Germ cell differentiation of mouse embryonic stem cells can be influenced by the culture medium.
Abstract Although embryonic stem (ES) cells can differentiate into germ cells, little is known about the influence of culture media on this process. We investigated the effect of two culture media on the capacity of ES cells to differentiate into germ cells using embryoid body (EB) and monolayer culture protocols. Germ cell differentiation was induced in mouse ES cells under four experimental conditions: EB/Dulbecco's modified Eagle's medium (EB/DMEM), EB/knockout Dulbecco's modified Eagle's medium (EB/KO-DMEM), monolayer/Dulbecco's modified Eagle's medium (monolayer/DMEM), and monolayer/knockout Dulbecco's modifi...
Source: Biotechnic and Histochemistry - October 16, 2019 Category: Research Authors: Bahmanpour S, Talaei Khozani T, Soleimani A, Zareifard N Tags: Biotech Histochem Source Type: research

Quirks of dye nomenclature. 13. Biebrich scarlet.
Abstract Biebrich scarlet was the first commercial bis-azo dye when it appeared on the market in 1879 in Biebrich on Rhine, Germany. The dye's early history is recounted here with details of the manufacturing process. The possibility that the dye exists in a keto form rather than an enol form is discussed. Application as a textile dye was soon followed by use as a biological stain and for medical applications. Efforts to decolorize the dye to reduce environmental impact are described. PMID: 31592687 [PubMed - as supplied by publisher] (Source: Biotechnic and Histochemistry)
Source: Biotechnic and Histochemistry - October 8, 2019 Category: Research Authors: Cooksey CJ Tags: Biotech Histochem Source Type: research

Comparison of the proteome patterns of adipose-derived stem cells with those treated with selegiline using a two dimensional gel electrophoresis analysis.
Abstract Adipose derived stem cells (ADSCs) are multipotent and can transdifferentiate into neural stem cells. We investigated the transdifferentiation of ADSCs to neural phenotype (NP) cells using selegiline and two-dimensional electrophoresis (2-DE). The perinephric and inguinal fat of rats was collected and used to isolate ADSCs that were characterized by immunophenotyping using flow cytometry. The ADSCs were differentiated into osteogenic and lipogenic cells. The NP cells were generated using 10-9 mM selegiline and characterized by immunocytochemical staining of nestin and neurofilament 68 (NF-68), and by qRT-...
Source: Biotechnic and Histochemistry - October 7, 2019 Category: Research Authors: Mardani M, Tiraihi T, Bathaie SZ, Mirnajafi-Zadeh J Tags: Biotech Histochem Source Type: research

Can novel adipokines, asprosin and meteorin-like, be biomarkers for malignant mesothelioma?
Abstract Malignant mesothelioma (MM) is a rare tumor of serous surfaces that has a poor prognosis. Cancer is a multistage process by which cells undergo metabolic and behavioral changes that cause excessive and untimely proliferation. Asprosin (ASP) and meteorin-like (METRNL) are two peptides associated with glucose and energy metabolism. We used immunohistochemistry to investigate whether these peptides could be biomarkers for diagnosis and treatment of MM. We reviewed 30 cases of MM and 30 cases of reactive mesothelial hyperplasia (RMH); we used the cases with RMH as control group. The specimens were examined us...
Source: Biotechnic and Histochemistry - October 1, 2019 Category: Research Authors: Kocaman N, Artaş G Tags: Biotech Histochem Source Type: research

Fluoxetine ameliorates adult hippocampal injury in rats after early maternal separation. A biochemical, histological and immunohistochemical study.
Abstract The mother-infant relation is important for brain development and maturation. To investigate hippocampus changes, we used 18 male rat pups from three dams. Pups were divided into a maternal care (control) group, a maternal separation (MS) group and a MS plus fluoxetine (MS + Fl) treated group. On postnatal day 22, pups were weaned and their serum corticosterone level measured. At 2 months, the hippocampus was removed and processed for histological, immunohistochemical and ultrastructural study. MS caused significant elevation of serum corticosterone level and a significant decrease in the thickness of the...
Source: Biotechnic and Histochemistry - October 1, 2019 Category: Research Authors: Arafat EA, Shabaan DA Tags: Biotech Histochem Source Type: research

Effects of paclitaxel and resveratrol on blood characteristics in rabbits.
HH Abstract We investigated the adverse effects of paclitaxel (PAC) on blood characteristics including the percentage of alpha naphthyl acetate esterase (ANAE)-positive peripheral blood lymphocytes, white cell count, number of neutrophil nuclear lobe and, erythrocyte diameter, and the protective effects of resveratrol (RES) on these characteristics. Male New Zealand rabbits were assigned to four groups. The control group (C) was given 40 ml normal saline, the PAC group was given 5 mg/kg PAC in 40 ml normal saline, the RES group was given 4 mg/kg RES in 40 ml normal saline, and the PAC + RES group was given 5 mg/k...
Source: Biotechnic and Histochemistry - October 1, 2019 Category: Research Authors: Kaya S, Dönmez HH Tags: Biotech Histochem Source Type: research

