Decrease in serum levels of adiponectin and increase in 8-OHdG: A culprit for cognitive impairment in the elderly with type 2 diabetes.
CONCLUSION: Serum levels of adiponectin and 8-OHdG could act as specific and sensitive bio-markers for the early diagnosis and treatment of cognitive impairment in elderly T2DM patients. Serum levels of adiponectin and 8-OHdG have a close relation to the neurological cognitive outcome of the elderly T2DM patients. PMID: 31424368 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - August 19, 2019 Category: Molecular Biology Authors: Huang TF, Tang ZP, Wang S, Hu MW, Zhan L, Yi Y, He YL, Cai ZY Tags: Curr Mol Med Source Type: research

Molecular and Clinical Investigations on Portuguese Patients with Multiple acyl-CoA Dehydrogenase Deficiency.
CONCLUSION: Advanced molecular diagnosis, structural analysis and clinical correlations reveal that MADD patients harboring a severe prognosis mutation in one allele can actually revert to a milder phenotype by complementation with a milder mutation in the other allele. However, such patients are nevertheless in a precarious metabolic balance which can revert to severe fatal outcomes during catabolic stress or secondary pathology, thus requiring strict clinical follow-up. PMID: 31418342 [PubMed - in process] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - August 18, 2019 Category: Molecular Biology Authors: Henriques BJ, Lucas TG, Martins E, Gaspar A, Bandeira A, Nogueira C, Brandão O, Rocha H, Vilarinho L, Gomes CM Tags: Curr Mol Med Source Type: research

Characteristics of Natural Killer Cell Interaction with Trophoblast Cells During Pregnancy.
CONCLUSION: Secretory products of the placenta increase receptor expression to ensure trophoblast adhesion to the matrix and increase the already sufficiently strong adhesion and migration potential of NK cells. PMID: 31393246 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - August 7, 2019 Category: Molecular Biology Authors: Bazhenov DO, Khokhlova EV, Viazmina LP, Furaeva KN, Mikhailova VA, Kostin NA, Selkov SA, Sokolov DI Tags: Curr Mol Med Source Type: research

Autosomal recessive non-syndromic keratoconus: Homozygous frameshift variant in the candidate novel gene GALNT14.
In this study, we used whole exome sequencing to identify the genetic cause of KC in two sibs in a consanguineous family. The Homozygous frameshift variant NM_001253826.1:c.60delC;p.Leu21Cysfs*6 was identified in the gene N-acetylgalactosaminyltransferase 14 (GALNT14). The variant does not exist in all public databases neither in our internal exome database. Moreover, no database harbours homozygous loss of function variants in the candidate gene. GALNT14 catalyses the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on target p...
Source: Current Molecular Medicine - July 29, 2019 Category: Molecular Biology Authors: Froukh T, Hawwari A Tags: Curr Mol Med Source Type: research

Inhibition of Histone Deacetylase 6 Protects Hippocampal Cells Against Mitochondria-mediated Apoptosis in a Model of Severe Oxygen-glucose Deprivation.
CONCLUSIONS: HDAC 6 inhibition, using Tub-A, protects against OGD-induced injury in HT22 cells by modulating Akt/GSK3β signaling and inhibiting mitochondria-mediated apoptosis. PMID: 31339071 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - July 23, 2019 Category: Molecular Biology Authors: Chang P, Tian Y, Williams AM, Bhatti UF, Liu B, Li Y, Alam HB Tags: Curr Mol Med Source Type: research

Small regulatory molecules acting big in cancer: Potential Role of mito-miRs in cancer.
Abstract MicroRNAs [miRNAs] are short, non-coding, single stranded molecules regulating gene expression of the target molecule at the posttranscriptional level by either degrading mRNA or by inhibiting translation. Previously, miRNAs have been reported to be present inside the mitochondria and termed as mito-miRs. Origin of these mito-miRs may either be from mitochondrial genome or import from nucleus. The second class of mito-miRs makes it important to unravel the involvement of miRNAs in crosstalk between nucleus and mitochondria. Since, miRNAs are involved in various physiological processes, thus their deregula...
Source: Current Molecular Medicine - July 23, 2019 Category: Molecular Biology Authors: Sharma P, Kumar B, Dogra N, Singh S Tags: Curr Mol Med Source Type: research

