Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control
N Biotechnol. 2023 Nov 30;79:1-19. doi: 10.1016/j.nbt.2023.11.003. Online ahead of print.ABSTRACTMammalian cells have developed dedicated molecular mechanisms to tightly control expression levels of their genes where the specific transcriptomic signature across all genes eventually determines the cell's phenotype. Modulating cellular phenotypes is of major interest to study their role in disease or to reprogram cells for the manufacturing of recombinant products, such as biopharmaceuticals. Cells of mammalian origin, for example Chinese hamster ovary (CHO) and Human embryonic kidney 293 (HEK293) cells, are most commonly em...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Peter Eisenhut Nicolas Marx Giulia Borsi Maja Pape ž Caterina Ruggeri Martina Baumann Nicole Borth Source Type: research
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential
N Biotechnol. 2023 Nov 29:S1871-6784(23)00067-5. doi: 10.1016/j.nbt.2023.11.004. Online ahead of print.ABSTRACTMicrobial communities have long been observed in oil reservoirs, where the subsurface conditions are major drivers shaping their structure and functions. Furthermore, anthropogenic activities such as water flooding during oil production can affect microbial activities and community compositions in oil reservoirs through the injection of recycled produced water, often associated with biocides. However, it is still unclear to what extent the introduced chemicals and microbes influence the metabolic potential of the ...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Fatou T Mbow Ali Akbari Nicole Dopffel Konstantin Schneider Soujatya Mukherjee Rainer U Meckenstock Source Type: research
Artificial intelligence / machine-learning tool for post-market surveillance of in vitro diagnostic assays
N Biotechnol. 2023 Nov 29:S1871-6784(23)00068-7. doi: 10.1016/j.nbt.2023.11.005. Online ahead of print.ABSTRACTThe study compares an artificial intelligence technology with traditional manual search of literature databases to assess the accuracy and efficiency of retrieving relevant articles for post-market surveillance of in vitro diagnostic and medical devices under the Medical Device Regulation and In Vitro Diagnostic Medical Device Regulation. Over a 3-year period, literature searches and technical assessment searches were performed manually or using the Huma.AI platform to retrieve relevant articles related to the saf...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Joanna Reniewicz Vinay Suryaprakash Justyna Kowalczyk Anna Blacha Greg Kostello Haiming Tan Yan Wang Patrick Reineke Davide Manissero Source Type: research
Manipulating gene expression levels in mammalian cell factories: An outline of synthetic molecular toolboxes to achieve multiplexed control
N Biotechnol. 2023 Nov 30;79:1-19. doi: 10.1016/j.nbt.2023.11.003. Online ahead of print.ABSTRACTMammalian cells have developed dedicated molecular mechanisms to tightly control expression levels of their genes where the specific transcriptomic signature across all genes eventually determines the cell's phenotype. Modulating cellular phenotypes is of major interest to study their role in disease or to reprogram cells for the manufacturing of recombinant products, such as biopharmaceuticals. Cells of mammalian origin, for example Chinese hamster ovary (CHO) and Human embryonic kidney 293 (HEK293) cells, are most commonly em...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Peter Eisenhut Nicolas Marx Giulia Borsi Maja Pape ž Caterina Ruggeri Martina Baumann Nicole Borth Source Type: research
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential
N Biotechnol. 2023 Nov 29:S1871-6784(23)00067-5. doi: 10.1016/j.nbt.2023.11.004. Online ahead of print.ABSTRACTMicrobial communities have long been observed in oil reservoirs, where the subsurface conditions are major drivers shaping their structure and functions. Furthermore, anthropogenic activities such as water flooding during oil production can affect microbial activities and community compositions in oil reservoirs through the injection of recycled produced water, often associated with biocides. However, it is still unclear to what extent the introduced chemicals and microbes influence the metabolic potential of the ...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Fatou T Mbow Ali Akbari Nicole Dopffel Konstantin Schneider Soujatya Mukherjee Rainer U Meckenstock Source Type: research
Artificial intelligence / machine-learning tool for post-market surveillance of in vitro diagnostic assays
