Phenotypic changes of interstitial cells of Cajal after intestinal obstruction in rat model.
The objective was to study the effect of mechanical intestinal obstruction in rats on the phenotype of interstitial cells of Cajal (ICC). Healthy Wistar rats were randomly divided into sham-operation group (C), one day obstruction group (M1), two days obstruction group (M2), and three days obstruction group (M3), with 10 rats in each group. The expression of SCF mRNA and c-Kit protein in intestinal tissue was investigated by RT-PCR and immunohistochemistry. Compared with the sham-operation group, the relative expression of SCF mRNA and the expression of c-Kit protein in intestinal tissue were significantly decreased in bot...
Source: Braz J Med Biol Res - October 18, 2019 Category: Research Authors: Li L, Zou C, Zhou Z, Wang X, Yu X Tags: Braz J Med Biol Res Source Type: research

Higher values of triglycerides:HDL-cholesterol ratio hallmark disease severity in children and adolescents with sickle cell anemia.
Abstract Dyslipidemia has been described in sickle cell anemia (SCA) but its association with increased disease severity is unknown. Here, we examined 55 children and adolescents with SCA as well as 41 healthy controls to test the association between the lipid profiles in peripheral blood and markers of hemolysis, inflammation, endothelial function, and SCA-related clinical outcomes. SCA patients exhibited lower levels of total cholesterol (P
Source: Braz J Med Biol Res - October 18, 2019 Category: Research Authors: Teixeira RS, Arriaga MB, Terse-Ramos R, Ferreira TA, Machado VR, Rissatto-Lago MR, Silveira-Mattos PS, Boa-Sorte N, Ladeia AMT, Andrade BB Tags: Braz J Med Biol Res Source Type: research

Schizandrin A exerts anti-tumor effects on A375 cells by down-regulating H19.
Abstract Malignant melanoma (MM) is one of the malignant tumors with highly metastatic and aggressive biological actions. Schizandrin A (SchA) is a bioactive lignin compound with strong anti-oxidant and anti-aging properties, which is stable at room temperature and is often stored in a cool dry place. Hence, we investigated the effects of SchA on MM cell line A375 and its underlying mechanism. A375 cells were used to construct an in vitro MM cell model. Cell viability, proliferation, apoptosis, and migration were detected by Cell Counting Kit-8, BrdU assay, flow cytometry, and transwell two-chamber assay, respecti...
Source: Braz J Med Biol Res - October 18, 2019 Category: Research Authors: Bi Y, Fu Y, Wang S, Chen X, Cai X Tags: Braz J Med Biol Res Source Type: research

Propranolol inhibits myocardial infarction-induced brown adipose tissue D2 activation and maintains a low thyroid hormone state in rats.
es EL Abstract Considering the recognized role of thyroid hormones on the cardiovascular system during health and disease, we hypothesized that type 2 deiodinase (D2) activity, the main activation pathway of thyroxine (T4)-to-triiodothyronine (T3), could be an important site to modulate thyroid hormone status, which would then constitute a possible target for β-adrenergic blocking agents in a myocardial infarction (MI) model induced by left coronary occlusion in rats. Despite a sustained and dramatic fall in serum T4 concentrations (60-70%), the serum T3 concentration fell only transiently in the first week p...
Source: Braz J Med Biol Res - October 18, 2019 Category: Research Authors: Seara FAC, Araujo IG, Império GE, Marassi MP, Silva ACM, Mecawi AS, Reis LC, Olivares EL Tags: Braz J Med Biol Res Source Type: research

Lichen planus with multiple system involvement including the mouth, vagina, urethra, and scalp: a case report.
Abstract This is a case report of lichen planus (LP) with multiple system involvement. A 35-year-old female patient was admitted in November 2014 with a 5-year history of painful/difficult sexual intercourse and loss of oral mucosa, and an 8-year history of focal hair loss. Earlier, the patient had been unable to adhere to corticosteroid therapy because of severe adverse side effects. In September 2014, labia minora mucosa defects and stricture of the urethral orifice (with dysuria), vaginal orifice, and vagina were identified. Biopsy was performed and a diagnosis of erosive LP was made. The patient was treated wi...
Source: Braz J Med Biol Res - October 18, 2019 Category: Research Authors: Mi L, Zhang H, Zhang D, Zhang M Tags: Braz J Med Biol Res Source Type: research

Hypogammaglobulinemia: a diagnosis that must not be overlooked.
Abstract Humoral immunological defects are frequent and important causes of hypogammaglobulinemia, leading to recurrent infections, autoimmunity, allergies, and neoplasias. Usually, its onset occurs in childhood or during the second and third decades of life; however, the diagnosis is made, on average, 6 to 7 years afterwards. As a consequence, antibody defects can lead to sequelae. Here we describe the clinical-laboratory characteristics, treatment, and prognoses of patients with hypogammaglobulinemia. An observational, cross-sectional, and retrospective study of patients attending the recently established outpat...
Source: Braz J Med Biol Res - October 18, 2019 Category: Research Authors: Pimenta FMCA, Palma SMU, Constantino-Silva RN, Grumach AS Tags: Braz J Med Biol Res Source Type: research

