Human B cells infected by Trypanosoma cruzi undergo F-actin disruption and cell death via caspase-7 activation and cleavage of phospholipase C γ1.
Human B cells infected by Trypanosoma cruzi undergo F-actin disruption and cell death via caspase-7 activation and cleavage of phospholipase Cγ1. Immunobiology. 2020 Jan 16;:151904 Authors: Dos Santos MA, Alves Martins F, Borges BC, de Gouveia Santos J, Nascimento Alves R, Dias MH, BrígidoTavares PC, E Silva Brígido RT, Teixeira TL, Costa Rodrigues C, Cota Teixeira S, da Costa MS, da Silva AA, Barbosa Silva MJ, de Melo Rodrigues Ávila V, Patriarca Mineo TW, de Souza MA, Bahia D, da Silva CV Abstract B cells contribute to the immune system in many ways such as antigen present...
Source: Immunobiology - January 16, 2020 Category: Allergy & Immunology Authors: Dos Santos MA, Alves Martins F, Borges BC, de Gouveia Santos J, Nascimento Alves R, Dias MH, BrígidoTavares PC, E Silva Brígido RT, Teixeira TL, Costa Rodrigues C, Cota Teixeira S, da Costa MS, da Silva AA, Barbosa Silva MJ, de Melo Rodrigues Ávila V, Tags: Immunobiology Source Type: research

Innate immunity is a late event in the onset of gliadin-specific enteropathy in the HLA-DQ8 mice.
Abstract Celiac disease (CD) is a food enteropathy that occurs in genetically susceptible individuals following the ingestion of gluten. Both gluten cytotoxicity and immunity activation play a role in CD pathogenesis; however, the chronological assessment of the different pathogenic mechanisms remains elusive. The models developed so far have only partially addressed this issue. Herein, Ab°DQ8 transgenic mice were administered wheat gliadin and indomethacin for 10 days to induce enteropathy. Gliadin-induced alteration of the small intestinal architecture was associated with increased expression of tissue trans...
Source: Immunobiology - January 8, 2020 Category: Allergy & Immunology Authors: Maurano F, Ogita T, Luongo D, Rotondi Aufiero V, Bergamo P, Mazzarella G, Tanabe S, Rossi M Tags: Immunobiology Source Type: research

Insulin-like growth factor-1 enhances the expression of functional TSH receptor in orbital fibroblasts from thyroid-associated ophthalmopathy.
Abstract Thyroid-associated ophthalmopathy (TAO), an autoimmune disease, occurs in approximately 50 % of patients with Graves' hyperthyroidism. Thyroid-stimulating hormone receptor (TSHR) that is expressed in orbital fibroblasts is the autoimmune target in the development of TAO. In addition to thyroid-stimulating immunoglobulin (TSI), insulin-like growth factor (IGF)-1 is also involved in the development of TAO. IGF-1 has been reported to potentiate the effects of thyroid-stimulating hormone (TSH) and TSI on TSHR signaling. In the current study, we investigated the effects of IGF-1 on the cell surface expression ...
Source: Immunobiology - December 25, 2019 Category: Allergy & Immunology Authors: Paik JS, Kim SE, Kim JH, Lee JY, Yang SW, Lee SB Tags: Immunobiology Source Type: research

Positive association of Bx genotype, KIR2L5, KIR2DS5 and full-length KIR2DS4 with the risk of meningioma.
CONCLUSION: This case- control study suggests carriers of Bx genotype, KIR2DL5, KIR2DS5, 2DS4fl, ≥ 4 iKIR, CxTx subset as well as Cen AB and Tel AB are associated with an increased risk of developing meningioma whereas carrying KIR2DS4del, AA, C4TX genotypes and Cen AA, Tel AA, Tel BB reduce the genetic predisposition for meningioma. PMID: 31899050 [PubMed - as supplied by publisher] (Source: Immunobiology)
Source: Immunobiology - December 19, 2019 Category: Allergy & Immunology Authors: Barani S, Taghipour M, Ghaderi A Tags: Immunobiology Source Type: research

Neutrophil extracellular traps (NETs) formation induced by TGF- β in oral lichen planus - Possible implications for the development of oral cancer.
Neutrophil extracellular traps (NETs) formation induced by TGF-β in oral lichen planus - Possible implications for the development of oral cancer. Immunobiology. 2019 Dec 19;:151901 Authors: Jablonska E, Garley M, Surazynski A, Grubczak K, Iwaniuk A, Borys J, Moniuszko M, Ratajczak-Wrona W Abstract Oral Potentially Malignant Disorders (OPMDs) including Oral Lichen Planus (OLP) are associated with risk of transformation to oral squamous cell carcinoma (OSCC). Available data show that innate immune cells involving polymorphonuclear neutrophils (PMNs) with their ability to neutrophil extracellular t...
Source: Immunobiology - December 19, 2019 Category: Allergy & Immunology Authors: Jablonska E, Garley M, Surazynski A, Grubczak K, Iwaniuk A, Borys J, Moniuszko M, Ratajczak-Wrona W Tags: Immunobiology Source Type: research

Titration of Igs contained in an intravenous IgM-enriched preparation against selected pathogens involved in sepsis.
Abstract The goal of our work was to titer the IgG, IgM and IgA in Pentaglobin® (a preparation enriched in IgM), targeting specific surface antigens of Gram-positive and Gram-negative bacteria as well as a C. albicans strain. Lipopolysaccharides from Gram-negative bacteria, peptidoglycan and lipoteichoic acid from the other microorganisms were extracted and used in several ELISA assays in order to determine the titration of immunoglobulins in Pentaglobin® directed towards the aforementioned surface antigens. Our results showed an overall immunoglobulin titer of at least 103 in Pentaglobin® with some ex...
Source: Immunobiology - December 14, 2019 Category: Allergy & Immunology Authors: Stracquadanio S, Lo Verde F, Cialfi A, Zega A, Stefani S, Cafiso V Tags: Immunobiology Source Type: research

