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Condition: Asthma

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Total 404 results found since Jan 2013.

Calcium-sensing receptor and CPAP-induced neonatal airway hyperreactivity in mice
CONCLUSIONS: These data implicate CaSR in CPAP effects on airway function with implications for wheezing in former preterm infants.IMPACT: Neonatal CPAP increases airway reactivity to bronchoconstrictor agonist. CPAP increases smooth muscle expression of the extracellular calcium-sensing receptor (CaSR). Inhibition or absence of CaSR blunts CPAP effects on contractility. These data suggest a causal/contributory role for CaSR in stretch effects on the developing airway. These data may impact clinical recognition of the ways that CPAP may contribute to wheezing disorders of former preterm infants.PMID:33958714 | DOI:10.1038/s41390-021-01540-4
Source: Pediatric Research - May 7, 2021 Category: Pediatrics Authors: Catherine A Mayer Benjamin Roos Jacob Teske Natalya Wells Richard J Martin Wenhan Chang Christina M Pabelick Y S Prakash Peter M MacFarlane Source Type: research

The Degradation of Airway Epithelial Tight Junctions in Asthma Under High Airway Pressure Is Probably Mediated by Piezo-1
Full functioning of the airway physical barrier depends on cellular integrity, which is coordinated by a series of tight junction (TJ) proteins. Due to airway spasm, edema, and mucus obstruction, positive end-expiratory alveolar pressure (also termed auto-PEEP) is a common pathophysiological phenomenon, especially in acute asthma attack. However, the influence of auto-PEEP on small airway epithelial TJs is currently unclear. We performed studies to investigate the effect of extra pressure on small airway epithelial TJs and its mechanism. The results first confirmed that a novel mechanosensitive receptor, piezo-1, was highl...
Source: Frontiers in Physiology - April 1, 2021 Category: Physiology Source Type: research

Effect of TLR7 gene expression mediating NF- κB signaling pathway on the pathogenesis of bronchial asthma in mice and the intervention role of IFN-γ.
CONCLUSIONS: Upregulation of TLR7 can inhibit the activation of NF-κB signaling pathway, reduces airway inflammation, inhibits ASMCS proliferation and thus promotes cell apoptosis in asthmatic mice. Besides, IFN-γ can exert a protective role in suppressing the progression of inflammation in asthma. PMID: 33577041 [PubMed - as supplied by publisher]
Source: European Review for Medical and Pharmacological Sciences - February 14, 2021 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

S1PR2 Inhibition Attenuates Allergic Asthma Possibly by Regulating Autophagy
This study is to investigate the role of Sphingosine-1-phosphate (S1P) in the asthma progression, and the involvement of autophagy. Airway remodeling mice were subjected to the HE, PAS, and Masson staining. Protein expression levels in the tissues, samples and model cells were detected with ELISA, Western blot analysis, and immunohistochemical/immunofluorescent analysis. The S1P2 receptor antagonist JTE-013 decreased the inflammatory cell infiltration and goblet cell production in asthmatic mice tissues. The IL-1, IL-4, IL-5 and serum IgE contents were decreased in bronchoalveolar lavage fluid, while the Beclin1 expression...
Source: Frontiers in Pharmacology - February 10, 2021 Category: Drugs & Pharmacology Source Type: research

Cellular Clocks in Hyperoxia Effects on Ca2+i Regulation in Developing Human Airway Smooth Muscle.
We report that human fASM express core clock machinery (PER1, PER2, CRY1, ARNTL/BMAL1, CLOCK) that is responsive to dexamethasone and altered by O2. Disruption of the clock via siRNA-mediated PER1 or ARNTL knockdown alters store-operated calcium entry (SOCE) and [Ca2+]i response to histamine in hyperoxia. Effects of O2 on [Ca2+]i are rescued by driving expression of clock proteins, via effects on the Ca2+ channels IP3R and Orai1. These data reveal a functional fASM clock that modulates [Ca2+]i regulation, particularly in hyperoxia. Harnessing clock biology may be a novel therapeutic consideration for neonatal airway diseas...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - January 6, 2021 Category: Cytology Authors: Bartman CM, Matveyenko A, Pabelick CM, Prakash YS Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

