Anti-Inflammatory Effect of Local Anaesthetic Ropivacaine in Lipopolysaccharide-Stimulated RAW264.7 Macrophages
The present manuscript intended to investigate the anti-inflammatory effect of ropivacaine on lipopolysaccharide-induced inflammation in RAW 264.7 macrophages. Results suggested that ropivacaine causes significant inhibition of generation of nitric oxide (NO), prostaglandin E2, tumor necrosis factor (TNF)- α, interleukin (IL)-6, and IL-1β, as well as expression of their synthesizing enzymes, inducible NO synthase, and cyclooxygenase-2. Moreover, ropivacaine causes inhibition of mitogen-activated protein kinases as well as nuclear factor-kappa B signaling pathway and apoptosis in RAW 264.7 cells. Bas ed on the results, it...
Source: Pharmacology - January 28, 2019 Category: Drugs & Pharmacology Source Type: research
Anti-Inflammatory and Hepatoprotective Effects of < b > < i > Ganoderma lucidum < /i > < /b > Polysaccharides against Carbon Tetrachloride-Induced Liver Injury in Kunming Mice
Background:Ganoderma lucidum Polysaccharides (GLPS) were found to possess various pharmacological properties including anti-inflammatory and hepatoprotective activities. However, the effect and possible mechanism of GLPS treatment on liver injury have not yet been reported. Therefore, this study aimed to explore the potential anti-inflammatory and hepatoprotective effects and possible mechanism of GLPS in carbon tetrachloride (CCl4)-induced acute liver injury mice.Summary: GLPS significantly reduced the activation of NLRP3 inflammasome and improved liver function in liver injury mice. It significantly inhibited CCl4-induce...
Source: Pharmacology - January 27, 2019 Category: Drugs & Pharmacology Source Type: research
Blockade of Serotonin 2C Receptors with SB-242084 Moderates Reduced Locomotor Activity and Rearing by Cannabinoid 1 Receptor Antagonist AM-251
The endocannabinoid and serotonin (5-HT) systems have key roles in the regulation of several physiological functions such as motor activity and food intake but also in the development of psychiatric disorders. Here we tested the hypothesis, whether blockade of serotonin 2C (5-HT2C) receptors prevents the reduced locomotor activity and other behavioral effects caused by a cannabinoid 1 (CB1) receptor antagonist. As a pretreatment, we administered SB-242084 (1 mg/kg, ip.), a 5-HT2C receptor antagonist or vehicle (VEH) followed by the treatment with AM-251 (5 or 10 mg/kg, ip.), a CB1 receptor antagonist or VEH. The effects of...
Source: Pharmacology - January 27, 2019 Category: Drugs & Pharmacology Source Type: research
Tanshinone IIA Ameliorate Coxsackie Virus B3-Induced Viral Myocarditis through the Inhibition of Inflammation and Modulation T Helper 1/T Helper 2 Balance in Mice
To investigate the effect of Tanshinone IIA (TSA) on viral myocarditis (VMC). VMC animal model was established using BALB/c mice by intraperitoneally injecting Coxsackie virus B3 (CVB3). The mice were randomly divided into control group, model group, and TSA group. We detected the survival rate, the heart weight to body weight (HW/BW) ratio and hemodynamic and cardiac function parameters. The pathological features of VMC were measured through Hamp;E staining. The expression of serum enzyme, inflammatory cytokines, and T helper (Th)1/Th2 markers was also investigated. TSA remarkably alleviated CVB3-caused myocardial injury,...
Source: Pharmacology - January 2, 2019 Category: Drugs & Pharmacology Source Type: research
Effective Ibuprofen Desensitization in a Patient with Myopericarditis
We report an efficacious desensitization procedure for ibuprofen in urticaria/angioedema patient with pericarditis and myocarditis associated.Pharmacology 2019;103:111 –113 (Source: Pharmacology)
Source: Pharmacology - December 13, 2018 Category: Drugs & Pharmacology Source Type: research
Ameliorative Effect of < b > < i > Withaferin < /i > < /b > < b > < i > A < /i > < /b > on Ageing-Mediated Impairment in the Dopamine System and Its Associated Behavior of Wistar Albino Rat
This study is the first report that evidently determines the neuro-protective efficacy of WA on dopaminergic system of SN and ST in aged r ats.Pharmacology 2019;103:114 –119 (Source: Pharmacology)
Source: Pharmacology - December 13, 2018 Category: Drugs & Pharmacology Source Type: research
In vitro Inhibitory Effects of Isofraxidin on Human Liver Cytochrome P450 Enzymes
In this study, the inhibitory effects of isofraxidin on the 8 human liver CYP isoforms (i.e., 1A2, 3A4, 2A6, 2E1, 2D6, 2C9, 2C19, and 2C8) were investigated in vitro using human liver microsomes. The results showed that isofraxidin inhibited the activity of CYP1A2, 3A4, and 2E1, withIC50 values of 23.01, 15.49, and 15.98 µmol/L, respectively, but that other CYP isoforms were not affected. Enzyme kinetic studies showed that isofraxidin was not only a noncompetitive inhibitor of CYP3A4 but also a competitive inhibitor of CYP1A2 and 2E1, withKi values of 7.91, 10.14, and 9.30 µmol/L, respectively. In addition, isofraxidin i...
