PUMC-MB1 is a novel group 3 medulloblastoma preclinical model, sensitive to PI3K/mTOR dual inhibitor
ConclusionsPUMC-MB1, a novel Group 3 (Non-WNT/Non-SHH) MB cell line, is comprehensively characterized for its growth, pathology, and molecular characteristics. Notably, dactolisib demonstrated potent anti-proliferative effects with minimal toxicity, promising a potential therapeutic avenue. PUMC-MB1 could serve as a valuable tool for unraveling MB mechanisms and innovative treatment strategies. (Source: Journal of Neuro-Oncology)
Source: Journal of Neuro-Oncology - April 25, 2024 Category: Cancer & Oncology Source Type: research
A Prognostic Methylation-Driven Two-Gene Signature in Medulloblastoma
In this study, we used six datasets totaling 1679 samples including RNA gene expression and DNA methylation data from primary MB as well as control samples from healthy cerebellum. We identified methylation-driven genes (MDGs) in MB, genes whose expression is correlated with their methylation. We employed LASSO regression, incorporating the MDGs as a parameter to develop the prognostic model. Through this approach, we deri ved a two-gene signature (GS-2) of candidate prognostic biomarkers for MB (CEMIP andNCBP3). Using a risk score model, we confirmed the GS-2 impact on overall survival (OS) with Kaplan-Meier analysis. We ...
Source: Journal of Molecular Neuroscience - April 25, 2024 Category: Neuroscience Source Type: research
Percutaneous Ventriculoatrial Shunting as a Salvage Method in the Pediatric Hydrocephalus Patients with Peritoneal Problems
CONCLUSION: Percutaneous ventriculoatrial shunting under the guidance of intraoperative fluoroscopy and ultrasound is a safe, effective, and easy alternative in patients with peritoneal complications and a history of multiple operations.PMID:38650557 | DOI:10.5137/1019-5149.JTN.43472-23.2 (Source: Turkish Neurosurgery)
Source: Turkish Neurosurgery - April 23, 2024 Category: Neurosurgery Authors: Kadir Oktay Sinan Sozutok Umur Anil Pehlivan Dogu Cihan Yildirim Mevlana Akbaba Kerem Mazhar Ozsoy Nuri Eralp Cetinalp Source Type: research
Percutaneous Ventriculoatrial Shunting as a Salvage Method in the Pediatric Hydrocephalus Patients with Peritoneal Problems
CONCLUSION: Percutaneous ventriculoatrial shunting under the guidance of intraoperative fluoroscopy and ultrasound is a safe, effective, and easy alternative in patients with peritoneal complications and a history of multiple operations.PMID:38650557 | DOI:10.5137/1019-5149.JTN.43472-23.2 (Source: Turkish Neurosurgery)
Source: Turkish Neurosurgery - April 23, 2024 Category: Neurosurgery Authors: Kadir Oktay Sinan Sozutok Umur Anil Pehlivan Dogu Cihan Yildirim Mevlana Akbaba Kerem Mazhar Ozsoy Nuri Eralp Cetinalp Source Type: research
GSE240627 SMARCA4 loss and mutated β-catenin induce proliferative lesions in the murine embryonic cerebellum
Contributors : Carolin G öbel ; Melanie Schoof ; Dörthe Holdhof ; Michael Spohn ; Ulrich SchüllerSeries Type : Methylation profiling by arrayOrganism : Mus musculusAlmost all medulloblastomas (MB) of the Wingless/Int-1 (WNT) subgroup are characterized by hotspot mutations in CTNNB1, and mouse models have convincingly demonstrated the tumor-initiating role of these mutations. Additional alterations in SMARCA4 are detected in around 20 % of WNT MB but their functional role is mostly unknown. We therefore amended previously described Blbp-cre::Ctnnb1(ex3)fl/wt mice by the introduction of a floxed Smarca4 allele. In co...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Methylation profiling by array Mus musculus Source Type: research
GSE241018 SMARCA4 loss and mutated β-catenin induce proliferative lesions in the murine embryonic cerebellum [scRNA-seq]
Contributors : Carolin G öbel ; Melanie Schoof ; Dörthe Holdhof ; Michael Spohn ; Ulrich SchüllerSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusAlmost all medulloblastomas (MB) of the Wingless/Int-1 (WNT) type are characterized by hotspot mutations in CTNNB1, and mouse models have convincingly demonstrated the tumor-initiating role of these mutations. Additional alterations in SMARCA4 are detected in around 20% of WNT MB, but their functional role is mostly unknown. We therefore amended previously described Blbp cre::Ctnnb1(ex3)fl/wt mice by the introduction of a floxed Smarc...
