Resprouters Versus Reseeders: Are Wild Rooibos Ecotypes Genetically Distinct?
This study aimed to test the hypothesis that wild rooibos ecotypes are distinct in genetic variability and that the ecotypes found in the Northern Cape are differentiated from those in the Cederberg that may be linked to a fire-survival strategy as well as distinct morphological and phytochemical differences. A phylogeographical and population genetic analyses of both chloroplast (trnLF intergenic region) and newly developed species-specific nuclear markers (microsatellites) was performed on six geographically representative wild rooibos populations. From the diversity indices, it was evident that the wild rooibos populati...
Source: Frontiers in Genetics - December 20, 2021 Category: Genetics & Stem Cells Source Type: research

The toxic contaminants of Aspalathus linearis plant material as well as herb –drug interactions may constitute the health risk factors in daily rooibos tea consumers
. (Source: International Journal of Environmental Health Research)
Source: International Journal of Environmental Health Research - November 26, 2021 Category: Environmental Health Authors: Justyna Pyrzanowska Source Type: research

Molecules, Vol. 26, Pages 6289: Rooibos Flavonoids, Aspalathin, Isoorientin, and Orientin Ameliorate Antimycin A-Induced Mitochondrial Dysfunction by Improving Mitochondrial Bioenergetics in Cultured Skeletal Muscle Cells
ibuko-Mbeje The current study investigated the physiological effects of flavonoids found in daily consumed rooibos tea, aspalathin, isoorientin, and orientin on improving processes involved in mitochondrial function in C2C12 myotubes. To achieve this, C2C12 myotubes were exposed to a mitochondrial channel blocker, antimycin A (6.25 µM), for 12 h to induce mitochondrial dysfunction. Thereafter, cells were treated with aspalathin, isoorientin, and orientin (10 µM) for 4 h, while metformin (1 µM) and insulin (1 µM) were used as comparators. Relevant bioassays and real-time PCR were conducted to assess the impact of tr...
Source: Molecules - October 18, 2021 Category: Chemistry Authors: Sinenhlanhla X. H. Mthembu Christo J. F. Muller Phiwayinkosi V. Dludla Evelyn Madoroba Abidemi P. Kappo Sithandiwe E. Mazibuko-Mbeje Tags: Article Source Type: research

Molecules, Vol. 26, Pages 6154: Orientin Improves Substrate Utilization and the Expression of Major Genes Involved in Insulin Signaling and Energy Regulation in Cultured Insulin-Resistant Liver Cells
nkosi V. Dludla Our group has progressively reported on the impact of bioactive compounds found in rooibos (Aspalathus linearis) and their capacity to modulate glucose homeostasis to improve metabolic function in experimental models of type 2 diabetes. In the current study, we investigated how the dietary flavone, orientin, modulates the essential genes involved in energy regulation to enhance substrate metabolism. We used a well-established hepatic insulin resistance model of exposing C3A liver cells to a high concentration of palmitate (0.75 mM) for 16 hrs. These insulin-resistant liver cells were treated with orient...
Source: Molecules - October 12, 2021 Category: Chemistry Authors: Sithandiwe E. Mazibuko-Mbeje Sinenhlanhla X.H. Mthembu Andani Tshiitamune Ndivhuwo Muvhulawa Fikile T. Mthiyane Khanyisani Ziqubu Christo J.F. Muller Phiwayinkosi V. Dludla Tags: Communication Source Type: research

Organic-matter decomposition as a bioassessment tool of stream functioning: A comparison of eight decomposition-based indicators exposed to different environmental changes
Environ Pollut. 2021 Sep 4;290:118111. doi: 10.1016/j.envpol.2021.118111. Online ahead of print.ABSTRACTOrganic-matter decomposition has long been proposed as a tool to assess stream functional integrity, but this indicator largely depends on organic-matter selection. We assessed eight decomposition-based indicators along two well-known environmental gradients, a nutrient-enrichment gradient (0.2-1.4 mg DIN/L) in central Portugal and an acidification gradient (pH: 4.69-7.33) in north-eastern France to identify the most effective organic-matter indicator for assessing stream functional integrity. Functional indicators inclu...
Source: Environmental Pollution - September 15, 2021 Category: Environmental Health Authors: Ver ónica Ferreira Jo ão Silva Julien Cornut Ol ímpia Sobral Quentin Bachelet Jonathan Bouquerel Michael Danger Source Type: research

Organic-matter decomposition as a bioassessment tool of stream functioning: A comparison of eight decomposition-based indicators exposed to different environmental changes
Environ Pollut. 2021 Sep 4;290:118111. doi: 10.1016/j.envpol.2021.118111. Online ahead of print.ABSTRACTOrganic-matter decomposition has long been proposed as a tool to assess stream functional integrity, but this indicator largely depends on organic-matter selection. We assessed eight decomposition-based indicators along two well-known environmental gradients, a nutrient-enrichment gradient (0.2-1.4 mg DIN/L) in central Portugal and an acidification gradient (pH: 4.69-7.33) in north-eastern France to identify the most effective organic-matter indicator for assessing stream functional integrity. Functional indicators inclu...
Source: Environmental Pollution - September 15, 2021 Category: Environmental Health Authors: Ver ónica Ferreira Jo ão Silva Julien Cornut Ol ímpia Sobral Quentin Bachelet Jonathan Bouquerel Michael Danger Source Type: research

