Hippocampal formation volume, its subregions, and its specific contributions to visuospatial memory tasks.
Abstract Visuospatial memory (VSM) is the ability to represent and manipulate visual and spatial information. This cognitive function depends on the functioning of the hippocampal formation (HF), located in the medial portion of the temporal cortex. The present study aimed to investigate whether there is an association between the volume of the HF and performance in VSM tests. High-resolution structural images (T1) and neuropsychological tests evaluating VSM were performed on 31 healthy individuals. A VSM index was created by grouping 5 variables from 5 tasks (4 from the CANTAB battery and 1 from the Rey-Osterriet...
Source: Braz J Med Biol Res - July 31, 2020 Category: Research Authors: Shavitt T, Johnson INS, Batistuzzo MC Tags: Braz J Med Biol Res Source Type: research

Past, present, and future of COVID-19: a review.
Abstract SARS-CoV-2 has recently emerged, becoming a global threat, affecting directly all human beings owing to its morbidity and mortality and indirectly, due to the enormous economic and psychological impact produced by social isolation, the most effective measure so far, but unsustainable for a long period. The scientific effort to understand and control SARS-CoV-2 transmission and clinical impact has been huge, and important achievements are highlighted in this review. Diagnosis is central and is the first step in recognizing and fighting any infectious agent. Instrumental to that is the quality of the data, ...
Source: Braz J Med Biol Res - July 31, 2020 Category: Research Authors: Romano CM, Chebabo A, Levi JE Tags: Braz J Med Biol Res Source Type: research

Clostridium difficile toxins or infection induce upregulation of adenosine receptors and IL-6 with early pro-inflammatory and late anti-inflammatory pattern.
en CA Abstract Clostridium difficile causes intestinal inflammation, which increases adenosine. We compared the expression of adenosine receptors (AR) subtypes A1, A2A, A2B, and A3 in HCT-8, IEC-6 cells, and isolated intestinal epithelial cells, challenged or not with Clostridium difficile toxin A and B (TcdA and TcdB) or infection (CDI). In HCT-8, TcdB induced an early A2BR expression at 6 h and a late A2AR expression at 6 and 24 h. In addition, both TcdA and TcdB increased IL-6 expression at all time-points (peak at 6 h) and PSB603, an A2BR antagonist, decreased IL-6 expression and production. In isolated cecum ...
Source: Braz J Med Biol Res - July 31, 2020 Category: Research Authors: Foschetti DA, Braga-Neto MB, Bolick D, Moore J, Alves LA, Martins CS, Bomfin LE, Santos A, Leitão R, Brito G, Warren CA Tags: Braz J Med Biol Res Source Type: research

Antiproliferative and photoprotective activities of the extracts and compounds from Calea fruticosa.
o AMD Abstract In this paper, we complement our previous study on the antiproliferative activity of Calea fruticosa (Asteraceae) by isolating the compounds apigenin-4',7-dimethyl ether (1), budlein A (2), quercetin (3), and cichoriin (4) from the plant's aerial parts. The antiproliferative activity of these compounds was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against human tumor cell lines. Compound 3 displayed moderate antiproliferative activity in three cell lines (HCT-116, PC-3, and SF-295, with cell growth inhibition values of 72.97, 74.55, and 68.94%) and hi...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Seregheti TMQ, Pinto APR, Gonçalves MDC, Antunes ADS, Almeida WADS, Machado RS, Silva JN, Ferreira PMP, Pessoa C, Santos VMRD, Nascimento AMD Tags: Braz J Med Biol Res Source Type: research

Fulminant type 1 diabetes mellitus in pregnancy.
Abstract Fulminant type 1 diabetes mellitus (FT1DM) has received clinical attention for its low incidence and poor prognosis. Currently, few cases of FT1DM are associated with pregnancy in clinical practice, but it poses a great threat to the life of mothers and infants. Here, we present two cases of FT1DM in pregnancy. In Case 1, the patient was a 26-year-old woman who was admitted to the hospital with reduced fetal movement. She was diagnosed with FT1DM and delivered a dead female fetus. Lispro and lantus were administered to control blood glucose, and lipoic acid for antioxidant therapy. In Case 2, the patient ...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Li CY, Li Y, You ZY, Liu YR, Wang YJ, Mu T, Zhao CZ, Yi ZM, Zhang YW Tags: Braz J Med Biol Res Source Type: research

Identification of potential molecular mechanisms and small molecule drugs in myocardial ischemia/reperfusion injury.
Abstract Myocardial ischemia/reperfusion (MI/R) injury is a complex phenomenon that causes severe damage to the myocardium. However, the potential molecular mechanisms of MI/R injury have not been fully clarified. We identified potential molecular mechanisms and therapeutic targets in MI/R injury through analysis of Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were found between MI/R injury and normal samples, and overlapping DEGs were found between GSE61592 and GSE67308. Gene Ontology (GO) and pathway analysis were performed for overlapping DEGs by Database for Annotation, Visuali...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Jiang T, Liu Y, Chen B, Si L Tags: Braz J Med Biol Res Source Type: research