Exosome: An Emerging Source of Biomarkers for Human Diseases.
Abstract Exosomes are 30-120nm long endocytic membrane-derived vesicles, which are secreted by various types of cells and stably present in body fluids, such as plasma, urine, saliva and breast milk. Exosomes participate in intercellular communication. Recently accumulative studies have suggested that exosomes may serve as novel biomarkers for disease diagnosis and prognosis. Herein, we reviewed the biological features of exosomes, technologies for exosome isolation and identification, as well as progress in exosomal biomarker identification, highlighting the relevance of exosome to human diseases and significance...
Source: Current Molecular Medicine - July 12, 2019 Category: Molecular Biology Authors: Xu L, Wu LF, Deng FY Tags: Curr Mol Med Source Type: research

Inhibition of Experimental Age-Related Macular Degeneration by ZQMT in Mice.
CONCLUSION: In summary, our study supports the protective roles of a traditional Chinese medicine ZQMT in AMD. PMID: 31288713 [PubMed - in process] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - July 12, 2019 Category: Molecular Biology Authors: Yang L, Meng H, Luo D, Deng T, Miao L, Zou B, Ge X, Hu X, Liu Y, Li X, Deng X, Guo S, Liang J, Chen T, Wen X, Li JJ, Wei L, Jin M Tags: Curr Mol Med Source Type: research

Polymorphisms of the Highly Expressed IL-6 Gene in the Papillary Thyroid Cancer Susceptibility Among Chinese.
CONCLUSION: Our study indicates that IL-6 variants may be a risk factor involved in the pathogenesis and development of PTC. PMID: 31288714 [PubMed - in process] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - July 12, 2019 Category: Molecular Biology Authors: Li H, Dai H, Li H, Li B, Shao Y Tags: Curr Mol Med Source Type: research

Stem cells as a resource for treatment infertility-related diseases.
Abstract Infertility has become a common problem in much of the world. The problem is so serious that is now considered a new disease by the World Health Organization (WHO). The WHO defines infertility as a couple's inability to achieve pregnancy despite having frequent unprotected sexual intercourse for at least a year. So defined, infertility affects 8%-12% of couples of reproductive age, worldwide. There are many ways to treat infertility, including medication, intrauterine insemination, and in vitro fertilization. In recent years, stem-cell therapy has arisen as a new hope in the field of reproductive disabili...
Source: Current Molecular Medicine - July 9, 2019 Category: Molecular Biology Authors: Wang J, Liu C, Fujino M, Tong G, Zhang Q, Li XK, Yan H Tags: Curr Mol Med Source Type: research

Novel Insight into the Differential Gene Expression and Clinical Significance of Dopamine Receptors, COMT, and IL6 in BPH and Prostate Cancer.
CONCLUSION: Results implicate a potential cross- talk between the signaling pathways derived by IL6 cytokine and dopamine receptors in PCa. Thus it seems promising to re- assemble the consequent signaling pathways by adequate agonists and antagonists to help increase therapeutic efficacy. PMID: 31288722 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - July 9, 2019 Category: Molecular Biology Authors: Akbarian F, Abolhasani M, Dadkhah F, Asadi F, Ahangari G Tags: Curr Mol Med Source Type: research

Expression suppression and activity inhibition of TRPM7 regulates cytokine production and multiple organ dysfunction syndrome during endotoxemia: A new target for sepsis.
CONCLUSION: Data presented here points TRPM7 ion channel as a key protein regulating inflammatory response and MODS during sepsis. Noteworthy, TRPM7 appears as a novel molecular target for the management of sepsis. PMID: 31288723 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - July 9, 2019 Category: Molecular Biology Authors: Gatica S, Eltit F, Santibanez JF, Varela D, Cabello-Verrugio C, Simon F Tags: Curr Mol Med Source Type: research

Amiloride alleviates neurological deficits following transient global ischemia and engagement of central IL-6 and TNF- α signal.
CONCLUSION: Transient global ischemia induced by CA amplifies ASIC1a in the hippocampus likely via PIC signal. Amiloride administered into the central nerve system plays a neuroprotective role in the pathophysiological process of global ischemia. Thus, targeting ASICs (i.e., ASIC1a) has clinical implications for treatment and improvement of CA-evoked global cerebral ischemia often observed in clinics. PMID: 31272354 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - July 3, 2019 Category: Molecular Biology Authors: Pang L, Ji S, Xing J Tags: Curr Mol Med Source Type: research

