Socio-environmental determinants of the delay in the first dental visit: results of two population-based cohort studies in Brazil.
The objective of this study was to describe the timing of the first dental visit and investigate the association of socioeconomic and behavioral factors with dental visit delay among 10/11-year-old children from two live-birth population cohorts with extremely contrasting socioeconomic profiles. Follow-up data (2004-2005) from cohorts of Ribeirão Preto (RP) (n=790) and São Luís (SL) (n=673) were evaluated. Delay in dental visit was defined as not visiting a dentist before the age of 7. Covariates included family socioeconomic characteristics, mother-related health behavior, and child-related characteri...
Source: Braz J Med Biol Res - December 3, 2020 Category: Research Authors: Soares ALFH, Ribeiro CCC, Thomaz EBAF, Queiroz RCS, Alves CMC, Ferraro AA, Silva AAM, Bettiol H, Barbieri MA, Saraiva MCP Tags: Braz J Med Biol Res Source Type: research

Impact of chronic obstructive pulmonary disease on linear and nonlinear dynamics of heart rate variability in patients with heart failure.
The objective of this study was to investigate the impact of chronic obstructive pulmonary disease (COPD)-heart failure (HF) coexistence on linear and nonlinear dynamics of heart rate variability (HRV). Forty-one patients (14 with COPD-HF and 27 HF) were enrolled and underwent pulmonary function and echocardiography evaluation to confirm the clinical diagnosis. Heart rate (HR) and R-R intervals (iRR) were collected during active postural maneuver (APM) [supine (10 min) to orthostasis (10 min)], respiratory sinus arrhythmia maneuver (RSA-M) (4 min), and analysis of frequency domain, time domain, and nonlinear HRV. We found ...
Source: Braz J Med Biol Res - December 3, 2020 Category: Research Authors: Goulart CL, Caruso FR, Arêas GPT, Santos PBD, Camargo PF, Carvalho LCS, Roscani MG, Mendes RG, Borghi-Silva A Tags: Braz J Med Biol Res Source Type: research

Examination of Ureaplasma urealyticum and Mycoplasma hominis in 4082 Chinese patients.
The objective of this study was to analyze the infection rate and drug resistance of Ureaplasma urealyticum (UU) and Mycoplasma hominis (MH) in the genitourinary tract of Chinese patients. From December 2018 to June 2019, vaginal secretion or urinary secretion of outpatients in our hospital were selected for culture and drug sensitivity analysis of Ureaplasma urealyticum and Mycoplasma hominis. In 4082 Chinese samples, 1567 Mycoplasma were detected, a detection rate of 38.39%, among which 1366 cases were UU single positive, accounting for 33.47%, 15 cases were MH single positive, accounting for 0.36%, 186 cases were UU and...
Source: Braz J Med Biol Res - December 3, 2020 Category: Research Authors: Zheng WW, Zhang WJ, Cui D, Nie ZC, Ding BS, Cheng JH, Mei CZ Tags: Braz J Med Biol Res Source Type: research

Clinical significance of sirtuin 1 level in sepsis: correlation with disease risk, severity, and mortality risk.
In conclusion, measurement of serum SIRT1 might assist with the optimization of disease assessment, management strategies, and survival surveillance in sepsis patients. PMID: 33263643 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - December 3, 2020 Category: Research Authors: Cheng X, Zhang S, Wen Y, Shi Z Tags: Braz J Med Biol Res Source Type: research

Efficacy of oseltamivir compared with zanamivir in COPD patients with seasonal influenza virus infection: a randomized controlled trial.
Abstract Influenza viruses exacerbate chronic obstructive pulmonary disease (COPD) with considerable morbidity and mortality. Zanamivir and oseltamivir are effective in treating influenza. However, their efficacy in relieving influenza symptoms in COPD patients remains unknown, with the lack of controlled trials in this subject. Therefore, we conducted this randomized controlled trial to investigate the clinical efficacy of both interventions in this population. Patients were allocated to two groups (80 patients each): oseltamivir (OSELTA) and zanamivir (ZANA) groups. Oseltamivir (75 mg) was orally administered tw...
Source: Braz J Med Biol Res - December 3, 2020 Category: Research Authors: Li M, Han GC, Chen Y, Du WX, Liu F, Chi YM, Du JF Tags: Braz J Med Biol Res Source Type: research

A duplex probe-directed recombinase amplification assay for detection of single nucleotide polymorphisms on 8q24 associated with prostate cancer.
In conclusion, the duplex-PDRA assay is an effective method for the simultaneous detection of two SNPs and shows great potential for widespread use in research and clinical settings. PMID: 33263645 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - December 3, 2020 Category: Research Authors: Duan Q, Li X, He X, Shen X, Cao Y, Zhang R, Bai X, Zhang J, Ma X Tags: Braz J Med Biol Res Source Type: research