The role of KDR in intrauterine adhesions may involve the TGF- β1/Smads signaling pathway.
The role of KDR in intrauterine adhesions may involve the TGF-β1/Smads signaling pathway. Braz J Med Biol Res. 2019;52(10):e8324 Authors: Chen JX, Yi XJ, Gu PL, Gao SX Abstract The aim of this study was to investigate the role of kinase-insert domain-containing receptor (KDR) in intrauterine adhesions (IUA) and its mechanism. The Case group consisted of 92 patients diagnosed with IUA, and the Control group included 86 patients with uterine septum who had normal endometrium verified with an uteroscope. In addition, 50 rats were randomly assigned into Control, Sham, Model, NC-siRNA, and KDR-siRNA g...
Source: Braz J Med Biol Res - October 11, 2019 Category: Research Authors: Chen JX, Yi XJ, Gu PL, Gao SX Tags: Braz J Med Biol Res Source Type: research

Short-term creatine supplementation changes protein metabolism signaling in hindlimb suspension.
In conclusion, short-term creatine supplementation changed protein metabolism signaling in soleus and EDL muscles. However, creatine supplementation only slightly attenuated the mass loss of both muscles and did not prevent the CSA reduction and muscle strength decrease induced by HS for 5 days. PMID: 31596311 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - October 11, 2019 Category: Research Authors: Marzuca-Nassr GN, Fortes MAS, Guimarães-Ferreira L, Murata GM, Vitzel KF, Vasconcelos DAA, Bassit RA, Curi R Tags: Braz J Med Biol Res Source Type: research

Serum regucalcin is a useful indicator of liver injury severity in patients with hepatitis B virus-related liver diseases.
Abstract Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal antibodies and a polyclonal antibody to regucalcin were produced, a double-antibody sandwich ELISA method was established, and serum regucalcin was determined in 47 chronic hepatitis B (CHB) patients, 91 HBV-related acute-on-chronic liver failure (HBV-AC...
Source: Braz J Med Biol Res - October 4, 2019 Category: Research Authors: Wei X, Yu H, Zhao P, Xie L, Li L, Zhang J Tags: Braz J Med Biol Res Source Type: research

Impact of body characteristics on ultrasound-measured inferior vena cava parameters in Chinese children.
In conclusion, IVCmax and Ao were more susceptible to subjects' characteristics than IVCmax/Ao. IVCmax/Ao could be a reliable and practical parameter in Chinese children as it was independent of age, height, and weight. PMID: 31531523 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - September 19, 2019 Category: Research Authors: Gui J, Zhou B, Liu J, Ou B, Wang Y, Jiang L, Tang W, Luo B, Yang Z Tags: Braz J Med Biol Res Source Type: research

Small RNA sequencing reveals microRNAs related to neuropathic pain in rats.
Abstract The present study aimed to identify microRNAs (miRNAs) that are involved in neuropathic pain and predict their corresponding roles in the pathogenesis and development process of neuropathic pain. The rat model of neuropathic pain caused by spared nerve injury (SNI) was established in Sprague-Dawley male rats, followed by small RNA sequencing of the L3-L6 dorsal root ganglion. Real-time PCR was performed to validate the differently expressed miRNAs. Functional verification was performed by intrathecally injecting the animals with miRNA agomir. A total of 72 differentially expressed miRNAs were identified i...
Source: Braz J Med Biol Res - September 19, 2019 Category: Research Authors: Dai D, Wang J, Jiang Y, Yuan L, Lu Y, Zhang A, Zou D, Chen X Tags: Braz J Med Biol Res Source Type: research

Clinical efficacy of tirofiban combined with a Solitaire stent in treating acute ischemic stroke.
This study explores the safety and effect of acute cerebral infarction treatment by microcatheter injection of tirofiban combined with a Solitaire AB stent and/or stent implantation. Emergency cerebral angiograms showing the responsible vascular occlusion of 120 acute cerebral infarction patients who underwent emergency endovascular thrombectomy were included in the study. These patients were randomly divided into two groups using the random number table method: treatment group (n=60) that received thrombectomy (with cerebral artery stents) combined with intracerebral injection of tirofiban and control group (n=60) that on...
Source: Braz J Med Biol Res - September 19, 2019 Category: Research Authors: Zhang Y, Zhang QQ, Fu C, Wang L, Zhang GQ, Cao PW, Chen GF, Fu XM Tags: Braz J Med Biol Res Source Type: research

lncRNA MEG3 inhibits the growth of hepatocellular carcinoma cells by sponging miR-9-5p to upregulate SOX11.
Abstract The long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3), a tumor suppressor, is critical for the carcinogenesis and progression of different cancers, including hepatocellular carcinoma (HCC). To date, the roles of lncRNA MEG3 in HCC are not well illustrated. Therefore, this study used western blot and qRT-PCR to evaluate the expression of MEG3, miR-9-5p, and Sex determining Region Y-related HMG-box 11 (SOX11) in HCC tissues and cell lines. RNA pull-down and luciferase reporter assay were used to evaluate these molecular interactions. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide...
Source: Braz J Med Biol Res - September 19, 2019 Category: Research Authors: Liu Z, Chen JY, Zhong Y, Xie L, Li JS Tags: Braz J Med Biol Res Source Type: research

