Interlaboratory evaluation of quality control methods for circulating cell-free DNA extraction
In this study we evaluated the application of nucleic acid "spike-in" control materials to aid quality control (QC) and standardisation of cfDNA isolation for use in in vitro diagnostic assays. We describe an approach for the design and characterisation of in-process QC materials, illustrating it with a spike-in material containing an exogenous Arabidopsis sequence and DNA fragments approximating to ccfDNA and genomic DNA lengths. Protocols for inclusion of the spike-in material in plasma ccfDNA extraction and quantification of its recovery by digital PCR (dPCR) were assessed for their suitability for process QC in an inte...
Source: New Biotechnology - September 20, 2023 Category: Biotechnology Authors: Alison Devonshire Gerwyn Jones Ana Fernandez Gonzalez Olga Kofanova Johanna Trouet Pamela Pinzani Stefania Gelmini Serena Bonin Carole Foy Source Type: research
Interlaboratory evaluation of quality control methods for circulating cell-free DNA extraction
In this study we evaluated the application of nucleic acid "spike-in" control materials to aid quality control (QC) and standardisation of cfDNA isolation for use in in vitro diagnostic assays. We describe an approach for the design and characterisation of in-process QC materials, illustrating it with a spike-in material containing an exogenous Arabidopsis sequence and DNA fragments approximating to ccfDNA and genomic DNA lengths. Protocols for inclusion of the spike-in material in plasma ccfDNA extraction and quantification of its recovery by digital PCR (dPCR) were assessed for their suitability for process QC in an inte...
Source: New Biotechnology - September 20, 2023 Category: Biotechnology Authors: Alison Devonshire Gerwyn Jones Ana Fernandez Gonzalez Olga Kofanova Johanna Trouet Pamela Pinzani Stefania Gelmini Serena Bonin Carole Foy Source Type: research
Interlaboratory evaluation of quality control methods for circulating cell-free DNA extraction
In this study we evaluated the application of nucleic acid "spike-in" control materials to aid quality control (QC) and standardisation of cfDNA isolation for use in in vitro diagnostic assays. We describe an approach for the design and characterisation of in-process QC materials, illustrating it with a spike-in material containing an exogenous Arabidopsis sequence and DNA fragments approximating to ccfDNA and genomic DNA lengths. Protocols for inclusion of the spike-in material in plasma ccfDNA extraction and quantification of its recovery by digital PCR (dPCR) were assessed for their suitability for process QC in an inte...
Source: New Biotechnology - September 20, 2023 Category: Biotechnology Authors: Alison Devonshire Gerwyn Jones Ana Fernandez Gonzalez Olga Kofanova Johanna Trouet Pamela Pinzani Stefania Gelmini Serena Bonin Carole Foy Source Type: research
Interlaboratory evaluation of quality control methods for circulating cell-free DNA extraction
In this study we evaluated the application of nucleic acid "spike-in" control materials to aid quality control (QC) and standardisation of cfDNA isolation for use in in vitro diagnostic assays. We describe an approach for the design and characterisation of in-process QC materials, illustrating it with a spike-in material containing an exogenous Arabidopsis sequence and DNA fragments approximating to ccfDNA and genomic DNA lengths. Protocols for inclusion of the spike-in material in plasma ccfDNA extraction and quantification of its recovery by digital PCR (dPCR) were assessed for their suitability for process QC in an inte...
Source: New Biotechnology - September 20, 2023 Category: Biotechnology Authors: Alison Devonshire Gerwyn Jones Ana Fernandez Gonzalez Olga Kofanova Johanna Trouet Pamela Pinzani Stefania Gelmini Serena Bonin Carole Foy Source Type: research
Transcription factor-based biosensors for detection of naturally occurring phenolic acids
N Biotechnol. 2023 Sep 13:S1871-6784(23)00047-X. doi: 10.1016/j.nbt.2023.09.004. Online ahead of print.ABSTRACTPhenolic acids including hydroxybenzoic and hydroxycinnamic acids are secondary plant and fungal metabolites involved in many physiological processes offering health and dietary benefits. They are often utilised as precursors for production of value-added compounds. The limited availability of synthetic biology tools, such as whole-cell biosensors suitable for monitoring the dynamics of phenolic acids intracellularly and extracellularly, hinders the capabilities to develop high-throughput screens to study their me...