Determinants of the introduction of early complementary feeding before and after the third month of life: a multinomial analysis.
The objective of this study was to analyze factors associated with the introduction of ECF in two different moments of the infant's life: from zero to three and from four to five months of age. A cohort with 3,306 dyads studied in the BRISA survey in São Luis/MA in 2010 was used. Questionnaires were applied at birth and at follow-up when the infants were 15 to 36 months of age of women with more than 20 weeks of gestational age, residing in this municipality. A multivariate model of multinomial logistic regression was used to verify associations between independent variables and ECF at 0 to 3 months and at 4 to 5 mo...
Source: Braz J Med Biol Res - November 26, 2020 Category: Research Authors: Trovão T, Cavalcante MCV, Rodrigues MC, Ferraro AA, Bettiol H, Saraiva MCP, Lamy ZC, Lamy-Filho F Tags: Braz J Med Biol Res Source Type: research

Uterine vascular resistance and other maternal factors associated with the risk of developing hypertension during pregnancy.
The objective of the present study was to determine the increase in relative risk for developing hypertensive disorders of pregnancy based on the evaluation of pregnant women between 20 and 25 weeks of gestation, and to correlate the findings at this period with the outcome of pregnancy. We conducted a prospective cohort study, with a convenience sample of 1417 patients evaluated at this gestational age, of which 1306 were contacted at childbirth. We detected an increased relative risk of 2.69 (95%CI: 1.86 to 3.89) associated with pulsatility index of the uterine arteries, a 2.8 increase (95%CI: 1.58 to 5.03) in relative r...
Source: Braz J Med Biol Res - November 26, 2020 Category: Research Authors: Martins LAB, Veiga ECA, Ribeiro CCC, Simões VMF, Cardoso VC, Bettiol H, Barbieri MA, Cavalli RC Tags: Braz J Med Biol Res Source Type: research

Mogens Lesner Glass (1946-2018).
PMID: 33237132 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - November 9, 2020 Category: Research Authors: Wang T, Wood S Tags: Braz J Med Biol Res Source Type: research

Visible DNA microarray and loop-mediated isothermal amplification (LAMP) for the identification of Cryptococcus species recovered from culture medium and cerebrospinal fluid of patients with meningitis.
The objective of this study was to design two molecular assays, visible microarray platforms and loop-mediated isothermal amplification (LAMP), to identify Cryptococcus spp. and the species neoformans and gattii from the cerebral spinal fluid (CSF). To identify Cryptococcus and the two species, we designed two microarrays DNA platforms based on the internal transcribed spacer (ITS) region and CAP59 gene and LAMP assays specific for Cryptococcus species. The assays were tested using CSF from patients with cryptococcal meningitis. CSF from patients with cryptococcal meningitis was cultured in Sabouraud culture medium, and th...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Stivanelli P, Tararam CA, Trabasso P, Levy LO, Melhem MSC, Schreiber AZ, Moretti ML Tags: Braz J Med Biol Res Source Type: research

A cutoff thyroglobulin value suggestive of distant metastases in differentiated thyroid cancer patients.
Abstract Serum thyroglobulin is used as part of the early postoperative assessment of differentiated thyroid cancer (DTC) since there is a clear relationship between an increased risk of recurrence and persistent disease after initial treatment and high postoperative stimulated thyroglobulin (ps-Tg) values. Thus, although ps-Tg above 10-30 ng/mL is considered an independent predictor of worse prognosis, the value that is associated with distant metastases is not defined. Thus, this was our objective. We selected 655 DTC patients from a nuclear medicine department database (Irmandade Santa Casa de Misericórd...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Couto JS, Almeida MFO, Trindade VCG, Marone MMS, Scalissi NM, Cury AN, Ferraz C, Padovani RP Tags: Braz J Med Biol Res Source Type: research

Comparative overview of the effects of aerobic and resistance exercise on anxiety-like behavior, cognitive flexibility, and hippocampal synaptic plasticity parameters in healthy rats.
This study aimed to compare the effects of an aerobic exercise protocol on a treadmill and a resistance exercise protocol on a ladder apparatus on anxiety-like behavior, cognitive flexibility, and neuroplasticity parameters in healthy animals. Adult male Wistar rats were divided into three groups: sedentary control, aerobic training, and resistance training. Subsequently, they were evaluated in the elevated plus-maze (EPM), light-dark box, and modified hole board (mHB) tests. The expressions of synaptophysin and postsynaptic plasticity protein 95 in the dorsal and ventral hippocampus were analyzed by immunofluorescence. Th...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Segabinazi E, Gasperini NF, Faustino AM, Centeno R, Santos ASD, Almeida W, Bronauth LP, Marcuzzo S, Pereira LO Tags: Braz J Med Biol Res Source Type: research