Presence of age- and sex-related differences in heart rate variability despite the maintenance of a suitable level of accelerometer-based physical activity.
Abstract There is currently a lack of information adjacent on the influence of sex and age in heart rate variability (HRV), adjusted according to accelerometer-based physical activity (PADL). We hypothesized that the effect of sex and age on the HRV should be reduced or absent in individuals with a suitable PADL level. We aim to evaluate the influence of sex and age on HRV, adjusted for the confounding effects of the PADL level. A total of 485 age-stratified subjects (18-39, 40-59, and ≥60 years) underwent HRV analyses at rest and 7-day assessments of accelerometer-based PADL. Multivariate analyses of covarianc...
Source: Braz J Med Biol Res - August 9, 2019 Category: Research Authors: Spina GD, Gonze BB, Barbosa ACB, Sperandio EF, Dourado VZ Tags: Braz J Med Biol Res Source Type: research

Recurrent hepatitis C treatment with direct acting antivirals - a real life study at a Brazilian liver transplant center.
i RSB Abstract Recurrent hepatitis C (HCV) after liver transplantation (LT) is an important cause of morbidity and mortality. Antiviral treatment is recommended to avoid unfavorable outcomes. Direct-acting antivirals (DAA) have transformed HCV treatment, with higher efficacy and fewer side-effects than interferon-based therapies traditionally used. To evaluate DAA treatment outcomes at a Brazilian transplant unit, data of patients who finished HCV treatment at the Liver Transplant Unit of the University of Campinas were analyzed. Treatment consisted of sofosbuvir, daclatasvir, and ribavirin, for 12 or 24 weeks, ac...
Source: Braz J Med Biol Res - August 9, 2019 Category: Research Authors: Zanaga LP, Santos AG, Ataíde EC, Boin IFSF, Stucchi RSB Tags: Braz J Med Biol Res Source Type: research

Comparison of three PET methods to assess peritoneal membrane transport.
This study compared three methods: traditional PET (t-PET), mini-PET, and modified PET (mod-PET). Non-diabetic adults (n=21) who had been on peritoneal dialysis (PD) for at least three months underwent t-PET (glucose 2.5%-4 h), mini-PET (glucose 3.86%-1 h), and mod-PET (glucose 3.86%-4 h) to determine dialysate-to-plasma concentration ratio (D/P) for creatinine and dialysate-to-baseline dialysate concentration ratio (D/D0) for glucose. Agreement between methods regarding D/P creatinine and D/D0 glucose was assessed using analysis of variance (ANOVA), Pearson's correlation coefficient, and Bland-Altman analysis. D/P creatin...
Source: Braz J Med Biol Res - August 9, 2019 Category: Research Authors: Romani RF, Waniewski J, Kruger L, Lindholm B, Nascimento MM Tags: Braz J Med Biol Res Source Type: research

Decreased respiratory performance of children and adolescents with myelomeningocele who use a wheelchair - preliminary data.
Abstract Myelomeningocele (MMC) is a neural tube defect that often causes spinal cord injury at the thoracolumbar region, as well as sensory and motor paralysis in the lower limbs. This leads to continuous use of a wheelchair and, consequently, a sedentary lifestyle, predisposition to muscle weakness, cardiovascular and respiratory disorders, obesity, and structural alterations in the spine. We assessed the respiratory function and shoulder strength of MMC participants who were wheelchair-users and had no respiratory complaints and compared them to healthy children and adolescents. MMC (n=10) and healthy (n=25) pa...
Source: Braz J Med Biol Res - August 9, 2019 Category: Research Authors: Martins EJ, Gastaldi AC, Davoli GBQ, Leonardi-Figueiredo MM, Mattiello-Sverzut AC Tags: Braz J Med Biol Res Source Type: research

Does whole body vibration exercise improve oxidative stress markers in women with fibromyalgia?
The objective of this study was to investigate the effect of whole body vibration (WBV) exercise on oxidative stress markers in a group of women with fibromyalgia (FM) compared to a group of healthy women (CT). Twenty-one women diagnosed with FM and 21 age- and weight-matched healthy women were enrolled the study. Plasma oxidative stress markers (primary outcomes) were evaluated at rest and after WBV, and included thiobarbituric acid reactive substances (TBARS), iron reduction capacity (FRAP), superoxide dismutase antioxidant enzymes activity (SOD), and catalase (CAT). At rest, the FM group had higher TBARS (P
Source: Braz J Med Biol Res - August 9, 2019 Category: Research Authors: Santos JM, Mendonça VA, Ribeiro VGC, Tossige-Gomes R, Fonseca SF, Prates ACN, Flor J, Oliveira ACC, Martins JB, Garcia BCC, Leite HR, Figueiredo PHS, Bernardo-Filho M, Lacerda ACR Tags: Braz J Med Biol Res Source Type: research