The Effect of Interleukin 38 on Inflammation-induced Corneal Neovascularization.
In this study, we examined the role of IL-38, a member of IL-1 family, in mediating inflammation-induced angiogenesis. The results showed that the angiogenesis was attenuated by topical administration of IL-38 to the injured corneas in a mouse model of alkali-induced corneal neovascularization (CNV). Further study showed that the expression of inflammatory cytokines TNF-a, IL-6, IL-8 and IL-1b was decreased in the IL-38-treated corneas. Moreover, the angiogenic activities including the proliferation, migration and tube formation of human retinal endothelial cells was reduced by IL-38 treatment in vitro. The data indicate t...
Source: Current Molecular Medicine - June 27, 2019 Category: Molecular Biology Authors: Zhu J, Zhang J, Wang Y, Chen J, Li X, Liu X, Kong E, Su SB, Zhang Z Tags: Curr Mol Med Source Type: research

Role of polymorphisms of FAM13A, PHLDB1, and CYP24A1 in breast cancer risk.
CONCLUSION: Our results suggest that rs1059122 (FAM13A), rs17748 (PHLDB1), and rs4809957 (CYP24A1) may play an important role in the development of breast cancer in the Chinese Han population. Future studies will identify both population replication and functional validation to confirm our findings. PMID: 31215377 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - June 19, 2019 Category: Molecular Biology Authors: Wei Y, Wang X, Zhang Z, Xie M, Li Y, Cao H, Zhao X Tags: Curr Mol Med Source Type: research

Diabetic hemodialysis: vitamin D supplementation and its related signaling pathways involved in insulin and lipid metabolism.
CONCLUSIONS: Our findings confirmed, diabetic HD subjects who were received the vitamin D supplementation (for 12 weeks), show a significant over expression in the PPAR-γ, AKT, PI3K, IRS1 and GLUT4 genes, and also show a significant down regulation in the PKC and LDLR genes. Moreover, did not observe any effects on PDK1, IRS2 and Lp(a) expression. PMID: 31210105 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - June 18, 2019 Category: Molecular Biology Authors: Hosseini ES, Kashani HH, Nikzad H, Soleimani A, Mirzaei H, Tamadon MR, Asemi Z Tags: Curr Mol Med Source Type: research

Risk Factor for Residue After Uterine Artery Chemotherapy and Embolization in Combination with Dilatation and Curettage for Treating Caesarean Scar Pregnancy.
CONCLUSIONS: In cases with GS evagination to bladder and HCG of>28,113.65mIU/ml as well as a gestational age of>28 days, the possibility of residual lesions was high. PMID: 31195943 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - June 12, 2019 Category: Molecular Biology Authors: Cheng F, Shan D, Guo S, Cheng S, Yang H, Han J, Hu T Tags: Curr Mol Med Source Type: research

Homocysteine in hepatocellular carcinoma with portal vein thrombosis.
era M Abstract BACKGROUND: Portal vein thrombosis (PVT) is a common complication of end-stage hepatocellular carcinoma (HCC). Many factors may contribute to the pathogenesis. The aim of our study was to evaluate the role of Homocysteine (Hcy) in HCC patient with PVT. Hcy is a sulphur amino-acid involved in two pathways, trans-sulphuration and remethylation, that involve vitamins B6, B12 and folates. METHODS: We recruited 54 patients with HCC and PVT, 60 patients with HCC and without PVT and 60 control subjects. We measured serum levels of Hcy and vitamin B6 after protein precipitation and derivation using an ...
Source: Current Molecular Medicine - June 10, 2019 Category: Molecular Biology Authors: Latteri S, Malaguarnera G, Catania VE, La Greca G, Bertino G, Borzì AM, Drago F, Malaguarnera M Tags: Curr Mol Med Source Type: research

Isothermal amplification methods for the SNP genotyping.
Abstract The demands for genotyping techniques with acceptable precision, accuracy, cost effectiveness in high throughput formats made driving forces for continuously development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced. The best alternatives were the isothermal amplification technologies that those did not require a thermal cycler. In this review, we aimed to describe the most known isothermal amplification techniques for SNP genotyping. PMID: 31131752 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - May 26, 2019 Category: Molecular Biology Authors: Sharafdarkolaee SH, Gill P, Motovali-Bashi M, Sharafdarkolaee FH Tags: Curr Mol Med Source Type: research

Repurposing of Fluvastatin against Candida albicans CYP450 lanosterol 14 α-demethylase, a target enzyme for antifungal therapy: An In silico and In vitro study.
CONCLUSION: However, further, in vivo studies are required to confirm the complete role of fluvastatin as an antifungal agent. PMID: 31109273 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - May 19, 2019 Category: Molecular Biology Authors: Rana R, Sharma R, Kumar A Tags: Curr Mol Med Source Type: research