Increased elastic modulus of the synovial membrane in a rat ACLT model of osteoarthritis revealed by atomic force microscopy.
This study aimed to explore changes in nanoscale elastic modulus of the synovium using atomic force microscopy (AFM) in addition to investigate changes in synovial histomorphology and secretory function in osteoarthritis (OA) in a rat anterior cruciate ligament transection (ACLT) model. Sprague-Dawley rats were randomly assigned to sham control and ACLT OA groups. All right knee joints were harvested at 4, 8, or 12 weeks (W) after surgery for histological assessment of cartilage damage and synovitis in both the anterior and posterior capsules. AFM imaging and nanoscale biomechanical testing were conducted to measure the el...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Dai S, Liang T, Fujii T, He S, Zhang F, Jiang H, Liu B, Shi X, Luo Z, Yang H Tags: Braz J Med Biol Res Source Type: research

Antiproliferative effect of mifepristone (RU486) on human neuroblastoma cells (SK-N-SH): in vitro and in vivo studies.
Abstract RU486 (mifepristone), a glucocorticoid and progesterone receptor antagonist, has been reported to exert antiproliferative effects on tumor cells. Experiments were performed to analyze the effects of RU486 on the proliferation of the human neuroblastoma, both in vitro and in vivo, using the human neuroblastoma SK-N-SH cell line. The exposure in vitro of SK-N-SH cells to RU486 revealed a dose-dependent inhibition of 3H-thymidine incorporation due to a rapid but persistent inhibition of MAPKinase activity and ERK phosphorylation. A significant decrease of SK-N-SH cell number was evident after 3, 6, and 9 day...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Casulari LA, Dondi D, Pratesi G, Piva F, Milani M, Piccolella M, Maggi R Tags: Braz J Med Biol Res Source Type: research

P-glycoprotein associated with diabetes mellitus and survival of patients with pancreatic cancer: 8-year follow-up.
This study aimed to explore the potential prognostic value of P-gp and CYP3A4 in PaC patients in the context of DM through long-term follow-up. We retrospectively reviewed the medical records of patients with PaC admitted at The First People's Hospital of Changzhou, Jiangsu, China, from January 2011 to November 2019 and identified two cohorts of adult patients with PaC, including 24 with DM and 24 without DM (non-DM). The baseline clinical characteristics and outcomes were compared. Immunohistochemistry showed that protein expression of P-gp, but not CYP3A, in duodenum tissues was significantly upregulated in PaC patients ...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Hu N, Wang H, Qian Q, Jiang Y, Xie J, Zhang D, Li Q, Zou S, Chen R Tags: Braz J Med Biol Res Source Type: research

Association between socioeconomic markers and adult telomere length differs according to sex: Pro-Sa úde study.
Association between socioeconomic markers and adult telomere length differs according to sex: Pro-Saúde study. Braz J Med Biol Res. 2020;53(11):e10223 Authors: Normando P, Bezerra FF, Santana BA, Calado RT, Santos-Rebouças CB, Epel ES, Faerstein E Abstract Understanding the social determinants of telomere length is critical to evaluate the risk of early biological aging. We investigated sex differences on the association between socioeconomic status (SES) and demographic markers and leukocyte telomere length (LTL) in Brazilian adults. This cross-sectional study was conducted in a subsamp...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Normando P, Bezerra FF, Santana BA, Calado RT, Santos-Rebouças CB, Epel ES, Faerstein E Tags: Braz J Med Biol Res Source Type: research

Effects of the prophylactic use of escitalopram on the prognosis and the plasma copeptin level in patients with acute cerebral infarction.
This study aimed to investigate whether the routine administration of escitalopram for three months would improve the prognosis of patients with ischemic stroke and decrease the plasma copeptin level. A total of 97 patients with acute cerebral infarction were randomly allocated to receive escitalopram (5-10 mg once per day, orally; n=49) or not to receive escitalopram (control group; n=48) for 12 weeks starting at 2-7 days after the onset of stroke. Both groups received conventional treatments, including physiotherapy and secondary prevention of stroke. The National Institutes of Health Stroke Scale (NIHSS) score was used ...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Cao JX, Liu L, Sun YT, Zeng QH, Wang Y, Chen JC Tags: Braz J Med Biol Res Source Type: research

lncRNA H19 acts as a ceRNA to regulate the expression of CTGF by targeting miR-19b in polycystic ovary syndrome.
In conclusion, our study indicated that lncRNA H19 acted as a ceRNA to bind to miR-19b via a "sponge" to regulate the effect of CTGF on KGN cells, which may play a vital role in PCOS. PMID: 33053114 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Sun X, Yan X, Liu K, Wu M, Li Z, Wang Y, Zhong X, Qin L, Huang C, Wei X Tags: Braz J Med Biol Res Source Type: research

Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway.
The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared ...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Yao YE, Zhang JH, Chen XJ, Huang JL, Sun QX, Liu WW, Zeng H, Li CQ Tags: Braz J Med Biol Res Source Type: research

