Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Production of a p65 < sup > fl/fl < /sup > /LysMCre mouse model with dysfunctional NF- κB signaling in bone marrow-derived macrophages
Innate Immun. 2023 Nov;29(8):171-185. doi: 10.1177/17534259231205993. Epub 2023 Oct 13.ABSTRACTHere, we describe the production and characterization of a novel p65fl/fl/LysMCre mouse model, which lacks canonical nuclear factor-kappaB member RelA/p65 (indicated as p65 hereafter) in bone marrow-derived macrophages. Cultured bone marrow-derived macrophages that lack p65 protein reveal NF-κB signaling deficiencies, a reduction in phagocytic ability, and reduced ability to produce nitrites. Despite abnormal bone marrow-derived macrophage function, p65fl/fl/LysMCre mice do not exhibit differences in naïve systemic immune profi...
Source: Innate Immunity - October 13, 2023 Category: Allergy & Immunology Authors: Ahmet K Korkaya Jeffrey Fischer Anthony Peppers Sean M Crosson Manira Rayamajhi Edward A Miao Albert S Baldwin Jennifer W Bradford Source Type: research
Molecules, Vol. 28, Pages 7075: Characterization of Spray-Dried Microcapsules of Paprika Oleoresin Induced by Ultrasound and High-Pressure Homogenization: Physicochemical Properties and Storage Stability
In this study, five primary emulsions of paprika oleoresin (PO, the natural colourant extracted from the fruit peel of Capsicum annuum L.) with different particle sizes (255&ndash;901.7 nm) were prepared using three industrialized pulverization-inducing techniques (stirring, ultrasound induction, and high-pressure homogenization). Subsequently, the PO emulsion was microencapsulated via spray drying. The effects of the different induction methods on the physicochemical properties, digestive behaviour, antioxidant activity, and storage stability of PO microencapsulated powder were investigated. The results showed tha...
Source: Molecules - October 13, 2023 Category: Chemistry Authors: Qionglian Zhang Yan Chen Fang Geng Xiaoyun Shen Tags: Article Source Type: research
This Mexican biologist is fighting to preserve his country ’s biodiversity—and his own legacy
Calakmul Biosphere Reserve on Mexico’s Yucatán Peninsula—
The mother bat screeches furiously as Rodrigo Medellín Legorreta grips her, a thumb under her chin to prevent her razor-sharp teeth from sinking into his gloved hand. “These creatures are feisty. They are witty,” he says. “And when they do manage to bite you, you scream, and you curse.” With the creature subdued, he gently pries a furry bulge from her chest. He hands the baby woolly false vampire bat (
Chrotopterus auritus
) to Ángel Torres Alcántara, an undergraduate on his first field trip to El Hormiguero, a Maya temple ruin at the ...
Source: Science of Aging Knowledge Environment - October 12, 2023 Category: Geriatrics Source Type: research
In silico and computational analysis of zinc finger motif-associated homeodomain (ZF-HD) family genes in chilli (Capsicum annuum L)
Zinc finger-homeodomain (ZHD) proteins are mostly expressed in plants and are involved in proper growth and development and minimizing biotic and abiotic stress. A recent study identified and characterized the... (Source: BMC Genomics)
Source: BMC Genomics - October 11, 2023 Category: Genetics & Stem Cells Authors: Md. Abir Ul Islam, Juthy Abedin Nupur, Muhammad Shafiq, Qurban Ali, Adnan Sami and Muhammad Adnan Shahid Tags: Research Source Type: research
Microscopic and metabolic investigations disclose the factors that lead to skin cracking in chili-type pepper fruit varieties
In conclusion, our data highlight cuticle thickness and epidermal cell density as two critical factors determining fruit skin susceptibility to cracking in chili-type pepper fruit.PMID:37799628 | PMC:PMC10548408 | DOI:10.1093/hr/uhad036 (Source: Cell Research)
Source: Cell Research - October 6, 2023 Category: Cytology Authors: Ofir Marinov Gal Nomberg Sutanni Sarkar Gulab Chand Arya Eldad Karavani Einat Zelinger Ekaterina Manasherova Hagai Cohen Source Type: research
Unleashing the bioactive potential of < em > Capsicum chinense < /em > cv Bhut Jolokia: a comparison of microwave- and ultrasound-mediated extraction techniques for lipophilic capsaicin
This study investigated the extraction of capsaicin from Capsicum chinense cv Bhut Jolokia using rice bran oil (RBO) as a 'green solvent' via microwave- and ultrasound-mediated techniques (MME and UME) as an alternative to hazardous organic solvents. Extraction conditions were optimised using MME at 180 and 300 W for 2 and 8 min and UME at 300 and 400 W for 10 and 30 min. The maximum capsaicin yield obtained was 12.47 mg/mL, with a corresponding gamma oryzanol content of 8.46 mg/mL. The peroxide value of the extract (capsaicin-infused RBO) was found to be within permissible limits as per Codex Alimentarius specifications. ...