Histopathological and biochemical assessment of a novel diagnostic method for ovarian torsion.
Abstract Ovarian torsion is agynecologic emergency that affects females of all ages. Early diagnosis is important to preserve ovarian function. The false positive rate for sonographic diagnosis of ovarian torsion is 50%; therefore, a new real-time approach is required to improve diagnostic accuracy. We investigated diffuse reflectance spectroscopy for diagnosing ovarian torsion. Spectroscopic measurements were performed in vivo prior to, during and after detorsion. After bilateral oophorectomy, hemoxygenase and myeloperoxidase enzyme activity in ovarian tissue was evaluated and the tissues were examined for pathol...
Source: Biotechnic and Histochemistry - October 1, 2019 Category: Research Authors: Ozekinci M, Kucuksayan E, Erdogan G, Aslan M, Pestereli E, Canpolat M, Sircan-Kucuksayan A Tags: Biotech Histochem Source Type: research

Interleukin-23 is constitutively expressed in the human annulus in vivo and in vitro, and is up-regulated in vitro by TNF- α.
Interleukin-23 is constitutively expressed in the human annulus in vivo and in vitro, and is up-regulated in vitro by TNF-α. Biotech Histochem. 2019 Sep 20;:1-6 Authors: Gruber HE, Marrero E, Cox M, Hanley E Abstract Interleukin-23 (IL-23, IL-23p19) is a proinflammatory cytokine in the IL-12-related family. Although inflammatory cells in herniated discs have been shown to contain IL-23, little is known about the presence and role of IL-23 in human disc cells. We analyzed disc specimens for IL-23 localization using immunohistochemistry in control, herniated and non-herniated discs from which annu...
Source: Biotechnic and Histochemistry - September 20, 2019 Category: Research Authors: Gruber HE, Marrero E, Cox M, Hanley E Tags: Biotech Histochem Source Type: research

Royal jelly increased map-2 expression in hippocampal neurons of hypothyroid rats: an immunohistochemical study.
Elmas Ç Abstract We investigated microtubule-associated protein 2 (MAP-2) immunoreactivity of hippocampal neurons and the potential role of royal jelly (RJ) in regulating MAP-2 during experimental hypothyroidism (HT). Thirty adult female Wistar albino rats were randomized into five groups: the control group was untreated, the sham control group was treated with 10 mg/kg 0.9% sterile saline injected intraperitoneally (i.p.), The RJ group was treated with 100 mg/kg RJ by oral gavage, the HT group was treated with 10 mg/kg propylthiouracil (PTU) i.p. to induce experimental hypothyroidism, and the HT + RJ grou...
Source: Biotechnic and Histochemistry - September 11, 2019 Category: Research Authors: Seymen CM, Çakır Gündoğdu A, Bulut DI, Yılmaz Demirtaş C, Elmas Ç Tags: Biotech Histochem Source Type: research

Immunohistochemistry for diagnosis and prognosis of breast cancer: a review.
Abstract Breast cancer is the most prevalent malignant tumor and main oncologic cause of mortality in women. Although most diagnosis of breast pathology is accomplished using hematoxylin and eosin stained sections, some cases require immunohistochemistry for proper evaluation. We investigated the latter cases including distinctions between ductal and lobular carcinoma, in situ and invasive carcinoma, typical ductal hyperplasia and atypical ductal hyperplasia/ductal carcinoma in situ, papillary and spindle cell lesion assessment, metastasis evaluation, and assessment of prognostic and therapy markers. E-cadherin is...
Source: Biotechnic and Histochemistry - September 10, 2019 Category: Research Authors: Bonacho T, Rodrigues F, Liberal J Tags: Biotech Histochem Source Type: research

Thymoquinone protection from amikacin induced renal injury in rats.
Abstract We investigated whether thymoquinone (TQ) exerts a beneficial effect on renal injury due to amikacin (AK) administration in rats. To generate kidney damage with AK, a single 1.2 g/kg dose of AK was administered intraperitoneally. TQ was administered orally to the AK treated group at a dose of 40 mg/kg for five days. At the end of the experiment, rats were sacrificed and blood samples were used to measure blood urea nitrogen (BUN) and creatinine (Cr) levels. Kidney samples were taken to measure the oxidative stress biomarker, malondialdehyde (MDA), and expression of the antioxidant enzymes, superoxide...
Source: Biotechnic and Histochemistry - September 10, 2019 Category: Research Authors: Ozer MK, Bilgic S, Armagan I, Savran M Tags: Biotech Histochem Source Type: research

1p36 is a chromosomal site of genomic instability in cervical intraepithelial neoplasia.
aacute;vila H, Fernández JL, Gosálvez J Abstract We investigated the association between progressive stages of cervical neoplasia and DNA damage in 1p36 DNA sequences of chromosome 1 in cervical epithelium using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). We used a hospital based unmatched case control study of 29 women that were grouped according to disease stage and selected according to histological diagnosis: 10 with low grade squamous intraepithelial lesions (LG-SILs), 10 with high grade SILs (HG-SILs) and nine with no cervical lesions; the 1pter sequence was used as in...
Source: Biotechnic and Histochemistry - September 10, 2019 Category: Research Authors: Cortés-Gutiérrez EI, García-Vielma C, Dávila-Rodríguez MI, Sánchez-Dávila H, Fernández JL, Gosálvez J Tags: Biotech Histochem Source Type: research

Effects of co-administered melatonin, fructose and bisphenol A (BPA) on rat epididymis and sperm characteristics.
Ömeroğlu S Abstract Consumption of fructose-rich food and exposure to endocrine disrupting chemicals continue to increase. High fructose consumption is associated with increased incidence of dyslipidemia, hypertension, hyperuricemia and insulin resistance. Bisphenol A (BPA) is an environmental contaminant that exhibits estrogen-like activity; it impairs reproductive organs, sperm production, spermatogenesis and fertility. We investigated the possible ameliorative effects of melatonin on rat epididymis and sperm characteristics following exposure to fructose and BPA. We used 42 adult male Sprague-Dawley rats...
Source: Biotechnic and Histochemistry - September 4, 2019 Category: Research Authors: Akarca-Dizakar SÖ, Erdoğan D, Peker T, Coşkun Akçay N, Türkoğlu I, Eşmekaya MA, Ömeroğlu S Tags: Biotech Histochem Source Type: research

