Deletion of mesencephalic astrocyte-derived neurotrophic factor delays and damages the development of white pulp in spleen
In this study, we detected MANF expression in spleen tissues and found that MANF was expressed in the red pulp and marginal zone. Additionally, MANF was localized in the CD68+ and CD138+ cells of adult rat spleen tissues, but not in the CD3+ cells. We performed immunohistochemical staining to detect MANF expression in the spleen tissues of rats that were different ages, and we found that MANF+ cells were localized together in the spleen tissues of rats that were 1-4 weeks old. MANF was also expressed in CD68+ cells in the spleen tissues of rats and mice. Furthermore, we found that MANF deficiency inhibited white pulp devel...
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Chengyue Zhou Dan Han Hui Fang Dake Huang Heping Cai Yujun Shen Yuxian Shen Jun Liu Source Type: research

Toll-like receptor expression and functional behavior in platelets from patients with systemic lupus erythematosus
CONCLUSION: Our study demonstrates normal platelet TLR expression and function together with increased circulating platelet-monocyte aggregates. In addition, a direct correlation was observed between plasma HMGB1 levels and platelet vWf release following TLR2 stimulation. This platelet behavior in a group of patients undergoing HCQ treatment suggests that platelets could play a role in the inflammatory state of SLE.PMID:38159527 | DOI:10.1016/j.imbio.2023.152782 (Source: Immunobiology)
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Mar ía C Baroni Pietto Ana C Glembotsky Paola R Lev Cecilia R Mar ín Oyarzún Geraldine De Luca Graciela Gomez Mar ía V Collado Nancy Char ó Adela S Cellucci Paula G Heller Nora P Goette Rosana F Marta Source Type: research

Engagement of CD300c by a novel monoclonal antibody induces the differentiation of monocytes to M1 macrophages
Immunobiology. 2023 Dec 21;229(1):152780. doi: 10.1016/j.imbio.2023.152780. Online ahead of print.ABSTRACTHuman CD300c is expressed on various immune or cancer cells and is a novel B7 family member, functioning as an activity modulator on immune cells. To elucidate the function of CD300c, we developed CL7, a human CD300c-specific monoclonal antibody, and assessed its biological activity. The specific binding of CL7 monoclonal antibody against recombinant CD300c antigen was confirmed using enzyme-linked immunosorbent assay and surface plasmon resonance analysis. The binding affinity of CL7 was strong at the sub-nanomolar le...
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Su In Lee Haneul Kim Chang Ki Lim Jae Dong Kim Jeong Seok Heo Joongoo Jung Chan Kim Hong Jae Chon Jae-Won Jeon Source Type: research

Deletion of mesencephalic astrocyte-derived neurotrophic factor delays and damages the development of white pulp in spleen
In this study, we detected MANF expression in spleen tissues and found that MANF was expressed in the red pulp and marginal zone. Additionally, MANF was localized in the CD68+ and CD138+ cells of adult rat spleen tissues, but not in the CD3+ cells. We performed immunohistochemical staining to detect MANF expression in the spleen tissues of rats that were different ages, and we found that MANF+ cells were localized together in the spleen tissues of rats that were 1-4 weeks old. MANF was also expressed in CD68+ cells in the spleen tissues of rats and mice. Furthermore, we found that MANF deficiency inhibited white pulp devel...
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Chengyue Zhou Dan Han Hui Fang Dake Huang Heping Cai Yujun Shen Yuxian Shen Jun Liu Source Type: research

Toll-like receptor expression and functional behavior in platelets from patients with systemic lupus erythematosus
CONCLUSION: Our study demonstrates normal platelet TLR expression and function together with increased circulating platelet-monocyte aggregates. In addition, a direct correlation was observed between plasma HMGB1 levels and platelet vWf release following TLR2 stimulation. This platelet behavior in a group of patients undergoing HCQ treatment suggests that platelets could play a role in the inflammatory state of SLE.PMID:38159527 | DOI:10.1016/j.imbio.2023.152782 (Source: Immunobiology)
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Mar ía C Baroni Pietto Ana C Glembotsky Paola R Lev Cecilia R Mar ín Oyarzún Geraldine De Luca Graciela Gomez Mar ía V Collado Nancy Char ó Adela S Cellucci Paula G Heller Nora P Goette Rosana F Marta Source Type: research