The oxidation state of cysteine thiols on the ectodomain of TLR2 and TLR4 influences intracellular signaling.
rd NP Abstract Signal transduction by the Toll-like receptors (TLRs) is a key component of innate immunity against many pathogens and also underlies a large burden of human diseases. Therefore, the mechanisms and regulation of signaling from the TLRs are of considerable interest. Here we seek to determine the molecular mechanism by which TLR2 and TLR4, members of the Toll-like receptor family, are activated by bacterial LPS, hyperoxia, and zymosan respectively. Our central hypothesis is that the oxidation state of cysteine thiols on the ectodomain of TLR2 and TLR4 are critical for pathogen-initiated intracellular ...
Source: Immunobiology - December 5, 2019 Category: Allergy & Immunology Authors: Zsengellér ZK, Gerard NP Tags: Immunobiology Source Type: research

Trypanosoma cruzi calreticulin: In vitro modulation of key immunogenic markers of both canine tumors and relevant immune competent cells.
Abstract Recombinant calreticulin from Trypanosoma cruzi (rTcCalr), the parasite responsible for Chagas' disease, binds to Canine Transmissible Venereal Tumor (CTVT) cells from primary cultures and to a canine mammary carcinoma cell line. A Complement-binding assay indicated that interaction of the first component C1q with these tumor cells operated independently of the rTcCalr-presence. This apparent independence could be explained by the important structural similarities that exist among rTcCarl, endogenous normal canine and/or mutated calreticulins present in several types of cancer. In phagocytosis assays, tum...
Source: Immunobiology - December 5, 2019 Category: Allergy & Immunology Authors: Cruz P, Sosoniuk-Roche E, Maldonado I, Torres CG, Ferreira A Tags: Immunobiology Source Type: research

Oral formulation angiotensin-(1-7) therapy attenuates pulmonary and systemics damage in mice with emphysema induced by elastase.
In this study, we evaluated the effect of treatment with Ang-(1-7) on pulmonary tissue damage and behavior of mice submitted to experimental model of elastase-induced pulmonary emphysema (PE). Initially, male C57BL/6 mice were randomly assigned into two main groups: control (CTRL) and PE. In the PE group, the animals received three intratracheal instillations of pancreatic porcine elastase (PPE) at 1-week intervals (0.2 IU in 50 μL of saline). The CTRL group received the same volume of saline solution (50 μL). Twenty-four hours after the last instillation, animals of the PE group were randomly divided into two groups...
Source: Immunobiology - December 4, 2019 Category: Allergy & Immunology Authors: Bastos AC, Magalhães GS, Gregório JF, Matos NA, Motta-Santos D, Bezerra FS, Santos RAS, Campagnole Santos MJ, Rodrigues-Machado MG Tags: Immunobiology Source Type: research

Research progresses of molecular mechanism of pyroptosis and its related diseases.
Abstract Pyroptosis is a newly discovered untypical form of programmed cell death by inflammatory response, which is dependent on the classic pathway of Caspase-1 and the non-canonical pathway of Caspase-11 in mice or orthologue Caspase-4/-5 in Humans. It has been found that the Gasdermin family of protein is a key molecule in the formation of membrane pores of pyroptosis. After being cleaved by inflammatory caspases, it releases a N-terminal fragment with perforating activity to trigger pyroptosis. That pyroptosis is closely related to the occurrence and development of certain diseases. Now, the molecular mechani...
Source: Immunobiology - December 3, 2019 Category: Allergy & Immunology Authors: Gong W, Shi Y, Ren J Tags: Immunobiology Source Type: research

Potential anti-arthritic and anti-inflammatory effects of TNF- α processing inhibitor-1 (TAPI-1): A new approach to the treatment of S. aureus arthritis.
This study aimed to unveil the potential anti-arthritic effects of TAPI-1 (TNF-α processing inhibitor-1), an inhibitor that inhibits TACE (TNF-α converting enzyme) mediated release of soluble TNF-α and its receptors along with attenuation of other inflammatory and joint destructive factors responsible for the progression of arthritis. Male Swiss albino mice were inoculated with live S. aureus (5 × 106 cells/mouse) for the development of septic arthritis. TAPI-1 was administered intraperitoneally (10 mg/kg body weight) post S. aureus infection at regular intervals. Throughout the...
Source: Immunobiology - November 30, 2019 Category: Allergy & Immunology Authors: Sultana S, Bishayi B Tags: Immunobiology Source Type: research

Regulation of expansion of CD11c+ B cells and anti-viral immunity by epithelial V-like antigen.
Abstract Prior work demonstrated that epithelial V-like antigen (EVA), a cell surface adhesion molecule, is expressed in B lymphocytes and is necessary for the efficacy of anti-alpha4 integrin treatment of experimental autoimmune encephalomyelitis (EAE), the mouse model of human multiple sclerosis. EVA deficiency is associated with a severe clinical phenotype of EAE in the presence or absence of treatment. Histological analysis revealed enhanced B cell-mediated autoimmunity and deposition of antibody and complement within the brain and spinal cord. Here our goal was to determine the molecular mechanism of EVA regu...
Source: Immunobiology - November 30, 2019 Category: Allergy & Immunology Authors: Dungan M, Carrithers MD Tags: Immunobiology Source Type: research

