Phenolic composition of rooibos changes during simulated fermentation: Effect of endogenous enzymes and fermentation temperature on reaction kinetics
Publication date: Available online 19 March 2019Source: Food Research InternationalAuthor(s): Dalene De Beer, Jade Tobin, Beata Walczak, Marieta Van Der Rijst, Elizabeth JoubertAbstractPhenolic compounds of Aspalathus linearis (rooibos) are susceptible to oxidation during “fermentation”, a process characterized by the formation of a red-brown leaf color. The role of enzymes in this process is not yet understood. An experiment with dried green rooibos plant material pre-treated at 170 °C for 30 min to denature and “inactivate” endogenous enzymes was conducted to confirm the role of oxidative enzymes. The phenol...
Source: Food Research International - March 19, 2019 Category: Food Science Source Type: research

Molecules, Vol. 24, Pages 938: Visualization of Aspalathin in Rooibos (Aspalathus linearis) Plant and Herbal Tea Extracts Using Thin-Layer Chromatography
Uljana Hesse Aspalathin, the main polyphenol of rooibos (Aspalathus linearis), is associated with diverse health promoting properties of the tea. During fermentation, aspalathin is oxidized and concentrations are significantly reduced. Standardized methods for quality control of rooibos products do not investigate aspalathin, since current techniques of aspalathin detection require expensive equipment and expertise. Here, we describe a simple and fast thin-layer chromatography (TLC) method that can reproducibly visualize aspalathin in rooibos herbal tea and plant extracts at a limit of detection (LOD) equal to 178.7 n...
Source: Molecules - March 6, 2019 Category: Chemistry Authors: Emily Amor Stander Wesley Williams Fanie Rautenbach Marilize Le Roes-Hill Yamkela Mgwatyu Jeanine Marnewick Uljana Hesse Tags: Article Source Type: research

Rooibos (Aspalathus linearis) extract, containing eriodictyol-6-C-β-d-glucoside as the active component, stimulates exocrine glands via the M3 muscarinic acetylcholine receptor
In conclusion, rooibos extract contains E6CG as a M3R activator and stimulates exocrine gland secretion, which relieves dryness at the mouth, eyes, and skin in humans.Graphical abstract (Source: Journal of Functional Foods)
Source: Journal of Functional Foods - February 26, 2019 Category: Nutrition Source Type: research

The effects of rooibos (Aspalathus linearis) on 3T3-L1 preadipocytes after the induction of mitochondrial dysfunction
Publication date: April 2019Source: Journal of Functional Foods, Volume 55Author(s): Anna C. Hattingh, Maryna van de Venter, Trevor C. KoekemoerAbstractRooibos (Aspalathus linearis) is a South African fynbos plant, well-known for its strong anti-oxidant capacity and use in cosmetic products. The anti-ageing properties of fermented and green (unfermented) rooibos were investigated using a cell culture model designed to evaluate the involvement of mitochondrial dysfunction in the age-related decline in preadipocyte function. Mitochondrial dysfunction was induced through long-term exposure of 3T3-L1 preadipocytes to ethidium ...
Source: Journal of Functional Foods - February 24, 2019 Category: Nutrition Source Type: research

Letter to the Editor on Cistus incanus a promising herbal tea rich in bioactive compounds: LC–MS/MS determination of catechins, flavonols, phenolic acids and alkaloids—A comparison with Camellia sinensis, Rooibos and Hoan Ngoc herbal tea
Publication date: Available online 2 February 2019Source: Journal of Food Composition and AnalysisAuthor(s): Dalene de Beer (Source: Journal of Food Composition and Analysis)
Source: Journal of Food Composition and Analysis - February 3, 2019 Category: Food Science Source Type: research

