Viruses, Vol. 16, Pages 687: Development of Attenuated Viruses for Effective Protection against Pepper Veinal Mottle Virus in Tomato Crops
In this study, we performed nitrite-induced mutagenesis of the PVMV tomato isolate Tn to generate attenuated PVMV mutants. PVMV Tn causes necrotic lesions in Chenopodium quinoa leaves and severe mosaic and wilting in Nicotiana benthamiana plants. After nitrite treatment, three attenuated PVMV mutants, m4-8, m10-1, and m10-11, were selected while inducing milder responses to C. quinoa and N. benthamiana with lower accumulation in tomato plants. In greenhouse tests, the three mutants showed different degrees of cross-protection against wild-type PVMV Tn. m4-8 showed the highest protective efficacy against PVMV Tn in N. benth...
Source: Viruses - April 26, 2024 Category: Virology Authors: Guan-Da Wang Chian-Chi Lin Tsung-Chi Chen Tags: Article Source Type: research
Unveiling The Biomedical Applications Of Novel Coumarins Isolated From Capsicum Annuum L. Seeds By A Multivariate Extraction Technique
Chem Biodivers. 2024 Apr 15:e202400581. doi: 10.1002/cbdv.202400581. Online ahead of print.ABSTRACTFor the first time, kinetic thermomagnetic extraction is a novel approach presented in this work. It required the application of four distinct variables: rotation speed (50, 75, and 100 rpm), magnetic field (0.8, 1.2, and 1.6 T), time interval (30, 60, and 90 min), and temperature (45, 55, and 65°C). Numerous phytochemical categories were detected in the 81 crude chloroform extracts of green sweet bell pepper seeds that were collected, according to phytochemical analysis. Nine extracts were discovered to be linked to the cou...
Source: Chemistry and Biodiversity - April 15, 2024 Category: Biochemistry Authors: Areej Hazem Fakri Younes Yasser Fakri Mustafa Source Type: research
Unveiling The Biomedical Applications Of Novel Coumarins Isolated From Capsicum Annuum L. Seeds By A Multivariate Extraction Technique
Chem Biodivers. 2024 Apr 15:e202400581. doi: 10.1002/cbdv.202400581. Online ahead of print.ABSTRACTFor the first time, kinetic thermomagnetic extraction is a novel approach presented in this work. It required the application of four distinct variables: rotation speed (50, 75, and 100 rpm), magnetic field (0.8, 1.2, and 1.6 T), time interval (30, 60, and 90 min), and temperature (45, 55, and 65°C). Numerous phytochemical categories were detected in the 81 crude chloroform extracts of green sweet bell pepper seeds that were collected, according to phytochemical analysis. Nine extracts were discovered to be linked to the cou...
Source: Chemistry and Biodiversity - April 15, 2024 Category: Biochemistry Authors: Areej Hazem Fakri Younes Yasser Fakri Mustafa Source Type: research
Genetic characterization of a locus responsible for low pungency using EMS-induced mutants in Capsicum annuum L
In this study, we characterized two allelic ethyl methanesulfonate-induced mutant lines with extremely low pungency ('221-2-1a' and '1559-1-2h'). These mutants, derived from the pungent Korean landrace 'Yuwolcho,' exhibited lower capsaicinoid content than Yuwolcho but still contained a small amount of capsaicinoid with functional capsaicinoid biosynthetic genes. Genetic crosses between the mutants and Yuwolcho or pungent lines indicated that a single recessive mutation was responsible for the low-pungency phenotype of mutant 221-2-1a; we named the causal locus Pungency 4 (Pun4). To identify Pun4, we combined genome-wide po...
Source: TAG. Theoretical and Applied Genetics - April 12, 2024 Category: Genetics & Stem Cells Authors: Seungki Back Jung-Min Kim Hayoung Choi Joung-Ho Lee Koeun Han Doyeon Hwang Jin-Kyung Kwon Byoung-Cheorl Kang Source Type: research
Genetic characterization of a locus responsible for low pungency using EMS-induced mutants in Capsicum annuum L
In this study, we characterized two allelic ethyl methanesulfonate-induced mutant lines with extremely low pungency ('221-2-1a' and '1559-1-2h'). These mutants, derived from the pungent Korean landrace 'Yuwolcho,' exhibited lower capsaicinoid content than Yuwolcho but still contained a small amount of capsaicinoid with functional capsaicinoid biosynthetic genes. Genetic crosses between the mutants and Yuwolcho or pungent lines indicated that a single recessive mutation was responsible for the low-pungency phenotype of mutant 221-2-1a; we named the causal locus Pungency 4 (Pun4). To identify Pun4, we combined genome-wide po...
