai E, Zhu H, Liu S Abstract We investigated the mechanism of action of panaxynol (PAL) extract from the root of Saposhnikovia diviaricata (Turcz.) Schischk for treating acute liver injury caused by lipopolysaccharide (LPS) and D-galactosamine (D-Gal N) in mice. A mouse model of acute liver failure induced by...
Source: Biotechnic and Histochemistry - April 16, 2020 Category: Research Authors: Sun X, Zhang T, Zhao Y, Cai E, Zhu H, Liu S Tags: Biotech Histochem Source Type: research

Association of extracellular matrix microarchitecture and three-dimensional collective invasion of cancer cells.
Abstract As much as 90% of cancer associated mortality follows metastasis of a primary tumor. Circulating tumor cells (CTCs) and CTC clusters are important for metastasis. Compared to CTCs, CTC clusters formed by collective invasion exhibit a 23-50 fold increase in metastatic potential, but the factors that influence collective invasion are largely unknown. Using well defined three-dimensional matrices and different extracellular matrix (ECM) concentrations, we found that cancer cells were more prone to collective invasion at low ECM concentration. Moreover, despite variation of biological factors, changes in ECM ...
Source: Biotechnic and Histochemistry - April 15, 2020 Category: Research Authors: Quan Q, Wang X, Lu C, Ma W, Han J, Xia G, Yang G, Wang C Tags: Biotech Histochem Source Type: research

Is low level laser therapy or ozone therapy more effective for bone healing? Understanding the mechanisms of HIF-1 α, RANKL and OPG.
Is low level laser therapy or ozone therapy more effective for bone healing? Understanding the mechanisms of HIF-1α, RANKL and OPG. Biotech Histochem. 2020 Apr 15;:1-8 Authors: Bayer Alinca S, Sağlam E, Zengin Celik T, Hacisalihoglu P, Doğan MA Abstract Periodontitis is a common chronic infection of dental tissues. Ozone therapy (OT) and low level laser therapy (LLLT) are useful treatments for periodontitis. We investigated the effects of OT and LLLT on periodontal disease-induced bone destruction in rats with experimentally induced periodontitis (EP). We used 30 male Wistar rats divided into ...
Source: Biotechnic and Histochemistry - April 15, 2020 Category: Research Authors: Bayer Alinca S, Sağlam E, Zengin Celik T, Hacisalihoglu P, Doğan MA Tags: Biotech Histochem Source Type: research

Gemfibrozil attenuates doxorubicin induced toxicity in renal tissues of male rats by reducing the oxidative insult and inflammation.
Abstract Nephrotoxicity is a significant side effect of doxorubicin (DXN) treatment. We investigated the protective effect of gemfibrozil (GEM) co-administration with DXN on DXN induced nephrotoxicity. We divided 28 male Wistar rats into four groups of seven. Group 1 received normal saline for 2 weeks. Group 2 received 15 mg/kg DXN for 2 weeks. Group 3 received DXN + GEM for 2 weeks. Group 4 received GEM for 2 weeks. On day 15 of the experiment, blood samples were collected, animals were sacrificed and kidneys were excised for biochemical and histological evaluation. We measured serum creatinin...
Source: Biotechnic and Histochemistry - April 3, 2020 Category: Research Authors: Hosseinzadeh A, Goudarzi M, Fatemi I, Khodayar MJ, Mehrzadi S, Khalili HR, Karimi MA Tags: Biotech Histochem Source Type: research

Role of ADAMTS-13 and nNOS expression in neuropathogenesis of listeric encephalitis of small ruminants.
Abstract We investigated the expression of A disintegrin and metalloprotease with thrombospondin type I repeats-13 (ADAMTS-13) in the central nervous system (CNS), because it is related to blood-brain barrier (BBB) permeability. We also investigated 8-OHdG, caspase-3 and neuronal nitric oxide synthase (nNOS) expression for the cytotoxic effects of oxidative stress (OS) and nNOS, and their relation to apoptosis. We also investigated the neuroimmunopathology caused by L. monocytogenes. Brain tissues were obtained from 10 lambs and 10 kids with listeric meningoencephalitis, and healthy brain tissue from six lambs and...
Source: Biotechnic and Histochemistry - April 2, 2020 Category: Research Authors: Karayigit MO, Dincel GC Tags: Biotech Histochem Source Type: research