MiR-137 restricts viability and migration of HTR-8/SVneo cells by downregulating FNDC5 in gestational diabetes mellitus.
CONCLUSION: These data suggest that miR-137 suppresses viability and migration of trophoblasts via downregulating FNDC5 in GDM, which might contribute to pathology of placenta tissues and occurrence of adverse pregnancy outcomes. PMID: 31109274 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - May 19, 2019 Category: Molecular Biology Authors: Peng HY, Li MQ, Li HP Tags: Curr Mol Med Source Type: research

Molecular Investigations of the New Synthesized Azomethines as Antioxidants: Theoretical and Experimental Studies.
Abstract Density functional theory (DFT) was employed to investigate the Bond Dissociation Enthalpy (BDE), Mulliken Charges, NBO analysis, Ionization Potential (IP), Electron Affinities (EA), HOMO and LUMO energies, Hardness (η), Softness (S), Electronegativity (μ), Electrophilic Index (ω), Electron Donating Power (ω-), Electron Accepting Power (ω+) and Energy Gap (Eg) in order to deduce scavenging action of the two new synthesized azomethines: 2,2'-((1E,1'E)-(1,4-phenylenebis(azanylylidene))bis(methanylylidene))diphenol (FD-1) 4,4'-((1E,1'E)-(1,4-phenylenebis(azanylylidene))bis(methanylyl...
Source: Current Molecular Medicine - May 8, 2019 Category: Molecular Biology Authors: Shahab S, Sheikhi M, Filippovich L, Dikusar E, Rouhani M, Pazniak A, Kumar R Tags: Curr Mol Med Source Type: research

BNIP3-mediated autophagy induced inflammatory response and inhibited VEGF expression in cultured retinal pigment epithelium cells under hypoxia.
Conclusion: BNIP3-mediated autophagy induced by hypoxia may regulate inflammatory response and VEGF expression, which can affect the cell viability of RPE under hypoxia. PMID: 31072291 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - May 8, 2019 Category: Molecular Biology Authors: Chen Y, Yan Q, Xu Y, Ye F, Sun X, Zhu H, Wang H Tags: Curr Mol Med Source Type: research

Investigation of the Adsorption Rubraca Anticancer Drug on the CNT(4,4-8) Nanotube as a Factor of Drug Delivery: A Theoretical Study Based on DFT Method.
Abstract In the present study, the interaction between new drug Rubraca and CNT(4,4-8) nanotube by Density Functional Theory (DFT) calculations in an aqueous medium for first time have been investigated. According to calculations, the intermolecular hydrogen bonds take place between active positions of the molecule Rubraca and hydrogen atoms of the nanotube that plays an important role in the stability of the complex CNT(4,4-8)/Rubraca. The non-bonded interaction effects of the molecule Rubraca with CNT(4,4-8) nanotube on the electronic properties, chemical shift tensors and natural charge have been also detected....
Source: Current Molecular Medicine - May 6, 2019 Category: Molecular Biology Authors: Sheikhi M, Shahab S, Khaleghian M, Ahmadianarog M, Kumar R Tags: Curr Mol Med Source Type: research

Dopaminergic genes polymorphisms and prefrontal cortex efficiency among obese people - whether gender is a differentiating factor?
In this study we assessed the association between dopaminergic gene polymorphisms: DAT1 (SLC6A3), COMTVal158Met, DRD4 (48-bp variable number of terminal repeats - VNTR) and WCST parameters to investigate the frontal lobes functions in obese indivuduals. Polymorphisms of DAT1, COMTVal158Met, DRD4 may influence executive functions in the obese subjects. The analysis of the frequency of individual alleles of the studied genetic polymorphisms was performed in 248 obese patients (179 women, 69 men). Evaluation of the prefrontal cortex function (operating memory and executive functions) was performed using the Wisconsin Card Sor...
Source: Current Molecular Medicine - April 24, 2019 Category: Molecular Biology Authors: Bieliński M, Lesiewska N, Junik R, Kamińska A, Tretyn A, Borkowska A Tags: Curr Mol Med Source Type: research

Genetic Alleles Associated with SLE Susceptibility and Clinical Manifestations in Hispanic Patients from the Dominican Republic.
CONCLUSION: Our data suggest that the Latin American population shares some common genetic risk factors for SLE as other populations, but also has distinct risk gene alleles that contribute to SLE susceptibility and development of LN and NPSLE. This is the first study focusing on genetic risk factors for SLE in the DR, a Latin American population that has never been characterized before. PMID: 31032751 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 24, 2019 Category: Molecular Biology Authors: Liu Z, Yu Y, Yue Y, Hearth-Holmes M, Lopez PD, Tineo C, Paulino G, Fu WN, Loyo E, Su K Tags: Curr Mol Med Source Type: research