Source: Natural Product Research - October 3, 2023 Category: Biochemistry Authors: Vikono Ksh Vishnu Anand Virendra Singh Rana Jigni Mishra Manoj Kumar Neelam Upadhyay Charanjit Kaur Source Type: research
Unleashing the bioactive potential of < em > Capsicum chinense < /em > cv Bhut Jolokia: a comparison of microwave- and ultrasound-mediated extraction techniques for lipophilic capsaicin
This study investigated the extraction of capsaicin from Capsicum chinense cv Bhut Jolokia using rice bran oil (RBO) as a 'green solvent' via microwave- and ultrasound-mediated techniques (MME and UME) as an alternative to hazardous organic solvents. Extraction conditions were optimised using MME at 180 and 300 W for 2 and 8 min and UME at 300 and 400 W for 10 and 30 min. The maximum capsaicin yield obtained was 12.47 mg/mL, with a corresponding gamma oryzanol content of 8.46 mg/mL. The peroxide value of the extract (capsaicin-infused RBO) was found to be within permissible limits as per Codex Alimentarius specifications. ...
Source: Natural Product Research - October 3, 2023 Category: Biochemistry Authors: Vikono Ksh Vishnu Anand Virendra Singh Rana Jigni Mishra Manoj Kumar Neelam Upadhyay Charanjit Kaur Source Type: research
3D-Printed Capsaicin-Loaded Injectable Implants for Targeted Delivery in Obese Patients
AAPS PharmSciTech. 2023 Oct 2;24(7):200. doi: 10.1208/s12249-023-02647-z.ABSTRACTDiet-induced obesity and hyperlipidemia are a growing public health concern leading to various metabolic disorders. Capsaicin, a major bioactive compound obtained from natural chili peppers, has demonstrated its numerous beneficial roles in treating obesity and weight loss. Current treatment involves either administration of antiobesity drugs or surgical procedures such as Roux-en-Y-gastric bypass or sleeve gastrectomy, both of which are associated with serious side effects and poor patient acceptance. Capsaicin, a pungent molecule, has low or...
Source: AAPS PharmSciTech - October 2, 2023 Category: Drugs & Pharmacology Authors: Manjusha Annaji Nur Mita Jessica Heard Xuejia Kang Ishwor Poudel Oladiran Fasina Padmamalini Baskaran Sai H S Boddu Amit K Tiwari Pengyu Chen Candace C Lyman R Jayachandra Babu Source Type: research
3D-Printed Capsaicin-Loaded Injectable Implants for Targeted Delivery in Obese Patients
AAPS PharmSciTech. 2023 Oct 2;24(7):200. doi: 10.1208/s12249-023-02647-z.ABSTRACTDiet-induced obesity and hyperlipidemia are a growing public health concern leading to various metabolic disorders. Capsaicin, a major bioactive compound obtained from natural chili peppers, has demonstrated its numerous beneficial roles in treating obesity and weight loss. Current treatment involves either administration of antiobesity drugs or surgical procedures such as Roux-en-Y-gastric bypass or sleeve gastrectomy, both of which are associated with serious side effects and poor patient acceptance. Capsaicin, a pungent molecule, has low or...
Source: AAPS PharmSciTech - October 2, 2023 Category: Drugs & Pharmacology Authors: Manjusha Annaji Nur Mita Jessica Heard Xuejia Kang Ishwor Poudel Oladiran Fasina Padmamalini Baskaran Sai H S Boddu Amit K Tiwari Pengyu Chen Candace C Lyman R Jayachandra Babu Source Type: research
Induction of systemic resistance in Solanum lycopersicum and Capsicum annum seedlings against Fusarium wilt by Streptomyces bioformulations
Environ Sci Pollut Res Int. 2023 Sep 29. doi: 10.1007/s11356-023-29973-w. Online ahead of print.ABSTRACTPlant diseases induced by various phytopathogens pose a significant threat to contemporary agricultural systems around the world. In modern agriculture, the use of pesticides is still a valuable and effective method to control plant diseases. However, agrochemicals are becoming less popular because of the accretion of toxic compounds perilous and potentially hazardous to humans and the environment. Taking into consideration these aspects, the present study was conducted to explore the biocontrol potential of an endophyti...
Source: Environmental Science and Pollution Research International - September 29, 2023 Category: Environmental Health Authors: Sapna Devi Rajesh Kumari Manhas Source Type: research
Induction of systemic resistance in Solanum lycopersicum and Capsicum annum seedlings against Fusarium wilt by Streptomyces bioformulations
Environ Sci Pollut Res Int. 2023 Sep 29. doi: 10.1007/s11356-023-29973-w. Online ahead of print.ABSTRACTPlant diseases induced by various phytopathogens pose a significant threat to contemporary agricultural systems around the world. In modern agriculture, the use of pesticides is still a valuable and effective method to control plant diseases. However, agrochemicals are becoming less popular because of the accretion of toxic compounds perilous and potentially hazardous to humans and the environment. Taking into consideration these aspects, the present study was conducted to explore the biocontrol potential of an endophyti...
Source: Cell Research - September 29, 2023 Category: Cytology Authors: Sapna Devi Rajesh Kumari Manhas Source Type: research