The protective effect of misoprostol against doxorubicin induced liver injury.
Abstract We investigated the potential hepatoprotective effects of misoprostol (MP) on doxorubicin (DOX) induced liver injury in rats using histology and biochemistry. We used 21 male Sprague-Dawley rats divided randomly into three groups: group 1, control; group 2, DOX; group 3, DOX + MP. The control group was injected intraperitoneally (i.p.) with 0.5 ml 0.9% w/v NaCl and given 1 ml 0.9% NaCl orally for 6 days. DOX was administered i.p. as a single dose of 20 mg/kg. MP, 0.2 mg/kg, was given orally for 6 days. Treatment with MP increased high density lipoprotein cholesterol levels and decreased serum al...
Source: Biotechnic and Histochemistry - September 3, 2019 Category: Research Authors: Bilgic S, Ozgocmen M Tags: Biotech Histochem Source Type: research

Evaluation of oxidative DNA damage in pigeon erythrocytes using DNA breakage detection-fluorescence in situ hybridization (DBD-FISH).
ras JP, González-Ramírez EG Abstract DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) enables detection and quantification of DNA breakage in the entire genome or within specific DNA sequences in single cells. We used this method to visualize and evaluate DNA damage in pigeon erythrocytes that were induced by elevated temperature and hydrogen peroxide. We also examined morphological changes in the cell nuclei. DBD-FISH demonstrated a significant increase of DNA damage in a temperature dependent manner, which resulted in nuclear abnormalities associated with apoptotic cells. These ...
Source: Biotechnic and Histochemistry - August 23, 2019 Category: Research Authors: Cortés-Gutiérrez EI, García-Salas JA, Dávila-Rodríguez MI, Ceyca-Contreras JP, González-Ramírez EG Tags: Biotech Histochem Source Type: research

Effects of iloprost and sildenafil treatment on elabela, apelin-13, nitric oxide, and total antioxidant and total oxidant status in experimental enzyme-positive acute coronary syndrome in rats.
Abstract Despite significant advances in medicine, mortality due to cardiovascular disease is not yet preventable. We investigated the amounts of elabela (ELA) and apelin, synthesized by cardiomyocytes, and changes of these compounds in cardiac tissue and circulation after administration of iloprost (ILO) and sildenafil (SIL) in rats with induced myocardial ischemia (MI). We also investigated a connection with circulating troponin-I, creatine kinase (CK), creatine kinase-myocardial band (CK-MB) and nitric oxide (NO), and total anti-oxidant (TAS)/total oxidant status (TOS). We established eight study groups of five...
Source: Biotechnic and Histochemistry - August 20, 2019 Category: Research Authors: Aydin S, Kuloglu T, Aydin Y, Yalcin MH, Ugur K, Albayrak S, Aksoy A, Sahin I, Dagli AF, Akkoc RF, Aydin S Tags: Biotech Histochem Source Type: research

Effects of misoprostol treatment on doxorubicin induced renal injury in rats.
Abstract We investigated the potential nephroprotective effects of misoprostol (MP) on doxorubicin (DOX) induced renal injury using histologic and biochemical assessment of rat kidneys. We used 21 male rats divided into three groups: group 1, control; group 2, DOX; group 3, DOX + MP. The control group was given 0.5 ml 0.9% NaCl and 1 ml 0.9% NaCl orally for 6 days. DOX was administered as a single dose of 20 mg/kg on day 3. MP was administered orally for 6 days. We found that treatment with MP decreased serum creatinine and blood urea nitrogen levels significantly. DOX increased the malond...
Source: Biotechnic and Histochemistry - August 20, 2019 Category: Research Authors: Bilgic S, Armagan I Tags: Biotech Histochem Source Type: research

The Effects of folic acid and topiramate on peripheral nerve regeneration.
Abstract Peripheral nerve injury creates unusual sensitivity and pathological spontaneous activity in neurons that are described as ectopic discharge. Voltage dependent Na channels are responsible for ectopic discharge. Topiramate (TOP) inhibits voltage-gated sodium channels by blocking both the amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)/kainate receptor and folic acid (FA) activity in neurotransmitter synthesis reactions. FA and TOP are anti-apoptotic agents by both phosphorylated-Akt (p-Akt) signaling activation and anti-inflammatory effects at the injury site. We investigated the effects of FA a...
Source: Biotechnic and Histochemistry - August 19, 2019 Category: Research Authors: Sağır D, Kuruoğlu E, Onger ME, Yarar E Tags: Biotech Histochem Source Type: research

Orally administered resveratrol enhances the therapeutic effect of autologous transplanted adipose-derived stem cells on rats with diabetic hepatopathy.
Abstract Stem cell therapy is a promising treatment for hepatopathy due to diabetes mellitus (DM); oral resveratrol treatment exhibits protective effects. We investigated whether protective effects could be produced in liver of diabetic rats receiving autologous adipose-derived stem cell transplantation (ADSC) plus oral resveratrol administration. Male rats were divided into four groups: sham group; streptozotocin induced DM group; DM + ADSC group, in which DM rats were treated with 106 stem cells/rat; and DM + R + ADSC group, in which DM rats were treated with ADSC and oral resveratrol. The DM group exhibite...
Source: Biotechnic and Histochemistry - August 19, 2019 Category: Research Authors: Chen TS, Lai CH, Shen CY, Pai PY, Chen RJ, PadmaViswanadha V, Yang CK, Chen MC, Lin YM, Huang CY Tags: Biotech Histochem Source Type: research