Response to Letter to the Editor on Cistus incanus a promising herbal tea rich in bioactive compounds: LC–MS/MS determination of catechins, flavonols, phenolic acids and alkaloids—A comparison with Camellia sinensis, Rooibos and Hoan Ngoc herbal tea
Publication date: Available online 2 February 2019Source: Journal of Food Composition and AnalysisAuthor(s): Magdalena Jeszka-Skowron, Agnieszka Zgoła-Grześkowiak, Robert Frankowski (Source: Journal of Food Composition and Analysis)
Source: Journal of Food Composition and Analysis - February 3, 2019 Category: Food Science Source Type: research

Rooibos tea (Aspalathus linearis) ameliorates the CCl4-induced injury to mitochondrial respiratory function and energy production in rat liver.
Abstract The rooibos tea (RT) is a source of valuable dietary dihydrochalcones  aspalathin, and nothofagin and other polyphenols. Many in vitro and in vivo studies have shown that RT flavonoids have strong antioxidant effect and significantly reduce oxidative stress. We investigated the antioxidant activity and protective effect of an aqueous extract of RT on the liver mitochondria oxidative phosphorylation in rats with carbon tetrachloride-induced (CCl4-induced) liver damage. Mitochondrial respiration and ATP production was determined amperometrically using a Clark-type oxygen electrode. We found signi...
Source: General Physiology and Biophysics - January 18, 2019 Category: Physiology Authors: Uličná O, Vančová O, Kucharská J, Janega P, Waczulíková I Tags: Gen Physiol Biophys Source Type: research

Revisiting the caffeine-free status of rooibos and honeybush herbal teas using specific MRM and high resolution LC-MS methods
Publication date: Available online 7 December 2018Source: Journal of Food Composition and AnalysisAuthor(s): Maria A. Stander, Elizabeth Joubert, Dalene De BeerAbstractTwo endemic South African herbal teas, namely rooibos (Aspalathus linearis) and honeybush (Cyclopia spp.), are especially valued for their caffeine-free status. No recent evidence based on state-of-the-art analytical methodology with low limits of detection (e.g. below 1 μg/L) is available. The current study provides such evidence. Randomly selected samples of fermented rooibos (n = 10) and honeybush herbal teas from four species (n = 10 each), na...
Source: Journal of Food Composition and Analysis - December 8, 2018 Category: Food Science Source Type: research

Molecules, Vol. 23, Pages 3207: The Potential of South African Herbal Tisanes, Rooibos and Honeybush in the Management of Type 2 Diabetes Mellitus
olaju Diabetes mellitus is a metabolic disease that can lead to high morbidity, mortality and long-term complications. Available treatment strategies, which are mainly based on treating hyperglycemia, with insulin and other pharmacological agents are not completely efficient and can even lead to development of unwanted side effects. Scientific evidence suggests that bioactive compounds from teas and other plant-based foods, which are known source of natural antioxidants, could be an attractive strategy to preferentially treat and manage type 2 diabetes mellitus (T2DM) and thus, have significant therapeutic implications...
Source: Molecules - December 5, 2018 Category: Chemistry Authors: Olawale R. Ajuwon Ademola O. Ayeleso Gbenga A. Adefolaju Tags: Review Source Type: research

Renal protective effects of aspalathin and nothofagin from rooibos (Aspalathus linearis) in a mouse model of sepsis.
CONCLUSION: Our results suggest that Aspt and Not protect mice against sepsis-triggered renal injury. PMID: 30340097 [PubMed - indexed for MEDLINE] (Source: Pharmacological Reports)
Source: Pharmacological Reports - December 1, 2018 Category: Drugs & Pharmacology Authors: Yang S, Lee C, Lee BS, Park EK, Kim KM, Bae JS Tags: Pharmacol Rep Source Type: research

The commercial history of Cape herbal teas and the analysis of phenolic compounds in historic teas from a depository of 1933
Publication date: Available online 13 November 2018Source: Journal of Food Composition and AnalysisAuthor(s): M.A. Stander, T. Brendler, H. Redelinghuys, B.-E. Van WykAbstractIn an investigation into the historical use of various plant species as herbal teas in South Africa and the commercialization of rooibos (Aspalathus linearis), 37 tea samples from a depository of 1933 were analysed. These samples are known as the Nortier collection and were put in the depository by Benjamin Ginsberg. Nortier and Ginsberg were instrumental in the commercialisation of Rooibos tea. Liquid-chromatography–high resolution mass spectrometr...
Source: Journal of Food Composition and Analysis - November 14, 2018 Category: Food Science Source Type: research