Organic-matter decomposition as a bioassessment tool of stream functioning: A comparison of eight decomposition-based indicators exposed to different environmental changes
Environ Pollut. 2021 Sep 4;290:118111. doi: 10.1016/j.envpol.2021.118111. Online ahead of print.ABSTRACTOrganic-matter decomposition has long been proposed as a tool to assess stream functional integrity, but this indicator largely depends on organic-matter selection. We assessed eight decomposition-based indicators along two well-known environmental gradients, a nutrient-enrichment gradient (0.2-1.4 mg DIN/L) in central Portugal and an acidification gradient (pH: 4.69-7.33) in north-eastern France to identify the most effective organic-matter indicator for assessing stream functional integrity. Functional indicators inclu...
Source: Environmental Pollution - September 15, 2021 Category: Environmental Health Authors: Ver ónica Ferreira Jo ão Silva Julien Cornut Ol ímpia Sobral Quentin Bachelet Jonathan Bouquerel Michael Danger Source Type: research

Organic-matter decomposition as a bioassessment tool of stream functioning: A comparison of eight decomposition-based indicators exposed to different environmental changes
Environ Pollut. 2021 Sep 4;290:118111. doi: 10.1016/j.envpol.2021.118111. Online ahead of print.ABSTRACTOrganic-matter decomposition has long been proposed as a tool to assess stream functional integrity, but this indicator largely depends on organic-matter selection. We assessed eight decomposition-based indicators along two well-known environmental gradients, a nutrient-enrichment gradient (0.2-1.4 mg DIN/L) in central Portugal and an acidification gradient (pH: 4.69-7.33) in north-eastern France to identify the most effective organic-matter indicator for assessing stream functional integrity. Functional indicators inclu...
Source: Environmental Pollution - September 15, 2021 Category: Environmental Health Authors: Ver ónica Ferreira Jo ão Silva Julien Cornut Ol ímpia Sobral Quentin Bachelet Jonathan Bouquerel Michael Danger Source Type: research

Molecules, Vol. 26, Pages 5260: Shelf-Life Stability of Ready-to-Use Green Rooibos Iced Tea Powder —Assessment of Physical, Chemical, and Sensory Properties
Molecules, Vol. 26, Pages 5260: Shelf-Life Stability of Ready-to-Use Green Rooibos Iced Tea Powder—Assessment of Physical, Chemical, and Sensory Properties Molecules doi: 10.3390/molecules26175260 Authors: Chantelle Human Dalene de Beer Magdalena Muller Marieta van der Rijst Marique Aucamp Andreas Tredoux André de Villiers Elizabeth Joubert Green rooibos extract (GRE), shown to improve hyperglycemia and HDL/LDL blood cholesterol, has potential as a nutraceutical beverage ingredient. The main bioactive compound of the extract is aspalathin, a C-glucosyl dihydrochalcone. The study aimed to determine ...
Source: Molecules - August 30, 2021 Category: Chemistry Authors: Chantelle Human Dalene de Beer Magdalena Muller Marieta van der Rijst Marique Aucamp Andreas Tredoux Andr é de Villiers Elizabeth Joubert Tags: Article Source Type: research

Rooibos (Aspalathus linearis) influence on health and ovarian functions
AbstractThis paper reviews provenance, processing and properties of rooibos (Aspalathus linearis, Brum.f) and its numerous biologically active constituents, as well as the currently available knowledge concerning their physiological and medicinal effects and their possible extra- and intracellular mechanisms of action. Search for literature was performed in agreement with the preferred reporting items for systematic review criteria in Cochrane Library, PubMed, Web of Science and SCOPUS databases between the years 2000 and 2021. The limited number of in vitro studies suggests an influence of rooibos on basic ovarian cell fu...
Source: Journal of Animal Physiology and Animal Nutrition - August 17, 2021 Category: Zoology Authors: Alexander V. Sirotkin Tags: REVIEW ARTICLE Source Type: research

Risk assessment of heavy metals in rooibos (Aspalathus linearis) tea consumed in South Africa
Environ Sci Pollut Res Int. 2021 Jun 18. doi: 10.1007/s11356-021-14968-2. Online ahead of print.ABSTRACTA total of 80 rooibos tea samples from a range of brands were purchased from various registered retail shops in South Africa. The samples were bought during 2019 winter (40) and summer (40) period which are classified as 6 natural rooibos, 18 herbal rooibos samples, and 16 flavor rooibos samples and subjected for heavy metal analysis such as chromium (Cr), iron (Fe), arsenic (As), cadmium (Cd), and lead (Pb) using inductively coupled plasma mass spectrometer (ICP-MS). Human health risks were determined by estimating the ...
Source: Environmental Science and Pollution Research International - June 19, 2021 Category: Environmental Health Authors: Oluwaseun Mary Areo Patrick Berka Njobeh Source Type: research

Risk assessment of heavy metals in rooibos (Aspalathus linearis) tea consumed in South Africa
Environ Sci Pollut Res Int. 2021 Jun 18. doi: 10.1007/s11356-021-14968-2. Online ahead of print.ABSTRACTA total of 80 rooibos tea samples from a range of brands were purchased from various registered retail shops in South Africa. The samples were bought during 2019 winter (40) and summer (40) period which are classified as 6 natural rooibos, 18 herbal rooibos samples, and 16 flavor rooibos samples and subjected for heavy metal analysis such as chromium (Cr), iron (Fe), arsenic (As), cadmium (Cd), and lead (Pb) using inductively coupled plasma mass spectrometer (ICP-MS). Human health risks were determined by estimating the ...
Source: Environmental Science and Pollution Research International - June 19, 2021 Category: Environmental Health Authors: Oluwaseun Mary Areo Patrick Berka Njobeh Source Type: research

The Effect of Rooibos (Aspalathus linearis), Honeybush (Cyclopia intermedia) and Sutherlandia (Lessertia frutescens) on Testicular Insulin Signalling in Streptozotocin-Induced Diabetes in Wistar Rats
(Source: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy)
Source: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy - March 18, 2021 Category: Endocrinology Tags: Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy Source Type: research