In tube immunocytochemistry for fluorescence-activated cell sorting that prevents RNA degradation in sorted cells.
Abstract Fluorescence-activated cell sorting (FACS) is a powerful tool for analyzing stem cells. When using fixed cells, however, it is sometimes difficult to analyze RNA extracted from sorted cells due to RNA degradation. We established a protocol for immunocytochemistry before FACS to prevent RNA degradation. Cells were fixed with a methanol-based fixative (UM-Fix), then subjected to immunocytochemistry. The addition of RNase inhibitor and dithiothreitol (DTT) to some buffers used for immunocytochemistry increased RNA integrity after cell recovery. We found increased copy numbers of mRNA in recovered cells using...
Source: Biotechnic and Histochemistry - August 19, 2019 Category: Research Authors: Fukano H, Takano T, Fujimoto Y, Nakatani R, Watanabe M, Hidaka Y, Shimomura I Tags: Biotech Histochem Source Type: research

Salivary and serum biomarkers of inflammation in a man with metastatic medullary thyroid carcinoma and hyperreactive gingiva: a fourteen year odyssey.
;n ES Abstract A peripheral (gingival) fibroma, a gingival cyst and hyperplastic gingivitis occurred simultaneously in a man with metastatic medullary thyroid carcinoma (MCT). The gingival growths and hyperplasia appeared to be related to poor oral hygiene rather than to the MTC. Despite the patient's improved oral hygiene, the hyperplastic gingivitis and peripheral fibroma recurred, and a new peripheral fibroma and gingival cyst developed, which prompted reconsideration of a link with the MTC. MTC cells secrete calcitonin (CT), procalcitonin (ProCT) and growth factors; the patient's serum CT and ProCT were severa...
Source: Biotechnic and Histochemistry - August 19, 2019 Category: Research Authors: Redman RS, Bayley NC, Nylén ES Tags: Biotech Histochem Source Type: research

The relation of oxidative stress and apoptosis to histopathologic alterations in the lungs as a result of global cerebral ischemia.
Abstract Heart attack and oxygen deficiency may cause necrosis in the brain and other tissues. We investigated the histopathological effects of nitric oxide (NO) on ischemia/reperfusion in lung and hippocampus using a rat brain bilateral occlusion ischemia model. Male rats were assigned to sham (SH), ischemic preconditioning (PC), global ischemia (GI) and ischemic reperfusion (IR) groups. Before ischemia was induced, blood was drawn to induce hypovolemic hypotension and for blood gas testing. After sacrifice, samples of hippocampus were harvested. Sections were examined using hematoxylin and eosin (H & E) stai...
Source: Biotechnic and Histochemistry - August 2, 2019 Category: Research Authors: Yeşil H, Tuğlu I Tags: Biotech Histochem Source Type: research

Effect of tamoxifen on the Notch signaling pathway in ovarian follicles of mice.
ED Abstract We investigated the effect of tamoxifen (TAM) treatment on the Notch signaling pathway in mouse ovary. Mice were randomly divided into four groups. Control group A animals were untreated. Control group B animals were treated with the vehicle only. Animals of the 0.5 TAM group received 0.5 mg/day TAM. Animals of the 1.5 TAM group received 1.5 mg/day of TAM. TAM was injected subcutaneously for 5 days. Body weights were measured at the start and end of the experiment. Sections were stained using Crossman's modified trichrome to examine general ovarian structure. Other sections were immunostained to ...
Source: Biotechnic and Histochemistry - July 15, 2019 Category: Research Authors: Zık B, Kurnaz H, Güler S, Asmaz ED Tags: Biotech Histochem Source Type: research

Determination of genotoxic and antigenotoxic effects of wild-grown Reishi mushroom (Ganoderma lucidum) using the hen's egg test for analysis of micronucleus induction.
zparlak H Abstract The micronucleus (MN) technique is commonly used for genotoxicity testing. The hen's egg test (HET) for analysis of MN induction (HET-MN) is an inexpensive, rapid and simple genotoxicity assay that is compatible with animal protection and ethical considerations. Ganoderma lucidum (Curtis) P. Karst is known also as reishi mushroom and mushroom of immortality. It has long been used to treat disorders including fungal infections, influenza, common cold, hepatitis, diabetes, high cholesterol and cancer in many countries including China and Japan. G. lucidum strengthens the immune system and reduces ...
Source: Biotechnic and Histochemistry - July 8, 2019 Category: Research Authors: Çelik B, Özparlak H Tags: Biotech Histochem Source Type: research

The effects of resveratrol administration on lipid oxidation in experimental renal ischemia-reperfusion injury in rats.
Abstract We investigated how resveratrol affects lipid oxidation during experimental renal ischemia-reperfusion injury in rats. We used 48 adult male rats assigned to five groups: group 1, control; group 2, renal ischemia; group 3, renal ischemia + reperfusion; group 4, resveratrol + renal ischemia; group 5, resveratrol + renal ischemia + reperfusion. Plasma and renal tissue malondialdehyde (MDA), and erythrocyte and renal tissue glutathione (GSH) levels were measured and histologic changes in the renal tissue were examined. Ischemia-reperfusion affected the MDA-GSH balance adversely and caused histopathological c...
Source: Biotechnic and Histochemistry - July 4, 2019 Category: Research Authors: Baltaci AK, Gokbudak H, Baltaci SB, Mogulkoc R, Avunduk MC Tags: Biotech Histochem Source Type: research