Quantification of viable granulosa cells in murine ovarian follicles.
Abstract Ovarian follicle growth and oocyte maturation depend on the viability of granulosa cells (GC). We quantified GC in whole mouse follicles. Single follicles were isolated from adult mouse ovaries and stained with DAPI or Live-Dead stain before fixation. An objective image analysis protocol for counting fluorescent labeled GC was developed that used Image J software to measure GC cytoplasmic and nuclear areas. These data were compared to the number of GC obtained by disaggregating 96 follicles with enzymes to produce a suspension of GC, which then was stained with trypan blue and assessed using a hemocytomet...
Source: Biotechnic and Histochemistry - March 25, 2020 Category: Research Authors: Asaduzzaman M, Rodgers RJ, Young FM Tags: Biotech Histochem Source Type: research

Thymoquinone protection against 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin induced nephrotoxicity in rats.
Abstract We investigated the effects of thymoquinone (TQ) on kidney tissues of Wistar rats with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induced nephrotoxicity. We used 50 rats divided into five groups; control, corn oil, TCDD, TQ, TCDD + TQ. We found that malondialdehyde (MDA), total oxidant status (TOS), blood urea nitrogen (BUN), creatinine, interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) levels in the TCDD treated group increased significantly compared to the other groups, while reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant status (TAS) levels d...
Source: Biotechnic and Histochemistry - March 24, 2020 Category: Research Authors: Erdemli ME, Yigitcan B, Erdemli Z, Gul M, Bag HG, Gul S Tags: Biotech Histochem Source Type: research

Anti-leukemic activity of Satureja bachtiarica occurs by apoptosis in human cells.
Abstract Failure of apoptosis contributes to leukemia progression. We investigated extracts of a native Iranian plant, Satureja bachtiarica, for possible anti-leukemia activity by induction of apoptosis and changes to the cell cycle. Growth inhibition caused by aqueous, butanol, dichloromethane and hexane extracts of S. bachtiarica on K562 and Jurkat leukemia cells was assessed using a colorimetric assay. Extracts were analyzed for induction of apoptosis and cell cycle arrest using flow cytometry and measurement of caspase-3 activity. Dichloromethane and hexane extracts inhibited leukemia cell proliferation in a d...
Source: Biotechnic and Histochemistry - March 17, 2020 Category: Research Authors: Asadipour M, Malek-Hosseini S, Amirghofran Z Tags: Biotech Histochem Source Type: research

Misoprostol ameliorates doxorubicin induced cardiac damage by decreasing oxidative stress and apoptosis in rats.
Abstract We investigated the potential cardioprotective effects of misoprostol (MP) on doxorubicin (DOX) induced cardiac damage using histologic and biochemical assessment of rat heart. We used 21 male rats divided randomly into three groups: group 1, control; group 2, DOX; group 3, DOX + MP. The control group was given 0.5 ml 0.9% NaCl intraperitoneally (i.p.) and 1 ml 0.9% NaCl orally for 6 days. DOX was administered as a single dose of 20 mg/kg i.p. on day 3. MP was administered orally for 6 days. We found that treatment with MP decreased significantly serum cardiac troponin-I, brain natriuretic peptide levels,...
Source: Biotechnic and Histochemistry - March 17, 2020 Category: Research Authors: Bilgic S, Ozgocmen M, Ozer MK, Asci H Tags: Biotech Histochem Source Type: research

Appearance and differentiation of NADPH-d-positive neurons in rat prefrontal cortex following exposure to retinoic acid.
Matéffy S, Kluchová D Abstract Retinoic acid (RA) is a biologically active form of vitamin A. Teratogenicity has been observed in pregnant mammals exposed to high doses of vitamin A. We investigated the distribution of nitrergic neurons in rat prefrontal cortex (PFC) at developmental stages 7 days to young adulthood under physiological conditions and after prenatal application of all trans-RA. The neurons were studied histochemically using NADPH-diaphorase, which stains neurons dark blue. We found that nitrergic neurons differentiate rapidly and reach structural maturity by the end of the second week...
Source: Biotechnic and Histochemistry - March 3, 2020 Category: Research Authors: Hvizdošová N, Ihnátová L, Bona M, Matéffy S, Kluchová D Tags: Biotech Histochem Source Type: research