The role of endoplasmic reticulum stress-related genes in the diagnosis and subtyping of non-alcoholic fatty liver disease
In conclusion, ERS is closely associated with the development of NAFLD. We established a promising and SVM-based predictive model to assess the risk of disease in patients with ERS subtypes and NAFLD.PMID:38477602 | DOI:10.4149/gpb_2023042 (Source: General Physiology and Biophysics)
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Zihao Guo Xiaoxiao Yu Zhihao Fang Kai Yang Changxu Liu Zhichao Dong Chang Liu Source Type: research
ANGPT1 promotes M1 macrophage polarization and inhibits lung adenocarcinoma progression by inhibiting the TGF- β signalling pathway
Gen Physiol Biophys. 2024 Mar;43(2):121-138. doi: 10.4149/gpb_2024001.ABSTRACTImmune cells in the immune microenvironment of lung adenocarcinoma (LUAD) are involved in tumour progression. The aim of this study was to investigate the molecular mechanisms of immune infiltration-related genes in LUAD. The GEO, GeneCards, BioGPS and Genehopper databases were utilized to screen for immune infiltration-related differentially expressed genes (DEGs) in LUAD. Protein-protein interaction (PPI) network construction and survival analysis were performed in the Kaplan-Meier database to identify hub genes. The TIMER 2.0 database was used...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Gang Liu Hao Zhang Source Type: research
Network pharmacology and pharmacological evaluation of Wenzheng Jiedu Powder Modified Formula for neuropathic pain relief
This study aimed to elucidate the mechanism of Wenzheng Jiedu Powder Modified Formula (WJPMF) in treating neuropathic pain (NP). Network pharmacology and experimental verification were integrated to explore the therapeutic effects and key targets of WJPMF. Active components, corresponding target genes, and absorption, distribution, metabolism, and excretion (ADME) genes of WJPMF against NP were screened from public databases. Network analysis and molecular docking were conducted to identify key targets and verify binding abilities. In vivo experiments were performed on spared nerve injury (SNI) rats to assess the analgesic...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Yanping Li Feng Yang Wanying Lang Qi An Zhi Pan Wenyu Shi Xiaowei Shi Source Type: research
Daidzein protects endothelial cells against high glucose-induced injury through the dual-activation of PPAR α and PPARγ
In this study, the cell proliferation was detected by EdU. The intrinsic activity and binding affinity of daidzein for human PPARs (hPPARs) were estimated by transactivation reporter gene test and HPLC-UV method, respectively. Daidzein significantly reversed high glucose (HG, at 30 mmol/l)-induced injury in HUVECs, which was inhibited by both PPARα and PPARγ antagonist, but no PPARβ antagonist. Daidzein selectively activated hPPARα and hPPARγ1, but weakly hPPARβ. Additionally, daidzein also bound to both hPPARα and hPPARγ1. The findings suggested that daidzein may be a PPARα and PPARγ dual-agonist. The ameliorati...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Xuemei Yang Xinhui Jiang Changqing Liu Chuang Yang Sheng Yao Hongmei Qiu Junxia Yang Ke Wu Hong Liao Qingsong Jiang Source Type: research
TRIM67 interacts with ENAH to regulate the apoptosis and autophagy of lung cancer cells
Gen Physiol Biophys. 2024 Mar;43(2):163-173. doi: 10.4149/gpb_2023037.ABSTRACTThe aim of this study was to further clarify the functional mechanism of the triangular 67 (TRIM67) gene in lung cancer cells. We detected the expression of TRIM67 in lung cancer cells by RT-qPCR and Western blot, transfected si-NC, si-TRIM67, and pcDNA-ENAH into the cells. The expression of TRIM67 and ENAH was detected by Western blot and immunofluorescence localization, and CO-IP and GST pull-down experiments verified the interaction. Flow cytometry, Western blot, and transmission electron microscopy (TEM) evaluated the apoptosis and autophagy ...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Rui Liu Kun Miao Ling Gao Ling He Source Type: research
