Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis
Braz J Med Biol Res. 2024 Mar 18;57:e12951. doi: 10.1590/1414-431X2024e12951. eCollection 2024.ABSTRACTThe incidences of periodontitis and osteoporosis are rising worldwide. Observational studies have shown that periodontitis is associated with increased risk of osteoporosis. We performed a Mendelian randomization (MR) study to genetically investigate the causality of periodontitis on osteoporosis. We explored the causal effect of periodontitis on osteoporosis by MR analysis. A total of 9 single nucleotide polymorphisms (SNP) were related to periodontitis. The primary approach in this MR analysis was the inverse variance-w...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jiale Wu Lihui Yao Yuchen Liu ShuaiShuai Zhang Kan Wang Source Type: research

Temporal evolution of postsurgical bone repair in a rabbit model: A [99mTc]Tc-MDP scintigraphic study
This study used bone scintigraphy to evaluate osteogenesis secondary to osteotomy in a preclinical model of New Zealand rabbits. For this purpose, we conducted a longitudinal, prospective, case-control study in which scintigraphic variables were measured in both the right forearm (case-operated) and the left forearm (control - non-operated). The study sample consisted of 10 rabbits subjected to osteotomy, followed by a 12-week postoperative evaluation period, divided into six imaging stages at 1, 2, 3, 4, 8, and 12 weeks. We observed that the operated forearm showed significantly higher external radiation than the control ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: A Yoneda K J C C de Lacerda L Alexandre-Santos E N Itikawa P Louzada-Junior L Wichert-Ana Source Type: research

Serum autophagy-related gene 5 level in stroke patients: correlation with CD4+ T cells and cognition impairment during a 3-year follow-up
This study aimed to explore the correlation of serum ATG5 with CD4+ T cells and cognition impairment in stroke patients. Peripheral blood was collected from 180 stroke patients for serum ATG5 and T helper (Th) 1, Th2, Th17, and regulatory T (Treg) cell detection via enzyme-linked immunosorbent assays and flow cytometry. The Mini-Mental State Examination (MMSE) scale was completed at enrollment, year (Y)1, Y2, and Y3 in stroke patients. Serum ATG5 was also measured in 50 healthy controls (HCs). Serum ATG5 was elevated in stroke patients compared to HCs (P<0.001) and was positively correlated to Th2 cells (P=0.022), Th17 ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Juanjuan Qu Linxia Wu Meng Zhang Minchen Kan Huimin Chen Yanqing Shi Shuangyu Wang Xiaohua Wang Fan Chen Source Type: research

Upregulation of PGC-1 α expression by pioglitazone mediates prevention of sepsis-induced acute lung injury
This study aimed to investigate the role of the PGC-1α agonist pioglitazone (PGZ) in modulating sepsis-induced ALI. A mouse model of sepsis-induced ALI was established using cecal ligation and puncture (CLP). An in vitro model was created by stimulating MH-S cells with lipopolysaccharide (LPS). qRT-PCR was used to measure mRNA levels of M1 markers iNOS and MHC-II and M2 markers Arg1 and CD206 to evaluate macrophage polarization. Western blotting detected expression of peroxisome proliferator-activated receptor gamma (PPARγ) PGC-1α, and mitochondrial biogenesis proteins NRF1, NRF2, and mtTFA. To assess mitochondrial cont...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jing Tang Wenzhu Dong Dan Wang Qin Deng Honggang Guo Guibao Xiao Source Type: research

Silencing of matrix metalloprotease-12 delays the progression of castration-resistant prostate cancer by regulating autophagy and lipolysis
In this study, we observed the effect of MMP-12 on the progression of CRPC and further explored its potential mechanism of action. High levels of MMP-12 were observed in patients with CRPC. We therefore developed cell co-culture and mouse models to study the function of MMP-12. Silencing MMP-12 in CRPC cells disrupted lipid utilization and autophagy marker expression via the CD36/CPT1 and P62/LC3 pathways, respectively, leading to reduced CRPC cell migration and invasion. Moreover, animal experiments confirmed that MMP-12-knockdown CRPC xenograft tumors exhibited reduced tumor growth, and the mechanisms involved the promot...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Xiaoyu Zheng Xiaoqin Xie Wei Wang Liang Wang Bing Tan Source Type: research

Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis
Braz J Med Biol Res. 2024 Mar 18;57:e12951. doi: 10.1590/1414-431X2024e12951. eCollection 2024.ABSTRACTThe incidences of periodontitis and osteoporosis are rising worldwide. Observational studies have shown that periodontitis is associated with increased risk of osteoporosis. We performed a Mendelian randomization (MR) study to genetically investigate the causality of periodontitis on osteoporosis. We explored the causal effect of periodontitis on osteoporosis by MR analysis. A total of 9 single nucleotide polymorphisms (SNP) were related to periodontitis. The primary approach in this MR analysis was the inverse variance-w...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jiale Wu Lihui Yao Yuchen Liu ShuaiShuai Zhang Kan Wang Source Type: research

Temporal evolution of postsurgical bone repair in a rabbit model: A [99mTc]Tc-MDP scintigraphic study
This study used bone scintigraphy to evaluate osteogenesis secondary to osteotomy in a preclinical model of New Zealand rabbits. For this purpose, we conducted a longitudinal, prospective, case-control study in which scintigraphic variables were measured in both the right forearm (case-operated) and the left forearm (control - non-operated). The study sample consisted of 10 rabbits subjected to osteotomy, followed by a 12-week postoperative evaluation period, divided into six imaging stages at 1, 2, 3, 4, 8, and 12 weeks. We observed that the operated forearm showed significantly higher external radiation than the control ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: A Yoneda K J C C de Lacerda L Alexandre-Santos E N Itikawa P Louzada-Junior L Wichert-Ana Source Type: research

Serum autophagy-related gene 5 level in stroke patients: correlation with CD4+ T cells and cognition impairment during a 3-year follow-up
This study aimed to explore the correlation of serum ATG5 with CD4+ T cells and cognition impairment in stroke patients. Peripheral blood was collected from 180 stroke patients for serum ATG5 and T helper (Th) 1, Th2, Th17, and regulatory T (Treg) cell detection via enzyme-linked immunosorbent assays and flow cytometry. The Mini-Mental State Examination (MMSE) scale was completed at enrollment, year (Y)1, Y2, and Y3 in stroke patients. Serum ATG5 was also measured in 50 healthy controls (HCs). Serum ATG5 was elevated in stroke patients compared to HCs (P<0.001) and was positively correlated to Th2 cells (P=0.022), Th17 ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Juanjuan Qu Linxia Wu Meng Zhang Minchen Kan Huimin Chen Yanqing Shi Shuangyu Wang Xiaohua Wang Fan Chen Source Type: research

Upregulation of PGC-1 α expression by pioglitazone mediates prevention of sepsis-induced acute lung injury
This study aimed to investigate the role of the PGC-1α agonist pioglitazone (PGZ) in modulating sepsis-induced ALI. A mouse model of sepsis-induced ALI was established using cecal ligation and puncture (CLP). An in vitro model was created by stimulating MH-S cells with lipopolysaccharide (LPS). qRT-PCR was used to measure mRNA levels of M1 markers iNOS and MHC-II and M2 markers Arg1 and CD206 to evaluate macrophage polarization. Western blotting detected expression of peroxisome proliferator-activated receptor gamma (PPARγ) PGC-1α, and mitochondrial biogenesis proteins NRF1, NRF2, and mtTFA. To assess mitochondrial cont...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jing Tang Wenzhu Dong Dan Wang Qin Deng Honggang Guo Guibao Xiao Source Type: research

Silencing of matrix metalloprotease-12 delays the progression of castration-resistant prostate cancer by regulating autophagy and lipolysis
In this study, we observed the effect of MMP-12 on the progression of CRPC and further explored its potential mechanism of action. High levels of MMP-12 were observed in patients with CRPC. We therefore developed cell co-culture and mouse models to study the function of MMP-12. Silencing MMP-12 in CRPC cells disrupted lipid utilization and autophagy marker expression via the CD36/CPT1 and P62/LC3 pathways, respectively, leading to reduced CRPC cell migration and invasion. Moreover, animal experiments confirmed that MMP-12-knockdown CRPC xenograft tumors exhibited reduced tumor growth, and the mechanisms involved the promot...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Xiaoyu Zheng Xiaoqin Xie Wei Wang Liang Wang Bing Tan Source Type: research

