Targeting reactive oxygen species (ROS) to combat the age-related loss of muscle mass and function.
Abstract The loss of muscle mass and function with age, termed sarcopenia, is an inevitable process, which has a significant impact on quality of life. During ageing we observe a progressive loss of total muscle fibres and a reduction in cross-sectional area of the remaining fibres, resulting in a significant reduction in force output. The mechanisms which underpin sarcopenia are complex and poorly understood, ranging from inflammation, dysregulation of protein metabolism and denervation. However, there is significant evidence to demonstrate that modified ROS generation, redox dis-homeostasis and mitochondrial dys...
Source: Biogerontology - May 23, 2020 Category: Geriatrics Authors: Thoma A, Akter-Miah T, Reade RL, Lightfoot AP Tags: Biogerontology Source Type: research
Longevity-promoting efficacies of rutin in high fat diet fed Drosophila melanogaster.
This study aimed to understand whether rutin could exhibit similar longevity promoting effects in flies fed with a high fat diet (HFD). In this study, wild type Canton-S males and females were reared on high fat diet (HFD) treated with or without rutin at different doses (100-800 µM) and assessed for survival, food intake, fecundity, locomotion, development, resistance to various forms of stresses and relative mRNA expression of specific genes associated with ageing, namely dFoxO, MnSod, Cat, dTsc1, dTsc2, Thor, dAtg1, dAtg5, dAtg7 and dTor. Rutin at only 400 µM significantly improved survival in males fed with...
Source: Biogerontology - May 20, 2020 Category: Geriatrics Authors: Chattopadhyay D, Thirumurugan K Tags: Biogerontology Source Type: research
Functional alterations and transcriptomic changes during zebrafish cardiac aging.
In this study we first validated the effectiveness of a panel of aging-related markers and revealed their spatial-temporal specificity. Using these markers, we discovered that cardiac aging in zebrafish initiated at mid-age around 24 months, followed by a gradual progression marked with increased DNA damage, inflammatory response and reduced mitochondrial function. Furthermore, we showed aging-related expression profile change in zebrafish hearts was similar to that in rat hearts. Overall, our results provide a deeper insight into the cardiac aging process in zebrafish, which will set up foundation for generating nove...
Source: Biogerontology - May 5, 2020 Category: Geriatrics Authors: Shao X, Fu Y, Ma J, Li X, Lu C, Zhang R Tags: Biogerontology Source Type: research
Epigenetic changes during ageing and their underlying mechanisms.
Abstract As life expectancy increases worldwide, ageing and age-related diseases arise as a major issue for societies around the globe. Understanding the biological mechanisms underlying the ageing process is thus instrumental for the development of efficient interventions aimed to prevent and treat age-related conditions. Current knowledge in the biogerontology field points to epigenetics as a critical component of the ageing process, not only by serving as a bona-fide marker of biological age but also by controlling and conferring inheritability to cellular and organismal ageing. This is reflected by a myriad of...
Source: Biogerontology - April 30, 2020 Category: Geriatrics Authors: Braga DL, Mousovich-Neto F, Tonon-da-Silva G, Salgueiro WG, Mori MA Tags: Biogerontology Source Type: research
The redox environment and mitochondrial dysfunction in age-related skeletal muscle atrophy.
Abstract Medical advancements have extended human life expectancy, which is not always accompanied by an improved quality of life or healthspan. A decline in muscle mass and function is a consequence of ageing and can result in a loss of independence in elderly individuals while increasing their risk of falls. Multiple cellular pathways have been implicated in age-related muscle atrophy, including the contribution of reactive oxygen species (ROS) and disrupted redox signalling. Aberrant levels of ROS disrupts the redox environment in older muscle, potentially disrupting cellular signalling and in some cases blunti...
Source: Biogerontology - April 22, 2020 Category: Geriatrics Authors: Shally A, McDonagh B Tags: Biogerontology Source Type: research
Sirtuins family as a target in endothelial cell dysfunction: implications for vascular ageing.
Abstract The vascular endothelium is a protective barrier between the bloodstream and the vasculature that may be disrupted by different factors such as the presence of diseased states. Diseases like diabetes and obesity pose a great risk toward endothelial cell inflammation and oxidative stress, leading to endothelial cell dysfunction and thereby cardiovascular complications such as atherosclerosis. Sirtuins are NAD+-dependent histone deacetylases that are implicated in the pathophysiology of cardiovascular diseases, and they have been identified to be important regulators of endothelial cell function. A handful ...
Source: Biogerontology - April 13, 2020 Category: Geriatrics Authors: Zhang HN, Dai Y, Zhang CH, Omondi AM, Ghosh A, Khanra I, Chakraborty M, Yu XB, Liang J Tags: Biogerontology Source Type: research
Age-related chemokine alterations affect IgA secretion and gut immunity in female mice.
