Novel GH109 enzymes for bioconversion of group A red blood cells to the universal donor group O
N Biotechnol. 2023 Aug 27:S1871-6784(23)00041-9. doi: 10.1016/j.nbt.2023.08.002. Online ahead of print.ABSTRACTGlycoside hydrolases (GHs) have been employed for industrial and biotechnological purposes and often play an important role in new applications. The red blood cell (RBC) antigen system depends on the composition of oligosaccharides on the surface of erythrocytes, thus defining the ABO blood type classification. Incorrect blood transfusions may lead to fatal consequences, making the availability of the correct blood group critical. In this regard, it has been demonstrated that some GHs may be helpful in the convers...
Source: New Biotechnology - August 29, 2023 Category: Biotechnology Authors: Nicola Curci Roberta Iacono Dorotea Raventos Segura Michele Cillo Beatrice Cobucci-Ponzano Andrea Strazzulli Antonio Leonardi Lars Giger Marco Moracci Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14;77:100-110. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and effective r...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14;77:100-110. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and effective r...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Replication-competent influenza virus with a protein-responsive multiplication ability
N Biotechnol. 2023 Aug 14:S1871-6784(23)00040-7. doi: 10.1016/j.nbt.2023.08.001. Online ahead of print.ABSTRACTApplications of influenza A viruses (IAV) for virotherapy and biotechnology have accelerated substantially with the development of reverse genetic technology and advances in the understanding of packaging signals. While the use of a replication-competent IAV is particularly promising, owing to its efficient transmission to organ depths with high infectivity, there is also a risk that its multiplication cannot be controlled in a cell-type-specific manner, causing an infectious disease. Therefore, here a simple and ...
Source: New Biotechnology - August 16, 2023 Category: Biotechnology Authors: Shinzi Ogasawara Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