A novel stem cell therapy for hepatitis B virus-related acute-on-chronic liver failure.
Abstract The aim of this study was to propose a stem cell therapy for hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) based on plasma exchange (PE) for peripheral blood stem cell (PBSC) collection and examine its safety and efficacy. Sixty patients (n=20 in each group) were randomized to PE (PE alone), granulocyte colony-stimulating factor (G-CSF) (PE after G-CSF treatment), and PBSC transplantation (PBSCT) (G-CSF, PE, PBSC collection and hepatic artery injection) groups. Patients were followed-up for 24 weeks. Liver function and adverse events were recorded. Survival analysis was performed. ...
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Zhu B, You S, Rong Y, Yu Q, Lv S, Song F, Liu H, Wang H, Zhao J, Li D, Liu W, Xin S Tags: Braz J Med Biol Res Source Type: research

lncRNA TUG1 promotes proliferation and differentiation of osteoblasts by regulating the miR-545-3p/CNR2 axis.
In conclusion, TUG1 accelerated the proliferation and differentiation of osteoblasts by sponging miR-545-3p and increasing CNR2 expression, which might provide a new biomarker for bone diseases. PMID: 33053117 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - October 20, 2020 Category: Research Authors: Hao R, Wang B, Wang H, Huo Y, Lu Y Tags: Braz J Med Biol Res Source Type: research

AQP5 regulates the proliferation and differentiation of epidermal stem cells in skin aging.
This study aimed to investigate epidermal stem cells during the process of aging. This study enrolled 9 volunteers with benign pigmented nevus for clinical dermatologic surgery. The phenotypes associated with skin aging changes such as skin wrinkles and elasticity of the unexposed/healthy parts near benign pigmented skin were measured, and epidermal stem cells from this region were isolated for transcriptome sequencing. The results showed that epidermal stem cells could be obtained by magnetic activated cell sorting (MACS) with high purity. Results of the transcriptome sequencing revealed that aquaporin (AQP)5 significantl...
Source: Braz J Med Biol Res - September 24, 2020 Category: Research Authors: Zhou J, Dong Y, Liu J, Ren J, Wu J, Zhu N Tags: Braz J Med Biol Res Source Type: research

Amifostine protects from the peripheral sensory neuropathy induced by oxaliplatin in mice.
This study investigated the antinociceptive effect of amifostine and its potential neuroprotective mechanisms on the oxaliplatin-related peripheral sensory neuropathy in mice. Oxaliplatin (1 mg/kg) was injected intravenously in Swiss albino male mice twice a week (total of nine injections), while amifostine (1, 5, 25, 50, and 100 mg/kg) was administered subcutaneously 30 min before oxaliplatin. Mechanical and thermal nociceptive tests were performed once a week for 49 days. Additionally, c-Fos, nitrotyrosine, and activating transcription factor 3 (ATF3) immunoexpressions were assessed in the dorsal root ganglia. In all dos...
Source: Braz J Med Biol Res - September 24, 2020 Category: Research Authors: Pereira AF, Lino JA, Alves BWF, Lisboa MRP, Pontes RB, Leite CAVG, Nogueira RB, Lima-Júnior RCP, Vale ML Tags: Braz J Med Biol Res Source Type: research

Absence of norovirus contamination in shellfish harvested and commercialized in the Northeast coast of Brazil.
Abstract Norovirus (NoV) is the main cause of gastroenteritis outbreaks worldwide. Although NoV spreads mainly from person to person, it is estimated that a large proportion of NoV outbreaks are caused by foodborne transmission. Bivalve mollusks are one of the most important foods involved in NoV transmission to humans. Little is known about NoV prevalence in shellfish harvested and commercialized in Brazil. The aim of this study was to map, for the first time, the distribution of NoV contamination in oysters and mussels harvested and commercialized in the coast of Pernambuco state, northeast Brazil. A total of 38...
Source: Braz J Med Biol Res - September 24, 2020 Category: Research Authors: Guarines KM, Mendes RPG, Cordeiro MT, Miagostovich MP, Gil LHVG, Green KY, Pena LJ Tags: Braz J Med Biol Res Source Type: research

Endothelial progenitor cells in the peripheral blood of patients with moyamoya disease labeled with superparamagnetic iron oxide in vitro for MRI detection.
Abstract Moyamoya disease (MMD) is currently thought to involve endothelial progenitor cells (EPCs). We investigated whether superparamagnetic iron oxide (SPIO) can be used to label EPCs. Mononuclear cells from 10 moyamoya disease patients were isolated, and cluster of differentiation 133 (CD133) positive cells sorted by magnetic-activated cell sorting were cultured in vitro. The positive rates of CD133, vascular endothelial growth factor receptor (VEGFR)-2, and cluster of differentiation 34 (CD34) were detected by flow cytometry. The cells were co-cultured with fluorescence labeled Dil-acetylated-low-density lipo...
Source: Braz J Med Biol Res - September 24, 2020 Category: Research Authors: Wang X, Zhang W, Yin G Tags: Braz J Med Biol Res Source Type: research