Celastrol Protects RPE Cells from Oxidative Stress-Induced Cell Death via Activation of Nrf2 Signaling Pathway.
CONCLUSION: This study provides evidence that treatment of RPE cells with celastrol shows potent protective effects against oxidative insults via activation of Nrf2 signaling pathway and upregulation of GCLM expression. This finding suggests that celastrol might be used as a potential therapeutic agent for oxidative stress-related eyes diseases, such as AMD. PMID: 31032752 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 24, 2019 Category: Molecular Biology Authors: Zhou Y, Zhou L, Zhou K, Zhang J, Shang F, Zhang X Tags: Curr Mol Med Source Type: research

Gentianella turkestanerum showed protective effects on hepatic injury by modulating the endoplasmic reticulum stress and NF- κB signaling pathway.
Gentianella turkestanerum showed protective effects on hepatic injury by modulating the endoplasmic reticulum stress and NF-κB signaling pathway. Curr Mol Med. 2019 Apr 15;: Authors: Yang J, Zhu D, Wen L, Xiang X, Hu J Abstract OBJECTIVE: To investigate the protective effects of Gentianella turkestanerum extraction by butanol (designated as GBA) on hepatic cell line L02 injury induced by carbon tetrachloride (CCl4) and hydrogen peroxide (H2O2). METHODS: L02 cells were incubated with 5 μg/mL, 10 μg/mL, 20 μg/mL, 40 μg/mL, 60 μg/mL, 80 μg/mL and 100 μg/mL GBA for 24 hours...
Source: Current Molecular Medicine - April 15, 2019 Category: Molecular Biology Authors: Yang J, Zhu D, Wen L, Xiang X, Hu J Tags: Curr Mol Med Source Type: research

Occurrence of Interleukin-2 (330 G/T) Promoter Polymorphism in ARV associated hepatotoxicity.
CONCLUSION: Thus, IL-2-303G/T polymorphism did not confer the susceptibility to ARV associated hepatotoxicity. However, IL-2-303G/T polymorphism with nevirapine usage may facilitate the risk for acquisition of ARV associated hepatotoxicity. PMID: 30973108 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 10, 2019 Category: Molecular Biology Authors: Singh H, Nambiar N, Samani D, Gangakhedkar RR Tags: Curr Mol Med Source Type: research

Association of Vitamin D Gene Polymorphisms and Bone Mineral Density in Healthy young Saudi Females.
CONCLUSION: The presence of the TT allele of the SNP rs2228570 of the VDR gene and the SNP rs731236 of the CC genotype were associated with the presence of osteopenia and decreased bone mineral density alongside malfunctions of vitamin D. PMID: 30963970 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 9, 2019 Category: Molecular Biology Authors: Taha IM, Allah AMA, El Tarhouny S Tags: Curr Mol Med Source Type: research

Axonopathy likely initiates neuropathological processes via a mechanism of axonal leakage in Alzheimer's mouse models.
CONCLUSION: These data provide an explanation for the common origin and development of SPs and NFTs, and suggest that axonal leakage might be a key event in the development of the neuropathological processes in AD. PMID: 30961496 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 5, 2019 Category: Molecular Biology Authors: Gao G, He J, Luo Y, Sun Y, Zhou Y, Zhang J, Xing Y, Dai J Tags: Curr Mol Med Source Type: research

FoxF1 is Required for Ciliogenesis and Distribution of Sonic Hedgehog Signaling Components in Cilium.
CONCLUSION: Together, our data illustrated that FoxF1 is required for ciliogenesis in vitro and in vivo and for the proper localization of Shh signaling components in cilium. PMID: 30950350 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 5, 2019 Category: Molecular Biology Authors: Huang L, Tjakra M, Luo D, Wen L, Lei D, Wang J, Yin T, Zhang X, Huang J, Wang Y, Wang G Tags: Curr Mol Med Source Type: research

Serum IL-33 level and IL-33, IL1RL1 gene polymorphisms in asthma and multiple sclerosis patients.
This study consisted of asthma (n=140) and MS patients (n=140), and healthy subjects (n=72). Genotyping was carried out in 2 genetic polymorphisms, rs1342326 variant of IL-33 and rs10204137 SNP of IL-33 receptor genes, using High Resolution Melt Real Time PCR based method. The level of serum IL-33 also was measured using enzyme-linked immunosorbent assay method. RESULTS: The level of IL33 was significantly higher in asthma and MS patients compared to control group (P
Source: Current Molecular Medicine - April 5, 2019 Category: Molecular Biology Authors: Ahmadi M, Fathi F, Fouladi S, Alsahebfosul F, Manian M, Eskandari N Tags: Curr Mol Med Source Type: research