Engagement of CD300c by a novel monoclonal antibody induces the differentiation of monocytes to M1 macrophages
Immunobiology. 2023 Dec 21;229(1):152780. doi: 10.1016/j.imbio.2023.152780. Online ahead of print.ABSTRACTHuman CD300c is expressed on various immune or cancer cells and is a novel B7 family member, functioning as an activity modulator on immune cells. To elucidate the function of CD300c, we developed CL7, a human CD300c-specific monoclonal antibody, and assessed its biological activity. The specific binding of CL7 monoclonal antibody against recombinant CD300c antigen was confirmed using enzyme-linked immunosorbent assay and surface plasmon resonance analysis. The binding affinity of CL7 was strong at the sub-nanomolar le...
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Su In Lee Haneul Kim Chang Ki Lim Jae Dong Kim Jeong Seok Heo Joongoo Jung Chan Kim Hong Jae Chon Jae-Won Jeon Source Type: research

Deletion of mesencephalic astrocyte-derived neurotrophic factor delays and damages the development of white pulp in spleen
In this study, we detected MANF expression in spleen tissues and found that MANF was expressed in the red pulp and marginal zone. Additionally, MANF was localized in the CD68+ and CD138+ cells of adult rat spleen tissues, but not in the CD3+ cells. We performed immunohistochemical staining to detect MANF expression in the spleen tissues of rats that were different ages, and we found that MANF+ cells were localized together in the spleen tissues of rats that were 1-4 weeks old. MANF was also expressed in CD68+ cells in the spleen tissues of rats and mice. Furthermore, we found that MANF deficiency inhibited white pulp devel...
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Chengyue Zhou Dan Han Hui Fang Dake Huang Heping Cai Yujun Shen Yuxian Shen Jun Liu Source Type: research

Toll-like receptor expression and functional behavior in platelets from patients with systemic lupus erythematosus
CONCLUSION: Our study demonstrates normal platelet TLR expression and function together with increased circulating platelet-monocyte aggregates. In addition, a direct correlation was observed between plasma HMGB1 levels and platelet vWf release following TLR2 stimulation. This platelet behavior in a group of patients undergoing HCQ treatment suggests that platelets could play a role in the inflammatory state of SLE.PMID:38159527 | DOI:10.1016/j.imbio.2023.152782 (Source: Immunobiology)
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Mar ía C Baroni Pietto Ana C Glembotsky Paola R Lev Cecilia R Mar ín Oyarzún Geraldine De Luca Graciela Gomez Mar ía V Collado Nancy Char ó Adela S Cellucci Paula G Heller Nora P Goette Rosana F Marta Source Type: research

Engagement of CD300c by a novel monoclonal antibody induces the differentiation of monocytes to M1 macrophages
Immunobiology. 2023 Dec 21;229(1):152780. doi: 10.1016/j.imbio.2023.152780. Online ahead of print.ABSTRACTHuman CD300c is expressed on various immune or cancer cells and is a novel B7 family member, functioning as an activity modulator on immune cells. To elucidate the function of CD300c, we developed CL7, a human CD300c-specific monoclonal antibody, and assessed its biological activity. The specific binding of CL7 monoclonal antibody against recombinant CD300c antigen was confirmed using enzyme-linked immunosorbent assay and surface plasmon resonance analysis. The binding affinity of CL7 was strong at the sub-nanomolar le...
Source: Immunobiology - December 30, 2023 Category: Allergy & Immunology Authors: Su In Lee Haneul Kim Chang Ki Lim Jae Dong Kim Jeong Seok Heo Joongoo Jung Chan Kim Hong Jae Chon Jae-Won Jeon Source Type: research

Significance of m6A in subtype identification, immunological evolution, and therapeutic sensitivity of RA
In this study, we identified five differentially expressed m6A regulators in normal and RA samples from the GEO database. With these five regulators, we constructed the nomogram, and it could accurately identify the risk of RA morbidity. Next, we identified 121 differentially expressed genes (DEGs) between normal and RA samples, of which 36 DEGs were co-expressed with these five m6A regulators. We noted that these DEGs were highly enriched in multiple immunoregulatory signaling pathways, such as cytokine-mediated immune cell chemotaxis, adhesion, and activation. To further characterize the heterogeneity of immunological fe...
Source: Immunobiology - December 28, 2023 Category: Allergy & Immunology Authors: Chenxi Ma Jiasheng Wu Hongwei Lei He Huang Yingnan Li Source Type: research

