Putative identification of proopiomelanocortin and neuropeptide-Y neurons of the arcuate nucleus by their response to leptin: in vivo electrophysiology study in male and female rats
This study aimed at the direct electrophysiological examination of the effect of leptin on ARN neurons and to investigate potential sex-dimorphic changes. Wistar rats were anesthetized with urethane and the electrodes were inserted into the ARN. After a spontaneous active neuron was recorded for at least one minute, leptin was administered intravenously, and the firing activity of the same neuron was recorded for two additional minutes. It was found that approximately half of the ARN neurons had an excitatory, and another half an inhibitory response to the leptin administration. The excitability of the neurons with excitat...
Source: General Physiology and Biophysics - February 22, 2024 Category: Physiology Authors: Daniil Grinchii Viera Kominkova Eliyahu Dremencov Source Type: research
Putative identification of proopiomelanocortin and neuropeptide-Y neurons of the arcuate nucleus by their response to leptin: in vivo electrophysiology study in male and female rats
This study aimed at the direct electrophysiological examination of the effect of leptin on ARN neurons and to investigate potential sex-dimorphic changes. Wistar rats were anesthetized with urethane and the electrodes were inserted into the ARN. After a spontaneous active neuron was recorded for at least one minute, leptin was administered intravenously, and the firing activity of the same neuron was recorded for two additional minutes. It was found that approximately half of the ARN neurons had an excitatory, and another half an inhibitory response to the leptin administration. The excitability of the neurons with excitat...
Source: General Physiology and Biophysics - February 22, 2024 Category: Physiology Authors: Daniil Grinchii Viera Kominkova Eliyahu Dremencov Source Type: research
Putative identification of proopiomelanocortin and neuropeptide-Y neurons of the arcuate nucleus by their response to leptin: in vivo electrophysiology study in male and female rats
This study aimed at the direct electrophysiological examination of the effect of leptin on ARN neurons and to investigate potential sex-dimorphic changes. Wistar rats were anesthetized with urethane and the electrodes were inserted into the ARN. After a spontaneous active neuron was recorded for at least one minute, leptin was administered intravenously, and the firing activity of the same neuron was recorded for two additional minutes. It was found that approximately half of the ARN neurons had an excitatory, and another half an inhibitory response to the leptin administration. The excitability of the neurons with excitat...
Source: General Physiology and Biophysics - February 22, 2024 Category: Physiology Authors: Daniil Grinchii Viera Kominkova Eliyahu Dremencov Source Type: research
CircRSU1 alleviates LPS-induced human pulmonary microvascular endothelial cell injury by targeting miR-1224-5p/ITGA5 axis
Gen Physiol Biophys. 2024 Jan;43(1):1-11. doi: 10.4149/gpb_2023031.ABSTRACTTo investigate the potential functions and regulatory mechanism of circRSU1 on septic acute lung injury (sepsis-ALI) progression. We used lipopolysaccharide (LPS)-stimulated human pulmonary microvascular endothelial cells (HPMECs) to establish the cell model of sepsis-ALI in vitro. qRT-PCR and Western blotting were used for the detection of genes and proteins. The migration and tubulogenesis of HPMECs were assessed by transwell, wound healing, and tube formation assays. Inflammatory factors were detected by ELISA analysis. Cell permeability (PA) was...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Yongtao Cheng Fenggong Wang Cui Guo Shenghua Yuan Jianzhong Li Yuangang Zhang Source Type: research
Naringenin protects myocardial ischemia/reperfusion injury by regulating miR-24-3p to inhibit cell death-inducing p53 target 1 expression
In conclusion, our data suggest naringenin protects myocardial I/R injury partially through miR-24-3p/Cdip1 axis.PMID:38312031 | DOI:10.4149/gpb_2023035 (Source: General Physiology and Biophysics)
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Xiao Jin Ling Jin Bingxin Wu Danping Xu Source Type: research
Effect of quercetin administration during the first two weeks post-weaning on the development of non-alcoholic fatty liver disease and dyslipidaemia in Sprague Dawley rats fed a high fructose diet