Reproducibility of carotid ultrasound measurements in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) at baseline.
In conclusion, we found very good reproducibility for carotid plaque score and CIMT measurements in the ELSA-Brasil at baseline. These results are comparable to the best findings from similar large cohorts that analyzed carotid ultrasound data. PMID: 31389494 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - August 9, 2019 Category: Research Authors: Santos-Neto PJ, Sena-Santos EH, Meireles DP, Santos IS, Bensenor IM, Lotufo PA Tags: Braz J Med Biol Res Source Type: research

MicroRNA-383-5p inhibits the progression of gastric carcinoma via targeting HDAC9 expression.
Abstract MicroRNAs (miRNAs), as post-transcriptional regulators, have been reported to be involved in the initiation and progression of various types of cancer, including gastric cancer (GC). The present study aimed to investigate the role of miR-383-5p in gastric carcinogenesis. Cell viability was analyzed using CCK-8 kit. Annexin V-fluorescein isothiocyanate/propidium iodide double staining was used to evaluate cell apoptosis. The expression levels of miR-383-5p and histone deacetylase 9 (HDAC9) mRNA in GC tissues and cell lines were analyzed using RT-qPCR. The protein expression of HDAC9 was detected by western...
Source: Braz J Med Biol Res - August 2, 2019 Category: Research Authors: Xu G, Li N, Zhang Y, Zhang J, Xu R, Wu Y Tags: Braz J Med Biol Res Source Type: research

Association between BDNF G196A (Val66Met) polymorphism and cognitive impairment in patients with Parkinson's disease: a meta-analysis.
In conclusion, BDNF G196A (Val66Met) is confirmed to be a risk factor for cognitive impairment in PD. PMID: 31365694 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - August 2, 2019 Category: Research Authors: Wang Q, Liu J, Guo Y, Dong G, Zou W, Chen Z Tags: Braz J Med Biol Res Source Type: research

Ventilation distribution as a contributor to the functional exercise capacity in patients with systemic sclerosis-associated interstitial lung disease without pulmonary hypertension.
Abstract Phenotypic differences have been described between patients with systemic sclerosis (SSc)-associated interstitial lung disease (ILD) and SSc-associated pulmonary hypertension, including performance differences in the 6-min walk test (6MWT). Moreover, the correlations between the 6MWT and traditional pulmonary function tests (PFTs) are weak, indicating the need to search for new parameters that explain exercise performance. Thus, our objective was to evaluate the impact of ventilation distribution heterogeneity assessed by the nitrogen single-breath washout (N2SBW) test and peripheral muscle dysfunction on...
Source: Braz J Med Biol Res - August 2, 2019 Category: Research Authors: Andrade FM, Oliveira AD, Lopes AJ Tags: Braz J Med Biol Res Source Type: research

Gene expression profiling reveals upregulated FUT1 and MYBPC1 in children with pancreaticobiliary maljunction.
Abstract Pancreaticobiliary maljunction (PBM) is associated with high risk of epithelial atypical growth and malignant transformation of the bile duct or gallbladder. However, overall changes in genetic expression have not been examined in children with PBM. Genome-wide expression was analyzed using peripheral blood samples from 10 children with PBM and 15 pediatric controls. Differentially expressed genes (DEGs) were identified using microarray. Bioinformatics analysis was conducted using Gene Ontology and KEGG analyses. The top 5 in the up-regulated genes in PBM were verified with qRT-PCR. Receiver operator char...
Source: Braz J Med Biol Res - August 2, 2019 Category: Research Authors: Guo WL, Geng J, Zhao JG, Fang F, Huang SG, Wang J Tags: Braz J Med Biol Res Source Type: research

Association between elevated adiponectin level and adverse outcomes in patients with heart failure: a systematic review and meta-analysis.
Abstract Studies regarding the prognostic value of circulating adiponectin level in patients with heart failure are conflicting. The aim of this meta-analysis was to evaluate the association between elevated circulating adiponectin level and adverse outcomes in patients with heart failure. We searched PubMed and Embase databases from their inception to June 2018. Original observational studies that investigated the prognostic value of adiponectin in heart failure patients and reported all-cause mortality or combined endpoints of death/readmission as outcome measure were included. Pooled risk ratio (RR) with 95% co...
Source: Braz J Med Biol Res - July 19, 2019 Category: Research Authors: Bai W, Huang J, Zhu M, Liu X, Tao J Tags: Braz J Med Biol Res Source Type: research

Age-related changes and effects of regular low-intensity exercise on gait, balance, and oxidative biomarkers in the spinal cord of Wistar rats.
Abstract The present study aimed to analyze age-related changes to motor coordination, balance, spinal cord oxidative biomarkers in 3-, 6-, 18-, 24-, and 30-month-old rats. The effects of low-intensity exercise on these parameters were also analyzed in 6-, 18-, and 24-month-old rats. Body weight, blood glucose, total cholesterol, and high-density lipoprotein (HDL) cholesterol were assessed for all rats. The soleus muscle weight/body weight ratio was used to estimate skeletal muscle mass loss. Body weight increased until 24 months; only 30-month-old rats exhibited decreased blood glucose and increased total cholest...
Source: Braz J Med Biol Res - July 19, 2019 Category: Research Authors: Silveira EMS, Kroth A, Santos MCQ, Silva TCB, Silveira D, Riffel APK, Scheid T, Trapp M, Partata WA Tags: Braz J Med Biol Res Source Type: research