An intensive education program for caregivers ameliorates anxiety, depression, and quality of life in patients with drug-resistant temporal lobe epilepsy and mesial temporal sclerosis who underwent cortico-amygdalohippocampectomy.
In conclusion, CIEP for caregivers contributed to the recovery of anxiety and depression as well as the improvement of QOL in patients with drug-resistant TLE-MTS who underwent CAH. PMID: 32696820 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Wang Y, Hou D, Wu X, Qiu L, Chen H, Xin J, Yan Z, Sun M Tags: Braz J Med Biol Res Source Type: research

CLEC3B protects H9c2 cardiomyocytes from apoptosis caused by hypoxia via the PI3K/Akt pathway.
Abstract Ischemic heart disease (IHD) is one of the leading causes of death worldwide. C-type lectin domain family 3 member B (CLEC3B) is a C-type lectin superfamily member and is reported to promote tissue remodeling. The serum levels of CLEC3B are downregulated in patients with cardiovascular disease. However, the molecular mechanisms of CLEC3B in IHD is not well-characterized. Therefore, we overexpressed CLEC3B and silenced CLEC3B in H9c2 rat cardiomyocytes for the first time. We then constructed a model of IHD in vitro through culturing H9c2 cardiomyocytes in serum-free medium under oxygen-deficit conditions. ...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Lv F, Wang Z, Huang Y, Si A, Chen Y Tags: Braz J Med Biol Res Source Type: research

MicroRNA-770-5p contributes to podocyte injury via targeting E2F3 in diabetic nephropathy.
Abstract Diabetic nephropathy (DN) has been identified as the major cause of end-stage renal disease (ESRD) in most developed countries. MicroRNA-770-5p depletion could repress high glucose (HG)-triggered apoptosis in podocytes, and downregulation of E2F transcription factor 3 (E2F3) could facilitate podocyte injury. Nevertheless, whether E2F3 is involved in miR-770-5p knockdown-mediated improvement of DN is still unclear. The expression levels of miR-770-5p and E2F3 were detected in HG-treated podocytes by RT-qPCR. The expression levels of E2F3, apoptosis-related proteins Bcl-2 related X protein (Bax), B-cell lym...
Source: Braz J Med Biol Res - July 23, 2020 Category: Research Authors: Guo J, Han J, Liu J, Wang S Tags: Braz J Med Biol Res Source Type: research

Estrogen attenuates TGF- β1-induced EMT in intrauterine adhesion by activating Wnt/β-catenin signaling pathway.
In this study, we aimed to investigate the effects of estrogen on epithelial-mesenchymal transition (EMT) in normal and fibrotic endometrium, and the role of estrogen and Wnt/β-catenin signaling in the formation of endometrial fibrosis. CCK-8 and immunofluorescence assay were performed to access the proliferation of different concentrations of estrogen on normal human endometrial epithelial cells (hEECs). qRT-PCR and western blot assay were utilized to explore the effect of estrogen on EMT in normal and fibrotic endometrium, and main components of Wnt/β-catenin signaling pathway in vitro. Hematoxylin and eosin an...
Source: Braz J Med Biol Res - July 9, 2020 Category: Research Authors: Cao J, Liu D, Zhao S, Yuan L, Huang Y, Ma J, Yang Z, Shi B, Wang L, Wei J Tags: Braz J Med Biol Res Source Type: research

Erratum for: "Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition" [Braz J Med Biol Res (2020) 53(4): e8882].
Erratum for: "Puerarin inhibits hepatocellular carcinoma invasion and metastasis through miR-21-mediated PTEN/AKT signaling to suppress the epithelial-mesenchymal transition" [Braz J Med Biol Res (2020) 53(4): e8882]. Braz J Med Biol Res. 2020;53(7):e8882erratum Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20198882]. PMID: 32555930 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - June 21, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

Development of a three-channel automatic climbing training system for rat rehabilitation after ischemic stroke.
Abstract This paper reports the development of a three-channel automatic speed-matching climbing training system that could train three rats at the same time for rehabilitation after an ischemic stroke. An infrared (IR) remote sensor was installed at the end of each channel to monitor the real-time position of a climbing rat. This research was carried out in five stages: i) system design; ii) hardware circuit; iii) running speed control; iv) functional testing; and v) verification using an animal model of cerebral stroke. The rehabilitated group significantly outperformed the middle cerebral artery occlusion (MCAo...
Source: Braz J Med Biol Res - June 21, 2020 Category: Research Authors: Chen CC, Chang CP Tags: Braz J Med Biol Res Source Type: research

Correlation between amyloid deposits affecting renal compartments and glomerular filtration rate during renal biopsy in a renal amyloidosis case series.
Abstract Amyloidosis comprises a group of disorders that accumulate modified autologous proteins in organs, mainly the kidneys. Few studies have addressed the amyloid compartmental distribution and associated clinical outcomes. The aim of this study was to present a case series of renal amyloidosis correlating histopathological data with glomerular filtration rate (GFR) during kidney biopsy. We studied 53 cases reviewed by nephropathologists from 2000 to 2018 in a single kidney biopsy center in Brazil. GFR was estimated using the CKD-EPI formula. Cases were divided into Group A ≥60 and Group B
Source: Braz J Med Biol Res - May 20, 2020 Category: Research Authors: Fonseca EO, Caldas MLR, Soares Filho PJ, Almeida JR Tags: Braz J Med Biol Res Source Type: research