Human Papillomavirus Genome based Detection and Typing: A Holistic Molecular Approach.
Abstract Human Papillomavirus (HPV) is a species specific double-stranded DNA virus infecting human cutaneous or mucosal tissues. The genome structure of HPV is extremely polymorphic hence making it difficult to discriminate between them. HPV exhibits numerous dissimilar types that can be subdivided into high-risk (HR), probably high-risk and low-risk (LR), causing numerous types of cancers and warts around the genital organs in humans. Several screening methods are performed in order to detect cytological abnormalities and presence or absence of HPV genome. Currently available commercial kits and methods are desi...
Source: Current Molecular Medicine - April 5, 2019 Category: Molecular Biology Authors: Gautam A, Gedda MR, Rai M, Sundar S, Chakravarty J Tags: Curr Mol Med Source Type: research

Inhibition of SOX15 Sensitizes Esophageal Squamous Carcinoma Cells to Paclitaxel.
In conclusion, the current study revealed that inhibition of SOX15 in ESCC cells sensitizes the ESCC cells to paclitaxel, suggesting that the SOX15 expression level may predict the therapeutic outcomes for paclitaxel treatment for ESCC. PMID: 30950353 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 5, 2019 Category: Molecular Biology Authors: Zhang M, Wang J, Gao T, Chen X, Xu Y, Yu X, Guo X, Zhuang R, Li Z, Wu H, Yu J Tags: Curr Mol Med Source Type: research

Intricate Regulation of Phosphoenolpyruvate Carboxykinase (PEPCK) Isoforms in Normal Physiology and Disease.
CONCLUSION: Based on current knowledge and extensive bioinformatics analysis, in this review we have provided a comparative (epi)genetic understanding of PCK1 and PCK2 genes encompassing regulatory elements, disease associated polymorphisms, copy number variations, regulatory miRNAs and CpG densities. We have also discussed various exogenous and endogenous modulators of PEPCK isoforms and their signaling mechanisms. Comprehensive review of existing knowledge of PEPCK regulation and function may enable identification of the underlying gaps to design new pharmacological strategies and interventions for the diseases associate...
Source: Current Molecular Medicine - April 4, 2019 Category: Molecular Biology Authors: Seenappa V, Joshi MB, Satyamoorthy K Tags: Curr Mol Med Source Type: research

Revealing synergistic mechanism of multiple components in compound Fengshiding capsule for rheumatoid arthritis therapeutics by network pharmacology.
Abstract Compound Fengshiding capsule (CFC), is a Chinese formulation from herbal origin including Alangium platanifolium, Angelicae dahurica, Cynanchum paniculatum and Glycyrrhiza uralensis. CFC is widely used as clinical therapy against rheumatoid arthritis. However, its exact mechanism of action has not been explored yet. In order to explore the synergistic mechanism of CFC, we designed a study adopting network pharmacology scheme to screen the action targets in relation to the CFC components. The study analyses target facts of salicin, paeonol, liquiritin and imperatorin from PubMed database, and explores the ...
Source: Current Molecular Medicine - April 4, 2019 Category: Molecular Biology Authors: Duan H, Zhai KF, Khan GJ, Zhou J, Cao TY, Wu YQ, Zhou YR, Cao WG, Gao GZ, Shan LL Tags: Curr Mol Med Source Type: research

Assessment of the effect of baicalin on duck virus hepatitis.
CONCLUSION: This study may provide a scientific basis for developing baicalin as one or a part of the anti-DHAV-1 drugs. PMID: 30950349 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - April 4, 2019 Category: Molecular Biology Authors: Chen Y, Yao F, Ming K, Shi J, Zeng L, Wang D, Wu Y, Hu Y, Liu J Tags: Curr Mol Med Source Type: research

Identifying the potential substrates of the depalmitoylation enzyme Acyl-protein thioesterase 1.
CONCLUSION: Our study provided a large scale of potential substrates of APT1 and thus shall facilitate the understanding of its intervened molecular functions. PMID: 30914023 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - March 25, 2019 Category: Molecular Biology Authors: Liu H, Yan P, Ren J, Wu C, Yuan W, Rao M, Zhang Z, Kong E Tags: Curr Mol Med Source Type: research