N Biotechnol. 2023 Nov 29:S1871-6784(23)00068-7. doi: 10.1016/j.nbt.2023.11.005. Online ahead of print.ABSTRACTThe study compares an artificial intelligence technology with traditional manual search of literature databases to assess the accuracy and efficiency of retrieving relevant articles for post-market surveillance of in vitro diagnostic and medical devices under the Medical Device Regulation and In Vitro Diagnostic Medical Device Regulation. Over a 3-year period, literature searches and technical assessment searches were performed manually or using the Huma.AI platform to retrieve relevant articles related to the saf...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Joanna Reniewicz Vinay Suryaprakash Justyna Kowalczyk Anna Blacha Greg Kostello Haiming Tan Yan Wang Patrick Reineke Davide Manissero Source Type: research
Manipulating gene expression levels in mammalian cell factories: an outline of synthetic molecular toolboxes to achieve multiplexed control
N Biotechnol. 2023 Nov 29:S1871-6784(23)00066-3. doi: 10.1016/j.nbt.2023.11.003. Online ahead of print.ABSTRACTCells, both of prokaryotic and eukaryotic origin, have developed dedicated molecular mechanisms to tightly control expression levels of their genes where the specific transcriptomic signature across all genes eventually determines the cell phenotype. Modulating cellular phenotypes is of major interest, either to study their role in disease or to reprogram cells for the manufacture of recombinant products, such as biopharmaceuticals. For the latter, cells of mammalian origin, such as Chinese hamster ovary (CHO) and...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Peter Eisenhut Nicolas Marx Giulia Borsi Maja Pape ž Caterina Ruggeri Martina Baumann Nicole Borth Source Type: research
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential
N Biotechnol. 2023 Nov 29:S1871-6784(23)00067-5. doi: 10.1016/j.nbt.2023.11.004. Online ahead of print.ABSTRACTMicrobial communities have long been observed in oil reservoirs, where the subsurface conditions are major drivers shaping their structure and functions. Furthermore, anthropogenic activities such as water flooding during oil production can affect microbial activities and community compositions in oil reservoirs through the injection of recycled produced water, often associated with biocides. However, it is still unclear to what extent the introduced chemicals and microbes influence the metabolic potential of the ...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Fatou T Mbow Ali Akbari Nicole Dopffel Konstantin Schneider Soujatya Mukherjee Rainer U Meckenstock Source Type: research
Artificial intelligence / machine-learning tool for post-market surveillance of in vitro diagnostic assays
N Biotechnol. 2023 Nov 29:S1871-6784(23)00068-7. doi: 10.1016/j.nbt.2023.11.005. Online ahead of print.ABSTRACTThe study compares an artificial intelligence technology with traditional manual search of literature databases to assess the accuracy and efficiency of retrieving relevant articles for post-market surveillance of in vitro diagnostic and medical devices under the Medical Device Regulation and In Vitro Diagnostic Medical Device Regulation. Over a 3-year period, literature searches and technical assessment searches were performed manually or using the Huma.AI platform to retrieve relevant articles related to the saf...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Joanna Reniewicz Vinay Suryaprakash Justyna Kowalczyk Anna Blacha Greg Kostello Haiming Tan Yan Wang Patrick Reineke Davide Manissero Source Type: research
Manipulating gene expression levels in mammalian cell factories: an outline of synthetic molecular toolboxes to achieve multiplexed control
N Biotechnol. 2023 Nov 29:S1871-6784(23)00066-3. doi: 10.1016/j.nbt.2023.11.003. Online ahead of print.ABSTRACTCells, both of prokaryotic and eukaryotic origin, have developed dedicated molecular mechanisms to tightly control expression levels of their genes where the specific transcriptomic signature across all genes eventually determines the cell phenotype. Modulating cellular phenotypes is of major interest, either to study their role in disease or to reprogram cells for the manufacture of recombinant products, such as biopharmaceuticals. For the latter, cells of mammalian origin, such as Chinese hamster ovary (CHO) and...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Peter Eisenhut Nicolas Marx Giulia Borsi Maja Pape ž Caterina Ruggeri Martina Baumann Nicole Borth Source Type: research