Gen Physiol Biophys. 2024 Jan;43(1):25-36. doi: 10.4149/gpb_2023033.ABSTRACTHepatic steatosis and dyslipidaemia are associated with excessive fructose consumption. We investigated the effect of quercetin intake during the early pre-weaning period on metabolic dysfunction caused by a high fructose diet. Sprague Dawley rats, 21-day-old, were weaned onto standard rat chow and randomly allocated to four groups which either water or 20% fructose solution to drink with or without quercetin (100 mg/kg body mass). Quercetin was administered for two weeks. Thereafter, rats continued on their respective diets for six weeks without q...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Ramatsobane Tladi Kennedy H Erlwanger Janine Donaldson Source Type: research
Inactivation of the TGF- β1/ALK5 axis enhances club cell function and alleviates lung tissue damage to ameliorate COPD progression through the MEK/ERK signaling pathway
This study aimed to investigate the role of the TGF-β1/ALK5 signaling pathway in club cell function and COPD progression. C57BL/6J mice were divided into Normal group (exposed to fresh air) and COPD group (exposed to incremental cigarette smoke extract for 12 weeks). The COPD mice were further divided into COPD group, DMSO group, and LY2109761 group (injected with 150 mg/kg LY2109761, a TGF-β1 inhibitor). Tissue staining was used to assess lung damage, and the expression of CC16 was measured. The levels of inflammatory factors and DNA damage-related indicators were also measured. The involvement of the MEK/ERK signaling ...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Jing Tian Hui Ouyang Jie Wu Lu Wen Xiaoping Li Fangying Yang Hao Yuan Source Type: research
Histopathological findings in lung biopsies with usual interstitial pneumonia: Definition of a new classification score for histological fibrotic stages
The objective of this article is to describe and classify usual interstitial pneumonia (UIP) changes according to their relevance in the pathology of the idiopathic pulmonary fibrosis (IPF) process. In a cohort of 50 patients (25♀, 25♂) with UIP findings, the percentage ratio between fibrotic and preserved parts of the lungs was quantified. Three quantitative stages of fibrotic involvement of the lung parenchyma and concomitant changes were defined. These are initial (≤20%), advanced (21-40%), and diffuse (≥41%) fibrosis of the lungs. Histologically, temporal heterogeneity is predominant with thickened alveolar sep...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: M ária Makovická Adela Vrbensk á Peter Makovick ý Barbora Durcov á Jozef Škarda Vojt ěch Kamarád M ária Miklošová Kvetoslava Rim árová Patricie Michal čová Klaudia Kr áľová Jozef Muri Source Type: research
Combination therapy of metformin and atorvastatin against benzopyrene-induced lung cancer via inflammatory signaling pathway
In this study, we scrutinized the chemoprotective effect of the metformin and atorvastatin combination against benzo[a]pyrene (BaP)-induced lung cancer in mice of Swiss albino. BaP (50 mg/kg) was used for induction of lung cancer and mice were treated with metformin, atorvastatin or their combination. Metformin + atorvastatin combination significantly (p< 0.001) improved the body weight, liver weight, suppressed the lung weight and tumor incidence and altered the levels of immunocompetent cells, polyamines, lung tumor markers, lung parameters and antioxidant parameters, respectively. Metformin + atorvastatin combination...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Xuecong Ning Shusen Zhang Zhiguo Gao Aimin Li Source Type: research
Microbubbles activated by low-frequency ultrasound enhance the anti-tumor effects of curcumin in glioma cells by suppressing the TGF- β1/Smad/VEGF/NCAM signaling pathway
This study investigated whether microbubbles activated by low-frequency ultrasound enhanced the anti-tumor effects of curcumin in glioma cells. CCK8 proliferation assay, scratch migration assay, and transwell invasion assay were performed to estimate the proliferation, migration, and invasion rates of the glioma cells in blank control and different treatment groups, respectively. Quantitative RT-PCR (qRT-PCR) analysis was performed to determine the relative expression levels of VEGF and NCAM mRNAs in the various experimental groups. Western blotting was performed to determine the activity status of the TGF-β1/Smad signali...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Lixia Mei Zhen Zhang Xiaole Song Xiaodi Zhao Source Type: research