Animal models of human atherosclerosis: current progress.
Abstract Atherosclerosis retains the leading position among the causes of global morbidity and mortality worldwide, especially in the industrialized countries. Despite the continuing efforts to investigate disease pathogenesis and find the potential points of effective therapeutic intervention, our understanding of atherosclerosis mechanisms remains limited. This is partly due to the multifactorial nature of the disease pathogenesis, when several factors so different as altered lipid metabolism, increased oxidative stress, and chronic inflammation act together leading to the formation and progression of atheroscle...
Source: Braz J Med Biol Res - May 20, 2020 Category: Research Authors: Poznyak AV, Silaeva YY, Orekhov AN, Deykin AV Tags: Braz J Med Biol Res Source Type: research

Voxel-wise meta-analysis of structural changes in gray matter of Parkinson's disease patients with mild cognitive impairment.
Abstract Evidence from previous voxel-based morphometry (VBM) studies indicates that widespread brain regions are involved in Parkinson's disease with mild cognitive impairment (PD-MCI). However, the spatial localization reported for gray matter (GM) abnormalities is heterogeneous. The aim of the present study was to quantitatively integrate studies on GM abnormalities observed in PD-MCI in order to determine whether a pattern exists. Eligible whole-brain VBM studies were identified by a systematic search of articles in PubMed and EMBASE databases spanning from 1995 to January 1, 2019. A meta-analysis was performe...
Source: Braz J Med Biol Res - May 20, 2020 Category: Research Authors: Qin B, Yang MX, Gao W, Zhang JD, Zhao LB, Qin HX, Chen H Tags: Braz J Med Biol Res Source Type: research

Notoginsenoside R1 upregulates miR-221-3p expression to alleviate ox-LDL-induced apoptosis, inflammation, and oxidative stress by inhibiting the TLR4/NF- κB pathway in HUVECs.
This study aimed to explore the mechanism of NGR1 for treating AS through miRNAs. Flow cytometry was used to detect the apoptosis rate. The levels of inflammatory cytokines interleukin (IL)-6 and IL-1β were detected using ELISA. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels were measured using corresponding assay kits. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was performed to detect miR-221-3p expression. Dual-luciferase reporter and RNA immunoprecipitation assays were carried out to examine the relationship between miR-221-3p and toll-like receptors 4 (TLR4). Also, western blo...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Zhu L, Gong X, Gong J, Xuan Y, Fu T, Ni S, Xu L, Ji N Tags: Braz J Med Biol Res Source Type: research

High levels of chemerin associated with variants in the NOS3 and APOB genes in rural populations of Ouro Preto, Minas Gerais, Brazil.
GLL Abstract Chemerin is an adipokine that has been associated with components of metabolic syndrome. It has been described to affect adipocyte metabolism and inflammatory responses in adipose tissue, as well as the systemic metabolism of lipids and glucose. Few epidemiological studies have evaluated classical and genetics cardiovascular risk factors (CVRFs) in the mixed adult rural population in Brazil. Therefore, the present study explored possible associations between CVRFs and chemerin. This cross-sectional study included 508 adults from the rural localities of Lavras Novas, Chapada, and Santo Antônio d...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Batista AP, Barbosa KF, Masioli CZ, Queiroz EM, Marinho CC, Cândido APC, Machado-Coelho GLL Tags: Braz J Med Biol Res Source Type: research

Treatment with β-elemene combined with paclitaxel inhibits growth, migration, and invasion and induces apoptosis of ovarian cancer cells by activation of STAT-NF-κB pathway.
In conclusion, the data suggested that β-elemene+paclitaxel can inhibit ovarian cancer growth via down-regulation of the STAT3-NF-κB signaling pathway, which may be a potential therapeutic strategy for ovarian cancer therapy. PMID: 32401925 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Xiaomeng F, Lei L, Jinghong A, Juan J, Qi Y, Dandan Y Tags: Braz J Med Biol Res Source Type: research

Aging and low-intensity exercise change oxidative biomarkers in brain regions and radiographic measures of femur of Wistar rats.
Abstract We investigated changes in oxidative biomarkers in brain regions such as brainstem, cerebellum, and cerebral cortex of 3-, 6-, 18-, 24-, and 30-month-old rats. We also assessed the effects of low-intensity exercise on these biomarkers in these regions of 6-, 18-, and 24-month-old rats that started exercise on a treadmill at 3, 15, and 21 months of age, respectively. Radiographic images of the femur were taken for all rats. A total of 25 rats (age: twelve 6-, ten 18-, ten 24-, and three 30-month-old rats) were used. Lipid hydroperoxide levels increased in cerebellum at 18 months. Total antioxidant activity...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Silveira EMS, Santos MCQ, da Silva TCB, Silva FBO, Machado CV, Elias L, Kolberg A, Kroth A, Partata WA Tags: Braz J Med Biol Res Source Type: research

Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund's adjuvant-induced rheumatoid arthritis via inhibition of NF- κB.
This study was taken to identify the effect of rhoifolin on complete Freund's adjuvant (CFA)-induced arthritis in the rat model. Treatment with rhoifolin (10 and 20 mg/kg) showed a significant improvement in the overall health parameters such as paw edema and weight loss. This improvement in morphological parameters corroborated the findings with gross morphological changes observed in the histopathological analysis. Rhoifolin treatment also caused a significant decrease in oxidative stress, evident from changes in intracellular levels of glutathione, glutathione peroxidase, malondialdehyde, and superoxide dismutase in the...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Peng S, Hu C, Liu X, Lei L, He G, Xiong C, Wu W Tags: Braz J Med Biol Res Source Type: research

Effect of ethanol extracts of Antrodia cinnamomea on head and neck squamous cell carcinoma cell line.
Abstract Head and neck squamous cell carcinoma (HNSCC) is one of the most common malignant tumors. Ethanol extract of Antrodia cinnamomea (EEA) has been widely studied for its health benefits including anticancer effects. The purpose of this study was to assess the effects of EEA on HNSCC. Cell proliferation, transwell, and wound healing assays were performed. The impact of EEA on tumor growth was investigated using a xenograft model. Expressions of migration-related proteins (MMP-2, MMP-9, TIMP-1, and TIMP-2) and apoptosis-related proteins (cleaved caspase-9 and cleaved PARP) were determined using western blot an...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Liu L, Wang C Tags: Braz J Med Biol Res Source Type: research

A Brazilian regional basic diet-induced chronic malnutrition drives liver inflammation with higher ApoA-I activity in C57BL6J mice.
This study aimed to investigate whether RBD leads to liver inflammatory changes and altered reverse cholesterol metabolism in C57BL6/J mice compared to the control group, receiving a standard chow diet. To evaluate liver inflammation, ionized calcium-binding adapter protein-1 (IBA-1) positive cell counting, interleukin (IL)-1β immunohistochemistry, and tumor necrosis factor (TNF)-α and IL-10 transcription levels were analyzed. In addition, we assessed reverse cholesterol transport by measuring liver apolipoprotein (Apo)E, ApoA-I, and lecithin-cholesterol acyltransferase (LCAT) by RT-PCR. Furthermore, serum alani...
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Santos MJS, Canuto KM, de Aquino CC, Martins CS, Brito GAC, Pessoa TMRP, Bertolini LR, de Sá Carneiro I, Pinto DV, Nascimento JCR, da Silva BB, Valença JT, Guedes MIF, Owen JS, Oriá RB Tags: Braz J Med Biol Res Source Type: research

Evaluation of factors for predicting risk of uveitis recurrence in Behcet's disease patients.
In conclusion, uveitis occurrence times within 3 months, oral ulcers, TG, LDL, and SAA might be potential predictive factors for UR risk in BD patients, which can help in prevention and management of the disease. PMID: 32401930 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - May 14, 2020 Category: Research Authors: Cai J, Qi L, Chen Y, Zou J, Shen Y, Luo D, Bao H, Ye J, Ma H, Guan JL Tags: Braz J Med Biol Res Source Type: research

Expression of CHPF modulates cell proliferation and invasion in lung cancer.
In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony formation. Wound healing and transwell invasion assays revealed that CHPF could positively regulate the migration of lung cancer cells. The tumorigenic role of CHPF was also validated in nude mouse xenograft models. Affymetrix gene chip analysis indicated that C...
Source: Braz J Med Biol Res - April 30, 2020 Category: Research Authors: Cao C, Liu Y, Wang Q, Zhao J, Shi M, Zheng J Tags: Braz J Med Biol Res Source Type: research

Effect of Camellia sinensis teas on left ventricular hypertrophy and insulin resistance in dyslipidemic mice.
In conclusion, the three Camellia sinensis L. teas used to control genetic dyslipidemia associated with a high-fat diet can be used as an auxiliary treatment associated with the control of lipid intake, thus promoting cardiac protection against hyperlipidemia. PMID: 32348424 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - April 30, 2020 Category: Research Authors: Ferreira MCL, Lima LN, Cota LHT, Costa MB, Orsi PME, Espíndola RP, Albanez AV, Rosa BB, Carvalho MGS, Garcia JAD Tags: Braz J Med Biol Res Source Type: research

miR-22 and cerebral microbleeds in brainstem and deep area are associated with depression one month after ischemic stroke.
In this study, we aimed to explore the relationship among miR-22, deep cerebral microbleeds (CMBs), and post-stroke depression (PSD) 1 month after ischemic stroke. We consecutively recruited 257 patients with first-ever and recurrent acute cerebral infarction and performed PSD diagnosis in accordance with the Diagnostic and Statistical Manual IV criteria for depression. Clinical information, assessments of stroke severity, and imaging data were recorded on admission. We further detected plasma miR-22 using quantitative PCR and analyzed the relationship among miR-22, clinical data, and PSD using SPSS 23.0 software. Logistic...
Source: Braz J Med Biol Res - April 30, 2020 Category: Research Authors: Hu J, Zhou W, Zhou Z, Yang Q, Xu J, Dong W Tags: Braz J Med Biol Res Source Type: research