Abstract The chemokines CCL25 and CCL28, which promote immune cell migration, are primarily expressed in the small and large intestines and play critical roles in sustaining gut immunity. In particular, these chemokines are closely related to intestinal IgA secretion. However, there is no research regarding the effects of aging on CCL25 and CCL28 expression and function. Therefore, in the present study, we investigated the effects of aging on production of CCL25 and CCL28, and on gut immunity, especially IgA secretion, using young and aged female mice. By aging, the levels of small intestinal mRNA and protein of C...
Source: Biogerontology - April 10, 2020 Category: Geriatrics Authors: Nagafusa H, Sayama K Tags: Biogerontology Source Type: research
Novel biomarkers for the evaluation of aging-induced proteinopathies.
tay U Abstract Proteinopathies are characterized by aging related accumulation of misfolded protein aggregates. Irreversible covalent modifications of aging proteins may significantly affect the native three dimentional conformation of proteins, alter their function and lead to accumulation of misfolded protein as dysfunctional aggregates. Protein misfolding and accumulation of aberrant proteins are known to be associated with aging-induced proteinopathies such as amyloid ß and tau proteins in Alzheimer's disease, α-synuclein in Parkinson's disease and islet amyloid polypeptides in Type 2 diabetes mell...
Source: Biogerontology - April 9, 2020 Category: Geriatrics Authors: Yanar K, Atayik MC, Simsek B, Çakatay U Tags: Biogerontology Source Type: research
Therapeutic effects of hydro-alcoholic leaf extract of Withania somnifera on age-induced changes in daily rhythms of Sirt1, Nrf2 and Rev-erb α in the SCN of male Wistar rats.
Therapeutic effects of hydro-alcoholic leaf extract of Withania somnifera on age-induced changes in daily rhythms of Sirt1, Nrf2 and Rev-erbα in the SCN of male Wistar rats. Biogerontology. 2020 Apr 05;: Authors: Kukkemane K, Jagota A Abstract The temporal expression pattern of the circadian clock genes are known to be altered/attenuated with advance in age. Withania somnifera (WS) essentially consists of numerous active constituents including withanolides is known to have antioxidant, anti-inflammatory and adaptogenic properties. We have earlier demonstrated therapeutic effects of hydro-alcohol...
Source: Biogerontology - April 5, 2020 Category: Geriatrics Authors: Kukkemane K, Jagota A Tags: Biogerontology Source Type: research
The roles of MTOR and miRNAs in endothelial cell senescence.
Abstract Accumulation of senescent cells in vascular endothelium is known to contribute to vascular aging and increases the risk of developing cardiovascular diseases. The involvement of classical pathways such as p53/p21 and p16/pRB in cellular senescence are well described but there are emerging evidence supporting the increasingly important role of mammalian target of rapamycin (MTOR) as driver of cellular senescence via these pathways or other effector molecules. MicroRNAs (miRNAs) are a highly conserved group of small non-coding RNAs (18-25 nucleotides), instrumental in modulating the expression of targe...
Source: Biogerontology - April 3, 2020 Category: Geriatrics Authors: Khor ES, Wong PF Tags: Biogerontology Source Type: research
Effects of lifelong exercise and aging on the blood metabolic fingerprint of rats.
In conclusion, moderate-intensity exercise for life or half-life had strong and potentially healthful effects on body weight and (partly) appetite, as well as on some blood metabolites. The effects of aging on the rat blood metabolome seemed to be stronger than those of exercise. PMID: 32222870 [PubMed - as supplied by publisher] (Source: Biogerontology)
Source: Biogerontology - March 29, 2020 Category: Geriatrics Authors: Tzimou A, Benaki D, Nikolaidis S, Mikros E, Taitzoglou I, Mougios V Tags: Biogerontology Source Type: research
The sciences of healthy aging await a theory of health.
Abstract Debates in fields studying the biological aspects of aging and longevity, such as biogerontology, are often split between 'anti-aging' approaches aimed largely at treating diseases and those focusing more on maintaining, promoting, and even enhancing health. However, it is far from clear what this 'health' is that would be maintained, promoted, or enhanced. Interestingly, what few have yet to fully reflect on is that there is still no theory of health within the health or aging sciences that would provide an integrative explanatory framework akin to other scientific theories. After clarifying why such a t...
Source: Biogerontology - March 19, 2020 Category: Geriatrics Authors: Sholl J Tags: Biogerontology Source Type: research
Expression of human HSP27 in yeast extends replicative lifespan and uncovers a hormetic response.
Abstract Human HSP27 is a small heat shock protein that modulates the ability of cells to respond to heat shock and oxidative stress, and also functions as a chaperone independent of ATP, participating in the proteasomal degradation of proteins. The expression of HSP27 is associated with survival in mammalian cells. In cancer cells, it confers resistance to chemotherapy; in neurons, HSP27 has a positive effect on neuronal viability in models of Alzheimer's and Parkinson's diseases. To better understand the mechanism by which HSP27 expression contributes to cell survival, we expressed human HSP27 in the budding yea...