The analysis of organization and diversity mechanism in goat immunoglobulin light chain gene loci.
Abstract The genomic organization of goat immunoglobulin light chains (Igλ and Igκ) loci were annotated based on the goat genome database. The goat Igλ chain located on chromosome 17 contains at least 35 Vλ gene fragments (seven potential functional genes, one ORF and 27 pseudogenes), two Jλ-Cλ clusters arranged in a Vλ(35)-Jλ2-Cλ1-Jλ1-Cλ2 pattern, with another Cλ3 on scaffold. The Igκ locus included 11 Vκ (five potential functional genes, two ORFs and four pseudogene fragments), three Jκ genes and a single C&kappa...
Source: Immunobiology - November 30, 2019 Category: Allergy & Immunology Authors: Yu X, Du L, Wu M, Wu J, He S, Yuan T, Sun X Tags: Immunobiology Source Type: research

Macrophages from a type 1 diabetes mouse model present dysregulated Pl3K/AKT, ERK 1/2 and SAPK/JNK levels.
ns JO Abstract Diabetes causes dysregulation in signal transduction in immune cells leading to an impaired response to pathogens. Herein, we investigated the impact of type 1 diabetes (T1D) in bone marrow-derived macrophages (BMDM), using male non-diabetic and diabetic C57BL/6 mice (alloxan 60 mg/kg, i.v., CEUA/FCF/USP - 467). Diabetic BMDM expressed impaired phosphoinositide 3-kinase (PI3K), being lower p-PI3K p55 levels and higher levels of PI3K p110 alpha, whereas protein kinase B (PKB/Akt) (Ser-473 and Thr-308), extracellular signal-regulated kinases (ERK 1/2), and stress-activated protein kinase (SAPK/...
Source: Immunobiology - November 30, 2019 Category: Allergy & Immunology Authors: Galvão Tessaro FH, Ayala TS, Bella LM, Martins JO Tags: Immunobiology Source Type: research

Anti-inflammatory mediators ST2 and SIGIRR are induced by diphenyldifluoroketone EF24 in lipopolysaccharide-stimulated dendritic cells.
The objective of this study was to investigate the effect of EF24, an NF-κB-inhibitor, on the expression of negative regulators in IL-1R pathway, namely ST2 and SIGIRR. Murine JAWS II dendritic cells (DC) were challenged with lipopolysaccharide (LPS, 100 ng/ml) for 4 h, followed by treatment with 10 μM EF24 for 1 h. ST2 and SIGIRR expression was monitored by qRT-PCR and immunoblotting. ST2L and MyD88 interaction was studied by co-immunoprecipitation, and IL-33, a ST2L ligand, was assayed by ELISA. Activation of transcription factor SP1 was examined by confocal microscopy, immunoblotting...
Source: Immunobiology - November 29, 2019 Category: Allergy & Immunology Authors: Awasthi V, Vilekar P, Rao G, Awasthi S Tags: Immunobiology Source Type: research

Promising approaches in cancer immunotherapy.
Abstract Immunotherapy is a promising field, which enhances and harnesses the powers of the host immune system against cancer and in recent years, has become a major application of the fundamental research of cancer immunology. Cancer immunotherapy is often more targeted than non-specific therapy approaches, including radiotherapy or chemotherapy, as the immune system can be trained to remember cancer cells, highlighting a durable approach that can be maintained after the treatment completion. Immunotherapy functions by directing the immune system to attack the tumour cells via targeting tumour antigens, also enha...
Source: Immunobiology - November 29, 2019 Category: Allergy & Immunology Authors: Taefehshokr N, Baradaran B, Baghbanzadeh A, Taefehshokr S Tags: Immunobiology Source Type: research

HTLV infected individuals have increased B-cell activation and proinflammatory regulatory T-cells.
lash;nge BL Abstract Human T-lymphotropic virus (HTLV) affects the human immune system in many ways, most notably by inducing proliferation of infected CD4 + T cells, but several other cell types are also affected. To characterize the effects of HTLV infection, we analysed blood samples from HTLV-infected individuals by flow cytometry. Samples were collected from visitors at the HIV clinic in Bissau, Guinea-Bissau. These samples were tested for HTLV and HIV, and 199 were analysed by flow cytometry using panels for B cells, T-cell maturation and activation, regulatory T cells (Tregs) and monocytes. CD...
Source: Immunobiology - November 29, 2019 Category: Allergy & Immunology Authors: Kjerulff B, Petersen MS, Rodrigues CM, da Silva Té D, Christiansen M, Erikstrup C, Hønge BL Tags: Immunobiology Source Type: research

Regulation of hepcidin/iron-signalling pathway interactions by commensal bifidobateria plays an important role for the inhibition of metaflammation-related biomarkers.
CONCLUSION: Commensal bifidobacteria ameliorate metaflammation/inflammatory responses to excessive extracellular LPS, oleate and iron through at least two molecular/signaling mechanisms: i. modulation of interactions of the hepcidin- and iron-signaling pathways via reduction of excess iron; ii. reduction of pro-inflammatory cytokines and hepcidin production through inhibition of the TLR4/NF-kB pathway. This may be a molecular basis by which commensal bifidobacteria enhance intrinsic cellular tolerance against excess consumption of energy-yielding substrates and/or free iron. PMID: 31810825 [PubMed - as supplied by pub...
Source: Immunobiology - November 29, 2019 Category: Allergy & Immunology Authors: Ghadimi D, Yoness Hassan MF, Fölster-Holst R, Röcken C, Ebsen M, de Vrese M, Heller KJ Tags: Immunobiology Source Type: research