Carotid intima-media thickness and plaques in internal carotid artery as surrogate markers of lower limb arterial lesions in Chinese patients with diabetic foot.
Abstract Peripheral arterial disease (PAD) can impair healing of diabetic foot (DF) in patients with diabetes mellitus. To determine whether carotid intima-media thickness (CIMT) can predict lower limb arterial lesions in patients with DF, this cross-sectional study enrolled patients with DF at West China Hospital (China) between January 2012 and December 2015. Ultrasonography was used to measure CIMT, assess the internal carotid arteries (ICA) for plaques, and evaluate lower limb segmental arteries for stenosis. The optimal CIMT cutoff for detecting lower limb PAD was determined by receiver operating characterist...
Source: Braz J Med Biol Res - July 19, 2019 Category: Research Authors: Zhang M, Wen X, Zhou C, Huang J, He Y Tags: Braz J Med Biol Res Source Type: research

Analysis of different levels of positive end-expiratory pressure during lung retrieval for transplantation: an experimental study.
oa JM Abstract Atelectasis and inadequate oxygenation in lung donors is a common problem during the retrieval of these organs. Nevertheless, the use of high positive end-expiratory pressure (PEEP) is not habitual during procedures of lung retrieval. Twenty-one Sprague-Dawley male consanguineous rats were used in the study. The animals were divided into 3 groups according to the level of PEEP used: low (2 cmH2O), moderate (5 cmH2O), and high (10 cmH2O). Animals were ventilated with a tidal volume of 6 mL/kg. Before lung removal, the lungs were inspected for the presence of atelectasis. When atelectasis was detected...
Source: Braz J Med Biol Res - July 19, 2019 Category: Research Authors: Gonçalves-Ferri WA, Jauregui A, Martins-Celini FP, Sansano I, Fabro AT, Sacramento EMF, Aragon DC, Ochoa JM Tags: Braz J Med Biol Res Source Type: research

Ca2+/Calmodulin-dependent kinase II delta B is essential for cardiomyocyte hypertrophy and complement gene expression after LPS and HSP60 stimulation in vitro.
This study evaluated mechanisms underlying inflammatory response and cardiomyocyte hypertrophy associated with bacterial lipopolysaccharide (LPS)- or heat shock protein 60 (HSP60)-induced Toll-like receptor (TLR) stimulation and the effect of a small interfering RNA (siRNA) against Ca2+/calmodulin-dependent kinase II delta B (CaMKIIδB) on these outcomes. Our results showed that treatment with HSP60 or LPS (TLR agonists) induced cardiomyocyte hypertrophy and complement system C3 and factor B gene expression. In vitro silencing of CaMKIIδB prevented complement gene transcription and cardiomyocyte hypertrophy asso...
Source: Braz J Med Biol Res - July 19, 2019 Category: Research Authors: Cruz Junho CV, Trentin-Sonoda M, Alvim JM, Gaisler-Silva F, Carneiro-Ramos MS Tags: Braz J Med Biol Res Source Type: research

Erratum for: "Effect of JJYMD-C, a novel synthetic derivative of gallic acid, on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro" [Braz J Med Biol Res (2014) 47(8): e3935].
Erratum for: "Effect of JJYMD-C, a novel synthetic derivative of gallic acid, on proliferation and phenotype maintenance in rabbit articular chondrocytes in vitro" [Braz J Med Biol Res (2014) 47(8): e3935]. Braz J Med Biol Res. 2019;52(7):e3935erratum Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20143935]. PMID: 31291380 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - July 11, 2019 Category: Research Tags: Braz J Med Biol Res Source Type: research

Henoch-Sch önlein purpura nephritis associated with monoclonal gammopathy of renal significance: a case report.
Henoch-Schönlein purpura nephritis associated with monoclonal gammopathy of renal significance: a case report. Braz J Med Biol Res. 2019;52(7):e8222 Authors: Zhao H, Huang WH, Huang JY, Lu SY, Yang YH, Ma ZG Abstract Monoclonal gammopathy of renal significance (MGRS) can present with different morphologic features and lead to kidney failure. The Henoch-Schönlein purpura nephritis (HSPN) that cannot be relieved by treatment with glucocorticoid and immunosuppressive agents suggests the presence of monoclonal gammopathy in adult patients. The present study reports on a single case of HSPN assoc...
Source: Braz J Med Biol Res - July 11, 2019 Category: Research Authors: Zhao H, Huang WH, Huang JY, Lu SY, Yang YH, Ma ZG Tags: Braz J Med Biol Res Source Type: research