Evaluation of the cytotoxic and genotoxic effects by melamine and cyanuric acid co-exposure in human embryonic kidney 293 cells.
Abstract The melamine and cyanuric acid (CA) complex has been suggested to cause the toxic effects observed in melamine-contaminated food or milk. However, the cytotoxic and genotoxic effects of co-exposure to melamine and CA are not fully clear. Therefore, the cytotoxic effects of melamine and CA were first examined by co-exposure in human kidney 293 cells using the MTT assay. During a 24-h period for the three concentrations tested (0.5, 1, and 5 mg/mL), neither melamine nor CA alone showed significant toxic effects on 293 cells at 0.5 mg/mL, while higher concentrations led to decreased in cell viability. Howeve...
Source: Braz J Med Biol Res - April 30, 2020 Category: Research Authors: Xu X, Lu J, Sheng H, Zhang L, Gan T, Zhang J, Xu Y, Zhu X, Yang J Tags: Braz J Med Biol Res Source Type: research

Indomethacin attenuates mechanical allodynia during the organization but not the maintenance of the peripheral neuropathic pain induced by nervus ischiadicus chronic constriction injury.
Abstract The neurochemical mechanisms underlying neuropathic pain (NP) are related to peripheral and central sensitization caused by the release of inflammatory mediators in the peripheral damaged tissue and ectopic discharges from the injured nerve, leading to a hyperexcitable state of spinal dorsal horn neurons. The aim of this work was to clarify the role played by cyclooxygenase (COX) in the lesioned peripheral nerve in the development and maintenance of NP by evaluating at which moment the non-steroidal anti-inflammatory drug indomethacin, a non-selective COX inhibitor, attenuated mechanical allodynia after p...
Source: Braz J Med Biol Res - April 30, 2020 Category: Research Authors: Medeiros P, Dos Santos IR, Medeiros AC, da Silva JA, Ferreira SH, de Freitas RL, Coimbra NC Tags: Braz J Med Biol Res Source Type: research

Enhanced anti-tumor efficacy of 5-aminolevulinic acid-gold nanoparticles-mediated photodynamic therapy in cutaneous squamous cell carcinoma cells.
In conclusion, our results suggested that GNPs conjugated to 5-ALA significantly enhanced the anti-tumor efficacy of PDT in A431 cells, which may represent a better strategy to improve the outcomes of patients with cutaneous squamous cell carcinoma. PMID: 32348428 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - April 30, 2020 Category: Research Authors: Chi YF, Qin JJ, Li Z, Ge Q, Zeng WH Tags: Braz J Med Biol Res Source Type: research

miR-378a-5p and miR-630 induce lens epithelial cell apoptosis in cataract via suppression of E2F3.
Abstract Cataract, an eye disease that threatens the health of millions of people, brings about severe economic burden for patients and society. MicroRNA (miR)-378a-5p and miR-630 were recognized as essential regulators in multiple cancers. However, the exact functions of miR-378a-5p and miR-630 in cataract are still unclear. The expression of miR-378a-5p, miR-630, and E2F transcription factor 3 (E2F3) in tissues and cells was measured by quantitative real-time polymerase chain reaction. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay was used to evaluate cell viability. Flow cytometry ...
Source: Braz J Med Biol Res - April 30, 2020 Category: Research Authors: Gao W, Zhou X, Lin R Tags: Braz J Med Biol Res Source Type: research

Impact of cigarette smoke and aerobic physical training on histological and molecular markers of prostate health in rats.
Abstract Recent evidence suggests that aerobic physical training may attenuate the deleterious effects of cancer risk factors, including smoking. We investigated the effects of cigarette smoke inhalation and aerobic physical training on the expression of steroid receptors and inflammatory and apoptotic proteins in the prostate. Forty male Wistar rats were distributed in four groups: control (CO), exercise (EXE), cigarette smoke exposure (CS), and cigarette smoke exposure with exercise (CS+EXE). For eight weeks, animals were repeatedly exposed to cigarette smoke for 30 min or performed aerobic physical training eit...
Source: Braz J Med Biol Res - April 23, 2020 Category: Research Authors: Veras ASC, Baptista DB, Dos Santos NJ, Thorpe HHA, Seraphim PM, Florido Neto AR, Teixeira GR Tags: Braz J Med Biol Res Source Type: research