Systematic identification of lncRNAs and circRNAs-associated ceRNA networks in human lumbar disc degeneration.
Abstract Lumbar disc degeneration (LDD) is a common cause of low back and neck pain. The molecular mechanisms underlying LDD, however, are unclear. Noncoding RNAs have been reported to participate in human diseases. We investigated a series of public datasets (GSE67566, GSE56081 and GSE63492) and identified 568 mRNAs, 55 microRNAs (miRNAs), 765 long noncoding RNAs (lncRNAs), and 586 circular RNAs (circRNAs) that were expressed differently in LDD than in normal discs. We constructed lncRNAs and circRNAs regulated competing endogenous RNAs (ceRNA) networks in LDD. Four lncRNAs, DANCR, CASK-AS1, SCARNA2, and LINC0063...
Source: Biotechnic and Histochemistry - July 4, 2019 Category: Research Authors: Zhang YH, Song J, Shen L, Shao J Tags: Biotech Histochem Source Type: research

Tumor apelin immunoreactivity is correlated with body mass index in ovarian high grade serous carcinoma.
Abstract Ovarian cancer has a high mortality rate. Serous carcinoma is the most common subtype and can be detected by distant or lymph node metastasis in advanced stages. Apelin, an adipokine associated with obesity, and its receptor, APJ, participate in lymphatic invasion. Angiogenesis also can affect lymph node involvement in serous ovarian carcinomas. We investigated apelin/APJ receptor immunoreactivity in stages III and IV ovarian cancer with or without lymph node involvement and correlated the results with body mass index (BMI) to determine whether the potential relation of the two affects the outcome of the ...
Source: Biotechnic and Histochemistry - July 2, 2019 Category: Research Authors: Unal I, Khiavi IR, Tasar GE, Goksuluk D, Boyraz G, Ozgul N, Usubutun A, Yuruker S, Zeybek ND Tags: Biotech Histochem Source Type: research

The effect of exercise on anxiety- and depression-like behavior of aged rats.
Abstract We investigated the effects of exercise in multiple sessions on anxiety- and depression-like behavior during aging, and the role of serotonin and serotonin 1A receptors in this process. Both 24-month-old (aged) and 6-month-old (adult) female rats were divided into five groups; aged control, adult control, aged + serotonin re-uptake inhibitors (SSRIs), aged + exercise, and aged + SSRIs + exercise. After exercise, all groups were evaluated using the open field arena, elevated plus maze and forced swim tests. We assessed serum corticosterone levels; number of amygdala, hippocampus and prefrontal cortex cells...
Source: Biotechnic and Histochemistry - July 2, 2019 Category: Research Authors: Gokdemir O, Cetinkaya C, Gumus H, Aksu I, Kiray M, Ates M, Kiray A, Baykara B, Baykara B, Sisman AR, Uysal N Tags: Biotech Histochem Source Type: research

Immunohistochemical profile of CD markers in experimental neural tube defect.
Abstract Neural tube defects (NTDs) are the second most common birth defects worldwide. Stem cells play a critical role in the mechanisms underlying NTDs. We established an experimental NTD model in rats using retinoic acid (RA). We used mesenchymal and hemopoietic stem cell markers to determine their distribution in the mesenchyme in and around the neuroepithelium during the embryonic and fetal periods in both cranial and caudal regions. Adult female rats were given RA on days 5 and 10 of gestation and olive oil was administered to the control group. On days 10.5 and 15.5, embryos in the experimental and control ...
Source: Biotechnic and Histochemistry - June 11, 2019 Category: Research Authors: Sahin Inan ZD, Unver Saraydin S Tags: Biotech Histochem Source Type: research

ALK5 is essential for tooth germ differentiation during tooth development.
Abstract The TGFβ superfamily of proteins participates in tooth development. TGFβ1 and TGFβ3 regulate odontoblast differentiation and dentin extracellular matrix synthesis. Although the expression of TGFβ family member ligands is well-characterized during mammalian tooth development, less is known about the TGFβ receptor, which is a heteromeric complex consisting of a type I and type II receptors. The molecular mechanism of ALK5 (TGFβR1) in the dental mesenchyme is not clear. We investigated the role of ALK5 in tooth germ mesenchymal cells (TGMCs) from the lower first molar tooth germ...
Source: Biotechnic and Histochemistry - May 30, 2019 Category: Research Authors: Guo W, Fan Z, Wang S, Du J Tags: Biotech Histochem Source Type: research

A histological, immunohistochemical and biochemical study of the effects of pomegranate peel extracts on gibberellic acid induced oxidative stress in adult rat testes.
Abstract Gibberellins are commonly used plant growth regulators that exhibit deleterious effects on various animal tissues. We investigated the histological, immunohistochemical and biochemical effects of gibberellic acid (GA3) on rat testes as well as the possible protective role of pomegranate peel extract (PPE). We used 28 adult male rats divided into control, PPE treated, GS3 treated and GA3 + PPE treated groups. Testis specimens were analyzed for superoxide dismutase (SOD) and catalase (CAT) activity, and examined histologically. We also investigated the androgen receptor using immunohistochemistry. The ...
Source: Biotechnic and Histochemistry - May 30, 2019 Category: Research Authors: Khalaf HA, Arafat EA, Ghoneim FM Tags: Biotech Histochem Source Type: research