Retraction notice for: "MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts" [Braz J Med Biol Res (2018) 51(12): e7574].
Retraction notice for: "MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts" [Braz J Med Biol Res (2018) 51(12): e7574]. Braz J Med Biol Res. 2020;53(10):e7574retraction Authors: PMID: 32813849 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Circulating nucleic acids in the plasma and serum as potential biomarkers in neurological disorders.
Abstract Neurological diseases are responsible for approximately 6.8 million deaths every year. They affect up to 1 billion people worldwide and cause significant disability and reduced quality of life. In most neurological disorders, the diagnosis can be challenging; it frequently requires long-term investigation. Thus, the discovery of better diagnostic methods to help in the accurate and fast diagnosis of neurological disorders is crucial. Circulating nucleic acids (CNAs) are defined as any type of DNA or RNA that is present in body biofluids. They can be found within extracellular vesicles or as cell-free DNA ...
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Authors: Bruno DCF, Donatti A, Martin M, Almeida VS, Geraldis JC, Oliveira FS, Dogini DB, Lopes-Cendes I Tags: Braz J Med Biol Res Source Type: research

Neck and waist circumference values according to sex, age, and body-mass index: Brazilian Longitudinal Study of Adult Health (ELSA-Brasil).
We present estimated 25th, 50th, 75th, and 90th percentiles for NC and WC values, according to BMI, age, and sex. Predicted interquartile intervals for NC values varied from 1.6 to 3.8 cm and, for WC values, from 5.1 to 10.3 cm. Median NC was not associated with age in men (P=0.11) nor in women (P=0.79). However, median WC increased with advancing age in both sexes (P
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Authors: Moura BAB, Santos IS, Goulart AC, Schmidt MI, Lotufo PA, Bensenor IM, Baena CP Tags: Braz J Med Biol Res Source Type: research

Enriched environment improves sevoflurane-induced cognitive impairment during late-pregnancy via hippocampal histone acetylation.
Abstract Fetal exposure to sevoflurane induces long-term cognitive impairment. Histone acetylation regulates the transcription of genes involved in memory formation. We investigated whether sevoflurane exposure during late-pregnancy induces neurocognitive impairment in offspring, and if this is related to histone acetylation dysfunction. We determined whether the effects could be reversed by an enriched environment (EE). Pregnant rats were exposed to 2.5% sevoflurane or control for 1, 3, or 6 h on gestational day 18 (G18). Sevoflurane reduced brain-derived neurotrophic factor (BDNF), acetyl histone H3 (Ac-H3), and...
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Authors: Yu Z, Wang J, Zhang P, Wang J, Cui J, Wang H Tags: Braz J Med Biol Res Source Type: research

Does the addition of vitamin E to conventional UHMWPE improve the wear performance of hip acetabular cups? Micro-Raman characterization of differently processed polyethylene acetabular cups worn on a hip joint simulator.
Abstract In knee replacements, vitamin E-doped ultra-high molecular weight polyethylene (UHMWPE) shows a better wear behavior than standard UHMWPE. Therefore, different sets of polyethylene (PE) acetabular cups, i.e. standard UHMWPE and cross-linked polyethylene irradiated with 50 kGy and 75 kGy, were compared, at a molecular level, with vitamin E-doped UHMWPE to evaluate their wear performance after being tested on a hip joint simulator for five million cycles. Unworn control and worn acetabular cups were analyzed by micro-Raman spectroscopy to gain insight into the effects of wear on the microstructure and phase...
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Authors: Di Foggia M, Affatato S, Taddei P Tags: Braz J Med Biol Res Source Type: research

Retraction notice for: "PDK2 promotes chondrogenic differentiation of mesenchymal stem cells by upregulation of Sox6 and activation of JNK/MAPK/ERK pathway" [Braz J Med Biol Res (2017) 50(2): e5988].
Retraction notice for: "PDK2 promotes chondrogenic differentiation of mesenchymal stem cells by upregulation of Sox6 and activation of JNK/MAPK/ERK pathway" [Braz J Med Biol Res (2017) 50(2): e5988]. Braz J Med Biol Res. 2020;53(10):e5988retraction Authors: PMID: 32813854 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Retraction notice for: "Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage" [Braz J Med Biol Res (2018) 51(2): e6611].
Retraction notice for: "Ginsenoside Rg1 protects human renal tubular epithelial cells from lipopolysaccharide-induced apoptosis and inflammation damage" [Braz J Med Biol Res (2018) 51(2): e6611]. Braz J Med Biol Res. 2020;53(10):e6611retraction Authors: PMID: 32813855 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Optical coherence tomography versus intravascular ultrasound in patients with myocardial infarction: a diagnostic performance study of pre-percutaneous coronary interventions.
The objective of the study was to compare quantitative and qualitative parameters of frequency domain optical coherence tomography (FDOCT) with those of intravascular ultrasound and coronary angiography in patients with acute myocardial infarction. Diagnostic parameters of coronary angiography, intravascular ultrasound, and FDOCT of 250 patients with coronary artery disease who required admission diagnosis were included in the analyses. Minimum lumen diameter detected by FDOCT was larger than that detected by quantitative coronary angiography (2.11±0.1 vs 1.89±0.09 mm, P
Source: Braz J Med Biol Res - August 21, 2020 Category: Research Authors: Niu Z, Lv X, Zhang J, Bao T Tags: Braz J Med Biol Res Source Type: research