GSE146017 Influence of CEBPD  on Glucocorticoid Response in Airway Smooth Muscle 
Contributor : Blanca E HimesSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCCAAT/Enhancer Binding Protein D (CEBPD) is a transcription factor that regulates genes involved in immune and inflammatory responses. Based on our previous observation that CEBPD expression increases in airway smooth muscle (ASM) with glucocorticoid exposure, we sought to better understand its role in the ASM transcriptomic response to glucocorticoids via knockdown experiments.Primary human airway smooth muscle (ASM) cells from four non-asthma donors were transfected with 25pmol of siRNA non-targeting control...
Source: GEO: Gene Expression Omnibus - December 31, 2020 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Molecules, Vol. 25, Pages 6028: The Antiasthma Medication Ciclesonide Suppresses Breast Cancer Stem Cells through Inhibition of the Glucocorticoid Receptor Signaling-Dependent YAP Pathway
This study focused on investigating the effect of ciclesonide on breast cancer and CSCs and determining its underlying mechanism. Here, we showed that ciclesonide inhibits breast cancer and CSC formation. Similar glucocorticoids—dexamethasone and prednisone—did not inhibit CSC formation. Ciclesonide-induced glucocorticoid receptor (GR) degradation was dependent on ubiquitination. We showed via GR small interfering RNA (siRNA) that GR plays an important role in CSC formation. We showed via western blot and immunofluorescence assays that ciclesonide reduces the nuclear level of GR. The GR antagoni...
Source: Molecules - December 19, 2020 Category: Chemistry Authors: Su-Lim Kim Hack Sun Choi Ji-Hyang Kim Dong-Sun Lee Tags: Article Source Type: research

siRNA delivery to macrophages using aspherical, nanostructured microparticles as delivery system for pulmonary administration.
Abstract The delivery of oligonucleotides such as siRNA to the lung is a major challenge, as this group of drugs has difficulties to overcome biological barriers due to its polyanionic character and the associated hydrophilic properties, resulting in inefficient delivery. Especially in diseases such as asthma, chronic obstructive pulmonary disease and cystic fibrosis, where increased proinflammation is present, a targeted RNA therapy is desirable due to the high potency of these oligonucleotides. To address these problems and to ensure efficient uptake of siRNA in macrophages, a microparticulate, cylindrical deliv...
Source: European Journal of Pharmaceutics and Biopharmaceutics - December 4, 2020 Category: Drugs & Pharmacology Authors: Fischer T, Tschernig T, Drews F, Brix K, Meier C, Simon M, Kautenburger R, Schneider M Tags: Eur J Pharm Biopharm Source Type: research

TNF α Induces Mitochondrial Fragmentation and Biogenesis in Human Airway Smooth Muscle.
TNFα Induces Mitochondrial Fragmentation and Biogenesis in Human Airway Smooth Muscle. Am J Physiol Lung Cell Mol Physiol. 2020 Nov 04;: Authors: Delmotte PF, Marin Mathieu N, Sieck GC Abstract In human airway smooth muscle (hASM), mitochondrial volume density is greater in asthmatic patients compared to normal controls. There is also an increase in mitochondrial fragmentation in hASM of moderate asthmatics associated with an increase in Drp1 and a decrease in Mfn2 expression, mitochondrial fission and fusion proteins, respectively. Pro-inflammatory cytokines such TNFα contribute to hASM hyperreacti...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - November 4, 2020 Category: Cytology Authors: Delmotte PF, Marin Mathieu N, Sieck GC Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

Respiratory syncytial virus upregulates IL-33 expression in mouse model of virus-induced inflammation exacerbation in OVA-sensitized mice and in asthmatic subjects.
CONCLUSION: The present study provides additional evidence of the crucial role of the IL-33 in pathogenesis of RSV infection and virus-induced allergic bronchial asthma exacerbations. PMID: 33132030 [PubMed - as supplied by publisher]
Source: Cytokine - October 29, 2020 Category: Molecular Biology Authors: Nikonova A, Shilovskiy I, Galitskaya M, Sokolova A, Sundukova M, Dmitrieva-Posocco O, Mitin A, Komogorova V, Litvina M, Sharova N, Zhernov Y, Kudlay D, Dvornikov A, Kurbacheva O, Khaitov R, Khaitov M Tags: Cytokine Source Type: research