miR-497-5p promoted neuronal injury in ischemic stroke by inhibiting the BDNF/TrkB/PI3K/Akt pathway
Gen Physiol Biophys. 2024 Mar;43(2):175-183. doi: 10.4149/gpb_2023038.ABSTRACTThe aim of this study was to investigate the molecular mechanism by which miR-497-5p regulates neuronal injury after ischemic stroke through the BDNF/TrkB/Akt signaling pathway. PC12 cells were used to construct a stroke injury model by oxygen-glucose deprivation/reoxygenation (OGD/R). The expression level of miR-497-5p was measured by RT-qPCR. CCK-8 kit was used to detect cell viability. Cell apoptosis and reactive oxygen species (ROS) were detected by flow cytometry. MDA and SOD detection kits were used to detect MDA content and SOD activity. A...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Chunyan Gong Xiaona He Guiliang Li Dayu Wang Yonghua Yang Yanping Shi Wenbing Su Yuanxian Wu Source Type: research
The role of endoplasmic reticulum stress-related genes in the diagnosis and subtyping of non-alcoholic fatty liver disease
In conclusion, ERS is closely associated with the development of NAFLD. We established a promising and SVM-based predictive model to assess the risk of disease in patients with ERS subtypes and NAFLD.PMID:38477602 | DOI:10.4149/gpb_2023042 (Source: General Physiology and Biophysics)
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Zihao Guo Xiaoxiao Yu Zhihao Fang Kai Yang Changxu Liu Zhichao Dong Chang Liu Source Type: research
ANGPT1 promotes M1 macrophage polarization and inhibits lung adenocarcinoma progression by inhibiting the TGF- β signalling pathway
Gen Physiol Biophys. 2024 Mar;43(2):121-138. doi: 10.4149/gpb_2024001.ABSTRACTImmune cells in the immune microenvironment of lung adenocarcinoma (LUAD) are involved in tumour progression. The aim of this study was to investigate the molecular mechanisms of immune infiltration-related genes in LUAD. The GEO, GeneCards, BioGPS and Genehopper databases were utilized to screen for immune infiltration-related differentially expressed genes (DEGs) in LUAD. Protein-protein interaction (PPI) network construction and survival analysis were performed in the Kaplan-Meier database to identify hub genes. The TIMER 2.0 database was used...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Gang Liu Hao Zhang Source Type: research
Network pharmacology and pharmacological evaluation of Wenzheng Jiedu Powder Modified Formula for neuropathic pain relief
This study aimed to elucidate the mechanism of Wenzheng Jiedu Powder Modified Formula (WJPMF) in treating neuropathic pain (NP). Network pharmacology and experimental verification were integrated to explore the therapeutic effects and key targets of WJPMF. Active components, corresponding target genes, and absorption, distribution, metabolism, and excretion (ADME) genes of WJPMF against NP were screened from public databases. Network analysis and molecular docking were conducted to identify key targets and verify binding abilities. In vivo experiments were performed on spared nerve injury (SNI) rats to assess the analgesic...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Yanping Li Feng Yang Wanying Lang Qi An Zhi Pan Wenyu Shi Xiaowei Shi Source Type: research
Daidzein protects endothelial cells against high glucose-induced injury through the dual-activation of PPAR α and PPARγ
In this study, the cell proliferation was detected by EdU. The intrinsic activity and binding affinity of daidzein for human PPARs (hPPARs) were estimated by transactivation reporter gene test and HPLC-UV method, respectively. Daidzein significantly reversed high glucose (HG, at 30 mmol/l)-induced injury in HUVECs, which was inhibited by both PPARα and PPARγ antagonist, but no PPARβ antagonist. Daidzein selectively activated hPPARα and hPPARγ1, but weakly hPPARβ. Additionally, daidzein also bound to both hPPARα and hPPARγ1. The findings suggested that daidzein may be a PPARα and PPARγ dual-agonist. The ameliorati...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Xuemei Yang Xinhui Jiang Changqing Liu Chuang Yang Sheng Yao Hongmei Qiu Junxia Yang Ke Wu Hong Liao Qingsong Jiang Source Type: research