Source: GEO: Gene Expression Omnibus - April 17, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Functional expression of the proton sensors ASIC1a, TMEM206, and OGR1 together with BK < sub > Ca < /sub > channels is associated with cell volume changes and cell death under strongly acidic conditions in DAOY medulloblastoma cells
In this study, using RT-qPCR, whole-cell patch clamp, and Ca2+-imaging, we uncovered several ion channels and a G protein-coupled receptor, which were regulated directly or indirectly by low extracellular pH in DAOY and UW228 medulloblastoma cells. Acidification directly activated acid-sensing ion channel 1a (ASIC1a), the proton-activated Cl- channel (PAC, ASOR, or TMEM206), and the proton-activated G protein-coupled receptor OGR1. The resulting Ca2+ signal secondarily activated the large conductance calcium-activated potassium channel (BKCa). Our analyses uncover a complex relationship of these transmembrane proteins in D...
Source: Pflugers Archiv : European Journal of Physiology - April 16, 2024 Category: Physiology Authors: Karolos-Philippos Pissas Stefan Gr ünder Yuemin Tian Source Type: research
Functional expression of the proton sensors ASIC1a, TMEM206, and OGR1 together with BK < sub > Ca < /sub > channels is associated with cell volume changes and cell death under strongly acidic conditions in DAOY medulloblastoma cells
In this study, using RT-qPCR, whole-cell patch clamp, and Ca2+-imaging, we uncovered several ion channels and a G protein-coupled receptor, which were regulated directly or indirectly by low extracellular pH in DAOY and UW228 medulloblastoma cells. Acidification directly activated acid-sensing ion channel 1a (ASIC1a), the proton-activated Cl- channel (PAC, ASOR, or TMEM206), and the proton-activated G protein-coupled receptor OGR1. The resulting Ca2+ signal secondarily activated the large conductance calcium-activated potassium channel (BKCa). Our analyses uncover a complex relationship of these transmembrane proteins in D...
Source: Pflugers Archiv : European Journal of Physiology - April 16, 2024 Category: Physiology Authors: Karolos-Philippos Pissas Stefan Gr ünder Yuemin Tian Source Type: research
Comparing the effects of irradiation with protons or photons on neonatal mouse brain: Apoptosis, oncogenesis and hippocampal alterations
CONCLUSION: In-vivo experiments with radiosensitive mouse models improve our mechanistic understanding of the dependence of brain damage on radiation quality, thus having important implications in translational research.PMID:38614282 | DOI:10.1016/j.radonc.2024.110267 (Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology)
Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology - April 13, 2024 Category: Radiology Authors: Daniela Giovannini Francesca Antonelli Arianna Casciati Cinzia De Angelis Maria Denise Astorino Giulia Bazzano Emiliano Fratini Alessandro Ampollini Monia Vadrucci Evaristo Cisbani Paolo Nenzi Luigi Picardi Anna Saran Carmela Marino Mariateresa Mancuso Co Source Type: research
Comparing the effects of irradiation with protons or photons on neonatal mouse brain: Apoptosis, oncogenesis and hippocampal alterations
CONCLUSION: In-vivo experiments with radiosensitive mouse models improve our mechanistic understanding of the dependence of brain damage on radiation quality, thus having important implications in translational research.PMID:38614282 | DOI:10.1016/j.radonc.2024.110267 (Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology)
Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology - April 13, 2024 Category: Radiology Authors: Daniela Giovannini Francesca Antonelli Arianna Casciati Cinzia De Angelis Maria Denise Astorino Giulia Bazzano Emiliano Fratini Alessandro Ampollini Monia Vadrucci Evaristo Cisbani Paolo Nenzi Luigi Picardi Anna Saran Carmela Marino Mariateresa Mancuso Co Source Type: research