Precursor cells and implications of a T-cell inflamed immune response in the pre-malignant setting in Hodgkin lymphoma.
Abstract The etiology of classical Hodgkin lymphoma (cHL) is largely unknown. High serum CD30-levels are associated with increased risk of cHL. Epstein-Barr virus (EBV) is detectable in the tumor cells in 1/3 of cHL cases in the Western world. The PD-1 pathway (T-cell inflamed immune response) might contribute to the pathogenesis by enabling pre-malignant CD30+ or EBV + cells to evade immune surveillance. We aimed to investigate if high infiltrations of CD30+, PD-1+, PD-L1+ and EBV + cells in benign lymph nodes from patients that later develop cHL (cases) (n = 15) were associated with risk of cHL compared to contr...
Source: Immunobiology - November 25, 2019 Category: Allergy & Immunology Authors: Hollander P, Ginman B, Molin D, Enblad G, Amini RM, Glimelius I Tags: Immunobiology Source Type: research

Oxidative stress and immune complexes: Pathogenic mechanisms in pristane induced murine model of lupus.
This study investigated the effect of pristane administration on female BALB/c mice. The various manifestations of lupus replicated in this mouse model were analysed and their relevance to human disease was assessed. Pristane injection replicates the two prime manifestations of lupus i.e. oxidative stress and inflammation. An increase in oxidative stress was determined by the decreasing anti-oxidants, increasing ROS and lipid peroxidation. Inflammatory cytokines (IL-6 and TNF-α) also increased in the plasma. The deteriorating organ functions after pristane administration were evident histopathologically. An increase ...
Source: Immunobiology - November 19, 2019 Category: Allergy & Immunology Authors: Pannu N, Bhatnagar A Tags: Immunobiology Source Type: research

Three SNPs of FCRL3 and one SNP of MTMR3 are associated with immunoglobulin A nephropathy risk.
CONCLUSIONS: We suggest that three SNPs of FCRL3 were associated with a decreased risk of IgAN, while one SNP of MTMR3 was associated with an increased risk of IgAN in Chinese Han populations. These findings may be useful in the development of early prognostics for IgAN. PMID: 31780315 [PubMed - as supplied by publisher] (Source: Immunobiology)
Source: Immunobiology - November 18, 2019 Category: Allergy & Immunology Authors: Zhang H, He Y, He X, Wang L, Jin T, Yuan D Tags: Immunobiology Source Type: research

Neutrophil extracellular traps impair fungal clearance in a mouse model of invasive pulmonary aspergillosis.
Abstract Neutrophil extracellular traps (NETs) are formed by polymorphonuclear neutrophils (PMN) and contribute to the innate host defense by binding and killing bacterial and fungal pathogens. Because NET formation depends on histone hypercitrullination by peptidylarginine deiminase 4 (PAD4), we used PAD4 gene deficient (Pad4-/-) mice in a mouse model of invasive pulmonary aspergillosis (IPA) to address the contribution of NETs to the innate host defense in vivo. After the induction (24 h) of IPA by i.t. infection with Aspergillus fumigatus conidia, Pad4-/- mice revealed lower fungal burden in the lungs, a...
Source: Immunobiology - November 13, 2019 Category: Allergy & Immunology Authors: Alflen A, Aranda Lopez P, Hartmann AK, Maxeiner J, Bosmann M, Sharma A, Platten J, Ries F, Beckert H, Ruf W, Radsak MP Tags: Immunobiology Source Type: research

PGL-1 and LID-1 antibody levels in HIV-infected and HIV-uninfected individuals in a Hansen's disease (leprosy) endemic area of Brazil.
Abstract Serological tests for subclinical Mycobacterium leprae infection based on antibodies to phenolic glycolipid-1 (PGL-1) and leprosy IDRI diagnostic-1 (LID-1) have not been compared in HIV-infected and uninfected individuals. PGL-1 seropositivity by ELISA was 6.0 % (21/350) in HIV-infected compared with 29.1 % (102/350) in HIV-uninfected individuals (p 
Source: Immunobiology - November 10, 2019 Category: Allergy & Immunology Authors: Madureira BP, de Carvalho FM, Pessolani MC, Collin SM, Deps PD Tags: Immunobiology Source Type: research

Effect of laminin environments and tumor factors on the biology of myeloid dendritic cells.
The objective of the current studies was to investigate the angiogenic and immune profile of murine myeloid DCs upon interaction with laminin environments, with a particular emphasis on ovarian cancer. Our results show that murine ovarian tumors produce several types of laminins, as determined by PCR analysis, and also that tumor-associated DCs, both from ascites or solid tumors express adhesion molecules capable of interacting with these molecules as determined by flow cytometry and PCR analysis. Further, we established that DCs cultured on laminin upregulate both AKT and MEK signaling pathways, and that long-term culture...
Source: Immunobiology - November 10, 2019 Category: Allergy & Immunology Authors: Phillippi B, Singh M, Loftus T, Smith H, Muccioli M, Wright J, Pate M, Benencia F Tags: Immunobiology Source Type: research

The emerging role of neutrophils in neurodegeneration.
Abstract Neutrophils are the first line of defense in the innate immune system, helping to maintain tissue homeostasis as well as eliminating pathogens and self-components. The traditional view of neutrophils as simple phagocytes has been revised over the last decade as new research reveals their unappreciated complexity. Neutrophils are phenotypically and functionally heterogeneous, allowing them to act as modulators of both inflammation and immune responses. During acute inflammation, neutrophils perform a variety of beneficial effector functions, but when inflammation is induced by injury (sterile inflammation)...
Source: Immunobiology - November 9, 2019 Category: Allergy & Immunology Authors: Rossi B, Constantin G, Zenaro E Tags: Immunobiology Source Type: research