Cistus incanus a promising herbal tea rich in bioactive compounds: LC–MS/MS determination of catechins, flavonols, phenolic acids and alkaloids—A comparison with Camellia sinensis, Rooibos and Hoan Ngoc herbal tea
In this study the content of 28 phenolic compounds and theirs derivatives, alkaloids and vitamin B of water extract of Cistus incanus tea was examined and the results were compared with the results from other types of popular in the market teas. The Cistus incanus infusions were tested for content of flavanols, flavonols, organic acids, vitamin B and alkaloids and were compared with Camellia sinensis, Hoan Ngoc herbal tea and Rooibos infusions. Camellia sinensis infusions generally contained more catechins (1.56–82.65 mg/g) than Cistus incanus (1.02–2.73 mg/g) but there was no catechin-3-gallate in any Camellia sin...
Source: Journal of Food Composition and Analysis - September 20, 2018 Category: Food Science Source Type: research

Cistus incanus a promising herbal tea rich in bioactive compounds: LC-MS/MS determination of catechins, flavonols, phenolic acids and alkaloids – A comparison with Camellia sinensis, Rooibos and Hoan Ngoc herbal tea
In this study the content of 28 phenolic compounds and theirs derivatives of water extract of Cistus incanus tea was examined and the results were compared with the results from other types of popular in the market teas. The Cistus incanus infusions were tested for content of flavanols, flavonols, organic acids, vitamin B and alkaloids and were compared with Camellia sinensis, Hoan Ngoc herbal tea and Rooibos infusions. Camellia sinensis infusions generally contained more catechins (1.56-82.65 mg/g) than Cistus incanus (1.02-2.73 mg/g) but there was no catechin-3-gallate in any Camellia sinensis infusions. Caffeine, th...
Source: Journal of Food Composition and Analysis - September 13, 2018 Category: Food Science Source Type: research

Aspalathin, a C-glucosyl dihydrochalcone from rooibos improves the hypoglycemic potential of metformin in type 2 diabetic (db/db) mice.
This study reports on the combinational use of metformin with aspalathin, a C-glucosyl dihydrochalcone with known glucose lowering and antioxidant properties, as an effective hypoglycemic therapy in a type 2 diabetic (db/db) mouse model. When tested as a monotherapy, a low dose of aspalathin (13 mg/kg) showed no effect, while a high dose (130 mg/kg) has already displayed a better potential than metformin in protecting against diabetes associated symptoms in db/db mice. Thus, it remains of interest to determine whether this dihydrochalcone can improve the efficacy of metformin. The results showed that this combination thera...
Source: Physiological Research - July 25, 2018 Category: Physiology Authors: Dludla PV, Gabuza KB, Muller CJF, Joubert E, Louw J, Johnson R Tags: Physiol Res Source Type: research

Inulin as microencapsulating agent improves physicochemical properties of spray-dried aspalathin-rich green rooibos (Aspalathus linearis) extract with α-glucosidase inhibitory activity
Publication date: September 2018Source: Journal of Functional Foods, Volume 48Author(s): Neil Miller, Dalene De Beer, Marique Aucamp, Christiaan J. Malherbe, Elizabeth JoubertAbstractLimiting aspalathin degradation during spray-drying of green rooibos extract (GRE) is crucial to produce a functional food ingredient with antidiabetic properties. Retention of aspalathin in a GRE-inulin mixture (1:1, m/m) was not affected by process conditions (inlet air temperature, feed concentration and feed flow rate) tested according to a central composite design. Powder yield could subsequently be optimised by applying response surface ...
Source: Journal of Functional Foods - July 21, 2018 Category: Nutrition Source Type: research