Source: New Biotechnology - September 15, 2023 Category: Biotechnology Authors: Ernesta Augustiniene Ingrida Kutraite Egle Valanciene Paulius Matulis Ilona Jonuskiene Naglis Malys Source Type: research
Transcription factor-based biosensors for detection of naturally occurring phenolic acids
N Biotechnol. 2023 Sep 13:S1871-6784(23)00047-X. doi: 10.1016/j.nbt.2023.09.004. Online ahead of print.ABSTRACTPhenolic acids including hydroxybenzoic and hydroxycinnamic acids are secondary plant and fungal metabolites involved in many physiological processes offering health and dietary benefits. They are often utilised as precursors for production of value-added compounds. The limited availability of synthetic biology tools, such as whole-cell biosensors suitable for monitoring the dynamics of phenolic acids intracellularly and extracellularly, hinders the capabilities to develop high-throughput screens to study their me...
Source: New Biotechnology - September 15, 2023 Category: Biotechnology Authors: Ernesta Augustiniene Ingrida Kutraite Egle Valanciene Paulius Matulis Ilona Jonuskiene Naglis Malys Source Type: research
Transcription factor-based biosensors for detection of naturally occurring phenolic acids
N Biotechnol. 2023 Sep 13:S1871-6784(23)00047-X. doi: 10.1016/j.nbt.2023.09.004. Online ahead of print.ABSTRACTPhenolic acids including hydroxybenzoic and hydroxycinnamic acids are secondary plant and fungal metabolites involved in many physiological processes offering health and dietary benefits. They are often utilised as precursors for production of value-added compounds. The limited availability of synthetic biology tools, such as whole-cell biosensors suitable for monitoring the dynamics of phenolic acids intracellularly and extracellularly, hinders the capabilities to develop high-throughput screens to study their me...
Source: New Biotechnology - September 15, 2023 Category: Biotechnology Authors: Ernesta Augustiniene Ingrida Kutraite Egle Valanciene Paulius Matulis Ilona Jonuskiene Naglis Malys Source Type: research
Transcription factor-based biosensors for detection of naturally occurring phenolic acids
N Biotechnol. 2023 Sep 13:S1871-6784(23)00047-X. doi: 10.1016/j.nbt.2023.09.004. Online ahead of print.ABSTRACTPhenolic acids including hydroxybenzoic and hydroxycinnamic acids are secondary plant and fungal metabolites involved in many physiological processes offering health and dietary benefits. They are often utilised as precursors for production of value-added compounds. The limited availability of synthetic biology tools, such as whole-cell biosensors suitable for monitoring the dynamics of phenolic acids intracellularly and extracellularly, hinders the capabilities to develop high-throughput screens to study their me...
Source: New Biotechnology - September 15, 2023 Category: Biotechnology Authors: Ernesta Augustiniene Ingrida Kutraite Egle Valanciene Paulius Matulis Ilona Jonuskiene Naglis Malys Source Type: research
Approaches for high-throughput quantification of periplasmic recombinant proteins
N Biotechnol. 2023 Sep 12;77:149-160. doi: 10.1016/j.nbt.2023.09.003. Online ahead of print.ABSTRACTThe Gram-negative periplasm is a convenient location for the accumulation of many recombinant proteins including biopharmaceutical products. It is the site of disulphide bond formation, required by some proteins (such as antibody fragments) for correct folding and function. It also permits simpler protein release and downstream processing than cytoplasmic accumulation. As such, targeting of recombinant proteins to the E. coli periplasm is a key strategy in biologic manufacture. However, expression and translocation of each r...
Source: New Biotechnology - September 14, 2023 Category: Biotechnology Authors: Alexander Osgerby Tim W Overton Source Type: research
Approaches for High-throughput Quantification of Periplasmic Recombinant Proteins
N Biotechnol. 2023 Sep 12:S1871-6784(23)00046-8. doi: 10.1016/j.nbt.2023.09.003. Online ahead of print.ABSTRACTThe Gram-negative periplasm is a convenient location for the accumulation of many recombinant proteins including biopharmaceutical products. It is the site of disulphide bond formation, required by some proteins (such as antibody fragments) for correct folding and function. It also permits simpler protein release and downstream processing than cytoplasmic accumulation. As such, targeting of recombinant proteins to the E. coli periplasm is a key strategy in biologic manufacture. However, expression and translocatio...
Source: New Biotechnology - September 14, 2023 Category: Biotechnology Authors: Alexander Osgerby Tim W Overton Source Type: research
Biomaterials Text Mining: A Hands-On Comparative Study of Methods on Polydioxanone Biocompatibility
N Biotechnol. 2023 Sep 4:S1871-6784(23)00044-4. doi: 10.1016/j.nbt.2023.09.001. Online ahead of print.ABSTRACTScientific information extraction is fundamental for research and innovation, but is currently mostly a manual, time-consuming process. Text Mining tools (TMTs) enable automated, accurate and quick information extraction from text, but there is little precedent of their use in the biomaterials field. Here, we compare the ability of various TMTs to extract useful information from biomaterials abstracts. Focusing on the biocompatibility of polydioxanone, a biodegradable polymer for which there are relatively few scie...