Periodontitis and osteoporosis: a two-sample Mendelian randomization analysis
Braz J Med Biol Res. 2024 Mar 18;57:e12951. doi: 10.1590/1414-431X2024e12951. eCollection 2024.ABSTRACTThe incidences of periodontitis and osteoporosis are rising worldwide. Observational studies have shown that periodontitis is associated with increased risk of osteoporosis. We performed a Mendelian randomization (MR) study to genetically investigate the causality of periodontitis on osteoporosis. We explored the causal effect of periodontitis on osteoporosis by MR analysis. A total of 9 single nucleotide polymorphisms (SNP) were related to periodontitis. The primary approach in this MR analysis was the inverse variance-w...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jiale Wu Lihui Yao Yuchen Liu ShuaiShuai Zhang Kan Wang Source Type: research

Temporal evolution of postsurgical bone repair in a rabbit model: A [99mTc]Tc-MDP scintigraphic study
This study used bone scintigraphy to evaluate osteogenesis secondary to osteotomy in a preclinical model of New Zealand rabbits. For this purpose, we conducted a longitudinal, prospective, case-control study in which scintigraphic variables were measured in both the right forearm (case-operated) and the left forearm (control - non-operated). The study sample consisted of 10 rabbits subjected to osteotomy, followed by a 12-week postoperative evaluation period, divided into six imaging stages at 1, 2, 3, 4, 8, and 12 weeks. We observed that the operated forearm showed significantly higher external radiation than the control ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: A Yoneda K J C C de Lacerda L Alexandre-Santos E N Itikawa P Louzada-Junior L Wichert-Ana Source Type: research

Serum autophagy-related gene 5 level in stroke patients: correlation with CD4+ T cells and cognition impairment during a 3-year follow-up
This study aimed to explore the correlation of serum ATG5 with CD4+ T cells and cognition impairment in stroke patients. Peripheral blood was collected from 180 stroke patients for serum ATG5 and T helper (Th) 1, Th2, Th17, and regulatory T (Treg) cell detection via enzyme-linked immunosorbent assays and flow cytometry. The Mini-Mental State Examination (MMSE) scale was completed at enrollment, year (Y)1, Y2, and Y3 in stroke patients. Serum ATG5 was also measured in 50 healthy controls (HCs). Serum ATG5 was elevated in stroke patients compared to HCs (P<0.001) and was positively correlated to Th2 cells (P=0.022), Th17 ...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Juanjuan Qu Linxia Wu Meng Zhang Minchen Kan Huimin Chen Yanqing Shi Shuangyu Wang Xiaohua Wang Fan Chen Source Type: research

Upregulation of PGC-1 α expression by pioglitazone mediates prevention of sepsis-induced acute lung injury
This study aimed to investigate the role of the PGC-1α agonist pioglitazone (PGZ) in modulating sepsis-induced ALI. A mouse model of sepsis-induced ALI was established using cecal ligation and puncture (CLP). An in vitro model was created by stimulating MH-S cells with lipopolysaccharide (LPS). qRT-PCR was used to measure mRNA levels of M1 markers iNOS and MHC-II and M2 markers Arg1 and CD206 to evaluate macrophage polarization. Western blotting detected expression of peroxisome proliferator-activated receptor gamma (PPARγ) PGC-1α, and mitochondrial biogenesis proteins NRF1, NRF2, and mtTFA. To assess mitochondrial cont...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Jing Tang Wenzhu Dong Dan Wang Qin Deng Honggang Guo Guibao Xiao Source Type: research

Silencing of matrix metalloprotease-12 delays the progression of castration-resistant prostate cancer by regulating autophagy and lipolysis
In this study, we observed the effect of MMP-12 on the progression of CRPC and further explored its potential mechanism of action. High levels of MMP-12 were observed in patients with CRPC. We therefore developed cell co-culture and mouse models to study the function of MMP-12. Silencing MMP-12 in CRPC cells disrupted lipid utilization and autophagy marker expression via the CD36/CPT1 and P62/LC3 pathways, respectively, leading to reduced CRPC cell migration and invasion. Moreover, animal experiments confirmed that MMP-12-knockdown CRPC xenograft tumors exhibited reduced tumor growth, and the mechanisms involved the promot...
Source: Braz J Med Biol Res - March 21, 2024 Category: Research Authors: Xiaoyu Zheng Xiaoqin Xie Wei Wang Liang Wang Bing Tan Source Type: research