KCTD1 is a new modulator of the KCASH family of Hedgehog suppressors
Neoplasia. 2023 Aug 17;43:100926. doi: 10.1016/j.neo.2023.100926. Online ahead of print.ABSTRACTThe Sonic Hedgehog (Hh) signal transduction pathway plays a critical role in many developmental processes and, when deregulated, may contribute to several cancers, including basal cell carcinoma, medulloblastoma, colorectal, prostate, and pancreatic cancer. In recent years, several Hh inhibitors have been developed, mainly acting on the Smo receptor. However, drug resistance due to Smo mutations or non-canonical Hh pathway activation highlights the need to identify further mechanisms of Hh pathway modulation. Among these, deacet...
Source: Neoplasia - August 19, 2023 Category: Cancer & Oncology Authors: A Di Fiore S Bellardinelli L Pirone R Russo A Angrisani G Terriaca M Bowen F Bordin Z M Besharat G Canettieri F Fabretti S Di Gaetano L Di Marcotullio E Pedone M Moretti E De Smaele Source Type: research
KCTD1 is a new modulator of the KCASH family of Hedgehog suppressors
Neoplasia. 2023 Aug 17;43:100926. doi: 10.1016/j.neo.2023.100926. Online ahead of print.ABSTRACTThe Sonic Hedgehog (Hh) signal transduction pathway plays a critical role in many developmental processes and, when deregulated, may contribute to several cancers, including basal cell carcinoma, medulloblastoma, colorectal, prostate, and pancreatic cancer. In recent years, several Hh inhibitors have been developed, mainly acting on the Smo receptor. However, drug resistance due to Smo mutations or non-canonical Hh pathway activation highlights the need to identify further mechanisms of Hh pathway modulation. Among these, deacet...
Source: Neoplasia - August 19, 2023 Category: Cancer & Oncology Authors: A Di Fiore S Bellardinelli L Pirone R Russo A Angrisani G Terriaca M Bowen F Bordin Z M Besharat G Canettieri F Fabretti S Di Gaetano L Di Marcotullio E Pedone M Moretti E De Smaele Source Type: research
Oncolytic virus-driven immune remodeling revealed in mouse medulloblastomas at single cell resolution
Mol Ther Oncolytics. 2023 Jul 19;30:39-55. doi: 10.1016/j.omto.2023.07.006. eCollection 2023 Sep 21.ABSTRACTOncolytic viruses, modified for tumor-restricted infection, are a promising cancer immunotherapeutic, yet much remains to be understood about factors driving their activity and outcome in the tumor microenvironment. Here, we report that oncolytic herpes simplex virus C134, previously found to exert T cell-dependent efficacy in mouse models of glioblastoma, exerts T cell-independent efficacy in mouse models of medulloblastoma, indicating this oncolytic virus uses different mechanisms in different tumors. We investigat...
Source: Herpes - August 16, 2023 Category: Infectious Diseases Authors: Jack Hedberg Adam Studebaker Luke Smith Chun-Yu Chen Jesse J Westfall Maren Cam Amy Gross Ilse Hernandez-Aguirre Alexia Martin Doyeon Kim Ravi Dhital Yeaseul Kim Ryan D Roberts Timothy P Cripe Elaine R Mardis Kevin A Cassady Jeffrey Leonard Katherine E Mi Source Type: research
Oncolytic virus-driven immune remodeling revealed in mouse medulloblastomas at single cell resolution
Mol Ther Oncolytics. 2023 Jul 19;30:39-55. doi: 10.1016/j.omto.2023.07.006. eCollection 2023 Sep 21.ABSTRACTOncolytic viruses, modified for tumor-restricted infection, are a promising cancer immunotherapeutic, yet much remains to be understood about factors driving their activity and outcome in the tumor microenvironment. Here, we report that oncolytic herpes simplex virus C134, previously found to exert T cell-dependent efficacy in mouse models of glioblastoma, exerts T cell-independent efficacy in mouse models of medulloblastoma, indicating this oncolytic virus uses different mechanisms in different tumors. We investigat...
Source: Herpes - August 16, 2023 Category: Infectious Diseases Authors: Jack Hedberg Adam Studebaker Luke Smith Chun-Yu Chen Jesse J Westfall Maren Cam Amy Gross Ilse Hernandez-Aguirre Alexia Martin Doyeon Kim Ravi Dhital Yeaseul Kim Ryan D Roberts Timothy P Cripe Elaine R Mardis Kevin A Cassady Jeffrey Leonard Katherine E Mi Source Type: research
Hedgehog target genes regulate lipid metabolism to drive basal cell carcinoma and medulloblastoma
Res Sq. 2023 Aug 3:rs.3.rs-3058335. doi: 10.21203/rs.3.rs-3058335/v1. Preprint.ABSTRACTHedgehog (Hh) signaling is essential for development, homeostasis, and regeneration 1 . Misactivation of the Hh pathway underlies medulloblastoma, the most common malignant brain tumor in children, and basal cell carcinoma (BCC), the most common cancer in the United States 2 . Primary cilia regulate Hh signal transduction 3 , but target genes that drive cell fate decisions in response to ciliary ligands or oncogenic Hh signaling are incompletely understood. Here we define the Hh gene expression program using RNA sequencing of cultured ce...
