Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280. doi: 10.33549/physiolres.935060.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunoc...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Rooibos (Aspalathus linearis) and its constituent quercetin can suppress ovarian cell functions and their response to FSH
Physiol Res. 2023 Apr 30;72(2):269-280.ABSTRACTRooibos (Aspalathus linearis Brum. f) can directly influence female reproduction, but whether rooibos can influence the response of ovarian cells to FSH and whether the rooibos effects are due to the presence of quercetin remain unknown. We compared the influence of rooibos extract and quercetin (both at 10 µg/ml-1) on porcine ovarian granulosa cells cultured with and without FSH (0, 1, 10 or 100 ng/ml-1). The expression of intracellular proliferation (PCNA, cyclin B1) and apoptosis (bax, caspase 3) markers in the cells was detected by immunocytochemistry. The release of prog...
Source: Physiological Research - May 9, 2023 Category: Physiology Authors: A V Sirotkin Z Fabova W Aldahmash A Alshamrani A H Harrath Source Type: research
Solar radiation explains litter degradation along alpine elevation gradients better than other climatic or edaphic parameters
This study thus highlights that while most variables, such as temperature or humidity, as well as soil microbial activity, do impact decomposition process, in combination with the measured pedo-climatic niche, solar radiation, very likely by means of indirect effects, best captures variation in OM degradation. For instance, high solar radiation might favor photodegradation, in turn speeding up the decomposition activity of the local microbial communities. Future work should thus disentangle the synergistic effects of the unique local microbial community and solar radiation on OM decomposition across different habitats. (So...
Source: Frontiers in Microbiology - April 27, 2023 Category: Microbiology Source Type: research
Rooibos tea-in the cross fire of ROS, mitochondrial dysfunction and loss of proteostasis-positioned for healthy aging
Biogerontology. 2023 Feb 13. doi: 10.1007/s10522-022-10012-z. Online ahead of print.ABSTRACTImpaired mitochondrial function and loss of cellular proteostasis control are key hallmarks of aging and are implicated in the development of neurodegenerative diseases. A common denominator is the cell's inability to handle reactive oxygen species (ROS), leading to major downstream oxidative damage that exacerbates neuronal dysfunction. Although we have progressed in understanding the molecular defects associated with neuronal aging, many unanswered questions remain. How much ROS is required to serve cellular function before it bec...
Source: Biogerontology - February 13, 2023 Category: Geriatrics Authors: Catherine Smit Sholto De Wet Tamryn Barron Ben Loos Source Type: research
Rooibos tea-in the cross fire of ROS, mitochondrial dysfunction and loss of proteostasis-positioned for healthy aging
Biogerontology. 2023 Feb 13. doi: 10.1007/s10522-022-10012-z. Online ahead of print.ABSTRACTImpaired mitochondrial function and loss of cellular proteostasis control are key hallmarks of aging and are implicated in the development of neurodegenerative diseases. A common denominator is the cell's inability to handle reactive oxygen species (ROS), leading to major downstream oxidative damage that exacerbates neuronal dysfunction. Although we have progressed in understanding the molecular defects associated with neuronal aging, many unanswered questions remain. How much ROS is required to serve cellular function before it bec...
Source: Biogerontology - February 13, 2023 Category: Geriatrics Authors: Catherine Smit Sholto De Wet Tamryn Barron Ben Loos Source Type: research
Effects of Aspalathin on Insulin Resistance and Mitochondrial Dysfunction in Cultured Skeletal Muscle Cells - Potential Implications for Type 2 Diabetes Mellitus Therapy
Physiol Res. 2022 Aug 31. Online ahead of print.ABSTRACTNatural compounds may bare promising therapeutic benefits against metabolic diseases such as type 2 diabetes mellitus (T2DM), which are characterized by a state of insulin resistance and mitochondrial dysfunction. Here, we examined the cellular mechanisms by which aspalathin, a dihydrochalcone C-glucoside unique to rooibos, may ameliorate palmitate-induced insulin resistance and mitochondrial dysfunction in cultured C2C12 myotubules. This current study demonstrated that aspalathin remains effective in improving glucose uptake in insulin-resistant skeletal muscle cells...
Source: Physiological Research - September 1, 2022 Category: Physiology Authors: S E Mazibuko-Mbeje S Xh Mthembu C Jf Muller K Ziqubu N Muvhulawa R V Modibedi L Tiano P V Dludla Source Type: research
Effects of Aspalathin on Insulin Resistance and Mitochondrial Dysfunction in Cultured Skeletal Muscle Cells - Potential Implications for Type 2 Diabetes Mellitus Therapy
Physiol Res. 2022 Aug 31. Online ahead of print.ABSTRACTNatural compounds may bare promising therapeutic benefits against metabolic diseases such as type 2 diabetes mellitus (T2DM), which are characterized by a state of insulin resistance and mitochondrial dysfunction. Here, we examined the cellular mechanisms by which aspalathin, a dihydrochalcone C-glucoside unique to rooibos, may ameliorate palmitate-induced insulin resistance and mitochondrial dysfunction in cultured C2C12 myotubules. This current study demonstrated that aspalathin remains effective in improving glucose uptake in insulin-resistant skeletal muscle cells...
Source: Physiological Research - September 1, 2022 Category: Physiology Authors: S E Mazibuko-Mbeje S Xh Mthembu C Jf Muller K Ziqubu N Muvhulawa R V Modibedi L Tiano P V Dludla Source Type: research