Alanyl-glutamine protects the intestinal barrier function in trained rats against the impact of acute exhaustive exercise.
Abstract Strenuous exercise triggers deleterious effects on the intestinal epithelium, but their mechanisms are still uncertain. Here, we investigated whether a prolonged training and an additional exhaustive training protocol alter intestinal permeability and the putative effect of alanyl-glutamine (AG) pretreatment in this condition. Rats were allocated into 5 different groups: 1) sedentary; 2 and 3) trained (50 min per day, 5 days per week for 12 weeks) with or without 6 weeks oral (1.5 g/kg) AG supplementation; 4 and 5) trained and subjected to an additional exhaustive test protocol with or without oral AG sup...
Source: Braz J Med Biol Res - April 23, 2020 Category: Research Authors: Freitas AKL, Silva MTB, Silva CMS, Prata MMG, Rodrigues FAP, Siqueira RJB, Lima AAM, Santos AA, Havt A Tags: Braz J Med Biol Res Source Type: research

MALAT1 is involved in type I IFNs-mediated systemic lupus erythematosus by up-regulating OAS2, OAS3, and OASL.
This study suggested the involvement of MALAT1 in type I IFNs-mediated SLE by up-regulating OAS2, OAS3, and OASL. PMID: 32321151 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - April 23, 2020 Category: Research Authors: Gao F, Tan Y, Luo H Tags: Braz J Med Biol Res Source Type: research

Tissue fusion technology versus suture and staple in porcine bowel anastomosis: an in vivo study.
Abstract The aim of this study was to make a comparison between the tissue fusion technique and conventional methods for sealing bowel anastomosis. Eighteen female domestic pigs (Suidae, Sus) were used in our study. Tissue-fused anastomoses (LigaSure groups) were made in 13 animals (5 anastomoses per animal), which were subdivided into 4 groups according to different manufacturing settings: "LigaSure-L-1" and "LigaSure-L-2", with low energy output level with 1 or 2 device-activated tissue sealing times, and "LigaSure-M" and "LigaSure-H", with medium or high energy output lev...
Source: Braz J Med Biol Res - April 23, 2020 Category: Research Authors: Pan H, Leung KKC, Ng EKW Tags: Braz J Med Biol Res Source Type: research

MicroRNA-4290 suppresses PDK1-mediated glycolysis to enhance the sensitivity of gastric cancer cell to cisplatin.
In conclusion, this study revealed that miR-4290 suppressed PDK1-mediated glycolysis to enhance the sensitivity of GC cells to cisplatin. PMID: 32321153 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - April 23, 2020 Category: Research Authors: Qian Y, Wu X, Wang H, Hou G, Han X, Song W Tags: Braz J Med Biol Res Source Type: research

Chronic aerobic exercise associated to low-dose L-NAME improves contractility without changing calcium handling in rat cardiomyocytes.
In conclusion, the association of chronic aerobic exercise and low-dose L-NAME prevented cardiac pathological remodeling and induced cardiomyocyte contractile function improvement; however, it did not alter myocyte affinity and sensitivity to intracellular Ca2+ handling. PMID: 32159612 [PubMed - in process] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - March 13, 2020 Category: Research Authors: Luchi TC, Coelho PM, Cordeiro JP, Assis ALEM, Nogueira BV, Marques VB, Dos Santos L, Lima-Leopoldo AP, Lunz W, Leopoldo AS Tags: Braz J Med Biol Res Source Type: research

Kidney health for everyone everywhere - from prevention to detection and equitable access to care.
This article focuses on outlining and analyzing measures that can be implemented in every country to promote and advance CKD prevention. Primary prevention of kidney disease should focus on the modification of risk factors and addressing structural abnormalities of the kidney and urinary tracts, as well as exposure to environmental risk factors and nephrotoxins. In persons with pre-existing kidney disease, secondary prevention, including blood pressure optimization and glycemic control, should be the main goal of education and clinical interventions. In patients with advanced CKD, management of co-morbidities such as uremi...
Source: Braz J Med Biol Res - March 13, 2020 Category: Research Authors: Li PK, Garcia-Garcia G, Lui SF, Andreoli S, Fung WW, Hradsky A, Kumaraswami L, Liakopoulos V, Rakhimova Z, Saadi G, Strani L, Ulasi I, Kalantar-Zadeh K, World Kidney Day 2020 Steering Committee Tags: Braz J Med Biol Res Source Type: research

Effects of NO/cGMP inhibitors in a rat model of anaphylactoid shock.
This study aimed to investigate the effects of NO/cGMP pathway inhibitors methylene blue (MB), Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), and indigo carmine (IC) in shock induced by compound 48/80 (C48/80) in rats. The effect was assessed by invasive blood pressure measurement. Shock was initiated by C48/80 intravenous bolus injection 5 min before (prophylactic) or after (treatment) the administration of the inhibitors MB (3 mg/kg), L-NAME (1 mg/kg), and IC (3 mg/kg). Of the groups that received drugs as prophylaxis for shock, only the IC group did not present the final systolic blood pressure (SBP) bet...
Source: Braz J Med Biol Res - March 5, 2020 Category: Research Authors: Albuquerque AAS, Ferreira LG, Carvalho MTM, Capellini VK, Evora PRB, Celotto AC Tags: Braz J Med Biol Res Source Type: research