Source: Biogerontology - March 18, 2020 Category: Geriatrics Authors: Camandona VL, Rios-Anjos RM, Alegria TGP, Pereira F, Bicev RN, da Cunha FM, Digiampietri LA, de Barros MH, Netto LES, Ferreira-Junior JR Tags: Biogerontology Source Type: research
Characterisation of the positive effects of mild stress on ageing and resistance to stress.
This article summarises these results. PMID: 32189113 [PubMed - as supplied by publisher] (Source: Biogerontology)
Source: Biogerontology - March 18, 2020 Category: Geriatrics Authors: Le Bourg É Tags: Biogerontology Source Type: research
17 β-estradiol inhibits H2O2-induced senescence in HUVEC cells through upregulating SIRT3 expression and promoting autophagy.
In this study, we examined the roles of SIRT3 in 17β-E2-induced autophagy and 17β-E2-mediated inhibition of hydrogen peroxide (H2O2)-induced senescence in Human umbilical vein endothelial cells (HUVEC). Cellular senescence was measured by immunoblot analysis with antibodies against phosphorylated Rb and senescence-associated β-galactosidase staining. Immunoblot analysis with antibodies against LC3 and p62 was performed to determine autophagy flux. Our findings show that 17β-E2 activates SIRT3 promoter and upregulates SIRT3 gene expression in HUVEC cells. siRNA-mediated silencing of SIRT3 gene expression...
Source: Biogerontology - March 14, 2020 Category: Geriatrics Authors: Xiang X, Huang J, Song S, Wang Y, Zeng Y, Wu S, Ruan Y Tags: Biogerontology Source Type: research
Natural anti-aging skincare: role and potential.
Abstract The deterioration of the skin morphology and physiology is the first and earliest obvious harbinger of the aging process which is progressively manifested with increasing age. Such deterioration affects the vital functions of the skin such as homeodynamic regulation of body temperature, fluid balance, loss of electrolytes and proteins, production of vitamin D, waste removal, immune surveillance, sensory perception, and protection of other organs against deleterious environmental factors. There are, however, harmful chemicals and toxins found in everyday cosmetics that consumers are now aware of. Thus, the...
Source: Biogerontology - March 11, 2020 Category: Geriatrics Authors: Ahmed IA, Mikail MA, Zamakshshari N, Abdullah AH Tags: Biogerontology Source Type: research
Informative frailty indices from binarized biomarkers.
Abstract Frailty indices (FIs) based on continuous valued health data, such as obtained from blood and urine tests, have been shown to be predictive of adverse health outcomes. However, creating FIs from such biomarker data requires a binarization treatment that is difficult to standardize across studies. In this work, we explore a "quantile" methodology for the generic treatment of biomarker data that allows us to construct an FI without preexisting medical knowledge (i.e. risk thresholds) of the included biomarkers. We show that our quantile approach performs as well as, or even slightly better than, e...
Source: Biogerontology - March 10, 2020 Category: Geriatrics Authors: Stubbings G, Farrell S, Mitnitski A, Rockwood K, Rutenberg A Tags: Biogerontology Source Type: research
Dysregulations of mitochondrial quality control and autophagic flux at an early age lead to progression of sarcopenia in SAMP8 mice.
Abstract The senescence-accelerated mouse (SAM) prone 8 (SAMP8) has been demonstrated for muscular aging research including sarcopenia, but its underlying mechanisms remain scarce. Physiological indices and histology of skeletal muscle were analyzed in SAMP8 mice at different ages. SAMP8 mice exhibited typical features of sarcopenia at 40 weeks of age and were more time-efficient than that at 88 weeks of age in bothSAM resistant 1 (SAMR1) and C57BL/6 mice. Increase in FoxO3a-mediated transcription of Atrogin-1 and MuRF1 and decrease in phosphorylated mTOR/P70s6k were observed at week 40 in SAMP8 mice. High oxidati...
Source: Biogerontology - March 4, 2020 Category: Geriatrics Authors: Liu HW, Chang YC, Chan YC, Hu SH, Liu MY, Chang SJ Tags: Biogerontology Source Type: research
The crosstalk of NAD, ROS and autophagy in cellular health and ageing.
Abstract Cellular adaptation to various types of stress requires a complex network of steps that altogether lead to reconstitution of redox balance, degradation of damaged macromolecules and restoration of cellular metabolism. Advances in our understanding of the interplay between cellular signalling and signal translation paint a complex picture of multi-layered paths of regulation. In this review we explore the link between cellular adaptation to metabolic and oxidative stresses by activation of autophagy, a crucial cellular catabolic pathway. Metabolic stress can lead to changes in the redox state of nicotinami...
Source: Biogerontology - March 3, 2020 Category: Geriatrics Authors: Sedlackova L, Korolchuk VI Tags: Biogerontology Source Type: research
Bioinformatic framework for analysis of transcription factor changes as the molecular link between replicative cellular senescence signaling pathways and carcinogenesis.