Structural bases that underline Trypanosoma cruzi calreticulin proinfective, antiangiogenic and antitumor properties.
jo JA, Ferreira A Abstract Microbes have developed mechanisms to resist the host immune defenses and some elicit antitumor immune responses. About 6 million people are infected with Trypanosoma cruzi, the protozoan agent of Chagas' disease, the sixth neglected tropical disease worldwide. Eighty years ago, G. Roskin and N. Klyuyeva proposed that T. cruzi infection mediates an anti-cancer activity. This observation has been reproduced by several other laboratories, but no molecular basis has been proposed. We have shown that the highly pleiotropic chaperone calreticulin (TcCalr, formerly known as TcCRT), translocate...
Source: Immunobiology - November 5, 2019 Category: Allergy & Immunology Authors: Peña Álvarez J, Teneb J, Maldonado I, Weinberger K, Rosas C, Lemus D, Valck C, Olivera-Nappa Á, Asenjo JA, Ferreira A Tags: Immunobiology Source Type: research

The interferon-induced helicase C domain-containing protein 1 gene variant (rs1990760) as an autoimmune-based pathology susceptibility factor.
CONCLUSIONS: There were significant associations of risk T allel of the analyzed polymorphism with all studied autoimmune diseases (GDOR = 1.34, p = 7.02e-03; MSOR = 1.36, p = 2.17e-02; LADA - OR = 3.36, p = 8.73e-07). We also found that the frequency of CT and TT genotypes of the rs1990760 IFIH1 gene only in females (with LADA, GD, MS) was significantly higher than those in the female control group (47%, 41% vs 44%, 34%; p = 1.32e-03, p = 4.39e-04; OR = 2.08, 95%CI: (1.33-3.28), OR = 2.29, 95% CI: (1.44-3.65) respectively). Our research has shown significant differences regarding so...
Source: Immunobiology - November 5, 2019 Category: Allergy & Immunology Authors: Wawrusiewicz-Kurylonek N, Gościk J, Chorąży M, Siewko K, Posmyk R, Zajkowska A, Citko A, Maciulewski R, Szelachowska M, Myśliwiec J, Jastrzębska I, Kułakowska A, Kochanowicz J, Krętowski AJ Tags: Immunobiology Source Type: research

CD163 overexpression using a macrophage-directed gene therapy approach improves wound healing in ex vivo and in vivo human skin models.
Sandoval EA Abstract Large tissue damage or wounds cause serious comorbidities and represent a major burden for patients, families, and health systems. Due to the pivotal role of immune cells in the proper resolution of inflammation and tissue repair, we focus our current study on the interaction of macrophages with skin cells, and specifically on the effects of CD163 gene induction in macrophages in wound healing. We hypothesize that the over-expression of the scavenger receptor gene CD163 in human macrophages would result in a more efficient wound healing process. Using 3D human wounded skin organotypic tissues,...
Source: Immunobiology - November 4, 2019 Category: Allergy & Immunology Authors: Ferreira DW, Ulecia-Morón C, Alvarado-Vázquez PA, Cunnane K, Moracho-Vilriales C, Grosick RL, Cunha TM, Romero-Sandoval EA Tags: Immunobiology Source Type: research

Mannose-binding lectin (MBL) in adult patients with inflammatory bowel disease.
Abstract Inflammatory bowel disease (IBD) refers to disorders associated with progressive inflammatory processes in the gastrointestinal system. IBD consists of two major forms, Crohn's disease (CD), and ulcerative colitis (UC). IBD became a global disease in the 21st century. Its pathogenesis is still not fully understood. Mannose-binding lectin (MBL) is a pattern-recognising molecule, involved in anti-microbial and anti-cancer immunity. It is able to opsonize microorganisms and abnormal host cells, and to initiate complement activation. The aim of this study was to investigate possible involvement of MBL in infl...
Source: Immunobiology - October 31, 2019 Category: Allergy & Immunology Authors: Bąk-Romaniszyn L, Świerzko AS, Sokołowska A, Durko Ł, Mierzwa G, Szala-Poździej A, Małecka-Panas E, Cedzyński M Tags: Immunobiology Source Type: research

Role of myeloid cells in the immunosuppressive microenvironment in gliomas.
Abstract Glioma is the most common primary brain cancer, and half of patients present a diagnosis of glioblastoma (GBM), its most aggressive and lethal form. Conventional therapies, including surgery, radiotherapy, and chemotherapy, have not resulted in major ameliorations in GBM survival outcome, which remains extremely poor. Recent immunotherapy improvements for other tumors, coupled with growing knowledge of the complex interactions between malignant glioma cells and the immune system, led to an exponential increase in glioma immunotherapy research. However, immunotherapeutic strategies in GBM have not yet reac...
Source: Immunobiology - October 19, 2019 Category: Allergy & Immunology Authors: Locarno CV, Simonelli M, Carenza C, Capucetti A, Stanzani E, Lorenzi E, Persico P, Della Bella S, Passoni L, Mavilio D, Bonecchi R, Locati M, Savino B Tags: Immunobiology Source Type: research

