Daidzein protects endothelial cells against high glucose-induced injury through the dual-activation of PPAR α and PPARγ

In this study, the cell proliferation was detected by EdU. The intrinsic activity and binding affinity of daidzein for human PPARs (hPPARs) were estimated by transactivation reporter gene test and HPLC-UV method, respectively. Daidzein significantly reversed high glucose (HG, at 30 mmol/l)-induced injury in HUVECs, which was inhibited by both PPARα and PPARγ antagonist, but no PPARβ antagonist. Daidzein selectively activated hPPARα and hPPARγ1, but weakly hPPARβ. Additionally, daidzein also bound to both hPPARα and hPPARγ1. The findings suggested that daidzein may be a PPARα and PPARγ dual-agonist. The amelioration of daidzein on HUVECs from hyperglycemia may be mediated by the activation of PPARα and PPARγ receptors.PMID:38477605 | DOI:10.4149/gpb_2023041

Source: General Physiology and Biophysics - March 13, 2024 Category: Physiology Authors: Xuemei Yang Xinhui Jiang Changqing Liu Chuang Yang Sheng Yao Hongmei Qiu Junxia Yang Ke Wu Hong Liao Qingsong Jiang Source Type: research