Abstract Cellular senescence is a natural condition of irreversible cell cycle arrest and apoptotic resistance that occurs in cells exposed to various stress factors, such as replicative stress or overexpression of oncogenes. Unraveling the complex regulation of senescence in cells is essential to strengthen senescence-related therapeutic approaches in cancer, as cellular senescence plays a dual role in tumorigenesis, having both anti- and pro-tumorigenic effects. In our study we created a model of replicative cellular senescence, based on transcriptomic data, including an extra intermediate time-point prior to ce...
Source: Biogerontology - February 26, 2020 Category: Geriatrics Authors: Binenbaum I, Lefaki M, Chondrogianni N, Chatziioannou A Tags: Biogerontology Source Type: research
MicroRNAs, DNA damage response and ageing.
Abstract Ageing is a multifactorial and integrated gradual deterioration affecting the most of biological process of cells. MiRNAs are differentially expressed in the cellular senescence and play important role in regulating of genes expression involved in features of ageing. The perception of miRNAs functions in ageing regulation can be useful in clarifying the mechanisms underlying ageing and designing of therapeutic strategies. The preservation of genomic integrity through DNA damage response (DDR) is related to the process of cellular senescence. The recent studies have shown that miRNAs has directly regulated...
Source: Biogerontology - February 18, 2020 Category: Geriatrics Authors: Majidinia M, Mir SM, Mirza-Aghazadeh-Attari M, Asghari R, Kafil HS, Safa A, Mahmoodpoor A, Yousefi B Tags: Biogerontology Source Type: research
Alzheimer's disease: targeting the glutamatergic system.
Abstract Alzheimer's disease (AD) is a debilitating neurodegenerative disease that causes a progressive decline in memory, language and problem solving. For decades mechanism-based therapies have primarily focused on amyloid β (Aβ) processing and pathways that govern neurofibrillary tangle generation. With the potential exception to Aducanumab, a monotherapy to target Aβ, clinical trials in these areas have been challenging and have failed to demonstrate efficacy. Currently, the prescribed therapies for AD are those that target the cholinesterase and glutamatergic systems that can moderately reduce ...
Source: Biogerontology - February 11, 2020 Category: Geriatrics Authors: Conway ME Tags: Biogerontology Source Type: research
Preadipocyte secretory factors differentially modulate murine macrophage functions during aging which are reversed by the application of phytochemical EGCG.
Abstract The present study aimed at evaluating the role of senescent cell microenvironment as an extrinsic causal factor for altered age-associated macrophage functions, and that whether such changes could be ameliorated by the application of tea catechin epigallocatechin gallate (EGCG). To ascertain this, we analyzed the impact of secretory metabolites of proliferating (P) and senescent (S) preadipocyte cells on the induction of phenotypic and functional characteristics associated with aging in macrophages isolated from young (YM) and old (OM) C57BL/6J mice. The role of EGCG as alleviator of preadipocyte media-in...
Source: Biogerontology - February 10, 2020 Category: Geriatrics Authors: Kumar R, Sharma A, Padwad Y, Sharma R Tags: Biogerontology Source Type: research
D-Galactose-induced oxidative stress and mitochondrial dysfunction in the cochlear basilar membrane: an in vitro aging model.
In this study, we cultured the CBM, which was dissected from the cochlea of the C57BL/6 mice 5 days after birth, in a medium containing 20 mg/mL, 40 mg/mL, or 60 mg/mL D-galactose (D-gal). Compared with the control group, the levels of senescence-associated β-galactosidase were increased in a concentration-dependent manner in the CBM of the D-gal groups. In addition, levels of the mitochondrial superoxide and patterns of an age-related mitochondrial DNA3860-bp deletion were significantly increased. The ATP levels and the membrane potential of the mitochondrial were significantly decreased in the CBM of the D-gal group...
Source: Biogerontology - February 5, 2020 Category: Geriatrics Authors: Guo B, Guo Q, Wang Z, Shao JB, Liu K, Du ZD, Gong SS Tags: Biogerontology Source Type: research
Correction to: Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila.
Abstract The article Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila, written by Dmytro V. Gospodaryov. Olha M. Strilbytska. Uliana V. Semaniuk. Natalia V. Perkhulyn. Bohdana M. Rovenko. Ihor S. Yurkevych. Ana G. Barata. Tobias P. Dick. Oleh V. Lushchak and Howard T. Jacobs, was originally published electronically on the publisher's internet portal on 20 November 2019 without open access. With the author(s)' decision to opt for Open Choice the copyright of the article changed on 27 January 2020 to © The Author(s) 2020 and the article is forthwith distribut...
Source: Biogerontology - January 27, 2020 Category: Geriatrics Authors: Gospodaryov DV, Strilbytska OM, Semaniuk UV, Perkhulyn NV, Rovenko BM, Yurkevych IS, Barata AG, Dick TP, Lushchak OV, Jacobs HT Tags: Biogerontology Source Type: research
Coix seed oil prolongs lifespan and enhances stress resistance in Caenorhabditis elegans.