Hippocampal formation volume, its subregions, and its specific contributions to visuospatial memory tasks.
Abstract Visuospatial memory (VSM) is the ability to represent and manipulate visual and spatial information. This cognitive function depends on the functioning of the hippocampal formation (HF), located in the medial portion of the temporal cortex. The present study aimed to investigate whether there is an association between the volume of the HF and performance in VSM tests. High-resolution structural images (T1) and neuropsychological tests evaluating VSM were performed on 31 healthy individuals. A VSM index was created by grouping 5 variables from 5 tasks (4 from the CANTAB battery and 1 from the Rey-Osterriet...
Source: Braz J Med Biol Res - July 30, 2020 Category: Research Authors: Shavitt T, Johnson INS, Batistuzzo MC Tags: Braz J Med Biol Res Source Type: research

Past, present, and future of COVID-19: a review.
Abstract SARS-CoV-2 has recently emerged, becoming a global threat, affecting directly all human beings owing to its morbidity and mortality and indirectly, due to the enormous economic and psychological impact produced by social isolation, the most effective measure so far, but unsustainable for a long period. The scientific effort to understand and control SARS-CoV-2 transmission and clinical impact has been huge, and important achievements are highlighted in this review. Diagnosis is central and is the first step in recognizing and fighting any infectious agent. Instrumental to that is the quality of the data, ...
Source: Braz J Med Biol Res - July 30, 2020 Category: Research Authors: Romano CM, Chebabo A, Levi JE Tags: Braz J Med Biol Res Source Type: research

Clostridium difficile toxins or infection induce upregulation of adenosine receptors and IL-6 with early pro-inflammatory and late anti-inflammatory pattern.
en CA Abstract Clostridium difficile causes intestinal inflammation, which increases adenosine. We compared the expression of adenosine receptors (AR) subtypes A1, A2A, A2B, and A3 in HCT-8, IEC-6 cells, and isolated intestinal epithelial cells, challenged or not with Clostridium difficile toxin A and B (TcdA and TcdB) or infection (CDI). In HCT-8, TcdB induced an early A2BR expression at 6 h and a late A2AR expression at 6 and 24 h. In addition, both TcdA and TcdB increased IL-6 expression at all time-points (peak at 6 h) and PSB603, an A2BR antagonist, decreased IL-6 expression and production. In isolated cecum ...
Source: Braz J Med Biol Res - July 30, 2020 Category: Research Authors: Foschetti DA, Braga-Neto MB, Bolick D, Moore J, Alves LA, Martins CS, Bomfin LE, Santos A, Leitão R, Brito G, Warren CA Tags: Braz J Med Biol Res Source Type: research

Antiproliferative and photoprotective activities of the extracts and compounds from Calea fruticosa.
o AMD Abstract In this paper, we complement our previous study on the antiproliferative activity of Calea fruticosa (Asteraceae) by isolating the compounds apigenin-4',7-dimethyl ether (1), budlein A (2), quercetin (3), and cichoriin (4) from the plant's aerial parts. The antiproliferative activity of these compounds was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines. Compound 3 displayed moderate antiproliferative activity in three cell lines (HCT-116, PC-3, and SF-295, with cell growth inhibition values of 72.97, 74.55, and 68.94%) and hi...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Seregheti TMQ, Pinto APR, Gonçalves MDC, Antunes ADS, Almeida WADS, Machado RS, Silva JN, Ferreira PMP, Pessoa C, Santos VMRD, Nascimento AMD Tags: Braz J Med Biol Res Source Type: research