Reinforcement omission effects in rats with bilateral lesions in the substantia nigra pars compacta and ventral tegmental area.
Abstract Reinforcement omission effects (ROEs) are characterized by higher response rates after reinforcement omission than after reinforcement delivery. This pattern of behavior is interpreted in terms of motivational and attentional processes. Recent studies from our laboratory have shown that the amygdala, nucleus accumbens, and medial prefrontal cortex are involved in ROE modulation. Also, the literature has demonstrated a role of other areas such as substantia nigra pars compacta (SNc) and the ventral tegmental area (VTA) in processes related to surprising events, such as prediction error and presentation or ...
Source: Braz J Med Biol Res - July 11, 2019 Category: Research Authors: Tavares TF, Judice-Daher DM, Bueno JLO Tags: Braz J Med Biol Res Source Type: research

Circulating high mobility group box-1 and toll-like receptor 4 expressions increase the risk and severity of epilepsy.
This study aimed to investigate the association of serum high-mobility group box-1 (HMGB1) and toll-like receptor 4 (TLR4) expressions with the risk of epilepsy as well as their correlations with disease severity and resistance to anti-epilepsy drugs. One hundred and five epilepsy patients and 100 healthy controls (HCs) were enrolled in this case-control study, and serum samples were collected from all participants to assess the HMGB1 and TLR4 expressions by enzyme-linked immunosorbent assay (ELISA). Both serum HMGB1 (P
Source: Braz J Med Biol Res - June 27, 2019 Category: Research Authors: Kan M, Song L, Zhang X, Zhang J, Fang P Tags: Braz J Med Biol Res Source Type: research

Epigallocatechin-3-gallate ameliorates lipopolysaccharide-induced acute lung injury by suppression of TLR4/NF- κB signaling activation.
Epigallocatechin-3-gallate ameliorates lipopolysaccharide-induced acute lung injury by suppression of TLR4/NF-κB signaling activation. Braz J Med Biol Res. 2019;52(7):e8092 Authors: Wang J, Fan SM, Zhang J Abstract Acute lung injury (ALI) is a serious clinical syndrome with a high rate of mortality. The activation of inflammation is well-recognized as a vital factor in the pathogenesis of lipopolysaccharide (LPS)-induced ALI. Therefore, suppression of the inflammatory response could be an ideal strategy to prevent ALI. Epigallocatechin-3-gallate (EGCG), mainly from green tea, has been shown to h...
Source: Braz J Med Biol Res - June 27, 2019 Category: Research Authors: Wang J, Fan SM, Zhang J Tags: Braz J Med Biol Res Source Type: research

T cell receptor excision circles as a tool for evaluating thymic function in young children.
Abstract The thymus is a primary lymphoid organ responsible for the maturation of T cells as well as the immunological central tolerance. It is in the antenatal period and infancy that it plays its major role. In clinical practice, T cell receptor excision circles (TRECs) are considered a direct and reliable measure of the thymic function. TRECs are a by-product of DNA formation in gene rearrangement of T cell receptors. They are stable and they do not duplicate during mitosis, representing the recent emigrant T cells from the thymus. Despite their importance, TRECs have been neglected by physicians and there is a...
Source: Braz J Med Biol Res - June 27, 2019 Category: Research Authors: Levy A, Rangel-Santos A, Torres LC, Silveira-Abreu G, Agena F, Carneiro-Sampaio M Tags: Braz J Med Biol Res Source Type: research

FSH receptor binding inhibitor up-regulates ARID1A and PTEN genes associated with ovarian cancers in mice.
Abstract Experiments were conducted to determine if the follicle-stimulating hormone (FSH) receptor binding inhibitor (FRBI) impacts the expression levels of AT-rich interactive domain-containing protein 1A (ARID1A) and phosphatase and tensin homolog (PTEN) in ovaries and blood, as well as expressions of follicle-stimulating hormone cognate receptor (FSHR) gene and proteins. Mice in FRBI-10, FRBI-20, FRBI-30, and FRBI-40 groups were intramuscularly injected with 10, 20, 30, and 40 mg FRBI/kg, respectively, for five consecutive days. Western blotting and qRT-PCR were utilized to determine expression levels of ARID1...
Source: Braz J Med Biol Res - June 27, 2019 Category: Research Authors: Gong Z, Shen X, Yang J, Yang K, Bai S, Wei S Tags: Braz J Med Biol Res Source Type: research

Baicalin ameliorates chronic mild stress-induced depression-like behaviors in mice and attenuates inflammatory cytokines and oxidative stress.
This study evaluated the behavioral and neuroprotective effects of BA in mice subjected to chronic unpredictable mild stress, a model of depression. BA (25 and 50 mg/kg) significantly increased sucrose consumption and reduced immobility times in the tail suspension and forced swim tests, demonstrating that BA alleviated depression-like behaviors. Moreover, BA reduced the levels of inflammatory cytokines, such as interleukin 1β, interleukin 6, and tumor necrosis factor α, in serum and in the hippocampus. BA also abrogated increases in NMDAR/NR2B and Ca2+/calmodulin-dependent protein kinase II, and the decrease in...
Source: Braz J Med Biol Res - June 27, 2019 Category: Research Authors: Zhong J, Li G, Xu H, Wang Y, Shi M Tags: Braz J Med Biol Res Source Type: research