Up-regulated miR-106b inhibits ox-LDL-induced endothelial cell apoptosis in atherosclerosis.
Abstract This research aimed to explore the molecular mechanism of microRNA (miR)-106b in cell apoptosis of atherosclerosis (AS). Human aortic endothelial cells (HAECs) were divided into control group, oxidized-low-density lipoproteins (ox-LDL) group, miR-106b NC+ox-LDL group, miR-106b mimics+ox-LDL group, miR-106b mimics+PTEN+ox-LDL group, and miR-106b mimics+empty+ox-LDL group. Real-time fluorescence quantitative polymerase chain reaction, cholecystokinin, TdT-mediated biotinylated nick end-labeling assay, luciferase reporter gene assay, and flow cytometry analysis were performed to determine the morphology, pro...
Source: Braz J Med Biol Res - March 5, 2020 Category: Research Authors: Zhang Y, Wang L, Xu J, Kong X, Zou L Tags: Braz J Med Biol Res Source Type: research

Repercussions of adjuvant-induced arthritis on body composition, soleus muscle, and heart muscle of rats.
In conclusion, AIA-induced changes in body composition as well as in the soleus muscle fibers and vasculature have early onset but are more evident by the 15th day after induction. Moreover, the heart may be a target organ of AIA, although less sensitive than skeletal muscles. PMID: 32130291 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - March 5, 2020 Category: Research Authors: Pita LM, Spadella MA, Montenote MC, Oliveira PB, Chies AB Tags: Braz J Med Biol Res Source Type: research

The APOB rs693 polymorphism impacts the lipid profile of Brazilian older adults.
The objective of this study was to verify the association of the classic APOB Xba I polymorphism (rs693) with atherosclerotic risk factors in a segment of the Brazilian elderly population considering their usual dietary intake. Clinical and biochemical characteristics as well as total caloric and fat intake data were determined from 644 elderly individuals. Polymorphism analysis was performed by conventional polymerase chain reaction followed by enzyme restriction. Statistical analyses compared measures and proportions according to different APOB genotypic combinations. Statistically significant association was found betwe...
Source: Braz J Med Biol Res - March 5, 2020 Category: Research Authors: Alves ES, Henriques AD, Tonet-Furioso AC, Paula RS, Gomes LO, Moraes CF, Nóbrega OT Tags: Braz J Med Biol Res Source Type: research

Methylophiopogonanone A, an Ophiopogon homoisoflavonoid, alleviates high-fat diet-induced hyperlipidemia: assessment of its potential mechanism.
This study was performed to investigate a potential hypolipidemic effect of MO-A in hyperlipidemia rats, as well as its underlying mechanism of action. A rat model of hyperlipidemia was induced by a high-fat diet (HFD). Animals were randomly divided into three groups (n=8/group): normal control group (NC), HFD group, and HFD+MO-A (10 mg·kg-1·d-1) treatment group. The effects of MO-A on serum lipids, body weight, activity of lipoprotein metabolism enzyme, and gene expression of lipid metabolism were evaluated in HFD-induced rats. In HFD-induced rats, pretreatment with MO-A decreased the body weight gain and re...
Source: Braz J Med Biol Res - March 5, 2020 Category: Research Authors: Li Z, Wu YY, Yu BX Tags: Braz J Med Biol Res Source Type: research

Erratum for: miR-26a-5p protects against myocardial ischemia/reperfusion injury by regulating the PTEN/PI3K/AKT signaling pathway.
Authors: Abstract [This corrects the article doi: 10.1590/1414-431X20199106]. PMID: 32130293 [PubMed - as supplied by publisher] (Source: Braz J Med Biol Res)
Source: Braz J Med Biol Res - March 2, 2020 Category: Research Tags: Braz J Med Biol Res Source Type: research

p19Arf sensitizes B16 melanoma cells to interferon- β delivered via mesenchymal stem cells in vitro.
p19Arf sensitizes B16 melanoma cells to interferon-β delivered via mesenchymal stem cells in vitro. Braz J Med Biol Res. 2020;53(3):e8876 Authors: Da-Costa RC, Vieira IL, Hunger A, Tamura RE, Strauss BE Abstract The immune stimulatory and anti-neoplastic functions of type I interferon have long been applied for the treatment of melanoma. However, the systemic application of high levels of this recombinant protein is often met with toxicity. An approach that provides localized, yet transient, production of type I interferon may overcome this limitation. We propose that the use of mesenchymal stem ...
Source: Braz J Med Biol Res - February 22, 2020 Category: Research Authors: Da-Costa RC, Vieira IL, Hunger A, Tamura RE, Strauss BE Tags: Braz J Med Biol Res Source Type: research

High frequency of Y chromosome microdeletions in male infertility patients with 45,X/46,XY mosaicism.
Abstract The mosaic 45,X/46,XY karyotype is a common sex chromosomal abnormality in infertile men. Males with this mosaic karyotype can benefit from assisted reproductive therapies, but the transmitted abnormalities contain 45,X aneuploidy as well as Y chromosome microdeletions. The aim of this study was to investigate the clinical and genetic characteristics of infertile men diagnosed with 45,X/46,XY mosaicism in China. Of the 734 infertile men found to carry chromosomal abnormalities, 14 patients were carriers of 45,X/46,XY mosaicism or its variants, giving a prevalence of 0.27% (14/5269) and accounting for 1.91...
Source: Braz J Med Biol Res - February 22, 2020 Category: Research Authors: Li L, Zhang H, Yang Y, Zhang H, Wang R, Jiang Y, Liu R Tags: Braz J Med Biol Res Source Type: research