Abstract Coix seed oil (CSO) has many beneficial effects, but there is limited research on its influence on the processes and mechanisms related to senescence. Here, we used Caenorhabditis elegans as an in vivo model to investigate CSO's bioeffects on longevity. CSO (1 mg/mL) significantly extended the mean lifespan of C. elegans by over 22.79% and markedly improved stress resistance. Gene-specific mutant studies showed that the CSO-mediated increase in life expectancy was dependent on mev-1, hsf-1 and daf-16, but not daf-2. Furthermore, CSO significantly upregulated stress-inducible genes, including daf-16 a...
Source: Biogerontology - January 20, 2020 Category: Geriatrics Authors: Chen XY, Liao DC, Yu YT, Wei CM, Xuan LY, Li S, Wang HB Tags: Biogerontology Source Type: research
Phosphatidylserine modulates response to oxidative stress through hormesis and increases lifespan via DAF-16 in Caenorhabditis elegans.
This study reports the anti-oxidative stress and anti-aging effect of phosphatidylserine for the first time at the organismal level and proposes possible underlying mechanisms. PMID: 31915963 [PubMed - as supplied by publisher] (Source: Biogerontology)
Source: Biogerontology - January 8, 2020 Category: Geriatrics Authors: Kim BK, Park SK Tags: Biogerontology Source Type: research
Oxidative stress resistance in a short-lived Neotropical annual killifish.
hik L Abstract Oxidative stress plays an important role in the evolution of aging and life history. High investments in life-history traits and environmental conditions can be associated with increased oxidative stress and aging process. However, to date, most studies that investigated variations in oxidative status were performed with long-lived vertebrates. Studies with short-lived vertebrates in wild are nonexistent. Annual killifishes have the shortest lifespans among vertebrates and inhabit temporary ponds subject to large variations in environmental conditions. In this sense, we investigated whether the high...
Source: Biogerontology - December 20, 2019 Category: Geriatrics Authors: Godoy RS, Lanés LEK, Castro BD, Weber V, Wingen N, Pires MM, Oliveira GT, Maltchik L Tags: Biogerontology Source Type: research
Aberrant mitochondrial function in ageing and cancer.
Abstract Alterations in mitochondrial metabolism have been described as one of the major hallmarks of both ageing cells and cancer. Age is the biggest risk factor for the development of a significant number of cancer types and this therefore raises the question of whether there is a link between age-related mitochondrial dysfunction and the advantageous changes in mitochondrial metabolism prevalent in cancer cells. A common underlying feature of both ageing and cancer cells is the presence of somatic mutations of the mitochondrial genome (mtDNA) which we postulate may drive compensatory alterations in mitochondria...
Source: Biogerontology - December 4, 2019 Category: Geriatrics Authors: Whitehall JC, Greaves LC Tags: Biogerontology Source Type: research
Goat milk attenuates mimetic aging related memory impairment via suppressing brain oxidative stress, neurodegeneration and modulating neurotrophic factors in D-galactose-induced aging model.
In this study, the efficiency of goat milk to protect against memory decline was tested. Fifty-two male Sprague-Dawley rats were randomly divided into four groups: (i) control group, (ii) goat milk treated group, (iii) D-galactose treated group, and (iv) goat milk plus D-galactose treated group. Subcutaneous injections of D-galactose at 120 mg/kg and oral administrations of goat milk at 1 g/kg were chosen for the study. Goat milk and D-galactose were administered concomitantly for 6 weeks, while the control group received saline. After 6 weeks, novel object recognition and T-maze tests were performed to evaluate ...
Source: Biogerontology - December 2, 2019 Category: Geriatrics Authors: Safdar A, Zakaria R, Aziz CBA, Rashid U, Azman KF Tags: Biogerontology Source Type: research
Negative genetic correlation between longevity and its hormetic extension by dietary restriction in Drosophila melanogaster.
Abstract Longevity is a highly malleable trait which is influenced by many genetic and environmental factors including nutrition. Mild stress of dietary restriction (DR) is often beneficial by extending longevity in many organisms. Here, DR-induced effects on longevity were tested for genetic variation in a set of recombinant inbred lines (RIL) in D. melanogaster. Genetic variability was significant in the longevity response following a DR-treatment across RIL, with detrimental effects in several RIL but beneficial effects in other RIL. One quantitative trait locus (QTL) was consistently significant in the middle ...
Source: Biogerontology - November 30, 2019 Category: Geriatrics Authors: Gomez FH, Stazione L, Sambucetti P, Norry FM Tags: Biogerontology Source Type: research
Naive extrapolations, overhyped claims and empty promises in ageing research and interventions need avoidance.