Response to: Bias in the use of a SSClow/CCR3pos gate to capture basophils in chronic urticaria?
Abstract A response to Chirumbolo S, Bjørklund G, and Vella A, Bias in the use of a SSClow/CCR3pos gate to capture basophils in chronic urticaria? Immunobiology, 224(3), 2019, 353-354. PMID: 31630898 [PubMed - as supplied by publisher] (Source: Immunobiology)
Source: Immunobiology - October 5, 2019 Category: Allergy & Immunology Authors: D'Auria E, De Amici M, Licari A, Caimmi S, Mantegazza C, Zuccotti GV, Marseglia GL Tags: Immunobiology Source Type: research

Corrigendum to "Benzanthrone induced immunotoxicity via oxidative stress and inflammatory mediators in Balb/c mice" [Immunobiology 220, (March) (2015) 369-381].
Corrigendum to "Benzanthrone induced immunotoxicity via oxidative stress and inflammatory mediators in Balb/c mice" [Immunobiology 220, (March) (2015) 369-381]. Immunobiology. 2019 Sep 20;: Authors: Tewari P, Roy R, Mishra S, Mandal P, Yadav A, Chaudhari BP, Chaturvedi RK, Dwivedi PD, Tripathi A, Das M PMID: 31548083 [PubMed - as supplied by publisher] (Source: Immunobiology)
Source: Immunobiology - September 20, 2019 Category: Allergy & Immunology Authors: Tewari P, Roy R, Mishra S, Mandal P, Yadav A, Chaudhari BP, Chaturvedi RK, Dwivedi PD, Tripathi A, Das M Tags: Immunobiology Source Type: research

PiggyBac-engineered T cells expressing a glypican-3-specific chimeric antigen receptor show potent activities against hepatocellular carcinoma.
Abstract Glypican-3 (GPC3) is an attractive target for chimeric antigen receptor (CAR)-T cell therapy, as it is overexpressed in most hepatocellular carcinoma (HCC) tissues but shows restricted expression in healthy adult tissues. Herein, we generated GPC3-specific CAR-T cells for HCC therapy by electroporation with plasmid DNA encoding the piggyBac (PB) transposon and the hyperactive piggyBac transposase simultaneously instead of by commonly-used viral vectors. Our results demonstrated that GPC3CAR gene was efficiently integrated into the genome of T cells utilizing the PB transposon system. Upon stimulation with...
Source: Immunobiology - September 8, 2019 Category: Allergy & Immunology Authors: Wang P, Qin W, Liu T, Jiang D, Cui L, Liu X, Fang Y, Tang X, Jin H, Qian Q Tags: Immunobiology Source Type: research

An immunoinformatics approach for design and validation of multi-subunit vaccine against Cryptosporidium parvum.
Abstract An immunoinformatics-based approach is explored for potential multi-subunit vaccine candidates against Cryptosporidium parvum. We performed protein structure based systematic methodology for the development of a proficient multi-subunit vaccine candidate against C. parvum based on their probability of antigenicity, allergenicity and transmembrane helices as the screening criteria. The best-screened epitopes like B-cell epitopes (BCL), Helper T-lymphocytes (HTL) and cytotoxic T- lymphocytes (CTL) were joined by using the appropriate linkers to intensify and develop the presentation and processing of the an...
Source: Immunobiology - September 6, 2019 Category: Allergy & Immunology Authors: Dhal AK, Pani A, Mahapatra RK, Yun SI Tags: Immunobiology Source Type: research

Diagnostic and prognostic roles of peripheral blood Toll-like receptor-4 and stanniocalcin-1 genes expression in acute lung injury.
This study aims to assess TLR-4 and SCT-1 genes expressions in peripheral blood of ALI patients. Total RNA was extracted from peripheral blood of 48 subjects (20 healthy controls, 28 ALI patients) and expressions of genes were assessed by real-Time qRT-PCR. The expression levels of TLR-4 and SCT-1 genes were significantly lower in ALI patients compared to controls (P 
Source: Immunobiology - September 4, 2019 Category: Allergy & Immunology Authors: Esawy MM, Shabana MA, Baioumy SA, Ismail NA Tags: Immunobiology Source Type: research

Inhibitor of γ-secretase alleviates middle ear inflammation by regulating Th2 response in OVA-mediated allergic OME in vivo.
In this study, we aimed to investigate the role of Notch signaling pathway in the ovalbumin (OVA)-mediated allergic OME in vivo. OVA-induced OME rats were treated with a control vehicle or a γ-secretase inhibitor, N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) suppressing the Notch signaling. We studied the effect of Notch signaling pathway in OME model, including histopathological assessment, the expression of Th1 cytokines (IFN-γ), Th2 cytokines (IL-4, IL-5), key transcription factors (T-bet, GATA-3) by quantitative real-time polymerase chain reaction (qRT-PCR). In addition, the ...
Source: Immunobiology - August 25, 2019 Category: Allergy & Immunology Authors: Zhao C, Li J, Yang J, Yang L, Chen P, Dou J, Zhao S Tags: Immunobiology Source Type: research

RAGE and CCR7 mediate the transmigration of Zika-infected monocytes through the blood-brain barrier.
In this study, we investigated whether the migration of ZIKV-infected MonoMac-1 (MM-1) cells through the BBB is dependent on chemokine receptor 7 (CCR7) and receptor for advanced glycation end (RAGE); we also determined whether high mobility group box protein 1 (HMGB1) could facilitate the permeabilization of endothelial cells. We demonstrated that ZIKV infects MM-1 cells, leading to milieu accumulation of HMGB1. Our results suggest that HMGB1 is involved in the dysregulation of primary human brain microvascular endothelial cell junction markers. Our results also indicate that the migration of ZIKV-infected monocytes is de...
Source: Immunobiology - August 25, 2019 Category: Allergy & Immunology Authors: de Carvalho GC, Borget MY, Bernier S, Garneau D, da Silva Duarte AJ, Dumais N Tags: Immunobiology Source Type: research