Fulminant type 1 diabetes mellitus in pregnancy.
Abstract Fulminant type 1 diabetes mellitus (FT1DM) has received clinical attention for its low incidence and poor prognosis. Currently, few cases of FT1DM are associated with pregnancy in clinical practice, but it poses a great threat to the life of mothers and infants. Here, we present two cases of FT1DM in pregnancy. In Case 1, the patient was a 26-year-old woman who was admitted to the hospital with reduced fetal movement. She was diagnosed with FT1DM and delivered a dead female fetus. Lispro and lantus were administered to control blood glucose, and lipoic acid for antioxidant therapy. In Case 2, the patient ...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Li CY, Li Y, You ZY, Liu YR, Wang YJ, Mu T, Zhao CZ, Yi ZM, Zhang YW Tags: Braz J Med Biol Res Source Type: research

Identification of potential molecular mechanisms and small molecule drugs in myocardial ischemia/reperfusion injury.
Abstract Myocardial ischemia/reperfusion (MI/R) injury is a complex phenomenon that causes severe damage to the myocardium. However, the potential molecular mechanisms of MI/R injury have not been fully clarified. We identified potential molecular mechanisms and therapeutic targets in MI/R injury through analysis of Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were found between MI/R injury and normal samples, and overlapping DEGs were found between GSE61592 and GSE67308. Gene Ontology (GO) and pathway analysis were performed for overlapping DEGs by Database for Annotation, Visuali...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Jiang T, Liu Y, Chen B, Si L Tags: Braz J Med Biol Res Source Type: research

An intensive education program for caregivers ameliorates anxiety, depression, and quality of life in patients with drug-resistant temporal lobe epilepsy and mesial temporal sclerosis who underwent cortico-amygdalohippocampectomy.
In conclusion, CIEP for caregivers contributed to the recovery of anxiety and depression as well as the improvement of QOL in patients with drug-resistant TLE-MTS who underwent CAH. PMID: 32696820 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Wang Y, Hou D, Wu X, Qiu L, Chen H, Xin J, Yan Z, Sun M Tags: Braz J Med Biol Res Source Type: research

CLEC3B protects H9c2 cardiomyocytes from apoptosis caused by hypoxia via the PI3K/Akt pathway.
Abstract Ischemic heart disease (IHD) is one of the leading causes of death worldwide. C-type lectin domain family 3 member B (CLEC3B) is a C-type lectin superfamily member and is reported to promote tissue remodeling. The serum levels of CLEC3B are downregulated in patients with cardiovascular disease. However, the molecular mechanisms of CLEC3B in IHD is not well-characterized. Therefore, we overexpressed CLEC3B and silenced CLEC3B in H9c2 rat cardiomyocytes for the first time. We then constructed a model of IHD in vitro through culturing H9c2 cardiomyocytes in serum-free medium under oxygen-deficit conditions. ...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Lv F, Wang Z, Huang Y, Si A, Chen Y Tags: Braz J Med Biol Res Source Type: research

MicroRNA-770-5p contributes to podocyte injury via targeting E2F3 in diabetic nephropathy.
Abstract Diabetic nephropathy (DN) has been identified as the major cause of end-stage renal disease (ESRD) in most developed countries. MicroRNA-770-5p depletion could repress high glucose (HG)-triggered apoptosis in podocytes, and downregulation of E2F transcription factor 3 (E2F3) could facilitate podocyte injury. Nevertheless, whether E2F3 is involved in miR-770-5p knockdown-mediated improvement of DN is still unclear. The expression levels of miR-770-5p and E2F3 were detected in HG-treated podocytes by RT-qPCR. The expression levels of E2F3, apoptosis-related proteins Bcl-2 related X protein (Bax), B-cell lym...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Guo J, Han J, Liu J, Wang S Tags: Braz J Med Biol Res Source Type: research

Estrogen attenuates TGF- β1-induced EMT in intrauterine adhesion by activating Wnt/β-catenin signaling pathway.
In this study, we aimed to investigate the effects of estrogen on epithelial-mesenchymal transition (EMT) in normal and fibrotic endometrium, and the role of estrogen and Wnt/β-catenin signaling in the formation of endometrial fibrosis. CCK-8 and immunofluorescence assay were performed to access the proliferation of different concentrations of estrogen on normal human endometrial epithelial cells (hEECs). qRT-PCR and western blot assay were utilized to explore the effect of estrogen on EMT in normal and fibrotic endometrium, and main components of Wnt/β-catenin signaling pathway in vitro. Hematoxylin and eosin an...
Source: Braz J Med Biol Res - July 9, 2020 Category: Research Authors: Cao J, Liu D, Zhao S, Yuan L, Huang Y, Ma J, Yang Z, Shi B, Wang L, Wei J Tags: Braz J Med Biol Res Source Type: research