A high-carbohydrate diet induces greater inflammation than a high-fat diet in mouse skeletal muscle.
Abstract We previously reported that both the high-carbohydrate diet (HCD) and high-fat diet (HFD) given for two months promote lipid deposition and inflammation in the liver and brain of mice. The results obtained indicate a tissue-specific response to both diets. Herein, we compared the effects of HCD and HFD on fatty acid (FA) composition and inflammation in the gastrocnemius muscle. Male Swiss mice were fed with HCD or HFD for 1 or 2 months. Saturated FA (SFA), monounsaturated FA (MUFA), n-3 polyunsaturated FA (n-3 PUFA), and n-6 PUFA were quantified. The activities of stearoyl-CoA desaturase 1 (SCD-1), &Delta...
Source: Braz J Med Biol Res - February 22, 2020 Category: Research Authors: Antunes MM, Godoy G, de Almeida-Souza CB, da Rocha BA, da Silva-Santi LG, Masi LN, Carbonera F, Visentainer JV, Curi R, Bazotte RB Tags: Braz J Med Biol Res Source Type: research

Sexual function after total laparoscopic hysterectomy or transabdominal hysterectomy for benign uterine disorders: a retrospective cohort.
The objective of this study was to evaluate changes in sexual function after total laparoscopic hysterectomy (TLH) or transabdominal hysterectomy (TAH). This retrospective cohort study included patients with benign uterine tumors that were divided into TLH group and TAH group based on the hysterectomy technique used. Baseline, intraoperative, and postoperative characteristics were compared between groups. Postoperative sexual function was assessed using the Brief Index of Sexual Functioning for Women. The TLH and TAH groups contained 119 patients (age, 51.5±6.1 years) and 126 patients (age, 50.0±4.7 years), r...
Source: Braz J Med Biol Res - February 22, 2020 Category: Research Authors: Wang Y, Ying X Tags: Braz J Med Biol Res Source Type: research

Effects of high- and moderate-intensity exercise on central hemodynamic and oxygen uptake recovery kinetics in CHF-COPD overlap.
Abstract The oxygen uptake (V˙O2) kinetics during onset of and recovery from exercise have been shown to provide valuable parameters regarding functional capacity of both chronic obstructive pulmonary disease (COPD) and chronic heart failure (CHF) patients. To investigate the influence of comorbidity of COPD in patients with CHF with reduced ejection fraction on recovery from submaximal exercise, 9 CHF-COPD male patients and 10 age-, gender-, and left ventricle ejection fraction (LVEF)-matched CHF patients underwent constant-load exercise tests (CLET) at moderate and high loads. The V˙O2, heart rate (HR), and ca...
Source: Braz J Med Biol Res - February 22, 2020 Category: Research Authors: Mazzuco A, Souza AS, Medeiros WM, Sperandio PA, Alencar MCN, Arbex FF, Neder JA, Borghi-Silva A Tags: Braz J Med Biol Res Source Type: research

SULF2 promotes tumorigenesis and inhibits apoptosis of cervical cancer cells through the ERK/AKT signaling pathway.
The objective of this study was to explore the role of the SULF2-mediated ERK/AKT signaling pathway in cervical cancer. SULF2 expression was detected in tumor tissues and tumor-adjacent normal tissues from cervical cancer patients. HeLa cells were divided into six groups: control group, NC group, SULF2 siRNA group, SULF2 group, SULF2 + LY294002 group, and SULF2 + U0125 group. In each group, HeLa cells received the corresponding treatment, followed by measurement of the cellular biological characteristics and expression of the ERK/AKT signaling pathway. We also confirmed the effect of SULF2 in vivo using a xenograft model i...
Source: Braz J Med Biol Res - February 13, 2020 Category: Research Authors: Jiang T, Chen ZH, Chen Z, Tan D Tags: Braz J Med Biol Res Source Type: research

Tai Chi versus routine exercise in patients with early- or mild-stage Parkinson's disease: a retrospective cohort analysis.
The objective of the study was to compare the effect of routine exercises and Tai Chi on physical and clinical performance in elderly people suffering from Parkinson's disease. Data from interviews, physical and clinical performance, and levodopa consumption of 500 patients with confirmed Parkinson's disease (severity level I to III) were collected and analyzed. Participants who received 80 min/day Tai Chi 3 times/week for 2 months were included in the Tai Chi (TC) group (n=250) and those who received 90 min/day routine exercise 3 times/week for 2 months were included in routine exercise (RE) group (n=250). Timed up-and-go...
Source: Braz J Med Biol Res - February 13, 2020 Category: Research Authors: Li Q, Liu J, Dai F, Dai F Tags: Braz J Med Biol Res Source Type: research