Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
Influence of culture media composition on the rheology of microalgae concentrates on a large scale
This study aimed to characterize microalgal concentrates rheologically in different culture media. The presence of bacteria was quantified by photorespirometry and plate counting techniques. The culture medium was found to significantly influence viscosity, with primary wastewater exhibiting the highest viscosity and seawater plus pig slurry the lowest. The concentration of heterotrophic bacteria was directly related to the viscosity. Extracellular polysaccharides (EPS) in supernatant exhibited an inverse viscosity trend compared to biomass concentrates, with pig slurry cultures having higher concentrations. These findings...
Source: New Biotechnology - August 2, 2023 Category: Biotechnology Authors: Solaima Belachqer-El Attar Ainoa Morillas-Espa ña Ana S ánchez-Zurano Luiggi Cavalcanti Pess ôa Mar ía Guadalupe Pinna-Hernández Denilson de Jesus Assis Jos é Luis Casas López Gabriel Aci én Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
In this study, we propose PAS, a polypeptide sequence composed of repeated Pro, Ala, and Ser residues, as a universal masking sequence. PAS has no specificity, but can inhibit antibody binding through steric hindrance caused by its large fluid dynamic radius and disordered structure; additionally, its length can be adjusted. We fused PAS to the N-terminus of an anti-CD3 single-chain variable fragment (scFv) and a bsAb, that targets both the epidermal growth factor receptor and CD3, via a recognition sequence cleaved by cancer-related proteases. PAS integration inhibited anti-CD3 scFv binding with higher efficacy than the e...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: Atsushi Maejima Saori Suzuki Koki Makabe Izumi Kumagai Ryutaro Asano Source Type: research
An artificial intelligence approach for identification of microalgae cultures
N Biotechnol. 2023 Jul 17;77:58-67. doi: 10.1016/j.nbt.2023.07.003. Online ahead of print.ABSTRACTIn this work, a model for the characterization of microalgae cultures based on artificial neural networks has been developed. The characterization of microalgae cultures is essential to guarantee the quality of the biomass, and the objective of this work is to achieve a simple and fast method to address this issue. Data acquisition was performed using FlowCam, a device capable of capturing images of the cells detected in a culture sample, which are used as inputs by the model. The model can distinguish between 6 different gene...
Source: New Biotechnology - July 19, 2023 Category: Biotechnology Authors: P Ot álora J L Guzm án F G Aci én M Berenguel A Reul Source Type: research