Naive extrapolations, overhyped claims and empty promises in ageing research and interventions need avoidance. Biogerontology. 2019 Nov 27;: Authors: Rattan SIS Abstract Most proclamations about another wonder breakthrough and another imminent miracle treatment of ageing are usually overhyped claims and empty promises. It is not that the experimental science behind those claims is totally wrong or fake. But it is often a case of being ahistorical and ignoring the cumulated knowledge and understanding of the evolutionary and biological principles of ageing and longevity. Furthermore, remaining stu...
Source: Biogerontology - November 27, 2019 Category: Geriatrics Authors: Rattan SIS Tags: Biogerontology Source Type: research
Increase in hippocampal histone H3K9me3 is negatively correlated with memory in old male mice.
This study for the first time reports involvement of H3K9me3 in the regulation of neuronal IEGs during aging. Using novel object recognition and Y-maze test, the recognition and spatial memory was checked in male mice of different ages and it was found to decline in old. We have examined the expression of H3K9me3 specific histone methyltransferases and noted that only SUV39H1 (suppressor of variegation 3-9 homolog 1) increased significantly in old. Also the global H3K9me3 level was high in the hippocampus of old male mice. Further, chromatin immunoprecipitation assay revealed rise in H3K9me3 level at the promoter of IEGs i...
Source: Biogerontology - November 23, 2019 Category: Geriatrics Authors: Kushwaha A, Thakur MK Tags: Biogerontology Source Type: research
Alternative NADH dehydrogenase extends lifespan and increases resistance to xenobiotics in Drosophila.
Abstract Mitochondrial alternative NADH dehydrogenase (aNDH) was found to extend lifespan when expressed in the fruit fly. We have found that fruit flies expressing aNDH from Ciona intestinalis (NDX) had 17-71% lifespan prolongation on media with different protein-tocarbohydrate ratios except NDX-expressing males that had 19% shorter lifespan than controls on a high protein diet. NDX-expressing flies were more resistant to organic xenobiotics, 2,4-dichlorophenoxyacetic acid and alloxan, and inorganic toxicant potassium iodate, and partially to sodium molybdate treatments. On the other hand, NDX-expressing fli...
Source: Biogerontology - November 20, 2019 Category: Geriatrics Authors: Gospodaryov DV, Strilbytska OM, Semaniuk UV, Perkhulyn NV, Rovenko BM, Yurkevych IS, Barata AG, Dick TP, Lushchak OV, Jacobs HT Tags: Biogerontology Source Type: research
Metabolic remodelling of mice by hypoxic-hypercapnic environment: imitating the naked mole-rat.
Abstract We hypothesised that hypoxic-hypercapnic environment (HHE) could induce metabolic suppression and associated benefits for health and longevity, as observed in the naked-mole rat (NMR). We developed a model of self-produced HHE (similar to a natural habitat of NMRs), which is simple, reliable and natural, and does not require external sources of gases or complex technical equipment. Here, we showed for the first time that a chronic exposure of mice to HHE could be a unique tool for NMR-like metabolic remodeling, resulting in a long-term and substantial decrease in metabolic rate, body temperature, and food...
Source: Biogerontology - October 31, 2019 Category: Geriatrics Authors: Tolstun DA, Knyazer A, Tushynska TV, Dubiley TA, Bezrukov VV, Fraifeld VE, Muradian KK Tags: Biogerontology Source Type: research
Impact of curcumin on replicative and chronological aging in the Saccharomyces cerevisiae yeast.
Abstract Curcumin is a biologically active compound of vegetable origin which has a hormetic effect. Pro-health and anti-aging properties of curcumin have been known for years. The main benefit of curcumin is thought to be its anti-oxidative action. Despite vast amount of data confirming age-delaying activity of curcumin in various groups of organisms, so far little has been discovered about curcumin's impact on cell aging in the experimental model of the Saccharomyces cerevisiae budding yeast. We have been able to demonstrate that curcumin significantly increases oxidative stress and accelerates replicative ...
Source: Biogerontology - October 28, 2019 Category: Geriatrics Authors: Stępień K, Wojdyła D, Nowak K, Mołoń M Tags: Biogerontology Source Type: research
Age-related differences in physiology and survival of northern red-backed voles (Myodes rutilus) in captivity.
Abstract Age-related deterioration of physiological functions is one of the most evident manifestations of ageing. In wild populations of some species, including murid rodents, lifespans are substantially modified by environmental signals that affect an individual's response to such challenges as unfavourable climatic conditions, parasitic load etc. But the real impact of ageing on natural mortality of most species remains obscure. To clarify how age affects the responsiveness of organisms to environmental challenges, we performed longitudinal laboratory observations of wild-derived northern red-backed voles (Myod...
Source: Biogerontology - October 25, 2019 Category: Geriatrics Authors: Novikov EA, Kondratyuk EY, Polikarpov IA Tags: Biogerontology Source Type: research
The relationship between leukocyte telomere length and TERT, TRF1 single nucleotide polymorphisms in healthy people of different age groups.
