Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: a case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: a case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: a case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17:S1871-6784(23)00037-7. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 d...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: a case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: the periodic hydrodynamic loading and the shear stress accumulation
N Biotechnol. 2023 Jul 11:S1871-6784(23)00036-5. doi: 10.1016/j.nbt.2023.07.002. Online ahead of print.ABSTRACTIn vitro production of blood platelets for transfusion purposes is gaining interest. While platelet production is now possible on a laboratory scale, the challenge is to move towards industrial production. Attaining this goal calls for the development of platelet release devices capable of producing large quantities of platelets. To this end, we have developed a continuous-flow platelet release device composed of five spherical chambers each containing two calibrated cones placed in a staggered configuration. Foll...
Source: New Biotechnology - July 13, 2023 Category: Biotechnology Authors: Ana ïs Pongérard L éa Mallo Valentin Do Sacramento Olivier Boiron Anita Eckly Christian Gachet Fran çois Lanza Yannick Knapp Catherine Strassel Source Type: research
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: the periodic hydrodynamic loading and the shear stress accumulation
N Biotechnol. 2023 Jul 11:S1871-6784(23)00036-5. doi: 10.1016/j.nbt.2023.07.002. Online ahead of print.ABSTRACTIn vitro production of blood platelets for transfusion purposes is gaining interest. While platelet production is now possible on a laboratory scale, the challenge is to move towards industrial production. Attaining this goal calls for the development of platelet release devices capable of producing large quantities of platelets. To this end, we have developed a continuous-flow platelet release device composed of five spherical chambers each containing two calibrated cones placed in a staggered configuration. Foll...
Source: New Biotechnology - July 13, 2023 Category: Biotechnology Authors: Ana ïs Pongérard L éa Mallo Valentin Do Sacramento Olivier Boiron Anita Eckly Christian Gachet Fran çois Lanza Yannick Knapp Catherine Strassel Source Type: research