Comparing the effects of irradiation with protons or photons on neonatal mouse brain: Apoptosis, oncogenesis and hippocampal alterations
CONCLUSION: In-vivo experiments with radiosensitive mouse models improve our mechanistic understanding of the dependence of brain damage on radiation quality, thus having important implications in translational research.PMID:38614282 | DOI:10.1016/j.radonc.2024.110267 (Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology)
Source: Radiotherapy and Oncology : journal of the European Society for Therapeutic Radiology and Oncology - April 13, 2024 Category: Radiology Authors: Daniela Giovannini Francesca Antonelli Arianna Casciati Cinzia De Angelis Maria Denise Astorino Giulia Bazzano Emiliano Fratini Alessandro Ampollini Monia Vadrucci Evaristo Cisbani Paolo Nenzi Luigi Picardi Anna Saran Carmela Marino Mariateresa Mancuso Co Source Type: research
Cancers, Vol. 16, Pages 1474: Human-Level Differentiation of Medulloblastoma from Pilocytic Astrocytoma: A Real-World Multicenter Pilot Study
Johann
Hau
Frühwald
Medulloblastoma and pilocytic astrocytoma are the two most common pediatric brain tumors with overlapping imaging features. In this proof-of-concept study, we investigated using a deep learning classifier trained on a multicenter data set to differentiate these tumor types. We developed a patch-based 3D-DenseNet classifier, utilizing automated tumor segmentation. Given the heterogeneity of imaging data (and available sequences), we used all individually available preoperative imaging sequences to make the model robust to varying input. We compared the classifier to diagnostic assessments by f...
Source: Cancers - April 11, 2024 Category: Cancer & Oncology Authors: Wiestler Bison Behrens T üchert Metz Griessmair Jakob Schlegel Binder von Luettichau Metzler Johann Hau Fr ühwald Tags: Article Source Type: research
Comparing the effects of irradiation with protons or photons on neonatal mouse brain: Apoptosis, oncogenesis and hippocampal alterations
Proton therapy offers a favorable depth-dose deposition with the advantage of sparing healthy tissues, therefore potentially reducing radiation side effects. Proton therapy is commonly used to treat a range of tumors including central nervous tumors, such as medulloblastoma (MB) [1]. (Source: Radiotherapy and Oncology)
Source: Radiotherapy and Oncology - April 10, 2024 Category: Radiology Authors: Daniela Giovannini, Francesca Antonelli, Arianna Casciati, Cinzia De Angelis, Maria Denise Astorino, Giulia Bazzano, Emiliano Fratini, Alessandro Ampollini, Monia Vadrucci, Evaristo Cisbani, Paolo Nenzi, Luigi Picardi, Anna Saran, Carmela Marino, Mariater Tags: Original Article Source Type: research
Pediatric-Like Brain Tumors in Adults
Adv Tech Stand Neurosurg. 2024;50:147-183. doi: 10.1007/978-3-031-53578-9_5.ABSTRACTPediatric brain tumors are different to those found in adults in pathological type, anatomical site, molecular signature, and probable tumor drivers. Although these tumors usually occur in childhood, they also rarely present in adult patients, either as a de novo diagnosis or as a delayed recurrence of a pediatric tumor in the setting of a patient that has transitioned into adult services.Due to the rarity of pediatric-like tumors in adults, the literature on these tumor types in adults is often limited to small case series, and treatment d...
Source: Advances and Technical Standards in Neurosurgery - April 9, 2024 Category: Neurosurgery Authors: Sandra Fernandes Dias Oliver Richards Martin Elliot Paul Chumas Source Type: research