Dose dependent effects of oxytocin on cognitive defects and anxiety disorders in adult rats following acute infantile maternal deprivation stress.
Abstract Maternal deprivation at an early age is a powerful stressor that causes permanent alterations in cognitive and behavioral functions during the later stages of life. We investigated the effects of oxytocin on cognitive defects and anxiety disorders caused by acute infantile maternal deprivation in adult rats. We used 18-day-old Wistar albino rats of both sexes. The experimental groups included control (C), maternally deprived (MD), maternally deprived and treated with 0.02 μg/kg oxytocin (MD-0.02 µg/kg oxy), maternally deprived and treated with 2 μg/kg oxytocin (MD-2 µg/kg oxy). When...
Source: Biotechnic and Histochemistry - May 20, 2019 Category: Research Authors: Dayi A, Kiray M, Sisman AR, Ozbal S, Baykara B, Aksu I, Uysal N Tags: Biotech Histochem Source Type: research

Quirks of dye nomenclature. 12. Victoria's rainbow.
Abstract The identities of 18 dyes whose names begin with "Victoria" are described using their chemical structures, names and numerical identifiers. All are synthetic dyes originally synthesized in Germany during the late 19th century as colorants for textiles. Brief manufacturing details are included. All the colors of the rainbow are represented except indigo. Unusual properties including explosive tendency or toxicity are noted. Some of the applications as stains and for food coloring, anti-obesity medication and pigments for ball pen inks also are discussed. PMID: 31088175 [PubMed - as supplied ...
Source: Biotechnic and Histochemistry - May 15, 2019 Category: Research Authors: Cooksey CJ Tags: Biotech Histochem Source Type: research

Effect of melatonin on torsion and reperfusion induced pathogenesis of rat uterus.
Abstract We investigated the use of melatonin to improve fertility and reduce uterine damage caused by torsion of the uterus in pregnant rats. We used 35 pregnant rats at gestational age 18 days. The animals were randomized into five groups. Group 1 was anesthetized only. Group 2 was subjected to experimental uterine torsion of 360° and the torsion was corrected after 6 h. Group 3 was subjected to uterine torsion of 360°, the torsion was corrected after 6 h and melatonin was administered at the time of correction. Group 4 rats were subjected to 360º uterine torsion and melatonin was adm...
Source: Biotechnic and Histochemistry - May 9, 2019 Category: Research Authors: Saat N, Risvanli A, Dogan H, Onalan E, Akpolat N, Seker I, Sahna E Tags: Biotech Histochem Source Type: research

Morphological and immunohistochemical study of the midgut and fat body of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: noctuidae) treated with essential oils of the genus Piper.
avarro DMAF Abstract Essential oils are a promising alternative to insecticides. We investigated the LD50 of oils extracted from Piper corcovadensis, P. marginatum, and P. arboreum after 48 h topical contact with Spodoptera frugiperda larvae using morphometry, histochemistry and immunohistochemistry of the midgut and fat body. Chromatography revealed that E-caryophyllene was the principal compound common to the Piper species. The essential oils of P. corcovadensis, P. marginatum and P. arboreum caused deleterious changes in the midgut of S. frugiperda larvae. P. corcovadensis oil produced the lowest LD50 and ...
Source: Biotechnic and Histochemistry - May 8, 2019 Category: Research Authors: Dutra KA, Wanderley Teixeira V, Cruz GS, Silva CTS, D Assunção CG, Ferreira CGM, Monteiro ALB, Agra Neto AC, Lapa Neto CJC, Teixeira AAC, Navarro DMAF Tags: Biotech Histochem Source Type: research

Visualization of viral DNA assembly using nonfluorescent staining.
We present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilitates their use in everyday experiments. Methyl green is most promising for routine detection of viral DNA assemblies in the cytoplasm; the procedure enables ready detection of viral DNA accumulation in the cytoplasm. PMID: 31066292 [PubMed - as supplied by publisher] (Source: Biotechnic and Histochemistry)
Source: Biotechnic and Histochemistry - May 8, 2019 Category: Research Authors: Karalova EM, Hakobyan LH, Tatoyan MR, Sahakyan KT, Karalyan ZA Tags: Biotech Histochem Source Type: research

A novel thin section preparation and staining protocol to increase contrast and resolution of cell details for light microscopy.
Abstract We developed a novel sectioning and staining method to make high contrast, high resolution sections of plant tissue for light microscopy. Specimens of teosinte (Zea mays L., ssp. mexicana) root tips were fixed and embedded in Technovit 7100™ plastic resin. Thin sections, 1-2.5 μm, were cut and mounted on glass slides. The sections were either treated with RNase or not, then stained with 0.1% toluidine blue O and observed through ∞/0 objective lenses. For light microscopy, the enzyme staining procedure increased resolution and contrast. High magnification ∞/0 objective lenses produced ...
Source: Biotechnic and Histochemistry - May 2, 2019 Category: Research Authors: Niki T, Saito S, Gladish DK Tags: Biotech Histochem Source Type: research