Source: New Biotechnology - September 6, 2023 Category: Biotechnology Authors: Carla V Fuentesl ópez Austin McKitrick Javier Corvi Maria-Pau Ginebra Osnat Hakimi Source Type: research
Affibody-based hBCMA x CD16 dual engagers for NK cell-mediated killing of multiple myeloma cells
We describe the development and characterization of the (to date) smallest Natural Killer (NK) cell re-directing human B Cell Maturation Antigen (hBCMA) x CD16 dual engagers for potential treatment of multiple myeloma, based on combinations of small 58 amino acid, non-immunoglobulin, affibody affinity proteins. Affibody molecules to human CD16a were selected from a combinatorial library by phage display resulting in the identification of three unique binders with affinities (KD) for CD16a in the range of 100 nM-3 µM. The affibody exhibiting the highest affinity demonstrated insensitivity towards the CD16a allotype (158F/V...
Source: New Biotechnology - September 6, 2023 Category: Biotechnology Authors: Kim Anh Giang Thorstein Boxaspen Yumei Diao Johan Nilvebrant Mizuha Kosugi-Kanaya Minoru Kanaya Silje Zandstra Krokeide Fredrik Lehmann Stefan Svensson Gelius Karl-Johan Malmberg Per- Åke Nygren Source Type: research
Biomaterials Text Mining: A Hands-On Comparative Study of Methods on Polydioxanone Biocompatibility
N Biotechnol. 2023 Sep 4:S1871-6784(23)00044-4. doi: 10.1016/j.nbt.2023.09.001. Online ahead of print.ABSTRACTScientific information extraction is fundamental for research and innovation, but is currently mostly a manual, time-consuming process. Text Mining tools (TMTs) enable automated, accurate and quick information extraction from text, but there is little precedent of their use in the biomaterials field. Here, we compare the ability of various TMTs to extract useful information from biomaterials abstracts. Focusing on the biocompatibility of polydioxanone, a biodegradable polymer for which there are relatively few scie...
Source: New Biotechnology - September 6, 2023 Category: Biotechnology Authors: Carla V Fuentesl ópez Austin McKitrick Javier Corvi Maria-Pau Ginebra Osnat Hakimi Source Type: research
Affibody-based hBCMA x CD16 dual engagers for NK cell-mediated killing of multiple myeloma cells
We describe the development and characterization of the (to date) smallest Natural Killer (NK) cell re-directing human B Cell Maturation Antigen (hBCMA) x CD16 dual engagers for potential treatment of multiple myeloma, based on combinations of small 58 amino acid, non-immunoglobulin, affibody affinity proteins. Affibody molecules to human CD16a were selected from a combinatorial library by phage display resulting in the identification of three unique binders with affinities (KD) for CD16a in the range of 100 nM-3 µM. The affibody exhibiting the highest affinity demonstrated insensitivity towards the CD16a allotype (158F/V...
Source: New Biotechnology - September 6, 2023 Category: Biotechnology Authors: Kim Anh Giang Thorstein Boxaspen Yumei Diao Johan Nilvebrant Mizuha Kosugi-Kanaya Minoru Kanaya Silje Zandstra Krokeide Fredrik Lehmann Stefan Svensson Gelius Karl-Johan Malmberg Per- Åke Nygren Source Type: research
Biomaterials Text Mining: A Hands-On Comparative Study of Methods on Polydioxanone Biocompatibility
N Biotechnol. 2023 Sep 4:S1871-6784(23)00044-4. doi: 10.1016/j.nbt.2023.09.001. Online ahead of print.ABSTRACTScientific information extraction is fundamental for research and innovation, but is currently mostly a manual, time-consuming process. Text Mining tools (TMTs) enable automated, accurate and quick information extraction from text, but there is little precedent of their use in the biomaterials field. Here, we compare the ability of various TMTs to extract useful information from biomaterials abstracts. Focusing on the biocompatibility of polydioxanone, a biodegradable polymer for which there are relatively few scie...
Source: New Biotechnology - September 6, 2023 Category: Biotechnology Authors: Carla V Fuentesl ópez Austin McKitrick Javier Corvi Maria-Pau Ginebra Osnat Hakimi Source Type: research