Source: Pharmacology - December 13, 2018 Category: Drugs & Pharmacology Source Type: research
Expression of Matrix Metalloproteinase-2, Tissue Inhibitor of Matrix Metalloproteinase-2 and CD147 in the Traditional Chinese Medicine “Compound T11” for Treatment of Chronic Liver Injury
Conclusions: Treatment of chronic liver injury by TCM Compound T11 may be associated with changes to the expression of MMP-2 and CD147, and the inhibition of TIMP-2 expression.Pharmacology 2019;103:128 –135 (Source: Pharmacology)
Source: Pharmacology - December 13, 2018 Category: Drugs & Pharmacology Source Type: research
Taxifolin Inhibits Receptor Activator of NF- κB Ligand-Induced Osteoclastogenesis of Human Bone Marrow-Derived Macrophages in vitro and Prevents Lipopolysaccharide-Induced Bone Loss in vivo
In conclusion, the results suggested that taxifolin has a therapeutic potential for osteoclastogenesis-related diseases such as osteoporosis, osteolysis, and rheumatoid arthritis.Pharmacology 2019;103:101 –109 (Source: Pharmacology)
Source: Pharmacology - December 6, 2018 Category: Drugs & Pharmacology Source Type: research
Involvement of the cAMP-Dependent Pathway in Dextromethorphan-Induced Inhibition of Spontaneous Glutamate Transmission in the Nucleus Tractus Solitarius Neurons of Guinea Pigs
Dextromethorphan (DEX) presynaptically decreases glutamatergic transmission in second-order neurons of the nucleus tractus solitarius (TS). To clarify the inhibitory mechanism of DEX, the present study examined the interaction of DEX with cAMP. The effects of DEX on miniature and TS-evoked excitatory postsynaptic currents (mEPSCs and eEPSCs) were recorded under activation of the cAMP-dependent pathway using the brainstem slices. An increase in cAMP by forskolin counteracted the inhibitory effect of DEX on mEPSCs. Eight-Bromo-cAMP and N-ethylmaleimide also attenuated the DEX effect. However, forskolin had negligible effects...
Source: Pharmacology - December 5, 2018 Category: Drugs & Pharmacology Source Type: research
Mechanisms of Etomidate-Mediated Decrease of Spontaneous Spike Activity of Mouse Cerebellar Purkinje Cells in vivo
In this study, we investigated the effects of etomidate on the spontaneous simple spike (SS) activity of PCs in urethane-anesthetized mice by cell-attached recording and pharmacological methods. Cerebellar surface application of etomidate (50 μmol\L) reduced the SS firing rate in a concentration-dependent manner (IC50: 43.4 µmol\L). Application of either a γ-aminobutyric acid type A (GABAA) receptor antagonist, SR95531 (20 μmol\L) or a glycine receptor antagonist strychnine (10 μmol\L) significantly attenuated but not abolished the etomidate-induced decrease in PC SS firing rate. However, co-application of SR95531 (20...
Source: Pharmacology - December 5, 2018 Category: Drugs & Pharmacology Source Type: research
Tauroursodeoxycholic Acid Ameliorates Lipopolysaccharide-Induced Depression Like Behavior in Mice via the Inhibition of Neuroinflammation and Oxido-Nitrosative Stress
In this study, we investigated the effect of TUDCA on lipopolysaccharide (LPS)-induced depression-like behavior, neuroinflammation, and oxido-nitrosative stress in mice. Results showed that TUDCA pretreatment (once daily for 7 consecutive days) at the dosage of 200 and 400 mg/kg, but not 100 mg/kg, markedly attenuated LPS (0.83 mg/kg)-induced behavioral abnormalities in the tail suspension test, forced swim test, and sucrose preference test. Further analysis showed that the TUDCA pretreatment (200, 400 mg/kg) not only inhibited the production of proinflammatory c ytokines induced by LPS stimulation, such as interleuki...
Source: Pharmacology - December 5, 2018 Category: Drugs & Pharmacology Source Type: research
ErbB4 Receptors in the Medial Habenula Regulate Contextual Fear Memory
In this study, western blotting and quantitative real-time polymerase chain reaction were used to assess the protein and mRNA levels of ErbB4 in the process of contextual fear conditioning. A pharmacological approach was used to block and stimulate the ErbB4 receptor. Contextual fear conditioning tests induced a significant increase on the expression of ErbB4 at various times in the Hb and the MHb. Moreover, the blockade and stimulation of MHb ErbB4 receptors did not affect the fear formation but impaired and improved the contextual-dependent fear expression. Furthermore, in vitro electrophysiological recordings showed tha...
Source: Pharmacology - December 4, 2018 Category: Drugs & Pharmacology Source Type: research
Acknowledgement to Referees
Pharmacology 2018;102:347 –348 (Source: Pharmacology)
Source: Pharmacology - November 20, 2018 Category: Drugs & Pharmacology Source Type: research
Contents Vol. 102, 2018
Pharmacology 2018;102:I –IV (Source: Pharmacology)
Source: Pharmacology - November 20, 2018 Category: Drugs & Pharmacology Source Type: research