Thrombin induces stomatin-like protein 2 (STOML2) expression and IL-8/CXCL8 release by ROCK, JNK, and PEA3 pathways in human lung epithelial cells
In this study, we investigate whether Rho kinase (ROCK), JNK, and PEA3 participate in thrombin-induced STOML2 expression and IL-8/CXCL8 release in human lung epithelial cells. We found that thrombin induced increase in STOML2, but not prohibitin, expression in a time-dependent manner. Thrombin also induces STOML2-luciferase activity. Thrombin-induced STOML2 expression and IL-8/CXCL8 release were reduced by PEA3 siRNA. We also found that transfection of A549 cells with PEA3 siRNA reduced PEA3 protein expression. In addition, thrombin induced increase in PEA3 Ser phosphorylation and PEA3 translocation from the cytosol to the...
Source: European Respiratory Journal - October 28, 2020 Category: Respiratory Medicine Authors: Chen, B.-C., Lin, C.-H. Tags: Airway pharmacology and treatment Source Type: research

Cbx4 Regulates RNA Binding Protein MEX-3B To Thymic stromal lymphopoietin Expression Sumoylation In Epithelium Of HDM-induced Allergic Inflammation
Conclusion: CBX4/MEX-3B/TSLP axis involved in TSLP-mediated airway inflammation,suggesting that targeting SUMO E3 ligase activity of CBX4 would be a novel target for the treatment of asthma.
Source: European Respiratory Journal - October 28, 2020 Category: Respiratory Medicine Authors: shixiu, l., Changhui, Y., Zicong, Z., Zili, Z., Jieyi, L., Shaoxi, C. Tags: Allergy and immunology Source Type: research

Recombinant Pyrin Domain Protein Attenuates Airway Inflammation and Alleviates Epithelial-Mesenchymal Transition by Inhibiting Crosstalk Between TGF β1 and Notch1 Signaling in Chronic Asthmatic Mice
In conclusion, RPYD reduces airway inflammation, improves airway remodeling and reduces EMT in chronic asthmatic mice by inhibiting the crosstalk between TGFβ1/smad3 and Jagged1/Notch1 signaling pathways.
Source: Frontiers in Physiology - October 23, 2020 Category: Physiology Source Type: research

TMEM16A Mediated Mucus Production in Human Airway Epithelial Cells.
We examined this question using BCi-NS1 cells, a human airway basal cell line that maintains multipotent differentiation capacity, and the two human airway epithelial cell lines, Calu-3 and CFBE. The data demonstrate that exposure of airway epithelial cells to IL-8 and IL-13, two cytokines known to be enhanced in CF and asthma, respectively, leads to increase in mucus production. Expression of MUC5AC was fully dependent on expression of TMEM16A, as shown by siRNA-knockdown of TMEM16A. In addition, different inhibitors of TMEM16A attenuated IL-13 induced mucus production. Interestingly, in CFBE cells expressing F508delCFTR,...
Source: American Journal of Respiratory Cell and Molecular Biology - October 7, 2020 Category: Molecular Biology Authors: Cabrita I, Benedetto R, Wanitchakool P, Lerias J, Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K Tags: Am J Respir Cell Mol Biol Source Type: research

Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation.
Abstract Sensitivity to allergenic fungi (Alternaria alternata) is associated with acute, severe asthma attacks. Antigen presenting cells (APCs) in the lung sense environmental perturbations that induce cellular stress and metabolic changes and are critical for allergic airway inflammation. However, the mechanisms underlying such environmental sensing by APCs in the lung remains unclear. Here we show that acute Alternaria challenge rapidly induces neutrophil accumulation in airways, and alter expressions of Pyruvate Kinase (PKM2) and hypoxia-inducible factor -1α (Hif-1α) that correlates with proinflammatory medi...
Source: Immunobiology - June 30, 2020 Category: Allergy & Immunology Authors: Jaiswal AK, Makhija S, Stahr N, Sandey M, Suryawanshi A, Mishra A Tags: Immunobiology Source Type: research