Abstract Telomeres are nucleoprotein structures that cap the end of each chromosome and function to maintain genome stability. The length of telomeres is known to shorten with each cell division and it is well-established that telomere attrition is related to replicative capacity in vitro. Moreover, telomere loss is also correlated with the process of aging in vivo. That is why we aimed to find any associations of leukocyte telomere shortening with different age groups. We enrolled 291 healthy people in a study group. Samples of DNA from peripheral blood leukocytes were purified by th...
Source: Biogerontology - October 23, 2019 Category: Geriatrics Authors: Gedvilaite G, Vilkeviciute A, Kriauciuniene L, Banevičius M, Liutkeviciene R Tags: Biogerontology Source Type: research
Age and sex determine CD4+ T cell stimulatory and polarizing capacity of rat splenic dendritic cells.
Age and sex determine CD4+ T cell stimulatory and polarizing capacity of rat splenic dendritic cells. Biogerontology. 2019 Oct 23;: Authors: Stojić-Vukanić Z, Pilipović I, Bufan B, Stojanović M, Leposavić G Abstract The study investigated influence of sex and age on splenic myeloid dendritic cells (DCs) from Dark Agouti rats. Freshly isolated DCs from young males exhibited less mature phenotype and greater endocytic capacity compared with those from age-matched females. Upon LPS stimulation in vitro they were less potent in stimulating allogeneic CD4+ cells in mixed leukocyte reacti...
Source: Biogerontology - October 23, 2019 Category: Geriatrics Authors: Stojić-Vukanić Z, Pilipović I, Bufan B, Stojanović M, Leposavić G Tags: Biogerontology Source Type: research
Exercise training in ad libitum and food-restricted old rats: effects on metabolic and physiological parameters.
Abstract Aging is accompanied by a decline in the healthy function of multiple organs, leading to increased incidence and mortality from diseases such as cancer and inflammatory, cardiovascular and neurodegenerative diseases. Dietary restriction is the most effective experimental intervention known to consistently slow the aging process and with positive effects on health span in different organisms, from invertebrates to mammals. Age is also associated with progressive decline in physical activity levels in a wide range of animal species: therefore, regular physical exercise could represent a safe intervention to...
Source: Biogerontology - October 22, 2019 Category: Geriatrics Authors: Corbianco S, Dini M, Bongioanni P, Carboncini MC, Cavallini G Tags: Biogerontology Source Type: research
The role of DNA repair genes in radiation-induced adaptive response in Drosophila melanogaster is differential and conditional.
Abstract Studies in human and mammalian cell cultures have shown that induction of DNA repair mechanisms is required for the formation of stimulation effects of low doses of ionizing radiation, named "hormesis". Nevertheless, the role of cellular defense mechanisms in the formation of radiation-induced hormesis at the level of whole organism remains poorly studied. The aim of this work was to investigate the role of genes involved in different mechanisms and stages of DNA repair in radioadaptive response and radiation hormesis by lifespan parameters in Drosophila melanogaster. We studied genes that contr...
Source: Biogerontology - October 17, 2019 Category: Geriatrics Authors: Koval L, Proshkina E, Shaposhnikov M, Moskalev A Tags: Biogerontology Source Type: research
Why do bats live so long?-Possible molecular mechanisms.
Abstract Contrasting with several theories of ageing, bats are mammals with remarkable longevity despite their high metabolic rate, living on average three times more than other mammals of equal size. The question of how bats live a long time has attracted considerable attention, and they have thus been related to immortal fantasy characters like Dracula in the novel by Bram Stoker. Several ecological and physiological features, such as reduction in mortality risks, delayed sexual maturation and hibernation, have been linked to bats' long lifespan. However, there is still very little information about the molecula...
Source: Biogerontology - October 10, 2019 Category: Geriatrics Authors: Lagunas-Rangel FA Tags: Biogerontology Source Type: research
IL-6 deficiency attenuates p53 protein accumulation in aged male mouse hippocampus.
IL-6 deficiency attenuates p53 protein accumulation in aged male mouse hippocampus. Biogerontology. 2019 Oct 09;: Authors: Bialuk I, Cieślińska M, Kowalczuk O, Bonda TA, Nikliński J, Winnicka MM Abstract Our earlier studies demonstrated slower age-related memory decline in IL-6-deficient than in control mice. Therefore, in the present study we evaluated the effect of IL-6 deficiency and aging on expression of p53, connected with accumulation of age-related cellular damages, in hippocampus of 4- and 24-month-old IL-6-deficient C57BL/6J (IL-6KO) and wild type control (WT) mice. The accumulation ...
Source: Biogerontology - October 9, 2019 Category: Geriatrics Authors: Bialuk I, Cieślińska M, Kowalczuk O, Bonda TA, Nikliński J, Winnicka MM Tags: Biogerontology Source Type: research
Human ageing as a dynamic, emergent and malleable process: from disease-oriented to health-oriented approaches.