Erratum for: "Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition" [Braz J Med Biol Res (2020) 53(4): e8882].
Erratum for: "Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition" [Braz J Med Biol Res (2020) 53(4): e8882]. Braz J Med Biol Res. 2020;53(7):e8882erratum Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20198882]. PMID: 32555930 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - June 21, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Development of a three-channel automatic climbing training system for rat rehabilitation after ischemic stroke.
Abstract This paper reports the development of a three-channel automatic speed-matching climbing training system that could train three rats at the same time for rehabilitation after an ischemic stroke. An infrared (IR) remote sensor was installed at the end of each channel to monitor the real-time position of a climbing rat. This research was carried out in five stages: i) system design; ii) hardware circuit; iii) running speed control; iv) functional testing; and v) verification using an animal model of cerebral stroke. The rehabilitated group significantly outperformed the middle cerebral artery occlusion (MCAo...
Source: Braz J Med Biol Res - June 21, 2020 Category: Research Authors: Chen CC, Chang CP Tags: Braz J Med Biol Res Source Type: research

Correlation between amyloid deposits affecting renal compartments and glomerular filtration rate during renal biopsy in a renal amyloidosis case series.
Abstract Amyloidosis comprises a group of disorders that accumulate modified autologous proteins in organs, mainly the kidneys. Few studies have addressed the amyloid compartmental distribution and associated clinical outcomes. The aim of this study was to present a case series of renal amyloidosis correlating histopathological data with glomerular filtration rate (GFR) during kidney biopsy. We studied 53 cases reviewed by nephropathologists from 2000 to 2018 in a single kidney biopsy center in Brazil. GFR was estimated using the CKD-EPI formula. Cases were divided into Group A ≥60 and Group B
Source: Braz J Med Biol Res - May 20, 2020 Category: Research Authors: Fonseca EO, Caldas MLR, Soares Filho PJ, Almeida JR Tags: Braz J Med Biol Res Source Type: research

Animal models of human atherosclerosis: current progress.
Abstract Atherosclerosis retains the leading position among the causes of global morbidity and mortality worldwide, especially in the industrialized countries. Despite the continuing efforts to investigate disease pathogenesis and find the potential points of effective therapeutic intervention, our understanding of atherosclerosis mechanisms remains limited. This is partly due to the multifactorial nature of the disease pathogenesis, when several factors so different as altered lipid metabolism, increased oxidative stress, and chronic inflammation act together leading to the formation and progression of atheroscle...
Source: Braz J Med Biol Res - May 20, 2020 Category: Research Authors: Poznyak AV, Silaeva YY, Orekhov AN, Deykin AV Tags: Braz J Med Biol Res Source Type: research

Voxel-wise meta-analysis of structural changes in gray matter of Parkinson's disease patients with mild cognitive impairment.
Abstract Evidence from previous voxel-based morphometry (VBM) studies indicates that widespread brain regions are involved in Parkinson's disease with mild cognitive impairment (PD-MCI). However, the spatial localization reported for gray matter (GM) abnormalities is heterogeneous. The aim of the present study was to quantitatively integrate studies on GM abnormalities observed in PD-MCI in order to determine whether a pattern exists. Eligible whole-brain VBM studies were identified by a systematic search of articles in PubMed and EMBASE databases spanning from 1995 to January 1, 2019. A meta-analysis was performe...
Source: Braz J Med Biol Res - May 20, 2020 Category: Research Authors: Qin B, Yang MX, Gao W, Zhang JD, Zhao LB, Qin HX, Chen H Tags: Braz J Med Biol Res Source Type: research

Notoginsenoside R1 upregulates miR-221-3p expression to alleviate ox-LDL-induced apoptosis, inflammation, and oxidative stress by inhibiting the TLR4/NF- κB pathway in HUVECs.
This study aimed to explore the mechanism of NGR1 for treating AS through miRNAs. Flow cytometry was used to detect the apoptosis rate. The levels of inflammatory cytokines interleukin (IL)-6 and IL-1β were detected using ELISA. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels were measured using corresponding assay kits. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed to detect miR-221-3p expression. Dual-luciferase reporter and RNA immunoprecipitation assays were carried out to examine the relationship between miR-221-3p and toll-like receptors 4 (TLR4). Also, western blo...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Zhu L, Gong X, Gong J, Xuan Y, Fu T, Ni S, Xu L, Ji N Tags: Braz J Med Biol Res Source Type: research

High levels of chemerin associated with variants in the NOS3 and APOB genes in rural populations of Ouro Preto, Minas Gerais, Brazil.
GLL Abstract Chemerin is an adipokine that has been associated with components of metabolic syndrome. It has been described to affect adipocyte metabolism and inflammatory responses in adipose tissue, as well as the systemic metabolism of lipids and glucose. Few epidemiological studies have evaluated classical and genetics cardiovascular risk factors (CVRFs) in the mixed adult rural population in Brazil. Therefore, the present study explored possible associations between CVRFs and chemerin. This cross-sectional study included 508 adults from the rural localities of Lavras Novas, Chapada, and Santo Antônio d...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Batista AP, Barbosa KF, Masioli CZ, Queiroz EM, Marinho CC, Cândido APC, Machado-Coelho GLL Tags: Braz J Med Biol Res Source Type: research