TRIM67 interacts with ENAH to regulate the apoptosis and autophagy of lung cancer cells
Gen Physiol Biophys. 2024 Mar;43(2):163-173. doi: 10.4149/gpb_2023037.ABSTRACTThe aim of this study was to further clarify the functional mechanism of the triangular 67 (TRIM67) gene in lung cancer cells. We detected the expression of TRIM67 in lung cancer cells by RT-qPCR and Western blot, transfected si-NC, si-TRIM67, and pcDNA-ENAH into the cells. The expression of TRIM67 and ENAH was detected by Western blot and immunofluorescence localization, and CO-IP and GST pull-down experiments verified the interaction. Flow cytometry, Western blot, and transmission electron microscopy (TEM) evaluated the apoptosis and autophagy ...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Rui Liu Kun Miao Ling Gao Ling He Source Type: research
miR-497-5p promoted neuronal injury in ischemic stroke by inhibiting the BDNF/TrkB/PI3K/Akt pathway
Gen Physiol Biophys. 2024 Mar;43(2):175-183. doi: 10.4149/gpb_2023038.ABSTRACTThe aim of this study was to investigate the molecular mechanism by which miR-497-5p regulates neuronal injury after ischemic stroke through the BDNF/TrkB/Akt signaling pathway. PC12 cells were used to construct a stroke injury model by oxygen-glucose deprivation/reoxygenation (OGD/R). The expression level of miR-497-5p was measured by RT-qPCR. CCK-8 kit was used to detect cell viability. Cell apoptosis and reactive oxygen species (ROS) were detected by flow cytometry. MDA and SOD detection kits were used to detect MDA content and SOD activity. A...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Chunyan Gong Xiaona He Guiliang Li Dayu Wang Yonghua Yang Yanping Shi Wenbing Su Yuanxian Wu Source Type: research
The role of endoplasmic reticulum stress-related genes in the diagnosis and subtyping of non-alcoholic fatty liver disease
In conclusion, ERS is closely associated with the development of NAFLD. We established a promising and SVM-based predictive model to assess the risk of disease in patients with ERS subtypes and NAFLD.PMID:38477602 | DOI:10.4149/gpb_2023042 (Source: General Physiology and Biophysics)
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Zihao Guo Xiaoxiao Yu Zhihao Fang Kai Yang Changxu Liu Zhichao Dong Chang Liu Source Type: research
ANGPT1 promotes M1 macrophage polarization and inhibits lung adenocarcinoma progression by inhibiting the TGF- β signalling pathway
Gen Physiol Biophys. 2024 Mar;43(2):121-138. doi: 10.4149/gpb_2024001.ABSTRACTImmune cells in the immune microenvironment of lung adenocarcinoma (LUAD) are involved in tumour progression. The aim of this study was to investigate the molecular mechanisms of immune infiltration-related genes in LUAD. The GEO, GeneCards, BioGPS and Genehopper databases were utilized to screen for immune infiltration-related differentially expressed genes (DEGs) in LUAD. Protein-protein interaction (PPI) network construction and survival analysis were performed in the Kaplan-Meier database to identify hub genes. The TIMER 2.0 database was used...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Gang Liu Hao Zhang Source Type: research
Network pharmacology and pharmacological evaluation of Wenzheng Jiedu Powder Modified Formula for neuropathic pain relief
This study aimed to elucidate the mechanism of Wenzheng Jiedu Powder Modified Formula (WJPMF) in treating neuropathic pain (NP). Network pharmacology and experimental verification were integrated to explore the therapeutic effects and key targets of WJPMF. Active components, corresponding target genes, and absorption, distribution, metabolism, and excretion (ADME) genes of WJPMF against NP were screened from public databases. Network analysis and molecular docking were conducted to identify key targets and verify binding abilities. In vivo experiments were performed on spared nerve injury (SNI) rats to assess the analgesic...
Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Yanping Li Feng Yang Wanying Lang Qi An Zhi Pan Wenyu Shi Xiaowei Shi Source Type: research