Source: TAG. Theoretical and Applied Genetics - April 12, 2024 Category: Genetics & Stem Cells Authors: Seungki Back Jung-Min Kim Hayoung Choi Joung-Ho Lee Koeun Han Doyeon Hwang Jin-Kyung Kwon Byoung-Cheorl Kang Source Type: research
Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsi...
Source: International Journal of Biological Macromolecules - April 10, 2024 Category: Biochemistry Authors: Mar ía Emilia Cano Åsa Lindgren Jennifer Rosendahl Jenny Johansson Alberto Garcia-Martin Miguel Ladero Galan Jos é Kovensky Gary Chinga-Carrasco Source Type: research
Characterization of carboxylated cellulose nanofibrils and oligosaccharides from Kraft pulp fibers and their potential elicitor effect on the gene expression of Capsicum annuum
In this study, cellulose nanofibrils (CNF) gels were obtained by TEMPO-mediated oxidation of unbleached and bleached kraft pulps. The molecular structures were characterized with ESI and MALDI MS. Analysis of the fine sequences was achieved by MS and MS/MS of the water-soluble oligosaccharides obtained by acid hydrolysis of the CNF gels. The analysis revealed the presence of two families: one corresponding to homoglucuronic acid sequences and the other composed by alternating glucose and glucuronic acid units. The CNF gels, alone or with the addition of the water-soluble oligosaccharides, were tested on Chili pepper (Capsi...
Source: International Journal of Biological Macromolecules - April 10, 2024 Category: Biochemistry Authors: Mar ía Emilia Cano Åsa Lindgren Jennifer Rosendahl Jenny Johansson Alberto Garcia-Martin Miguel Ladero Galan Jos é Kovensky Gary Chinga-Carrasco Source Type: research
Identification of Superoxide Dismutase (SOD) Isozymes in Plant Tissues
Methods Mol Biol. 2024;2798:205-212. doi: 10.1007/978-1-0716-3826-2_14.ABSTRACTSuperoxide and hydrogen peroxide are reactive oxygen species (ROS) involved in the oxidation of multiple biological molecules and the signaling processes during plant growth and stress response. Thus, control of ROS is fundamental for cell survival and development, with superoxide dismutase (EC 1.15.1.1, SOD) being one of the main enzymes involved. Different isoforms of SOD catalyze the dismutation of superoxide (O2.-) to hydrogen peroxide (H2O2) and oxygen (O2), such as Mn-SODs, Cu,Zn-SODs, and Fe-SODs. Using non-denaturing polyacrylamide gel e...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Ana Jim énez Sandra Correa Francisca Sevilla Source Type: research
Molecular mapping of the broad bean wilt virus 2 resistance locus bwvr in Capsicum annuum using BSR-seq
Theor Appl Genet. 2024 Apr 8;137(5):97. doi: 10.1007/s00122-024-04603-2.ABSTRACTBulked segregant RNA seq of pools of pepper accessions that are susceptible or resistant to Broad bean wilt virus 2 identifies a gene that might confer resistance to this devastating pathogen. The single-stranded positive-sense RNA virus Broad bean wilt virus 2 (BBWV2) causes substantial damage to pepper (Capsicum annuum) cultivation. Here, we describe mapping the BBWV2 resistance locus bwvr using a F7:8 recombinant inbred line (RIL) population constructed by crossing the BBWV2-resistant pepper accession 'SNU-C' with the susceptible pepper acce...
Source: TAG. Theoretical and Applied Genetics - April 8, 2024 Category: Genetics & Stem Cells Authors: Jung-Min Kim Joung-Ho Lee Se-Ran Park Jin-Kyoung Kwon Na-Young Ro Byoung-Cheorl Kang Source Type: research
Identification of Superoxide Dismutase (SOD) Isozymes in Plant Tissues
Methods Mol Biol. 2024;2798:205-212. doi: 10.1007/978-1-0716-3826-2_14.ABSTRACTSuperoxide and hydrogen peroxide are reactive oxygen species (ROS) involved in the oxidation of multiple biological molecules and the signaling processes during plant growth and stress response. Thus, control of ROS is fundamental for cell survival and development, with superoxide dismutase (EC 1.15.1.1, SOD) being one of the main enzymes involved. Different isoforms of SOD catalyze the dismutation of superoxide (O2.-) to hydrogen peroxide (H2O2) and oxygen (O2), such as Mn-SODs, Cu,Zn-SODs, and Fe-SODs. Using non-denaturing polyacrylamide gel e...