Artesunate promotes Th1 differentiation from CD4+ T cells to enhance cell apoptosis in ovarian cancer via miR-142.
Abstract The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4+ T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4+ T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4+ T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-γ level was examined through enzyme-linked immunosorbent assay. Artesunate promoted mi...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Chen X, Zhang XL, Zhang GH, Gao YF Tags: Braz J Med Biol Res Source Type: research

A study on changes of the resting-state brain function network in patients with amnestic mild cognitive impairment.
This study aimed to explore the structural and functional characteristics of the neural network of resting-state brain activities in patients with amnestic mild cognitive impairment (aMCI) by functional magnetic resonance imaging (fMRI) technology. Resting state fMRI scanning was performed on 10 clinically diagnosed aMCI patients and 10 healthy volunteers, and the difference in the resting-state brain activities between aMCI patients and healthy volunteers was compared using the brain function network regional homogeneity (ReHo) analysis method. Results of the ReHo analysis of aMCI patients and healthy volunteers revealed ...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Min J, Zhou XX, Zhou F, Tan Y, Wang WD Tags: Braz J Med Biol Res Source Type: research

A biomechanical study on proximal junctional kyphosis following long-segment posterior spinal fusion.
This study was divided into two groups: non-fusion and fusion. In the former, the stress between the T10 and the upper thoracic vertebrae was higher. Comparing thoracic and lumbar segments in the fusion group, the peak stress values of the upper instrumented vertebrae (UIV) were mainly observed in T2 and L2 whilst those of the UIV+1 were observed in T10 and L2. After normalization, the peak stress values of the UIV and UIV+1 were located in T2 and L2. Similarly, the peak stress values of the annulus fibrosus at the upper adjacent level were on T10 and L2 after normalization. However, the peak stress values of the interspin...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Zhu WY, Zang L, Li J, Guan L, Hai Y Tags: Braz J Med Biol Res Source Type: research

Small-scale environmental enrichment and exercise enhance learning and spatial memory of Carassius auratus, and increase cell proliferation in the telencephalon: an exploratory study.
;o-Diniz CW Abstract Carassius auratus is a teleost fish that has been largely used in behavioral studies. However, little is known about potential environmental influences on its performance of learning and memory tasks. Here, we investigated this question in C. auratus, and searched for potential correlation between exercise and visuospatial enrichment with the total number of telencephalic glia and neurons. To that end, males and females were housed for 183 days in either an enriched (EE) or impoverished environment (IE) aquarium. EE contained toys, natural plants, and a 12-hour/day water stream for voluntary e...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Abreu CC, Fernandes TN, Henrique EP, Pereira PDC, Marques SB, Herdeiro SLS, Oliveira FRR, Magalhães NGM, Anthony DC, Melo MAD, Guerreiro-Diniz C, Diniz DG, Picanço-Diniz CW Tags: Braz J Med Biol Res Source Type: research

An update on the tools for creating transgenic animal models of human diseases - focus on atherosclerosis.
Abstract Animal models of diseases are invaluable tools of modern medicine. More than forty years have passed since the first successful experiments and the spectrum of available models, as well as the list of methods for creating them, have expanded dramatically. The major step forward in creating specific disease models was the development of gene editing techniques, which allowed for targeted modification of the animal's genome. In this review, we discuss the available tools for creating transgenic animal models, such as transgenesis methods, recombinases, and nucleases, including zinc finger nuclease (ZFN), tr...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Volobueva AS, Orekhov AN, Deykin AV Tags: Braz J Med Biol Res Source Type: research

Biomass smoke COPD has less tomographic abnormalities but worse hypoxemia compared with tobacco COPD.
Abstract Special attention has emerged towards biomass smoke-induced chronic obstructive pulmonary disease (COPD), providing new knowledge for prevention and therapeutic approach of non-smoker COPD patients. However, the understanding of biomass smoke COPD is still limited and somewhat controversial. The aim of the present study was to compare COPD exclusively caused by tobacco smoking with COPD exclusively caused by environmental or occupational exposures. For this cross-sectional study, COPD patients were recruited from outpatient clinics and formed two groups: non-smoker COPD group (n=16) with exposure to bioma...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Meneghini AC, Koenigkam-Santos M, Pereira MC, Tonidandel PR, Terra-Filho J, Cunha FQ, Menezes MB, Vianna EO Tags: Braz J Med Biol Res Source Type: research

Systemic injection of an H4 receptor agonist induces a decrease in CREB and pCREB levels in the cerebellar vermis and prefrontal cortex in mice.
oli R Abstract Studies have shown that an injection with the histamine H4 receptor agonist VUF-8430 modulates emotional memory processes. In the present study, the aim was to verify if intraperitoneal (ip) injection of VUF-8430 (500 ng/kg) in mice affects the synthesis of proteins required for memory consolidation processes by activating the phosphorylation of CREB (pCREB) in classical structures linked to emotional memory (prefrontal cortex, amygdala, and hippocampus) and the cerebellar vermis, a structure that has also been recently implicated in emotional memory. The results obtained using western blot analysis...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Fernandes CEM, Serafim KR, Gianlorenço ACL, Mattioli R Tags: Braz J Med Biol Res Source Type: research