Increased endogenous indole-3-acetic acid:abscisic acid ratio is a reliable marker of Pinus massoniana rejuvenation.
Abstract Pinus massoniana is a recalcitrant tree species for rooting in vitro. We rejuvenated 26-year-old P. massoniana trees by successive grafting. Rooting rates of rejuvenated shoots were> 83.1% after rooting induction. We compared endogenous levels of indole-3-acetic acid (IAA), abscisic acid (ABA), gibberellins (GAs) and zeatin-riboside (ZR), and the rhizogenesis ability of axillary shoots of mature and rejuvenated materials in vitro, i.e., somaplants and grafts. Enhancement of the rooting ability of mature materials in vitro following somatic embryogenesis or repeated grafting onto juvenile rootstocks was...
Source: Biotechnic and Histochemistry - May 2, 2019 Category: Research Authors: Wang Y, Yao RL Tags: Biotech Histochem Source Type: research

Altered morphology and biochemistry of the female rat liver following 900 megahertz electromagnetic field exposure during mid to late adolescence.
E Abstract Despite their benefits, technological devices such as cell phones may also have deleterious effects on human health. Considerable debate continues concerning the effects of the electromagnetic field (EMF) emitted during cell phone use on human health. We investigated the effects of exposure to 900 megahertz (MHz) EMF during mid to late adolescence on the rat liver. Control (ContGr), sham (ShmGr) and EMF (EMFGr) groups of female rats were established. We exposed the EMFGr rats daily to 900 MHz EMF on postnatal days 35-59. ShmGr rats underwent sham procedures. No procedure was performed on ContGr r...
Source: Biotechnic and Histochemistry - April 24, 2019 Category: Research Authors: Okatan DÖ, Kulaber A, Kerimoglu G, Odacı E Tags: Biotech Histochem Source Type: research

Correction.
Authors: PMID: 31017003 [PubMed - as supplied by publisher] (Source: Biotechnic and Histochemistry)
Source: Biotechnic and Histochemistry - April 24, 2019 Category: Research Tags: Biotech Histochem Source Type: research

Effects of caffeic acid phenethyl ester (CAPE) on angiogenesis, apoptosis and oxidat ıve stress ın various cancer cell lines.
Effects of caffeic acid phenethyl ester (CAPE) on angiogenesis, apoptosis and oxidatıve stress ın various cancer cell lines. Biotech Histochem. 2019 Apr 17;:1-7 Authors: Fırat F, Özgül M, Türköz Uluer E, Inan S Abstract Cancer is a common cause of death worldwide. Approximately 80% of cancer patients use complementary or alternative medicines for treatment. Caffeic acid phenethyl ester (CAPE), the main active component of propolis, exhibits cytotoxic, antiproliferative and anti-cancer effects. Despite its anticancer effects CAPE exhibits no known harmful effects toward normal ce...
Source: Biotechnic and Histochemistry - April 17, 2019 Category: Research Authors: Fırat F, Özgül M, Türköz Uluer E, Inan S Tags: Biotech Histochem Source Type: research

Protective effects of crocin on biochemistry and histopathology of experimental periodontitis in rats.
We examined histopathology and inflammatory cell infiltration in gingiva and periodontal ligament. MDA and TOS levels, and SOD and CAT activities increased significantly in rats with induced periodontitis compared to the control group, while GSH and TAS levels were decreased significantly compared to the control group. Histopathologic examination revealed inflammatory cell infiltration in gingiva epithelium and subepithelial connective tissue in the EP group. Histological damage was reduced significantly after crocin treatment compared to the EP group. Crocin supplementation may help reduce oxidative damage to periodontal ...
Source: Biotechnic and Histochemistry - April 15, 2019 Category: Research Authors: Kocaman G, Altinoz E, Erdemli ME, Gul M, Erdemli Z, Gul S, Bag HG Tags: Biotech Histochem Source Type: research

Effect of a honey and arginine-glutamine-hydroxymethylbutyrate mixture on the healing of colon anastomosis in rats immunosuppressed with tacrolimus.
Abstract We compared the effect of honey and a mixture of arginine-glutamine-hydroxymethylbutyrate (AGHMB) on healing of a descending colon anastomosis in rats that were immunosuppressed with tacrolimus (Tac). Sprague-Dawley rats were divided into four groups: untreated control, Tac, Tac + honey and Tac + AGHMB. Colon resection and anastomosis were performed on day 14 and re-laparotomy was performed on the day 21 of the study. Anastomotic bursting pressure, macroscopic adhesion score, weekly body weight changes, histopathological features and immunohistochemical staining of TGF-β1 were determined for all grou...
Source: Biotechnic and Histochemistry - April 15, 2019 Category: Research Authors: Akbulut S, Dogan Z, Baskiran A, Elbe H, Turkoz Y Tags: Biotech Histochem Source Type: research

Comparison of histochemical staining techniques for detecting mast cells in oral lesions.
Abstract Mast cells are large cells with granular cytoplasm that participate in wound healing, angiogenesis and defense against pathogens. They also contribute to inflammation by initiating innate and acquired immunity. The granules of these cells exhibit characteristic staining properties. We investigated toluidine blue, astra blue, Alcian blue-pyronin Y and May-Grunwald Giemsa stains for mast cells in various oral lesions and assessed the efficacy of each for identifying mast cells. Sections were obtained from 10 each of diagnosed cases of inflammatory fibrous hyperplasia, periapical cyst, mild dysplasia, oral s...
Source: Biotechnic and Histochemistry - April 15, 2019 Category: Research Authors: Mutsaddi S, Kotrashetti VS, Nayak RS, Pattanshetty SM Tags: Biotech Histochem Source Type: research