Source: Mol Biol Cell - April 8, 2024 Category: Molecular Biology Authors: Ana Jim énez Sandra Correa Francisca Sevilla Source Type: research
Effects of capsaicin loads on the properties of capsicum leaf protein-based nanocellulose composite films
This study aims to enhance the functionality of conventional protein-based nanocellulose composite films (PNCF) to meet the high demand for natural antimicrobial packaging films. Capsicum leaf protein (CLP) and cellulose nanocrystals (CNCs) extracted from capsicum leaves were used as raw materials. Capsaicin, an essential antibacterial active ingredient in the capsicum plant, was used as an additive. The influence of different capsaicin loads on PNCF physicochemical and material properties was investigated under alkaline conditions. The results show that all film-forming liquids (FFLs) are non-Newtonian fluids with shear t...
Source: International Journal of Biological Macromolecules - March 29, 2024 Category: Biochemistry Authors: Qingyu Qin Bing Gao Xinyan Zhang Lujia Han Swee Leong Sing Xian Liu Source Type: research
< em > Capsicum annuum < /em > Oleoresin Nanoemulgel - Design Characterization and < em > In vitro < /em > Investigation of Anticancer and Antimicrobial Activities
CONCLUSION: C. annuum oleoresin and its nanoemulgel can be considered valuable sources for the discovery of new antibacterial, antifungal, and anticancer compounds in the pharmaceutical industry, especially due to their potent activity against various cancer cell lines as well as bacterial and fungal strains.PMID:38532324 | DOI:10.2174/0113816128283684231220062019 (Source: Current Pharmaceutical Design)
Source: Current Pharmaceutical Design - March 27, 2024 Category: Drugs & Pharmacology Authors: Ahmad M Eid Hiba Natsheh Linda Issa Majdulin Zoabi Monia Amer Effat Mahamid Ahmed Mousa Source Type: research
< em > Capsicum annuum < /em > Oleoresin Nanoemulgel - Design Characterization and < em > In vitro < /em > Investigation of Anticancer and Antimicrobial Activities
CONCLUSION: C. annuum oleoresin and its nanoemulgel can be considered valuable sources for the discovery of new antibacterial, antifungal, and anticancer compounds in the pharmaceutical industry, especially due to their potent activity against various cancer cell lines as well as bacterial and fungal strains.PMID:38532324 | DOI:10.2174/0113816128283684231220062019 (Source: Current Pharmaceutical Design)
Source: Current Pharmaceutical Design - March 27, 2024 Category: Drugs & Pharmacology Authors: Ahmad M Eid Hiba Natsheh Linda Issa Majdulin Zoabi Monia Amer Effat Mahamid Ahmed Mousa Source Type: research
Polyvinyl alcohol/kappa-carrageenan-based package film with simultaneous incorporation of ferric ion and polyphenols from Capsicum annuum leaves for fruit shelf-life extension
Int J Biol Macromol. 2024 Mar 22:131002. doi: 10.1016/j.ijbiomac.2024.131002. Online ahead of print.ABSTRACTBio-based food packaging materials have elicited growing interests due to their great degradability, high safety and active biofunctions. In this work, by simultaneously introducing the polyphenolic extracts from Capsicum annuum leaves and ferric ion (Fe3+) into the Polyvinyl alcohol/kappa-carrageenan (PVA/κ-carrageenan)-based film-forming matrix, an active package film was developed, with the purpose to improve the food shelf life. The experimental results indicated that the existence of Fe3+ can not only improve t...
Source: International Journal of Biological Macromolecules - March 24, 2024 Category: Biochemistry Authors: Xiaojun Li Yiyuan Fan Juan Guo Ran Li Zeqi Liu Yarui Hou Zhican Qu Qingye Liu Source Type: research
Integrated transcriptome and metabolome analysis reveals anthocyanin biosynthesis mechanisms in pepper (Capsicum annuum L.) leaves under continuous blue light irradiation
Different metabolic compounds give pepper leaves and fruits their diverse colors. Anthocyanin accumulation is the main cause of the purple color of pepper leaves. The light environment is a critical factor aff... (Source: BMC Plant Biology)
Source: BMC Plant Biology - March 23, 2024 Category: Biology Authors: Yao Zhou, Weisheng Wu, Ying Sun, Yiyu Shen, Lianzhen Mao, Yunhua Dai, Bozhi Yang and Zhoubin Liu Tags: Research Source Type: research