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential
N Biotechnol. 2023 Nov 29:S1871-6784(23)00067-5. doi: 10.1016/j.nbt.2023.11.004. Online ahead of print.ABSTRACTMicrobial communities have long been observed in oil reservoirs, where the subsurface conditions are major drivers shaping their structure and functions. Furthermore, anthropogenic activities such as water flooding during oil production can affect microbial activities and community compositions in oil reservoirs through the injection of recycled produced water, often associated with biocides. However, it is still unclear to what extent the introduced chemicals and microbes influence the metabolic potential of the ...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Fatou T Mbow Ali Akbari Nicole Dopffel Konstantin Schneider Soujatya Mukherjee Rainer U Meckenstock Source Type: research
Artificial intelligence / machine-learning tool for post-market surveillance of in vitro diagnostic assays
N Biotechnol. 2023 Nov 29:S1871-6784(23)00068-7. doi: 10.1016/j.nbt.2023.11.005. Online ahead of print.ABSTRACTThe study compares an artificial intelligence technology with traditional manual search of literature databases to assess the accuracy and efficiency of retrieving relevant articles for post-market surveillance of in vitro diagnostic and medical devices under the Medical Device Regulation and In Vitro Diagnostic Medical Device Regulation. Over a 3-year period, literature searches and technical assessment searches were performed manually or using the Huma.AI platform to retrieve relevant articles related to the saf...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Joanna Reniewicz Vinay Suryaprakash Justyna Kowalczyk Anna Blacha Greg Kostello Haiming Tan Yan Wang Patrick Reineke Davide Manissero Source Type: research
Manipulating gene expression levels in mammalian cell factories: an outline of synthetic molecular toolboxes to achieve multiplexed control
N Biotechnol. 2023 Nov 29:S1871-6784(23)00066-3. doi: 10.1016/j.nbt.2023.11.003. Online ahead of print.ABSTRACTCells, both of prokaryotic and eukaryotic origin, have developed dedicated molecular mechanisms to tightly control expression levels of their genes where the specific transcriptomic signature across all genes eventually determines the cell phenotype. Modulating cellular phenotypes is of major interest, either to study their role in disease or to reprogram cells for the manufacture of recombinant products, such as biopharmaceuticals. For the latter, cells of mammalian origin, such as Chinese hamster ovary (CHO) and...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Peter Eisenhut Nicolas Marx Giulia Borsi Maja Pape ž Caterina Ruggeri Martina Baumann Nicole Borth Source Type: research
Insights into the effects of anthropogenic activities on oil reservoir microbiome and metabolic potential
N Biotechnol. 2023 Nov 29:S1871-6784(23)00067-5. doi: 10.1016/j.nbt.2023.11.004. Online ahead of print.ABSTRACTMicrobial communities have long been observed in oil reservoirs, where the subsurface conditions are major drivers shaping their structure and functions. Furthermore, anthropogenic activities such as water flooding during oil production can affect microbial activities and community compositions in oil reservoirs through the injection of recycled produced water, often associated with biocides. However, it is still unclear to what extent the introduced chemicals and microbes influence the metabolic potential of the ...
Source: New Biotechnology - December 1, 2023 Category: Biotechnology Authors: Fatou T Mbow Ali Akbari Nicole Dopffel Konstantin Schneider Soujatya Mukherjee Rainer U Meckenstock Source Type: research
Effect of seawater on the biomass composition of Spirulina produced at a pilot-scale
N Biotechnol. 2023 Nov 14;78:173-179. doi: 10.1016/j.nbt.2023.11.002. Online ahead of print.ABSTRACTThe microalga Arthrospira platensis BEA 005B was produced in 11.4 m3 raceway photobioreactors and a culture medium based on commercial fertilisers and either freshwater or seawater. The biomass productivity of the reactors operated at a fixed dilution rate of 0.3 day-1 decreased from 22.9 g·m-2·day-1 when operated using freshwater to 16.3 g·m-2·day-1 when the biomass was produced using seawater. The protein content of the biomass produced in seawater was lower; however, the content of essential amino acids including vali...
Source: New Biotechnology - November 15, 2023 Category: Biotechnology Authors: Silvia Villar ó Marco Garc ía-Vaquero Lara Mor án Carlos Álvarez Eduarda Melo Cabral Tomas Lafarga Source Type: research