Effects of Oenothera biennis L. and Hypericum perforatum L. extracts on some central nervous system myelin proteins, brain histopathology and oxidative stress in mice with experimental autoimmune encephalomyelitis.
Abstract We investigated the effects of Oenothera biennis L. and Hypericum perforatum L. extracts on brain tissue histopathology, myelin oligodendrocyte glycoprotein (MOG), myelin basic protein (MBP), total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) in mice with experimental autoimmune encephalomyelitis (EAE). Forty-seven C57BL/6J mice were divided into the following groups: multiple sclerosis (MS), control (healthy mice), MS + H. perforatum treated (MS + HP), MS + O. biennis treated (MS + OB). All groups except the control group we...
Source: Biotechnic and Histochemistry - April 8, 2019 Category: Research Authors: Selek S, Esrefoglu M, Meral I, Bulut H, Caglar HG, Sonuc G, Yildiz C, Teloglu ES, Dogan N, Yuce B, Tiftik E, Bayindir N Tags: Biotech Histochem Source Type: research

Cardioprotective effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in rats.
, Fidanol Erboğa Z Abstract We investigated the effects of caffeic acid phenethyl ester (CAPE) on cardiac damage after blunt chest injury. Forty male adult Wistar albino rats were divided into four groups; control, cardiac contusion, cardiac contusion + CAPE, and CAPE. CAPE, 10 mmol/kg, was administered intraperitoneally for 7 days following cardiac contusion. Heart tissue and blood were obtained at the end of the experimental period. Cardiac histopathology was determined using hematoxylin & eosin (H & E) staining. Expression of tumor necrosis factor-alpha (TNF-α) in cardiomyocytes was det...
Source: Biotechnic and Histochemistry - April 1, 2019 Category: Research Authors: Bıçakçı N, Karaboğa I, Dökmeci AH, Güzel S, Fidanol Erboğa Z Tags: Biotech Histochem Source Type: research

TRPV5 and TRPV6 are expressed in placenta and bone tissues during pregnancy in mice.
Abstract We investigated the dynamic expression of calcium transporters, TRPV5 and TRPV6, in placenta and bone to determine their role in maternal and fetal calcium balance during gestation. In placenta, TRPV5 was expressed predominantly in syncytiotrophoblasts of the labyrinthine zone, whereas TRPV6 was expressed in spongiotrophoblasts of the junction zone. In bone, the two transporters were found in osteoblasts, osteoclasts, cartilage and bone matrices. During the first half of gestation, TRPV5 and TRPV6 levels in bone were increased on pregnancy day (P) 0.5, then decreased on P3.5 followed by a slight increase ...
Source: Biotechnic and Histochemistry - March 27, 2019 Category: Research Authors: Li SH, Yin HB, Ren MR, Wu MJ, Huang XL, Li JJ, Luan YP, Wu YL Tags: Biotech Histochem Source Type: research

The role of HMGB1 in liver inflammation in obese rats.
Abstract Obesity is a chronic disease that is characterized by increased body fat owing to imbalance between consumed and expended energy. Inflammation generally is accompanied by accumulation of excess lipid in adipose tissue and liver. High mobility group box-1 (HMGB1) participates in the pathogenesis of inflammatory diseases. We investigated the relation of the number of HMGB1 positive cells to body mass index (BMI), liver inflammation and the number of Kupffer cells. We divided 18 female Wistar albino rats into two groups: group 1, untreated control fed normal commercial rat diet and group 2, obese rats fed a ...
Source: Biotechnic and Histochemistry - March 27, 2019 Category: Research Authors: Erdal M, Altunkaynak BZ, Kocaman A, Alkan I, Ozata M Tags: Biotech Histochem Source Type: research

Immunostaining characteristics of irisin in benign and malignant renal cancers.
n S Abstract We investigated the expression of irisin in renal cancers using immunocytochemistry. Irisin has been reported to exhibit anticancer properties. The study groups consisted of 22 cases each of control renal tissue, oncocytoma, chromophobe renal cell carcinoma (RCC), clear cell RCC (Fuhrman nuclear grades 1, 2, 3 and 4) and papillary RCC. We evaluated 10 slides for each of 176 cases. Slides were immunostained for irisin and histoscores were calculated for the prevalence and strength of immunostaining. Fuhrman nuclear grade 1, 2, 3 clear cell RCC and papillary RCC exhibited no irisin immunoreactivity. Iri...
Source: Biotechnic and Histochemistry - March 21, 2019 Category: Research Authors: Kuloğlu T, Artaş G, Yardim M, Sahin I, Aydin Y, Beyoğlu N, Özercan IH, Yalcin MH, Ugur K, Aydin S Tags: Biotech Histochem Source Type: research

A rapid and simple method for visualizing milky spots in large fixed tissue samples of the human greater omentum.
Abstract Milky spots are unique lymphoid structures in the greater omentum that participate in both immune homeostasis of the peritoneal cavity and formation of omental metastases. We developed a rapid and simple staining method to enable macro- or stereomicroscopic identification of these miniscule structures in large samples of fixed human greater omentum. By immersing approximately 6 × 4 cm samples of omental tissue in hematoxylin, these samples could be evaluated quickly for the presence of milky spots. We used an alum hematoxylin variant containing 1 g hematoxylin, 50 g aluminium amm...
Source: Biotechnic and Histochemistry - March 21, 2019 Category: Research Authors: Schurink B, Cleypool CGJ, Bleys RLAW Tags: Biotech Histochem Source Type: research