Skp2 inhibitor SKPin C1 decreased viability and proliferation of multiple myeloma cells and induced apoptosis.
This study aimed to determine the effect of SKPin C1 on MM. Normal B lymphocytes, THP-1 cells, and MM U266 and RPMI 8226 cells were exposed to various dosages of SKPin C1 for 48 h. Cell proliferation was determined by MTT, EdU staining, and cell cycle assays. Western blot assays were performed to assess intracellular protein levels of Skp2, p27, and cleaved caspase-3. The amount of ubiquitin attached to p27 was determined using an immunoprecipitation assay. The viability of U266 and RPMI 8226 cells was significantly inhibited by 10 μM SKPin C1 and the inhibitory effect was enhanced with increasing doses of SKPin C1. In ...
Source: Braz J Med Biol Res - May 1, 2019 Category: Research Authors: Yang Y, Yan W, Liu Z, Wei M Tags: Braz J Med Biol Res Source Type: research

Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis.
In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to Bax ratio was also seen in myocytes treated with H2S after H2O2-induced stress. Our findings indicated a therapeutic potentia...
Source: Braz J Med Biol Res - April 18, 2019 Category: Research Authors: Zhang YE, Huang GQ, Wu B, Lin XD, Yang WZ, Ke ZY, Liu J Tags: Braz J Med Biol Res Source Type: research

Effect of lncRNA HULC knockdown on rat secreting pituitary adenoma GH3 cells.
In conclusion, HULC tumor-promoting roles in secreting pituitary adenoma might be via down-regulating miR-130b, up-regulating FOXM1, and activating PI3K/AKT/mTOR and JAK1/STAT3 pathways. PMID: 30994730 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - April 18, 2019 Category: Research Authors: Rui QH, Ma JB, Liao YF, Dai JH, Cai ZY Tags: Braz J Med Biol Res Source Type: research

Egocentric and allocentric spatial memory in healthy aging: performance on real-world tasks.
Abstract Although normal aging has been related to several cognitive difficulties, other processes have been studied less, such as spatial memory. Our aim was to compare egocentric and allocentric memory in an elderly population using ecological tasks. Twenty-eight cognitively unimpaired participants performed Egocentric and Allocentric Spatial Memory Tasks, as well as Spatial Span from CANTAB, Benton's Judge of Line Orientation test (JoLO), and Montreal Cognitive Assessment test (MoCA). The results revealed that younger participants showed better performance than older participants on both the Egocentric and Allo...
Source: Braz J Med Biol Res - April 18, 2019 Category: Research Authors: Fernandez-Baizan C, Diaz-Caceres E, Arias JL, Mendez M Tags: Braz J Med Biol Res Source Type: research

Altered peripheral lymphocyte subsets in untreated systemic lupus erythematosus patients with infections.
The objective of this study was to evaluate the distribution of lymphocyte subsets in untreated SLE patients with infections. This was a cross-sectional study. Data from January 2017 to May 2018 were collected. Flow cytometry was used to measure the peripheral lymphocyte subsets including CD3+T cells, CD4+T cells, CD8+T cells, CD19+B cells, CD3-CD16+CD56NK cells, and CD3+CD16+CD56NKT cells in 25 healthy controls and 52 treatment-naive SLE patients, among whom 13 were complicated with infections. Association between the lymphocyte subsets and infections was further analyzed. SLE patients with infections (n=13) showed a sign...
Source: Braz J Med Biol Res - April 18, 2019 Category: Research Authors: Lu Z, Li J, Ji J, Gu Z, Da Z Tags: Braz J Med Biol Res Source Type: research

Sepsis: evolving concepts and challenges.
unialti MKC Abstract Sepsis remains a major cause of morbidity and mortality worldwide, with increased burden in low- and middle-resource settings. The role of the inflammatory response in the pathogenesis of the syndrome has supported the modern concept of sepsis. Nevertheless, a definition of sepsis and the criteria for its recognition is a continuous process, which reflects the growing knowledge of its mechanisms and the success and failure of diagnostic and therapeutic interventions. Here we review the evolving concepts of sepsis, from the "systemic inflammatory response syndrome triggered by infection&qu...
Source: Braz J Med Biol Res - April 18, 2019 Category: Research Authors: Salomão R, Ferreira BL, Salomão MC, Santos SS, Azevedo LCP, Brunialti MKC Tags: Braz J Med Biol Res Source Type: research

Differential expression of microRNA-411 and 376c is associated with hypertension in pregnancy.
Abstract Preeclampsia is a major reason of morbidity and mortality in pregnant women and perinatal fetus. Hence, it is of prime importance that diagnostic markers are defined to predict chances of preeclampsia in pregnant women. It has been previously shown that microRNA (miRNA)-376c expression is decreased in the placenta of preeclampsia patients at term. Even though this decrease was not mimicked in the placenta at the pre-term stage, miR-376c expression was decreased in the plasma of these patients as early as the second trimester. Plasma and placenta specimens were obtained from pregnant women having unifetal ...
Source: Braz J Med Biol Res - April 8, 2019 Category: Research Authors: Yang HL, Zhang HZ, Meng FR, Han SY, Zhang M Tags: Braz J Med Biol Res Source Type: research