Contribution of Toll like receptor polymorphisms to dengue susceptibility and clinical outcome among eastern Indian patients.
Abstract Dengue infection has been one of the major public health concerns in India causing simple dengue fever (DF) to severe dengue infection. In the present study, contribution of TLR3, 7 and 8 polymorphisms towards dengue disease susceptibility and severity among Eastern Indian patients was analysed. Genomic DNA was extracted from blood of 201 dengue infected patients and 157 healthy individuals, followed by genotyping of eight polymorphisms of TLR3 (rs3775290), TLR7 (rs5741880, rs3853839, rs179008 and rs179010) and TLR8 (rs3764879, rs3764880 and rs5744080) genes by polymerase chain reaction-restriction fragme...
Source: Immunobiology - August 25, 2019 Category: Allergy & Immunology Authors: Mukherjee S, Tripathi A Tags: Immunobiology Source Type: research

NLRP3 inflammasome inhibitor glyburide expedites diabetic-induced impaired fracture healing.
Abstract Localized inflammation is accompanied by the diabetic-induced fracture. The present study aims to investigate the therapeutic effects of glyburide, an NLRP3 inflammasome inhibitor, in a diabetic-induced fracture model. An animal model of diabetic-induced fracture was established and the mice were administrated with metformin or glyburide for 3 weeks. Quantitative polymerase chain reaction (qPCR) and Western blotting were used to evaluate the relative expressions of IFN-γ, TNF-α, and IL-6. Micro-computed tomography (μCT) scanning was applied to evaluate bone callus formation. Histopathology ...
Source: Immunobiology - August 23, 2019 Category: Allergy & Immunology Authors: Yang X, Qu C, Jia J, Zhan Y Tags: Immunobiology Source Type: research

Vasoactive intestinal peptide alleviates food allergy via restoring regulatory B cell functions.
In conclusion, administration of VIP can alleviate FA response through up regulating expression of TSP1 to stabilize IL-10 expression in FA Br cells and recover the immune regulatory functions. The results have translational potential for the treatment of FA and other disorders associated with immune regulatory dysfunction of Br cells. PMID: 31471097 [PubMed - as supplied by publisher] (Source: Immunobiology)
Source: Immunobiology - August 23, 2019 Category: Allergy & Immunology Authors: Zeng HT, Zhao M, Yang SB, Huang H, Geng XR, Liu JQ, Yang G, Li DC, Yang LT, Zheng PY, Yang PC Tags: Immunobiology Source Type: research

Effects of intestinal nematode treatment on CD11b activation state in an EAE mouse model of multiple sclerosis.
Abstract The experimental autoimmune encephalomyelitis (EAE) animal model of Multiple Sclerosis (MS) is characterized by episodic neurologic dysfunction arising as a consequence of perivascular mononuclear cell infiltration and demyelination in the CNS. Leukocyte integrins, which are responsible for migration through the endothelial, play key roles in the pathogenesis of autoimmune diseases and chronic inflammation. Intestinal infection of mice with Heligmosomoides polygyrus appears to target CD11b (integrin αM), which is highly expressed on myeloid cells and is critical for their migration and function. H. ...
Source: Immunobiology - August 13, 2019 Category: Allergy & Immunology Authors: Donskow-Łysoniewska K, Krawczak K, Machcińska M, Głaczyńska M, Doligalska M Tags: Immunobiology Source Type: research

The prolactin inducible protein/gross cystic disease fluid protein-15 deficient mice develop anomalies in lymphoid organs.
Abstract The Prolactin Inducible Protein (PIP) is a 15 kDa protein secreted by normal apocrine glands, including salivary, lacrimal and sweat glands. PIP levels are normally low in the mammary glands of healthy individuals, but high levels have been observed in pathological conditions of the breast such as benign breast cystic disease and breast cancer. While the function of PIP is not well elucidated, accumulating evidence strongly point to a role in both innate and adaptive immunity. Using PIP deficient mice (Pip-/- mice) our laboratory demonstrated that loss of PIP function led to impaired T helper type 1 res...
Source: Immunobiology - August 13, 2019 Category: Allergy & Immunology Authors: Edechi CA, Nasr MR, Karim A, Blanchard AA, Ellison CA, Qui H, Uzonna JE, Myal Y Tags: Immunobiology Source Type: research

The covalent binding story of the complement proteins C3 and C4 (I) 1972-1981.
Abstract Alex Law and Paul Levine recall their work to establish the covalent bond between C3 and target surfaces. It started with a naive experiment by analyzing the membrane polypeptides of sheep erythrocytes bound with 125I-labelled C3. They found complexes with molecular weight higher than the individual C3 polypeptides. These complexes survived all conditions designed to disrupt non-covalent interactions. They then showed that the bond was an ester, with an active acyl group on C3 which reacted with a hydroxyl group on the acceptor molecule. With the discovery of an internal thioester by Jim Prahl, Jamila Jan...
Source: Immunobiology - August 13, 2019 Category: Allergy & Immunology Authors: Law SKA, Levine RP Tags: Immunobiology Source Type: research

Factors influencing multinucleated giant cell formation in vitro.
This study examined solutions to address these needs while providing context with other current and alternative methods. Primary mouse bone marrow-derived macrophages were treated with interleukin-4, a cytokine known to induce fusion into MGC. This model was used to systematically assess the influence of cell stimulant timing, cell seeding density, colony stimulating factors, and culture vessel type. Results indicated that MGC formation is greatly impacted by alterations in certain culture variables. An assessment of previously published research showed that these culture conditions varied widely between different laborato...
Source: Immunobiology - August 10, 2019 Category: Allergy & Immunology Authors: Trout KL, Holian A Tags: Immunobiology Source Type: research