Source: Cell Research - August 14, 2023 Category: Cytology Authors: Vikas Daggubati Akshara Vykunta Abrar Choudhury Zulekha Qadeer Kanish Mirchia Olivier Saulnier Naomi Zakimi Kelly Hines Michael Paul Linyu Wang Natalia Jura Libin Xu Jeremy Reiter Michael Taylor William Weiss David Raleigh Source Type: research
Tumorsphere Formation Assay: A Cancer Stem-Like Cell Characterization in Pediatric Brain Cancer Medulloblastoma
We describe here the detailed methodology for the generation and propagation of spheres generated from pediatric brain tumor medulloblastoma (MB) cells. As signal transducer and activator of transcription (STAT3) is known to play an important role in maintaining cancer stem cell properties, we accessed the effect of depleting or inhibiting STAT3 on MB-sphere sizes, numbers, and integrity. This may serve as a promising platform for screening potential anti-CSC agents and small-molecule inhibitors.PMID:37574488 | DOI:10.1007/978-1-0716-3373-1_17 (Source: Mol Biol Cell)
Source: Mol Biol Cell - August 13, 2023 Category: Molecular Biology Authors: Sutapa Ray Source Type: research
Retraction Statement: Inhibition of GPR137 suppresses proliferation of medulloblastoma cells in vitro
(Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - August 10, 2023 Category: Biochemistry Tags: RETRACTION Source Type: research
Cytotoxicity of poly-guanidine in medulloblastoma cell lines
SummaryMedulloblastoma (MB) is the most common pediatric brain tumor. The therapy frequently causes serious side effects, and new selective therapies are needed. MB expresses hyper sialylation, a possible target for selective therapy. The cytotoxic efficacy of a poly guanidine conjugate (GuaDex) incubated with medulloblastoma cell cultures (DAOY and MB-LU-181) was investigated. The cells were incubated with 0.05–8 µM GuaDex from 15 min to 72 h. A fluorometric cytotoxicity assay (FMCA) measured the cytotoxicity. Labeled Gu aDex was used to study tumor cell interaction. FITC-labelSambucus nigra confirmed high express...
Source: Investigational New Drugs - August 9, 2023 Category: Drugs & Pharmacology Source Type: research
Integrated analysis of multiple methods reveals characteristics of the immune microenvironment in medulloblastoma
AbstractTo explore the characteristics of the immune microenvironment (IME) of medulloblastoma (MB) by four methods: flow cytometry (FCM), immunohistochemical (IHC), bulk RNA expression and single cell RNA sequencing (scRNA-seq), we collected the intraoperative specimens of MB, ependymoma (EPN), high-grade glioma (HGG), and low-grade glioma (LGG) to make a cross-cancer comparison. The specimens were subjected to FCM and IHC respectively, and deconvolution from bulk RNA expression data and scRNA-seq analysis were performed in MB from the GEO database. FCM and IHC analysis found that the proportion of lymphocytes (LC) and T ...
Source: Brain Tumor Pathology - August 9, 2023 Category: Neurology Source Type: research
Cancers, Vol. 15, Pages 4015: Deciphering Machine Learning Decisions to Distinguish between Posterior Fossa Tumor Types Using MRI Features: What Do the Data Tell Us?
Keserci
Machine learning (ML) models have become capable of making critical decisions on our behalf. Nevertheless, due to complexity of these models, interpreting their decisions can be challenging, and humans cannot always control them. This paper provides explanations of decisions made by ML models in diagnosing four types of posterior fossa tumors: medulloblastoma, ependymoma, pilocytic astrocytoma, and brainstem glioma. The proposed methodology involves data analysis using kernel density estimations with Gaussian distributions to examine individual MRI features, conducting an analysis on the relationships between t...
Source: Cancers - August 8, 2023 Category: Cancer & Oncology Authors: Toygar Tanyel Chandran Nadarajan Nguyen Minh Duc Bilgin Keserci Tags: Article Source Type: research
The Association Between Socioeconomic Factors at Diagnosis and Survival in Medulloblastoma: A Propensity Score-Matched Analysis and Population-Based Study
ConclusionsThe unique features of MB have provided a scenario for analysis of the impact of racial, ethnic, gender, and socioeconomic factors. The current findings have important public health implications for achieving the goal of a healthy population. Given the known morbidity rates, long-term psychological, financial and medical burdens that these children and their families must bear, it is critical to identify and address these gaps. (Source: Journal of Racial and Ethnic Health Disparities)
Source: Journal of Racial and Ethnic Health Disparities - August 7, 2023 Category: International Medicine & Public Health Source Type: research
Smurf1 and Smurf2 mediated polyubiquitination and degradation of RNF220 suppresses Shh-group medulloblastoma
Cell Death & Disease, Published online: 03 August 2023; doi:10.1038/s41419-023-06025-2Smurf1 and Smurf2 mediated polyubiquitination and degradation of RNF220 suppresses Shh-group medulloblastoma (Source: Cell death and disease)
Source: Cell death and disease - August 3, 2023 Category: Internal Medicine Authors: Yuwei Li Huishan Wang Bin Sun Guifeng Su Yu Cang Ling Zhao Shuhua Zhao Yan Li Bingyu Mao Pengcheng Ma Source Type: research
Predictors of postoperative complications and functional outcomes in pediatric patients with surgically treated fourth ventricle tumors
ConclusionThe overall rate of postoperative complications remains high due to the eloquent localization. Older patients (> 7 years) have been found to have better outcomes and prognosis. Achieving GTR whenever feasible and safe has been shown to be critical for tumor recurrence. CM was more common in patients with medulloblastoma and in patients with tumors extending through the foramen of Luschka. The telovelar app roach uses a safe and anatomically sparing corridor; however, it has not been associated with a lower incidence of CM and neurological sequelae in our series, showing that each case should be assessed on...
Source: Acta Neurochirurgica - August 3, 2023 Category: Neurosurgery Source Type: research