Molecular chaperone HSP70 and key regulators of apoptosis.
Abstract Identified as a molecular chaperone constitutively being synthesized due to enhanced elevated temperature change, this heat shock protein HSP70 has shown to be intimately involved in many protein biogenesis, facilitating the synthesis and folding of proteins and trafficking of nascent peptides during cell growth. HSP70 also plays a vital role in protein assembly, regulation and interaction with a wide variety of proteins. Stress-induced cell death is under the control of the Bcl-2 family of apoptotic regulators and display either pro-apoptotic or anti-apoptotic activities. Subjected to stress conditions s...
Source: Current Molecular Medicine - March 25, 2019 Category: Molecular Biology Authors: Roufayel R Tags: Curr Mol Med Source Type: research

A Revolution in Reprogramming: Small Molecules.
Abstract Transplantation of reprogrammed cells from accessible sources and in vivo reprogramming are potential therapies for regenerative medicine. During the last decade, genetic approaches, which mostly involved transcription factors and microRNAs, have been shown to affect cell fates. However, their potential carcinogenicity and other unexpected effects limit their translation into clinical applications. Recently, with the power of modern biology-oriented design and synthetic chemistry, as well as high-throughput screening technology, small molecules have been shown to enhance reprogramming efficiency, replace ...
Source: Current Molecular Medicine - March 24, 2019 Category: Molecular Biology Authors: Zhou J, Sun J Tags: Curr Mol Med Source Type: research

Integrons and antiseptic resistance genes mediate resistance of Acinetobacter baumannii and Pseudomonas aeruginosa isolates from intensive care unit patients with wound infections.
Abstract Acinetobacter baumannii and Pseudomonas aeruginosa are of major concern for hospitalized patients. We evaluated antibiotic and antiseptic resistance of A. baumannii (n = 29) and P. aeruginosa (n = 37) isolates recovered from 66 intensive care unit (ICU) patients and determined the prevalence of qacE, qacEΔ1, and integrons in these clinical isolates. Antibiotic and antiseptic susceptibility testing were performed via Kirby Bauer disk diffusion and broth microdilution methods, respectively. The resistance genes and integrons were detected by PCR. A. baumannii and P. aeruginosa ICU isolates showed 100%...
Source: Current Molecular Medicine - March 20, 2019 Category: Molecular Biology Authors: Elkhatib WF, Khalil MAF, Ashour HM Tags: Curr Mol Med Source Type: research

Attenuation of cardiac ischaemia-reperfusion injury by treatment with hydrogen-rich water.
This study examined the signalling pathways for hydrogen attenuating ischaemia-reperfusion injury. In total, 20 male Wistar rats were evaluated for the effects of hydrogen-rich water on ischaemia-reperfusion in hearts. Left ventricular tissue was taken for screening and analysis of active protein factors by protein chip technology. The enrichment of the KEGG pathway was obtained by using the Gene Ontology (GO) enrichment principle. The expression of JAK2、STAT1、STAT3、p-STAT1、p-JAK2、p-STAT3 in rat myocardium was detected by Western blot analysis and immunohistochemistry. The apoptosis rates of the control and hydro...
Source: Current Molecular Medicine - March 20, 2019 Category: Molecular Biology Authors: Li X, Li L, Liu X, Wu J, Sun X, Li Z, Geng YJ, Liu F, Zhou Y Tags: Curr Mol Med Source Type: research

Berberine alleviates amyloid-beta pathogenesis via activating LKB1/AMPK signaling in the brain of APP/PS1 transgenic mice.
Abstract BACKGROUND: Liver kinase B1 (Background: LKB1)/5'-adenosine monophosphate-activated protein kinase (AMPK) signaling, a metabolic checkpoint, plays a neuro-protective role in the pathogenesis of Alzheimer's disease (AD). Amyloid-β (Aβ) acts as a classical biomarker of AD. The aim of the present study was to explore whether berberine (BBR) activates LKB1/AMPK signaling and ameliorates Aβ pathology. METHODS: The Aβ levels were detected using enzyme -linked immunosorbent assay and immunohistochemistry. The following biomarkers were measured by Western blotting: phosphorylated (p-) LKB...
Source: Current Molecular Medicine - March 15, 2019 Category: Molecular Biology Authors: Cai ZY, Wang CL, Lu TT, Yang WM Tags: Curr Mol Med Source Type: research

Blood Circulating Exosomes Contain Distinguishable Fractions of Free and Cell-Surface-Associated Vesicles.
CONCLUSION: Our data provide the basis for using blood cell-associated exosomes for diagnostic applications. PMID: 30868953 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - March 14, 2019 Category: Molecular Biology Authors: Tamkovich S, Tutanov O, Efimenko A, Grigor'eva A, Ryabchikova E, Kirushina N, Vlassov V, Tkachuk V, Laktionov P Tags: Curr Mol Med Source Type: research