TGF- β regulated leukemia cell susceptibility against NK targeting through the down-regulation of the CD48 expression.
In this study, we found that TGF-β induced leukemia MEG-01 and U937 cells to become less sensitive to NK-92MI targeting by down-regulating CD48, a ligand for NK activating receptor 2B4, but not down-regulating other tumor-associated carbohydrate antigens (TACAs). In CD48-knockdown cells, cells responding to NK-92MI targeting displayed a phenotype of less NK susceptibility and cell conjugation. On the other hand, when NK cells were treated with TGF-β, TGF-β suppressed NK recognition, degranulation, and killing activity in time-dependent manner by regulating ICAM-1 binding capacity instead of affecting express...
Source: Immunobiology - August 9, 2019 Category: Allergy & Immunology Authors: Huang CH, Liao YJ, Chiou TJ, Huang HT, Lin YH, Twu YC Tags: Immunobiology Source Type: research

Dexamethasone affects day/night development and function of thymus-derived T regulatory cells.
In this study we ask whether synthetic glucocorticoids commonly used to inhibit excessive activity of the immune system, can modulate the development and suppressive function of tTregs in vivo depending on the time of administration. Young C57BL/6 male and female mice were injected intraperitoneally with a single dose of dexamethasone at two time points of the day: 7.00-8.00 a.m. and 7.00-8.00 p.m. The experimental can be used to indicate on the potentially expected positive or adverse side effects and can constitute also a good model for the assessment of the effects of long-term therapy. The results of our studies demons...
Source: Immunobiology - August 7, 2019 Category: Allergy & Immunology Authors: Kiernozek E, Bieńkowska A, Markowska M, Kozlowska E, Drela N Tags: Immunobiology Source Type: research

Human TIGIT on porcine aortic endothelial cells suppresses xenogeneic macrophage-mediated cytotoxicity.
CONCLUSION: The findings of this study indicate that TIGIT suppresses xenogeneic M1 macrophage-induced cytotoxicity, probably at least in part, via the phosphorylation of SHP-1. In addition, the reduced expression of some pro-inflammatory cytokines, namely TNFα, IL-1β and IL-12, was observed in M1 macrophages that had been cultured with PAEC/TIGIT. PMID: 31402149 [PubMed - as supplied by publisher] (Source: Immunobiology)
Source: Immunobiology - August 3, 2019 Category: Allergy & Immunology Authors: Noguchi Y, Maeda A, Lo PC, Takakura C, Haneda T, Kodama T, Yoneyama T, Toyama C, Tazuke Y, Okuyama H, Miyagawa S Tags: Immunobiology Source Type: research

Dramatically decreased T cell responses but persistent IgE upon reduced pollen exposure.
Abstract Mugwort pollen allergy is frequent in parts of Europe. As mugwort pollen contains only one major allergen, Art v 1, which harbors only one T cell epitope, we employed mugwort pollen allergy as a model to study allergen-specific T cell responses. However, after 2004, we noticed a drastic decrease in the T cell responses to Art v 1 and eventually it became almost impossible to detect allergen-specific responses at the T cell level in mugwort-allergic individuals. To explain this observation, we retrospectively investigated the local exposure to mugwort pollen and its possible correlation to the frequency an...
Source: Immunobiology - August 3, 2019 Category: Allergy & Immunology Authors: Van Hemelen D, Hemmer W, Kmenta M, Berger UB, Kinaciyan T, Bohle B, Jahn-Schmid B Tags: Immunobiology Source Type: research

Immunoregulatory effects of very low density lipoprotein from healthy individuals and metabolic syndrome patients on glial cells.
In this study, VLDLs were isolated from normal healthy (nVLDL) individuals or patients with MetS (metVLDL). Primary astroglia and microglia mixed cell cultures as well as microglial-enriched cultures were used to test the effects of VLDLs. Microglia/astroglia activation as evidenced by their morphological changes and production of pro-inflammatory factors, such as tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2), were assessed by immunofluorescence staining and ELISA, respectively. Our results showed that metVLDLs mainly act on the microglia, and not the astroglia, with low concentration (0.05-0.5&th...
Source: Immunobiology - August 3, 2019 Category: Allergy & Immunology Authors: Li CL, Chu CH, Lee HC, Chou MC, Liu CK, Chen CH, Ke LY, Chen SL Tags: Immunobiology Source Type: research

IL12 and IFN γ secretion by donor mononuclear cells in response to host antigens may predict acute GVHD after HSCT.
IL12 and IFNγ secretion by donor mononuclear cells in response to host antigens may predict acute GVHD after HSCT. Immunobiology. 2019 Jul 20;: Authors: Kamel AM, Elsharkawy NM, Abdelfattah EK, Abdelfattah R, Samra MA, Wallace P, Mahmoud HK Abstract One persistent problem of allogeneic hematopoietic stem cell transplantation (HSCT) is acute graft versus host disease (GVHD). The role of cytokines in the pathogenesis of GVHD has been acknowledged. We aimed, in the current study, to investigate the possibility of prediction of acute GVHD through investigating the pattern of interleukin 12 (IL12) an...
Source: Immunobiology - July 20, 2019 Category: Allergy & Immunology Authors: Kamel AM, Elsharkawy NM, Abdelfattah EK, Abdelfattah R, Samra MA, Wallace P, Mahmoud HK Tags: Immunobiology Source Type: research