CircRSU1 alleviates LPS-induced human pulmonary microvascular endothelial cell injury by targeting miR-1224-5p/ITGA5 axis
Gen Physiol Biophys. 2024 Jan;43(1):1-11. doi: 10.4149/gpb_2023031.ABSTRACTTo investigate the potential functions and regulatory mechanism of circRSU1 on septic acute lung injury (sepsis-ALI) progression. We used lipopolysaccharide (LPS)-stimulated human pulmonary microvascular endothelial cells (HPMECs) to establish the cell model of sepsis-ALI in vitro. qRT-PCR and Western blotting were used for the detection of genes and proteins. The migration and tubulogenesis of HPMECs were assessed by transwell, wound healing, and tube formation assays. Inflammatory factors were detected by ELISA analysis. Cell permeability (PA) was...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Yongtao Cheng Fenggong Wang Cui Guo Shenghua Yuan Jianzhong Li Yuangang Zhang Source Type: research
Naringenin protects myocardial ischemia/reperfusion injury by regulating miR-24-3p to inhibit cell death-inducing p53 target 1 expression
In conclusion, our data suggest naringenin protects myocardial I/R injury partially through miR-24-3p/Cdip1 axis.PMID:38312031 | DOI:10.4149/gpb_2023035 (Source: General Physiology and Biophysics)
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Xiao Jin Ling Jin Bingxin Wu Danping Xu Source Type: research
Effect of quercetin administration during the first two weeks post-weaning on the development of non-alcoholic fatty liver disease and dyslipidaemia in Sprague Dawley rats fed a high fructose diet
Gen Physiol Biophys. 2024 Jan;43(1):25-36. doi: 10.4149/gpb_2023033.ABSTRACTHepatic steatosis and dyslipidaemia are associated with excessive fructose consumption. We investigated the effect of quercetin intake during the early pre-weaning period on metabolic dysfunction caused by a high fructose diet. Sprague Dawley rats, 21-day-old, were weaned onto standard rat chow and randomly allocated to four groups which either water or 20% fructose solution to drink with or without quercetin (100 mg/kg body mass). Quercetin was administered for two weeks. Thereafter, rats continued on their respective diets for six weeks without q...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Ramatsobane Tladi Kennedy H Erlwanger Janine Donaldson Source Type: research
Inactivation of the TGF- β1/ALK5 axis enhances club cell function and alleviates lung tissue damage to ameliorate COPD progression through the MEK/ERK signaling pathway
This study aimed to investigate the role of the TGF-β1/ALK5 signaling pathway in club cell function and COPD progression. C57BL/6J mice were divided into Normal group (exposed to fresh air) and COPD group (exposed to incremental cigarette smoke extract for 12 weeks). The COPD mice were further divided into COPD group, DMSO group, and LY2109761 group (injected with 150 mg/kg LY2109761, a TGF-β1 inhibitor). Tissue staining was used to assess lung damage, and the expression of CC16 was measured. The levels of inflammatory factors and DNA damage-related indicators were also measured. The involvement of the MEK/ERK signaling ...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: Jing Tian Hui Ouyang Jie Wu Lu Wen Xiaoping Li Fangying Yang Hao Yuan Source Type: research
Histopathological findings in lung biopsies with usual interstitial pneumonia: Definition of a new classification score for histological fibrotic stages
The objective of this article is to describe and classify usual interstitial pneumonia (UIP) changes according to their relevance in the pathology of the idiopathic pulmonary fibrosis (IPF) process. In a cohort of 50 patients (25♀, 25♂) with UIP findings, the percentage ratio between fibrotic and preserved parts of the lungs was quantified. Three quantitative stages of fibrotic involvement of the lung parenchyma and concomitant changes were defined. These are initial (≤20%), advanced (21-40%), and diffuse (≥41%) fibrosis of the lungs. Histologically, temporal heterogeneity is predominant with thickened alveolar sep...
Source: General Physiology and Biophysics - February 5, 2024 Category: Physiology Authors: M ária Makovická Adela Vrbensk á Peter Makovick ý Barbora Durcov á Jozef Škarda Vojt ěch Kamarád M ária Miklošová Kvetoslava Rim árová Patricie Michal čová Klaudia Kr áľová Jozef Muri Source Type: research