Diagnostic Value of MiR-125b as a Potential Biomarker for Stage I Lung Adenocarcinoma Lung Adenocarcinoma.
Tekin L, Erdal ME Abstract BACKGROUND: We aimed to explore biological functions of differentially expressed miRNAs during carcinogenesis, to identify miRNAs dysegulations involved in DNA repair mechanisms, and to evaluate potential of miRNAs as prognostic and diagnostic biomarkers for early lung adenocarcinomas (LAC). METHODS: We obtained 21 LAC and paired adjacent normal formalin-fixed, paraffin-embedded lung tissues from patients who underwent curative resection for stage I LAC. We compared expression levels of eight miRNAs involved in DNA repair mechanism between LAC and adjacent tissues. RESULTS: Exp...
Source: Current Molecular Medicine - March 13, 2019 Category: Molecular Biology Authors: Zeybek A, Öz N, Kalemci S, Edgünlü T, Kızıltuğ M, Tosun K, Tunç M, Tekin L, Erdal ME Tags: Curr Mol Med Source Type: research

Bone Metabolism Markers in Thalassemia Major-Induced Osteoporosis: Results from a Cross-Sectional Observational Study.
CONCLUSION: Patients with TM do not have a higher probability of suffering from osteoporosis from the general population. However, some markers of osteoclast activity differ between patients with TM and osteoporosis, indicating possibly differences in terms of anti-osteoporotic treatment. The lack of significant differences among the three groups in regards to the levels of CTX and sclerostin may indicate the potential efficacy of the current osteoporotic treatment also for TM patients. PMID: 30868952 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - March 13, 2019 Category: Molecular Biology Authors: Tsartsalis AN, Lambrou GI, Tsartsalis DN, Papassotiriou I, Vlachou E, Terpos E, Chrousos GP, Kanaka-Gantenbein C, Kattamis A Tags: Curr Mol Med Source Type: research

Adeno-associated virus 9-mediated small RNA interference of TLR4 alleviates myocardial ischemia and reperfusion injury by inhibition of the NF- κB and MAPK signaling pathways in rats.
Adeno-associated virus 9-mediated small RNA interference of TLR4 alleviates myocardial ischemia and reperfusion injury by inhibition of the NF-κB and MAPK signaling pathways in rats. Curr Mol Med. 2019 Mar 11;: Authors: Xu T, Zhang K, Kan F, Li F, Yu B, Du W, Nie H Abstract Background Despite intensive investigation, effective therapeutic procedures for myocardial I/R injury are still in demand. OBJECTIVE: To explore the effect of adeno-associated virus 9 (AAV9)-mediated small interfering RNA targeting TLR4 in the treatment of myocardial ischemia and reperfusion (I/R) injury and its influen...
Source: Current Molecular Medicine - March 11, 2019 Category: Molecular Biology Authors: Xu T, Zhang K, Kan F, Li F, Yu B, Du W, Nie H Tags: Curr Mol Med Source Type: research

Glucose Oxidase- and UVA-Induced Changes in the Expression Patterns of Seven De-sumoylation Enzymes (SENPs) Are Associated with Cataract Development.
CONCLUSION: Our results for the first time established the differentiation expression patterns of the 7 de-sumoylation enzymes (SENPs) under treratment by GO or UVA, which provides preliminary data to link sumoylation to stress-induced cataractogenesis. PMID: 30854967 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - March 10, 2019 Category: Molecular Biology Authors: Xiang JW, Xiao Y, Gan Y, Chen H, Liu Y, Wang L, Nie Q, Liu F, Gong X, Fu JL, Qing WJ, Yang L, Xie J, Luo Z, Qi R, Chen Z, Li DW Tags: Curr Mol Med Source Type: research

The Mitochondrial tRNAHis G12192A Mutation May Modulate the Clinical Expression of Deafness-Associated tRNAThr G15927A Mutation in a Chinese Pedigree.
CONCLUSION: Our data indicated that mt-tRNAHis G12192A mutation may increase the penetrance and expressivity of deafness-associated m-tRNAThr G15927A mutation in this family. PMID: 30854964 [PubMed - as supplied by publisher] (Source: Current Molecular Medicine)
Source: Current Molecular Medicine - March 8, 2019 Category: Molecular Biology Authors: Ding Y, Teng YS, Zhuo GC, Xia BH, Leng JH Tags: Curr Mol Med Source Type: research