Significance of m6A in subtype identification, immunological evolution, and therapeutic sensitivity of RA
In this study, we identified five differentially expressed m6A regulators in normal and RA samples from the GEO database. With these five regulators, we constructed the nomogram, and it could accurately identify the risk of RA morbidity. Next, we identified 121 differentially expressed genes (DEGs) between normal and RA samples, of which 36 DEGs were co-expressed with these five m6A regulators. We noted that these DEGs were highly enriched in multiple immunoregulatory signaling pathways, such as cytokine-mediated immune cell chemotaxis, adhesion, and activation. To further characterize the heterogeneity of immunological fe...
Source: Immunobiology - December 28, 2023 Category: Allergy & Immunology Authors: Chenxi Ma Jiasheng Wu Hongwei Lei He Huang Yingnan Li Source Type: research

Significance of m6A in subtype identification, immunological evolution, and therapeutic sensitivity of RA
In this study, we identified five differentially expressed m6A regulators in normal and RA samples from the GEO database. With these five regulators, we constructed the nomogram, and it could accurately identify the risk of RA morbidity. Next, we identified 121 differentially expressed genes (DEGs) between normal and RA samples, of which 36 DEGs were co-expressed with these five m6A regulators. We noted that these DEGs were highly enriched in multiple immunoregulatory signaling pathways, such as cytokine-mediated immune cell chemotaxis, adhesion, and activation. To further characterize the heterogeneity of immunological fe...
Source: Immunobiology - December 28, 2023 Category: Allergy & Immunology Authors: Chenxi Ma Jiasheng Wu Hongwei Lei He Huang Yingnan Li Source Type: research

Significance of m6A in subtype identification, immunological evolution, and therapeutic sensitivity of RA
In this study, we identified five differentially expressed m6A regulators in normal and RA samples from the GEO database. With these five regulators, we constructed the nomogram, and it could accurately identify the risk of RA morbidity. Next, we identified 121 differentially expressed genes (DEGs) between normal and RA samples, of which 36 DEGs were co-expressed with these five m6A regulators. We noted that these DEGs were highly enriched in multiple immunoregulatory signaling pathways, such as cytokine-mediated immune cell chemotaxis, adhesion, and activation. To further characterize the heterogeneity of immunological fe...
Source: Immunobiology - December 28, 2023 Category: Allergy & Immunology Authors: Chenxi Ma Jiasheng Wu Hongwei Lei He Huang Yingnan Li Source Type: research

Levels of soluble TNF receptors (sTNFR1 and sTNFR2) increase with clinical worsening of patients and are related to COVID-19 mortality
Immunobiology. 2023 Sep 15;229(1):152748. doi: 10.1016/j.imbio.2023.152748. Online ahead of print.ABSTRACTThe present study aimed to inspect the serum levels of the soluble receptors, sTNFR1 and sTNFR2, in patients with COVID-19. The large production of inflammatory cytokines is an essential process in the pathogenesis of COVID-19. TNF is a multifaceted proinflammatory cytokine which has soluble and membrane receptors. Thus, knowing the role of these receptors will help better understand this disease's immunopathogenesis. We included 131 patients confirmed for SARS-CoV-2, separated into three groups: ward patients without ...
Source: Immunobiology - December 21, 2023 Category: Allergy & Immunology Authors: Melayne Rocha Aciole Juliana Prado Gon çales Patr ícia Areias Feitosa Neves Cynthia Regina Pedrosa Soares Marta Iglis de Oliveira Heloisa Ramos Lacerda de Melo Reginaldo Gon çalves de Lima Neto L íbia Cristina Rocha Vilela Moura Paulo Sergio Ramos Ara Source Type: research

Levels of soluble TNF receptors (sTNFR1 and sTNFR2) increase with clinical worsening of patients and are related to COVID-19 mortality
Immunobiology. 2023 Sep 15;229(1):152748. doi: 10.1016/j.imbio.2023.152748. Online ahead of print.ABSTRACTThe present study aimed to inspect the serum levels of the soluble receptors, sTNFR1 and sTNFR2, in patients with COVID-19. The large production of inflammatory cytokines is an essential process in the pathogenesis of COVID-19. TNF is a multifaceted proinflammatory cytokine which has soluble and membrane receptors. Thus, knowing the role of these receptors will help better understand this disease's immunopathogenesis. We included 131 patients confirmed for SARS-CoV-2, separated into three groups: ward patients without ...
Source: Immunobiology - December 21, 2023 Category: Allergy & Immunology Authors: Melayne Rocha Aciole Juliana Prado Gon çales Patr ícia Areias Feitosa Neves Cynthia Regina Pedrosa Soares Marta Iglis de Oliveira Heloisa Ramos Lacerda de Melo Reginaldo Gon çalves de Lima Neto L íbia Cristina Rocha Vilela Moura Paulo Sergio Ramos Ara Source Type: research