Abstract Over the decades, biogerontology has matured as a scientific discipline. Currently, a number of theoretical frameworks are available to researchers when interpreting empirical data. Despite the great progress that has been made, a comprehensive understanding of biological processes that shape ageing is lacking. Senescence is a dynamic, plastic and highly complex metaphenomenon whose aetiology remains unclear. The paucity of information notwithstanding, some researchers promote 'anti-ageing' drugs and formulae every now and again. The rationale behind this concept is that ageing can be reduced to a mixture...
Source: Biogerontology - October 8, 2019 Category: Geriatrics Authors: Chmielewski PP Tags: Biogerontology Source Type: research
Metformin prevents against oxidative stress-induced senescence in human periodontal ligament cells.
The objectives of the present study were to evaluate the effects of metformin on cellular senescence in human PDLCs (hPDLCs) under oxidative stress. hPDLCs were pretreated with metformin, followed by H2O2 exposure. The cell viability, oxidative damage, cellular senescence and osteogenic potential were detected. To inhibit autophagy, hPDLCs were treated with 3-methyladenine before metformin treatment. The present study revealed that H2O2 exposure inhibits proliferation, increased lysosomal β-galactosidase activity, augments reactive oxidative species (ROS) accumulation, elevates the oxidative damage, stimulates the exp...
Source: Biogerontology - September 26, 2019 Category: Geriatrics Authors: Kuang Y, Hu B, Feng G, Xiang M, Deng Y, Tan M, Li J, Song J Tags: Biogerontology Source Type: research
Hypergravity increases resistance to heat in dFOXO Drosophila melanogaster mutants and can lower FOXO translocation in wild-type males.
This study observed survival time of wild-type Drosophila melanogaster flies and dFOXO mutants exposed to 37 °C, a severe stress for flies, after they lived or not for 2 weeks in hypergravity (3 or 5 g), a mild stress with hormetic effects in flies. Hypergravity increased survival time of the mutants, this effect being less observed in wild-type flies. The heat stress increased dFOXO translocation similarly in all gravity groups in a wild-type strain, and hypergravity decreased dFOXO translocation similarly in heat-stressed or not heat-stressed males, no clear effect of the gravity level being observed i...
Source: Biogerontology - September 21, 2019 Category: Geriatrics Authors: Le Bourg E, Polesello C Tags: Biogerontology Source Type: research
D-Galactose-induced accelerated aging model: an overview.
Abstract To facilitate the process of aging healthily and prevent age-related health problems, efforts to properly understand aging mechanisms and develop effective and affordable anti-aging interventions are deemed necessary. Systemic administration of D-galactose has been established to artificially induce senescence in vitro and in vivo as well as for anti-aging therapeutic interventions studies. The aim of this article is to comprehensively discuss the use of D-galactose to generate a model of accelerated aging and its possible underlying mechanisms involved in different tissues/organs. PMID: 31538262 [Pu...
Source: Biogerontology - September 20, 2019 Category: Geriatrics Authors: Azman KF, Zakaria R Tags: Biogerontology Source Type: research
Development, lifespan and reproduction of spider mites exposed to predator-induced stress across generations.
This study highlighted the sex-difference of prey in response to predator-induced stress and sex-dependent parental effects on the offspring. PMID: 31535231 [PubMed - as supplied by publisher] (Source: Biogerontology)
Source: Biogerontology - September 19, 2019 Category: Geriatrics Authors: Li GY, Zhang ZQ Tags: Biogerontology Source Type: research
In silico analysis of human renin gene-gene interactions and neighborhood topologically associated domains suggests breakdown of insulators contribute to ageing-associated diseases.
Abstract Three-dimensional chromatin architecture and gene-gene interactions impact gene expression. We assembled this information, in silico, for the human renin gene (REN). We searched for chromatin contacts and boundaries and the locations of super-enhancers that are involved in cell specific differentiation. The REN promoter was connected via RNA polymerase II binding to promoters of 12 neighboring genes on chromosome 1q32.1 over a distance of 762,497 bp. This constitutes a regulatory archipelago. The genes formed 3 topologically associated domains (TADs), as follows: TAD1: ZC3H11A, SNRPE, LINC00303; SOX1...
Source: Biogerontology - September 13, 2019 Category: Geriatrics Authors: Donlon TA, Morris BJ Tags: Biogerontology Source Type: research
Correction to: Short-term dietary restriction in old mice rejuvenates the aging-induced structural imbalance of gut microbiota.
Abstract In the original publication of the article, the alphabets in figures were published in upper case and mismatched with the figure legends. The corrected figures and figure legends are given in this Correction. PMID: 31501966 [PubMed - as supplied by publisher] (Source: Biogerontology)
Source: Biogerontology - September 9, 2019 Category: Geriatrics Authors: Zeng T, Cui H, Tang D, Garside GB, Wang Y, Wu J, Tao Z, Zhang L, Tao S Tags: Biogerontology Source Type: research